AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0300280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0300280 MtrunA17_Chr2g0325091 99.535 215 1 0 1 215 1 215 5.61e-159 438
Msa0300280 MtrunA17_Chr4g0046261 81.019 216 38 2 1 213 1 216 1.10e-128 361
Msa0300280 MtrunA17_Chr2g0283131 80.556 216 39 2 1 213 1 216 1.79e-128 361
Msa0300280 MtrunA17_Chr4g0040781 81.019 216 38 2 1 213 1 216 2.83e-128 360
Msa0300280 MtrunA17_Chr2g0315551 76.415 212 47 3 5 214 7 217 5.20e-118 334
Msa0300280 MtrunA17_Chr2g0319951 72.170 212 55 2 5 213 7 217 7.18e-108 308
Msa0300280 MtrunA17_Chr3g0112681 67.453 212 66 2 4 213 5 215 2.83e-102 294
Msa0300280 MtrunA17_Chr2g0276941 66.038 212 69 1 5 213 9 220 1.77e-101 293
Msa0300280 MtrunA17_Chr4g0032201 67.136 213 66 3 4 214 5 215 6.00e-101 291
Msa0300280 MtrunA17_Chr1g0174281 58.065 217 85 2 2 212 6 222 2.28e-89 262
Msa0300280 MtrunA17_Chr6g0482721 58.768 211 84 3 5 213 6 215 1.72e-87 257
Msa0300280 MtrunA17_Chr3g0123821 55.814 215 91 1 2 212 7 221 4.12e-86 254
Msa0300280 MtrunA17_Chr8g0381841 56.881 218 89 2 2 214 8 225 1.42e-85 252
Msa0300280 MtrunA17_Chr7g0231131 55.814 215 90 2 2 212 6 219 2.20e-85 252
Msa0300280 MtrunA17_Chr8g0346421 55.140 214 93 1 4 214 19 232 6.56e-81 241
Msa0300280 MtrunA17_Chr7g0275991 56.338 213 88 3 6 213 15 227 7.73e-81 241
Msa0300280 MtrunA17_Chr8g0392931 56.604 212 88 2 5 213 8 218 1.70e-79 237
Msa0300280 MtrunA17_Chr7g0214241 58.937 207 82 2 10 213 14 220 4.80e-79 236
Msa0300280 MtrunA17_Chr1g0189361 55.189 212 88 2 9 213 12 223 6.90e-77 230
Msa0300280 MtrunA17_Chr8g0351861 60.221 181 71 1 2 181 6 186 1.88e-75 227
Msa0300280 MtrunA17_Chr7g0234201 56.373 204 89 0 10 213 12 215 5.51e-75 225
Msa0300280 MtrunA17_Chr2g0290891 46.009 213 104 2 8 213 3 211 1.56e-61 191
Msa0300280 MtrunA17_Chr6g0458081 43.868 212 110 2 8 213 3 211 1.83e-58 183
Msa0300280 MtrunA17_Chr4g0055651 45.498 211 106 1 4 214 1 202 3.16e-58 182
Msa0300280 MtrunA17_Chr7g0250531 45.498 211 106 1 4 214 1 202 5.13e-57 179
Msa0300280 MtrunA17_Chr8g0335471 46.919 211 104 2 4 214 1 203 5.77e-57 179
Msa0300280 MtrunA17_Chr8g0349711 46.154 208 108 2 10 213 5 212 1.47e-54 173
Msa0300280 MtrunA17_Chr2g0276931 44.928 207 104 2 7 212 5 202 3.94e-54 172
Msa0300280 MtrunA17_Chr8g0349681 46.698 212 99 3 8 213 381 584 7.09e-52 176
Msa0300280 MtrunA17_Chr8g0349681 46.988 166 88 0 8 173 172 337 7.78e-46 159
Msa0300280 MtrunA17_Chr8g0349681 50.641 156 77 0 10 165 12 167 9.95e-46 159
Msa0300280 MtrunA17_Chr4g0030911 43.602 211 106 4 7 212 11 213 9.06e-52 166
Msa0300280 MtrunA17_Chr1g0182301 42.453 212 112 3 7 214 11 216 1.06e-51 166
Msa0300280 MtrunA17_Chr7g0263071 42.991 214 108 4 7 214 11 216 1.90e-51 165
Msa0300280 MtrunA17_Chr1g0212521 42.308 208 114 3 7 212 11 214 5.41e-51 164
Msa0300280 MtrunA17_Chr1g0182321 41.981 212 113 3 7 214 11 216 5.70e-51 164
Msa0300280 MtrunA17_Chr8g0384311 46.190 210 101 7 6 212 8 208 1.98e-49 160
Msa0300280 MtrunA17_Chr2g0327911 40.826 218 109 5 3 212 7 212 6.91e-48 156
Msa0300280 MtrunA17_Chr1g0159481 45.455 209 105 6 7 213 13 214 8.44e-48 156
Msa0300280 MtrunA17_Chr1g0199331 44.720 161 88 1 13 172 33 193 1.86e-45 149
Msa0300280 MtrunA17_Chr3g0130271 43.981 216 104 8 3 212 4 208 3.20e-45 149
Msa0300280 MtrunA17_Chr3g0109721 43.125 160 91 0 13 172 12 171 7.41e-45 148
Msa0300280 MtrunA17_Chr5g0433561 42.500 160 92 0 13 172 12 171 1.49e-43 145
Msa0300280 MtrunA17_Chr7g0265001 42.788 208 111 4 8 213 13 214 9.04e-43 143
Msa0300280 MtrunA17_Chr1g0197331 42.453 212 111 5 5 213 9 212 7.74e-42 140
Msa0300280 MtrunA17_Chr1g0180291 43.478 207 108 5 8 212 11 210 9.86e-42 140
Msa0300280 MtrunA17_Chr3g0123971 33.333 195 112 1 12 206 10 186 7.78e-39 133
Msa0300280 MtrunA17_Chr8g0390561 39.375 160 97 0 13 172 11 170 1.37e-38 132
Msa0300280 MtrunA17_Chr4g0055081 33.178 214 113 3 12 213 10 205 1.52e-38 132
Msa0300280 MtrunA17_Chr5g0400131 34.694 196 109 2 12 206 10 187 3.66e-38 131
Msa0300280 MtrunA17_Chr8g0382221 35.057 174 113 0 12 185 10 183 5.22e-38 130
Msa0300280 MtrunA17_Chr6g0466301 40.000 165 92 3 11 168 8 172 2.50e-37 129
Msa0300280 MtrunA17_Chr6g0465491 40.000 165 92 3 11 168 8 172 2.50e-37 129
Msa0300280 MtrunA17_Chr1g0201121 40.964 166 89 4 11 168 8 172 4.65e-36 125
