AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880041995.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880041995.01.T01 MTR_8g009073 80.741 135 26 0 1 135 100 234 1.38e-75 232
MsG0880041995.01.T01 MTR_1g019510 81.481 135 25 0 1 135 100 234 9.82e-74 227
MsG0880041995.01.T01 MTR_8g008970 75.000 132 33 0 1 132 100 231 1.20e-66 215
MsG0880041995.01.T01 MTR_8g008970 59.574 47 19 0 89 135 527 573 9.00e-13 64.3
MsG0880041995.01.T01 MTR_8g009067 71.970 132 37 0 1 132 100 231 7.45e-64 202
MsG0880041995.01.T01 MTR_8g009063 65.926 135 46 0 1 135 107 241 4.72e-63 200
MsG0880041995.01.T01 MTR_8g009073 80.000 105 21 0 31 135 1 105 5.11e-58 183
MsG0880041995.01.T01 MTR_8g008990 62.963 135 50 0 1 135 100 234 1.51e-57 185
MsG0880041995.01.T01 MTR_8g009055 59.259 135 55 0 1 135 100 234 4.36e-55 179
MsG0880041995.01.T01 MTR_8g009067 70.796 113 33 0 20 132 1 113 2.58e-54 174
MsG0880041995.01.T01 MTR_8g009050 59.259 135 55 0 1 135 100 234 1.82e-53 174
MsG0880041995.01.T01 MTR_0078s0010 50.382 131 65 0 1 131 100 230 5.22e-40 139
MsG0880041995.01.T01 MTR_7g047030 50.382 131 65 0 1 131 100 230 1.88e-39 138
MsG0880041995.01.T01 MTR_7g047270 50.382 131 65 0 1 131 100 230 1.88e-39 138
MsG0880041995.01.T01 MTR_0078s0200 49.587 121 61 0 9 129 111 231 4.96e-38 131
MsG0880041995.01.T01 MTR_0078s0060 50.746 134 66 0 1 134 100 233 2.70e-37 132
MsG0880041995.01.T01 MTR_0184s0040 48.092 131 68 0 1 131 115 245 2.83e-37 132
MsG0880041995.01.T01 MTR_0078s0190 48.092 131 68 0 1 131 115 245 2.83e-37 132
MsG0880041995.01.T01 MTR_0184s0030 49.587 121 61 0 9 129 111 231 1.24e-36 130
MsG0880041995.01.T01 MTR_8g006260 46.032 126 68 0 1 126 103 228 4.64e-36 129
MsG0880041995.01.T01 MTR_7g117170 41.935 124 72 0 10 133 118 241 1.44e-34 125
MsG0880041995.01.T01 MTR_8g006270 45.968 124 67 0 5 128 101 224 2.01e-31 116
MsG0880041995.01.T01 MTR_8g077590 45.736 129 69 1 5 132 107 235 2.12e-31 116
MsG0880041995.01.T01 MTR_8g006230 40.000 125 75 0 11 135 53 177 1.65e-29 108
MsG0880041995.01.T01 MTR_8g027870 41.739 115 64 1 12 126 121 232 2.06e-27 105
MsG0880041995.01.T01 MTR_8g027885 41.739 115 64 1 12 126 121 232 5.63e-27 104
MsG0880041995.01.T01 MTR_8g012745 42.857 119 67 1 8 125 108 226 1.46e-26 103
MsG0880041995.01.T01 MTR_6g005660 40.441 136 80 1 1 135 101 236 2.35e-26 99.8
MsG0880041995.01.T01 MTR_8g012650 40.945 127 74 1 3 128 103 229 4.63e-26 102
MsG0880041995.01.T01 MTR_8g012635 43.443 122 68 1 8 128 108 229 2.39e-25 99.8
MsG0880041995.01.T01 MTR_6g005600 39.706 136 81 1 1 135 101 236 9.64e-25 98.2
MsG0880041995.01.T01 MTR_7g046490 45.312 128 70 0 1 128 100 227 1.70e-23 94.7
MsG0880041995.01.T01 MTR_8g012630 49.367 79 39 1 5 82 106 184 6.25e-19 81.6
MsG0880041995.01.T01 MTR_8g074550 38.168 131 78 2 1 131 103 230 8.42e-19 81.6
MsG0880041995.01.T01 MTR_8g006710 33.083 133 84 3 1 128 98 230 1.60e-13 66.6
MsG0880041995.01.T01 MTR_8g465670 32.520 123 81 2 7 127 109 231 2.54e-13 65.9
MsG0880041995.01.T01 MTR_8g006220 32.520 123 81 2 7 127 108 230 3.13e-13 65.5
MsG0880041995.01.T01 MTR_6g033660 28.125 128 91 1 1 127 106 233 3.30e-13 65.5
MsG0880041995.01.T01 MTR_8g006730 30.827 133 87 3 1 128 99 231 5.65e-13 64.7
MsG0880041995.01.T01 MTR_6g023665 30.769 130 87 3 1 127 107 236 9.47e-13 64.3
MsG0880041995.01.T01 MTR_6g488280 31.538 130 86 3 1 127 100 229 3.34e-12 62.8
MsG0880041995.01.T01 MTR_0428s0010 31.538 130 86 3 1 127 100 229 3.34e-12 62.8
MsG0880041995.01.T01 MTR_6g033725 25.984 127 92 1 1 125 103 229 1.90e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880041995.01.T01 AT4G01070 41.600 125 73 0 11 135 112 236 2.06e-33 120
MsG0880041995.01.T01 AT4G01070 41.600 125 73 0 11 135 112 236 1.56e-32 119
MsG0880041995.01.T01 AT1G01390 40.650 123 73 0 13 135 114 236 3.30e-30 113
MsG0880041995.01.T01 AT1G01390 40.650 123 73 0 13 135 131 253 3.42e-30 113
MsG0880041995.01.T01 AT1G01420 39.535 129 77 1 7 135 112 239 9.96e-30 112
MsG0880041995.01.T01 AT1G01420 39.535 129 77 1 7 135 109 236 1.05e-29 112
MsG0880041995.01.T01 AT5G66690 35.088 114 74 0 12 125 107 220 3.06e-15 71.2
MsG0880041995.01.T01 AT2G18570 34.109 129 77 4 4 128 103 227 4.57e-15 70.9
MsG0880041995.01.T01 AT2G18560 31.034 116 80 0 13 128 22 137 6.98e-14 67.4
MsG0880041995.01.T01 AT3G50740 28.448 116 83 0 12 127 112 227 3.64e-13 65.5
MsG0880041995.01.T01 AT5G26310 30.000 130 84 1 5 127 93 222 2.47e-12 63.2

