AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0235960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0235960 MtrunA17_Chr2g0284541 86.486 481 46 3 293 754 170 650 0.0 794
Msa0235960 MtrunA17_Chr2g0284501 72.780 518 107 7 267 753 153 667 0.0 689
Msa0235960 MtrunA17_Chr2g0284551 97.611 293 7 0 1 293 1 293 0.0 590
Msa0235960 MtrunA17_Chr5g0439061 40.138 289 163 4 455 733 435 723 9.26e-50 187
Msa0235960 MtrunA17_Chr3g0102001 35.756 344 193 8 437 760 413 748 1.51e-44 171
Msa0235960 MtrunA17_Chr2g0297701 34.507 284 177 5 458 733 280 562 5.83e-44 167
Msa0235960 MtrunA17_Chr5g0443001 31.057 454 267 12 290 736 183 597 1.68e-43 166
Msa0235960 MtrunA17_Chr2g0286601 34.551 301 187 6 457 748 390 689 1.15e-42 165
Msa0235960 MtrunA17_Chr3g0098481 33.333 294 180 6 455 734 34 325 2.23e-42 157
Msa0235960 MtrunA17_Chr5g0410201 31.833 311 186 7 441 734 18 319 6.99e-42 155
Msa0235960 MtrunA17_Chr2g0286551 32.344 337 219 5 411 741 326 659 1.16e-41 162
Msa0235960 MtrunA17_Chr5g0410171 27.389 471 284 14 291 736 290 727 2.20e-41 162
Msa0235960 MtrunA17_Chr3g0100741 35.433 254 158 5 452 700 303 555 2.49e-40 157
Msa0235960 MtrunA17_Chr5g0410271 31.511 311 187 7 441 734 17 318 3.96e-40 150
Msa0235960 MtrunA17_Chr2g0297691 34.602 289 174 5 456 733 400 684 6.97e-40 157
Msa0235960 MtrunA17_Chr2g0297481 35.088 285 173 6 456 733 185 464 6.82e-39 151
Msa0235960 MtrunA17_Chr2g0297491 32.632 285 184 4 456 733 87 370 1.16e-38 148
Msa0235960 MtrunA17_Chr2g0297681 33.910 289 176 5 456 733 398 682 1.52e-38 153
Msa0235960 MtrunA17_Chr3g0100301 42.784 194 103 6 542 730 209 399 4.68e-38 147
Msa0235960 MtrunA17_Chr2g0297651 34.926 272 163 5 472 733 416 683 2.01e-37 149
Msa0235960 MtrunA17_Chr5g0410341 26.946 501 313 16 266 736 262 739 2.28e-37 149
Msa0235960 MtrunA17_Chr5g0410461 34.866 261 158 4 450 701 161 418 9.11e-37 144
Msa0235960 MtrunA17_Chr6g0456141 30.033 303 204 3 430 727 69 368 1.05e-36 142
Msa0235960 MtrunA17_Chr3g0098301 33.333 294 180 6 455 734 100 391 1.46e-35 139
Msa0235960 MtrunA17_Chr6g0456101 28.507 442 277 10 320 746 324 741 4.66e-35 142
Msa0235960 MtrunA17_Chr3g0096941 32.000 250 164 5 456 700 308 556 5.97e-35 141
Msa0235960 MtrunA17_Chr3g0098531 31.801 261 165 5 486 734 18 277 2.20e-34 133
Msa0235960 MtrunA17_Chr5g0410581 30.714 280 168 7 444 709 491 758 6.82e-34 139
Msa0235960 MtrunA17_Chr2g0297711 29.137 278 189 4 464 733 242 519 8.14e-33 134
Msa0235960 MtrunA17_Chr6g0457101 26.667 435 267 11 320 742 298 692 9.73e-33 135
Msa0235960 MtrunA17_Chr5g0410371 31.724 290 180 6 457 732 1 286 1.52e-31 125
Msa0235960 MtrunA17_Chr5g0410191 35.714 182 116 1 553 733 2 183 4.63e-31 120
Msa0235960 MtrunA17_Chr5g0439051 39.011 182 110 1 553 733 20 201 1.14e-28 114
Msa0235960 MtrunA17_Chr2g0286561 34.091 220 134 5 541 757 3 214 1.97e-28 114
Msa0235960 MtrunA17_Chr5g0439081 36.098 205 124 4 553 755 16 215 4.08e-28 112
Msa0235960 MtrunA17_Chr5g0419741 38.286 175 102 2 101 274 123 292 1.32e-26 110
Msa0235960 MtrunA17_Chr5g0410541 39.568 139 84 0 567 705 37 175 9.19e-26 106
Msa0235960 MtrunA17_Chr3g0078241 41.143 175 99 3 98 269 284 457 9.96e-25 108
Msa0235960 MtrunA17_Chr2g0303951 36.325 234 138 5 54 280 240 469 7.88e-24 105
Msa0235960 MtrunA17_Chr3g0122541 40.972 144 83 2 109 251 280 422 2.02e-23 104
Msa0235960 MtrunA17_Chr5g0410451 34.667 150 95 2 566 712 54 203 3.08e-23 99.4
Msa0235960 MtrunA17_Chr3g0122521 37.086 151 93 2 102 251 12 161 3.68e-21 92.4
Msa0235960 MtrunA17_Chr5g0410181 37.008 127 79 1 611 736 1 127 1.04e-20 89.0
Msa0235960 MtrunA17_Chr3g0113611 38.961 154 75 4 109 243 73 226 1.80e-19 89.4
Msa0235960 MtrunA17_Chr2g0297521 48.148 81 42 0 548 628 4 84 6.03e-19 82.0
Msa0235960 MtrunA17_Chr2g0297611 41.975 81 47 0 567 647 9 89 4.41e-18 79.7
Msa0235960 MtrunA17_Chr5g0437111 41.558 154 71 6 109 243 99 252 1.21e-16 81.6
Msa0235960 MtrunA17_Chr5g0410511 32.386 176 104 5 567 730 16 188 6.50e-16 77.0
Msa0235960 MtrunA17_Chr1g0211541 27.036 307 207 10 443 737 207 508 1.34e-14 77.4
Msa0235960 MtrunA17_Chr3g0100921 33.333 132 82 4 603 730 3 132 1.44e-14 71.6
Msa0235960 MtrunA17_Chr5g0446321 31.210 157 89 2 91 244 172 312 2.03e-14 75.9
Msa0235960 MtrunA17_Chr1g0211531 23.529 255 181 5 460 702 243 495 1.38e-12 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0235960 AT5G45700.1 44.406 286 139 7 1 280 1 272 4.28e-58 199
Msa0235960 AT1G29780.1 55.814 172 75 1 107 277 49 220 6.54e-55 188
Msa0235960 AT1G29770.1 52.571 175 81 2 107 279 102 276 2.74e-53 186
Msa0235960 AT3G18670.2 28.542 480 301 12 259 730 183 628 4.76e-47 178
Msa0235960 AT3G18670.1 35.461 282 173 5 457 730 293 573 4.46e-44 168
Msa0235960 AT5G35810.1 33.770 305 185 6 444 733 22 324 1.09e-40 153
Msa0235960 AT5G04730.1 32.386 352 206 8 401 736 249 584 4.51e-38 150
Msa0235960 AT3G54065.1 41.573 178 96 2 539 709 8 184 6.79e-36 136
