AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0686080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0686080 MtrunA17_Chr4g0034871 95.631 206 0 1 1 197 1 206 6.87e-145 401
Msa0686080 MtrunA17_Chr4g0004661 84.058 207 23 2 1 197 57 263 3.83e-127 358
Msa0686080 MtrunA17_Chr2g0331691 81.463 205 28 2 3 197 5 209 2.44e-122 344
Msa0686080 MtrunA17_Chr6g0466301 69.082 207 53 3 1 197 1 206 1.04e-100 289
Msa0686080 MtrunA17_Chr6g0465491 69.082 207 53 3 1 197 1 206 1.04e-100 289
Msa0686080 MtrunA17_Chr1g0201121 67.464 209 54 3 1 197 1 207 2.00e-97 281
Msa0686080 MtrunA17_Chr8g0383261 61.576 203 68 2 4 197 8 209 1.64e-89 261
Msa0686080 MtrunA17_Chr6g0466161 55.307 179 79 1 19 197 10 187 1.12e-66 203
Msa0686080 MtrunA17_Chr6g0465471 55.307 179 79 1 19 197 10 187 2.39e-66 202
Msa0686080 MtrunA17_Chr6g0466431 67.327 101 33 0 1 101 1 101 7.82e-48 152
Msa0686080 MtrunA17_Chr6g0465611 67.327 101 33 0 1 101 1 101 7.82e-48 152
Msa0686080 MtrunA17_Chr4g0032201 37.019 208 104 6 8 195 12 212 4.78e-37 128
Msa0686080 MtrunA17_Chr1g0182321 37.931 203 103 8 2 190 5 198 1.30e-35 124
Msa0686080 MtrunA17_Chr2g0276941 36.323 223 102 8 1 190 1 216 1.54e-35 124
Msa0686080 MtrunA17_Chr1g0182301 37.438 203 104 8 2 190 5 198 4.57e-35 123
Msa0686080 MtrunA17_Chr2g0276931 38.690 168 87 4 8 166 9 169 1.94e-34 120
Msa0686080 MtrunA17_Chr7g0263071 38.342 193 100 7 8 190 15 198 2.72e-34 120
Msa0686080 MtrunA17_Chr1g0212521 38.144 194 95 7 8 192 15 192 4.49e-34 120
Msa0686080 MtrunA17_Chr4g0030911 37.563 197 104 7 8 194 15 202 5.66e-34 120
Msa0686080 MtrunA17_Chr5g0400131 36.364 209 100 7 10 197 11 207 6.31e-34 119
Msa0686080 MtrunA17_Chr2g0283131 35.714 210 105 8 8 195 13 214 1.46e-33 119
Msa0686080 MtrunA17_Chr2g0327911 39.286 168 88 5 8 166 15 177 2.48e-33 118
Msa0686080 MtrunA17_Chr4g0055081 35.577 208 102 7 10 197 11 206 1.26e-32 116
Msa0686080 MtrunA17_Chr7g0250531 35.500 200 109 6 8 195 8 199 2.21e-32 115
Msa0686080 MtrunA17_Chr3g0112681 38.095 168 88 4 8 166 12 172 2.30e-32 116
Msa0686080 MtrunA17_Chr4g0055651 38.095 168 88 4 8 166 8 168 2.57e-32 115
Msa0686080 MtrunA17_Chr8g0335471 35.000 200 111 5 8 195 8 200 3.35e-32 115
Msa0686080 MtrunA17_Chr4g0046261 34.286 210 108 8 8 195 13 214 3.61e-32 115
Msa0686080 MtrunA17_Chr8g0382221 36.585 205 105 7 10 197 11 207 2.07e-31 113
Msa0686080 MtrunA17_Chr3g0123971 35.784 204 107 5 10 197 11 206 5.52e-31 112
Msa0686080 MtrunA17_Chr4g0040781 33.333 210 110 8 8 195 13 214 8.08e-31 112
Msa0686080 MtrunA17_Chr1g0174281 35.829 187 104 4 8 185 15 194 9.72e-30 109
Msa0686080 MtrunA17_Chr6g0458081 36.628 172 93 4 8 170 6 170 1.43e-29 108
Msa0686080 MtrunA17_Chr8g0390561 36.471 170 92 4 10 170 11 173 3.21e-29 107
Msa0686080 MtrunA17_Chr3g0123821 34.184 196 111 5 8 194 16 202 3.97e-29 107
Msa0686080 MtrunA17_Chr8g0351861 38.272 162 84 5 8 160 16 170 1.02e-28 107
Msa0686080 MtrunA17_Chr2g0315551 32.536 209 113 8 8 195 13 214 1.15e-28 106
Msa0686080 MtrunA17_Chr3g0109721 36.364 165 89 4 10 165 12 169 1.98e-28 105
Msa0686080 MtrunA17_Chr7g0214241 33.929 168 95 4 8 166 15 175 3.48e-28 105
Msa0686080 MtrunA17_Chr2g0325091 33.014 209 111 8 8 195 11 211 4.54e-28 105
Msa0686080 MtrunA17_Chr2g0290891 34.171 199 112 5 8 194 6 197 6.40e-28 104
Msa0686080 MtrunA17_Chr5g0433561 35.758 165 90 4 10 165 12 169 8.11e-28 103
Msa0686080 MtrunA17_Chr8g0381841 34.545 165 92 4 8 163 17 174 1.25e-27 103
Msa0686080 MtrunA17_Chr7g0231131 37.576 165 87 4 8 163 15 172 1.38e-27 103
Msa0686080 MtrunA17_Chr1g0189361 33.929 168 95 4 8 166 14 174 4.97e-27 102
Msa0686080 MtrunA17_Chr2g0319951 34.545 165 92 3 8 163 13 170 2.16e-26 100
Msa0686080 MtrunA17_Chr7g0234201 32.738 168 97 4 8 166 13 173 3.36e-26 100
Msa0686080 MtrunA17_Chr8g0346421 33.032 221 117 9 1 197 19 232 3.76e-26 100
Msa0686080 MtrunA17_Chr6g0482721 35.185 162 89 4 8 160 12 166 4.48e-26 99.8
Msa0686080 MtrunA17_Chr8g0349711 32.571 175 102 4 5 170 3 170 1.85e-25 98.2
Msa0686080 MtrunA17_Chr8g0349681 35.673 171 94 4 9 170 176 339 2.06e-25 102
Msa0686080 MtrunA17_Chr8g0349681 33.981 206 113 7 8 197 384 582 8.04e-24 98.2
Msa0686080 MtrunA17_Chr8g0349681 33.136 169 97 4 5 164 10 171 5.51e-22 92.8
Msa0686080 MtrunA17_Chr7g0275991 36.420 162 87 4 8 160 20 174 4.20e-25 97.4
Msa0686080 MtrunA17_Chr6g0465601 57.143 70 30 0 99 168 3 72 4.52e-25 93.2
Msa0686080 MtrunA17_Chr1g0199331 34.132 167 93 5 9 165 32 191 8.74e-25 95.9
Msa0686080 MtrunA17_Chr8g0392931 35.185 162 89 4 8 160 14 168 9.76e-25 96.3
Msa0686080 MtrunA17_Chr6g0466421 59.091 66 27 0 103 168 17 82 4.38e-24 90.9
Msa0686080 MtrunA17_Chr8g0384311 33.140 172 100 5 8 170 13 178 6.06e-24 94.0
Msa0686080 MtrunA17_Chr1g0159481 33.721 172 99 5 8 170 17 182 5.98e-23 91.7
Msa0686080 MtrunA17_Chr7g0265001 34.545 165 92 6 10 164 18 176 1.05e-21 88.2
Msa0686080 MtrunA17_Chr1g0197331 32.990 194 110 6 9 191 16 200 2.07e-21 87.4
Msa0686080 MtrunA17_Chr1g0180291 30.208 192 111 6 9 191 15 192 1.20e-20 85.5
Msa0686080 MtrunA17_Chr3g0130271 31.285 179 107 6 2 170 6 178 1.36e-20 85.1
Msa0686080 MtrunA17_Chr2g0326141 64.912 57 20 0 120 176 299 355 1.28e-17 79.3
Msa0686080 MtrunA17_Chr3g0138121 28.313 166 100 4 9 165 14 169 5.85e-17 75.9
Msa0686080 MtrunA17_Chr3g0138101 29.480 173 89 7 9 165 14 169 5.85e-17 75.9
Msa0686080 MtrunA17_Chr3g0138111 29.480 173 89 7 9 165 14 169 6.49e-17 75.9
Msa0686080 MtrunA17_Chr6g0486041 27.670 206 117 6 8 195 6 197 7.85e-15 70.1
Msa0686080 MtrunA17_Chr2g0325101 26.816 179 107 5 8 168 6 178 2.62e-11 60.1
Msa0686080 MtrunA17_Chr3g0117931 26.425 193 118 5 1 175 1 187 6.28e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0686080 AT3G18820.1 88.835 206 14 1 1 197 1 206 1.41e-135 378
