AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1366480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1366480 MtrunA17_Chr7g0269511 96.814 565 18 0 1 565 1 565 0.0 1132
Msa1366480 MtrunA17_Chr7g0269521 67.228 534 167 4 36 565 34 563 0.0 733
Msa1366480 MtrunA17_Chr1g0175661 64.685 555 179 5 14 565 19 559 0.0 732
Msa1366480 MtrunA17_Chr1g0195301 66.604 533 173 3 36 565 26 556 0.0 725
Msa1366480 MtrunA17_Chr1g0195321 66.604 533 173 3 36 565 26 556 0.0 725
Msa1366480 MtrunA17_Chr1g0195331 63.908 568 190 4 1 565 1 556 0.0 723
Msa1366480 MtrunA17_Chr1g0195351 63.556 568 190 5 1 565 1 554 0.0 716
Msa1366480 MtrunA17_Chr7g0269531 62.172 534 196 5 34 564 35 565 0.0 677
Msa1366480 MtrunA17_Chr1g0192331 52.562 527 218 6 37 560 27 524 0.0 535
Msa1366480 MtrunA17_Chr3g0080381 50.545 550 240 11 40 566 90 630 5.12e-180 521
Msa1366480 MtrunA17_Chr8g0354911 50.462 541 246 8 41 564 77 612 1.78e-178 517
Msa1366480 MtrunA17_Chr1g0195311 76.779 267 62 0 299 565 49 315 1.32e-153 442
Msa1366480 MtrunA17_Chr7g0269541 48.780 492 224 8 72 560 86 552 2.37e-148 438
Msa1366480 MtrunA17_Chr4g0011771 45.251 537 225 18 42 564 32 513 2.98e-132 395
Msa1366480 MtrunA17_Chr8g0391311 44.068 531 266 11 41 564 35 541 5.12e-132 395
Msa1366480 MtrunA17_Chr8g0391291 44.068 531 266 11 41 564 33 539 2.30e-131 394
Msa1366480 MtrunA17_Chr1g0147051 45.492 488 232 9 93 564 138 607 2.48e-131 396
Msa1366480 MtrunA17_Chr4g0011791 45.180 529 232 14 42 564 33 509 8.90e-131 391
Msa1366480 MtrunA17_Chr4g0069881 40.426 564 280 12 14 564 15 535 3.77e-128 385
Msa1366480 MtrunA17_Chr2g0302021 46.055 469 213 10 101 564 92 525 8.21e-128 384
Msa1366480 MtrunA17_Chr8g0345281 42.964 533 283 8 40 564 70 589 3.54e-127 385
Msa1366480 MtrunA17_Chr3g0126771 41.368 585 288 15 9 564 39 597 3.26e-126 383
Msa1366480 MtrunA17_Chr8g0354901 44.946 465 227 8 100 564 146 581 8.76e-124 376
Msa1366480 MtrunA17_Chr3g0080391 43.871 465 232 8 100 564 139 574 6.68e-123 373
Msa1366480 MtrunA17_Chr4g0069841 44.540 467 220 8 101 564 86 516 6.98e-123 371
Msa1366480 MtrunA17_Chr7g0272141 43.856 472 226 10 101 566 106 544 1.48e-122 372
Msa1366480 MtrunA17_Chr7g0233831 44.754 467 222 10 101 564 122 555 7.48e-122 370
Msa1366480 MtrunA17_Chr4g0069861 43.368 475 218 8 101 564 83 517 3.17e-121 367
Msa1366480 MtrunA17_Chr4g0011751 42.827 467 226 8 93 558 57 483 4.50e-120 363
Msa1366480 MtrunA17_Chr7g0233771 44.540 467 223 10 101 564 121 554 3.07e-119 363
Msa1366480 MtrunA17_Chr7g0233801 42.348 477 240 9 90 564 110 553 1.88e-114 351
Msa1366480 MtrunA17_Chr4g0018371 38.961 539 274 12 42 564 40 539 9.56e-113 346
Msa1366480 MtrunA17_Chr3g0090091 38.534 532 279 11 42 564 36 528 4.86e-111 341
Msa1366480 MtrunA17_Chr4g0069891 40.830 458 255 5 101 558 146 587 4.29e-110 341
Msa1366480 MtrunA17_Chr3g0079361 42.366 465 225 10 104 564 102 527 6.48e-110 338
Msa1366480 MtrunA17_Chr3g0079341 42.366 465 225 10 104 564 102 527 2.85e-109 337
Msa1366480 MtrunA17_Chr3g0079351 37.925 530 284 11 42 564 36 527 1.81e-108 335
Msa1366480 MtrunA17_Chr2g0302001 51.186 295 141 1 270 564 146 437 5.42e-106 325
Msa1366480 MtrunA17_Chr8g0345211 38.505 535 288 15 40 564 79 582 7.15e-103 322
Msa1366480 MtrunA17_Chr7g0270691 35.214 585 324 17 1 565 1 550 3.01e-100 314
Msa1366480 MtrunA17_Chr1g0194091 38.125 480 255 7 101 560 108 565 8.59e-98 308
Msa1366480 MtrunA17_Chr1g0192321 37.838 481 265 11 98 561 117 580 1.36e-97 308
Msa1366480 MtrunA17_Chr1g0194081 35.581 534 307 9 40 560 50 559 7.95e-97 306
Msa1366480 MtrunA17_Chr1g0192311 37.849 465 262 8 101 560 124 566 6.15e-94 298
Msa1366480 MtrunA17_Chr1g0192281 37.849 465 262 8 101 560 124 566 6.15e-94 298
Msa1366480 MtrunA17_Chr2g0301931 51.408 284 134 2 283 566 15 294 1.44e-93 288
Msa1366480 MtrunA17_Chr3g0129921 35.345 580 297 19 11 559 27 559 2.17e-93 296
Msa1366480 MtrunA17_Chr7g0272151 33.095 559 294 9 42 561 63 580 3.68e-93 297
Msa1366480 MtrunA17_Chr8g0384121 34.687 591 297 18 8 559 19 559 1.13e-92 295
Msa1366480 MtrunA17_Chr4g0000121 36.933 463 269 7 101 560 111 553 3.80e-90 288
Msa1366480 MtrunA17_Chr1g0192301 37.149 463 268 7 101 560 129 571 7.37e-90 288
Msa1366480 MtrunA17_Chr4g0067611 35.669 471 271 8 100 560 120 568 9.48e-88 282
Msa1366480 MtrunA17_Chr4g0067601 35.095 473 273 7 100 561 120 569 2.44e-86 279
Msa1366480 MtrunA17_Chr7g0234711 55.411 231 101 2 336 564 39 269 1.31e-85 266
Msa1366480 MtrunA17_Chr2g0295531 33.568 569 323 15 5 561 18 543 3.31e-84 274
Msa1366480 MtrunA17_Chr7g0270651 34.511 481 282 6 98 564 105 566 7.93e-83 269
Msa1366480 MtrunA17_Chr7g0270661 35.306 490 266 8 101 564 108 572 1.69e-81 266
Msa1366480 MtrunA17_Chr7g0270671 35.306 490 266 8 101 564 108 572 1.69e-81 266
