AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780036289.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036289.01.T01 MTR_7g010930 99.078 217 2 0 1 217 1 217 5.20e-159 438
MsG0780036289.01.T01 MTR_6g012390 64.186 215 62 2 18 217 1 215 7.23e-95 276
MsG0780036289.01.T01 MTR_2g021720 42.574 101 46 2 120 217 103 194 6.62e-16 73.2
MsG0780036289.01.T01 MTR_2g021720 42.574 101 46 2 120 217 103 194 1.19e-15 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036289.01.T01 AT3G01170 51.339 224 93 5 1 217 1 215 7.15e-67 205
MsG0780036289.01.T01 AT5G15260 51.261 238 91 8 1 217 1 234 9.29e-67 205
MsG0780036289.01.T01 AT5G15260 51.261 238 91 8 1 217 1 234 9.29e-67 205
MsG0780036289.01.T01 AT5G19025 54.082 98 43 1 120 217 150 245 9.53e-26 100
MsG0780036289.01.T01 AT5G19025 53.608 97 43 1 120 216 150 244 4.25e-25 99.4
MsG0780036289.01.T01 AT3G06180 53.061 98 44 1 120 217 132 227 7.28e-25 98.2
MsG0780036289.01.T01 AT3G01170 39.216 102 56 2 1 97 1 101 2.16e-11 60.1

Find 50 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGCTGTTGTTATTGCTTT+AGG 0.138895 7:-6029750 MsG0780036289.01.T01:CDS
CAAACTTTGTGGTTTGTTTA+TGG 0.153027 7:-6029682 MsG0780036289.01.T01:CDS
TTGATCTTTCTATTCTTCTT+TGG 0.201359 7:+6029288 None:intergenic
TGTTATTGCTTTAGGGTTCT+TGG 0.201503 7:-6029742 MsG0780036289.01.T01:CDS
TAGCTGTTGTTATTGCTTTA+GGG 0.217546 7:-6029749 MsG0780036289.01.T01:CDS
TTCTTCAATCCCTTACAATT+AGG 0.224249 7:+6029537 None:intergenic
GAATAGAAAGATCAAGAAAT+AGG 0.263550 7:-6029280 None:intergenic
CTCATCACAAATTGAATATA+TGG 0.264029 7:+6029714 None:intergenic
TCTTCAATCCCTTACAATTA+GGG 0.277107 7:+6029538 None:intergenic
TGTTGGTAGGTTAGAAGTTC+CGG 0.283392 7:-6029313 MsG0780036289.01.T01:CDS
AAGAAGATGGCTCCTGTTAA+TGG 0.295596 7:-6029375 MsG0780036289.01.T01:CDS
AGATGGTGGTGGAATTAAAA+AGG 0.303114 7:-6029448 MsG0780036289.01.T01:CDS
TTGTGGTTTGTTTATGGATT+TGG 0.314324 7:-6029676 MsG0780036289.01.T01:CDS
CATGTGCTGTTGTTGCTACA+TGG 0.391115 7:-6029596 MsG0780036289.01.T01:CDS
GTTGGTAGGTTAGAAGTTCC+GGG 0.396075 7:-6029312 MsG0780036289.01.T01:CDS
AGTGAAAGTTTCAAACTTTG+TGG 0.397466 7:-6029693 MsG0780036289.01.T01:CDS
CCTGTGATTTATGTATCTCT+TGG 0.405703 7:-6029630 MsG0780036289.01.T01:CDS
AGAACAGCTCTTCCATTAAC+AGG 0.409782 7:+6029363 None:intergenic
ATGTGGGAACCCTAATTGTA+AGG 0.424650 7:-6029547 MsG0780036289.01.T01:CDS
CGAATTCTTCACACAATCTT+CGG 0.436293 7:+6029485 None:intergenic
TGTGAAGAATTCGCCGTCGT+TGG 0.440660 7:-6029475 MsG0780036289.01.T01:CDS