Msa0235960 AT5G04700.1 32.000 375 222 13 375 736 297 651 2.20e-35 143
Msa0235960 AT5G04690.3 36.508 252 140 7 494 732 379 623 1.96e-33 137
Msa0235960 AT5G04690.1 36.508 252 140 7 494 732 359 603 2.01e-33 137
Msa0235960 AT5G04690.2 36.508 252 140 7 494 732 393 637 2.27e-33 137
Msa0235960 AT5G04680.1 28.829 444 264 17 319 732 250 671 1.78e-30 128
Msa0235960 AT5G04680.3 32.955 264 151 9 490 732 370 628 4.72e-30 127
Msa0235960 AT5G04680.2 32.955 264 151 9 490 732 261 519 4.81e-30 125
Msa0235960 AT5G04680.4 32.955 264 151 9 490 732 384 642 5.57e-30 127
Msa0235960 AT5G11860.3 37.576 165 101 2 109 272 113 276 5.07e-25 106
Msa0235960 AT5G11860.5 37.576 165 101 2 109 272 113 276 5.07e-25 106
Msa0235960 AT5G11860.1 37.576 165 101 2 109 272 113 276 5.07e-25 106
Msa0235960 AT5G11860.4 37.576 165 101 2 109 272 113 276 5.07e-25 106
Msa0235960 AT5G11860.2 37.576 165 101 2 109 272 113 276 5.07e-25 106
Msa0235960 AT5G46410.3 39.884 173 102 2 109 280 282 453 5.40e-23 103
Msa0235960 AT5G46410.1 39.884 173 102 2 109 280 282 453 5.40e-23 103
Msa0235960 AT5G46410.2 39.884 173 102 2 109 280 285 456 5.41e-23 103
Msa0235960 AT4G18140.4 39.884 173 102 2 109 280 270 441 1.41e-21 99.0
Msa0235960 AT4G18140.6 39.884 173 102 2 109 280 270 441 1.41e-21 99.0
Msa0235960 AT4G18140.5 39.884 173 102 2 109 280 270 441 1.41e-21 99.0
Msa0235960 AT4G18140.1 40.606 165 96 2 109 272 270 433 4.62e-21 97.4
Msa0235960 AT4G18140.2 40.606 165 96 2 109 272 270 433 4.62e-21 97.4
Msa0235960 AT4G18140.3 40.606 165 96 2 109 272 270 433 6.35e-21 97.1
Msa0235960 AT1G55900.2 31.792 173 99 2 91 260 171 327 1.04e-17 86.3
Msa0235960 AT1G55900.1 31.792 173 99 2 91 260 171 327 1.18e-17 86.3
Msa0235960 AT3G55960.1 36.184 152 78 4 106 238 96 247 3.00e-15 77.8
Msa0235960 AT3G12360.1 24.848 330 186 7 457 737 236 552 3.13e-13 73.6
Msa0235960 AT3G12360.2 24.848 330 186 7 457 737 224 540 3.37e-13 73.6
Msa0235960 AT4G03470.1 25.652 230 143 5 459 664 344 569 5.61e-12 69.7

Find 137 sgRNAs with CRISPR-Local

Find 311 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTATTTACTGCAACAATTT+TGG 0.168186 2_2:-8806894 Msa0235960:CDS
AGAGCTTTGTATGATGAATT+TGG 0.213139 2_2:+8808732 None:intergenic
CTTGAATGCAGAAGGCATTT+TGG 0.217065 2_2:+8809360 None:intergenic
AGGGAAGCTTGTGAAGGATT+TGG 0.246705 2_2:-8811154 Msa0235960:CDS
GATGCTCGTGAATGGATAAA+AGG 0.265019 2_2:-8807214 Msa0235960:CDS
GGGAAGCTTGTGAAGGATTT+GGG 0.271030 2_2:-8811153 Msa0235960:CDS
CCGCGGTCGTTTGTGCCTCC+TGG 0.276528 2_2:+8807121 None:intergenic
TCCTATATTTGTAGTGTTCA+TGG 0.288165 2_2:-8807077 Msa0235960:CDS
TAGCAAAGAATGACAATTCA+TGG 0.298588 2_2:-8808470 Msa0235960:CDS
TTATTTCGCATCGGCTTTAT+AGG 0.299291 2_2:-8811197 Msa0235960:CDS
TACTTATACACTGTTGCTTA+TGG 0.315146 2_2:-8808355 Msa0235960:CDS
TTCAAAGCTTCTCACCAAAC+TGG 0.321603 2_2:-8811625 Msa0235960:CDS
GAGCTTTGTATGATGAATTT+GGG 0.327772 2_2:+8808733 None:intergenic
GCAACTCCTCTTGCAATTGC+AGG 0.330340 2_2:+8808085 None:intergenic
GCTTAAGGAGTATGCGAGTT+TGG 0.339130 2_2:-8811247 Msa0235960:CDS
GGTTGATTCGAAAATGAAAC+TGG 0.340128 2_2:+8811084 None:intergenic
AGATGTGGAAACTGGCAAAA+AGG 0.344936 2_2:-8808795 Msa0235960:CDS
GTTAATGTCTTTGATTGATT+TGG 0.348380 2_2:+8811731 None:intergenic
TATGCATCATCTTGATAATC+AGG 0.357973 2_2:+8808854 None:intergenic
TAGTAATATCATTATCTTGA+AGG 0.373856 2_2:+8808828 None:intergenic
TATTTCGCATCGGCTTTATA+GGG 0.378274 2_2:-8811196 Msa0235960:CDS
ACATTCGGAAAATTTCTAAC+TGG 0.379499 2_2:+8808217 None:intergenic
GCAGCGACGACGTTCTTTAT+CGG 0.381223 2_2:+8811684 None:intergenic
CGAAGAGTTCCCTTGCAGTT+TGG 0.384496 2_2:+8807263 None:intergenic
CATCTGATCAAAGGCGTGTC+TGG 0.397775 2_2:-8810907 Msa0235960:intron
CACATCACCTTGTGAGTTGT+GGG 0.403121 2_2:-8806993 Msa0235960:CDS
AATCATAGAGATAGTAGAAT+TGG 0.404231 2_2:-8808319 Msa0235960:CDS
AAAAGAGAAGATGTGGAAAC+TGG 0.411277 2_2:-8808803 Msa0235960:CDS
ATAGGATGATAGAGTAATCT+TGG 0.413261 2_2:+8807094 None:intergenic
TTCTATGGCCACAACCATCT+TGG 0.414784 2_2:-8806915 Msa0235960:CDS
TGGACCGGTTGGACCGGAAC+GGG 0.415871 2_2:-8811221 Msa0235960:CDS
TGTGGGTAGGGATTTGAAGA+AGG 0.416733 2_2:-8811127 Msa0235960:CDS
TTGCGATCCTCCTTTAAAAT+CGG 0.419403 2_2:+8808119 None:intergenic
ATGCTTGTGATCGTGAGCTT+TGG 0.419497 2_2:-8811026 Msa0235960:CDS
TTAATCAAAATCGCATTCTC+AGG 0.423966 2_2:+8811063 None:intergenic
ACCATGAACACTACAAATAT+AGG 0.425279 2_2:+8807076 None:intergenic
GTAGTCCGGCCGGTGATTGA+TGG 0.425963 2_2:-8811366 Msa0235960:CDS
TGTAGCACTTGCAAGTTCAT+TGG 0.428772 2_2:-8807044 Msa0235960:CDS
CTCGTCTCAGTTCTTCAAAT+CGG 0.429741 2_2:-8811596 Msa0235960:CDS
TTTGAAGAAGGTTGTTATTG+TGG 0.430715 2_2:-8811115 Msa0235960:CDS
CGCGGTCGTTTGTGCCTCCT+GGG 0.434459 2_2:+8807122 None:intergenic
TCTTGATTTAGATGAAACTT+TGG 0.436405 2_2:-8811430 Msa0235960:CDS
AAACCTGCCATTAGTTTCCT+AGG 0.438939 2_2:+8806953 None:intergenic
TTCCACCTTCAATATCAAAT+CGG 0.441277 2_2:-8811464 Msa0235960:CDS
