Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028563.01.T01 | QGL04297.1 | 66.548 | 281 | 38 | 6 | 1 | 225 | 78 | 358 | 1.19E-113 | 339 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028563.01.T01 | B0ZB57 | 40.07 | 287 | 110 | 6 | 1 | 225 | 91 | 377 | 1.41E-57 | 189 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028563.01.T01 | A0A649WZ66 | 66.548 | 281 | 38 | 6 | 1 | 225 | 78 | 358 | 5.70e-114 | 339 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000038.01 | MsG0580028563.01 | 0.821062 | 4.930755e-53 | 3.970072e-50 |
| MsG0180000114.01 | MsG0580028563.01 | 0.802994 | 4.306552e-49 | 2.139546e-46 |
| MsG0180000289.01 | MsG0580028563.01 | 0.811232 | 7.759680e-51 | 4.777275e-48 |
| MsG0180000292.01 | MsG0580028563.01 | 0.815353 | 9.655193e-52 | 6.641317e-49 |
| MsG0180000404.01 | MsG0580028563.01 | 0.819002 | 1.459538e-52 | 1.109796e-49 |
| MsG0180000785.01 | MsG0580028563.01 | 0.821932 | 3.103552e-53 | 2.560428e-50 |
| MsG0180000787.01 | MsG0580028563.01 | 0.807786 | 4.262804e-50 | 2.395938e-47 |
| MsG0180002399.01 | MsG0580028563.01 | 0.803311 | 3.704103e-49 | 1.855256e-46 |
| MsG0180003741.01 | MsG0580028563.01 | 0.810789 | 9.680747e-51 | 5.890442e-48 |
| MsG0180004246.01 | MsG0580028563.01 | 0.803147 | 4.005572e-49 | 1.997834e-46 |
| MsG0180004428.01 | MsG0580028563.01 | 0.806451 | 8.174531e-50 | 4.438467e-47 |
| MsG0180004493.01 | MsG0580028563.01 | 0.808232 | 3.426910e-50 | 1.948870e-47 |
| MsG0180004541.01 | MsG0580028563.01 | 0.811871 | 5.637095e-51 | 3.529489e-48 |
| MsG0280010736.01 | MsG0580028563.01 | 0.811590 | 6.488817e-51 | 4.032566e-48 |
| MsG0280010839.01 | MsG0580028563.01 | 0.813092 | 3.049387e-51 | 1.972574e-48 |
| MsG0280011248.01 | MsG0580028563.01 | 0.816073 | 6.674797e-52 | 4.682749e-49 |
| MsG0380014706.01 | MsG0580028563.01 | 0.811532 | 6.680517e-51 | 4.145588e-48 |
| MsG0480020832.01 | MsG0580028563.01 | 0.801873 | 7.331326e-49 | 3.540108e-46 |
| MsG0480021060.01 | MsG0580028563.01 | 0.808241 | 3.410958e-50 | 1.940338e-47 |
| MsG0480021195.01 | MsG0580028563.01 | 0.808593 | 2.869670e-50 | 1.647425e-47 |
| MsG0480023172.01 | MsG0580028563.01 | 0.806870 | 6.666543e-50 | 3.658846e-47 |
| MsG0480023488.01 | MsG0580028563.01 | 0.816090 | 6.614538e-52 | 4.642640e-49 |
| MsG0580024443.01 | MsG0580028563.01 | 0.817196 | 3.737736e-52 | 2.703968e-49 |
| MsG0580025332.01 | MsG0580028563.01 | 0.817012 | 4.110741e-52 | 2.959140e-49 |
| MsG0580025407.01 | MsG0580028563.01 | -0.830163 | 3.427947e-55 | 3.583078e-52 |
| MsG0580025607.01 | MsG0580028563.01 | 0.817510 | 3.176597e-52 | 2.318048e-49 |
| MsG0580025612.01 | MsG0580028563.01 | 0.820245 | 7.596096e-53 | 5.978221e-50 |
| MsG0580025971.01 | MsG0580028563.01 | 0.804331 | 2.273556e-49 | 1.169023e-46 |
| MsG0380015885.01 | MsG0580028563.01 | 0.804659 | 1.942552e-49 | 1.007310e-46 |
| MsG0380015896.01 | MsG0580028563.01 | 0.801679 | 8.035689e-49 | 3.860828e-46 |
| MsG0380016247.01 | MsG0580028563.01 | 0.804607 | 1.991872e-49 | 1.031491e-46 |
| MsG0380016387.01 | MsG0580028563.01 | 0.822152 | 2.760392e-53 | 2.291568e-50 |
| MsG0580028563.01 | MsG0580028960.01 | 0.803971 | 2.701787e-49 | 1.376477e-46 |
| MsG0580028563.01 | MsG0580029236.01 | 0.803675 | 3.112790e-49 | 1.573749e-46 |
| MsG0580028563.01 | MsG0580029529.01 | 0.812943 | 3.287874e-51 | 2.118376e-48 |
| MsG0580028563.01 | MsG0580029736.01 | 0.802381 | 5.764072e-49 | 2.819587e-46 |
| MsG0580028563.01 | MsG0680030908.01 | 0.822207 | 2.680102e-53 | 2.228507e-50 |