Msa1366480 MtrunA17_Chr2g0295551 32.451 567 338 12 11 561 24 561 2.40e-81 265
Msa1366480 MtrunA17_Chr8g0380581 40.419 334 187 5 230 561 50 373 1.51e-78 252
Msa1366480 MtrunA17_Chr7g0270631 34.082 490 274 9 98 565 93 555 4.31e-77 264
Msa1366480 MtrunA17_Chr7g0270631 32.041 490 294 8 98 564 641 1114 3.80e-68 239
Msa1366480 MtrunA17_Chr5g0437831 41.424 309 175 4 254 560 75 379 3.74e-75 243
Msa1366480 MtrunA17_Chr7g0271381 31.016 561 333 15 12 560 34 552 1.60e-72 242
Msa1366480 MtrunA17_Chr7g0268241 40.635 315 171 6 252 564 50 350 1.69e-71 233
Msa1366480 MtrunA17_Chr2g0334111 40.317 315 170 7 252 564 25 323 3.24e-71 231
Msa1366480 MtrunA17_Chr2g0295541 30.948 559 321 11 42 561 60 592 6.96e-68 230
Msa1366480 MtrunA17_Chr6g0487101 39.016 305 158 7 260 553 93 380 4.28e-57 196
Msa1366480 MtrunA17_Chr8g0389771 38.111 307 160 7 260 553 88 377 1.21e-55 192
Msa1366480 MtrunA17_Chr6g0453551 34.591 318 179 7 251 555 5 306 2.46e-53 184
Msa1366480 MtrunA17_Chr1g0194311 34.936 312 176 8 252 553 74 368 2.94e-50 177
Msa1366480 MtrunA17_Chr7g0217941 34.375 320 177 10 251 555 5 306 5.51e-48 169
Msa1366480 MtrunA17_Chr6g0466961 34.579 321 177 11 241 553 29 324 1.27e-47 169
Msa1366480 MtrunA17_Chr8g0391261 54.015 137 62 1 429 564 30 166 7.02e-47 161
Msa1366480 MtrunA17_Chr7g0258341 33.333 312 184 7 252 553 90 387 1.85e-46 167
Msa1366480 MtrunA17_Chr4g0070051 31.402 328 192 10 236 553 39 343 4.23e-45 162
Msa1366480 MtrunA17_Chr6g0466951 30.938 320 190 10 241 553 58 353 1.25e-42 156
Msa1366480 MtrunA17_Chr5g0446301 33.117 308 177 7 258 553 67 357 2.43e-41 152
Msa1366480 MtrunA17_Chr7g0265201 31.494 308 182 7 253 553 100 385 5.81e-39 147
Msa1366480 MtrunA17_Chr5g0399221 29.245 318 196 7 251 556 62 362 6.21e-39 146
Msa1366480 MtrunA17_Chr1g0161731 34.035 285 162 7 252 527 48 315 1.50e-38 144
Msa1366480 MtrunA17_Chr2g0294321 27.879 330 209 7 234 554 38 347 1.37e-37 142
Msa1366480 MtrunA17_Chr4g0037751 31.068 309 190 8 251 553 70 361 1.30e-35 137
Msa1366480 MtrunA17_Chr5g0440871 31.922 307 185 7 248 553 35 318 3.73e-35 135
Msa1366480 MtrunA17_Chr2g0295851 27.217 327 206 9 234 554 37 337 2.91e-34 132
Msa1366480 MtrunA17_Chr5g0420831 31.068 309 190 9 251 553 70 361 3.07e-33 130
Msa1366480 MtrunA17_Chr2g0299431 38.767 227 119 6 234 454 40 252 1.56e-32 126
Msa1366480 MtrunA17_Chr8g0351871 29.618 314 187 9 251 553 32 322 1.88e-30 121
Msa1366480 MtrunA17_Chr6g0459781 37.430 179 91 5 251 416 12 182 1.75e-28 112
Msa1366480 MtrunA17_Chr5g0437821 37.879 132 81 1 368 498 4 135 2.27e-26 104
Msa1366480 MtrunA17_Chr4g0025841 29.644 253 158 4 247 499 32 264 3.92e-24 102
Msa1366480 MtrunA17_Chr2g0314691 31.100 209 130 5 346 553 6 201 9.58e-24 99.4
Msa1366480 MtrunA17_Chr7g0265161 33.149 181 88 4 320 495 5 157 1.54e-22 96.7
Msa1366480 MtrunA17_Chr5g0440901 35.294 153 89 3 347 499 1 143 1.77e-22 94.0
Msa1366480 MtrunA17_Chr8g0391281 31.457 302 177 10 41 336 33 310 7.92e-21 93.6
Msa1366480 MtrunA17_Chr5g0440891 33.929 168 99 4 248 414 44 200 1.08e-17 82.0
Msa1366480 MtrunA17_Chr6g0459791 37.903 124 65 3 431 553 4 116 1.03e-15 73.6
Msa1366480 MtrunA17_Chr6g0460141 34.286 140 84 3 283 414 5 144 1.09e-14 71.6
Msa1366480 MtrunA17_Chr4g0073911 28.902 173 110 5 282 446 35 202 1.39e-12 67.8
Msa1366480 MtrunA17_Chr7g0234731 27.155 232 130 8 104 334 121 314 1.42e-12 68.9
Msa1366480 MtrunA17_Chr5g0440881 28.169 142 91 2 412 553 9 139 9.18e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1366480 AT4G02330.1 62.195 574 197 9 8 565 3 572 0.0 713
Msa1366480 AT1G02810.1 62.956 548 180 6 42 566 32 579 0.0 697
Msa1366480 AT2G47550.1 62.547 534 187 7 37 565 34 559 0.0 652
Msa1366480 AT5G04970.1 48.713 544 253 8 41 566 87 622 6.13e-180 521
Msa1366480 AT3G10720.2 48.893 542 255 7 41 566 82 617 7.38e-178 516
Msa1366480 AT2G26440.1 42.182 550 287 10 22 564 22 547 3.10e-130 392
Msa1366480 AT2G45220.1 43.438 541 229 13 40 564 32 511 1.25e-129 389
Msa1366480 AT1G11580.1 40.246 569 300 10 5 564 20 557 2.16e-128 387
Msa1366480 AT1G11580.2 40.246 569 300 10 5 564 41 578 8.12e-128 387
Msa1366480 AT3G49220.1 44.776 469 235 7 98 564 150 596 1.33e-124 379
Msa1366480 AT1G23200.1 41.667 540 277 11 41 564 37 554 1.86e-124 377
Msa1366480 AT3G49220.3 44.776 469 235 7 98 564 195 641 4.00e-124 379
Msa1366480 AT3G10720.1 63.077 260 96 0 306 565 1 260 8.47e-124 365
Msa1366480 AT3G43270.1 44.894 470 233 9 101 566 80 527 1.41e-123 374
Msa1366480 AT3G60730.1 43.008 472 235 9 101 561 68 516 5.25e-121 367
Msa1366480 AT1G53830.1 41.899 537 287 8 40 564 64 587 1.19e-120 368
Msa1366480 AT4G33220.1 45.263 475 230 10 101 564 68 523 1.23e-119 363