TGTTCCTCAAGTTGATCAAA+AGG 0.442089 2_2:-8806726 Msa0235960:CDS
TTGCTTATGGCAGCTTGTAA+TGG 0.447918 2_2:-8808342 Msa0235960:CDS
GTGTATAAGTAATAGCTTTG+TGG 0.454421 2_2:+8808367 None:intergenic
TCAAGTTAGATAAGAGCAAA+TGG 0.454803 2_2:-8806865 Msa0235960:CDS
AAAATGGTGAGACATCTTGC+TGG 0.459972 2_2:-8808183 Msa0235960:CDS
TGTCGCGACAGTTGTCTTTG+CGG 0.463671 2_2:-8807158 Msa0235960:CDS
GAGACGAGTGAGTCCCAGTT+TGG 0.464227 2_2:+8811611 None:intergenic
TAAACCCGTTCCGGTCCAAC+CGG 0.468555 2_2:+8811217 None:intergenic
TTCTGAAAGATGCTCGTGAA+TGG 0.471142 2_2:-8807222 Msa0235960:CDS
GATGCGAAATAAACCCGTTC+CGG 0.471268 2_2:+8811208 None:intergenic
ACTTTATATGTTGCCATGAA+AGG 0.473275 2_2:-8806785 Msa0235960:CDS
ACCGGGGATTGATGAATTGT+TGG 0.482144 2_2:-8811313 Msa0235960:CDS
GTGGACCGGTTGGACCGGAA+CGG 0.486922 2_2:-8811222 Msa0235960:CDS
TGCCATAAGACATCCTTTCA+TGG 0.486948 2_2:+8806772 None:intergenic
CAGACATGCTCAATTGATTG+AGG 0.487698 2_2:+8808282 None:intergenic
TGCCATGAAAGGATGTCTTA+TGG 0.490498 2_2:-8806774 Msa0235960:CDS
CTTTGAAATGCGGCGGTGGA+TGG 0.490657 2_2:+8811641 None:intergenic
GATGTCTTATGGCACTCCTT+AGG 0.491916 2_2:-8806763 Msa0235960:CDS
TCACATCACCTTGTGAGTTG+TGG 0.495251 2_2:-8806994 Msa0235960:CDS
ATGGACAGAACAGTTTGTAT+AGG 0.496757 2_2:-8808429 Msa0235960:CDS
ATGCTCGTGAATGGATAAAA+GGG 0.500318 2_2:-8807213 Msa0235960:CDS
TGCAAGTTCATTGGCTTCAG+TGG 0.501558 2_2:-8807035 Msa0235960:CDS
GGAGTATGCGAGTTTGGTTG+TGG 0.507228 2_2:-8811241 Msa0235960:CDS
ATTTGTAGTGTTCATGGTCA+TGG 0.508532 2_2:-8807071 Msa0235960:CDS
TGCAATTGCAGGGCATAACC+AGG 0.508562 2_2:+8808096 None:intergenic
GTCTCTACCTAGGAAACTAA+TGG 0.510880 2_2:-8806960 Msa0235960:CDS
TTCTACGTGTTGAAGCGACC+GGG 0.513582 2_2:-8811330 Msa0235960:CDS
TTGTGAATGATGTAATGATA+AGG 0.515494 2_2:+8807298 None:intergenic
TAGGGATTCGTGTAGGAATG+TGG 0.519949 2_2:-8811178 Msa0235960:CDS
ATTCATCATACAAAGCTCTC+AGG 0.520254 2_2:-8808728 Msa0235960:intron
TCAAAGCTTCTCACCAAACT+GGG 0.520599 2_2:-8811624 Msa0235960:CDS
AAATCAAATTTCTCCGGCGG+TGG 0.522620 2_2:+8811390 None:intergenic
TCACAAGCATCATCAACAAA+AGG 0.529060 2_2:+8811039 None:intergenic
AGCGTTGAAATATGAGGTGG+TGG 0.529684 2_2:-8811283 Msa0235960:CDS
CGTGAGCTTTGGAAATTGAG+AGG 0.530120 2_2:-8811015 Msa0235960:CDS
TTTCTACGTGTTGAAGCGAC+CGG 0.530989 2_2:-8811331 Msa0235960:CDS
GCGGCAGCTTACACTGTCCC+AGG 0.531643 2_2:-8807139 Msa0235960:CDS
TGGTTGTGGACCGGTTGGAC+CGG 0.534625 2_2:-8811227 Msa0235960:CDS
ATGCGAGTTTGGTTGTGGAC+CGG 0.538407 2_2:-8811236 Msa0235960:CDS
TGAAATGTGTTGATTTCTTG+AGG 0.539909 2_2:+8811553 None:intergenic
TGTTGCAGTAAATACCAAGA+TGG 0.543077 2_2:+8806901 None:intergenic
GAAACTTAGTTCAATAGCAA+TGG 0.547491 2_2:-8808448 Msa0235960:CDS
TTTAAAGGAGGATCGCAACC+TGG 0.555704 2_2:-8808114 Msa0235960:CDS
CTTTAAAATCGGCAATATTG+CGG 0.556459 2_2:+8808130 None:intergenic
AATTGCAAGAGGAGTTGCAT+TGG 0.556764 2_2:-8808080 Msa0235960:CDS
CAATCTTCTTAAGACTCCTA+AGG 0.556847 2_2:+8806747 None:intergenic
GTTTGACTCTTGAATGCAGA+AGG 0.559525 2_2:+8809352 None:intergenic
TTCAAGAGTCAAACCCAGAT+GGG 0.561047 2_2:-8809343 Msa0235960:CDS
CAACTCCTCTTGCAATTGCA+GGG 0.566425 2_2:+8808086 None:intergenic
AGTAAATACCAAGATGGTTG+TGG 0.567450 2_2:+8806907 None:intergenic
ACTACAAAATCAAATTTCTC+CGG 0.568154 2_2:+8811384 None:intergenic
GGAACGGGTTTATTTCGCAT+CGG 0.571199 2_2:-8811206 Msa0235960:CDS
GATTCGTGTAGGAATGTGGA+AGG 0.572672 2_2:-8811174 Msa0235960:CDS
AAGGTGATGTGAGGATTGAA+AGG 0.575166 2_2:+8807005 None:intergenic
ATTCAAGAGTCAAACCCAGA+TGG 0.576517 2_2:-8809344 Msa0235960:CDS
TCGTGTTCACATCTGATCAA+AGG 0.577857 2_2:-8810916 Msa0235960:CDS
GAAGCTTTGAAATGCGGCGG+TGG 0.579230 2_2:+8811637 None:intergenic
CTACCTAGGAAACTAATGGC+AGG 0.580520 2_2:-8806956 Msa0235960:CDS
ATCGGTTCACCATCAATCAC+CGG 0.589525 2_2:+8811357 None:intergenic
TCCAACAATTCATCAATCCC+CGG 0.590132 2_2:+8811312 None:intergenic
TGGTGAGAAGCTTTGAAATG+CGG 0.590878 2_2:+8811631 None:intergenic
CTAAAATCCCACAACTCACA+AGG 0.593605 2_2:+8806986 None:intergenic
TTTGTATGATGAATTTGGGG+TGG 0.594459 2_2:+8808737 None:intergenic
TGTGGAAGGGAAGCTTGTGA+AGG 0.595546 2_2:-8811160 Msa0235960:CDS
ACGACAACAACCAAACTGCA+AGG 0.597263 2_2:-8807273 Msa0235960:CDS
TCTACGTGTTGAAGCGACCG+GGG 0.598046 2_2:-8811329 Msa0235960:CDS
TTCCGATTTGATATTGAAGG+TGG 0.602664 2_2:+8811462 None:intergenic
TGCTTCCGTTGAGTTTGATG+TGG 0.602934 2_2:+8808761 None:intergenic
CGACAACAACCAAACTGCAA+GGG 0.606131 2_2:-8807272 Msa0235960:CDS
TGAGCAGAACATACTGTACA+TGG 0.607287 2_2:-8808253 Msa0235960:CDS
GATGGAAGAAAGAACGGCGG+AGG 0.608748 2_2:+8811659 None:intergenic
CAAAGACAACTGTCGCGACA+AGG 0.614573 2_2:+8807161 None:intergenic
TTCAACACGTAGAAATCCAT+CGG 0.617017 2_2:+8811339 None:intergenic
AAGACATTAACCGCCGTCGT+TGG 0.617171 2_2:-8811719 Msa0235960:CDS
GGTGATTGATGGTGAACCGA+TGG 0.621208 2_2:-8811355 Msa0235960:CDS
GGCTTTATAGGGATTCGTGT+AGG 0.622796 2_2:-8811185 Msa0235960:CDS
