AlfalfaGEDB | Msa0037720

M. sativa cultivar zhongmu-4 / Msa0037720

KEGG: K00799
GO: -
PFAM: PF02798.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0037720	RDX73629.1	61.187	438	154	2	7	444	1	422	0.0	570

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0037720	sp\|Q03662\|GSTX1_TOBAC	49.296	213	105	2	225	437	1	210	9.88e-61	200
Msa0037720	sp\|Q03662\|GSTX1_TOBAC	46.226	212	108	4	7	217	1	207	2.23e-54	184

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0011920	Msa0037720	-0.832612	8.560479e-56	-8.615850e-47
Msa0030050	Msa0037720	0.859916	2.969950e-63	-8.615850e-47
Msa0037280	Msa0037720	0.836515	8.943039e-57	-8.615850e-47
Msa0037650	Msa0037720	-0.808143	3.579636e-50	-8.615850e-47
Msa0037720	Msa0039890	0.826610	2.467960e-54	-8.615850e-47
Msa0037720	Msa0052780	0.836601	8.502461e-57	-8.615850e-47
Msa0037720	Msa0074860	-0.807890	4.052581e-50	-8.615850e-47
Msa0037720	Msa0076640	0.811447	6.967919e-51	-8.615850e-47
Msa0037720	Msa0081700	-0.810864	9.322373e-51	-8.615850e-47
Msa0037720	Msa0083240	0.957072	6.545906e-115	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0037720	MtrunA17_Chr1g0196661	91.556	225	19	0	225	449	1	225	9.56e-152	430
Msa0037720	MtrunA17_Chr1g0196661	77.578	223	49	1	7	229	1	222	2.53e-126	365
Msa0037720	MtrunA17_Chr1g0196691	94.009	217	13	0	1	217	20	236	2.30e-149	424
Msa0037720	MtrunA17_Chr1g0196691	79.730	222	44	1	224	444	25	246	9.59e-129	372
Msa0037720	MtrunA17_Chr1g0196721	87.442	215	27	0	228	442	3	217	1.11e-135	389
Msa0037720	MtrunA17_Chr1g0196721	80.288	208	40	1	10	217	3	209	1.67e-120	350
Msa0037720	MtrunA17_Chr1g0196711	81.778	225	41	0	225	449	1	225	4.21e-133	382
Msa0037720	MtrunA17_Chr1g0196711	74.439	223	56	1	7	229	1	222	6.07e-119	346
Msa0037720	MtrunA17_Chr4g0029341	69.302	215	66	0	227	441	4	218	1.44e-108	320
Msa0037720	MtrunA17_Chr4g0029341	64.115	209	74	1	9	217	4	211	4.32e-96	288

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0037720	AT2G29420.1	43.056	216	121	1	227	442	9	222	2.03e-54	181
Msa0037720	AT2G29420.1	37.387	222	133	3	9	230	9	224	4.31e-45	157
Msa0037720	AT3G09270.1	45.205	219	116	3	228	444	7	223	2.23e-54	181
Msa0037720	AT3G09270.1	46.190	210	106	6	10	216	7	212	1.40e-49	169
Msa0037720	AT2G29460.1	39.726	219	129	2	227	444	7	223	1.49e-52	176
Msa0037720	AT2G29460.1	36.652	221	136	4	6	225	4	221	7.81e-44	153
Msa0037720	AT2G29470.1	40.455	220	127	3	228	445	8	225	2.45e-52	176
Msa0037720	AT2G29470.1	39.189	222	128	5	10	228	8	225	6.73e-50	169
Msa0037720	AT2G29450.1	42.291	227	121	4	223	444	2	223	8.41e-52	174
Msa0037720	AT2G29450.1	39.545	220	126	4	9	225	6	221	1.09e-46	161

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Find 104 sgRNAs with CRISPR-Local

