AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0225210


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0225210 MtrunA17_Chr2g0324921 100.000 56 0 0 1 56 1 56 2.11e-32 115
Msa0225210 MtrunA17_Chr4g0006391 92.857 56 4 0 1 56 1 56 9.52e-30 108
Msa0225210 MtrunA17_Chr3g0138471 85.714 56 8 0 1 56 1 56 2.82e-29 106
Msa0225210 MtrunA17_Chr4g0040501 86.207 58 8 0 1 58 1 58 1.30e-28 105
Msa0225210 MtrunA17_Chr2g0282761 76.786 56 13 0 1 56 1 56 3.31e-26 99.0
Msa0225210 MtrunA17_Chr2g0282741 80.357 56 11 0 1 56 1 56 1.88e-25 97.1
Msa0225210 MtrunA17_Chr7g0263971 52.830 53 25 0 4 56 369 421 3.65e-16 72.0
Msa0225210 MtrunA17_Chr5g0415731 56.667 60 19 1 4 56 5 64 2.41e-15 66.6
Msa0225210 MtrunA17_Chr7g0271891 44.286 70 36 1 5 71 17 86 2.64e-15 67.0
Msa0225210 MtrunA17_Chr7g0269841 61.538 52 19 1 5 56 27 77 7.34e-15 68.2
Msa0225210 MtrunA17_Chr4g0021141 49.057 53 26 1 4 56 22 73 6.68e-13 60.1
Msa0225210 MtrunA17_Chr5g0427221 49.057 53 26 1 4 56 48 99 2.47e-11 56.2
Msa0225210 MtrunA17_Chr6g0478651 52.727 55 24 1 2 56 66 118 8.04e-11 57.0
Msa0225210 MtrunA17_Chr5g0414471 42.647 68 38 1 4 71 25 91 8.53e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0225210 AT3G08910.1 96.429 56 2 0 1 56 1 56 1.97e-33 117
Msa0225210 AT4G28480.2 92.857 56 4 0 1 56 1 56 8.84e-32 112
Msa0225210 AT5G01390.3 89.286 56 6 0 1 56 1 56 1.81e-31 110
Msa0225210 AT5G01390.4 89.286 56 6 0 1 56 1 56 2.23e-31 110
Msa0225210 AT4G28480.1 92.857 56 4 0 1 56 1 56 2.56e-31 112
Msa0225210 AT5G01390.2 89.286 56 6 0 1 56 1 56 4.75e-31 110
Msa0225210 AT5G01390.1 89.286 56 6 0 1 56 1 56 9.85e-31 111
Msa0225210 AT2G20560.1 89.286 56 6 0 1 56 1 56 2.98e-30 109
Msa0225210 AT5G25530.2 80.357 56 11 0 1 56 1 56 1.82e-27 102
Msa0225210 AT1G10350.1 80.357 56 11 0 1 56 1 56 2.11e-27 102
Msa0225210 AT5G25530.1 80.000 55 11 0 1 55 1 55 1.06e-26 100
Msa0225210 AT1G59725.1 75.439 57 13 1 1 56 1 57 1.23e-23 92.4
Msa0225210 AT3G47940.1 71.930 57 15 1 1 56 1 57 3.12e-21 85.9
Msa0225210 AT5G03160.1 52.830 53 25 0 4 56 370 422 3.27e-15 69.7
Msa0225210 AT1G56300.2 50.909 55 24 1 5 56 14 68 9.89e-14 62.4
Msa0225210 AT1G56300.1 50.909 55 24 1 5 56 14 68 1.19e-13 62.8
Msa0225210 AT3G62600.1 54.545 55 24 1 2 56 24 77 8.86e-13 62.8
Msa0225210 AT5G16650.1 50.943 53 25 1 4 56 11 62 1.96e-12 59.3
Msa0225210 AT5G16650.2 50.943 53 25 1 4 56 11 62 2.27e-12 59.7
Msa0225210 AT3G17830.1 56.364 55 22 1 2 56 61 113 1.32e-11 59.7
Msa0225210 AT2G41000.2 44.828 58 28 1 3 56 2 59 1.37e-11 58.5
Msa0225210 AT2G41000.1 44.828 58 28 1 3 56 2 59 2.14e-11 57.4

Find 19 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGAGATTTGCTTAAATT+TGG 0.199073 2_1:-75299996 None:intergenic