GCGGTGGATGGAAGAAAGAA+CGG 0.625468 2_2:+8811653 None:intergenic
TTATGCCCTGCAATTGCAAG+AGG 0.636536 2_2:-8808091 Msa0235960:CDS
AGCGTTAGCGTTGAAATATG+AGG 0.636943 2_2:-8811289 Msa0235960:CDS
AGCTTTGTATGATGAATTTG+GGG 0.637650 2_2:+8808734 None:intergenic
GGCCATAGAAAAGAACAAGA+AGG 0.647299 2_2:+8806928 None:intergenic
ATGAACCACATCAAACTCAA+CGG 0.649423 2_2:-8808766 Msa0235960:CDS
GTTCACCATCAATCACCGGC+CGG 0.662293 2_2:+8811361 None:intergenic
ACAACTCACAAGGTGATGTG+AGG 0.665806 2_2:+8806996 None:intergenic
ACAAAATCAAATTTCTCCGG+CGG 0.674025 2_2:+8811387 None:intergenic
AGAGTAATCTTGGTAAGCCG+CGG 0.674960 2_2:+8807104 None:intergenic
ATTCGTGTAGGAATGTGGAA+GGG 0.678685 2_2:-8811173 Msa0235960:CDS
GAGTTTGGTTGTGGACCGGT+TGG 0.681067 2_2:-8811232 Msa0235960:CDS
TGAGAAGCTTTGAAATGCGG+CGG 0.682036 2_2:+8811634 None:intergenic
CCAGGAGGCACAAACGACCG+CGG 0.691713 2_2:-8807121 Msa0235960:CDS
GCAGCTTACACTGTCCCAGG+AGG 0.705291 2_2:-8807136 Msa0235960:CDS
GTTAGCGTTGAAATATGAGG+TGG 0.719947 2_2:-8811286 Msa0235960:CDS
GTGGATGGAAGAAAGAACGG+CGG 0.776465 2_2:+8811656 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTCTTATATGAAAAAT+TGG - chr2_2:8808897-8808916 Msa0235960:intron 10.0%
!! TGAAAGATAGAAAAAAAAAA+CGG - chr2_2:8807710-8807729 Msa0235960:intron 15.0%
!! ATAATATCAAATATATCACA+TGG + chr2_2:8808049-8808068 None:intergenic 15.0%
!! ATGTGATATATTTGATATTA+TGG - chr2_2:8808048-8808067 Msa0235960:intron 15.0%
!! TCTTTCAATTTATTAGAAAA+AGG - chr2_2:8808842-8808861 Msa0235960:CDS 15.0%
!! AAGAAAAAATTCATATCTAT+TGG + chr2_2:8808886-8808905 None:intergenic 15.0%
!!! TTTTTTTTTAATGGACTTAA+AGG + chr2_2:8809232-8809251 None:intergenic 15.0%
!!! ATTTTTAACTCTTTAAGAAA+TGG + chr2_2:8809320-8809339 None:intergenic 15.0%
!! CATTAATAGTAATTATGTAT+TGG + chr2_2:8809350-8809369 None:intergenic 15.0%
!! CAATACATAATTACTATTAA+TGG - chr2_2:8809348-8809367 Msa0235960:CDS 15.0%
!!! TTGTATATCTAGTATTTTAT+AGG + chr2_2:8809415-8809434 None:intergenic 15.0%
!! TTCATCTAAATCAAGAAAAA+CGG + chr2_2:8807040-8807059 None:intergenic 20.0%
!! GAAAGATAGAAAAAAAAAAC+GGG - chr2_2:8807711-8807730 Msa0235960:intron 20.0%
!! GTTTATAATTTCCCTAAATT+TGG - chr2_2:8807861-8807880 Msa0235960:intron 20.0%
!! TATTTCTTTGCTTATACATA+TGG + chr2_2:8808662-8808681 None:intergenic 20.0%
!! TTTCTTATATGAAAAATTGG+AGG - chr2_2:8808900-8808919 Msa0235960:intron 20.0%
!! TTCTTATATGAAAAATTGGA+GGG - chr2_2:8808901-8808920 Msa0235960:intron 20.0%
!!! ATTTCTTATTTCTTTCTCTT+TGG - chr2_2:8808962-8808981 Msa0235960:intron 20.0%
!!! TTTCTTATTTCTTTCTCTTT+GGG - chr2_2:8808963-8808982 Msa0235960:intron 20.0%
!!! ACAGCTTTATTTTTATTGAA+AGG - chr2_2:8809032-8809051 Msa0235960:intron 20.0%
!!! CAGCTTTATTTTTATTGAAA+GGG - chr2_2:8809033-8809052 Msa0235960:intron 20.0%
!!! TTATTTTTATTGAAAGGGTA+CGG - chr2_2:8809038-8809057 Msa0235960:intron 20.0%
!! TAGTAATATCATTATCTTGA+AGG + chr2_2:8809649-8809668 None:intergenic 20.0%
!!! TTTTGGTTGAAAATTTCTTA+TGG + chr2_2:8809825-8809844 None:intergenic 20.0%
!!! TAGAAAAACTCATTTTTCAT+TGG - chr2_2:8810668-8810687 Msa0235960:intron 20.0%
!! TTACAGCAGAAAATTTAAAT+TGG - chr2_2:8810922-8810941 Msa0235960:CDS 20.0%
!! CACATCTAATAAATAGAATT+AGG + chr2_2:8811059-8811078 None:intergenic 20.0%
! GTTAATGTCTTTGATTGATT+TGG + chr2_2:8806746-8806765 None:intergenic 25.0%
! TCTTGATTTAGATGAAACTT+TGG - chr2_2:8807044-8807063 Msa0235960:CDS 25.0%
! ACTACAAAATCAAATTTCTC+CGG + chr2_2:8807093-8807112 None:intergenic 25.0%
!!! AGAAATTTGATTTTGTAGTC+CGG - chr2_2:8807094-8807113 Msa0235960:CDS 25.0%
!!! TGATTGTTTTGATGATATGA+GGG - chr2_2:8807497-8807516 Msa0235960:intron 25.0%
! AAATTGTGAAAGAATGAAGT+TGG - chr2_2:8807656-8807675 Msa0235960:intron 25.0%
! GAGAAATCAAATAAGTGAAA+TGG + chr2_2:8807993-8808012 None:intergenic 25.0%
!! TTATTTGATTTCTCAGTTCA+TGG - chr2_2:8807999-8808018 Msa0235960:intron 25.0%
!! AACTAGAGTTTTCTTTAACT+CGG - chr2_2:8808300-8808319 Msa0235960:CDS 25.0%
! TCCTCTATTATTTGCTTTAT+TGG - chr2_2:8808323-8808342 Msa0235960:CDS 25.0%
! TCCAATAAAGCAAATAATAG+AGG + chr2_2:8808327-8808346 None:intergenic 25.0%
! CTTTATCTACGGAAATATAA+TGG + chr2_2:8808385-8808404 None:intergenic 25.0%
! TACAAGTCTGTCATATATTA+GGG + chr2_2:8808488-8808507 None:intergenic 25.0%
! TTTCTTCAATTTAACCTACA+TGG + chr2_2:8808631-8808650 None:intergenic 25.0%
! TGTAGGTTAAATTGAAGAAA+TGG - chr2_2:8808631-8808650 Msa0235960:intron 25.0%
! TATAAGCAAAGAAATATGAC+TGG - chr2_2:8808666-8808685 Msa0235960:intron 25.0%
! AACAAAAAGCTATTGAATAG+TGG - chr2_2:8808716-8808735 Msa0235960:intron 25.0%
!!! CTATTGAATAGTGGATTTAA+AGG - chr2_2:8808725-8808744 Msa0235960:intron 25.0%
! AAAGATTTCAAAAAGACAGA+AGG + chr2_2:8808827-8808846 None:intergenic 25.0%
!! TAGAAAAAGGTACATGTATT+TGG - chr2_2:8808855-8808874 Msa0235960:CDS 25.0%
!! AGGATATATGAAATTTTCGA+AGG + chr2_2:8809159-8809178 None:intergenic 25.0%
!!! AGGCACATGTTTTTTTTTAA+TGG + chr2_2:8809241-8809260 None:intergenic 25.0%
