AlfalfaGEDB | Msa1254870

M. sativa cultivar zhongmu-4 / Msa1254870

KEGG: -
GO: -
PFAM: PF00561.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1254870	XP_013446375.1	96.562	320	11	0	2	321	1	320	0.0	642

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1254870	sp\|I6YGS0\|EPHA_MYCTU	36.778	329	188	7	4	320	1	321	4.72e-63	205

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1254870	A0A072TSN0	96.562	320	11	0	2	321	1	320	0.0	642

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000610	Msa1254870	-0.805819	1.110622e-49	-8.615850e-47
Msa0000800	Msa1254870	0.891462	3.887563e-74	-8.615850e-47
Msa0001510	Msa1254870	-0.813514	2.462664e-51	-8.615850e-47
Msa0002110	Msa1254870	-0.807836	4.161149e-50	-8.615850e-47
Msa0002300	Msa1254870	0.800249	1.574663e-48	-8.615850e-47
Msa0002690	Msa1254870	-0.847579	1.057908e-59	-8.615850e-47
Msa0002700	Msa1254870	-0.850628	1.501225e-60	-8.615850e-47
Msa0004180	Msa1254870	-0.868521	6.138124e-66	-8.615850e-47
Msa0004940	Msa1254870	0.850872	1.282110e-60	-8.615850e-47
Msa0004990	Msa1254870	0.836632	8.353816e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1254870	MtrunA17_Chr8g0372491	96.562	320	11	0	2	321	1	320	0.0	642
Msa1254870	MtrunA17_Chr7g0272441	47.368	323	158	4	4	318	1	319	1.09e-108	319
Msa1254870	MtrunA17_Chr7g0272501	47.988	323	156	4	4	318	1	319	2.33e-108	318
Msa1254870	MtrunA17_Chr4g0067581	47.468	316	162	3	7	318	40	355	7.86e-108	318
Msa1254870	MtrunA17_Chr7g0272431	46.440	323	161	5	4	318	1	319	1.53e-107	316
Msa1254870	MtrunA17_Chr7g0272531	48.276	319	161	3	4	318	31	349	1.98e-107	317
Msa1254870	MtrunA17_Chr7g0272171	47.649	319	163	3	4	318	1	319	1.30e-106	313
Msa1254870	MtrunA17_Chr7g0272451	47.188	320	160	5	4	318	1	316	2.24e-106	313
Msa1254870	MtrunA17_Chr7g0272481	46.708	319	166	3	4	318	1	319	4.75e-106	312
Msa1254870	MtrunA17_Chr7g0269471	46.688	317	167	2	4	318	1	317	1.48e-105	311

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1254870	AT3G51000.1	63.009	319	113	4	5	319	3	320	4.55e-152	429
Msa1254870	AT4G02340.1	47.812	320	158	5	4	318	1	316	4.76e-107	315
Msa1254870	AT3G05600.1	46.341	328	159	6	4	318	1	324	1.99e-100	299
Msa1254870	AT2G26740.1	47.664	321	159	5	7	318	1	321	3.92e-91	275
Msa1254870	AT4G15960.1	41.745	321	178	4	7	318	54	374	4.10e-88	269
Msa1254870	AT2G26750.1	46.372	317	162	5	7	315	1	317	2.16e-87	265
Msa1254870	AT4G15955.3	41.325	317	148	8	8	311	6	297	8.85e-81	248
Msa1254870	AT3G05600.2	46.559	247	117	5	4	239	1	243	4.08e-74	228
Msa1254870	AT4G15955.2	50.289	173	75	3	8	169	6	178	1.82e-56	181
Msa1254870	AT4G15955.1	50.303	165	71	3	8	161	6	170	2.51e-53	173

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Find 88 sgRNAs with CRISPR-Local