TTTCTTCAAGTCATCGTCTT+TGG 0.326757 2_1:-75299906 None:intergenic
TTGTTATTAGGGTTCTTATC+AGG 0.352145 2_1:-75299959 None:intergenic
TGACTACTACAAGCTTCTTC+AGG 0.376848 2_1:+75299871 Msa0225210:CDS
GCCGCAAAATCGCAGAAAAT+CGG 0.395524 2_1:+75299784 Msa0225210:five_prime_UTR
CTTATCAGGATGCCACTTCA+TGG 0.411951 2_1:-75299945 None:intergenic
ACAAGCTTCTTCAGGTTGAT+CGG 0.516804 2_1:+75299879 Msa0225210:CDS
AGAAGGGCAACAACCAGCCA+TGG 0.552284 2_1:+75299844 Msa0225210:exon
TAGTAGTCAACACCCATGGC+TGG 0.554236 2_1:-75299857 None:intergenic
CTGTCGAAGAGAAAGAGAGA+AGG 0.555619 2_1:+75299827 Msa0225210:five_prime_UTR
ACAGAAAACTCGCCATGAAG+TGG 0.563345 2_1:+75299933 Msa0225210:CDS
TGTCGAAGAGAAAGAGAGAA+GGG 0.570583 2_1:+75299828 Msa0225210:five_prime_UTR
GAAGGGCAACAACCAGCCAT+GGG 0.607689 2_1:+75299845 Msa0225210:exon
GCTTAGGTACAATACAATCG+TGG 0.609230 2_1:+75299704 None:intergenic
GGAGCAGAAGACTCAACAGC+AGG 0.615251 2_1:-75300546 None:intergenic
CTTAGGTACAATACAATCGT+GGG 0.615629 2_1:+75299705 None:intergenic
TTGAGTCTTCTGCTCCTCAG+TGG 0.624600 2_1:+75300553 Msa0225210:CDS
CTTGTAGTAGTCAACACCCA+TGG 0.649177 2_1:-75299861 None:intergenic
AAGGGAGATGCAGACCACTG+AGG 0.731299 2_1:-75300567 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGATGTAAGTTTTTTTTAA+AGG + chr2_1:75300023-75300042 Msa0225210:intron 15.0%
!!! TGATGTAAGTTTTTTTTAAA+GGG + chr2_1:75300024-75300043 Msa0225210:intron 15.0%
!!! CTTAAATTTTCAATTTTTAG+TGG + chr2_1:75300050-75300069 Msa0225210:intron 15.0%
!!! ATGATGTAAGTTTTTTTTAA+AGG + chr2_1:75300023-75300042 Msa0225210:intron 15.0%
!!! TGATGTAAGTTTTTTTTAAA+GGG + chr2_1:75300024-75300043 Msa0225210:intron 15.0%
!!! CTTAAATTTTCAATTTTTAG+TGG + chr2_1:75300050-75300069 Msa0225210:intron 15.0%
!!! GGAATTTGTTTTTTGTTATT+AGG + chr2_1:75300138-75300157 Msa0225210:intron 20.0%
!! AGAAAAAACTTGATGAAATT+TGG - chr2_1:75300329-75300348 None:intergenic 20.0%
!!! CAATTTCTAGAAAAAGAAAA+GGG - chr2_1:75300588-75300607 None:intergenic 20.0%
!!! ACAATTTCTAGAAAAAGAAA+AGG - chr2_1:75300589-75300608 None:intergenic 20.0%
!!! GGAATTTGTTTTTTGTTATT+AGG + chr2_1:75300138-75300157 Msa0225210:intron 20.0%
!! AGAAAAAACTTGATGAAATT+TGG - chr2_1:75300329-75300348 None:intergenic 20.0%
!!! CAATTTCTAGAAAAAGAAAA+GGG - chr2_1:75300588-75300607 None:intergenic 20.0%
!!! ACAATTTCTAGAAAAAGAAA+AGG - chr2_1:75300589-75300608 None:intergenic 20.0%
!!! GTTCAAGAAATTTTAACGTT+GGG - chr2_1:75299745-75299764 None:intergenic 25.0%
!!! AGTTCAAGAAATTTTAACGT+TGG - chr2_1:75299746-75299765 None:intergenic 25.0%
!!! CAGCTTCTTTTTTGTTATTA+GGG - chr2_1:75299973-75299992 None:intergenic 25.0%
!!! TCAGCTTCTTTTTTGTTATT+AGG - chr2_1:75299974-75299993 None:intergenic 25.0%
!!! TTCTGAGATTTGCTTAAATT+TGG - chr2_1:75299999-75300018 None:intergenic 25.0%