! GTTTGTTTAATTATAGGTCA+AGG + chr2_2:8809390-8809409 None:intergenic 25.0%
! TAGGCTGTTTGTTTAATTAT+AGG + chr2_2:8809396-8809415 None:intergenic 25.0%
!! AAATTTTCAACCAAAATTGC+AGG - chr2_2:8809829-8809848 Msa0235960:intron 25.0%
!! CATTTTGTTCATGTTTAGAA+TGG - chr2_2:8809937-8809956 Msa0235960:intron 25.0%
!!! GGAAGAGTATTAAAAAACTT+TGG - chr2_2:8809958-8809977 Msa0235960:intron 25.0%
! AATCATAGAGATAGTAGAAT+TGG - chr2_2:8810155-8810174 Msa0235960:intron 25.0%
!! TTTTCCGAATGTTGAAAAAA+CGG - chr2_2:8810268-8810287 Msa0235960:intron 25.0%
!!! TTTTCCGTTTTTTCAACATT+CGG + chr2_2:8810275-8810294 None:intergenic 25.0%
! AATGTTGAAAAAACGGAAAA+TGG - chr2_2:8810275-8810294 Msa0235960:intron 25.0%
! TAGGTTAAGACAACTTAAAA+TGG - chr2_2:8810525-8810544 Msa0235960:intron 25.0%
! TAAGACAACTTAAAATGGTA+AGG - chr2_2:8810530-8810549 Msa0235960:intron 25.0%
! CAAAAGTAGTAAGTTTATCT+TGG + chr2_2:8810618-8810637 None:intergenic 25.0%
!! CTTTTTAAACTATGCAAGTT+TGG - chr2_2:8810639-8810658 Msa0235960:intron 25.0%
!!! AAAAACTCATTTTTCATTGG+TGG - chr2_2:8810671-8810690 Msa0235960:intron 25.0%
! TTGTGAATGATGTAATGATA+AGG + chr2_2:8811179-8811198 None:intergenic 25.0%
!! TGAAATGTGTTGATTTCTTG+AGG + chr2_2:8806924-8806943 None:intergenic 30.0%
TTCCACCTTCAATATCAAAT+CGG - chr2_2:8807010-8807029 Msa0235960:CDS 30.0%
!! GTTTTCCGATTTGATATTGA+AGG + chr2_2:8807018-8807037 None:intergenic 30.0%
! TTTGAAGAAGGTTGTTATTG+TGG - chr2_2:8807359-8807378 Msa0235960:intron 30.0%
TTAATCAAAATCGCATTCTC+AGG + chr2_2:8807414-8807433 None:intergenic 30.0%
!!! AAATTGAGAGGCTTTTTTGA+TGG - chr2_2:8807471-8807490 Msa0235960:intron 30.0%
!!! AATTGAGAGGCTTTTTTGAT+GGG - chr2_2:8807472-8807491 Msa0235960:intron 30.0%
!!! GTGATTGTTTTGATGATATG+AGG - chr2_2:8807496-8807515 Msa0235960:intron 30.0%
!! AATGTAATGATGTGGATTAG+TGG - chr2_2:8807739-8807758 Msa0235960:intron 30.0%
! GTTAAGCATTGCCAAATTTA+GGG + chr2_2:8807875-8807894 None:intergenic 30.0%
! TGTTAAGCATTGCCAAATTT+AGG + chr2_2:8807876-8807895 None:intergenic 30.0%
ACTCTTACTAGTCAAAGTAA+AGG + chr2_2:8807954-8807973 None:intergenic 30.0%
! GACATGGATTAAAAACAAGT+TGG - chr2_2:8808021-8808040 Msa0235960:intron 30.0%
AATCAATTATGCAGATTGCA+GGG - chr2_2:8808111-8808130 Msa0235960:CDS 30.0%
TTACAACTTACAAGGAGATT+AGG + chr2_2:8808231-8808250 None:intergenic 30.0%
TATCACTTTCATTGATAAGC+TGG + chr2_2:8808281-8808300 None:intergenic 30.0%
GTACAAGTCTGTCATATATT+AGG + chr2_2:8808489-8808508 None:intergenic 30.0%
TTAACTAATTACACCACTAG+TGG + chr2_2:8808584-8808603 None:intergenic 30.0%
CAAAGAAATATGACTGGAAA+GGG - chr2_2:8808672-8808691 Msa0235960:intron 30.0%
GGAAAGAAAAACCAAAGAAT+TGG + chr2_2:8808806-8808825 None:intergenic 30.0%
AAAGTTATCAGAGATTTACG+AGG + chr2_2:8809179-8809198 None:intergenic 30.0%
TGAAGTCTAGAGATAGAATT+AGG + chr2_2:8809468-8809487 None:intergenic 30.0%
AAGGAGAAGCTATTAGTAAT+AGG + chr2_2:8809520-8809539 None:intergenic 30.0%
!! AATCTTTTAATTGTCGTCGA+AGG + chr2_2:8809539-8809558 None:intergenic 30.0%
TACCAACTATAGCTAATAGT+TGG - chr2_2:8809578-8809597 Msa0235960:intron 30.0%
TTCCAACTATTAGCTATAGT+TGG + chr2_2:8809583-8809602 None:intergenic 30.0%
! TATGCATCATCTTGATAATC+AGG + chr2_2:8809623-8809642 None:intergenic 30.0%
TACTAGAAAAAGAGAAGATG+TGG - chr2_2:8809664-8809683 Msa0235960:intron 30.0%
! AGCTTTGTATGATGAATTTG+GGG + chr2_2:8809743-8809762 None:intergenic 30.0%
! GAGCTTTGTATGATGAATTT+GGG + chr2_2:8809744-8809763 None:intergenic 30.0%
! AGAGCTTTGTATGATGAATT+TGG + chr2_2:8809745-8809764 None:intergenic 30.0%
ATAATCAAAGCTTACACCTT+TGG + chr2_2:8809859-8809878 None:intergenic 30.0%
TAGCAAAGAATGACAATTCA+TGG - chr2_2:8810004-8810023 Msa0235960:intron 30.0%
GAAACTTAGTTCAATAGCAA+TGG - chr2_2:8810026-8810045 Msa0235960:intron 30.0%
! GTGTATAAGTAATAGCTTTG+TGG + chr2_2:8810110-8810129 None:intergenic 30.0%
! TACTTATACACTGTTGCTTA+TGG - chr2_2:8810119-8810138 Msa0235960:intron 30.0%
ACATTCGGAAAATTTCTAAC+TGG + chr2_2:8810260-8810279 None:intergenic 30.0%
!! CTTTAAAATCGGCAATATTG+CGG + chr2_2:8810347-8810366 None:intergenic 30.0%
!!! AATATTGCCGATTTTAAAGG+AGG - chr2_2:8810348-8810367 Msa0235960:intron 30.0%
TGGAATGCATGCATTAAATT+AGG + chr2_2:8810472-8810491 None:intergenic 30.0%
TACATAGTATAATGTGCACA+AGG - chr2_2:8810591-8810610 Msa0235960:intron 30.0%
AGTAATCTGCTTGAATTTGT+TGG - chr2_2:8810763-8810782 Msa0235960:intron 30.0%
AATCTTAAAATCATAGCCGT+CGG + chr2_2:8810797-8810816 None:intergenic 30.0%
CAGCAGAAAATTTAAATTGG+AGG - chr2_2:8810925-8810944 Msa0235960:CDS 30.0%
TTACTAGGAATTGAATAGAG+AGG + chr2_2:8811030-8811049 None:intergenic 30.0%
! AGAATTAGGTTAGTGTTACT+AGG + chr2_2:8811045-8811064 None:intergenic 30.0%
ATAGGATGATAGAGTAATCT+TGG + chr2_2:8811383-8811402 None:intergenic 30.0%
TCCTATATTTGTAGTGTTCA+TGG - chr2_2:8811397-8811416 Msa0235960:CDS 30.0%
ACCATGAACACTACAAATAT+AGG + chr2_2:8811401-8811420 None:intergenic 30.0%