Msa0300280 MtrunA17_Chr8g0383261 39.759 166 91 4 11 168 12 176 3.74e-35 123
Msa0300280 MtrunA17_Chr4g0034871 34.928 209 116 7 11 211 8 204 2.01e-34 121
Msa0300280 MtrunA17_Chr2g0331691 39.286 168 94 4 11 171 10 176 2.79e-34 121
Msa0300280 MtrunA17_Chr4g0004661 39.506 162 91 3 11 165 64 225 1.44e-32 118
Msa0300280 MtrunA17_Chr6g0466161 36.667 150 88 3 26 168 5 154 1.11e-24 96.7
Msa0300280 MtrunA17_Chr6g0465471 36.667 150 88 3 26 168 5 154 1.12e-24 96.7
Msa0300280 MtrunA17_Chr3g0138121 32.258 155 102 2 12 166 14 165 8.87e-24 94.4
Msa0300280 MtrunA17_Chr3g0138111 32.258 155 102 2 12 166 14 165 1.07e-23 94.4
Msa0300280 MtrunA17_Chr3g0138101 32.258 155 102 2 12 166 14 165 1.08e-23 94.4
Msa0300280 MtrunA17_Chr4g0046211 37.500 120 72 3 11 129 6 123 4.38e-17 75.1
Msa0300280 MtrunA17_Chr4g0037021 31.282 195 115 7 11 190 6 196 4.97e-17 75.9
Msa0300280 MtrunA17_Chr5g0405791 37.500 120 72 3 11 129 6 123 5.24e-17 75.9
Msa0300280 MtrunA17_Chr2g0325101 37.500 120 72 3 11 129 6 123 1.17e-16 75.1
Msa0300280 MtrunA17_Chr6g0466431 35.354 99 55 1 11 109 8 97 1.84e-16 72.0
Msa0300280 MtrunA17_Chr6g0465611 35.354 99 55 1 11 109 8 97 1.84e-16 72.0
Msa0300280 MtrunA17_Chr8g0371501 37.500 120 72 3 11 129 6 123 1.91e-16 74.3
Msa0300280 MtrunA17_Chr3g0117931 31.138 167 101 5 11 165 8 172 3.29e-16 74.3
Msa0300280 MtrunA17_Chr6g0486041 36.364 121 74 3 11 130 6 124 8.01e-16 73.2
Msa0300280 MtrunA17_Chr1g0182951 69.048 42 13 0 120 161 1 42 8.68e-14 63.5
Msa0300280 MtrunA17_Chr1g0178291 31.737 167 101 5 8 171 428 584 2.37e-13 68.2
Msa0300280 MtrunA17_Chr5g0443631 24.852 169 121 2 12 175 105 272 1.20e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0300280 AT5G60860.1 81.429 210 38 1 5 213 7 216 8.61e-127 357
Msa0300280 AT3G15060.1 78.199 211 45 1 5 214 7 217 4.91e-123 347
Msa0300280 AT4G18430.1 76.852 216 47 2 1 213 1 216 3.38e-122 345
Msa0300280 AT1G28550.1 76.498 217 47 3 1 213 1 217 7.46e-122 344
Msa0300280 AT2G33870.1 76.415 212 48 2 5 214 7 218 1.02e-119 339
Msa0300280 AT5G45750.1 77.619 210 45 2 5 213 7 215 1.30e-118 336
Msa0300280 AT4G18800.1 77.143 210 44 2 5 213 7 213 5.30e-117 332
Msa0300280 AT1G16920.1 73.460 211 54 2 4 213 6 215 8.28e-113 322
Msa0300280 AT1G06400.1 73.333 210 54 2 5 213 7 215 8.47e-113 322
Msa0300280 AT1G09630.1 65.728 213 70 2 5 215 6 217 1.20e-103 298
Msa0300280 AT1G07410.1 64.623 212 72 2 4 214 5 214 2.47e-100 290
Msa0300280 AT5G59150.1 64.186 215 71 3 4 214 5 217 9.70e-96 278
Msa0300280 AT3G46830.1 63.721 215 72 3 4 214 5 217 1.42e-95 278
Msa0300280 AT3G46830.2 63.721 215 72 3 4 214 5 217 1.42e-95 278
Msa0300280 AT5G47960.1 58.796 216 83 3 5 215 9 223 7.34e-88 258
Msa0300280 AT3G12160.1 57.209 215 87 2 2 212 6 219 1.53e-87 258
Msa0300280 AT4G39990.1 57.143 210 87 1 9 215 15 224 6.81e-85 251
Msa0300280 AT5G65270.1 55.708 219 91 2 2 214 8 226 1.69e-84 250
Msa0300280 AT5G47520.1 56.542 214 89 1 5 214 8 221 4.11e-82 244
Msa0300280 AT1G18200.1 53.812 223 86 3 5 212 7 227 6.47e-80 239
Msa0300280 AT1G05810.1 58.373 209 83 2 9 214 10 217 1.54e-78 234
Msa0300280 AT1G73640.1 55.556 207 85 2 5 204 7 213 3.92e-78 234
Msa0300280 AT1G05810.2 58.373 209 83 2 9 214 56 263 4.11e-78 235
Msa0300280 AT3G07410.1 57.407 216 89 2 1 213 1 216 5.05e-78 233
Msa0300280 AT2G31680.1 55.714 210 90 1 9 215 10 219 7.49e-78 233
Msa0300280 AT2G43130.1 58.173 208 80 2 9 213 10 213 8.54e-78 233
Msa0300280 AT1G01200.1 55.093 216 91 4 4 213 21 236 2.92e-77 232
Msa0300280 AT5G59150.2 58.434 166 63 3 53 214 3 166 5.75e-63 193
Msa0300280 AT4G17170.1 46.009 213 104 2 8 213 3 211 1.21e-61 191
Msa0300280 AT4G35860.1 44.976 209 112 1 8 213 3 211 2.23e-59 186
Msa0300280 AT4G17160.1 45.411 207 106 2 8 212 3 204 5.23e-58 182
Msa0300280 AT1G02130.1 46.445 211 105 3 4 214 1 203 9.90e-57 179
Msa0300280 AT4G17530.1 46.445 211 104 3 4 214 1 202 2.16e-55 175
Msa0300280 AT5G47200.1 45.116 215 101 3 4 214 1 202 4.37e-55 174
Msa0300280 AT3G11730.1 44.712 208 105 3 7 212 4 203 1.61e-53 171
Msa0300280 AT5G03520.1 45.755 212 102 4 7 213 11 214 6.93e-53 169
Msa0300280 AT3G46060.2 43.458 214 107 4 7 214 11 216 4.43e-51 165
Msa0300280 AT3G46060.3 43.458 214 107 4 7 214 11 216 4.43e-51 165
Msa0300280 AT3G46060.1 43.458 214 107 4 7 214 11 216 4.43e-51 165
Msa0300280 AT3G53610.2 42.523 214 109 4 7 214 11 216 1.21e-50 164