Msa1366480 AT1G53840.1 40.399 552 284 14 24 558 58 581 3.26e-119 365
Msa1366480 AT5G51490.1 40.410 537 268 13 42 564 38 536 3.04e-118 360
Msa1366480 AT3G05620.1 41.165 498 234 9 88 566 86 543 3.92e-118 360
Msa1366480 AT5G53370.1 44.280 472 234 10 99 564 137 585 4.28e-118 362
Msa1366480 AT3G14310.1 40.693 548 286 9 40 564 61 592 4.58e-118 362
Msa1366480 AT5G53370.2 44.280 472 234 10 99 564 173 621 1.30e-117 362
Msa1366480 AT4G02300.1 43.011 465 233 8 101 561 93 529 3.61e-117 357
Msa1366480 AT4G02320.1 42.742 496 243 12 77 564 56 518 1.18e-116 356
Msa1366480 AT3G05610.1 40.512 469 251 6 101 565 122 566 3.60e-116 359
Msa1366480 AT4G33220.2 53.012 332 146 3 234 564 80 402 1.14e-115 349
Msa1366480 AT2G43050.1 40.954 503 243 10 69 566 65 518 6.73e-115 351
Msa1366480 AT5G04960.1 43.897 426 215 8 136 560 158 560 8.76e-115 352
Msa1366480 AT5G51500.1 39.377 546 261 13 42 564 42 540 3.17e-112 345
Msa1366480 AT2G26450.1 40.725 469 228 10 101 557 174 604 4.32e-112 347
Msa1366480 AT4G00190.1 40.152 528 250 13 42 564 8 474 4.28e-111 340
Msa1366480 AT3G59010.1 45.266 433 201 10 136 565 130 529 7.06e-111 341
Msa1366480 AT5G27870.1 40.086 464 254 6 98 560 118 558 1.09e-110 347
Msa1366480 AT3G10710.1 39.139 534 280 13 36 564 68 561 6.34e-110 340
Msa1366480 AT4G33230.1 40.043 467 243 10 101 557 160 599 1.49e-107 335
Msa1366480 AT3G14300.1 41.631 466 231 10 101 555 525 960 3.63e-101 328
Msa1366480 AT4G03930.1 35.870 552 294 12 33 564 13 524 2.36e-99 311
Msa1366480 AT5G20860.2 36.609 519 267 13 64 564 64 538 3.53e-99 311
Msa1366480 AT3G06830.1 41.395 430 234 8 136 561 150 565 4.63e-98 309
Msa1366480 AT1G11590.1 35.145 552 298 12 33 564 13 524 2.80e-97 306
Msa1366480 AT5G49180.1 38.972 467 257 8 101 560 121 566 5.41e-97 306
Msa1366480 AT3G47400.1 39.623 477 239 10 101 564 154 594 7.87e-97 307
Msa1366480 AT3G27980.1 48.089 314 157 3 252 564 189 497 2.32e-95 300
Msa1366480 AT4G15980.1 45.161 310 166 2 252 560 392 698 1.09e-83 275
Msa1366480 AT4G15980.2 45.161 310 166 2 252 560 402 708 2.22e-83 275
Msa1366480 AT5G09760.1 45.223 314 159 7 248 552 237 546 3.30e-82 267
Msa1366480 AT5G09760.2 45.223 314 159 7 248 552 263 572 3.54e-82 268
Msa1366480 AT5G64640.1 34.489 548 287 17 41 557 96 602 2.14e-81 266
Msa1366480 AT1G11370.1 50.000 244 119 1 252 495 46 286 9.95e-81 255
Msa1366480 AT5G20860.1 33.399 506 247 12 64 551 64 497 6.36e-79 258
Msa1366480 AT2G47030.1 30.151 597 363 12 8 564 6 588 3.68e-72 242
Msa1366480 AT2G47040.1 30.718 599 364 15 8 564 6 595 1.70e-71 240
Msa1366480 AT3G62170.1 40.909 308 175 4 254 558 279 582 1.73e-66 227
Msa1366480 AT5G19730.1 37.049 305 164 6 260 553 94 381 1.48e-53 187
Msa1366480 AT2G36700.1 33.766 308 184 5 251 553 41 333 3.05e-50 176
Msa1366480 AT2G21610.1 33.227 313 189 6 251 563 50 342 5.22e-50 176
Msa1366480 AT2G21610.2 33.227 313 189 6 251 563 55 347 7.65e-50 176
Msa1366480 AT2G36710.1 34.286 315 186 5 251 558 90 390 1.17e-49 177
Msa1366480 AT1G05310.1 33.333 342 193 7 230 553 64 388 2.66e-49 176
Msa1366480 AT3G29090.1 35.275 309 170 9 251 547 6 296 6.79e-49 172
Msa1366480 AT5G07430.1 32.515 326 199 6 232 553 47 355 1.28e-46 167
Msa1366480 AT3G17060.1 30.312 320 194 7 244 554 38 337 3.74e-44 160
Msa1366480 AT5G18990.1 34.483 290 157 7 252 527 29 299 7.56e-41 151
Msa1366480 AT5G47500.1 30.503 318 186 8 251 553 60 357 1.61e-40 151
Msa1366480 AT3G24130.1 33.220 295 164 7 247 527 29 304 1.18e-39 148
Msa1366480 AT2G19150.1 31.529 314 195 7 252 560 41 339 1.24e-39 148
Msa1366480 AT5G55590.1 30.818 318 191 7 248 553 78 378 1.09e-37 143
Msa1366480 AT5G07420.1 29.773 309 196 6 249 553 64 355 4.49e-37 141
Msa1366480 AT1G44980.1 45.509 167 88 1 252 418 78 241 1.88e-36 136
Msa1366480 AT1G69940.1 28.354 328 211 9 231 553 47 355 5.65e-36 138
Msa1366480 AT5G61680.1 30.421 309 191 8 251 553 68 358 6.12e-36 138
Msa1366480 AT5G07410.1 28.354 328 211 9 231 553 47 355 2.33e-35 136
Msa1366480 AT2G47280.1 30.063 316 188 8 252 555 35 329 5.92e-35 135
Msa1366480 AT5G55590.2 31.939 263 152 6 248 498 78 325 2.31e-34 133
Msa1366480 AT5G26810.1 31.928 332 187 11 240 553 23 333 1.12e-33 131
Msa1366480 AT2G19150.2 32.941 255 153 6 252 501 41 282 2.07e-33 129
Msa1366480 AT3G49220.2 31.381 239 141 6 98 335 195 411 2.82e-20 94.0

Find 120 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTTACATGCAAACATTTA+TGG 0.129976 tig0018459:+64555 Msa1366480:CDS
TGGTGCTGTTTCAACATATT+TGG 0.148909 tig0018459:+64504 Msa1366480:CDS
TCGCTAGAAACAGATTTATT+TGG 0.177140 tig0018459:-63219 None:intergenic
TACAGAGAATGTGACATTTA+TGG 0.181984 tig0018459:+64295 Msa1366480:CDS
GTGGATAGAGGTTACAATTT+TGG 0.190584 tig0018459:-64357 None:intergenic
AGACATTAATTAATTAATTA+AGG 0.202828 tig0018459:-64806 None:intergenic