| MsG0580028563.01 | MsG0680034766.01 | 0.806186 | 9.293975e-50 | 5.012455e-47 |
| MsG0580028563.01 | MsG0680034767.01 | 0.833089 | 6.515101e-56 | 7.425607e-53 |
| MsG0580028563.01 | MsG0780036517.01 | 0.823647 | 1.237327e-53 | 1.071776e-50 |
| MsG0580028563.01 | MsG0780036593.01 | 0.810038 | 1.406121e-50 | 8.387816e-48 |
| MsG0580028563.01 | MsG0780036596.01 | 0.807462 | 4.996613e-50 | 2.784809e-47 |
| MsG0580028563.01 | MsG0780037341.01 | 0.818596 | 1.805159e-52 | 1.357065e-49 |
| MsG0580028563.01 | MsG0780037395.01 | 0.816384 | 5.685512e-52 | 4.022742e-49 |
| MsG0580028563.01 | MsG0780038378.01 | 0.812792 | 3.547410e-51 | 2.276451e-48 |
| MsG0580028563.01 | MsG0780039155.01 | 0.800589 | 1.342640e-48 | 6.275736e-46 |
| MsG0580028563.01 | MsG0780039369.01 | 0.800250 | 1.574226e-48 | 7.295064e-46 |
| MsG0580028563.01 | MsG0780040888.01 | 0.820349 | 7.190288e-53 | 5.675310e-50 |
| MsG0580028563.01 | MsG0780041074.01 | 0.821302 | 4.339419e-53 | 3.517055e-50 |
| MsG0580028563.01 | MsG0780041482.01 | 0.829252 | 5.710360e-55 | 5.813312e-52 |
| MsG0580028563.01 | MsG0880041834.01 | 0.803792 | 2.944124e-49 | 1.493017e-46 |
| MsG0580028563.01 | MsG0880041861.01 | 0.835237 | 1.885623e-56 | 2.292174e-53 |
| MsG0580028563.01 | MsG0880041864.01 | 0.805467 | 1.316322e-49 | 6.969344e-47 |
| MsG0580028563.01 | MsG0880043157.01 | 0.813187 | 2.906121e-51 | 1.884766e-48 |
| MsG0580028563.01 | MsG0880043941.01 | 0.808369 | 3.203182e-50 | 1.828291e-47 |
| MsG0580028563.01 | MsG0880044704.01 | 0.806353 | 8.571142e-50 | 4.642044e-47 |
| MsG0580028563.01 | MsG0880047696.01 | 0.812185 | 4.815518e-51 | 3.040960e-48 |
| MsG0280007432.01 | MsG0580028563.01 | 0.849789 | 2.580191e-60 | 4.947467e-57 |
| MsG0280008418.01 | MsG0580028563.01 | 0.803798 | 2.934611e-49 | 1.488440e-46 |
| MsG0280010148.01 | MsG0580028563.01 | 0.802585 | 5.231146e-49 | 2.572107e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028563.01.T01 | MTR_5g074600 | 55.674 | 282 | 68 | 7 | 1 | 225 | 78 | 359 | 9.59e-94 | 279 |
| MsG0580028563.01.T01 | MTR_5g074680 | 54.804 | 281 | 70 | 7 | 1 | 224 | 79 | 359 | 1.32e-88 | 266 |
| MsG0580028563.01.T01 | MTR_5g074540 | 53.901 | 282 | 55 | 8 | 1 | 225 | 78 | 341 | 2.52e-84 | 254 |
| MsG0580028563.01.T01 | MTR_5g074610 | 52.918 | 257 | 85 | 8 | 1 | 225 | 74 | 326 | 1.00e-80 | 244 |
| MsG0580028563.01.T01 | MTR_4g079160 | 41.901 | 284 | 101 | 7 | 1 | 225 | 77 | 355 | 1.37e-64 | 204 |
| MsG0580028563.01.T01 | MTR_5g074580 | 85.000 | 100 | 14 | 1 | 127 | 225 | 261 | 360 | 9.58e-53 | 174 |
| MsG0580028563.01.T01 | MTR_5g074580 | 66.667 | 111 | 37 | 0 | 1 | 111 | 79 | 189 | 1.30e-47 | 160 |
| MsG0580028563.01.T01 | MTR_7g014510 | 32.746 | 284 | 128 | 9 | 1 | 225 | 78 | 357 | 8.19e-34 | 124 |
| MsG0580028563.01.T01 | MTR_4g033015 | 35.192 | 287 | 123 | 7 | 1 | 225 | 78 | 363 | 2.27e-32 | 120 |
| MsG0580028563.01.T01 | MTR_4g033200 | 35.192 | 287 | 123 | 7 | 1 | 225 | 78 | 363 | 2.47e-32 | 120 |
| MsG0580028563.01.T01 | MTR_4g088190 | 59.000 | 100 | 39 | 2 | 127 | 225 | 266 | 364 | 7.84e-31 | 116 |
| MsG0580028563.01.T01 | MTR_4g088200 | 58.000 | 100 | 40 | 2 | 127 | 225 | 263 | 361 | 3.20e-30 | 115 |
| MsG0580028563.01.T01 | MTR_4g033150 | 33.798 | 287 | 127 | 7 | 1 | 225 | 78 | 363 | 1.14e-29 | 113 |
| MsG0580028563.01.T01 | MTR_4g088345 | 55.000 | 100 | 43 | 2 | 127 | 225 | 258 | 356 | 2.76e-28 | 109 |
| MsG0580028563.01.T01 | MTR_4g088340 | 55.000 | 100 | 43 | 2 | 127 | 225 | 235 | 333 | 8.06e-28 | 108 |