Msa0686080 AT1G49300.2 84.951 206 22 1 1 197 1 206 1.99e-129 362
Msa0686080 AT1G49300.1 84.951 206 22 1 1 197 1 206 1.99e-129 362
Msa0686080 AT1G52280.1 79.612 206 33 1 1 197 1 206 1.54e-120 340
Msa0686080 AT3G16100.1 78.155 206 36 1 1 197 1 206 3.82e-120 339
Msa0686080 AT4G09720.1 66.505 206 60 1 1 197 1 206 3.78e-101 291
Msa0686080 AT4G09720.4 64.929 211 60 2 1 197 1 211 8.50e-98 282
Msa0686080 AT4G09720.3 63.134 217 60 2 1 197 1 217 1.63e-97 282
Msa0686080 AT1G22740.1 66.667 207 55 3 1 197 1 203 4.02e-96 278
Msa0686080 AT2G21880.1 60.476 210 65 3 4 197 5 212 1.10e-87 257
Msa0686080 AT4G09720.6 67.836 171 55 0 27 197 2 172 7.81e-87 253
Msa0686080 AT4G09720.5 67.836 171 55 0 27 197 2 172 7.81e-87 253
Msa0686080 AT4G09720.2 67.836 171 55 0 27 197 2 172 7.81e-87 253
Msa0686080 AT2G21880.4 61.828 186 62 2 19 197 4 187 6.77e-81 239
Msa0686080 AT2G21880.3 61.828 186 62 2 19 197 4 187 6.77e-81 239
Msa0686080 AT2G21880.2 56.667 210 65 4 4 197 5 204 4.93e-78 232
Msa0686080 AT5G39620.1 55.233 172 68 1 6 168 3 174 1.01e-64 198
Msa0686080 AT5G59840.1 37.563 197 107 6 8 194 15 205 7.17e-36 125
Msa0686080 AT3G53610.2 38.021 192 103 6 8 189 15 200 4.97e-35 123
Msa0686080 AT3G53610.3 38.021 192 103 6 8 189 15 200 4.97e-35 123
Msa0686080 AT3G53610.1 38.021 192 103 6 8 189 15 200 4.97e-35 123
Msa0686080 AT3G46060.2 36.548 197 109 6 8 194 15 205 1.64e-34 122
Msa0686080 AT3G46060.3 36.548 197 109 6 8 194 15 205 1.64e-34 122
Msa0686080 AT3G46060.1 36.548 197 109 6 8 194 15 205 1.64e-34 122
Msa0686080 AT1G09630.1 38.596 171 89 4 8 169 12 175 3.81e-34 120
Msa0686080 AT1G28550.1 35.533 197 101 6 8 185 13 202 7.07e-34 120
Msa0686080 AT2G33870.1 34.286 210 109 7 8 195 13 215 7.70e-34 120
Msa0686080 AT3G11730.1 38.764 178 93 4 1 169 1 171 4.33e-33 117
Msa0686080 AT2G44610.1 36.190 210 100 7 10 197 11 208 7.28e-33 117
Msa0686080 AT4G18430.1 33.333 210 110 8 8 195 13 214 1.26e-32 117
Msa0686080 AT4G17530.1 35.176 199 111 5 8 195 8 199 1.73e-32 116
Msa0686080 AT5G47200.1 35.176 199 111 5 8 195 8 199 3.40e-32 115
Msa0686080 AT5G60860.1 34.762 210 107 8 8 195 13 214 5.10e-32 115
Msa0686080 AT1G07410.1 34.135 208 109 7 8 195 12 211 5.38e-32 115
Msa0686080 AT5G03520.1 41.341 179 84 8 2 166 5 176 7.63e-32 115
Msa0686080 AT3G15060.1 34.286 210 108 8 8 195 13 214 7.66e-32 115
Msa0686080 AT3G46830.1 38.182 165 86 4 8 163 12 169 9.29e-32 114
Msa0686080 AT3G46830.2 38.182 165 86 4 8 163 12 169 9.29e-32 114
Msa0686080 AT5G45750.1 34.450 209 108 9 8 195 13 213 9.77e-32 114
Msa0686080 AT5G59150.1 36.970 165 88 4 8 163 12 169 1.23e-31 114
Msa0686080 AT3G12160.1 36.842 190 104 4 8 188 15 197 2.27e-31 114
Msa0686080 AT3G09900.1 41.341 179 84 8 2 166 5 176 3.92e-31 113
Msa0686080 AT5G64990.1 36.842 209 100 7 10 197 9 206 8.04e-31 112
Msa0686080 AT1G02130.1 34.000 200 113 6 8 195 8 200 1.96e-30 111
Msa0686080 AT4G35860.1 36.842 171 92 4 8 169 6 169 7.44e-30 109
Msa0686080 AT4G18800.1 34.783 184 94 5 8 173 13 188 1.90e-29 108
Msa0686080 AT1G05810.1 31.776 214 119 6 4 197 8 214 4.34e-29 108
Msa0686080 AT5G47960.1 33.019 212 111 6 8 195 15 219 5.55e-29 107
Msa0686080 AT5G10260.1 34.615 208 105 7 10 197 11 207 1.20e-28 106
Msa0686080 AT1G05810.2 31.776 214 119 6 4 197 54 260 1.31e-28 107
Msa0686080 AT1G16920.1 32.212 208 114 8 8 195 13 213 2.03e-28 106
Msa0686080 AT4G39990.1 32.990 194 109 5 8 192 17 198 2.30e-28 106
Msa0686080 AT5G03520.2 44.697 132 67 4 35 166 41 166 2.84e-28 105
Msa0686080 AT5G65270.1 34.054 185 98 5 8 175 17 194 3.01e-28 105
Msa0686080 AT5G64990.2 35.648 216 100 8 10 197 9 213 4.16e-28 105
Msa0686080 AT2G31680.1 33.333 168 96 4 8 166 12 172 4.50e-28 105
Msa0686080 AT4G17170.1 34.171 199 112 5 8 194 6 197 5.24e-28 105
Msa0686080 AT4G17160.1 36.471 170 92 4 9 169 7 169 5.73e-28 104
Msa0686080 AT1G06400.1 35.152 165 91 4 8 163 13 170 7.07e-28 104
Msa0686080 AT1G73640.1 37.037 162 86 4 8 160 13 167 2.08e-27 103
Msa0686080 AT4G19640.1 33.880 183 102 5 10 180 12 187 4.74e-27 102
Msa0686080 AT5G45130.1 35.152 165 91 4 10 165 12 169 7.58e-27 101
Msa0686080 AT1G18200.1 36.970 165 88 5 8 163 13 170 8.53e-27 102
Msa0686080 AT2G43130.1 33.333 168 96 4 8 166 12 172 1.58e-26 101
Msa0686080 AT4G39890.1 35.152 165 92 3 9 164 10 168 2.11e-26 100
Msa0686080 AT1G01200.1 34.320 169 96 3 1 160 21 183 2.72e-26 101
Msa0686080 AT2G22290.1 32.843 204 112 5 10 197 11 205 6.85e-26 99.4
Msa0686080 AT5G47520.1 33.333 168 96 4 8 166 14 174 9.23e-26 99.4
Msa0686080 AT5G45130.2 34.969 163 90 4 12 165 22 177 1.18e-25 99.0
Msa0686080 AT3G07410.1 32.143 168 98 4 8 166 12 172 2.86e-25 98.2
Msa0686080 AT3G54840.1 32.955 176 100 6 1 165 25 193 3.29e-24 95.1
Msa0686080 AT5G59150.2 39.024 123 68 3 41 163 3 118 7.48e-24 93.2
Msa0686080 AT3G54840.3 33.333 165 93 5 11 165 2 159 1.42e-23 92.4
Msa0686080 AT3G54840.2 32.558 172 98 6 1 161 25 189 4.72e-23 91.7
Msa0686080 AT4G35860.2 40.476 126 67 4 41 165 2 120 5.04e-23 90.9
Msa0686080 AT1G43890.3 34.302 172 98 6 8 170 13 178 6.33e-23 91.7
Msa0686080 AT1G43890.1 34.302 172 98 6 8 170 13 178 6.33e-23 91.7
Msa0686080 AT1G43890.2 34.302 172 98 6 8 170 13 178 6.33e-23 91.7
Msa0686080 AT4G19640.2 34.307 137 76 3 10 137 12 143 1.59e-21 86.7
Msa0686080 AT5G03530.2 32.461 191 106 7 10 191 15 191 3.11e-21 87.4
Msa0686080 AT5G03530.1 32.461 191 106 7 10 191 15 191 3.11e-21 87.4
Msa0686080 AT4G19640.3 35.833 120 64 2 10 120 12 127 2.59e-20 84.3
Msa0686080 AT3G09910.3 30.052 193 110 7 9 191 14 191 1.53e-18 80.1
Msa0686080 AT3G09910.1 30.052 193 110 7 9 191 14 191 1.53e-18 80.1
Msa0686080 AT3G09910.2 30.052 193 110 7 9 191 14 191 1.53e-18 80.1