!!! AAAGTTTTGTTTTTTGCATC+TGG + chr2_1:75300095-75300114 Msa0225210:intron 25.0%
!!! AAGTTTTGTTTTTTGCATCT+GGG + chr2_1:75300096-75300115 Msa0225210:intron 25.0%
! AAACAAACTAAGTAGTTCAA+TGG + chr2_1:75300401-75300420 Msa0225210:intron 25.0%
! AGAAGATATTACCATTTGTT+TGG + chr2_1:75300435-75300454 Msa0225210:intron 25.0%
!! TTTTTGTGTTCTATCATTGT+TGG - chr2_1:75300613-75300632 None:intergenic 25.0%
!!! GTTCAAGAAATTTTAACGTT+GGG - chr2_1:75299745-75299764 None:intergenic 25.0%
!!! AGTTCAAGAAATTTTAACGT+TGG - chr2_1:75299746-75299765 None:intergenic 25.0%
!!! CAGCTTCTTTTTTGTTATTA+GGG - chr2_1:75299973-75299992 None:intergenic 25.0%
!!! TCAGCTTCTTTTTTGTTATT+AGG - chr2_1:75299974-75299993 None:intergenic 25.0%
!!! TTCTGAGATTTGCTTAAATT+TGG - chr2_1:75299999-75300018 None:intergenic 25.0%
!!! AAAGTTTTGTTTTTTGCATC+TGG + chr2_1:75300095-75300114 Msa0225210:intron 25.0%
!!! AAGTTTTGTTTTTTGCATCT+GGG + chr2_1:75300096-75300115 Msa0225210:intron 25.0%
! AAACAAACTAAGTAGTTCAA+TGG + chr2_1:75300401-75300420 Msa0225210:intron 25.0%
! AGAAGATATTACCATTTGTT+TGG + chr2_1:75300435-75300454 Msa0225210:intron 25.0%
!! TTTTTGTGTTCTATCATTGT+TGG - chr2_1:75300613-75300632 None:intergenic 25.0%
! TTGTTATTAGGGTTCTTATC+AGG - chr2_1:75299962-75299981 None:intergenic 30.0%
AGAGCTGTTTATGATCAATA+TGG + chr2_1:75300181-75300200 Msa0225210:intron 30.0%
GAGCTGTTTATGATCAATAT+GGG + chr2_1:75300182-75300201 Msa0225210:intron 30.0%
AGCTGTTTATGATCAATATG+GGG + chr2_1:75300183-75300202 Msa0225210:intron 30.0%
CTAATACAATTCAGCAAGTA+GGG - chr2_1:75300499-75300518 None:intergenic 30.0%
! TTGTTATTAGGGTTCTTATC+AGG - chr2_1:75299962-75299981 None:intergenic 30.0%
AGAGCTGTTTATGATCAATA+TGG + chr2_1:75300181-75300200 Msa0225210:intron 30.0%
GAGCTGTTTATGATCAATAT+GGG + chr2_1:75300182-75300201 Msa0225210:intron 30.0%
AGCTGTTTATGATCAATATG+GGG + chr2_1:75300183-75300202 Msa0225210:intron 30.0%
CTAATACAATTCAGCAAGTA+GGG - chr2_1:75300499-75300518 None:intergenic 30.0%
! CTTTCTCTTCGACAGTTTTA+CGG - chr2_1:75299822-75299841 None:intergenic 35.0%
TTTCTTCAAGTCATCGTCTT+TGG - chr2_1:75299909-75299928 None:intergenic 35.0%
TATGATCAATATGGGGAAGA+TGG + chr2_1:75300190-75300209 Msa0225210:intron 35.0%
TGTCATGAGAACCAAACAAA+TGG - chr2_1:75300449-75300468 None:intergenic 35.0%
TCAAATAATCCAGTACTCCA+TGG + chr2_1:75300470-75300489 Msa0225210:intron 35.0%
GCTAATACAATTCAGCAAGT+AGG - chr2_1:75300500-75300519 None:intergenic 35.0%
! CTTTCTCTTCGACAGTTTTA+CGG - chr2_1:75299822-75299841 None:intergenic 35.0%
TTTCTTCAAGTCATCGTCTT+TGG - chr2_1:75299909-75299928 None:intergenic 35.0%
TATGATCAATATGGGGAAGA+TGG + chr2_1:75300190-75300209 Msa0225210:intron 35.0%
TGTCATGAGAACCAAACAAA+TGG - chr2_1:75300449-75300468 None:intergenic 35.0%