Find 470 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTCCAGGGATAATTTAT+TGG	0.296970	1_1:-66843893	Msa0037720:CDS
AACACAAGCTAGGATGGCAG+AGG	0.663463	1_1:-66838020	Msa0037720:CDS
AACATATTTAACCCAAAATC+GGG	0.426033	1_1:+66837730	None:intergenic
AACATTGTAGACATAGCTCT+TGG	0.450264	1_1:-66836886	Msa0037720:CDS
AACATTGTAGACATAGCTCT+TGG	0.450264	1_1:-66843802	Msa0037720:CDS
AATGATCAGAATAAGTTCTA+TGG	0.275936	1_1:-66843838	Msa0037720:CDS
AATGATCATGGACTCACATA+TGG	0.394155	1_1:+66837823	None:intergenic
AATGTATTCAACAATAATCA+TGG	0.478690	1_1:+66844915	None:intergenic
AATGTATTCAACAATGATCA+TGG	0.484642	1_1:+66837811	None:intergenic
AATTGGTGAAGAGCGAGAGA+AGG	0.634346	1_1:-66836995	Msa0037720:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGATAAAACTACATGGATCT+TGG	-	chr1_1:66836418-66836437	Msa0037720:three_prime_UTR	30.0%
TATACCATATGACAGTATAG+AGG	-	chr1_1:66836488-66836507	Msa0037720:three_prime_UTR	30.0%
GGATTATATTGAAGAAGTTG+AGG	+	chr1_1:66836534-66836553	None:intergenic	30.0%
ATAATCATGGACTCACATAT+AGG	+	chr1_1:66836597-66836616	None:intergenic	30.0%
GAACATGTATCATGATAGAA+AGG	+	chr1_1:66836782-66836801	None:intergenic	30.0%
AAAGTTAAGTCACTAGTGTA+AGG	+	chr1_1:66836895-66836914	None:intergenic	30.0%
TGATAACAAGATCACAATCT+TGG	-	chr1_1:66836998-66837017	Msa0037720:CDS	30.0%
TTAGAAGTCTTACTCTTGAT+TGG	+	chr1_1:66837349-66837368	None:intergenic	30.0%
TTCCATCGAAGCATAATTAA+AGG	-	chr1_1:66837594-66837613	Msa0037720:intron	30.0%
AAAGTCCAGGGATAATTTAT+TGG	-	chr1_1:66837629-66837648	Msa0037720:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	66836393	66845151	66836393	ID=Msa0037720;Name=Msa0037720
chr1_1	mRNA	66836393	66845151	66836393	ID=Msa0037720-mRNA-1;Parent=Msa0037720;Name=Msa0037720-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0\|0.33\|0.25\|1\|0.66\|0.5\|4\|288\|449
chr1_1	exon	66836393	66837049	66836393	ID=Msa0037720-mRNA-1:exon:21648;Parent=Msa0037720-mRNA-1
chr1_1	exon	66837721	66838050	66837721	ID=Msa0037720-mRNA-1:exon:21647;Parent=Msa0037720-mRNA-1
chr1_1	exon	66843645	66843968	66843645	ID=Msa0037720-mRNA-1:exon:21646;Parent=Msa0037720-mRNA-1
chr1_1	exon	66844825	66845151	66844825	ID=Msa0037720-mRNA-1:exon:21645;Parent=Msa0037720-mRNA-1
chr1_1	CDS	66844825	66845151	66844825	ID=Msa0037720-mRNA-1:cds;Parent=Msa0037720-mRNA-1
chr1_1	CDS	66843645	66843968	66843645	ID=Msa0037720-mRNA-1:cds;Parent=Msa0037720-mRNA-1
chr1_1	CDS	66837721	66838050	66837721	ID=Msa0037720-mRNA-1:cds;Parent=Msa0037720-mRNA-1
chr1_1	CDS	66836681	66837049	66836681	ID=Msa0037720-mRNA-1:cds;Parent=Msa0037720-mRNA-1
chr1_1	three_prime_UTR	66836393	66836680	66836393	ID=Msa0037720-mRNA-1:three_prime_utr;Parent=Msa0037720-mRNA-1