Msa0686080 AT3G09910.5 31.515 165 98 5 9 164 14 172 5.51e-18 78.6
Msa0686080 AT3G09910.4 31.515 165 98 5 9 164 14 172 5.51e-18 78.6
Msa0686080 AT5G55190.1 29.480 173 89 7 9 165 14 169 7.51e-17 75.9
Msa0686080 AT5G20010.1 29.480 173 89 7 9 165 14 169 9.14e-17 75.9
Msa0686080 AT5G20020.1 29.480 173 89 7 9 165 14 169 9.53e-17 75.9
Msa0686080 AT5G55080.1 26.455 189 116 4 9 184 14 192 1.24e-15 72.8
Msa0686080 AT4G28950.1 29.189 185 109 6 8 176 6 184 2.46e-15 71.6
Msa0686080 AT5G62880.1 27.807 187 111 5 1 169 1 181 5.89e-12 62.8
Msa0686080 AT5G45970.1 26.667 180 108 5 8 169 6 179 8.40e-12 62.0
Msa0686080 AT3G48040.1 27.807 187 111 5 1 169 1 181 5.70e-11 59.7

Find 120 sgRNAs with CRISPR-Local

Find 285 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGCAGATGTCTCAAAATA+TGG 0.180049 4_4:+50401998 None:intergenic
TTATCGACTACTGATGTATT+TGG 0.193524 4_4:-50405001 Msa0686080:five_prime_UTR
CAAGAATCTTCCACGGAATT+TGG 0.204645 4_4:-50405226 Msa0686080:five_prime_UTR
TGGAAACCCCTCAACAAATA+AGG 0.255068 4_4:+50405674 None:intergenic
ATCTTCAAATTGCACTTCTT+TGG 0.266812 4_4:+50403678 None:intergenic
ATGTAATTTAATTATGAATA+TGG 0.274110 4_4:+50405378 None:intergenic
TTCGACATTAATACCTTCTT+TGG 0.296445 4_4:+50401979 None:intergenic
TATCACTCTCTCTTATAAAT+AGG 0.308272 4_4:+50405783 None:intergenic
TCTCTCTATCGTAAATCTTA+AGG 0.319379 4_4:+50404954 None:intergenic
GGGCATCCTTTGCTATGGTT+TGG 0.331267 4_4:+50401948 None:intergenic
AAGAAGTGCAATTTGAAGAT+AGG 0.335741 4_4:-50403675 Msa0686080:CDS
CGATTTGTTGGGTGCTTGTT+TGG 0.339053 4_4:-50401224 Msa0686080:three_prime_UTR
TTGTAGTGATAATGAAAATA+TGG 0.348003 4_4:-50405503 Msa0686080:five_prime_UTR
CACTATGTGGAAGAAATTAC+AGG 0.355406 4_4:+50405730 None:intergenic
TTTCTCCCCTTATTTGTTGA+GGG 0.378549 4_4:-50405681 Msa0686080:five_prime_UTR
TGATAATGAAAATATGGTTT+AGG 0.388362 4_4:-50405497 Msa0686080:five_prime_UTR
CAAGTTTAGTGTTTAGATGA+AGG 0.389268 4_4:+50405304 None:intergenic
ATTCCTCCCTCCAGTTGTTA+AGG 0.391109 4_4:+50402446 None:intergenic
CTGGTACCTAACACCATATA+TGG 0.393170 4_4:-50405578 Msa0686080:five_prime_UTR
AAACCACTCGTTAAATCTAA+TGG 0.396648 4_4:-50405119 Msa0686080:five_prime_UTR
CTCTTGAAAGTCATCATTCT+CGG 0.403803 4_4:-50404774 Msa0686080:CDS
GGAAACCCCTCAACAAATAA+GGG 0.407921 4_4:+50405675 None:intergenic
CATTTGACAACCTTAACAAC+TGG 0.410397 4_4:-50402456 Msa0686080:CDS
AAGGGCAGAAGAACCATATA+TGG 0.414404 4_4:+50405565 None:intergenic
AGAAAGCAACTCCTAGGCTT+TGG 0.417205 4_4:+50402530 None:intergenic
GAAGAACCATATATGGTGTT+AGG 0.417878 4_4:+50405572 None:intergenic
AGCAATCAGCCAAGGTCATC+AGG 0.423555 4_4:-50401390 Msa0686080:CDS
TTTGGTGGGTTGGAGAGGAC+AGG 0.423576 4_4:-50405208 Msa0686080:five_prime_UTR
ACACACAAATAAAAGACAAA+AGG 0.429142 4_4:+50405546 None:intergenic
CTTTCAAGAGCGTTCTTCTA+CGG 0.436158 4_4:+50404787 None:intergenic
TAGCAGCACGGATCGATTAG+AGG 0.440685 4_4:+50405456 None:intergenic
TTCCACGGAATTTGGTGGGT+TGG 0.448127 4_4:-50405218 Msa0686080:five_prime_UTR
GCAAAGGATGCCCTGAAAAG+TGG 0.450910 4_4:-50401938 Msa0686080:CDS
TTGTTTCCTATAACAACAAA+AGG 0.451159 4_4:+50402236 None:intergenic
ACAACAAAAGGAAAATTCTC+TGG 0.451708 4_4:+50402248 None:intergenic
GGAGGAAGAGTTAACCGCTT+TGG 0.458775 4_4:+50404828 None:intergenic
TTTCCAACATCAATTGTGTC+TGG 0.462911 4_4:+50401414 None:intergenic
TGCACAGGTTTCAGAAAAGA+AGG 0.467310 4_4:-50402053 Msa0686080:intron
TGTGTATATACATACTTCTC+TGG 0.469606 4_4:-50405526 Msa0686080:five_prime_UTR
TTTCAGGGCATCCTTTGCTA+TGG 0.470505 4_4:+50401943 None:intergenic
CTAGGCTTTGGAATCTCTCC+TGG 0.472910 4_4:+50402542 None:intergenic
TAGGAGTTGCTTTCTATCGT+GGG 0.475937 4_4:-50402522 Msa0686080:CDS
TGGCAGATGTCTCAAAATAT+GGG 0.483704 4_4:+50401999 None:intergenic
TTTGAGACATCTGCCAAAGA+AGG 0.487428 4_4:-50401992 Msa0686080:CDS
TTTCAAGAGCGTTCTTCTAC+GGG 0.490904 4_4:+50404788 None:intergenic
CACACAAATAAAAGACAAAA+GGG 0.494918 4_4:+50405547 None:intergenic
TCTTCTGTGAGGTTTCTCCT+AGG 0.495248 4_4:-50405825 Msa0686080:five_prime_UTR
GGAAAATTCTCTGGATCGGA+AGG 0.495526 4_4:+50402257 None:intergenic
CGGGCATGGTGTGCAGCAAA+AGG 0.496942 4_4:-50402028 Msa0686080:CDS
CAACATCAATTGTGTCTGGC+AGG 0.497094 4_4:+50401418 None:intergenic
GTCTAGCCGTTGCTGTCTTG+AGG 0.499682 4_4:-50401293 Msa0686080:three_prime_UTR
CAATGTTGGAATAGCATACA+AGG 0.501772 4_4:+50401171 None:intergenic
AAATAAGGGGAGAAAACCAC+GGG 0.502994 4_4:+50405689 None:intergenic
CGGAATTTGGTGGGTTGGAG+AGG 0.504576 4_4:-50405213 Msa0686080:five_prime_UTR
TGCCCTGAAAAGTGGGGAAG+AGG 0.506394 4_4:-50401930 Msa0686080:CDS
GGAAACAAGATCGACATTGA+TGG 0.507185 4_4:-50402221 Msa0686080:CDS
GATTTGGGATACAGCTGGCC+AGG 0.507917 4_4:-50402560 Msa0686080:CDS
GGTTAAGAAGTCAGCTCCAA+TGG 0.508254 4_4:+50403699 None:intergenic
CTAGGAGTTGCTTTCTATCG+TGG 0.516699 4_4:-50402523 Msa0686080:CDS
AATCAGTACAAGGCAACCAT+TGG 0.525698 4_4:-50403715 Msa0686080:CDS
TTCTCCCCTTATTTGTTGAG+GGG 0.525716 4_4:-50405680 Msa0686080:five_prime_UTR
TTTACGTGCTCCACCGGTGA+GGG 0.525719 4_4:-50405091 Msa0686080:five_prime_UTR
GCACCATTCACAAACCTGTA+AGG 0.531833 4_4:+50403645 None:intergenic
TTGGTGGGTTGGAGAGGACA+GGG 0.533069 4_4:-50405207 Msa0686080:five_prime_UTR
TTGCAGATTTGGGATACAGC+TGG 0.533863 4_4:-50402565 Msa0686080:intron
AAAATAAGAGACGAGAAAAG+AGG 0.534192 4_4:+50405628 None:intergenic
CAAAGGATGCCCTGAAAAGT+GGG 0.534379 4_4:-50401937 Msa0686080:CDS
GGACAGGGAAGATATAATGC+AGG 0.538844 4_4:-50405192 Msa0686080:five_prime_UTR
GAATCTTCCACGGAATTTGG+TGG 0.539434 4_4:-50405223 Msa0686080:five_prime_UTR
AGGTTTCAGAAAAGAAGGCT+CGG 0.540140 4_4:-50402048 Msa0686080:CDS
TAATTAAATTACATCATCCA+TGG 0.542308 4_4:-50405369 Msa0686080:five_prime_UTR
TCATCATTCTCGGTGACAGC+GGG 0.542655 4_4:-50404764 Msa0686080:intron