AAAGCTAACGTTTATGATTA+TGG 0.233483 tig0018459:+62577 Msa1366480:CDS
TATGCTGAGTTTAATAATAC+TGG 0.243616 tig0018459:+64643 Msa1366480:CDS
GTTGGAGATATTTGTTGATT+AGG 0.292074 tig0018459:-62648 None:intergenic
AAATCCTTTGTTAGAGTGTT+TGG 0.294966 tig0018459:-63013 None:intergenic
GGAACAGTAGATTTCATATT+CGG 0.296112 tig0018459:+64316 Msa1366480:CDS
ACATAGCTTGGAAGCCATAT+TGG 0.314129 tig0018459:-62398 None:intergenic
CCTGGTGAGATAGGTGTAAT+TGG 0.334751 tig0018459:-62496 None:intergenic
TGCTTTCTTTACTAAAGGTT+GGG 0.335009 tig0018459:+62954 Msa1366480:CDS
GACAAACCAACAAACATGTT+TGG 0.337556 tig0018459:+62840 Msa1366480:CDS
GAGATAGGTGTAATTGGATT+TGG 0.345211 tig0018459:-62490 None:intergenic
TTGCTTTCTTTACTAAAGGT+TGG 0.347374 tig0018459:+62953 Msa1366480:CDS
ATGTATAATTACCGAATGTT+GGG 0.354549 tig0018459:-63398 None:intergenic
TCTCTTGCTTTCTTTACTAA+AGG 0.356675 tig0018459:+62949 Msa1366480:CDS
GGTGCTGTTTCAACATATTT+GGG 0.357572 tig0018459:+64505 Msa1366480:CDS
CATGCTGCTCCTGGTGAGAT+AGG 0.380293 tig0018459:-62505 None:intergenic
AAGCAGTGGCACTTAGAAAC+GGG 0.398121 tig0018459:+64194 Msa1366480:CDS
ATGTTGCTGGGTGGCGTGCT+TGG 0.401190 tig0018459:+64590 Msa1366480:CDS
AAGAGAGACACTATAGAGTT+TGG 0.403782 tig0018459:-62932 None:intergenic
GACCATCCAAACATGTTTGT+TGG 0.404138 tig0018459:-62846 None:intergenic
AACTACTTTGTAAAATAAGT+TGG 0.405498 tig0018459:+64917 Msa1366480:three_prime_UTR
ATTTCTTGCTTGGTGATGAT+TGG 0.405620 tig0018459:+64752 Msa1366480:CDS
GATGTACCTGTGTCTTGGTT+AGG 0.407899 tig0018459:-64433 None:intergenic
GAATCGCGCTGAGCAATGTT+TGG 0.411363 tig0018459:-62816 None:intergenic
GATGATTGGTTGCCTCAAAC+AGG 0.413849 tig0018459:+64766 Msa1366480:CDS
TGGGAGAATGGATTTGCAAT+AGG 0.413977 tig0018459:-62548 None:intergenic
AATGTATAATTACCGAATGT+TGG 0.421859 tig0018459:-63399 None:intergenic
GTGGATTTGCATGCTGCTCC+TGG 0.426506 tig0018459:-62514 None:intergenic
GATTTGCAATAGGTTGGGTC+AGG 0.427569 tig0018459:-62538 None:intergenic
TTGCTTAATTCAACATTTGT+CGG 0.441396 tig0018459:-64971 None:intergenic
ACGGTGGCAAATTTCTTGCT+TGG 0.445744 tig0018459:+64742 Msa1366480:CDS
GCATTGAATTGTCCACTCAT+TGG 0.449744 tig0018459:-64385 None:intergenic
TGTTGCTGGGTGGCGTGCTT+GGG 0.450946 tig0018459:+64591 Msa1366480:CDS
AATAAATCTGTTTCTAGCGA+TGG 0.450968 tig0018459:+63222 Msa1366480:CDS
TTGCTTTAATTGTACAGTTG+TGG 0.453845 tig0018459:-64459 None:intergenic
CGTTTCTAAGTGCCACTGCT+TGG 0.461010 tig0018459:-64192 None:intergenic
ATGAATGTTGCAGCTGTGGT+TGG 0.462240 tig0018459:+64112 Msa1366480:intron
TAGTGCTCGCACAGCGGTGG+TGG 0.463374 tig0018459:-62686 None:intergenic
TGAGAGGGTATTACATAGCT+TGG 0.463691 tig0018459:-62410 None:intergenic
GTGTAAGACACCCCTGTTTG+AGG 0.465284 tig0018459:-64778 None:intergenic
AGAATGGATTTGCAATAGGT+TGG 0.473235 tig0018459:-62544 None:intergenic
GAATGGATTTGCAATAGGTT+GGG 0.475452 tig0018459:-62543 None:intergenic
TACAAAGTAGTTATAGTGGA+AGG 0.476675 tig0018459:-64906 None:intergenic
GATAATGTGATTAATGTTGC+TGG 0.479559 tig0018459:+64577 Msa1366480:CDS
GCTGGGTGGCGTGCTTGGGA+TGG 0.482960 tig0018459:+64595 Msa1366480:CDS
CAAGCAGTGGCACTTAGAAA+CGG 0.489043 tig0018459:+64193 Msa1366480:CDS
CAGTTTGAAAAGAGTTTGAA+AGG 0.492675 tig0018459:-62747 None:intergenic
TGTGGTTGGGCAAGGATTCG+TGG 0.498726 tig0018459:+64126 Msa1366480:CDS
ATCACAGTGTTGTTGATGGC+TGG 0.499298 tig0018459:+63361 Msa1366480:CDS
GTTGATGGCTGGACCACATT+CGG 0.499631 tig0018459:+63372 Msa1366480:CDS
AGTTGAGGTCGCTGAGCGAT+TGG 0.500496 tig0018459:-62717 None:intergenic
GGCAATCACAGTGTTGTTGA+TGG 0.508915 tig0018459:+63357 Msa1366480:CDS
GGATTGATGTACCTGTGTCT+TGG 0.509046 tig0018459:-64438 None:intergenic
TTTGCAATAGGTTGGGTCAG+GGG 0.511394 tig0018459:-62536 None:intergenic
TTTCATATTCGGAAATGCTA+AGG 0.512250 tig0018459:+64327 Msa1366480:CDS
TGAATGTTGCAGCTGTGGTT+GGG 0.516948 tig0018459:+64113 Msa1366480:intron
AAGTTTCTTCCAAGTTTACA+AGG 0.517405 tig0018459:+62784 Msa1366480:CDS
GAGTTTAATAATACTGGGCC+AGG 0.520794 tig0018459:+64649 Msa1366480:CDS
ATTGTGACGGTTAGTCAAGA+TGG 0.521081 tig0018459:+63153 Msa1366480:CDS
CACAGCGGTGGTGGAAAGAG+TGG 0.524616 tig0018459:-62677 None:intergenic
AGTTTGGTGTCATTAGAAAG+TGG 0.527999 tig0018459:-62916 None:intergenic
ATAAATCTGTTTCTAGCGAT+GGG 0.535312 tig0018459:+63223 Msa1366480:CDS
AACCAACAAACATGTTTGGA+TGG 0.537810 tig0018459:+62844 Msa1366480:CDS
ACATTCGGCTCCCCAACATT+CGG 0.540697 tig0018459:+63387 Msa1366480:CDS
CTACCATCAGTGCTAGAGCC+TGG 0.540713 tig0018459:-64667 None:intergenic
TCATTGGAAGACGTGGATAG+AGG 0.541407 tig0018459:-64369 None:intergenic
CGTAAAATTAGCTGCATCCG+TGG 0.542453 tig0018459:-64722 None:intergenic
GTTCTAGAATACTCTTTCCA+TGG 0.544216 tig0018459:-64532 None:intergenic