| MsG0580028563.01.T01 | MTR_7g024520 | 53.061 | 98 | 44 | 2 | 127 | 223 | 252 | 348 | 4.22e-27 | 106 |
| MsG0580028563.01.T01 | MTR_1g076940 | 52.000 | 100 | 45 | 3 | 127 | 225 | 264 | 361 | 1.42e-25 | 102 |
| MsG0580028563.01.T01 | MTR_7g014570 | 49.524 | 105 | 50 | 3 | 122 | 225 | 250 | 352 | 9.59e-25 | 100 |
| MsG0580028563.01.T01 | MTR_7g014570 | 34.513 | 113 | 66 | 3 | 1 | 113 | 78 | 182 | 1.47e-16 | 77.8 |
| MsG0580028563.01.T01 | MTR_7g024500 | 50.000 | 98 | 47 | 2 | 127 | 223 | 252 | 348 | 4.06e-24 | 98.6 |
| MsG0580028563.01.T01 | MTR_4g033170 | 56.566 | 99 | 43 | 0 | 127 | 225 | 259 | 357 | 5.80e-19 | 84.3 |
| MsG0580028563.01.T01 | MTR_0079s0070 | 45.545 | 101 | 49 | 4 | 127 | 225 | 238 | 334 | 3.51e-18 | 82.0 |
| MsG0580028563.01.T01 | MTR_4g038440 | 45.545 | 101 | 49 | 4 | 127 | 225 | 272 | 368 | 5.16e-18 | 82.0 |
| MsG0580028563.01.T01 | MTR_3g083620 | 43.750 | 96 | 52 | 2 | 127 | 221 | 263 | 357 | 5.23e-18 | 81.6 |
| MsG0580028563.01.T01 | MTR_0017s0100 | 44.000 | 100 | 52 | 3 | 127 | 225 | 272 | 368 | 1.69e-16 | 77.8 |
| MsG0580028563.01.T01 | MTR_1g096310 | 23.182 | 220 | 111 | 5 | 48 | 212 | 142 | 358 | 9.82e-16 | 75.5 |
| MsG0580028563.01.T01 | MTR_4g033085 | 54.545 | 99 | 45 | 0 | 127 | 225 | 259 | 357 | 2.52e-15 | 74.3 |
| MsG0580028563.01.T01 | MTR_4g025570 | 42.708 | 96 | 51 | 4 | 127 | 221 | 122 | 214 | 6.74e-15 | 71.2 |
| MsG0580028563.01.T01 | MTR_8g068730 | 44.828 | 87 | 40 | 5 | 127 | 210 | 249 | 330 | 3.00e-13 | 68.2 |
| MsG0580028563.01.T01 | MTR_1g036490 | 40.698 | 86 | 48 | 2 | 127 | 212 | 273 | 355 | 1.10e-12 | 66.6 |
| MsG0580028563.01.T01 | MTR_1g036460 | 40.698 | 86 | 48 | 2 | 127 | 212 | 273 | 355 | 1.45e-12 | 66.2 |
| MsG0580028563.01.T01 | MTR_8g068690 | 41.463 | 82 | 46 | 1 | 125 | 206 | 278 | 357 | 2.14e-12 | 65.9 |
| MsG0580028563.01.T01 | MTR_7g118320 | 44.828 | 87 | 40 | 4 | 127 | 210 | 266 | 347 | 3.97e-12 | 65.1 |
| MsG0580028563.01.T01 | MTR_8g068870 | 39.286 | 84 | 49 | 1 | 127 | 210 | 267 | 348 | 5.15e-12 | 64.7 |
| MsG0580028563.01.T01 | MTR_7g118300 | 43.820 | 89 | 44 | 4 | 127 | 214 | 279 | 362 | 5.85e-12 | 64.7 |
| MsG0580028563.01.T01 | MTR_8g024160 | 39.216 | 102 | 52 | 4 | 127 | 224 | 232 | 327 | 1.02e-11 | 63.5 |
| MsG0580028563.01.T01 | MTR_1g036510 | 38.372 | 86 | 50 | 2 | 127 | 212 | 287 | 369 | 2.57e-11 | 62.8 |
| MsG0580028563.01.T01 | MTR_0591s0010 | 46.914 | 81 | 39 | 3 | 127 | 206 | 281 | 358 | 3.23e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580028563.01.T01 | AT1G51990 | 42.157 | 102 | 52 | 4 | 127 | 225 | 265 | 362 | 2.40e-15 | 74.3 |
| MsG0580028563.01.T01 | AT1G51990 | 42.157 | 102 | 52 | 4 | 127 | 225 | 265 | 362 | 2.40e-15 | 74.3 |
| MsG0580028563.01.T01 | AT4G35160 | 40.000 | 105 | 55 | 4 | 127 | 224 | 277 | 380 | 2.00e-14 | 71.6 |
| MsG0580028563.01.T01 | AT4G35150 | 40.952 | 105 | 54 | 4 | 127 | 224 | 220 | 323 | 2.26e-14 | 71.2 |
| MsG0580028563.01.T01 | AT1G21110 | 43.678 | 87 | 46 | 2 | 122 | 207 | 271 | 355 | 1.32e-13 | 69.3 |
| MsG0580028563.01.T01 | AT1G21100 | 41.304 | 92 | 51 | 2 | 122 | 212 | 271 | 360 | 2.28e-13 | 68.6 |
| MsG0580028563.01.T01 | AT1G21130 | 41.304 | 92 | 51 | 2 | 122 | 212 | 271 | 360 | 2.55e-13 | 68.6 |
| MsG0580028563.01.T01 | AT1G21120 | 40.217 | 92 | 52 | 2 | 122 | 212 | 271 | 360 | 3.62e-12 | 65.1 |
| MsG0580028563.01.T01 | AT1G21120 | 40.217 | 92 | 52 | 2 | 122 | 212 | 319 | 408 | 3.84e-12 | 65.1 |