TCAAATAATCCAGTACTCCA+TGG + chr2_1:75300470-75300489 Msa0225210:intron 35.0%
GCTAATACAATTCAGCAAGT+AGG - chr2_1:75300500-75300519 None:intergenic 35.0%
! TTTTGCGGCGAATTTGTGAA+GGG - chr2_1:75299773-75299792 None:intergenic 40.0%
! ATTTTGCGGCGAATTTGTGA+AGG - chr2_1:75299774-75299793 None:intergenic 40.0%
! TCCGATTTTCTGCGATTTTG+CGG - chr2_1:75299788-75299807 None:intergenic 40.0%
TGTCGAAGAGAAAGAGAGAA+GGG + chr2_1:75299828-75299847 Msa0225210:five_prime_UTR 40.0%
TGACTACTACAAGCTTCTTC+AGG + chr2_1:75299871-75299890 Msa0225210:CDS 40.0%
!! ACAAGCTTCTTCAGGTTGAT+CGG + chr2_1:75299879-75299898 Msa0225210:CDS 40.0%
! TTTTGCGGCGAATTTGTGAA+GGG - chr2_1:75299773-75299792 None:intergenic 40.0%
! ATTTTGCGGCGAATTTGTGA+AGG - chr2_1:75299774-75299793 None:intergenic 40.0%
! TCCGATTTTCTGCGATTTTG+CGG - chr2_1:75299788-75299807 None:intergenic 40.0%
TGTCGAAGAGAAAGAGAGAA+GGG + chr2_1:75299828-75299847 Msa0225210:five_prime_UTR 40.0%
TGACTACTACAAGCTTCTTC+AGG + chr2_1:75299871-75299890 Msa0225210:CDS 40.0%
!! ACAAGCTTCTTCAGGTTGAT+CGG + chr2_1:75299879-75299898 Msa0225210:CDS 40.0%
GCCGCAAAATCGCAGAAAAT+CGG + chr2_1:75299784-75299803 Msa0225210:five_prime_UTR 45.0%
! GACAGTTTTACGGCACTTTC+TGG - chr2_1:75299812-75299831 None:intergenic 45.0%
CTGTCGAAGAGAAAGAGAGA+AGG + chr2_1:75299827-75299846 Msa0225210:five_prime_UTR 45.0%
CTTGTAGTAGTCAACACCCA+TGG - chr2_1:75299864-75299883 None:intergenic 45.0%
ACAGAAAACTCGCCATGAAG+TGG + chr2_1:75299933-75299952 Msa0225210:CDS 45.0%
! CTTATCAGGATGCCACTTCA+TGG - chr2_1:75299948-75299967 None:intergenic 45.0%
!!! TCTGGGTCATGCTGATTTTG+TGG + chr2_1:75300113-75300132 Msa0225210:intron 45.0%
!!! GGTCATGCTGATTTTGTGGT+GGG + chr2_1:75300117-75300136 Msa0225210:intron 45.0%
TCATACCTTGAGCCAACAAC+AGG + chr2_1:75300521-75300540 Msa0225210:intron 45.0%
GCCGCAAAATCGCAGAAAAT+CGG + chr2_1:75299784-75299803 Msa0225210:five_prime_UTR 45.0%
! GACAGTTTTACGGCACTTTC+TGG - chr2_1:75299812-75299831 None:intergenic 45.0%
CTGTCGAAGAGAAAGAGAGA+AGG + chr2_1:75299827-75299846 Msa0225210:five_prime_UTR 45.0%
CTTGTAGTAGTCAACACCCA+TGG - chr2_1:75299864-75299883 None:intergenic 45.0%
ACAGAAAACTCGCCATGAAG+TGG + chr2_1:75299933-75299952 Msa0225210:CDS 45.0%
! CTTATCAGGATGCCACTTCA+TGG - chr2_1:75299948-75299967 None:intergenic 45.0%
!!! TCTGGGTCATGCTGATTTTG+TGG + chr2_1:75300113-75300132 Msa0225210:intron 45.0%
!!! GGTCATGCTGATTTTGTGGT+GGG + chr2_1:75300117-75300136 Msa0225210:intron 45.0%
TCATACCTTGAGCCAACAAC+AGG + chr2_1:75300521-75300540 Msa0225210:intron 45.0%
TAGTAGTCAACACCCATGGC+TGG - chr2_1:75299860-75299879 None:intergenic 50.0%
!!! GGGTCATGCTGATTTTGTGG+TGG + chr2_1:75300116-75300135 Msa0225210:intron 50.0%