AATTGGATTTGGACTAGTAC+CGG 0.546025 tig0018459:-62479 None:intergenic
TTAAAGACACATCTTCAGCT+TGG 0.547064 tig0018459:+62869 Msa1366480:CDS
GCAATAGGTTGGGTCAGGGG+TGG 0.547288 tig0018459:-62533 None:intergenic
AATTCCAAACACTCTAACAA+AGG 0.556033 tig0018459:+63009 Msa1366480:CDS
ATTTGCAATAGGTTGGGTCA+GGG 0.557363 tig0018459:-62537 None:intergenic
GTTGCAGCTGTGGTTGGGCA+AGG 0.558786 tig0018459:+64118 Msa1366480:intron
TGGAAAGAGTGGAGCGACGT+TGG 0.560848 tig0018459:-62666 None:intergenic
ACGGTTAGTCAAGATGGAAG+TGG 0.570515 tig0018459:+63159 Msa1366480:CDS
CCAATTACACCTATCTCACC+AGG 0.573664 tig0018459:+62496 Msa1366480:CDS
GTGGTTGGGCAAGGATTCGT+GGG 0.574159 tig0018459:+64127 Msa1366480:CDS
CAACATATTTGGGAAGACCA+TGG 0.574540 tig0018459:+64515 Msa1366480:CDS
GATGATTTAGCTTCTAGCAA+TGG 0.582638 tig0018459:+64484 Msa1366480:CDS
ATGCTGAGTTTAATAATACT+GGG 0.583978 tig0018459:+64644 Msa1366480:CDS
AAAGAATGAGAAATATTGAG+AGG 0.586083 tig0018459:-62426 None:intergenic
ATTAACAAGACTATTATCAC+TGG 0.586139 tig0018459:+63336 Msa1366480:CDS
GGGCCAGGCTCTAGCACTGA+TGG 0.587768 tig0018459:+64664 Msa1366480:CDS
TTGTAGTGCTCGCACAGCGG+TGG 0.588898 tig0018459:-62689 None:intergenic
CTATCGTGTGATTAATGCCA+CGG 0.589574 tig0018459:+64705 Msa1366480:CDS
TTTACAGTTGCAGTTTCGAA+GGG 0.589810 tig0018459:+64233 Msa1366480:CDS
TCAAGTTGGTGAGGATGTTG+TGG 0.590169 tig0018459:+63122 Msa1366480:CDS
TGCTGTGAAGCACCAAGCAG+TGG 0.592757 tig0018459:+64180 Msa1366480:CDS
GATGGTAGAGTGACGTGGCA+AGG 0.596651 tig0018459:+64682 Msa1366480:CDS
GAATGTTGGGGAGCCGAATG+TGG 0.602659 tig0018459:-63385 None:intergenic
ATGACCATTCGTAACACAGC+AGG 0.603116 tig0018459:+64157 Msa1366480:CDS
TCAGAGTGATCAAGTTGGTG+AGG 0.605937 tig0018459:+63113 Msa1366480:CDS
AAGAATGAGAAATATTGAGA+GGG 0.609418 tig0018459:-62425 None:intergenic
AATGTGTGTATAACGTGTCT+TGG 0.609557 tig0018459:-64258 None:intergenic
CTTCTTCAGAGTGATCAAGT+TGG 0.613368 tig0018459:+63108 Msa1366480:CDS
ATGGAAAGAGTATTCTAGAA+CGG 0.614937 tig0018459:+64534 Msa1366480:CDS
ATGATTGGTTGCCTCAAACA+GGG 0.616738 tig0018459:+64767 Msa1366480:CDS
TGATTGGTTGCCTCAAACAG+GGG 0.618420 tig0018459:+64768 Msa1366480:CDS
TATCCACGTCTTCCAATGAG+TGG 0.622034 tig0018459:+64373 Msa1366480:CDS
TTCAATGCAATCACTGCACA+AGG 0.625555 tig0018459:+64400 Msa1366480:CDS
TTGAAAGGAAATCAGAGTTG+AGG 0.625641 tig0018459:-62732 None:intergenic
TGTATAATTACCGAATGTTG+GGG 0.625668 tig0018459:-63397 None:intergenic
ATTGCTAGAAGCTAAATCAT+CGG 0.626819 tig0018459:-64482 None:intergenic
AGCACCTGCTGTGTTACGAA+TGG 0.627389 tig0018459:-64161 None:intergenic
ATAATGTGATTAATGTTGCT+GGG 0.635060 tig0018459:+64578 Msa1366480:CDS
TGTCCACTCATTGGAAGACG+TGG 0.636776 tig0018459:-64376 None:intergenic
CGATTTATGAGTCAGTTAGT+CGG 0.649371 tig0018459:+63079 Msa1366480:CDS
TTTATGAGTCAGTTAGTCGG+AGG 0.663720 tig0018459:+63082 Msa1366480:CDS
ACAGATCCTAACCAAGACAC+AGG 0.667761 tig0018459:+64427 Msa1366480:CDS
ATCTTGTAGTGCTCGCACAG+CGG 0.715861 tig0018459:-62692 None:intergenic
TGTGGTGAGAGATATTGTGA+CGG 0.718292 tig0018459:+63140 Msa1366480:CDS
AGCAGTGGCACTTAGAAACG+GGG 0.723770 tig0018459:+64195 Msa1366480:CDS
GCACTGATGGTAGAGTGACG+TGG 0.734964 tig0018459:+64677 Msa1366480:CDS
ATGTGATTAATGTTGCTGGG+TGG 0.776487 tig0018459:+64581 Msa1366480:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAAACCAATA+TGG + tig0018459:62384-62403 Msa1366480:exon 10.0%
!!! TTTAAGTTTTAGTTTTTTTT+AGG - tig0018459:63501-63520 None:intergenic 10.0%
!! AGACATTAATTAATTAATTA+AGG - tig0018459:64809-64828 None:intergenic 10.0%
!! AGAAATAATTTAACTATTGT+GGG + tig0018459:63559-63578 Msa1366480:intron 15.0%
!! AATTATTAAATTGAAAACAG+AGG - tig0018459:63662-63681 None:intergenic 15.0%
!!! ATCAAGTAAAGATTTTATAT+AGG + tig0018459:64026-64045 Msa1366480:intron 15.0%
!! TCTAACAAAGGATTTAAAAA+CGG + tig0018459:63021-63040 Msa1366480:CDS 20.0%
!! TTGATGAAAAAATATAGAAC+TGG - tig0018459:63426-63445 None:intergenic 20.0%
!!! AAACTTAAAGACATTTTTAC+TGG + tig0018459:63512-63531 Msa1366480:intron 20.0%
!! GAGAAATAATTTAACTATTG+TGG + tig0018459:63558-63577 Msa1366480:intron 20.0%
!! GAAATAATTTAACTATTGTG+GGG + tig0018459:63560-63579 Msa1366480:intron 20.0%
!!! ATCGTTTTTGACATATTTTA+GGG - tig0018459:63627-63646 None:intergenic 20.0%
!!! TATCGTTTTTGACATATTTT+AGG - tig0018459:63628-63647 None:intergenic 20.0%
!! ACACAAAATATATACATTAG+CGG + tig0018459:63901-63920 Msa1366480:intron 20.0%
!! GGTATGTTAAAAAAAATGTT+TGG - tig0018459:63932-63951 None:intergenic 20.0%
!!! AGTTTTATAAATAAAGACTG+AGG + tig0018459:63975-63994 Msa1366480:intron 20.0%
!!! ATTTTACAAAGTAGTTATAG+TGG - tig0018459:64913-64932 None:intergenic 20.0%
!! AACTACTTTGTAAAATAAGT+TGG + tig0018459:64917-64936 Msa1366480:three_prime_UTR 20.0%
! AAGAATGAGAAATATTGAGA+GGG - tig0018459:62428-62447 None:intergenic 25.0%