AAAAGGAAAATTCTCTGGAT+CGG 0.543661 4_4:+50402253 None:intergenic
AAAATAATATATCTTTCATG+TGG 0.544640 4_4:+50405272 None:intergenic
AATCTTCCACGGAATTTGGT+GGG 0.544838 4_4:-50405222 Msa0686080:five_prime_UTR
CTCTCCTTGTATTTAGAGAG+AGG 0.549711 4_4:-50405754 Msa0686080:five_prime_UTR
CTGCCAGACACAATTGATGT+TGG 0.557538 4_4:-50401417 Msa0686080:CDS
GGTTTCAGAAAAGAAGGCTC+GGG 0.559631 4_4:-50402047 Msa0686080:CDS
CACTCACATCCTGATGACCT+TGG 0.564551 4_4:+50401381 None:intergenic
CACGATAGAAAGCAACTCCT+AGG 0.569261 4_4:+50402524 None:intergenic
TTGACAACCTTAACAACTGG+AGG 0.575421 4_4:-50402453 Msa0686080:CDS
CAGGAGAGATTCCAAAGCCT+AGG 0.576665 4_4:-50402541 Msa0686080:CDS
GCGCCATTAGATTTAACGAG+TGG 0.577744 4_4:+50405116 None:intergenic
AACAAGATCGACATTGATGG+TGG 0.578098 4_4:-50402218 Msa0686080:CDS
TTTGGATTACAGCTGTCAGT+AGG 0.586658 4_4:-50404983 Msa0686080:five_prime_UTR
GGATCAATCAAGTCCCTCAC+CGG 0.589882 4_4:+50405078 None:intergenic
AAGAGCGTTCTTCTACGGGA+AGG 0.590281 4_4:+50404792 None:intergenic
AGTAGAGAACTTTCACTATG+TGG 0.592080 4_4:+50405717 None:intergenic
AGGACCTCTCTCTAAATACA+AGG 0.594202 4_4:+50405750 None:intergenic
AAACGGTGGATTCTAGGGTG+AGG 0.594599 4_4:+50405056 None:intergenic
AACGGTGGATTCTAGGGTGA+GGG 0.594993 4_4:+50405057 None:intergenic
AGCATTCCAAACCATAGCAA+AGG 0.595720 4_4:-50401954 Msa0686080:CDS
CTCCAACCCACCAAATTCCG+TGG 0.606198 4_4:+50405216 None:intergenic
GTCATCATTCTCGGTGACAG+CGG 0.607687 4_4:-50404765 Msa0686080:CDS
CAGAAAAGAAGGCTCGGGCA+TGG 0.609079 4_4:-50402042 Msa0686080:CDS
AATACAAACTAACACAACCT+AGG 0.610253 4_4:+50405808 None:intergenic
AGGAGTTGCTTTCTATCGTG+GGG 0.614079 4_4:-50402521 Msa0686080:CDS
GAAGTTTAGTAATCAGTACA+AGG 0.614358 4_4:-50403725 Msa0686080:CDS
CGGGAAGGCATCGTTCAGAT+CGG 0.615340 4_4:+50404807 None:intergenic
AAATAATATATCTTTCATGT+GGG 0.618581 4_4:+50405273 None:intergenic
TCAATCAAGTCCCTCACCGG+TGG 0.619305 4_4:+50405081 None:intergenic
AAATTACATCATCCATGGTG+TGG 0.624191 4_4:-50405364 Msa0686080:five_prime_UTR
GAGAGAAACAGAGTTGAAGA+CGG 0.630192 4_4:-50404900 Msa0686080:five_prime_UTR
TTTGCAGCAAGAATCTTCCA+CGG 0.633537 4_4:-50405233 Msa0686080:five_prime_UTR
TTGGAAACAGCAATCAGCCA+AGG 0.645424 4_4:-50401398 Msa0686080:CDS
GAAACCCCTCAACAAATAAG+GGG 0.651668 4_4:+50405676 None:intergenic
ACAAGATCGACATTGATGGT+GGG 0.651715 4_4:-50402217 Msa0686080:CDS
ATATAATGCAGGATTGTGTG+TGG 0.652142 4_4:-50405181 Msa0686080:five_prime_UTR
CTAAACACTAAACTTGAGAG+AGG 0.654468 4_4:-50405297 Msa0686080:five_prime_UTR
TGACAACCTTAACAACTGGA+GGG 0.655521 4_4:-50402452 Msa0686080:CDS
TAACAACATTAGCCACACCA+TGG 0.660574 4_4:+50405352 None:intergenic
GAAGGCATCGTTCAGATCGG+AGG 0.665371 4_4:+50404810 None:intergenic
TGATGGTGGGAACAGTAGAG+TGG 0.665695 4_4:-50402204 Msa0686080:intron
CAAATAAGGGGAGAAAACCA+CGG 0.666116 4_4:+50405688 None:intergenic
ATGAAACCTCAAGACAGCAA+CGG 0.671829 4_4:+50401287 None:intergenic
AGTTCTCTACTCTTGCCCCG+TGG 0.676863 4_4:-50405705 Msa0686080:five_prime_UTR
CAACCTTAACAACTGGAGGG+AGG 0.688480 4_4:-50402449 Msa0686080:CDS
TCACAAAACACATACCAAAG+CGG 0.704022 4_4:-50404842 Msa0686080:five_prime_UTR
AAAGGATGCCCTGAAAAGTG+GGG 0.713593 4_4:-50401936 Msa0686080:CDS
AATAAGGGGAGAAAACCACG+GGG 0.858085 4_4:+50405690 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGTAATTTAATTATGAATA+TGG + chr4_4:50401578-50401597 None:intergenic 10.0%
!!! TGTTACTTAAAAAAAAAAAA+CGG + chr4_4:50401917-50401936 None:intergenic 10.0%
!! AATATGATAATTATGATTAT+CGG + chr4_4:50402248-50402267 None:intergenic 10.0%
!! ACTTAATTTAAAAAATTGTT+TGG - chr4_4:50402718-50402737 Msa0686080:intron 10.0%
!!! TATTGAAATTTAAGTTAATA+TGG - chr4_4:50404320-50404339 Msa0686080:intron 10.0%
!!! ATATTATTTTAAAATCACTT+TGG - chr4_4:50405634-50405653 Msa0686080:five_prime_UTR 10.0%
!!! GATAATATTTTTTTTTACTC+TGG - chr4_4:50401356-50401375 Msa0686080:three_prime_UTR 15.0%
!! AAATAATATATCTTTCATGT+GGG + chr4_4:50401683-50401702 None:intergenic 15.0%
!! AAAATAATATATCTTTCATG+TGG + chr4_4:50401684-50401703 None:intergenic 15.0%
!!! TTCACATATCTAAAATATAT+AGG + chr4_4:50403205-50403224 None:intergenic 15.0%
!!! GTACAAAATAATTTTTGTAT+GGG + chr4_4:50405865-50405884 None:intergenic 15.0%
!!! TGTACAAAATAATTTTTGTA+TGG + chr4_4:50405866-50405885 None:intergenic 15.0%
!!! TTGTAGTGATAATGAAAATA+TGG - chr4_4:50401450-50401469 Msa0686080:intron 20.0%
!!! TGATAATGAAAATATGGTTT+AGG - chr4_4:50401456-50401475 Msa0686080:intron 20.0%
!! TAATTAAATTACATCATCCA+TGG - chr4_4:50401584-50401603 Msa0686080:intron 20.0%
!! TACTTAAAAAAAAAAAACGG+TGG + chr4_4:50401914-50401933 None:intergenic 20.0%
!!! TCATTTTTCTTATTCTTGTT+TGG - chr4_4:50402372-50402391 Msa0686080:intron 20.0%
!! CTATAAGTAACTTAATTTAC+AGG - chr4_4:50402411-50402430 Msa0686080:intron 20.0%
!! AAATCAACTTAACATTAACA+TGG + chr4_4:50402754-50402773 None:intergenic 20.0%
!! AAAGAAAGAAAAGAAAAAAC+CGG + chr4_4:50402803-50402822 None:intergenic 20.0%
!! AGATAAACATTCCAAAAAAA+GGG + chr4_4:50403424-50403443 None:intergenic 20.0%
!! AAGATAAACATTCCAAAAAA+AGG + chr4_4:50403425-50403444 None:intergenic 20.0%
!! ACATATAGATTGAATCAAAT+GGG - chr4_4:50404040-50404059 Msa0686080:intron 20.0%
!!! AATTTTCCTTTTGTTGTTAT+AGG - chr4_4:50404711-50404730 Msa0686080:intron 20.0%
!! CTTCATAAAAAATGCATTAA+GGG - chr4_4:50405100-50405119 Msa0686080:five_prime_UTR 20.0%
!!! TGTTTTTTTTTTCACAATGT+TGG + chr4_4:50405799-50405818 None:intergenic 20.0%
! AAAAAATAGTTTCTTCTGTG+AGG - chr4_4:50401117-50401136 Msa0686080:three_prime_UTR 25.0%
! TATCACTCTCTCTTATAAAT+AGG + chr4_4:50401173-50401192 None:intergenic 25.0%
! CACACAAATAAAAGACAAAA+GGG + chr4_4:50401409-50401428 None:intergenic 25.0%
! ACACACAAATAAAAGACAAA+AGG + chr4_4:50401410-50401429 None:intergenic 25.0%