TTCAGCAAGTAGGGAGTCCA+TGG - chr2_1:75300490-75300509 None:intergenic 50.0%
!! AGGAACCTGTTGTTGGCTCA+AGG - chr2_1:75300529-75300548 None:intergenic 50.0%
CAACAGCAGGAACCTGTTGT+TGG - chr2_1:75300536-75300555 None:intergenic 50.0%
TTGAGTCTTCTGCTCCTCAG+TGG + chr2_1:75300553-75300572 Msa0225210:CDS 50.0%
TAGTAGTCAACACCCATGGC+TGG - chr2_1:75299860-75299879 None:intergenic 50.0%
!!! GGGTCATGCTGATTTTGTGG+TGG + chr2_1:75300116-75300135 Msa0225210:intron 50.0%
TTCAGCAAGTAGGGAGTCCA+TGG - chr2_1:75300490-75300509 None:intergenic 50.0%
!! AGGAACCTGTTGTTGGCTCA+AGG - chr2_1:75300529-75300548 None:intergenic 50.0%
CAACAGCAGGAACCTGTTGT+TGG - chr2_1:75300536-75300555 None:intergenic 50.0%
TTGAGTCTTCTGCTCCTCAG+TGG + chr2_1:75300553-75300572 Msa0225210:CDS 50.0%
AGAAGGGCAACAACCAGCCA+TGG + chr2_1:75299844-75299863 Msa0225210:exon 55.0%
GAAGGGCAACAACCAGCCAT+GGG + chr2_1:75299845-75299864 Msa0225210:exon 55.0%
GTAGGGAGTCCATGGAGTAC+TGG - chr2_1:75300482-75300501 None:intergenic 55.0%
GGAGCAGAAGACTCAACAGC+AGG - chr2_1:75300549-75300568 None:intergenic 55.0%
AAGGGAGATGCAGACCACTG+AGG - chr2_1:75300570-75300589 None:intergenic 55.0%
AGAAGGGCAACAACCAGCCA+TGG + chr2_1:75299844-75299863 Msa0225210:exon 55.0%
GAAGGGCAACAACCAGCCAT+GGG + chr2_1:75299845-75299864 Msa0225210:exon 55.0%
GTAGGGAGTCCATGGAGTAC+TGG - chr2_1:75300482-75300501 None:intergenic 55.0%
GGAGCAGAAGACTCAACAGC+AGG - chr2_1:75300549-75300568 None:intergenic 55.0%
AAGGGAGATGCAGACCACTG+AGG - chr2_1:75300570-75300589 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 75299707 75300674 75299707 ID=Msa0225210;Name=Msa0225210
chr2_1 mRNA 75299707 75300674 75299707 ID=Msa0225210-mRNA-1;Parent=Msa0225210;Name=Msa0225210-mRNA-1;_AED=0.21;_eAED=0.21;_QI=156|0|0.5|0.5|1|1|2|0|98
chr2_1 exon 75299707 75300027 75299707 ID=Msa0225210-mRNA-1:exon:22505;Parent=Msa0225210-mRNA-1
chr2_1 exon 75300543 75300674 75300543 ID=Msa0225210-mRNA-1:exon:22506;Parent=Msa0225210-mRNA-1
chr2_1 five_prime_UTR 75299707 75299862 75299707 ID=Msa0225210-mRNA-1:five_prime_utr;Parent=Msa0225210-mRNA-1
chr2_1 CDS 75299863 75300027 75299863 ID=Msa0225210-mRNA-1:cds;Parent=Msa0225210-mRNA-1
chr2_1 CDS 75300543 75300674 75300543 ID=Msa0225210-mRNA-1:cds;Parent=Msa0225210-mRNA-1
Gene Sequence

>Msa0225210

ATGGGTGTTGACTACTACAAGCTTCTTCAGGTTGATCGGAACGCCAAAGACGATGACTTGAAGAAAGCTTACAGAAAACTCGCCATGAAGTGGCATCCTGATAAGAACCCTAATAACAAAAAAGAAGCTGAAGCCAAATTTAAGCAAATCTCAGAAGCTTATGATGTTCCTGCTGTTGAGTCTTCTGCTCCTCAGTGGTCTGCATCTCCCTTTTCTTTTTCTAGAAATTGTTCCAACAATGATAGAACACAAAAACTAAACAAAGAGCAGAATGAGCTTGTTGTTCAAGTAAGTTAA

Protein sequence

>Msa0225210

MGVDYYKLLQVDRNAKDDDLKKAYRKLAMKWHPDKNPNNKKEAEAKFKQISEAYDVPAVESSAPQWSASPFSFSRNCSNNDRTQKLNKEQNELVVQVS*