Msa0300280 AT3G53610.3 42.523 214 109 4 7 214 11 216 1.21e-50 164
Msa0300280 AT3G53610.1 42.523 214 109 4 7 214 11 216 1.21e-50 164
Msa0300280 AT3G09900.1 43.925 214 105 4 7 213 11 216 1.29e-50 164
Msa0300280 AT5G59840.1 42.523 214 109 4 7 214 11 216 5.14e-50 162
Msa0300280 AT5G45130.1 40.299 201 108 4 13 213 12 200 5.63e-47 154
Msa0300280 AT1G43890.3 47.701 174 88 3 6 177 8 180 4.53e-46 152
Msa0300280 AT1G43890.1 47.701 174 88 3 6 177 8 180 4.53e-46 152
Msa0300280 AT1G43890.2 47.701 174 88 3 6 177 8 180 4.53e-46 152
Msa0300280 AT3G54840.1 47.134 157 82 1 13 168 35 191 6.28e-46 151
Msa0300280 AT3G54840.3 46.795 156 82 1 14 168 2 157 1.71e-45 149
Msa0300280 AT5G45130.2 39.698 199 108 4 15 213 22 208 2.60e-45 150
Msa0300280 AT3G54840.2 47.403 154 80 1 13 165 35 188 3.66e-45 149
Msa0300280 AT4G19640.1 42.331 163 94 0 13 175 12 174 4.25e-45 149
Msa0300280 AT5G03530.2 44.231 208 107 5 8 213 10 210 2.50e-44 147
Msa0300280 AT5G03530.1 44.231 208 107 5 8 213 10 210 2.50e-44 147
Msa0300280 AT4G35860.2 41.463 164 93 1 53 213 2 165 2.00e-40 136
Msa0300280 AT5G64990.1 37.267 161 101 0 12 172 8 168 2.54e-38 132
Msa0300280 AT2G44610.1 33.333 216 112 3 12 213 10 207 5.78e-38 131
Msa0300280 AT2G22290.1 33.168 202 130 2 12 213 10 206 5.81e-38 131
Msa0300280 AT1G22740.1 42.515 167 85 5 11 168 8 172 9.83e-38 130
Msa0300280 AT5G03520.2 42.197 173 87 4 46 213 40 204 1.73e-37 130
Msa0300280 AT5G10260.1 36.943 157 99 0 12 168 10 166 1.01e-36 128
Msa0300280 AT4G39890.1 36.364 165 104 1 12 175 10 174 1.36e-36 127
Msa0300280 AT4G09720.1 39.394 165 93 3 11 168 8 172 8.91e-36 125
Msa0300280 AT5G64990.2 35.714 168 101 1 12 172 8 175 1.25e-35 125
Msa0300280 AT3G09910.3 42.781 187 100 4 8 192 10 191 3.47e-35 124
Msa0300280 AT3G09910.1 42.781 187 100 4 8 192 10 191 3.47e-35 124
Msa0300280 AT3G09910.2 42.781 187 100 4 8 192 10 191 3.47e-35 124
Msa0300280 AT4G19640.2 43.077 130 74 0 13 142 12 141 3.70e-35 122
Msa0300280 AT5G46025.1 58.491 106 43 1 73 177 6 111 3.86e-35 120
Msa0300280 AT2G21880.1 40.000 165 92 3 11 168 9 173 4.32e-35 124
Msa0300280 AT1G52280.1 40.237 169 91 5 11 171 8 174 1.02e-34 122
Msa0300280 AT4G19640.3 42.748 131 75 0 13 143 12 142 1.12e-34 122
Msa0300280 AT4G09720.4 38.824 170 92 4 11 168 8 177 3.34e-34 121
Msa0300280 AT3G18820.1 39.506 162 91 3 11 165 8 169 1.42e-33 119
Msa0300280 AT3G09910.5 43.114 167 92 3 8 172 10 175 1.88e-33 119
Msa0300280 AT3G09910.4 43.114 167 92 3 8 172 10 175 1.88e-33 119
Msa0300280 AT4G09720.3 37.500 176 92 4 11 168 8 183 3.56e-33 119
Msa0300280 AT3G16100.1 39.264 163 90 4 11 165 8 169 6.45e-33 118
Msa0300280 AT1G49300.2 37.725 167 97 3 11 170 8 174 1.97e-32 117
Msa0300280 AT1G49300.1 37.725 167 97 3 11 170 8 174 1.97e-32 117
Msa0300280 AT5G39620.1 38.415 164 94 3 12 168 6 169 3.40e-32 116
Msa0300280 AT2G21880.4 37.838 148 85 3 28 168 1 148 2.43e-26 100
Msa0300280 AT2G21880.3 37.838 148 85 3 28 168 1 148 2.43e-26 100
Msa0300280 AT4G09720.6 39.130 138 77 3 38 168 1 138 2.55e-25 97.4
Msa0300280 AT4G09720.5 39.130 138 77 3 38 168 1 138 2.55e-25 97.4
Msa0300280 AT4G09720.2 39.130 138 77 3 38 168 1 138 2.55e-25 97.4
Msa0300280 AT2G21880.2 35.152 165 92 4 11 168 9 165 3.71e-25 98.2
Msa0300280 AT5G20020.1 32.258 155 102 2 12 166 14 165 1.30e-23 94.4
Msa0300280 AT5G55190.1 32.258 155 102 2 12 166 14 165 1.46e-23 94.4
Msa0300280 AT5G20010.1 32.258 155 102 2 12 166 14 165 1.54e-23 94.4
Msa0300280 AT5G55080.1 30.435 161 109 2 12 172 14 171 2.76e-23 93.6
Msa0300280 AT2G44690.1 32.222 180 108 5 11 178 18 195 2.40e-19 82.8
Msa0300280 AT4G08190.1 50.704 71 32 1 95 165 60 127 6.70e-18 77.0
Msa0300280 AT4G35020.1 34.667 150 88 4 11 159 6 146 2.75e-17 77.0
Msa0300280 AT4G35020.2 34.667 150 88 4 11 159 6 146 2.75e-17 77.0
Msa0300280 AT4G35020.3 34.667 150 88 4 11 159 6 146 2.75e-17 77.0
Msa0300280 AT1G75840.1 37.500 120 72 3 11 129 6 123 1.78e-16 75.1
Msa0300280 AT1G20090.1 36.667 120 73 3 11 129 5 122 2.94e-16 74.3
Msa0300280 AT3G51300.1 38.136 118 70 3 13 129 8 123 3.60e-16 73.9
Msa0300280 AT2G17800.2 37.500 120 72 3 11 129 6 123 3.60e-16 73.9
Msa0300280 AT2G17800.1 37.500 120 72 3 11 129 6 123 3.60e-16 73.9
Msa0300280 AT4G35950.1 37.500 120 72 3 11 129 6 123 3.75e-16 73.9
Msa0300280 AT3G48040.1 31.737 167 100 5 11 165 8 172 5.07e-16 73.9