!!! CTTCTTTGTTTTGTTTTTCA+TGG + chr4_4:50401526-50401545 None:intergenic 25.0%
!! CGTTTTACTTAATTAAGCAT+TGG + chr4_4:50401552-50401571 None:intergenic 25.0%
! GAAGAATAAAAATAAGCAGA+AGG + chr4_4:50402296-50402315 None:intergenic 25.0%
! TAGATAACAAGAATCATATC+TGG - chr4_4:50402453-50402472 Msa0686080:CDS 25.0%
!! AGTTACTTTAGATATGATTC+TGG + chr4_4:50402701-50402720 None:intergenic 25.0%
! TTTGTACTAAATAGCTATTG+AGG + chr4_4:50402987-50403006 None:intergenic 25.0%
! TACAAAATACTGTGAAATAG+CGG - chr4_4:50403001-50403020 Msa0686080:intron 25.0%
!!! TGAGGTTTTAGTATTGTATA+GGG - chr4_4:50403140-50403159 Msa0686080:intron 25.0%
!!! GGTAATGCTTTAAAAATATG+CGG - chr4_4:50403161-50403180 Msa0686080:intron 25.0%
! TCAAAATCAAATTATGCACA+AGG + chr4_4:50403336-50403355 None:intergenic 25.0%
!!! AAATTACCAAACCCTTTTTT+TGG - chr4_4:50403410-50403429 Msa0686080:intron 25.0%
! TCTTTCTGATATTACATAGA+TGG - chr4_4:50403441-50403460 Msa0686080:intron 25.0%
! GATATTACATAGATGGTTTA+TGG - chr4_4:50403448-50403467 Msa0686080:intron 25.0%
! GATTTAATTGAAGAATTCGT+AGG - chr4_4:50403669-50403688 Msa0686080:CDS 25.0%
! CAAAAAATGCAATACACTTT+AGG + chr4_4:50403831-50403850 None:intergenic 25.0%
!!! CTAAAGTGTATTGCATTTTT+TGG - chr4_4:50403829-50403848 Msa0686080:intron 25.0%
!!! TGTATTGCATTTTTTGGAAA+CGG - chr4_4:50403835-50403854 Msa0686080:intron 25.0%
! GACATATAGATTGAATCAAA+TGG - chr4_4:50404039-50404058 Msa0686080:intron 25.0%
! CATATAGATTGAATCAAATG+GGG - chr4_4:50404041-50404060 Msa0686080:intron 25.0%
!!! TGTTAGGCTGTTTAATTTTT+TGG - chr4_4:50404164-50404183 Msa0686080:intron 25.0%
! ATTCTAAATCTTGCAGATTT+GGG - chr4_4:50404378-50404397 Msa0686080:intron 25.0%
! TTGTTTCCTATAACAACAAA+AGG + chr4_4:50404720-50404739 None:intergenic 25.0%
! AAACATGTTAGATAGCAAAA+GGG + chr4_4:50404787-50404806 None:intergenic 25.0%
! GCTTCATAAAAAATGCATTA+AGG - chr4_4:50405099-50405118 Msa0686080:five_prime_UTR 25.0%
! ATAGGATTGATGTTTAATGA+TGG - chr4_4:50405225-50405244 Msa0686080:five_prime_UTR 25.0%
! ATTAAACATCAATCCTAACA+TGG + chr4_4:50405271-50405290 None:intergenic 25.0%
! TTAGGATTGATGTTTAATGA+TGG - chr4_4:50405273-50405292 Msa0686080:five_prime_UTR 25.0%
! TCAATCTTTGCATTATAACA+TGG + chr4_4:50405319-50405338 None:intergenic 25.0%
!!! TTGGTTTGTCTATATAATAG+TGG - chr4_4:50405343-50405362 Msa0686080:five_prime_UTR 25.0%
! CAGAAAGATTCATAATCTAT+GGG + chr4_4:50405420-50405439 None:intergenic 25.0%
!! ATCTTTCTGCTATTTGATAA+TGG - chr4_4:50405431-50405450 Msa0686080:five_prime_UTR 25.0%
!! TCTTTCTGCTATTTGATAAT+GGG - chr4_4:50405432-50405451 Msa0686080:five_prime_UTR 25.0%
! AAACATAACATCAGTAACAT+TGG + chr4_4:50405472-50405491 None:intergenic 25.0%
!! ATGTTACTGATGTTATGTTT+TGG - chr4_4:50405472-50405491 Msa0686080:five_prime_UTR 25.0%
!! TGTTACTGATGTTATGTTTT+GGG - chr4_4:50405473-50405492 Msa0686080:five_prime_UTR 25.0%
! TGTGAAAAAAAAAACAGCAA+AGG - chr4_4:50405803-50405822 Msa0686080:five_prime_UTR 25.0%
AATACAAACTAACACAACCT+AGG + chr4_4:50401148-50401167 None:intergenic 30.0%
! TGAACACACAATATTTTACG+TGG + chr4_4:50401302-50401321 None:intergenic 30.0%
AAAATAAGAGACGAGAAAAG+AGG + chr4_4:50401328-50401347 None:intergenic 30.0%
TGTGTATATACATACTTCTC+TGG - chr4_4:50401427-50401446 Msa0686080:intron 30.0%
CAAGTTTAGTGTTTAGATGA+AGG + chr4_4:50401652-50401671 None:intergenic 30.0%
AAACCACTCGTTAAATCTAA+TGG - chr4_4:50401834-50401853 Msa0686080:intron 30.0%
! AAAAAAAACGGTGGATTCTA+GGG + chr4_4:50401905-50401924 None:intergenic 30.0%
! AAAAAAAAACGGTGGATTCT+AGG + chr4_4:50401906-50401925 None:intergenic 30.0%
! TTATCGACTACTGATGTATT+TGG - chr4_4:50401952-50401971 Msa0686080:CDS 30.0%
TCTCTCTATCGTAAATCTTA+AGG + chr4_4:50402002-50402021 None:intergenic 30.0%
TATAGAGTAGCTTAATGCAT+AGG - chr4_4:50402646-50402665 Msa0686080:intron 30.0%
!! GAATCATTTGTTGTATCTAC+CGG - chr4_4:50402781-50402800 Msa0686080:intron 30.0%
! GTATCCTTCGATTTTTGTAT+AGG - chr4_4:50402886-50402905 Msa0686080:intron 30.0%
ACTTCCTATACAAAAATCGA+AGG + chr4_4:50402893-50402912 None:intergenic 30.0%
! ATTTTCACTGCAATTTTCTG+AGG - chr4_4:50403122-50403141 Msa0686080:intron 30.0%
!! CTGAGGTTTTAGTATTGTAT+AGG - chr4_4:50403139-50403158 Msa0686080:intron 30.0%
GAAGTTTAGTAATCAGTACA+AGG - chr4_4:50403228-50403247 Msa0686080:intron 30.0%
ATCTTCAAATTGCACTTCTT+TGG + chr4_4:50403278-50403297 None:intergenic 30.0%
! AAGAAGTGCAATTTGAAGAT+AGG - chr4_4:50403278-50403297 Msa0686080:intron 30.0%
! GGTTTGGTAATTTGTAAATC+TGG + chr4_4:50403403-50403422 None:intergenic 30.0%
AACATTCCAAAAAAAGGGTT+TGG + chr4_4:50403419-50403438 None:intergenic 30.0%
! NNAGCCTTTTCCAACTAAAT+GGG + chr4_4:50403498-50403517 None:intergenic 30.0%
! NNNAGCCTTTTCCAACTAAA+TGG + chr4_4:50403499-50403518 None:intergenic 30.0%
ATGTTCAACCTTCATTTAAG+AGG + chr4_4:50403701-50403720 None:intergenic 30.0%
ACTTAATACAGTAAACTACC+AGG + chr4_4:50403768-50403787 None:intergenic 30.0%
!!! GTATTGCATTTTTTGGAAAC+GGG - chr4_4:50403836-50403855 Msa0686080:intron 30.0%
AACAATTTCTCAAAAACGAC+AGG + chr4_4:50403899-50403918 None:intergenic 30.0%
!! TATATTTTTGACGATTGCCT+TGG - chr4_4:50403922-50403941 Msa0686080:intron 30.0%
TACATACATGTGCAAACTAA+CGG + chr4_4:50403983-50404002 None:intergenic 30.0%
TAACAATCCTTAACCGAAAA+AGG + chr4_4:50404149-50404168 None:intergenic 30.0%
!! TTTTTCGGTTAAGGATTGTT+AGG - chr4_4:50404148-50404167 Msa0686080:intron 30.0%
!!! ATTTTTTGGACAGTACACTT+TGG - chr4_4:50404178-50404197 Msa0686080:intron 30.0%
TTTCCACAAGAAACAAAACA+TGG + chr4_4:50404255-50404274 None:intergenic 30.0%
CATTCTAAATCTTGCAGATT+TGG - chr4_4:50404377-50404396 Msa0686080:intron 30.0%
AAAAGGAAAATTCTCTGGAT+CGG + chr4_4:50404703-50404722 None:intergenic 30.0%