! AAAGAATGAGAAATATTGAG+AGG - tig0018459:62429-62448 None:intergenic 25.0%
! AAAGCTAACGTTTATGATTA+TGG + tig0018459:62577-62596 Msa1366480:CDS 25.0%
!! ATATTTTCTCCTTGTAAACT+TGG - tig0018459:62796-62815 None:intergenic 25.0%
! CTAACAAAGGATTTAAAAAC+GGG + tig0018459:63022-63041 Msa1366480:CDS 25.0%
!!! AAGACTTTTTTGATGATGAT+TGG + tig0018459:63306-63325 Msa1366480:CDS 25.0%
! ATTAACAAGACTATTATCAC+TGG + tig0018459:63336-63355 Msa1366480:CDS 25.0%
! ATGTATAATTACCGAATGTT+GGG - tig0018459:63401-63420 None:intergenic 25.0%
! AATGTATAATTACCGAATGT+TGG - tig0018459:63402-63421 None:intergenic 25.0%
! TTTATACGTTTATGCACAAA+AGG + tig0018459:63842-63861 Msa1366480:intron 25.0%
! ATAATGTGATTAATGTTGCT+GGG + tig0018459:64578-64597 Msa1366480:CDS 25.0%
! TATGCTGAGTTTAATAATAC+TGG + tig0018459:64643-64662 Msa1366480:CDS 25.0%
! ATGCTGAGTTTAATAATACT+GGG + tig0018459:64644-64663 Msa1366480:CDS 25.0%
!! TCACTTTGTTCTTTTTCTTT+TGG + tig0018459:64876-64895 Msa1366480:three_prime_UTR 25.0%
!! CACTTTGTTCTTTTTCTTTT+GGG + tig0018459:64877-64896 Msa1366480:three_prime_UTR 25.0%
! TTGCTTAATTCAACATTTGT+CGG - tig0018459:64974-64993 None:intergenic 25.0%
! ATAGCATTTTCACAAAACAC+CGG + tig0018459:62460-62479 Msa1366480:CDS 30.0%
!!! TCATAAACGTTAGCTTTTTG+AGG - tig0018459:62574-62593 None:intergenic 30.0%
! GTTGGAGATATTTGTTGATT+AGG - tig0018459:62651-62670 None:intergenic 30.0%
CAGTTTGAAAAGAGTTTGAA+AGG - tig0018459:62750-62769 None:intergenic 30.0%
AAGTTTCTTCCAAGTTTACA+AGG + tig0018459:62784-62803 Msa1366480:CDS 30.0%
TCTCTTGCTTTCTTTACTAA+AGG + tig0018459:62949-62968 Msa1366480:CDS 30.0%
TTGCTTTCTTTACTAAAGGT+TGG + tig0018459:62953-62972 Msa1366480:CDS 30.0%
TGCTTTCTTTACTAAAGGTT+GGG + tig0018459:62954-62973 Msa1366480:CDS 30.0%
!! ATTTTGAAAGGCGGTTTTAT+TGG - tig0018459:62992-63011 None:intergenic 30.0%
!! AGAGTGTTTGGAATTTTGAA+AGG - tig0018459:63004-63023 None:intergenic 30.0%
AATTCCAAACACTCTAACAA+AGG + tig0018459:63009-63028 Msa1366480:CDS 30.0%
! AAATCCTTTGTTAGAGTGTT+TGG - tig0018459:63016-63035 None:intergenic 30.0%
TCGCTAGAAACAGATTTATT+TGG - tig0018459:63222-63241 None:intergenic 30.0%
!! AATAAATCTGTTTCTAGCGA+TGG + tig0018459:63222-63241 Msa1366480:CDS 30.0%
!! ATAAATCTGTTTCTAGCGAT+GGG + tig0018459:63223-63242 Msa1366480:CDS 30.0%
! TTTTTGATCTATGTTACTGC+TGG + tig0018459:63249-63268 Msa1366480:CDS 30.0%
!!! TTTTTGATGATGATTGGAGA+TGG + tig0018459:63312-63331 Msa1366480:CDS 30.0%
TGTATAATTACCGAATGTTG+GGG - tig0018459:63400-63419 None:intergenic 30.0%
TACAGAGAATGTGACATTTA+TGG + tig0018459:64295-64314 Msa1366480:CDS 30.0%
GGAACAGTAGATTTCATATT+CGG + tig0018459:64316-64335 Msa1366480:CDS 30.0%
TTTCATATTCGGAAATGCTA+AGG + tig0018459:64327-64346 Msa1366480:CDS 30.0%
TTGCTTTAATTGTACAGTTG+TGG - tig0018459:64462-64481 None:intergenic 30.0%
ATTGCTAGAAGCTAAATCAT+CGG - tig0018459:64485-64504 None:intergenic 30.0%
!! ATGGAAAGAGTATTCTAGAA+CGG + tig0018459:64534-64553 Msa1366480:CDS 30.0%
GGTTTACATGCAAACATTTA+TGG + tig0018459:64555-64574 Msa1366480:CDS 30.0%
GATAATGTGATTAATGTTGC+TGG + tig0018459:64577-64596 Msa1366480:CDS 30.0%
! TACAAAGTAGTTATAGTGGA+AGG - tig0018459:64909-64928 None:intergenic 30.0%
!! AATTGGATTTGGACTAGTAC+CGG - tig0018459:62482-62501 None:intergenic 35.0%
GAGATAGGTGTAATTGGATT+TGG - tig0018459:62493-62512 None:intergenic 35.0%
! GAATGGATTTGCAATAGGTT+GGG - tig0018459:62546-62565 None:intergenic 35.0%
! AGAATGGATTTGCAATAGGT+TGG - tig0018459:62547-62566 None:intergenic 35.0%
!!! AAACGTTAGCTTTTTGAGGT+GGG - tig0018459:62570-62589 None:intergenic 35.0%
!!! TAAACGTTAGCTTTTTGAGG+TGG - tig0018459:62571-62590 None:intergenic 35.0%
TTGAAAGGAAATCAGAGTTG+AGG - tig0018459:62735-62754 None:intergenic 35.0%
GACAAACCAACAAACATGTT+TGG + tig0018459:62840-62859 Msa1366480:CDS 35.0%
AACCAACAAACATGTTTGGA+TGG + tig0018459:62844-62863 Msa1366480:CDS 35.0%
TTAAAGACACATCTTCAGCT+TGG + tig0018459:62869-62888 Msa1366480:CDS 35.0%
!! AGTTTGGTGTCATTAGAAAG+TGG - tig0018459:62919-62938 None:intergenic 35.0%
AAGAGAGACACTATAGAGTT+TGG - tig0018459:62935-62954 None:intergenic 35.0%
!!! AAGGCGGTTTTATTGGTTTT+GGG - tig0018459:62985-63004 None:intergenic 35.0%
!!! AAAGGCGGTTTTATTGGTTT+TGG - tig0018459:62986-63005 None:intergenic 35.0%
!! GTGTTTGGAATTTTGAAAGG+CGG - tig0018459:63001-63020 None:intergenic 35.0%
CGATTTATGAGTCAGTTAGT+CGG + tig0018459:63079-63098 Msa1366480:CDS 35.0%
! TTTTATGAATGTTGCAGCTG+TGG + tig0018459:64108-64127 Msa1366480:intron 35.0%
! TTTTACAGTTGCAGTTTCGA+AGG + tig0018459:64232-64251 Msa1366480:CDS 35.0%
TTTACAGTTGCAGTTTCGAA+GGG + tig0018459:64233-64252 Msa1366480:CDS 35.0%
AATGTGTGTATAACGTGTCT+TGG - tig0018459:64261-64280 None:intergenic 35.0%