Msa0300280 AT4G28950.1 36.667 120 73 3 11 129 6 123 6.96e-16 73.6
Msa0300280 AT5G45970.1 36.667 120 73 3 11 129 6 123 8.40e-16 73.2
Msa0300280 AT5G62880.1 31.461 178 104 6 11 172 8 183 1.25e-15 73.2
Msa0300280 AT3G48040.2 36.667 120 73 3 11 129 8 125 2.56e-15 71.2
Msa0300280 AT5G62880.2 35.833 120 74 3 11 129 8 125 5.85e-15 70.9
Msa0300280 AT4G35020.4 36.066 122 75 3 13 133 8 127 3.39e-14 67.8
Msa0300280 AT4G35020.5 36.066 122 75 3 13 133 8 127 3.39e-14 67.8

Find 45 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGTAAATTCTCATCATTT+TGG 0.200341 2_3:+55642593 Msa0300280:three_prime_UTR
CATTATTGTTGTTACAATTC+AGG 0.219910 2_3:+55642754 Msa0300280:three_prime_UTR
TTCAACGCCGATGGTGGATT+TGG 0.266153 2_3:-55640883 None:intergenic
TGGAGCTGTTGGCGCTTTGC+TGG 0.307634 2_3:+55642143 Msa0300280:CDS
CTGAATTGTAACAACAATAA+TGG 0.324342 2_3:-55642753 None:intergenic
GCTTGTTAAGGCTCAGATTT+GGG 0.349305 2_3:+55640938 Msa0300280:CDS
AGCTTGTTAAGGCTCAGATT+TGG 0.356509 2_3:+55640937 Msa0300280:CDS
GAAAGAAAACATATATTTCA+TGG 0.359463 2_3:+55642329 Msa0300280:CDS
TAATGACTCCCACAACAAAT+AGG 0.380253 2_3:-55642675 None:intergenic
AAAATTCGATGGTAAAATAA+AGG 0.393287 2_3:-55642650 None:intergenic
GATTCTTGATAAGCTTGTTA+AGG 0.421134 2_3:+55640926 Msa0300280:CDS
AAGGGAGAGACTATCAATAT+TGG 0.429829 2_3:+55642471 Msa0300280:CDS
CACTAGAAATGAATTCAACT+TGG 0.437767 2_3:+55640857 Msa0300280:CDS
AAATATCAAAACATTTCAAA+TGG 0.447075 2_3:-55642619 None:intergenic
AATTTGCTACTCGAAGTGTT+AGG 0.449148 2_3:+55640904 Msa0300280:CDS
TGGCTCTGCATCAGTGCCTA+AGG 0.468622 2_3:+55642452 Msa0300280:CDS
TATGTTGTAATCATGCTTGT+AGG 0.477521 2_3:+55642243 Msa0300280:CDS
TTGATCGGTGATTCTGGTGT+TGG 0.493431 2_3:+55640810 Msa0300280:CDS
AGTGAATCTAGATAAAAGAT+TGG 0.496154 2_3:-55640838 None:intergenic
TTCTTTCTCAGCAAATGTGG+TGG 0.512980 2_3:-55642313 None:intergenic
AAGACTCTTGAGAAAGAGAA+TGG 0.519287 2_3:+55642432 Msa0300280:CDS
AGCAAATTCAACGCCGATGG+TGG 0.527864 2_3:-55640889 None:intergenic
GTTTCAGATGTTAAGAAGAG+TGG 0.531135 2_3:+55642504 Msa0300280:CDS
GGGATACTGCTGGTCAAGAA+AGG 0.539209 2_3:+55640958 Msa0300280:CDS
ATAATGGATTCTCGAGATTG+AGG 0.540347 2_3:-55642737 None:intergenic
ATACGATTACTTGTTCAAGT+TGG 0.541653 2_3:+55640785 Msa0300280:CDS
AAAGATGTATACATTAGAAG+AGG 0.552062 2_3:+55642556 Msa0300280:three_prime_UTR
AAATGTTGAGAGGTGGTTGA+AGG 0.552257 2_3:+55642197 Msa0300280:CDS
ATATTGATAGTCTCTCCCTT+AGG 0.560011 2_3:-55642468 None:intergenic
GCATACTACCGTGGAGCTGT+TGG 0.560757 2_3:+55642132 Msa0300280:CDS
GGTGGCTTCTTCCGTTGACA+CGG 0.568498 2_3:-55642295 None:intergenic
GCTCAGATTTGGGATACTGC+TGG 0.571910 2_3:+55640948 Msa0300280:CDS
TACAAGCATGATTACAACAT+AGG 0.576438 2_3:-55642241 None:intergenic
TGCACTAGAGTCGTTGAATG+TGG 0.582071 2_3:+55642359 Msa0300280:CDS
GAATGTGGACAATGCTTTCG+TGG 0.585079 2_3:+55642374 Msa0300280:CDS
GGCTCTGCATCAGTGCCTAA+GGG 0.588553 2_3:+55642453 Msa0300280:CDS
ACACTTGCAAGCCGTGTCAA+CGG 0.594812 2_3:+55642284 Msa0300280:CDS
CATTTGAAAATGTTGAGAGG+TGG 0.614736 2_3:+55642190 Msa0300280:CDS
AGTAGCAAATTCAACGCCGA+TGG 0.615075 2_3:-55640892 None:intergenic
TTGGAATCCAAATCCACCAT+CGG 0.626401 2_3:+55640876 Msa0300280:CDS
TGCTGGTGTACGACTCAACA+AGG 0.627995 2_3:+55642160 Msa0300280:CDS
GCAAAGCGCCAACAGCTCCA+CGG 0.659685 2_3:-55642140 None:intergenic
ACAAATAGGAGAAAATTCGA+TGG 0.662032 2_3:-55642661 None:intergenic
TGACATTTGAAAATGTTGAG+AGG 0.673555 2_3:+55642187 Msa0300280:CDS
ATCACAAGTGCATACTACCG+TGG 0.721157 2_3:+55642123 Msa0300280:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAACTTGTTATATTGTTTT+TGG + chr2_3:55641704-55641723 Msa0300280:intron 15.0%
!! AAATATCAAAACATTTCAAA+TGG - chr2_3:55642622-55642641 None:intergenic 15.0%
!!! TAAACTTGTTATATTGTTTT+TGG + chr2_3:55641704-55641723 Msa0300280:intron 15.0%
!! AAATATCAAAACATTTCAAA+TGG - chr2_3:55642622-55642641 None:intergenic 15.0%
!!! TACTCTTTCATTTTTAATGT+CGG + chr2_3:55641343-55641362 Msa0300280:intron 20.0%
!! ATATTGGAAGAAATAAACAA+TGG - chr2_3:55641508-55641527 None:intergenic 20.0%
!!! CAAATTTTGAAAAAGCTAAA+TGG - chr2_3:55641531-55641550 None:intergenic 20.0%
!! GTAATTTCTTCAATATATCA+AGG + chr2_3:55641550-55641569 Msa0300280:intron 20.0%
!!! TGAGATTATTTTAACTTTAG+AGG - chr2_3:55641738-55641757 None:intergenic 20.0%