AGAGCTGTTCTAGTTCTTAG+AGG 0.448409 7:-6029351 MsG0780036289.01.T01:CDS
CAACAGATGAGATCAAACTA+TGG 0.452999 7:+6029901 None:intergenic
GTGAAGAATTCGCCGTCGTT+GGG 0.455176 7:-6029474 MsG0780036289.01.T01:CDS
AGTGTGGTTGTTCTGTTGGT+AGG 0.472839 7:-6029326 MsG0780036289.01.T01:CDS
GGAAAGTGTGGTTGTTCTGT+TGG 0.477218 7:-6029330 MsG0780036289.01.T01:CDS
GAAGAGAATTGAGTGTCTTA+CGG 0.499754 7:+6029826 None:intergenic
TCGATCCGCGGTTGTTGATG+TGG 0.500037 7:-6029781 MsG0780036289.01.T01:CDS
CTTATACATCAAGGAAATGT+GGG 0.514031 7:-6029563 MsG0780036289.01.T01:CDS
TTTCTATTCTTCTTTGGACC+CGG 0.518411 7:+6029294 None:intergenic
CGAAGCCGAGCTTAAGAAGA+TGG 0.524432 7:-6029388 MsG0780036289.01.T01:CDS
AGGAGCCATCTTCTTAAGCT+CGG 0.536872 7:+6029383 None:intergenic
TCGCCGTCGTTGGGTAAAGA+TGG 0.539719 7:-6029465 MsG0780036289.01.T01:CDS
GCTTATACATCAAGGAAATG+TGG 0.544775 7:-6029564 MsG0780036289.01.T01:CDS
TGTGGGAACCCTAATTGTAA+GGG 0.554639 7:-6029546 MsG0780036289.01.T01:CDS
TCGCCATGGATGATGATGAT+TGG 0.560084 7:+6029877 None:intergenic
CTAGTTCTTAGAGGAAAGTG+TGG 0.579987 7:-6029342 MsG0780036289.01.T01:CDS
TCCTCGATTTAGCTAAATCG+AGG 0.583685 7:+6029850 None:intergenic
CCAAGAGATACATAAATCAC+AGG 0.588766 7:+6029630 None:intergenic
CCCTCGATTTAGCTAAATCG+AGG 0.592975 7:-6029851 MsG0780036289.01.T01:CDS
TGACCAATCATCATCATCCA+TGG 0.601187 7:-6029880 MsG0780036289.01.T01:CDS
CCGTCGTTGGGTAAAGATGG+TGG 0.619641 7:-6029462 MsG0780036289.01.T01:CDS
CCTCGATTTAGCTAAATCGA+GGG 0.621163 7:+6029851 None:intergenic
GATCGAGATCGCTCGCACTG+CGG 0.651752 7:+6029798 None:intergenic
TCGTTGGGTAAAGATGGTGG+TGG 0.659209 7:-6029459 MsG0780036289.01.T01:CDS
AGTTCGCGATGATGATCACA+AGG 0.663527 7:+6029411 None:intergenic
GTGCGAGCGATCTCGATCCG+CGG 0.666573 7:-6029793 MsG0780036289.01.T01:CDS
TAAATCGAGGGAATTCGCCA+TGG 0.679775 7:+6029863 None:intergenic
CCACCATCTTTACCCAACGA+CGG 0.694775 7:+6029462 None:intergenic
TATAACCACATCAACAACCG+CGG 0.698235 7:+6029776 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATAGCTTATACATCA+AGG - Chr7:6029621-6029640 MsG0780036289.01.T01:CDS 20.0%
! CTCATCACAAATTGAATATA+TGG + Chr7:6029482-6029501 None:intergenic 25.0%
! TTGATCTTTCTATTCTTCTT+TGG + Chr7:6029908-6029927 None:intergenic 25.0%
AGTGAAAGTTTCAAACTTTG+TGG - Chr7:6029500-6029519 MsG0780036289.01.T01:CDS 30.0%
CTTATACATCAAGGAAATGT+GGG - Chr7:6029630-6029649 MsG0780036289.01.T01:CDS 30.0%
TCTTCAATCCCTTACAATTA+GGG + Chr7:6029658-6029677 None:intergenic 30.0%