Find 25 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATGAAATCCTTAACTTTA+AGG 0.150458 8:-2286184 MsG0880041995.01.T01:CDS
TGCAATTGAAGCATTAAATT+TGG 0.158797 8:-2286129 MsG0880041995.01.T01:CDS
TCTTCTGTGAGTGCTTCTAT+AGG 0.359525 8:+2285822 None:intergenic
AAAAGGTGTCCTTAAAGTTA+AGG 0.363066 8:+2286175 None:intergenic
GGTCCAACAGCTTAAACATC+AGG 0.423805 8:+2285786 None:intergenic
CCAGAGCCTCTTAAAGTACC+AGG 0.432347 8:-2285990 MsG0880041995.01.T01:CDS
ACACAACCTGGTACTTTAAG+AGG 0.438976 8:+2285984 None:intergenic
GACAAAGTTGTGACTGAAGC+AGG 0.441595 8:+2286071 None:intergenic
GACTTGATCTATCTTGAGCT+TGG 0.458150 8:+2285934 None:intergenic
CCTGGTACTTTAAGAGGCTC+TGG 0.459752 8:+2285990 None:intergenic
GAACATTCCGTTCTGTTGAC+GGG 0.460484 8:-2285881 MsG0880041995.01.T01:CDS
TGAAGCATTAAATTTGGCTA+AGG 0.482987 8:-2286123 MsG0880041995.01.T01:CDS
ATAGAAGCACTCACAGAAGA+AGG 0.498098 8:-2285819 MsG0880041995.01.T01:CDS
TTGTGTACTGATTCACGGTC+GGG 0.500136 8:-2285967 MsG0880041995.01.T01:CDS
AGAACATTCCGTTCTGTTGA+CGG 0.503976 8:-2285882 MsG0880041995.01.T01:CDS
TCAACCACCAAGGCCACAAA+AGG 0.509857 8:+2286158 None:intergenic
CCAGGTTGTGTACTGATTCA+CGG 0.553171 8:-2285972 MsG0880041995.01.T01:CDS
GCACTCACAGAAGAAGGAAG+TGG 0.564801 8:-2285813 MsG0880041995.01.T01:CDS
CTATATGCTCAAATATGACA+AGG 0.580217 8:-2286039 MsG0880041995.01.T01:CDS
GTTGTGTACTGATTCACGGT+CGG 0.587026 8:-2285968 MsG0880041995.01.T01:CDS
CCGTGAATCAGTACACAACC+TGG 0.589117 8:+2285972 None:intergenic
GTCCAACAGCTTAAACATCA+GGG 0.605783 8:+2285787 None:intergenic
TAAGAATCCCGTCAACAGAA+CGG 0.627171 8:+2285874 None:intergenic
TGAAGCAGGAAAGTAAACAT+AGG 0.627209 8:+2286085 None:intergenic
TGCAAAAGAATCAACCACCA+AGG 0.684027 8:+2286148 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGCTTTTTAGAAATTGAAAA+AGG - Chr8:2286133-2286152 MsG0880041995.01.T01:CDS 20.0%
!! TGCAATTGAAGCATTAAATT+TGG - Chr8:2285850-2285869 MsG0880041995.01.T01:CDS 25.0%
CTATATGCTCAAATATGACA+AGG - Chr8:2285940-2285959 MsG0880041995.01.T01:CDS 30.0%
! TGAAGCATTAAATTTGGCTA+AGG - Chr8:2285856-2285875 MsG0880041995.01.T01:CDS 30.0%
!! AAAAGGTGTCCTTAAAGTTA+AGG + Chr8:2285807-2285826 None:intergenic 30.0%
TGAAGCAGGAAAGTAAACAT+AGG + Chr8:2285897-2285916 None:intergenic 35.0%
!! AACTTTAAGGACACCTTTTG+TGG - Chr8:2285808-2285827 MsG0880041995.01.T01:CDS 35.0%
ACACAACCTGGTACTTTAAG+AGG + Chr8:2285998-2286017 None:intergenic 40.0%
AGAACATTCCGTTCTGTTGA+CGG - Chr8:2286097-2286116 MsG0880041995.01.T01:CDS 40.0%
GACTTGATCTATCTTGAGCT+TGG + Chr8:2286048-2286067 None:intergenic 40.0%
TAAGAATCCCGTCAACAGAA+CGG + Chr8:2286108-2286127 None:intergenic 40.0%
TCTTCTGTGAGTGCTTCTAT+AGG + Chr8:2286160-2286179 None:intergenic 40.0%
TGCAAAAGAATCAACCACCA+AGG + Chr8:2285834-2285853 None:intergenic 40.0%
!! ATAGAAGCACTCACAGAAGA+AGG - Chr8:2286160-2286179 MsG0880041995.01.T01:CDS 40.0%
GAACATTCCGTTCTGTTGAC+GGG - Chr8:2286098-2286117 MsG0880041995.01.T01:CDS 45.0%
GACAAAGTTGTGACTGAAGC+AGG + Chr8:2285911-2285930 None:intergenic 45.0%
!! CCAGGTTGTGTACTGATTCA+CGG - Chr8:2286007-2286026 MsG0880041995.01.T01:CDS 45.0%
!! GTTGTGTACTGATTCACGGT+CGG - Chr8:2286011-2286030 MsG0880041995.01.T01:CDS 45.0%
!! TTGTGTACTGATTCACGGTC+GGG - Chr8:2286012-2286031 MsG0880041995.01.T01:CDS 45.0%
CCGTGAATCAGTACACAACC+TGG + Chr8:2286010-2286029 None:intergenic 50.0%
GCACTCACAGAAGAAGGAAG+TGG - Chr8:2286166-2286185 MsG0880041995.01.T01:CDS 50.0%
TCAACCACCAAGGCCACAAA+AGG + Chr8:2285824-2285843 None:intergenic 50.0%
! AAGGACACCTTTTGTGGCCT+TGG - Chr8:2285814-2285833 MsG0880041995.01.T01:CDS 50.0%
! CCAGAGCCTCTTAAAGTACC+AGG - Chr8:2285989-2286008 MsG0880041995.01.T01:CDS 50.0%
! CCTGGTACTTTAAGAGGCTC+TGG + Chr8:2285992-2286011 None:intergenic 50.0%
!! GACACCTTTTGTGGCCTTGG+TGG - Chr8:2285817-2285836 MsG0880041995.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 2285797 2286204 2285797 ID=MsG0880041995.01;Name=MsG0880041995.01
Chr8 mRNA 2285797 2286204 2285797 ID=MsG0880041995.01.T01;Parent=MsG0880041995.01;Name=MsG0880041995.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|135
Chr8 exon 2285797 2286204 2285797 ID=MsG0880041995.01.T01:exon:1786;Parent=MsG0880041995.01.T01
Chr8 CDS 2285797 2286204 2285797 ID=MsG0880041995.01.T01:cds;Parent=MsG0880041995.01.T01
Gene Sequence