! GGTATTTACTGCAACAATTT+TGG - chr2_2:8811580-8811599 Msa0235960:CDS 30.0%
TCAAGTTAGATAAGAGCAAA+TGG - chr2_2:8811609-8811628 Msa0235960:CDS 30.0%
ACTTTATATGTTGCCATGAA+AGG - chr2_2:8811689-8811708 Msa0235960:CDS 30.0%
! AAAAAGCAGGGTTTTGCAAA+CGG + chr2_2:8806965-8806984 None:intergenic 35.0%
! TTCCGATTTGATATTGAAGG+TGG + chr2_2:8807015-8807034 None:intergenic 35.0%
ACAAAATCAAATTTCTCCGG+CGG + chr2_2:8807090-8807109 None:intergenic 35.0%
TTCAACACGTAGAAATCCAT+CGG + chr2_2:8807138-8807157 None:intergenic 35.0%
!! ATATGAGGTGGTGGTTTTTA+CGG - chr2_2:8807200-8807219 Msa0235960:CDS 35.0%
!! TTATTTCGCATCGGCTTTAT+AGG - chr2_2:8807277-8807296 Msa0235960:CDS 35.0%
!! TATTTCGCATCGGCTTTATA+GGG - chr2_2:8807278-8807297 Msa0235960:CDS 35.0%
!! GGTTGATTCGAAAATGAAAC+TGG + chr2_2:8807393-8807412 None:intergenic 35.0%
TCACAAGCATCATCAACAAA+AGG + chr2_2:8807438-8807457 None:intergenic 35.0%
GTCAAAGTAAAGGTAAACTC+TGG + chr2_2:8807944-8807963 None:intergenic 35.0%
GAATCAATTATGCAGATTGC+AGG - chr2_2:8808110-8808129 Msa0235960:CDS 35.0%
! TTCCCTTTTTAATGTTCCAC+AGG - chr2_2:8808178-8808197 Msa0235960:CDS 35.0%
CTCCTGTGGAACATTAAAAA+GGG + chr2_2:8808183-8808202 None:intergenic 35.0%
TCTCCTGTGGAACATTAAAA+AGG + chr2_2:8808184-8808203 None:intergenic 35.0%
TCCACAGGAGAAAAGATAAT+AGG - chr2_2:8808193-8808212 Msa0235960:CDS 35.0%
! TCCTATTATCTTTTCTCCTG+TGG + chr2_2:8808197-8808216 None:intergenic 35.0%
AACTTACAAGGAGATTAGGA+GGG + chr2_2:8808227-8808246 None:intergenic 35.0%
CTTCACAGTTACAACTTACA+AGG + chr2_2:8808239-8808258 None:intergenic 35.0%
CAATACGATCAGTTGTTACT+AGG + chr2_2:8808351-8808370 None:intergenic 35.0%
CGTTGTTACGTCTTTATCTA+CGG + chr2_2:8808396-8808415 None:intergenic 35.0%
AAGACGTAACAACGAATGAA+AGG - chr2_2:8808402-8808421 Msa0235960:CDS 35.0%
TATGTTATTCCTTCCACTAG+TGG - chr2_2:8808568-8808587 Msa0235960:intron 35.0%
GCAAAGAAATATGACTGGAA+AGG - chr2_2:8808671-8808690 Msa0235960:intron 35.0%
AATGACAGCAATGTGAAGAA+TGG + chr2_2:8808781-8808800 None:intergenic 35.0%
ATTCTTCACATTGCTGTCAT+TGG - chr2_2:8808780-8808799 Msa0235960:CDS 35.0%
! TCTCTTTGGGAAGAAAAATG+AGG - chr2_2:8808976-8808995 Msa0235960:intron 35.0%
TTTGGGAAGAAAAATGAGGT+TGG - chr2_2:8808980-8808999 Msa0235960:intron 35.0%
! TGAACTAACTTGTGACTTGA+AGG + chr2_2:8809261-8809280 None:intergenic 35.0%
CATAGATCCTAAGTGATAAG+AGG - chr2_2:8809494-8809513 Msa0235960:intron 35.0%
TAATAGGCCTCTTATCACTT+AGG + chr2_2:8809504-8809523 None:intergenic 35.0%
AAAAGAGAAGATGTGGAAAC+TGG - chr2_2:8809671-8809690 Msa0235960:intron 35.0%
ATGAACCACATCAAACTCAA+CGG - chr2_2:8809708-8809727 Msa0235960:intron 35.0%
TTTGTATGATGAATTTGGGG+TGG + chr2_2:8809740-8809759 None:intergenic 35.0%
ATTCATCATACAAAGCTCTC+AGG - chr2_2:8809746-8809765 Msa0235960:intron 35.0%
!! CTTTGGAAAACCTGCAATTT+TGG + chr2_2:8809842-8809861 None:intergenic 35.0%
! CAAAATTGCAGGTTTTCCAA+AGG - chr2_2:8809840-8809859 Msa0235960:intron 35.0%
ATGGACAGAACAGTTTGTAT+AGG - chr2_2:8810045-8810064 Msa0235960:intron 35.0%
!!! CGCAATATTGCCGATTTTAA+AGG - chr2_2:8810345-8810364 Msa0235960:intron 35.0%
!! TTGCGATCCTCCTTTAAAAT+CGG + chr2_2:8810358-8810377 None:intergenic 35.0%
TAACCTATGCAATATTGCGT+AGG + chr2_2:8810512-8810531 None:intergenic 35.0%
ATCTTAAAATCATAGCCGTC+GGG + chr2_2:8810796-8810815 None:intergenic 35.0%
TGTTAGTAACTCAGATTGGT+TGG + chr2_2:8810882-8810901 None:intergenic 35.0%
ATGCTCGTGAATGGATAAAA+GGG - chr2_2:8811261-8811280 Msa0235960:CDS 35.0%
ATTTGTAGTGTTCATGGTCA+TGG - chr2_2:8811403-8811422 Msa0235960:CDS 35.0%
! TGCCTTCTTGTTCTTTTCTA+TGG - chr2_2:8811544-8811563 Msa0235960:CDS 35.0%
AGTAAATACCAAGATGGTTG+TGG + chr2_2:8811570-8811589 None:intergenic 35.0%
TGTTGCAGTAAATACCAAGA+TGG + chr2_2:8811576-8811595 None:intergenic 35.0%
TCATTGCAAAAATGCTGACA+GGG + chr2_2:8811661-8811680 None:intergenic 35.0%
ATCATTGCAAAAATGCTGAC+AGG + chr2_2:8811662-8811681 None:intergenic 35.0%
CAATCTTCTTAAGACTCCTA+AGG + chr2_2:8811730-8811749 None:intergenic 35.0%
!! TGTTCCTCAAGTTGATCAAA+AGG - chr2_2:8811748-8811767 Msa0235960:CDS 35.0%
!! TGGTGAGAAGCTTTGAAATG+CGG + chr2_2:8806846-8806865 None:intergenic 40.0%
TTCAAAGCTTCTCACCAAAC+TGG - chr2_2:8806849-8806868 Msa0235960:CDS 40.0%
TCAAAGCTTCTCACCAAACT+GGG - chr2_2:8806850-8806869 Msa0235960:CDS 40.0%
CTCGTCTCAGTTCTTCAAAT+CGG - chr2_2:8806878-8806897 Msa0235960:CDS 40.0%
GACGCTATCTTCAAAAAGCA+GGG + chr2_2:8806977-8806996 None:intergenic 40.0%
!! ATTTGATTTTGTAGTCCGGC+CGG - chr2_2:8807098-8807117 Msa0235960:CDS 40.0%
TCCAACAATTCATCAATCCC+CGG + chr2_2:8807165-8807184 None:intergenic 40.0%
! AGCGTTAGCGTTGAAATATG+AGG - chr2_2:8807185-8807204 Msa0235960:CDS 40.0%
! GTTAGCGTTGAAATATGAGG+TGG - chr2_2:8807188-8807207 Msa0235960:CDS 40.0%
ATTCGTGTAGGAATGTGGAA+GGG - chr2_2:8807301-8807320 Msa0235960:CDS 40.0%
!! TGTGAAGGATTTGGGTTTTG+TGG - chr2_2:8807329-8807348 Msa0235960:intron 40.0%