!!! AAATGGAATTCTCATTTTAT+AGG - chr2_3:55641980-55641999 None:intergenic 20.0%
!! GAAAGAAAACATATATTTCA+TGG + chr2_3:55642329-55642348 Msa0300280:CDS 20.0%
!! AAAATTCGATGGTAAAATAA+AGG - chr2_3:55642653-55642672 None:intergenic 20.0%
!!! TACTCTTTCATTTTTAATGT+CGG + chr2_3:55641343-55641362 Msa0300280:intron 20.0%
!! ATATTGGAAGAAATAAACAA+TGG - chr2_3:55641508-55641527 None:intergenic 20.0%
!!! CAAATTTTGAAAAAGCTAAA+TGG - chr2_3:55641531-55641550 None:intergenic 20.0%
!! GTAATTTCTTCAATATATCA+AGG + chr2_3:55641550-55641569 Msa0300280:intron 20.0%
!!! TGAGATTATTTTAACTTTAG+AGG - chr2_3:55641738-55641757 None:intergenic 20.0%
!!! AAATGGAATTCTCATTTTAT+AGG - chr2_3:55641980-55641999 None:intergenic 20.0%
!! GAAAGAAAACATATATTTCA+TGG + chr2_3:55642329-55642348 Msa0300280:CDS 20.0%
!! AAAATTCGATGGTAAAATAA+AGG - chr2_3:55642653-55642672 None:intergenic 20.0%
! GATCGAAAAATGAATGAAAA+GGG - chr2_3:55640712-55640731 None:intergenic 25.0%
! AGATCGAAAAATGAATGAAA+AGG - chr2_3:55640713-55640732 None:intergenic 25.0%
! AGTGAATCTAGATAAAAGAT+TGG - chr2_3:55640841-55640860 None:intergenic 25.0%
! AGAATGAAATTGAAGAGAAA+AGG - chr2_3:55640989-55641008 None:intergenic 25.0%
! GATTGATGAATTGAATTGAA+TGG + chr2_3:55641185-55641204 Msa0300280:intron 25.0%
!!! TACTTTTTTGCTTTTGATCT+TGG + chr2_3:55641229-55641248 Msa0300280:intron 25.0%
!!! TGTATCTTAGAATTTGTTGT+TGG + chr2_3:55641283-55641302 Msa0300280:intron 25.0%
!!! GTATCTTAGAATTTGTTGTT+GGG + chr2_3:55641284-55641303 Msa0300280:intron 25.0%
!!! ACTCTTTCATTTTTAATGTC+GGG + chr2_3:55641344-55641363 Msa0300280:intron 25.0%
! GTTCAATCTGTTGTTAAATA+AGG + chr2_3:55641367-55641386 Msa0300280:intron 25.0%
! TGATATGATCACAAAAACTA+TGG + chr2_3:55641468-55641487 Msa0300280:intron 25.0%
! TGAAAAAGCTAAATGGATAT+TGG - chr2_3:55641524-55641543 None:intergenic 25.0%
!! AAATTGAACTCTCATTTTAG+AGG - chr2_3:55641863-55641882 None:intergenic 25.0%
! AAAGATGTATACATTAGAAG+AGG + chr2_3:55642556-55642575 Msa0300280:three_prime_UTR 25.0%
!! AGAGTAAATTCTCATCATTT+TGG + chr2_3:55642593-55642612 Msa0300280:three_prime_UTR 25.0%
!! GAGTAAATTCTCATCATTTT+GGG + chr2_3:55642594-55642613 Msa0300280:three_prime_UTR 25.0%
!!! AATTTTCTCCTATTTGTTGT+GGG + chr2_3:55642667-55642686 Msa0300280:three_prime_UTR 25.0%
! CTGAATTGTAACAACAATAA+TGG - chr2_3:55642756-55642775 None:intergenic 25.0%
!! CATTATTGTTGTTACAATTC+AGG + chr2_3:55642754-55642773 Msa0300280:three_prime_UTR 25.0%
! GATCGAAAAATGAATGAAAA+GGG - chr2_3:55640712-55640731 None:intergenic 25.0%
! AGATCGAAAAATGAATGAAA+AGG - chr2_3:55640713-55640732 None:intergenic 25.0%
! AGTGAATCTAGATAAAAGAT+TGG - chr2_3:55640841-55640860 None:intergenic 25.0%
! AGAATGAAATTGAAGAGAAA+AGG - chr2_3:55640989-55641008 None:intergenic 25.0%
! GATTGATGAATTGAATTGAA+TGG + chr2_3:55641185-55641204 Msa0300280:intron 25.0%
!!! TACTTTTTTGCTTTTGATCT+TGG + chr2_3:55641229-55641248 Msa0300280:intron 25.0%
!!! TGTATCTTAGAATTTGTTGT+TGG + chr2_3:55641283-55641302 Msa0300280:intron 25.0%
!!! GTATCTTAGAATTTGTTGTT+GGG + chr2_3:55641284-55641303 Msa0300280:intron 25.0%
!!! ACTCTTTCATTTTTAATGTC+GGG + chr2_3:55641344-55641363 Msa0300280:intron 25.0%
! GTTCAATCTGTTGTTAAATA+AGG + chr2_3:55641367-55641386 Msa0300280:intron 25.0%
! TGATATGATCACAAAAACTA+TGG + chr2_3:55641468-55641487 Msa0300280:intron 25.0%
! TGAAAAAGCTAAATGGATAT+TGG - chr2_3:55641524-55641543 None:intergenic 25.0%
!! AAATTGAACTCTCATTTTAG+AGG - chr2_3:55641863-55641882 None:intergenic 25.0%
! AAAGATGTATACATTAGAAG+AGG + chr2_3:55642556-55642575 Msa0300280:three_prime_UTR 25.0%
!! AGAGTAAATTCTCATCATTT+TGG + chr2_3:55642593-55642612 Msa0300280:three_prime_UTR 25.0%
!! GAGTAAATTCTCATCATTTT+GGG + chr2_3:55642594-55642613 Msa0300280:three_prime_UTR 25.0%
!!! AATTTTCTCCTATTTGTTGT+GGG + chr2_3:55642667-55642686 Msa0300280:three_prime_UTR 25.0%
! CTGAATTGTAACAACAATAA+TGG - chr2_3:55642756-55642775 None:intergenic 25.0%
!! CATTATTGTTGTTACAATTC+AGG + chr2_3:55642754-55642773 Msa0300280:three_prime_UTR 25.0%
ATCATCATCATCATCATCAA+TGG + chr2_3:55640746-55640765 Msa0300280:exon 30.0%
TCATCATCATCATCATCAAT+GGG + chr2_3:55640747-55640766 Msa0300280:exon 30.0%
ATACGATTACTTGTTCAAGT+TGG + chr2_3:55640785-55640804 Msa0300280:CDS 30.0%
!!! TTGTTCAAGTTGGTTTTGAT+CGG + chr2_3:55640795-55640814 Msa0300280:CDS 30.0%