TTCTTCAATCCCTTACAATT+AGG + Chr7:6029659-6029678 None:intergenic 30.0%
! ATAGCTGTTGTTATTGCTTT+AGG - Chr7:6029443-6029462 MsG0780036289.01.T01:CDS 30.0%
! TAGCTGTTGTTATTGCTTTA+GGG - Chr7:6029444-6029463 MsG0780036289.01.T01:CDS 30.0%
!! CAAACTTTGTGGTTTGTTTA+TGG - Chr7:6029511-6029530 MsG0780036289.01.T01:CDS 30.0%
!! TTGTGGTTTGTTTATGGATT+TGG - Chr7:6029517-6029536 MsG0780036289.01.T01:CDS 30.0%
CAACAGATGAGATCAAACTA+TGG + Chr7:6029295-6029314 None:intergenic 35.0%
CCAAGAGATACATAAATCAC+AGG + Chr7:6029566-6029585 None:intergenic 35.0%
CCTGTGATTTATGTATCTCT+TGG - Chr7:6029563-6029582 MsG0780036289.01.T01:CDS 35.0%
CGAATTCTTCACACAATCTT+CGG + Chr7:6029711-6029730 None:intergenic 35.0%
GAAGAGAATTGAGTGTCTTA+CGG + Chr7:6029370-6029389 None:intergenic 35.0%
GCTTATACATCAAGGAAATG+TGG - Chr7:6029629-6029648 MsG0780036289.01.T01:CDS 35.0%
TTTCTATTCTTCTTTGGACC+CGG + Chr7:6029902-6029921 None:intergenic 35.0%
!! AGATGGTGGTGGAATTAAAA+AGG - Chr7:6029745-6029764 MsG0780036289.01.T01:CDS 35.0%
!! GATGGTGGTGGAATTAAAAA+GGG - Chr7:6029746-6029765 MsG0780036289.01.T01:CDS 35.0%
!! TGTTATTGCTTTAGGGTTCT+TGG - Chr7:6029451-6029470 MsG0780036289.01.T01:CDS 35.0%
AAGAAGATGGCTCCTGTTAA+TGG - Chr7:6029818-6029837 MsG0780036289.01.T01:CDS 40.0%
AGAACAGCTCTTCCATTAAC+AGG + Chr7:6029833-6029852 None:intergenic 40.0%
ATGTGGGAACCCTAATTGTA+AGG - Chr7:6029646-6029665 MsG0780036289.01.T01:CDS 40.0%
CCTCGATTTAGCTAAATCGA+GGG + Chr7:6029362-6029343 None:intergenic 40.0%
TATAACCACATCAACAACCG+CGG + Chr7:6029420-6029439 None:intergenic 40.0%
TCCTCGATTTAGCTAAATCG+AGG + Chr7:6029346-6029365 None:intergenic 40.0%
TGACCAATCATCATCATCCA+TGG - Chr7:6029313-6029332 MsG0780036289.01.T01:CDS 40.0%
TGTGGGAACCCTAATTGTAA+GGG - Chr7:6029647-6029666 MsG0780036289.01.T01:CDS 40.0%
! CTAGTTCTTAGAGGAAAGTG+TGG - Chr7:6029851-6029870 MsG0780036289.01.T01:CDS 40.0%
! TGTTGGTAGGTTAGAAGTTC+CGG - Chr7:6029880-6029899 MsG0780036289.01.T01:CDS 40.0%
!! AGAGCTGTTCTAGTTCTTAG+AGG - Chr7:6029842-6029861 MsG0780036289.01.T01:CDS 40.0%
AGGAGCCATCTTCTTAAGCT+CGG + Chr7:6029813-6029832 None:intergenic 45.0%
AGTGTGGTTGTTCTGTTGGT+AGG - Chr7:6029867-6029886 MsG0780036289.01.T01:CDS 45.0%
AGTTCGCGATGATGATCACA+AGG + Chr7:6029785-6029804 None:intergenic 45.0%
CCCTCGATTTAGCTAAATCG+AGG - Chr7:6029342-6029361 MsG0780036289.01.T01:CDS 45.0%
TAAATCGAGGGAATTCGCCA+TGG + Chr7:6029333-6029352 None:intergenic 45.0%
! CATGTGCTGTTGTTGCTACA+TGG - Chr7:6029597-6029616 MsG0780036289.01.T01:CDS 45.0%