| MsG0580028563.01.T01 | AT1G77520 | 38.384 | 99 | 58 | 2 | 127 | 224 | 284 | 380 | 1.24e-11 | 63.5 |
| MsG0580028563.01.T01 | AT5G53810 | 38.384 | 99 | 58 | 2 | 127 | 224 | 281 | 377 | 7.22e-11 | 61.6 |
Find 70 sgRNAs with CRISPR-Local
Find 110 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATTCAGTCACACAATATT+TGG | 0.159363 | 5:+82455484 | MsG0580028563.01.T01:three_prime_UTR |
| GTTAAATGAGTTGTTAAATT+TGG | 0.170908 | 5:-82454458 | None:intergenic |
| ATAATTAATTGAATGTATTT+TGG | 0.181853 | 5:+82455571 | MsG0580028563.01.T01:three_prime_UTR |
| CAACACATGGGATGTCGTTT+TGG | 0.213169 | 5:+82454416 | MsG0580028563.01.T01:CDS |
| ATAGACCTTGATCCTAAAAT+TGG | 0.254900 | 5:-82455242 | None:intergenic |
| TTTGTCTTAAGAGGATGATT+TGG | 0.260141 | 5:+82455451 | MsG0580028563.01.T01:three_prime_UTR |
| CTCGATTAGTTTCCGATTGT+TGG | 0.304828 | 5:+82454500 | MsG0580028563.01.T01:CDS |
| TGCCATGGATCTATCATATC+TGG | 0.330412 | 5:-82454346 | None:intergenic |
| ATGCGAATCATGACTTATTC+CGG | 0.334688 | 5:+82454196 | None:intergenic |
| TACAGTCGATATTGCATGAT+TGG | 0.335498 | 5:+82454976 | MsG0580028563.01.T01:intron |
| TTGTCTTAAGAGGATGATTT+GGG | 0.336204 | 5:+82455452 | MsG0580028563.01.T01:three_prime_UTR |
| TTTGGACATACGCACTTAAT+CGG | 0.352771 | 5:+82455502 | MsG0580028563.01.T01:three_prime_UTR |
| AGACAAGGAAAATACTTATA+TGG | 0.355177 | 5:-82455636 | None:intergenic |
| CAACCAATGACTCAACTTCA+TGG | 0.372843 | 5:-82454545 | None:intergenic |
| AGAGATTCATTCAGTGACTA+TGG | 0.373815 | 5:+82455821 | MsG0580028563.01.T01:three_prime_UTR |
| ACAATGCAGAGGGATGGTTT+TGG | 0.380712 | 5:-82455420 | None:intergenic |
| AAGAGAGAAACGAGAAAGAA+TGG | 0.424421 | 5:+82455174 | MsG0580028563.01.T01:CDS |
| ATGGATTAAGTTGATTTCCT+TGG | 0.431482 | 5:+82455193 | MsG0580028563.01.T01:CDS |
| GTTCCATGAAGTTGAGTCAT+TGG | 0.432542 | 5:+82454542 | MsG0580028563.01.T01:CDS |
| CAACTCATTTAACGAATCAA+TGG | 0.437712 | 5:+82454468 | MsG0580028563.01.T01:CDS |
| GCAATATCAAAGAAAGGTAA+AGG | 0.460727 | 5:+82455041 | MsG0580028563.01.T01:CDS |
| CTTATATGGTCACAATAGCT+TGG | 0.463243 | 5:-82455622 | None:intergenic |
| TGCACTTCTCAACCAACAAT+CGG | 0.465918 | 5:-82454512 | None:intergenic |
| TTCTTCCTCATCCACGGTAG+AGG | 0.467948 | 5:+82455373 | MsG0580028563.01.T01:three_prime_UTR |
| CATGGCATCAATTCTCTGCT+TGG | 0.468036 | 5:+82454362 | MsG0580028563.01.T01:CDS |
| GTTGAGTCATTGGTTGATGT+TGG | 0.468409 | 5:+82454552 | MsG0580028563.01.T01:CDS |
| ATTAAGTTGATTTCCTTGGC+TGG | 0.468848 | 5:+82455197 | MsG0580028563.01.T01:CDS |
| CCAAATATTGTGTGACTGAA+TGG | 0.482917 | 5:-82455484 | None:intergenic |
| CAACATAACTAAAATTGAAA+AGG | 0.492599 | 5:-82455964 | None:intergenic |
| GCACTTAATCGGAAAATAAG+TGG | 0.499566 | 5:+82455513 | MsG0580028563.01.T01:three_prime_UTR |
| AAAGATATTAAAGAATTGTA+AGG | 0.505831 | 5:+82455016 | MsG0580028563.01.T01:CDS |
| TTATATGGTCACAATAGCTT+GGG | 0.511873 | 5:-82455621 | None:intergenic |
| GGAATAGAGATGTAGAGAAA+CGG | 0.514416 | 5:-82455350 | None:intergenic |
| AACATTGTGATGATGACAAA+TGG | 0.517874 | 5:-82455305 | None:intergenic |
| TAACGAATACAACAGTAAAA+CGG | 0.525035 | 5:-82455766 | None:intergenic |
| ACATGTGGTGCCACACTCAT+TGG | 0.525895 | 5:-82454307 | None:intergenic |
| CAATATCAAAGAAAGGTAAA+GGG | 0.532814 | 5:+82455042 | MsG0580028563.01.T01:CDS |
| TCACTCCTCTACCGTGGATG+AGG | 0.542925 | 5:-82455378 | None:intergenic |
| TTGATGATGGTAGTATTCGC+AGG | 0.543043 | 5:+82455149 | MsG0580028563.01.T01:CDS |