ACAACAAAAGGAAAATTCTC+TGG + chr4_4:50404708-50404727 None:intergenic 30.0%
GAAACATGTTAGATAGCAAA+AGG + chr4_4:50404788-50404807 None:intergenic 30.0%
CTGTTTAGCAGTACATATTT+AGG + chr4_4:50404859-50404878 None:intergenic 30.0%
TTCGACATTAATACCTTCTT+TGG + chr4_4:50404977-50404996 None:intergenic 30.0%
AGTGAACTAAAACAACTAGT+AGG + chr4_4:50405176-50405195 None:intergenic 30.0%
TCTTGGAAACAAATGCATAT+AGG - chr4_4:50405207-50405226 Msa0686080:five_prime_UTR 30.0%
TTGCATTATAACATGGCAAA+TGG + chr4_4:50405312-50405331 None:intergenic 30.0%
TAATGCAAAGATTGATGTGT+TGG - chr4_4:50405324-50405343 Msa0686080:five_prime_UTR 30.0%
GCAGAAAGATTCATAATCTA+TGG + chr4_4:50405421-50405440 None:intergenic 30.0%
!!! ATTGTTTTAGTGCGATTTGT+TGG - chr4_4:50405717-50405736 Msa0686080:five_prime_UTR 30.0%
!!! TTGTTTTAGTGCGATTTGTT+GGG - chr4_4:50405718-50405737 Msa0686080:five_prime_UTR 30.0%
CACTATGTGGAAGAAATTAC+AGG + chr4_4:50401226-50401245 None:intergenic 35.0%
AGTAGAGAACTTTCACTATG+TGG + chr4_4:50401239-50401258 None:intergenic 35.0%
!! TTTTCTCCCCTTATTTGTTG+AGG - chr4_4:50401271-50401290 Msa0686080:three_prime_UTR 35.0%
! TTTCTCCCCTTATTTGTTGA+GGG - chr4_4:50401272-50401291 Msa0686080:three_prime_UTR 35.0%
GAAGAACCATATATGGTGTT+AGG + chr4_4:50401384-50401403 None:intergenic 35.0%
!!! TTTTCATGGTTTTAGCAGCA+CGG + chr4_4:50401512-50401531 None:intergenic 35.0%
AAATTACATCATCCATGGTG+TGG - chr4_4:50401589-50401608 Msa0686080:intron 35.0%
CTAAACACTAAACTTGAGAG+AGG - chr4_4:50401656-50401675 Msa0686080:intron 35.0%
ATATAATGCAGGATTGTGTG+TGG - chr4_4:50401772-50401791 Msa0686080:intron 35.0%
TCACAAAACACATACCAAAG+CGG - chr4_4:50402111-50402130 Msa0686080:intron 35.0%
CTCTTGAAAGTCATCATTCT+CGG - chr4_4:50402179-50402198 Msa0686080:intron 35.0%
TCTCGAACTCTTCAATTCTA+GGG - chr4_4:50402218-50402237 Msa0686080:CDS 35.0%
TTGACATGCAAGCTCAAAAT+TGG - chr4_4:50402338-50402357 Msa0686080:intron 35.0%
!! TAGTGTTGTTGATGATTTGC+AGG - chr4_4:50402837-50402856 Msa0686080:intron 35.0%
!! AGTGTTGTTGATGATTTGCA+GGG - chr4_4:50402838-50402857 Msa0686080:intron 35.0%
AGGAAGTCAGTATTATGTTC+AGG - chr4_4:50402906-50402925 Msa0686080:intron 35.0%
GGAAGTCAGTATTATGTTCA+GGG - chr4_4:50402907-50402926 Msa0686080:intron 35.0%
CGAATAGTACTATTGCATAG+CGG - chr4_4:50402938-50402957 Msa0686080:intron 35.0%
! GCTATAACACTTTTGCATAG+CGG - chr4_4:50403034-50403053 Msa0686080:intron 35.0%
! AATCTGGCCTTTCATTTACA+AGG + chr4_4:50403387-50403406 None:intergenic 35.0%
! NAGCCTTTTCCAACTAAATG+GGG + chr4_4:50403497-50403516 None:intergenic 35.0%
!!! CATTTTAAAGTCATGTGCCA+AGG + chr4_4:50403942-50403961 None:intergenic 35.0%
GTTTGCACATGTATGTAATG+TGG - chr4_4:50403986-50404005 Msa0686080:intron 35.0%
! CTAATACTGAAAGAGCATGA+CGG + chr4_4:50404228-50404247 None:intergenic 35.0%
!!! CATCCATGTTTTGTTTCTTG+TGG - chr4_4:50404249-50404268 Msa0686080:intron 35.0%
CATTTGACAACCTTAACAAC+TGG - chr4_4:50404497-50404516 Msa0686080:intron 35.0%
AACTTTATGATCACTGCATC+AGG - chr4_4:50404668-50404687 Msa0686080:intron 35.0%
AGGTGTTTCATGAATGTATC+TGG + chr4_4:50404839-50404858 None:intergenic 35.0%
TGGCAGATGTCTCAAAATAT+GGG + chr4_4:50404957-50404976 None:intergenic 35.0%
TTGGCAGATGTCTCAAAATA+TGG + chr4_4:50404958-50404977 None:intergenic 35.0%
GGGTATATCTGATTATGAGA+TGG - chr4_4:50405120-50405139 Msa0686080:five_prime_UTR 35.0%
GATTATGAGATGGTGTTCAT+TGG - chr4_4:50405130-50405149 Msa0686080:five_prime_UTR 35.0%
TTCACTATATCCATGTCTCT+TGG - chr4_4:50405190-50405209 Msa0686080:five_prime_UTR 35.0%
ATCAATCCTAACATGGCAAA+TGG + chr4_4:50405264-50405283 None:intergenic 35.0%
TTTCCAACATCAATTGTGTC+TGG + chr4_4:50405542-50405561 None:intergenic 35.0%
CAATGTTGGAATAGCATACA+AGG + chr4_4:50405785-50405804 None:intergenic 35.0%
CTCTCCTTGTATTTAGAGAG+AGG - chr4_4:50401199-50401218 Msa0686080:three_prime_UTR 40.0%
AGGACCTCTCTCTAAATACA+AGG + chr4_4:50401206-50401225 None:intergenic 40.0%
AAATAAGGGGAGAAAACCAC+GGG + chr4_4:50401267-50401286 None:intergenic 40.0%
CAAATAAGGGGAGAAAACCA+CGG + chr4_4:50401268-50401287 None:intergenic 40.0%
! TTCTCCCCTTATTTGTTGAG+GGG - chr4_4:50401273-50401292 Msa0686080:three_prime_UTR 40.0%
GAAACCCCTCAACAAATAAG+GGG + chr4_4:50401280-50401299 None:intergenic 40.0%
GGAAACCCCTCAACAAATAA+GGG + chr4_4:50401281-50401300 None:intergenic 40.0%
TGGAAACCCCTCAACAAATA+AGG + chr4_4:50401282-50401301 None:intergenic 40.0%
CTGGTACCTAACACCATATA+TGG - chr4_4:50401375-50401394 Msa0686080:exon 40.0%
AAGGGCAGAAGAACCATATA+TGG + chr4_4:50401391-50401410 None:intergenic 40.0%
TAACAACATTAGCCACACCA+TGG + chr4_4:50401604-50401623 None:intergenic 40.0%
TTTGCAGCAAGAATCTTCCA+CGG - chr4_4:50401720-50401739 Msa0686080:intron 40.0%
CAAGAATCTTCCACGGAATT+TGG - chr4_4:50401727-50401746 Msa0686080:intron 40.0%
AATCTTCCACGGAATTTGGT+GGG - chr4_4:50401731-50401750 Msa0686080:intron 40.0%
TTTGGATTACAGCTGTCAGT+AGG - chr4_4:50401970-50401989 Msa0686080:CDS 40.0%
GAGAGAAACAGAGTTGAAGA+CGG - chr4_4:50402053-50402072 Msa0686080:intron 40.0%
!! TTTCAAGAGCGTTCTTCTAC+GGG + chr4_4:50402168-50402187 None:intergenic 40.0%
!! CTTTCAAGAGCGTTCTTCTA+CGG + chr4_4:50402169-50402188 None:intergenic 40.0%
CTCTCGAACTCTTCAATTCT+AGG - chr4_4:50402217-50402236 Msa0686080:CDS 40.0%
ACATCTTCGGTCAAAAGTCT+CGG - chr4_4:50402611-50402630 Msa0686080:intron 40.0%
!! TTGTTGATGATTTGCAGGGT+GGG - chr4_4:50402842-50402861 Msa0686080:intron 40.0%
ATACTGTGAAATAGCGGCTA+TGG - chr4_4:50403007-50403026 Msa0686080:intron 40.0%
AATCAGTACAAGGCAACCAT+TGG - chr4_4:50403238-50403257 Msa0686080:intron 40.0%
! AGATAGGCTTTTCACCTTAC+AGG - chr4_4:50403294-50403313 Msa0686080:intron 40.0%
TCACCTTACAGGTTTGTGAA+TGG - chr4_4:50403305-50403324 Msa0686080:intron 40.0%
! TTTACAAGGCTTGGTCATGA+AGG + chr4_4:50403373-50403392 None:intergenic 40.0%