! GGAAAGTGTGGTTGTTCTGT+TGG - Chr7:6029863-6029882 MsG0780036289.01.T01:CDS 45.0%
! GTTGGTAGGTTAGAAGTTCC+GGG - Chr7:6029881-6029900 MsG0780036289.01.T01:CDS 45.0%
! TCGCCATGGATGATGATGAT+TGG + Chr7:6029319-6029338 None:intergenic 45.0%
CCACCATCTTTACCCAACGA+CGG + Chr7:6029734-6029753 None:intergenic 50.0%
CGAAGCCGAGCTTAAGAAGA+TGG - Chr7:6029805-6029824 MsG0780036289.01.T01:CDS 50.0%
GTGAAGAATTCGCCGTCGTT+GGG - Chr7:6029719-6029738 MsG0780036289.01.T01:CDS 50.0%
TGTGAAGAATTCGCCGTCGT+TGG - Chr7:6029718-6029737 MsG0780036289.01.T01:CDS 50.0%
! TCGTTGGGTAAAGATGGTGG+TGG - Chr7:6029734-6029753 MsG0780036289.01.T01:CDS 50.0%
CCGTCGTTGGGTAAAGATGG+TGG - Chr7:6029731-6029750 MsG0780036289.01.T01:CDS 55.0%
TCGATCCGCGGTTGTTGATG+TGG - Chr7:6029412-6029431 MsG0780036289.01.T01:CDS 55.0%
TCGCCGTCGTTGGGTAAAGA+TGG - Chr7:6029728-6029747 MsG0780036289.01.T01:CDS 55.0%
GATCGAGATCGCTCGCACTG+CGG + Chr7:6029398-6029417 None:intergenic 60.0%
GTGCGAGCGATCTCGATCCG+CGG - Chr7:6029400-6029419 MsG0780036289.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr7 gene 6029281 6029934 6029281 ID=MsG0780036289.01;Name=MsG0780036289.01
Chr7 mRNA 6029281 6029934 6029281 ID=MsG0780036289.01.T01;Parent=MsG0780036289.01;Name=MsG0780036289.01.T01;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|217
Chr7 exon 6029281 6029934 6029281 ID=MsG0780036289.01.T01:exon:747;Parent=MsG0780036289.01.T01
Chr7 CDS 6029281 6029934 6029281 ID=MsG0780036289.01.T01:cds;Parent=MsG0780036289.01.T01
Gene Sequence

>MsG0780036289.01.T01

ATGGTAAATTTCCATAGTTTGATCTCATCTGTTGACCAATCATCATCATCCATGGCGAATTCCCTCGATTTAGCTAAATCGAGGAACCGTAAGACACTCAATTCTCTTCAGATTCCGCAGTGCGAGCGATCTCGATCCGCGGTTGTTGATGTGGTTATATTTATAGCTGTTGTTATTGCTTTAGGGTTCTTGGTTTTTCCATATATTCAATTTGTGATGAGTGAAAGTTTCAAACTTTGTGGTTTGTTTATGGATTTGGTGAAAGAAGAAGTTGCTGTTGCTCCTGTGATTTATGTATCTCTTGGAGTTAGTGTTTCATGTGCTGTTGTTGCTACATGGTTTTTTATAGCTTATACATCAAGGAAATGTGGGAACCCTAATTGTAAGGGATTGAAGAATGCTGCTGAGTTTGATATTCAGTTAGAGACCGAAGATTGTGTGAAGAATTCGCCGTCGTTGGGTAAAGATGGTGGTGGAATTAAAAAGGGTCTTTTTAAGATTCCTTGTGATCATCATCGCGAACTCGAAGCCGAGCTTAAGAAGATGGCTCCTGTTAATGGAAGAGCTGTTCTAGTTCTTAGAGGAAAGTGTGGTTGTTCTGTTGGTAGGTTAGAAGTTCCGGGTCCAAAGAAGAATAGAAAGATCAAGAAATAG

Protein sequence

>MsG0780036289.01.T01

MVNFHSLISSVDQSSSSMANSLDLAKSRNRKTLNSLQIPQCERSRSAVVDVVIFIAVVIALGFLVFPYIQFVMSESFKLCGLFMDLVKEEVAVAPVIYVSLGVSVSCAVVATWFFIAYTSRKCGNPNCKGLKNAAEFDIQLETEDCVKNSPSLGKDGGGIKKGLFKIPCDHHRELEAELKKMAPVNGRAVLVLRGKCGCSVGRLEVPGPKKNRKIKK*