! GTGGATAGAGGTTACAATTT+TGG - tig0018459:64360-64379 None:intergenic 35.0%
! GATGATTTAGCTTCTAGCAA+TGG + tig0018459:64484-64503 Msa1366480:CDS 35.0%
!! TGGTGCTGTTTCAACATATT+TGG + tig0018459:64504-64523 Msa1366480:CDS 35.0%
!! GGTGCTGTTTCAACATATTT+GGG + tig0018459:64505-64524 Msa1366480:CDS 35.0%
!! GTTCTAGAATACTCTTTCCA+TGG - tig0018459:64535-64554 None:intergenic 35.0%
!! ATTTCTTGCTTGGTGATGAT+TGG + tig0018459:64752-64771 Msa1366480:CDS 35.0%
ACATAGCTTGGAAGCCATAT+TGG - tig0018459:62401-62420 None:intergenic 40.0%
!! TGAGAGGGTATTACATAGCT+TGG - tig0018459:62413-62432 None:intergenic 40.0%
! ATTTGCAATAGGTTGGGTCA+GGG - tig0018459:62540-62559 None:intergenic 40.0%
! TGGGAGAATGGATTTGCAAT+AGG - tig0018459:62551-62570 None:intergenic 40.0%
GACCATCCAAACATGTTTGT+TGG - tig0018459:62849-62868 None:intergenic 40.0%
! TCAGCTTGGAGTTTTCGAAA+TGG + tig0018459:62883-62902 Msa1366480:CDS 40.0%
! GTTTTGCTCGTCATCTTAAG+CGG - tig0018459:63054-63073 None:intergenic 40.0%
TTTATGAGTCAGTTAGTCGG+AGG + tig0018459:63082-63101 Msa1366480:CDS 40.0%
CTTCTTCAGAGTGATCAAGT+TGG + tig0018459:63108-63127 Msa1366480:CDS 40.0%
TGTGGTGAGAGATATTGTGA+CGG + tig0018459:63140-63159 Msa1366480:CDS 40.0%
! ATTGTGACGGTTAGTCAAGA+TGG + tig0018459:63153-63172 Msa1366480:CDS 40.0%
GCATTGAATTGTCCACTCAT+TGG - tig0018459:64388-64407 None:intergenic 40.0%
TTCAATGCAATCACTGCACA+AGG + tig0018459:64400-64419 Msa1366480:CDS 40.0%
CAACATATTTGGGAAGACCA+TGG + tig0018459:64515-64534 Msa1366480:CDS 40.0%
ATGTGATTAATGTTGCTGGG+TGG + tig0018459:64581-64600 Msa1366480:CDS 40.0%
GAGTTTAATAATACTGGGCC+AGG + tig0018459:64649-64668 Msa1366480:CDS 40.0%
CTATCGTGTGATTAATGCCA+CGG + tig0018459:64705-64724 Msa1366480:CDS 40.0%
! CACGGATGCAGCTAATTTTA+CGG + tig0018459:64723-64742 Msa1366480:CDS 40.0%
! ATGATTGGTTGCCTCAAACA+GGG + tig0018459:64767-64786 Msa1366480:CDS 40.0%
CCTGGTGAGATAGGTGTAAT+TGG - tig0018459:62499-62518 None:intergenic 45.0%
CCAATTACACCTATCTCACC+AGG + tig0018459:62496-62515 Msa1366480:CDS 45.0%
! TTTGCAATAGGTTGGGTCAG+GGG - tig0018459:62539-62558 None:intergenic 45.0%
! GATTTGCAATAGGTTGGGTC+AGG - tig0018459:62541-62560 None:intergenic 45.0%
!!! AGCTTTTTGAGGTGGGAGAA+TGG - tig0018459:62563-62582 None:intergenic 45.0%
!! TCAGAGTGATCAAGTTGGTG+AGG + tig0018459:63113-63132 Msa1366480:CDS 45.0%
!! TCAAGTTGGTGAGGATGTTG+TGG + tig0018459:63122-63141 Msa1366480:CDS 45.0%
! ACGGTTAGTCAAGATGGAAG+TGG + tig0018459:63159-63178 Msa1366480:CDS 45.0%
! GGCAATCACAGTGTTGTTGA+TGG + tig0018459:63357-63376 Msa1366480:CDS 45.0%
!! ATCACAGTGTTGTTGATGGC+TGG + tig0018459:63361-63380 Msa1366480:CDS 45.0%
ATTGTGGGGCTCACACATAA+TGG + tig0018459:63574-63593 Msa1366480:intron 45.0%
ATGAATGTTGCAGCTGTGGT+TGG + tig0018459:64112-64131 Msa1366480:intron 45.0%
TGAATGTTGCAGCTGTGGTT+GGG + tig0018459:64113-64132 Msa1366480:intron 45.0%
ATGACCATTCGTAACACAGC+AGG + tig0018459:64157-64176 Msa1366480:CDS 45.0%
CAAGCAGTGGCACTTAGAAA+CGG + tig0018459:64193-64212 Msa1366480:CDS 45.0%
AAGCAGTGGCACTTAGAAAC+GGG + tig0018459:64194-64213 Msa1366480:CDS 45.0%
TCATTGGAAGACGTGGATAG+AGG - tig0018459:64372-64391 None:intergenic 45.0%
TATCCACGTCTTCCAATGAG+TGG + tig0018459:64373-64392 Msa1366480:CDS 45.0%
ACAGATCCTAACCAAGACAC+AGG + tig0018459:64427-64446 Msa1366480:CDS 45.0%
GATGTACCTGTGTCTTGGTT+AGG - tig0018459:64436-64455 None:intergenic 45.0%
GGATTGATGTACCTGTGTCT+TGG - tig0018459:64441-64460 None:intergenic 45.0%
CGTAAAATTAGCTGCATCCG+TGG - tig0018459:64725-64744 None:intergenic 45.0%
! GGATGCAGCTAATTTTACGG+TGG + tig0018459:64726-64745 Msa1366480:CDS 45.0%
ACGGTGGCAAATTTCTTGCT+TGG + tig0018459:64742-64761 Msa1366480:CDS 45.0%
! GATGATTGGTTGCCTCAAAC+AGG + tig0018459:64766-64785 Msa1366480:CDS 45.0%
! TGATTGGTTGCCTCAAACAG+GGG + tig0018459:64768-64787 Msa1366480:CDS 45.0%
!!! TTTTTTTTATAATATTTTTC+AGG - tig0018459:63953-63972 None:intergenic 5.0%
ATCTTGTAGTGCTCGCACAG+CGG - tig0018459:62695-62714 None:intergenic 50.0%
GAATCGCGCTGAGCAATGTT+TGG - tig0018459:62819-62838 None:intergenic 50.0%
!! GTTGATGGCTGGACCACATT+CGG + tig0018459:63372-63391 Msa1366480:CDS 50.0%
ACATTCGGCTCCCCAACATT+CGG + tig0018459:63387-63406 Msa1366480:CDS 50.0%
! TGGGGCTCACACATAATGGA+GGG + tig0018459:63578-63597 Msa1366480:intron 50.0%
AGCACCTGCTGTGTTACGAA+TGG - tig0018459:64164-64183 None:intergenic 50.0%
! CGTTTCTAAGTGCCACTGCT+TGG - tig0018459:64195-64214 None:intergenic 50.0%
AGCAGTGGCACTTAGAAACG+GGG + tig0018459:64195-64214 Msa1366480:CDS 50.0%
TGTCCACTCATTGGAAGACG+TGG - tig0018459:64379-64398 None:intergenic 50.0%
GTGTAAGACACCCCTGTTTG+AGG - tig0018459:64781-64800 None:intergenic 50.0%
CATGCTGCTCCTGGTGAGAT+AGG - tig0018459:62508-62527 None:intergenic 55.0%