! GACCAAGCCTTGTAAATGAA+AGG - chr4_4:50403377-50403396 Msa0686080:intron 40.0%
! CTTTTCCAACTAAATGGGGT+CGG + chr4_4:50403493-50403512 None:intergenic 40.0%
GGTCACAACCTCTTAAATGA+AGG - chr4_4:50403690-50403709 Msa0686080:CDS 40.0%
!! TGCAATACACTTTAGGATGC+AGG + chr4_4:50403824-50403843 None:intergenic 40.0%
ACATGTGCAAACTAACGGTT+AGG + chr4_4:50403978-50403997 None:intergenic 40.0%
! AGTTTTTCCGTTGCACTCTT+AGG - chr4_4:50404121-50404140 Msa0686080:intron 40.0%
!!! TTAGGAGCCTTTTTCGGTTA+AGG - chr4_4:50404139-50404158 Msa0686080:intron 40.0%
! TTTTGGACAGTACACTTTGG+TGG - chr4_4:50404181-50404200 Msa0686080:intron 40.0%
TTTGGACAGTACACTTTGGT+GGG - chr4_4:50404182-50404201 Msa0686080:intron 40.0%
! TAGGAGTTGCTTTCTATCGT+GGG - chr4_4:50404431-50404450 Msa0686080:intron 40.0%
TTGACAACCTTAACAACTGG+AGG - chr4_4:50404500-50404519 Msa0686080:intron 40.0%
TGACAACCTTAACAACTGGA+GGG - chr4_4:50404501-50404520 Msa0686080:intron 40.0%
GGAAACAAGATCGACATTGA+TGG - chr4_4:50404732-50404751 Msa0686080:intron 40.0%
AACAAGATCGACATTGATGG+TGG - chr4_4:50404735-50404754 Msa0686080:intron 40.0%
! ACAAGATCGACATTGATGGT+GGG - chr4_4:50404736-50404755 Msa0686080:intron 40.0%
CATGTTAGATAGCAAAAGGG+AGG + chr4_4:50404784-50404803 None:intergenic 40.0%
ACACATAAGTTCTTGTGCAC+AGG - chr4_4:50404885-50404904 Msa0686080:five_prime_UTR 40.0%
TGCACAGGTTTCAGAAAAGA+AGG - chr4_4:50404900-50404919 Msa0686080:five_prime_UTR 40.0%
AGGTTTCAGAAAAGAAGGCT+CGG - chr4_4:50404905-50404924 Msa0686080:five_prime_UTR 40.0%
! TTTGAGACATCTGCCAAAGA+AGG - chr4_4:50404961-50404980 Msa0686080:five_prime_UTR 40.0%
AGCATTCCAAACCATAGCAA+AGG - chr4_4:50404999-50405018 Msa0686080:five_prime_UTR 40.0%
GCATTTGTTTCCAAGAGACA+TGG + chr4_4:50405203-50405222 None:intergenic 40.0%
CATGAACCATTTGCCATGTT+AGG - chr4_4:50405255-50405274 Msa0686080:five_prime_UTR 40.0%
! TTTGGGATTTGTTCTCCATG+TGG - chr4_4:50405490-50405509 Msa0686080:five_prime_UTR 40.0%
AACAAGCACGAATTTCCACA+TGG + chr4_4:50405508-50405527 None:intergenic 40.0%
ATGAAACCTCAAGACAGCAA+CGG + chr4_4:50405669-50405688 None:intergenic 40.0%
TCTTCTGTGAGGTTTCTCCT+AGG - chr4_4:50401128-50401147 Msa0686080:three_prime_UTR 45.0%
AATAAGGGGAGAAAACCACG+GGG + chr4_4:50401266-50401285 None:intergenic 45.0%
GAATCTTCCACGGAATTTGG+TGG - chr4_4:50401730-50401749 Msa0686080:intron 45.0%
GGACAGGGAAGATATAATGC+AGG - chr4_4:50401761-50401780 Msa0686080:intron 45.0%
GCGCCATTAGATTTAACGAG+TGG + chr4_4:50401840-50401859 None:intergenic 45.0%
TCTGACACTGACACATGTGT+CGG + chr4_4:50402540-50402559 None:intergenic 45.0%
ATGTGTCAGTGTCAGACATG+CGG - chr4_4:50402544-50402563 Msa0686080:CDS 45.0%
ATACGACACATGTTGGTCAC+TGG - chr4_4:50402586-50402605 Msa0686080:intron 45.0%
TTGGTCACTGGACACATCTT+CGG - chr4_4:50402598-50402617 Msa0686080:intron 45.0%
!! GTTGTTGATGATTTGCAGGG+TGG - chr4_4:50402841-50402860 Msa0686080:intron 45.0%
!! TGTTGATGATTTGCAGGGTG+GGG - chr4_4:50402843-50402862 Msa0686080:intron 45.0%
GGTTAAGAAGTCAGCTCCAA+TGG + chr4_4:50403257-50403276 None:intergenic 45.0%
GCACCATTCACAAACCTGTA+AGG + chr4_4:50403311-50403330 None:intergenic 45.0%
GGCCTTTCATTTACAAGGCT+TGG + chr4_4:50403382-50403401 None:intergenic 45.0%
ACTAAATGGGGTCGGATACA+TGG + chr4_4:50403485-50403504 None:intergenic 45.0%
TGTATCCGACCCCATTTAGT+TGG - chr4_4:50403485-50403504 Msa0686080:intron 45.0%
CGACCCCATTTAGTTGGAAA+AGG - chr4_4:50403491-50403510 Msa0686080:intron 45.0%
GGCGTGTTTAATGTACACAC+AGG + chr4_4:50403747-50403766 None:intergenic 45.0%
GTGTGTACATTAAACACGCC+TGG - chr4_4:50403747-50403766 Msa0686080:intron 45.0%
! TTTGGAAACGGGCTTGTGTA+TGG - chr4_4:50403847-50403866 Msa0686080:intron 45.0%
! TTGGAAACGGGCTTGTGTAT+GGG - chr4_4:50403848-50403867 Msa0686080:intron 45.0%
! AAAGGCTCCTAAGAGTGCAA+CGG + chr4_4:50404131-50404150 None:intergenic 45.0%
! GCACTCTTAGGAGCCTTTTT+CGG - chr4_4:50404133-50404152 Msa0686080:intron 45.0%
TTGGACAGTACACTTTGGTG+GGG - chr4_4:50404183-50404202 Msa0686080:intron 45.0%
! TTGCAGATTTGGGATACAGC+TGG - chr4_4:50404388-50404407 Msa0686080:intron 45.0%
AGAAAGCAACTCCTAGGCTT+TGG + chr4_4:50404426-50404445 None:intergenic 45.0%
CACGATAGAAAGCAACTCCT+AGG + chr4_4:50404432-50404451 None:intergenic 45.0%
! CTAGGAGTTGCTTTCTATCG+TGG - chr4_4:50404430-50404449 Msa0686080:intron 45.0%
! AGGAGTTGCTTTCTATCGTG+GGG - chr4_4:50404432-50404451 Msa0686080:intron 45.0%
ATTCCTCCCTCCAGTTGTTA+AGG + chr4_4:50404510-50404529 None:intergenic 45.0%
GGAAAATTCTCTGGATCGGA+AGG + chr4_4:50404699-50404718 None:intergenic 45.0%
GGTTTCAGAAAAGAAGGCTC+GGG - chr4_4:50404906-50404925 Msa0686080:five_prime_UTR 45.0%
! TTTCAGGGCATCCTTTGCTA+TGG + chr4_4:50405013-50405032 None:intergenic 45.0%
CAAAGGATGCCCTGAAAAGT+GGG - chr4_4:50405016-50405035 Msa0686080:five_prime_UTR 45.0%
AAAGGATGCCCTGAAAAGTG+GGG - chr4_4:50405017-50405036 Msa0686080:five_prime_UTR 45.0%
!! TTCCTCTTCCCCACTTTTCA+GGG + chr4_4:50405028-50405047 None:intergenic 45.0%
ATGAGATGGTGTTCATTGGC+TGG - chr4_4:50405134-50405153 Msa0686080:five_prime_UTR 45.0%
! TGAGATGGTGTTCATTGGCT+GGG - chr4_4:50405135-50405154 Msa0686080:five_prime_UTR 45.0%
CAACATCAATTGTGTCTGGC+AGG + chr4_4:50405538-50405557 None:intergenic 45.0%
CTGCCAGACACAATTGATGT+TGG - chr4_4:50405536-50405555 Msa0686080:five_prime_UTR 45.0%
TTGGAAACAGCAATCAGCCA+AGG - chr4_4:50405555-50405574 Msa0686080:five_prime_UTR 45.0%
!! CGATTTGTTGGGTGCTTGTT+TGG - chr4_4:50405729-50405748 Msa0686080:five_prime_UTR 45.0%
!! ATAAATAAAATTGAAAATTA+TGG + chr4_4:50404612-50404631 None:intergenic 5.0%
TAGCAGCACGGATCGATTAG+AGG + chr4_4:50401500-50401519 None:intergenic 50.0%
! TTCCACGGAATTTGGTGGGT+TGG - chr4_4:50401735-50401754 Msa0686080:intron 50.0%