| AAGGATGCAATATCAAAGAA+AGG | 0.545671 | 5:+82455035 | MsG0580028563.01.T01:CDS |
| TCATCCTCTTAAGACAAAAG+AGG | 0.545757 | 5:-82455446 | None:intergenic |
| CATCCTCTTAAGACAAAAGA+GGG | 0.549685 | 5:-82455445 | None:intergenic |
| CTTCTATGACATGTTGATGA+TGG | 0.553245 | 5:+82455136 | MsG0580028563.01.T01:CDS |
| TCTACCGTGGATGAGGAAGA+AGG | 0.556802 | 5:-82455371 | None:intergenic |
| TATTCCTTCTTCCTCATCCA+CGG | 0.561235 | 5:+82455367 | MsG0580028563.01.T01:three_prime_UTR |
| ACGGTGTTAGACAAAGAGAA+TGG | 0.563271 | 5:+82455083 | MsG0580028563.01.T01:CDS |
| GAGTCATTGGTTGATGTTGG+AGG | 0.570761 | 5:+82454555 | MsG0580028563.01.T01:CDS |
| AAAGAAAGGTAAAGGGAAGG+TGG | 0.580286 | 5:+82455049 | MsG0580028563.01.T01:CDS |
| CCACGGTAGAGGAGTGATTG+TGG | 0.581604 | 5:+82455384 | MsG0580028563.01.T01:three_prime_UTR |
| CAAGCAGAGAATTGATGCCA+TGG | 0.591666 | 5:-82454361 | None:intergenic |
| CTGAGTATCCCGTGCACATG+TGG | 0.594919 | 5:-82454322 | None:intergenic |
| GTAGTCACTAAAACCAGCCA+AGG | 0.595683 | 5:-82455210 | None:intergenic |
| GTCCAGATATGATAGATCCA+TGG | 0.611089 | 5:+82454344 | MsG0580028563.01.T01:CDS |
| ATTGTTGGTTGAGAAGTGCA+AGG | 0.617450 | 5:+82454515 | MsG0580028563.01.T01:CDS |
| ATCAAAGAAAGGTAAAGGGA+AGG | 0.618639 | 5:+82455046 | MsG0580028563.01.T01:CDS |
| TCATTGGTTGATGTTGGAGG+AGG | 0.622074 | 5:+82454558 | MsG0580028563.01.T01:CDS |
| TCTTAAGAGGATGATTTGGG+TGG | 0.624562 | 5:+82455455 | MsG0580028563.01.T01:three_prime_UTR |
| TTGTTGGTTGAGAAGTGCAA+GGG | 0.627382 | 5:+82454516 | MsG0580028563.01.T01:CDS |
| AGGGAGACAATGCAGAGGGA+TGG | 0.628535 | 5:-82455426 | None:intergenic |
| GAAAATCACTTATTTAGACA+AGG | 0.642671 | 5:-82455651 | None:intergenic |
| GTGTGGCACCACATGTGCAC+GGG | 0.643351 | 5:+82454314 | MsG0580028563.01.T01:CDS |
| ACAGCATTTGAAACAACACA+TGG | 0.644519 | 5:+82454403 | MsG0580028563.01.T01:CDS |
| AGTGTGGCACCACATGTGCA+CGG | 0.654606 | 5:+82454313 | MsG0580028563.01.T01:CDS |
| AAAGAGGGAGACAATGCAGA+GGG | 0.658598 | 5:-82455430 | None:intergenic |
| AAAAGAGGGAGACAATGCAG+AGG | 0.667854 | 5:-82455431 | None:intergenic |
| TAAGAACAGTCCAATGAGTG+TGG | 0.672166 | 5:+82454297 | MsG0580028563.01.T01:CDS |
| CCACAATCACTCCTCTACCG+TGG | 0.683105 | 5:-82455384 | None:intergenic |
| GAAGGTGGTTATCATTGACA+CGG | 0.700974 | 5:+82455064 | MsG0580028563.01.T01:CDS |
| ATTGCATGATTGGAATGACG+AGG | 0.708926 | 5:+82454986 | MsG0580028563.01.T01:CDS |
| CAGCATTTGAAACAACACAT+GGG | 0.716263 | 5:+82454404 | MsG0580028563.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAATTAATTGAATGTATTT+TGG | + | Chr5:82455571-82455590 | MsG0580028563.01.T01:three_prime_UTR | 10.0% |
| !!! | TAATTTGATTTTTTTTTTCT+TGG | + | Chr5:82454798-82454817 | MsG0580028563.01.T01:intron | 10.0% |
| !! | AAAGATATTAAAGAATTGTA+AGG | + | Chr5:82455016-82455035 | MsG0580028563.01.T01:CDS | 15.0% |
| !! | AAGGAAAAATATTATCTAAT+TGG | - | Chr5:82454878-82454897 | None:intergenic | 15.0% |
| !!! | ATATTAGTTTACACATTATA+GGG | + | Chr5:82454774-82454793 | MsG0580028563.01.T01:intron | 15.0% |
| !!! | TATATTAGTTTACACATTAT+AGG | + | Chr5:82454773-82454792 | MsG0580028563.01.T01:intron | 15.0% |
| !!! | TTTGAATTTTTCATATTTGT+AGG | + | Chr5:82455885-82455904 | MsG0580028563.01.T01:three_prime_UTR | 15.0% |
| !! | CAAATATGAAAAATTCAAAG+TGG | - | Chr5:82455884-82455903 | None:intergenic | 20.0% |
| !! | CAACATAACTAAAATTGAAA+AGG | - | Chr5:82455967-82455986 | None:intergenic | 20.0% |
| !! | GTTAAATGAGTTGTTAAATT+TGG | - | Chr5:82454461-82454480 | None:intergenic | 20.0% |