Gene Sequence

>Msa0037720

ATGCACAAGCTAATTAAGATGGCAGAGATAAAACTACATGGATCTTGGTATAGCCCTTTCACTTTAAGGGTGGTATGGACTCTAAATCTGAAGGGTATACCATATGACAGTATAGAGGAAGATCACTTCAATAGGACTCCTCAACTTCTTCAATATAATCCAATACACAAGAAGACTCCAGTGCTGGTTCATGATGGAAAACCTATATGTGAGTCCATGATTATTGTTGAATACATTGATGAGATATGGCCACACAACTCATTGCTTCCTGCTGATCCATATGACAGAGCTCAAGCAAGATTTTGGGTTAAATATGTTGATGACATGTTTCCGGCAATTGTGGCGTTCCATCGAAGCATAATTAAAGGTGAAGAGCGGGAAAAGTCCAGGGATAATTTATTGGAGCGACTCGGAGTTGTTGAGGATCAGTGCTTGAATGATCAGAATAAGTTCTATGGGGGAGAAACCATTAACATTGTAGACATAGCTCTTGGATCTTTTGTGAAATTTATTGAGGTTCAAGAAGATATGTTTGAAGTGAAGATCTTGAAAAGTGAGAGATTCCCCCGCTTGCATTTATGGTTTAATAATTTCAAGGATGTTCCAGCCATCAAAGAAAATACCTCTGAGCAAGAGAAGTTTGTGGCTTATGATAACACAACACAAGCTAGGATGGCAGAGGTGAAACTACATGGATTTTGGTATAGTCCTTTTACTTTAAGGGTAGTATGGACCTTAAATTTGAAGGGTATATCATATGAGAACATAGAGGAAGATCGCTTCAATAAAAGTCCTCTGCTTCTTCAATATAACCCAATACACAAGAAAACTCCAGTGCTAGTTCATGATGGAAAACCCATATGTGAGTCCATGATCATTGTTGAATACATTGATGAGATGTGGTCACACAACTCATTGGTTCCTGTTGATCCCTATGACAGAGCTCAAGCCCGATTTTGGGTTAAATATGTTGATGAATTGATTTCGGCAATTGCGGCACTCCTCTATAGCAAAATTGGTGAAGAGCGAGAGAAGGCCAGAGAGAATTTATGGGAGCGACTCAGAGTTATTGAAGATAAGTGTTTGGATGATCAAAAGAAATTTTATGGGGGAGACACCATTAACATTGTAGACATAGCTCTTGGATCTTTTATCAAATTTATTGAGGTTCAAGAAGATATGTTTGAAGTGAAGATCTTGCAAAGTGAGAGATTCCCTCGATTGCATTTATGGTTTAATAATTTTAAGGATGTTCCCGTCATCAAAGAAAACACCCCTGACCAAGAGAAGTTGGTGGCCTTTCTTAATTCTCTAATTGAAAAAGTCAAGGCAGTCAAGGCATCTTCTTGA

Protein sequence

>Msa0037720

MHKLIKMAEIKLHGSWYSPFTLRVVWTLNLKGIPYDSIEEDHFNRTPQLLQYNPIHKKTPVLVHDGKPICESMIIVEYIDEIWPHNSLLPADPYDRAQARFWVKYVDDMFPAIVAFHRSIIKGEEREKSRDNLLERLGVVEDQCLNDQNKFYGGETINIVDIALGSFVKFIEVQEDMFEVKILKSERFPRLHLWFNNFKDVPAIKENTSEQEKFVAYDNTTQARMAEVKLHGFWYSPFTLRVVWTLNLKGISYENIEEDRFNKSPLLLQYNPIHKKTPVLVHDGKPICESMIIVEYIDEMWSHNSLVPVDPYDRAQARFWVKYVDELISAIAALLYSKIGEEREKARENLWERLRVIEDKCLDDQKKFYGGDTINIVDIALGSFIKFIEVQEDMFEVKILQSERFPRLHLWFNNFKDVPVIKENTPDQEKLVAFLNSLIEKVKAVKASS*