CACTAGAAATGAATTCAACT+TGG + chr2_3:55640857-55640876 Msa0300280:CDS 30.0%
!! GATTCTTGATAAGCTTGTTA+AGG + chr2_3:55640926-55640945 Msa0300280:CDS 30.0%
!!! GCAAAAAGTGTGTTTTTTTG+TGG + chr2_3:55641043-55641062 Msa0300280:intron 30.0%
!!! AGTGTGTTTTTTTGTGGTTT+TGG + chr2_3:55641049-55641068 Msa0300280:intron 30.0%
!!! GTGTGTTTTTTTGTGGTTTT+GGG + chr2_3:55641050-55641069 Msa0300280:intron 30.0%
!! GGGTTTTGATCTTAATGTTT+AGG + chr2_3:55641070-55641089 Msa0300280:intron 30.0%
!! TGTGTCTGATAATTTGTTGT+TGG + chr2_3:55641117-55641136 Msa0300280:intron 30.0%
!! GTGTCTGATAATTTGTTGTT+GGG + chr2_3:55641118-55641137 Msa0300280:intron 30.0%
AATGGAGTGAAAATGCAAAA+GGG + chr2_3:55641203-55641222 Msa0300280:intron 30.0%
!! CTCTTTCATTTTTAATGTCG+GGG + chr2_3:55641345-55641364 Msa0300280:intron 30.0%
ATGATCACAAAAACTATGGA+TGG + chr2_3:55641472-55641491 Msa0300280:intron 30.0%
GGAAGAAATAAACAATGGTT+AGG - chr2_3:55641503-55641522 None:intergenic 30.0%
!!! GAGTTTTAGAAGAGAATCTA+GGG - chr2_3:55641604-55641623 None:intergenic 30.0%
!!! AGAGTTTTAGAAGAGAATCT+AGG - chr2_3:55641605-55641624 None:intergenic 30.0%
!! GACTTTTAGAAGAGAAACTA+TGG - chr2_3:55641670-55641689 None:intergenic 30.0%
TAAGTGCATATTTGGTATCA+CGG - chr2_3:55641834-55641853 None:intergenic 30.0%
GGAAAAAGTAAGTGCATATT+TGG - chr2_3:55641842-55641861 None:intergenic 30.0%
AAAACGAGTTTACGAGATTT+AGG - chr2_3:55641955-55641974 None:intergenic 30.0%
AGACGGAAAAATTCTCTAAA+TGG - chr2_3:55641997-55642016 None:intergenic 30.0%
TGTAAGTATCACATTGTAGA+CGG - chr2_3:55642014-55642033 None:intergenic 30.0%
TGACATTTGAAAATGTTGAG+AGG + chr2_3:55642187-55642206 Msa0300280:CDS 30.0%
TACAAGCATGATTACAACAT+AGG - chr2_3:55642244-55642263 None:intergenic 30.0%
TATGTTGTAATCATGCTTGT+AGG + chr2_3:55642243-55642262 Msa0300280:CDS 30.0%
ACAAATAGGAGAAAATTCGA+TGG - chr2_3:55642664-55642683 None:intergenic 30.0%
!! GAATTTTCTCCTATTTGTTG+TGG + chr2_3:55642666-55642685 Msa0300280:three_prime_UTR 30.0%
ATCATCATCATCATCATCAA+TGG + chr2_3:55640746-55640765 Msa0300280:exon 30.0%
TCATCATCATCATCATCAAT+GGG + chr2_3:55640747-55640766 Msa0300280:exon 30.0%
ATACGATTACTTGTTCAAGT+TGG + chr2_3:55640785-55640804 Msa0300280:CDS 30.0%
!!! TTGTTCAAGTTGGTTTTGAT+CGG + chr2_3:55640795-55640814 Msa0300280:CDS 30.0%
CACTAGAAATGAATTCAACT+TGG + chr2_3:55640857-55640876 Msa0300280:CDS 30.0%
!! GATTCTTGATAAGCTTGTTA+AGG + chr2_3:55640926-55640945 Msa0300280:CDS 30.0%
!!! GCAAAAAGTGTGTTTTTTTG+TGG + chr2_3:55641043-55641062 Msa0300280:intron 30.0%
!!! AGTGTGTTTTTTTGTGGTTT+TGG + chr2_3:55641049-55641068 Msa0300280:intron 30.0%
!!! GTGTGTTTTTTTGTGGTTTT+GGG + chr2_3:55641050-55641069 Msa0300280:intron 30.0%
!! GGGTTTTGATCTTAATGTTT+AGG + chr2_3:55641070-55641089 Msa0300280:intron 30.0%
!! TGTGTCTGATAATTTGTTGT+TGG + chr2_3:55641117-55641136 Msa0300280:intron 30.0%
!! GTGTCTGATAATTTGTTGTT+GGG + chr2_3:55641118-55641137 Msa0300280:intron 30.0%
AATGGAGTGAAAATGCAAAA+GGG + chr2_3:55641203-55641222 Msa0300280:intron 30.0%
!! CTCTTTCATTTTTAATGTCG+GGG + chr2_3:55641345-55641364 Msa0300280:intron 30.0%
ATGATCACAAAAACTATGGA+TGG + chr2_3:55641472-55641491 Msa0300280:intron 30.0%
GGAAGAAATAAACAATGGTT+AGG - chr2_3:55641503-55641522 None:intergenic 30.0%
!!! GAGTTTTAGAAGAGAATCTA+GGG - chr2_3:55641604-55641623 None:intergenic 30.0%
!!! AGAGTTTTAGAAGAGAATCT+AGG - chr2_3:55641605-55641624 None:intergenic 30.0%
!! GACTTTTAGAAGAGAAACTA+TGG - chr2_3:55641670-55641689 None:intergenic 30.0%
TAAGTGCATATTTGGTATCA+CGG - chr2_3:55641834-55641853 None:intergenic 30.0%
GGAAAAAGTAAGTGCATATT+TGG - chr2_3:55641842-55641861 None:intergenic 30.0%
AAAACGAGTTTACGAGATTT+AGG - chr2_3:55641955-55641974 None:intergenic 30.0%
AGACGGAAAAATTCTCTAAA+TGG - chr2_3:55641997-55642016 None:intergenic 30.0%
TGTAAGTATCACATTGTAGA+CGG - chr2_3:55642014-55642033 None:intergenic 30.0%
TGACATTTGAAAATGTTGAG+AGG + chr2_3:55642187-55642206 Msa0300280:CDS 30.0%
TACAAGCATGATTACAACAT+AGG - chr2_3:55642244-55642263 None:intergenic 30.0%
TATGTTGTAATCATGCTTGT+AGG + chr2_3:55642243-55642262 Msa0300280:CDS 30.0%
ACAAATAGGAGAAAATTCGA+TGG - chr2_3:55642664-55642683 None:intergenic 30.0%
!! GAATTTTCTCCTATTTGTTG+TGG + chr2_3:55642666-55642685 Msa0300280:three_prime_UTR 30.0%
!! AATTTGCTACTCGAAGTGTT+AGG + chr2_3:55640904-55640923 Msa0300280:CDS 35.0%