| !!! | ATTTTATAGATTATGGATCA+TGG | - | Chr5:82455281-82455300 | None:intergenic | 20.0% |
| !!! | CAATGTTCTTTTATTCAAAT+TGG | - | Chr5:82456017-82456036 | None:intergenic | 20.0% |
| ! | AGACAAGGAAAATACTTATA+TGG | - | Chr5:82455639-82455658 | None:intergenic | 25.0% |
| ! | AGTGAAAACTTAAACTATGT+TGG | + | Chr5:82454675-82454694 | MsG0580028563.01.T01:intron | 25.0% |
| ! | AGTGGAAAAAATAGAAAACA+AGG | - | Chr5:82454897-82454916 | None:intergenic | 25.0% |
| ! | CAATATCAAAGAAAGGTAAA+GGG | + | Chr5:82455042-82455061 | MsG0580028563.01.T01:CDS | 25.0% |
| ! | GAAAATCACTTATTTAGACA+AGG | - | Chr5:82455654-82455673 | None:intergenic | 25.0% |
| ! | TAACGAATACAACAGTAAAA+CGG | - | Chr5:82455769-82455788 | None:intergenic | 25.0% |
| !! | CCATATTTTTCTACTAATGA+CGG | + | Chr5:82454852-82454871 | MsG0580028563.01.T01:intron | 25.0% |
| !! | TACAAGATTACTCCAATTTT+AGG | + | Chr5:82455230-82455249 | MsG0580028563.01.T01:CDS | 25.0% |
| !! | TGGCTAGATTTTATAGATTA+TGG | - | Chr5:82455288-82455307 | None:intergenic | 25.0% |
| !!! | TTTTTTCTTGGTACAAAAGA+GGG | + | Chr5:82454810-82454829 | MsG0580028563.01.T01:intron | 25.0% |
| !!! | TTTTTTTCTTGGTACAAAAG+AGG | + | Chr5:82454809-82454828 | MsG0580028563.01.T01:intron | 25.0% |
| AAGGATGCAATATCAAAGAA+AGG | + | Chr5:82455035-82455054 | MsG0580028563.01.T01:CDS | 30.0% | |
| ATAGACCTTGATCCTAAAAT+TGG | - | Chr5:82455245-82455264 | None:intergenic | 30.0% | |
| CAACTCATTTAACGAATCAA+TGG | + | Chr5:82454468-82454487 | MsG0580028563.01.T01:CDS | 30.0% | |
| CCGTCATTAGTAGAAAAATA+TGG | - | Chr5:82454855-82454874 | None:intergenic | 30.0% | |
| GAAAACTTAAACTATGTTGG+TGG | + | Chr5:82454678-82454697 | MsG0580028563.01.T01:intron | 30.0% | |
| GAAAAGAGAACAAACTAAAG+TGG | - | Chr5:82454915-82454934 | None:intergenic | 30.0% | |
| GCAATATCAAAGAAAGGTAA+AGG | + | Chr5:82455041-82455060 | MsG0580028563.01.T01:CDS | 30.0% | |
| TATGAAAAATTCAAAGTGGC+TGG | - | Chr5:82455880-82455899 | None:intergenic | 30.0% | |
| TTATATGGTCACAATAGCTT+GGG | - | Chr5:82455624-82455643 | None:intergenic | 30.0% | |
| TTCAACAAAATTGCATCTGT+TGG | - | Chr5:82454726-82454745 | None:intergenic | 30.0% | |
| TTGTCTTAAGAGGATGATTT+GGG | + | Chr5:82455452-82455471 | MsG0580028563.01.T01:three_prime_UTR | 30.0% | |
| TTTGTCTTAAGAGGATGATT+TGG | + | Chr5:82455451-82455470 | MsG0580028563.01.T01:three_prime_UTR | 30.0% | |
| ! | AACAGATGCAATTTTGTTGA+AGG | + | Chr5:82454725-82454744 | MsG0580028563.01.T01:intron | 30.0% |
| ! | AACATTGTGATGATGACAAA+TGG | - | Chr5:82455308-82455327 | None:intergenic | 30.0% |
| ! | TATGTTTGAGTTACGAAAGA+AGG | - | Chr5:82454943-82454962 | None:intergenic | 30.0% |
| ! | TTTTTCTTGGTACAAAAGAG+GGG | + | Chr5:82454811-82454830 | MsG0580028563.01.T01:intron | 30.0% |
| !! | ATGGATTAAGTTGATTTCCT+TGG | + | Chr5:82455193-82455212 | MsG0580028563.01.T01:CDS | 30.0% |
| !!! | TTACTCCAATTTTAGGATCA+AGG | + | Chr5:82455237-82455256 | MsG0580028563.01.T01:CDS | 30.0% |
| AACAAAATTGCATCTGTTGG+CGG | - | Chr5:82454723-82454742 | None:intergenic | 35.0% | |
| AACACAATGCATTCCATTTG+TGG | - | Chr5:82454621-82454640 | None:intergenic | 35.0% | |
| AAGAGAGAAACGAGAAAGAA+TGG | + | Chr5:82455174-82455193 | MsG0580028563.01.T01:CDS | 35.0% | |
| ACACAATGCATTCCATTTGT+GGG | - | Chr5:82454620-82454639 | None:intergenic | 35.0% | |
| AGAGATTCATTCAGTGACTA+TGG | + | Chr5:82455821-82455840 | MsG0580028563.01.T01:three_prime_UTR | 35.0% | |
| ATCAAAGAAAGGTAAAGGGA+AGG | + | Chr5:82455046-82455065 | MsG0580028563.01.T01:CDS | 35.0% | |