!! GTGAAGGATTTGGGTTTTGT+GGG - chr2_2:8807330-8807349 Msa0235960:intron 40.0%
TCGTGTTCACATCTGATCAA+AGG - chr2_2:8807558-8807577 Msa0235960:intron 40.0%
GGGCAATGAATGTAATGATG+TGG - chr2_2:8807731-8807750 Msa0235960:intron 40.0%
!! GTAATGATGTGGATTAGTGG+TGG - chr2_2:8807742-8807761 Msa0235960:intron 40.0%
GGCAATGCTTAACATGTGTA+TGG - chr2_2:8807882-8807901 Msa0235960:intron 40.0%
GATTTCTCAGTTCATGGACA+TGG - chr2_2:8808005-8808024 Msa0235960:intron 40.0%
TATTATGGTAGCACTTGCTC+TGG - chr2_2:8808063-8808082 Msa0235960:intron 40.0%
! AGAGAGTGTACTTAAGAGTG+GGG + chr2_2:8808157-8808176 None:intergenic 40.0%
! GAGAGAGTGTACTTAAGAGT+GGG + chr2_2:8808158-8808177 None:intergenic 40.0%
! AGAGAGAGTGTACTTAAGAG+TGG + chr2_2:8808159-8808178 None:intergenic 40.0%
ACTTACAAGGAGATTAGGAG+GGG + chr2_2:8808226-8808245 None:intergenic 40.0%
CAACTTACAAGGAGATTAGG+AGG + chr2_2:8808228-8808247 None:intergenic 40.0%
CGTAACAACGAATGAAAGGA+AGG - chr2_2:8808406-8808425 Msa0235960:CDS 40.0%
ACAACGAATGAAAGGAAGGA+AGG - chr2_2:8808410-8808429 Msa0235960:CDS 40.0%
TGACAGTTAGATCAGATCAC+AGG + chr2_2:8808452-8808471 None:intergenic 40.0%
TACAGCTGAGGACTAAATCT+TGG - chr2_2:8808506-8808525 Msa0235960:CDS 40.0%
CTAATTACACCACTAGTGGA+AGG + chr2_2:8808580-8808599 None:intergenic 40.0%
! GAAGAAAAATGAGGTTGGCT+AGG - chr2_2:8808985-8809004 Msa0235960:intron 40.0%
!! GGCTAGGTGTTGATTATGAA+CGG - chr2_2:8809001-8809020 Msa0235960:intron 40.0%
! TGTTGATTATGAACGGATGC+AGG - chr2_2:8809008-8809027 Msa0235960:intron 40.0%
!!! CTTGAATGCAGAAGGCATTT+TGG + chr2_2:8809117-8809136 None:intergenic 40.0%
GTTTGACTCTTGAATGCAGA+AGG + chr2_2:8809125-8809144 None:intergenic 40.0%
ATTCAAGAGTCAAACCCAGA+TGG - chr2_2:8809130-8809149 Msa0235960:intron 40.0%
TTCAAGAGTCAAACCCAGAT+GGG - chr2_2:8809131-8809150 Msa0235960:intron 40.0%
TAGGTCAAGGACACAAGTTA+AGG + chr2_2:8809377-8809396 None:intergenic 40.0%
AGATGTGGAAACTGGCAAAA+AGG - chr2_2:8809679-8809698 Msa0235960:intron 40.0%
GATGTGGAAACTGGCAAAAA+GGG - chr2_2:8809680-8809699 Msa0235960:intron 40.0%
TTGCTTATGGCAGCTTGTAA+TGG - chr2_2:8810132-8810151 Msa0235960:intron 40.0%
CAGACATGCTCAATTGATTG+AGG + chr2_2:8810195-8810214 None:intergenic 40.0%
TGAGCAGAACATACTGTACA+TGG - chr2_2:8810221-8810240 Msa0235960:intron 40.0%
AAAATGGTGAGACATCTTGC+TGG - chr2_2:8810291-8810310 Msa0235960:intron 40.0%
AATTGCAAGAGGAGTTGCAT+TGG - chr2_2:8810394-8810413 Msa0235960:intron 40.0%
!! AGGATGCTACAACTTGTTAG+TGG + chr2_2:8810492-8810511 None:intergenic 40.0%
CATCCTACGCAATATTGCAT+AGG - chr2_2:8810506-8810525 Msa0235960:intron 40.0%
!! TTTTCATTGGTGGTCCATGT+TGG - chr2_2:8810681-8810700 Msa0235960:intron 40.0%
TCATTAGAGAGACACCAACA+TGG + chr2_2:8810698-8810717 None:intergenic 40.0%
CAAGCAGATTACTGTGTGAA+TGG + chr2_2:8810756-8810775 None:intergenic 40.0%
!! CTGCTTGAATTTGTTGGATC+AGG - chr2_2:8810769-8810788 Msa0235960:intron 40.0%
! TTCTGAAAGATGCTCGTGAA+TGG - chr2_2:8811252-8811271 Msa0235960:CDS 40.0%
GATGCTCGTGAATGGATAAA+AGG - chr2_2:8811260-8811279 Msa0235960:CDS 40.0%
TGTAGCACTTGCAAGTTCAT+TGG - chr2_2:8811430-8811449 Msa0235960:CDS 40.0%
AAGGTGATGTGAGGATTGAA+AGG + chr2_2:8811472-8811491 None:intergenic 40.0%
CTAAAATCCCACAACTCACA+AGG + chr2_2:8811491-8811510 None:intergenic 40.0%
! CTTGTGAGTTGTGGGATTTT+AGG - chr2_2:8811489-8811508 Msa0235960:CDS 40.0%
!! ATTTTAGGAGGTCTCTACCT+AGG - chr2_2:8811504-8811523 Msa0235960:CDS 40.0%
GTCTCTACCTAGGAAACTAA+TGG - chr2_2:8811514-8811533 Msa0235960:CDS 40.0%
! AAACCTGCCATTAGTTTCCT+AGG + chr2_2:8811524-8811543 None:intergenic 40.0%
GGCCATAGAAAAGAACAAGA+AGG + chr2_2:8811549-8811568 None:intergenic 40.0%
TGCCATGAAAGGATGTCTTA+TGG - chr2_2:8811700-8811719 Msa0235960:CDS 40.0%
TGCCATAAGACATCCTTTCA+TGG + chr2_2:8811705-8811724 None:intergenic 40.0%
! TGCTCCTTTTGATCAACTTG+AGG + chr2_2:8811755-8811774 None:intergenic 40.0%
!! TGAGAAGCTTTGAAATGCGG+CGG + chr2_2:8806843-8806862 None:intergenic 45.0%
CGACGCTATCTTCAAAAAGC+AGG + chr2_2:8806978-8806997 None:intergenic 45.0%
TTCAAAAACTTCACCACCGC+CGG - chr2_2:8807071-8807090 Msa0235960:CDS 45.0%
AAATCAAATTTCTCCGGCGG+TGG + chr2_2:8807087-8807106 None:intergenic 45.0%
! ATCGGTTCACCATCAATCAC+CGG + chr2_2:8807120-8807139 None:intergenic 45.0%
TTTCTACGTGTTGAAGCGAC+CGG - chr2_2:8807143-8807162 Msa0235960:CDS 45.0%
ACCGGGGATTGATGAATTGT+TGG - chr2_2:8807161-8807180 Msa0235960:CDS 45.0%
! AGCGTTGAAATATGAGGTGG+TGG - chr2_2:8807191-8807210 Msa0235960:CDS 45.0%
! GCTTAAGGAGTATGCGAGTT+TGG - chr2_2:8807227-8807246 Msa0235960:CDS 45.0%
GATGCGAAATAAACCCGTTC+CGG + chr2_2:8807269-8807288 None:intergenic 45.0%
! GGAACGGGTTTATTTCGCAT+CGG - chr2_2:8807268-8807287 Msa0235960:CDS 45.0%
! GGCTTTATAGGGATTCGTGT+AGG - chr2_2:8807289-8807308 Msa0235960:CDS 45.0%
TAGGGATTCGTGTAGGAATG+TGG - chr2_2:8807296-8807315 Msa0235960:CDS 45.0%
GATTCGTGTAGGAATGTGGA+AGG - chr2_2:8807300-8807319 Msa0235960:CDS 45.0%