GAATGGAGTGAAAATGCAAA+AGG + chr2_3:55641202-55641221 Msa0300280:intron 35.0%
AAGACACTGATCATAAACAG+AGG + chr2_3:55641257-55641276 Msa0300280:intron 35.0%
!! AATTTGTTGTTGGGTAGAGA+AGG + chr2_3:55641127-55641146 Msa0300280:intron 35.0%
ATCACAAAAACTATGGATGG+TGG + chr2_3:55641475-55641494 Msa0300280:intron 35.0%
! AGAAGAGAATCTAGGGTAAT+TGG - chr2_3:55641597-55641616 None:intergenic 35.0%
GAAGAGAAACTATGGCTAAT+TGG - chr2_3:55641662-55641681 None:intergenic 35.0%
AATGAGAGACTACAAGATTC+AGG - chr2_3:55642075-55642094 None:intergenic 35.0%
CATTTGAAAATGTTGAGAGG+TGG + chr2_3:55642190-55642209 Msa0300280:CDS 35.0%
! GTTTTCTTTCTCAGCAAATG+TGG - chr2_3:55642319-55642338 None:intergenic 35.0%
AAGACTCTTGAGAAAGAGAA+TGG + chr2_3:55642432-55642451 Msa0300280:CDS 35.0%
! ATATTGATAGTCTCTCCCTT+AGG - chr2_3:55642471-55642490 None:intergenic 35.0%
AAGGGAGAGACTATCAATAT+TGG + chr2_3:55642471-55642490 Msa0300280:CDS 35.0%
GTTTCAGATGTTAAGAAGAG+TGG + chr2_3:55642504-55642523 Msa0300280:CDS 35.0%
TAATGACTCCCACAACAAAT+AGG - chr2_3:55642678-55642697 None:intergenic 35.0%
!! GTTTTCGTTTGCTGAGATAT+AGG + chr2_3:55642709-55642728 Msa0300280:three_prime_UTR 35.0%
!! ATAATGGATTCTCGAGATTG+AGG - chr2_3:55642740-55642759 None:intergenic 35.0%
!! AATTTGCTACTCGAAGTGTT+AGG + chr2_3:55640904-55640923 Msa0300280:CDS 35.0%
GAATGGAGTGAAAATGCAAA+AGG + chr2_3:55641202-55641221 Msa0300280:intron 35.0%
AAGACACTGATCATAAACAG+AGG + chr2_3:55641257-55641276 Msa0300280:intron 35.0%
!! AATTTGTTGTTGGGTAGAGA+AGG + chr2_3:55641127-55641146 Msa0300280:intron 35.0%
ATCACAAAAACTATGGATGG+TGG + chr2_3:55641475-55641494 Msa0300280:intron 35.0%
! AGAAGAGAATCTAGGGTAAT+TGG - chr2_3:55641597-55641616 None:intergenic 35.0%
GAAGAGAAACTATGGCTAAT+TGG - chr2_3:55641662-55641681 None:intergenic 35.0%
AATGAGAGACTACAAGATTC+AGG - chr2_3:55642075-55642094 None:intergenic 35.0%
CATTTGAAAATGTTGAGAGG+TGG + chr2_3:55642190-55642209 Msa0300280:CDS 35.0%
! GTTTTCTTTCTCAGCAAATG+TGG - chr2_3:55642319-55642338 None:intergenic 35.0%
AAGACTCTTGAGAAAGAGAA+TGG + chr2_3:55642432-55642451 Msa0300280:CDS 35.0%
! ATATTGATAGTCTCTCCCTT+AGG - chr2_3:55642471-55642490 None:intergenic 35.0%
AAGGGAGAGACTATCAATAT+TGG + chr2_3:55642471-55642490 Msa0300280:CDS 35.0%
GTTTCAGATGTTAAGAAGAG+TGG + chr2_3:55642504-55642523 Msa0300280:CDS 35.0%
TAATGACTCCCACAACAAAT+AGG - chr2_3:55642678-55642697 None:intergenic 35.0%
!! GTTTTCGTTTGCTGAGATAT+AGG + chr2_3:55642709-55642728 Msa0300280:three_prime_UTR 35.0%
!! ATAATGGATTCTCGAGATTG+AGG - chr2_3:55642740-55642759 None:intergenic 35.0%
!!! TTGGTTTTGATCGGTGATTC+TGG + chr2_3:55640804-55640823 Msa0300280:CDS 40.0%
TTGGAATCCAAATCCACCAT+CGG + chr2_3:55640876-55640895 Msa0300280:CDS 40.0%
!! AGCTTGTTAAGGCTCAGATT+TGG + chr2_3:55640937-55640956 Msa0300280:CDS 40.0%
! GCTTGTTAAGGCTCAGATTT+GGG + chr2_3:55640938-55640957 Msa0300280:CDS 40.0%
GTTGGGTAGAGAATGAATTG+AGG + chr2_3:55641135-55641154 Msa0300280:intron 40.0%
TGTTGGGTAGAGAAGGAATT+TGG + chr2_3:55641300-55641319 Msa0300280:intron 40.0%
GTTGGGTAGAGAAGGAATTT+GGG + chr2_3:55641301-55641320 Msa0300280:intron 40.0%
AAATGTTGAGAGGTGGTTGA+AGG + chr2_3:55642197-55642216 Msa0300280:CDS 40.0%
TTCTTTCTCAGCAAATGTGG+TGG - chr2_3:55642316-55642335 None:intergenic 40.0%
!!! TTGGTTTTGATCGGTGATTC+TGG + chr2_3:55640804-55640823 Msa0300280:CDS 40.0%
TTGGAATCCAAATCCACCAT+CGG + chr2_3:55640876-55640895 Msa0300280:CDS 40.0%
!! AGCTTGTTAAGGCTCAGATT+TGG + chr2_3:55640937-55640956 Msa0300280:CDS 40.0%
! GCTTGTTAAGGCTCAGATTT+GGG + chr2_3:55640938-55640957 Msa0300280:CDS 40.0%
GTTGGGTAGAGAATGAATTG+AGG + chr2_3:55641135-55641154 Msa0300280:intron 40.0%
TGTTGGGTAGAGAAGGAATT+TGG + chr2_3:55641300-55641319 Msa0300280:intron 40.0%
GTTGGGTAGAGAAGGAATTT+GGG + chr2_3:55641301-55641320 Msa0300280:intron 40.0%
AAATGTTGAGAGGTGGTTGA+AGG + chr2_3:55642197-55642216 Msa0300280:CDS 40.0%
TTCTTTCTCAGCAAATGTGG+TGG - chr2_3:55642316-55642335 None:intergenic 40.0%
TTGATCGGTGATTCTGGTGT+TGG + chr2_3:55640810-55640829 Msa0300280:CDS 45.0%
AGTAGCAAATTCAACGCCGA+TGG - chr2_3:55640895-55640914 None:intergenic 45.0%
AAGATGTCCTGCTGCATGTT+TGG + chr2_3:55641777-55641796 Msa0300280:intron 45.0%
TGTGATACCAAACATGCAGC+AGG - chr2_3:55641787-55641806 None:intergenic 45.0%
ATCACAAGTGCATACTACCG+TGG + chr2_3:55642123-55642142 Msa0300280:CDS 45.0%