>MsG0880041995.01.T01

ATGAAATCCTTAACTTTAAGGACACCTTTTGTGGCCTTGGTGGTTGATTCTTTTGCAATTGAAGCATTAAATTTGGCTAAGGAATTCAACATGTTGTCCTATGTTTACTTTCCTGCTTCAGTCACAACTTTGTCTTCATACTTCTATATGCTCAAATATGACAAGGAAACATCATGTGAGTACAGAGATTTTCCAGAGCCTCTTAAAGTACCAGGTTGTGTACTGATTCACGGTCGGGATCTTGTTGACCAAGCTCAAGATAGATCAAGTCAATCTTACAAATTTTTACTCGAACGCATTAGAACATTCCGTTCTGTTGACGGGATTCTTATTAATAGCTTTTTAGAAATTGAAAAAGGTCCTATAGAAGCACTCACAGAAGAAGGAAGTGGCAACCCTGATGTTTAA

Protein sequence

>MsG0880041995.01.T01

MKSLTLRTPFVALVVDSFAIEALNLAKEFNMLSYVYFPASVTTLSSYFYMLKYDKETSCEYRDFPEPLKVPGCVLIHGRDLVDQAQDRSSQSYKFLLERIRTFRSVDGILINSFLEIEKGPIEALTEEGSGNPDV*