| CATCCTCTTAAGACAAAAGA+GGG | - | Chr5:82455448-82455467 | None:intergenic | 35.0% | |
| CCAAATATTGTGTGACTGAA+TGG | - | Chr5:82455487-82455506 | None:intergenic | 35.0% | |
| CCATTCAGTCACACAATATT+TGG | + | Chr5:82455484-82455503 | MsG0580028563.01.T01:three_prime_UTR | 35.0% | |
| CTTATATGGTCACAATAGCT+TGG | - | Chr5:82455625-82455644 | None:intergenic | 35.0% | |
| GCACTTAATCGGAAAATAAG+TGG | + | Chr5:82455513-82455532 | MsG0580028563.01.T01:three_prime_UTR | 35.0% | |
| GGAATAGAGATGTAGAGAAA+CGG | - | Chr5:82455353-82455372 | None:intergenic | 35.0% | |
| TACAGTCGATATTGCATGAT+TGG | + | Chr5:82454976-82454995 | MsG0580028563.01.T01:intron | 35.0% | |
| TCATCCTCTTAAGACAAAAG+AGG | - | Chr5:82455449-82455468 | None:intergenic | 35.0% | |
| TTCCATTTGTGGGAATGATT+TGG | - | Chr5:82454610-82454629 | None:intergenic | 35.0% | |
| TTTGGACATACGCACTTAAT+CGG | + | Chr5:82455502-82455521 | MsG0580028563.01.T01:three_prime_UTR | 35.0% | |
| ! | ACAGCATTTGAAACAACACA+TGG | + | Chr5:82454403-82454422 | MsG0580028563.01.T01:CDS | 35.0% |
| ! | ATTTTGTTGAGAGAAGAAGC+CGG | - | Chr5:82454218-82454237 | None:intergenic | 35.0% |
| ! | CAGCATTTGAAACAACACAT+GGG | + | Chr5:82454404-82454423 | MsG0580028563.01.T01:CDS | 35.0% |
| ! | CTTCTATGACATGTTGATGA+TGG | + | Chr5:82455136-82455155 | MsG0580028563.01.T01:CDS | 35.0% |
| !! | ATTAAGTTGATTTCCTTGGC+TGG | + | Chr5:82455197-82455216 | MsG0580028563.01.T01:CDS | 35.0% |
| AAAGAAAGGTAAAGGGAAGG+TGG | + | Chr5:82455049-82455068 | MsG0580028563.01.T01:CDS | 40.0% | |
| ATTGCATGATTGGAATGACG+AGG | + | Chr5:82454986-82455005 | MsG0580028563.01.T01:CDS | 40.0% | |
| CAACCAATGACTCAACTTCA+TGG | - | Chr5:82454548-82454567 | None:intergenic | 40.0% | |
| GAAGGTGGTTATCATTGACA+CGG | + | Chr5:82455064-82455083 | MsG0580028563.01.T01:CDS | 40.0% | |
| GCAAACCATCAACAACATGT+GGG | - | Chr5:82454650-82454669 | None:intergenic | 40.0% | |
| GTCCAGATATGATAGATCCA+TGG | + | Chr5:82454344-82454363 | MsG0580028563.01.T01:CDS | 40.0% | |
| GTTCCATGAAGTTGAGTCAT+TGG | + | Chr5:82454542-82454561 | MsG0580028563.01.T01:CDS | 40.0% | |
| TAAGAACAGTCCAATGAGTG+TGG | + | Chr5:82454297-82454316 | MsG0580028563.01.T01:CDS | 40.0% | |
| TATTCCTTCTTCCTCATCCA+CGG | + | Chr5:82455367-82455386 | MsG0580028563.01.T01:three_prime_UTR | 40.0% | |
| TGCAAACCATCAACAACATG+TGG | - | Chr5:82454651-82454670 | None:intergenic | 40.0% | |
| TGCACTTCTCAACCAACAAT+CGG | - | Chr5:82454515-82454534 | None:intergenic | 40.0% | |
| TGCCAAATCATTCCCACAAA+TGG | + | Chr5:82454605-82454624 | MsG0580028563.01.T01:intron | 40.0% | |
| TGCCATGGATCTATCATATC+TGG | - | Chr5:82454349-82454368 | None:intergenic | 40.0% | |
| ! | ACGGTGTTAGACAAAGAGAA+TGG | + | Chr5:82455083-82455102 | MsG0580028563.01.T01:CDS | 40.0% |
| ! | ATTGTTGGTTGAGAAGTGCA+AGG | + | Chr5:82454515-82454534 | MsG0580028563.01.T01:CDS | 40.0% |
| ! | GTTGAGTCATTGGTTGATGT+TGG | + | Chr5:82454552-82454571 | MsG0580028563.01.T01:CDS | 40.0% |
| ! | TCTCCCTCTTTTGTCTTAAG+AGG | + | Chr5:82455442-82455461 | MsG0580028563.01.T01:three_prime_UTR | 40.0% |
| ! | TCTTAAGAGGATGATTTGGG+TGG | + | Chr5:82455455-82455474 | MsG0580028563.01.T01:three_prime_UTR | 40.0% |
| ! | TTGTTGGTTGAGAAGTGCAA+GGG | + | Chr5:82454516-82454535 | MsG0580028563.01.T01:CDS | 40.0% |
| ! | TTTGGCAAGAGTTTTTGCCA+TGG | - | Chr5:82454592-82454611 | None:intergenic | 40.0% |
| !! | CATGTTGTTGATGGTTTGCA+AGG | + | Chr5:82454651-82454670 | MsG0580028563.01.T01:intron | 40.0% |