TGCACTAGAGTCGTTGAATG+TGG + chr2_3:55642359-55642378 Msa0300280:CDS 45.0%
GAATGTGGACAATGCTTTCG+TGG + chr2_3:55642374-55642393 Msa0300280:CDS 45.0%
TTGATCGGTGATTCTGGTGT+TGG + chr2_3:55640810-55640829 Msa0300280:CDS 45.0%
AGTAGCAAATTCAACGCCGA+TGG - chr2_3:55640895-55640914 None:intergenic 45.0%
AAGATGTCCTGCTGCATGTT+TGG + chr2_3:55641777-55641796 Msa0300280:intron 45.0%
TGTGATACCAAACATGCAGC+AGG - chr2_3:55641787-55641806 None:intergenic 45.0%
ATCACAAGTGCATACTACCG+TGG + chr2_3:55642123-55642142 Msa0300280:CDS 45.0%
TGCACTAGAGTCGTTGAATG+TGG + chr2_3:55642359-55642378 Msa0300280:CDS 45.0%
GAATGTGGACAATGCTTTCG+TGG + chr2_3:55642374-55642393 Msa0300280:CDS 45.0%
!! TTCAACGCCGATGGTGGATT+TGG - chr2_3:55640886-55640905 None:intergenic 50.0%
AGCAAATTCAACGCCGATGG+TGG - chr2_3:55640892-55640911 None:intergenic 50.0%
! GCTCAGATTTGGGATACTGC+TGG + chr2_3:55640948-55640967 Msa0300280:CDS 50.0%
GGGATACTGCTGGTCAAGAA+AGG + chr2_3:55640958-55640977 Msa0300280:CDS 50.0%
TGCTGGTGTACGACTCAACA+AGG + chr2_3:55642160-55642179 Msa0300280:CDS 50.0%
ACACTTGCAAGCCGTGTCAA+CGG + chr2_3:55642284-55642303 Msa0300280:CDS 50.0%
!! TTCAACGCCGATGGTGGATT+TGG - chr2_3:55640886-55640905 None:intergenic 50.0%
AGCAAATTCAACGCCGATGG+TGG - chr2_3:55640892-55640911 None:intergenic 50.0%
! GCTCAGATTTGGGATACTGC+TGG + chr2_3:55640948-55640967 Msa0300280:CDS 50.0%
GGGATACTGCTGGTCAAGAA+AGG + chr2_3:55640958-55640977 Msa0300280:CDS 50.0%
TGCTGGTGTACGACTCAACA+AGG + chr2_3:55642160-55642179 Msa0300280:CDS 50.0%
ACACTTGCAAGCCGTGTCAA+CGG + chr2_3:55642284-55642303 Msa0300280:CDS 50.0%
GCATACTACCGTGGAGCTGT+TGG + chr2_3:55642132-55642151 Msa0300280:CDS 55.0%
GGTGGCTTCTTCCGTTGACA+CGG - chr2_3:55642298-55642317 None:intergenic 55.0%
! TGGCTCTGCATCAGTGCCTA+AGG + chr2_3:55642452-55642471 Msa0300280:CDS 55.0%
GGCTCTGCATCAGTGCCTAA+GGG + chr2_3:55642453-55642472 Msa0300280:CDS 55.0%
GCATACTACCGTGGAGCTGT+TGG + chr2_3:55642132-55642151 Msa0300280:CDS 55.0%
GGTGGCTTCTTCCGTTGACA+CGG - chr2_3:55642298-55642317 None:intergenic 55.0%
! TGGCTCTGCATCAGTGCCTA+AGG + chr2_3:55642452-55642471 Msa0300280:CDS 55.0%
GGCTCTGCATCAGTGCCTAA+GGG + chr2_3:55642453-55642472 Msa0300280:CDS 55.0%
! GCAAAGCGCCAACAGCTCCA+CGG - chr2_3:55642143-55642162 None:intergenic 60.0%
TGGAGCTGTTGGCGCTTTGC+TGG + chr2_3:55642143-55642162 Msa0300280:CDS 60.0%
! GCAAAGCGCCAACAGCTCCA+CGG - chr2_3:55642143-55642162 None:intergenic 60.0%
TGGAGCTGTTGGCGCTTTGC+TGG + chr2_3:55642143-55642162 Msa0300280:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 55640659 55642798 55640659 ID=Msa0300280;Name=Msa0300280
chr2_3 mRNA 55640659 55642798 55640659 ID=Msa0300280-mRNA-1;Parent=Msa0300280;Name=Msa0300280-mRNA-1;_AED=0.05;_eAED=0.05;_QI=106|1|1|1|1|1|2|253|215
chr2_3 exon 55640659 55640979 55640659 ID=Msa0300280-mRNA-1:exon:17775;Parent=Msa0300280-mRNA-1
chr2_3 exon 55642113 55642798 55642113 ID=Msa0300280-mRNA-1:exon:17776;Parent=Msa0300280-mRNA-1
chr2_3 five_prime_UTR 55640659 55640764 55640659 ID=Msa0300280-mRNA-1:five_prime_utr;Parent=Msa0300280-mRNA-1
chr2_3 CDS 55640765 55640979 55640765 ID=Msa0300280-mRNA-1:cds;Parent=Msa0300280-mRNA-1
chr2_3 CDS 55642113 55642545 55642113 ID=Msa0300280-mRNA-1:cds;Parent=Msa0300280-mRNA-1
chr2_3 three_prime_UTR 55642546 55642798 55642546 ID=Msa0300280-mRNA-1:three_prime_utr;Parent=Msa0300280-mRNA-1
Gene Sequence

>Msa0300280

ATGGGTGCTGTAGATGAAGAATACGATTACTTGTTCAAGTTGGTTTTGATCGGTGATTCTGGTGTTGGAAAGTCCAATCTTTTATCTAGATTCACTAGAAATGAATTCAACTTGGAATCCAAATCCACCATCGGCGTTGAATTTGCTACTCGAAGTGTTAGGATTCTTGATAAGCTTGTTAAGGCTCAGATTTGGGATACTGCTGGTCAAGAAAGATATCGTGCAATCACAAGTGCATACTACCGTGGAGCTGTTGGCGCTTTGCTGGTGTACGACTCAACAAGGCATGTGACATTTGAAAATGTTGAGAGGTGGTTGAAGGAGCTTCGAGATCATACAGATGCCTATGTTGTAATCATGCTTGTAGGAAACAAAGCTGATCTTCGACACTTGCAAGCCGTGTCAACGGAAGAAGCCACCACATTTGCTGAGAAAGAAAACATATATTTCATGGAAACTTCTGCACTAGAGTCGTTGAATGTGGACAATGCTTTCGTGGAAGTGCTCACTCAGATATACAATGTTGTGAGTAAGAAGACTCTTGAGAAAGAGAATGGCTCTGCATCAGTGCCTAAGGGAGAGACTATCAATATTGGTAAAGATGATGTTTCAGATGTTAAGAAGAGTGGATGCTGCAGTACAGCATAG
Protein sequence

>Msa0300280

MGAVDEEYDYLFKLVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSVRILDKLVKAQIWDTAGQERYRAITSAYYRGAVGALLVYDSTRHVTFENVERWLKELRDHTDAYVVIMLVGNKADLRHLQAVSTEEATTFAEKENIYFMETSALESLNVDNAFVEVLTQIYNVVSKKTLEKENGSASVPKGETINIGKDDVSDVKKSGCCSTA*