GTGGATTTGCATGCTGCTCC+TGG - tig0018459:62517-62536 None:intergenic 55.0%
TGGAAAGAGTGGAGCGACGT+TGG - tig0018459:62669-62688 None:intergenic 55.0%
AGTTGAGGTCGCTGAGCGAT+TGG - tig0018459:62720-62739 None:intergenic 55.0%
GAATGTTGGGGAGCCGAATG+TGG - tig0018459:63388-63407 None:intergenic 55.0%
GTGGGGCTCACACATAATGG+AGG + tig0018459:63577-63596 Msa1366480:intron 55.0%
TGTGGTTGGGCAAGGATTCG+TGG + tig0018459:64126-64145 Msa1366480:CDS 55.0%
GTGGTTGGGCAAGGATTCGT+GGG + tig0018459:64127-64146 Msa1366480:CDS 55.0%
! TGCTGTGAAGCACCAAGCAG+TGG + tig0018459:64180-64199 Msa1366480:CDS 55.0%
CTACCATCAGTGCTAGAGCC+TGG - tig0018459:64670-64689 None:intergenic 55.0%
! GCACTGATGGTAGAGTGACG+TGG + tig0018459:64677-64696 Msa1366480:CDS 55.0%
GATGGTAGAGTGACGTGGCA+AGG + tig0018459:64682-64701 Msa1366480:CDS 55.0%
! GCAATAGGTTGGGTCAGGGG+TGG - tig0018459:62536-62555 None:intergenic 60.0%
!! CACAGCGGTGGTGGAAAGAG+TGG - tig0018459:62680-62699 None:intergenic 60.0%
TTGTAGTGCTCGCACAGCGG+TGG - tig0018459:62692-62711 None:intergenic 60.0%
GTTGCAGCTGTGGTTGGGCA+AGG + tig0018459:64118-64137 Msa1366480:intron 60.0%
ATGTTGCTGGGTGGCGTGCT+TGG + tig0018459:64590-64609 Msa1366480:CDS 60.0%
TGTTGCTGGGTGGCGTGCTT+GGG + tig0018459:64591-64610 Msa1366480:CDS 60.0%
TAGTGCTCGCACAGCGGTGG+TGG - tig0018459:62689-62708 None:intergenic 65.0%
! GGGCCAGGCTCTAGCACTGA+TGG + tig0018459:64664-64683 Msa1366480:CDS 65.0%
GCTGGGTGGCGTGCTTGGGA+TGG + tig0018459:64595-64614 Msa1366480:CDS 70.0%
Chromosome Type Strat End Strand Name
tig0018459 gene 62379 65038 62379 ID=Msa1366480;Name=Msa1366480
tig0018459 mRNA 62379 65038 62379 ID=Msa1366480-mRNA-1;Parent=Msa1366480;Name=Msa1366480-mRNA-1;_AED=0.23;_eAED=0.23;_QI=24|1|1|1|1|1|2|219|566
tig0018459 exon 62379 63408 62379 ID=Msa1366480-mRNA-1:exon:15164;Parent=Msa1366480-mRNA-1
tig0018459 exon 64125 65038 64125 ID=Msa1366480-mRNA-1:exon:15165;Parent=Msa1366480-mRNA-1
tig0018459 five_prime_UTR 62379 62402 62379 ID=Msa1366480-mRNA-1:five_prime_utr;Parent=Msa1366480-mRNA-1
tig0018459 CDS 62403 63408 62403 ID=Msa1366480-mRNA-1:cds;Parent=Msa1366480-mRNA-1
tig0018459 CDS 64125 64819 64125 ID=Msa1366480-mRNA-1:cds;Parent=Msa1366480-mRNA-1
tig0018459 three_prime_UTR 64820 65038 64820 ID=Msa1366480-mRNA-1:three_prime_utr;Parent=Msa1366480-mRNA-1
Gene Sequence

>Msa1366480

ATGGCTTCCAAGCTATGTAATACCCTCTCAATATTTCTCATTCTTTTATCTGTCTCTATAGCATTTTCACAAAACACCGGTACTAGTCCAAATCCAATTACACCTATCTCACCAGGAGCAGCATGCAAATCCACCCCTGACCCAACCTATTGCAAATCCATTCTCCCACCTCAAAAAGCTAACGTTTATGATTATGGTCGATTTTCAGTCAAAAAATCACTATCACAATCTCGACAATTCTCAAACCTAATCAACAAATATCTCCAACGTCGCTCCACTCTTTCCACCACCGCTGTGCGAGCACTACAAGATTGCCAATCGCTCAGCGACCTCAACTCTGATTTCCTTTCAAACTCTTTTCAAACTGTTAACAAAACAACAAAGTTTCTTCCAAGTTTACAAGGAGAAAATATCCAAACATTGCTCAGCGCGATTCTGACAAACCAACAAACATGTTTGGATGGTCTTAAAGACACATCTTCAGCTTGGAGTTTTCGAAATGGACTCACAATTCCACTTTCTAATGACACCAAACTCTATAGTGTCTCTCTTGCTTTCTTTACTAAAGGTTGGGTTAATCCCAAAACCAATAAAACCGCCTTTCAAAATTCCAAACACTCTAACAAAGGATTTAAAAACGGGCGTCTACCGCTTAAGATGACGAGCAAAACACGCGCGATTTATGAGTCAGTTAGTCGGAGGAAACTTCTTCAGAGTGATCAAGTTGGTGAGGATGTTGTGGTGAGAGATATTGTGACGGTTAGTCAAGATGGAAGTGGAAACTTTACTACGATTAATGATGCTATTGCTGCAGCTCCAAATAAATCTGTTTCTAGCGATGGGTATTTTTTGATCTATGTTACTGCTGGTGTTTATGAAGAATATGTTACTATTGATAAGAAAAAGACTTTTTTGATGATGATTGGAGATGGTATTAACAAGACTATTATCACTGGCAATCACAGTGTTGTTGATGGCTGGACCACATTCGGCTCCCCAACATTCGCTGTGGTTGGGCAAGGATTCGTGGGTGTGAACATGACCATTCGTAACACAGCAGGTGCTGTGAAGCACCAAGCAGTGGCACTTAGAAACGGGGCAGATTTATCAACATTTTACAGTTGCAGTTTCGAAGGGTACCAAGACACGTTATACACACATTCACTAAGACAATTCTACAGAGAATGTGACATTTATGGAACAGTAGATTTCATATTCGGAAATGCTAAGGTTGTTTTCCAAAATTGTAACCTCTATCCACGTCTTCCAATGAGTGGACAATTCAATGCAATCACTGCACAAGGAAGAACAGATCCTAACCAAGACACAGGTACATCAATCCACAACTGTACAATTAAAGCAACCGATGATTTAGCTTCTAGCAATGGTGCTGTTTCAACATATTTGGGAAGACCATGGAAAGAGTATTCTAGAACGGTTTACATGCAAACATTTATGGATAATGTGATTAATGTTGCTGGGTGGCGTGCTTGGGATGGAGAATTTGCATTGAGCACATTGTATTATGCTGAGTTTAATAATACTGGGCCAGGCTCTAGCACTGATGGTAGAGTGACGTGGCAAGGCTATCGTGTGATTAATGCCACGGATGCAGCTAATTTTACGGTGGCAAATTTCTTGCTTGGTGATGATTGGTTGCCTCAAACAGGGGTGTCTTACACTAATTCCTTAATTAATTAA
Protein sequence

>Msa1366480

MASKLCNTLSIFLILLSVSIAFSQNTGTSPNPITPISPGAACKSTPDPTYCKSILPPQKANVYDYGRFSVKKSLSQSRQFSNLINKYLQRRSTLSTTAVRALQDCQSLSDLNSDFLSNSFQTVNKTTKFLPSLQGENIQTLLSAILTNQQTCLDGLKDTSSAWSFRNGLTIPLSNDTKLYSVSLAFFTKGWVNPKTNKTAFQNSKHSNKGFKNGRLPLKMTSKTRAIYESVSRRKLLQSDQVGEDVVVRDIVTVSQDGSGNFTTINDAIAAAPNKSVSSDGYFLIYVTAGVYEEYVTIDKKKTFLMMIGDGINKTIITGNHSVVDGWTTFGSPTFAVVGQGFVGVNMTIRNTAGAVKHQAVALRNGADLSTFYSCSFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAKVVFQNCNLYPRLPMSGQFNAITAQGRTDPNQDTGTSIHNCTIKATDDLASSNGAVSTYLGRPWKEYSRTVYMQTFMDNVINVAGWRAWDGEFALSTLYYAEFNNTGPGSSTDGRVTWQGYRVINATDAANFTVANFLLGDDWLPQTGVSYTNSLIN*