| !! | CTCGATTAGTTTCCGATTGT+TGG | + | Chr5:82454500-82454519 | MsG0580028563.01.T01:CDS | 40.0% |
| !! | TTGATGATGGTAGTATTCGC+AGG | + | Chr5:82455149-82455168 | MsG0580028563.01.T01:CDS | 40.0% |
| !!! | AACACATGGGATGTCGTTTT+GGG | + | Chr5:82454417-82454436 | MsG0580028563.01.T01:CDS | 40.0% |
| AAAAGAGGGAGACAATGCAG+AGG | - | Chr5:82455434-82455453 | None:intergenic | 45.0% | |
| AAAGAGGGAGACAATGCAGA+GGG | - | Chr5:82455433-82455452 | None:intergenic | 45.0% | |
| CAAGCAGAGAATTGATGCCA+TGG | - | Chr5:82454364-82454383 | None:intergenic | 45.0% | |
| CATGGCATCAATTCTCTGCT+TGG | + | Chr5:82454362-82454381 | MsG0580028563.01.T01:CDS | 45.0% | |
| GATCTCCCACATGTTGTTGA+TGG | + | Chr5:82454642-82454661 | MsG0580028563.01.T01:intron | 45.0% | |
| GTAGTCACTAAAACCAGCCA+AGG | - | Chr5:82455213-82455232 | None:intergenic | 45.0% | |
| ! | ACAATGCAGAGGGATGGTTT+TGG | - | Chr5:82455423-82455442 | None:intergenic | 45.0% |
| ! | GAGTCATTGGTTGATGTTGG+AGG | + | Chr5:82454555-82454574 | MsG0580028563.01.T01:CDS | 45.0% |
| ! | TCATTGGTTGATGTTGGAGG+AGG | + | Chr5:82454558-82454577 | MsG0580028563.01.T01:CDS | 45.0% |
| !! | CAACACATGGGATGTCGTTT+TGG | + | Chr5:82454416-82454435 | MsG0580028563.01.T01:CDS | 45.0% |
| TCTACCGTGGATGAGGAAGA+AGG | - | Chr5:82455374-82455393 | None:intergenic | 50.0% | |
| TTCTTCCTCATCCACGGTAG+AGG | + | Chr5:82455373-82455392 | MsG0580028563.01.T01:three_prime_UTR | 50.0% | |
| ! | GGTTGATGTTGGAGGAGGTA+CGG | + | Chr5:82454563-82454582 | MsG0580028563.01.T01:intron | 50.0% |
| ! | GTTGATGTTGGAGGAGGTAC+GGG | + | Chr5:82454564-82454583 | MsG0580028563.01.T01:intron | 50.0% |
| ! | TTGATGTTGGAGGAGGTACG+GGG | + | Chr5:82454565-82454584 | MsG0580028563.01.T01:intron | 50.0% |
| !! | ACATGTGGTGCCACACTCAT+TGG | - | Chr5:82454310-82454329 | None:intergenic | 50.0% |
| AGGGAGACAATGCAGAGGGA+TGG | - | Chr5:82455429-82455448 | None:intergenic | 55.0% | |
| AGTGTGGCACCACATGTGCA+CGG | + | Chr5:82454313-82454332 | MsG0580028563.01.T01:CDS | 55.0% | |
| CCACAATCACTCCTCTACCG+TGG | - | Chr5:82455387-82455406 | None:intergenic | 55.0% | |
| CCACGGTAGAGGAGTGATTG+TGG | + | Chr5:82455384-82455403 | MsG0580028563.01.T01:three_prime_UTR | 55.0% | |
| CTGAGTATCCCGTGCACATG+TGG | - | Chr5:82454325-82454344 | None:intergenic | 55.0% | |
| TCACTCCTCTACCGTGGATG+AGG | - | Chr5:82455381-82455400 | None:intergenic | 55.0% | |
| GTGTGGCACCACATGTGCAC+GGG | + | Chr5:82454314-82454333 | MsG0580028563.01.T01:CDS | 60.0% | |
| ! | TGGAGGAGGTACGGGGACCA+TGG | + | Chr5:82454572-82454591 | MsG0580028563.01.T01:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 82454205 | 82456039 | 82454205 | ID=MsG0580028563.01;Name=MsG0580028563.01 |
| Chr5 | mRNA | 82454205 | 82456039 | 82454205 | ID=MsG0580028563.01.T01;Parent=MsG0580028563.01;Name=MsG0580028563.01.T01;_AED=0.47;_eAED=0.47;_QI=0|0|0.5|1|1|1|2|756|225 |
| Chr5 | exon | 82454205 | 82454579 | 82454205 | ID=MsG0580028563.01.T01:exon:21197;Parent=MsG0580028563.01.T01 |
| Chr5 | exon | 82454981 | 82456039 | 82454981 | ID=MsG0580028563.01.T01:exon:21198;Parent=MsG0580028563.01.T01 |
| Chr5 | CDS | 82454205 | 82454579 | 82454205 | ID=MsG0580028563.01.T01:cds;Parent=MsG0580028563.01.T01 |
| Chr5 | CDS | 82454981 | 82455283 | 82454981 | ID=MsG0580028563.01.T01:cds;Parent=MsG0580028563.01.T01 |
| Chr5 | three_prime_UTR | 82455284 | 82456039 | 82455284 | ID=MsG0580028563.01.T01:three_prime_utr;Parent=MsG0580028563.01.T01 |
| Gene Sequence |
| Protein sequence |