GGATCAATCAAGTCCCTCAC+CGG + chr4_4:50401878-50401897 None:intergenic 50.0%
!! AACGGTGGATTCTAGGGTGA+GGG + chr4_4:50401899-50401918 None:intergenic 50.0%
!! AAACGGTGGATTCTAGGGTG+AGG + chr4_4:50401900-50401919 None:intergenic 50.0%
GGAGGAAGAGTTAACCGCTT+TGG + chr4_4:50402128-50402147 None:intergenic 50.0%
!! AAGAGCGTTCTTCTACGGGA+AGG + chr4_4:50402164-50402183 None:intergenic 50.0%
GTCATCATTCTCGGTGACAG+CGG - chr4_4:50402188-50402207 Msa0686080:intron 50.0%
TCATCATTCTCGGTGACAGC+GGG - chr4_4:50402189-50402208 Msa0686080:intron 50.0%
TGTGTCAGTGTCAGACATGC+GGG - chr4_4:50402545-50402564 Msa0686080:CDS 50.0%
AGACATGCGGGACATTGTGT+AGG - chr4_4:50402557-50402576 Msa0686080:CDS 50.0%
GACAGCGATACGACACATGT+TGG - chr4_4:50402579-50402598 Msa0686080:intron 50.0%
CTGTGAAATAGCGGCTATGG+CGG - chr4_4:50403010-50403029 Msa0686080:intron 50.0%
CGGGCTTGTGTATGGGATTT+AGG - chr4_4:50403855-50403874 Msa0686080:intron 50.0%
!! CTAGGCTTTGGAATCTCTCC+TGG + chr4_4:50404414-50404433 None:intergenic 50.0%
CAGGAGAGATTCCAAAGCCT+AGG - chr4_4:50404412-50404431 Msa0686080:intron 50.0%
CAACCTTAACAACTGGAGGG+AGG - chr4_4:50404504-50404523 Msa0686080:intron 50.0%
!! TGATGGTGGGAACAGTAGAG+TGG - chr4_4:50404749-50404768 Msa0686080:intron 50.0%
! GGGCATCCTTTGCTATGGTT+TGG + chr4_4:50405008-50405027 None:intergenic 50.0%
GCAAAGGATGCCCTGAAAAG+TGG - chr4_4:50405015-50405034 Msa0686080:five_prime_UTR 50.0%
!! CTTCCTCTTCCCCACTTTTC+AGG + chr4_4:50405029-50405048 None:intergenic 50.0%
! GAGATGGTGTTCATTGGCTG+GGG - chr4_4:50405136-50405155 Msa0686080:five_prime_UTR 50.0%
! AGATGGTGTTCATTGGCTGG+GGG - chr4_4:50405137-50405156 Msa0686080:five_prime_UTR 50.0%
!! TTGGCTGGGGGAGCATATTT+TGG - chr4_4:50405149-50405168 Msa0686080:five_prime_UTR 50.0%
AGCAATCAGCCAAGGTCATC+AGG - chr4_4:50405563-50405582 Msa0686080:five_prime_UTR 50.0%
CACTCACATCCTGATGACCT+TGG + chr4_4:50405575-50405594 None:intergenic 50.0%
AGTTCTCTACTCTTGCCCCG+TGG - chr4_4:50401248-50401267 Msa0686080:three_prime_UTR 55.0%
CTCCAACCCACCAAATTCCG+TGG + chr4_4:50401740-50401759 None:intergenic 55.0%
!! CGGAATTTGGTGGGTTGGAG+AGG - chr4_4:50401740-50401759 Msa0686080:intron 55.0%
!! TTTGGTGGGTTGGAGAGGAC+AGG - chr4_4:50401745-50401764 Msa0686080:intron 55.0%
!! TTGGTGGGTTGGAGAGGACA+GGG - chr4_4:50401746-50401765 Msa0686080:intron 55.0%
!! GCGCTTTTTACGTGCTCCAC+CGG - chr4_4:50401856-50401875 Msa0686080:intron 55.0%
! TTTTACGTGCTCCACCGGTG+AGG - chr4_4:50401861-50401880 Msa0686080:intron 55.0%
TTTACGTGCTCCACCGGTGA+GGG - chr4_4:50401862-50401881 Msa0686080:intron 55.0%
TCAATCAAGTCCCTCACCGG+TGG + chr4_4:50401875-50401894 None:intergenic 55.0%
!! GAAGGCATCGTTCAGATCGG+AGG + chr4_4:50402146-50402165 None:intergenic 55.0%
!! CGGGAAGGCATCGTTCAGAT+CGG + chr4_4:50402149-50402168 None:intergenic 55.0%
! GATTTGGGATACAGCTGGCC+AGG - chr4_4:50404393-50404412 Msa0686080:intron 55.0%
CAGAAAAGAAGGCTCGGGCA+TGG - chr4_4:50404911-50404930 Msa0686080:five_prime_UTR 55.0%
TGCCCTGAAAAGTGGGGAAG+AGG - chr4_4:50405023-50405042 Msa0686080:five_prime_UTR 55.0%
GTCTAGCCGTTGCTGTCTTG+AGG - chr4_4:50405660-50405679 Msa0686080:five_prime_UTR 55.0%
CGGGCATGGTGTGCAGCAAA+AGG - chr4_4:50404925-50404944 Msa0686080:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 50401082 50405893 50401082 ID=Msa0686080;Name=Msa0686080
chr4_4 mRNA 50401082 50405893 50401082 ID=Msa0686080-mRNA-1;Parent=Msa0686080;Name=Msa0686080-mRNA-1;_AED=0.05;_eAED=0.05;_QI=1076|0.8|1|1|1|1|6|295|197
chr4_4 exon 50401082 50401443 50401082 ID=Msa0686080-mRNA-1:exon:12912;Parent=Msa0686080-mRNA-1
chr4_4 exon 50401923 50402068 50401923 ID=Msa0686080-mRNA-1:exon:12911;Parent=Msa0686080-mRNA-1
chr4_4 exon 50402205 50402285 50402205 ID=Msa0686080-mRNA-1:exon:12910;Parent=Msa0686080-mRNA-1
chr4_4 exon 50402435 50402581 50402435 ID=Msa0686080-mRNA-1:exon:12909;Parent=Msa0686080-mRNA-1
chr4_4 exon 50403660 50403759 50403660 ID=Msa0686080-mRNA-1:exon:12908;Parent=Msa0686080-mRNA-1
chr4_4 exon 50404765 50405893 50404765 ID=Msa0686080-mRNA-1:exon:12907;Parent=Msa0686080-mRNA-1
chr4_4 five_prime_UTR 50404818 50405893 50404818 ID=Msa0686080-mRNA-1:five_prime_utr;Parent=Msa0686080-mRNA-1
chr4_4 CDS 50404765 50404817 50404765 ID=Msa0686080-mRNA-1:cds;Parent=Msa0686080-mRNA-1
chr4_4 CDS 50403660 50403759 50403660 ID=Msa0686080-mRNA-1:cds;Parent=Msa0686080-mRNA-1
chr4_4 CDS 50402435 50402581 50402435 ID=Msa0686080-mRNA-1:cds;Parent=Msa0686080-mRNA-1
chr4_4 CDS 50402205 50402285 50402205 ID=Msa0686080-mRNA-1:cds;Parent=Msa0686080-mRNA-1
chr4_4 CDS 50401923 50402068 50401923 ID=Msa0686080-mRNA-1:cds;Parent=Msa0686080-mRNA-1
chr4_4 CDS 50401377 50401443 50401377 ID=Msa0686080-mRNA-1:cds;Parent=Msa0686080-mRNA-1
chr4_4 three_prime_UTR 50401082 50401376 50401082 ID=Msa0686080-mRNA-1:three_prime_utr;Parent=Msa0686080-mRNA-1
Gene Sequence

>Msa0686080

ATGCCTTCCCGTAGAAGAACGCTCTTGAAAGTCATCATTCTCGGTGACAGCGGATATGTGAATAAGAAGTTTAGTAATCAGTACAAGGCAACCATTGGAGCTGACTTCTTAACCAAAGAAGTGCAATTTGAAGATAGGCTTTTCACCTTACAGATTTGGGATACAGCTGGCCAGGAGAGATTCCAAAGCCTAGGAGTTGCTTTCTATCGTGGGGCTGATTGCTGTGTTCTTGTATACGATGTTAATTCAATGAAATCATTTGACAACCTTAACAACTGGAGGGAGGAATTTCTGATTCAAGCAAATCCTTCCGATCCAGAGAATTTTCCTTTTGTTGTTATAGGAAACAAGATCGACATTGATGGTGGGAACAGTAGAGTGGTTTCAGAAAAGAAGGCTCGGGCATGGTGTGCAGCAAAAGGAAATATCCCATATTTTGAGACATCTGCCAAAGAAGGTATTAATGTCGAAGAAGCATTCCAAACCATAGCAAAGGATGCCCTGAAAAGTGGGGAAGAGGAAGAATTATACCTGCCAGACACAATTGATGTTGGAAACAGCAATCAGCCAAGGTCATCAGGATGTGAGTGCTGA
Protein sequence

>Msa0686080

MPSRRRTLLKVIILGDSGYVNKKFSNQYKATIGADFLTKEVQFEDRLFTLQIWDTAGQERFQSLGVAFYRGADCCVLVYDVNSMKSFDNLNNWREEFLIQANPSDPENFPFVVIGNKIDIDGGNSRVVSEKKARAWCAAKGNIPYFETSAKEGINVEEAFQTIAKDALKSGEEEELYLPDTIDVGNSNQPRSSGCEC*