Find 332 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCAACATCTTTGTCTC+CGG	0.451884	8_2:-53198573	None:intergenic
AAAGCTCTCTCTGCACGACC+TGG	0.595328	8_2:-53198196	None:intergenic
AAATCAACAATCTCCATGCC+AGG	0.548033	8_2:-53198292	None:intergenic
AAGGGTAATCTACCTGAAAT+TGG	0.307281	8_2:-53198096	None:intergenic
AATATACATGTAGCAGAACA+AGG	0.633512	8_2:+53196739	Msa1254870:CDS
AATGATCAAGAAGACCAATA+AGG	0.475165	8_2:-53196942	None:intergenic
ACAAACCCCTTCACTCTATC+AGG	0.370013	8_2:-53197983	None:intergenic
ACGATACACAACAACTTATT+AGG	0.240318	8_2:+53196661	None:intergenic
ACTCAATGGTTACAGAAGCT+GGG	0.603841	8_2:+53198496	Msa1254870:intron
ACTGGGGAGCAAACATTGGA+TGG	0.572887	8_2:+53197942	Msa1254870:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATATACATGTAGCAGAACA+AGG	+	chr8_2:53196739-53196758	Msa1254870:CDS	30.0%
TACACTCTTCAACATATTGT+TGG	+	chr8_2:53196916-53196935	Msa1254870:CDS	30.0%
AATGATCAAGAAGACCAATA+AGG	-	chr8_2:53196945-53196964	None:intergenic	30.0%
TTGTGGTCAATTCAACTATA+AGG	+	chr8_2:53197165-53197184	Msa1254870:intron	30.0%
AAACGATTTACCATAGAGTT+CGG	+	chr8_2:53197430-53197449	Msa1254870:intron	30.0%
GAGTCATATTCAACAACTTT+AGG	+	chr8_2:53197603-53197622	Msa1254870:intron	30.0%
TATATGTAAACTTGAGGTCT+TGG	-	chr8_2:53197759-53197778	None:intergenic	30.0%
TTAGGAAAAAGGATAGTTTG+AGG	-	chr8_2:53197866-53197885	None:intergenic	30.0%
TCTTTATCCCATTTGAATGA+AGG	+	chr8_2:53197901-53197920	Msa1254870:intron	30.0%
ACATATGCCTTCATTCAAAT+GGG	-	chr8_2:53197911-53197930	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	53196672	53198919	53196672	ID=Msa1254870;Name=Msa1254870
chr8_2	mRNA	53196672	53198919	53196672	ID=Msa1254870-mRNA-1;Parent=Msa1254870;Name=Msa1254870-mRNA-1;_AED=0.11;_eAED=0.11;_QI=16\|1\|1\|1\|1\|1\|4\|159\|321
chr8_2	exon	53196672	53196978	53196672	ID=Msa1254870-mRNA-1:exon:16419;Parent=Msa1254870-mRNA-1
chr8_2	exon	53197923	53198105	53197923	ID=Msa1254870-mRNA-1:exon:16420;Parent=Msa1254870-mRNA-1
chr8_2	exon	53198193	53198434	53198193	ID=Msa1254870-mRNA-1:exon:16421;Parent=Msa1254870-mRNA-1
chr8_2	exon	53198511	53198919	53198511	ID=Msa1254870-mRNA-1:exon:16422;Parent=Msa1254870-mRNA-1
chr8_2	five_prime_UTR	53196672	53196687	53196672	ID=Msa1254870-mRNA-1:five_prime_utr;Parent=Msa1254870-mRNA-1
chr8_2	CDS	53196688	53196978	53196688	ID=Msa1254870-mRNA-1:cds;Parent=Msa1254870-mRNA-1
chr8_2	CDS	53197923	53198105	53197923	ID=Msa1254870-mRNA-1:cds;Parent=Msa1254870-mRNA-1
chr8_2	CDS	53198193	53198434	53198193	ID=Msa1254870-mRNA-1:cds;Parent=Msa1254870-mRNA-1
chr8_2	CDS	53198511	53198760	53198511	ID=Msa1254870-mRNA-1:cds;Parent=Msa1254870-mRNA-1
chr8_2	three_prime_UTR	53198761	53198919	53198761	ID=Msa1254870-mRNA-1:three_prime_utr;Parent=Msa1254870-mRNA-1

Gene Sequence

>Msa1254870

ATGTTCTTCATGACTGAAGTGAATCACCAAAGAATCAAAACCAACGGAATAAATATACATGTAGCAGAACAAGGAACAGGACCACTAGTTCTACTGCTTCATGGTTTCCCAGAAATATGGTATTCTTGGCGCCACCAACTCAACTACTTGGCCCAAAATGGTTATCATGCTGTTGCACCTGATCTAAGAGGATATGGTGACTCCGATTCTCCTATCAACCCCAATTCATACACTCTTCAACATATTGTTGGTGACCTTATTGGTCTTCTTGATCATTTTGGTGAACAAAAGGCATATGTAGTAGGATCTGACTGGGGAGCAAACATTGGATGGCACCTAAGTCTATTTAGGCCTGATAGAGTGAAGGGGTTTGTTGCTCTATGTGTTCCTTACTTTCCAAGATCTCCAACAGATAAAACTGTTGAAACTATAAGAAAAGTATACGGAGATGGAGCTCATGTTTGCCAATTTCAGGAACCAGGTCGTGCAGAGAGAGCTTTTGCTAGATATGATTGTTTGACAGTGATGAAGAAGTTTTTGCTGATTACATGGACACATTTTATAACAGCTCCTCCTGGCATGGAGATTGTTGATTTCTTGCCAACTCCTTCGGTTTTGCCGTCGTGGATAACCGAGGAAGAACTCATGGTTTTTGCAGACAAATTCCAAGAGTCTGGTTTCACCGGTGCATTCAATTACTATCGCGCAATGGACCTAAGCTGGGAACTTCTAGCACCATGGCAAGGATCAAAGATAACAGTTCCAACTAAGTTTATTGCCGGAGACAAAGATGTTGGTTTTCAAAATGGGGGTACAAAAGATTTTGTGGAAGGTGATATTTTCCAGAGTCTTGTTCCTAACCTTGAAGTGGTTATACTAGATGGTCACCACTATATACATCAAGAGAAAGCTCAACAAGTTTCGGAGGAAATCCTTTCCTTCATCCGTAAACTATCTCTAGATTAG

Protein sequence

>Msa1254870

MFFMTEVNHQRIKTNGINIHVAEQGTGPLVLLLHGFPEIWYSWRHQLNYLAQNGYHAVAPDLRGYGDSDSPINPNSYTLQHIVGDLIGLLDHFGEQKAYVVGSDWGANIGWHLSLFRPDRVKGFVALCVPYFPRSPTDKTVETIRKVYGDGAHVCQFQEPGRAERAFARYDCLTVMKKFLLITWTHFITAPPGMEIVDFLPTPSVLPSWITEEELMVFADKFQESGFTGAFNYYRAMDLSWELLAPWQGSKITVPTKFIAGDKDVGFQNGGTKDFVEGDIFQSLVPNLEVVILDGHHYIHQEKAQQVSEEILSFIRKLSLD*