AGGGAAGCTTGTGAAGGATT+TGG - chr2_2:8807320-8807339 Msa0235960:intron 45.0%
GGGAAGCTTGTGAAGGATTT+GGG - chr2_2:8807321-8807340 Msa0235960:intron 45.0%
!! AGGATTTGGGTTTTGTGGGT+AGG - chr2_2:8807334-8807353 Msa0235960:intron 45.0%
!! GGATTTGGGTTTTGTGGGTA+GGG - chr2_2:8807335-8807354 Msa0235960:intron 45.0%
TGTGGGTAGGGATTTGAAGA+AGG - chr2_2:8807347-8807366 Msa0235960:intron 45.0%
ATGCTTGTGATCGTGAGCTT+TGG - chr2_2:8807448-8807467 Msa0235960:intron 45.0%
!! CGTGAGCTTTGGAAATTGAG+AGG - chr2_2:8807459-8807478 Msa0235960:intron 45.0%
TAACACCACCGTGTATGTGT+CGG - chr2_2:8807594-8807613 Msa0235960:intron 45.0%
!! ACAAAGCACCGACACATACA+CGG + chr2_2:8807605-8807624 None:intergenic 45.0%
CAATTATGCAGATTGCAGGG+TGG - chr2_2:8808114-8808133 Msa0235960:CDS 45.0%
! GAGAGTGTACTTAAGAGTGG+GGG + chr2_2:8808156-8808175 None:intergenic 45.0%
ATGACAGACTTGTACAGCTG+AGG - chr2_2:8808494-8808513 Msa0235960:CDS 45.0%
! CTAGTGATGATGAGCCATGT+AGG - chr2_2:8808614-8808633 Msa0235960:intron 45.0%
GCTGTCATTGGCCAATTCTT+TGG - chr2_2:8808792-8808811 Msa0235960:CDS 45.0%
! ATTTTCGAAGGCTCCCATCT+GGG + chr2_2:8809147-8809166 None:intergenic 45.0%
! AATTTTCGAAGGCTCCCATC+TGG + chr2_2:8809148-8809167 None:intergenic 45.0%
TGCTTCCGTTGAGTTTGATG+TGG + chr2_2:8809716-8809735 None:intergenic 45.0%
TTTAAAGGAGGATCGCAACC+TGG - chr2_2:8810360-8810379 Msa0235960:intron 45.0%
TTATGCCCTGCAATTGCAAG+AGG - chr2_2:8810383-8810402 Msa0235960:intron 45.0%
CAACTCCTCTTGCAATTGCA+GGG + chr2_2:8810391-8810410 None:intergenic 45.0%
GCGCTGTTAGTAACTCAGAT+TGG + chr2_2:8810886-8810905 None:intergenic 45.0%
AGAGTAATCTTGGTAAGCCG+CGG + chr2_2:8811373-8811392 None:intergenic 45.0%
!! TGCAAGTTCATTGGCTTCAG+TGG - chr2_2:8811439-8811458 Msa0235960:CDS 45.0%
ACAACTCACAAGGTGATGTG+AGG + chr2_2:8811481-8811500 None:intergenic 45.0%
TCACATCACCTTGTGAGTTG+TGG - chr2_2:8811480-8811499 Msa0235960:CDS 45.0%
CACATCACCTTGTGAGTTGT+GGG - chr2_2:8811481-8811500 Msa0235960:CDS 45.0%
! GTGAGTTGTGGGATTTTAGG+AGG - chr2_2:8811492-8811511 Msa0235960:CDS 45.0%
CTACCTAGGAAACTAATGGC+AGG - chr2_2:8811518-8811537 Msa0235960:CDS 45.0%
TTCTATGGCCACAACCATCT+TGG - chr2_2:8811559-8811578 Msa0235960:CDS 45.0%
GATGTCTTATGGCACTCCTT+AGG - chr2_2:8811711-8811730 Msa0235960:CDS 45.0%
AAGACATTAACCGCCGTCGT+TGG - chr2_2:8806755-8806774 Msa0235960:CDS 50.0%
!! GAGTTTTTCGACGCCAACGA+CGG + chr2_2:8806771-8806790 None:intergenic 50.0%
GCAGCGACGACGTTCTTTAT+CGG + chr2_2:8806793-8806812 None:intergenic 50.0%
GTGGATGGAAGAAAGAACGG+CGG + chr2_2:8806821-8806840 None:intergenic 50.0%
GCGGTGGATGGAAGAAAGAA+CGG + chr2_2:8806824-8806843 None:intergenic 50.0%
!! GGTGATTGATGGTGAACCGA+TGG - chr2_2:8807119-8807138 Msa0235960:CDS 50.0%
TTCTACGTGTTGAAGCGACC+GGG - chr2_2:8807144-8807163 Msa0235960:CDS 50.0%
!! TGAGGTGGTGGTTTTTACGG+CGG - chr2_2:8807203-8807222 Msa0235960:CDS 50.0%
GGAGTATGCGAGTTTGGTTG+TGG - chr2_2:8807233-8807252 Msa0235960:CDS 50.0%
ATGCGAGTTTGGTTGTGGAC+CGG - chr2_2:8807238-8807257 Msa0235960:CDS 50.0%
TGTGGAAGGGAAGCTTGTGA+AGG - chr2_2:8807314-8807333 Msa0235960:CDS 50.0%
! CATCTGATCAAAGGCGTGTC+TGG - chr2_2:8807567-8807586 Msa0235960:intron 50.0%
ACAAGGAGATTAGGAGGGGA+TGG + chr2_2:8808222-8808241 None:intergenic 50.0%
TGCAATTGCAGGGCATAACC+AGG + chr2_2:8810381-8810400 None:intergenic 50.0%
GCAACTCCTCTTGCAATTGC+AGG + chr2_2:8810392-8810411 None:intergenic 50.0%
TTTGTTGGATCAGGACCCGA+CGG - chr2_2:8810778-8810797 Msa0235960:intron 50.0%
CAAAGACAACTGTCGCGACA+AGG + chr2_2:8811316-8811335 None:intergenic 50.0%
TGTCGCGACAGTTGTCTTTG+CGG - chr2_2:8811316-8811335 Msa0235960:CDS 50.0%
!! TTTTTCGACGCCAACGACGG+CGG + chr2_2:8806768-8806787 None:intergenic 55.0%
GATGGAAGAAAGAACGGCGG+AGG + chr2_2:8806818-8806837 None:intergenic 55.0%
!! CTTTGAAATGCGGCGGTGGA+TGG + chr2_2:8806836-8806855 None:intergenic 55.0%
!! GAAGCTTTGAAATGCGGCGG+TGG + chr2_2:8806840-8806859 None:intergenic 55.0%
GAGACGAGTGAGTCCCAGTT+TGG + chr2_2:8806866-8806885 None:intergenic 55.0%
GTTCACCATCAATCACCGGC+CGG + chr2_2:8807116-8807135 None:intergenic 55.0%
TCTACGTGTTGAAGCGACCG+GGG - chr2_2:8807145-8807164 Msa0235960:CDS 55.0%
! GGTTTTTACGGCGGCGCTTA+AGG - chr2_2:8807212-8807231 Msa0235960:CDS 55.0%
GAGTTTGGTTGTGGACCGGT+TGG - chr2_2:8807242-8807261 Msa0235960:CDS 55.0%
TAAACCCGTTCCGGTCCAAC+CGG + chr2_2:8807260-8807279 None:intergenic 55.0%
!! AAGCACCGACACATACACGG+TGG + chr2_2:8807602-8807621 None:intergenic 55.0%
GTAGTCCGGCCGGTGATTGA+TGG - chr2_2:8807108-8807127 Msa0235960:CDS 60.0%
TGGTTGTGGACCGGTTGGAC+CGG - chr2_2:8807247-8807266 Msa0235960:CDS 60.0%
GCAGCTTACACTGTCCCAGG+AGG - chr2_2:8811338-8811357 Msa0235960:CDS 60.0%
GTGGACCGGTTGGACCGGAA+CGG - chr2_2:8807252-8807271 Msa0235960:CDS 65.0%
TGGACCGGTTGGACCGGAAC+GGG - chr2_2:8807253-8807272 Msa0235960:CDS 65.0%
GCGGCAGCTTACACTGTCCC+AGG - chr2_2:8811335-8811354 Msa0235960:CDS 65.0%
CGCGGTCGTTTGTGCCTCCT+GGG + chr2_2:8811355-8811374 None:intergenic 65.0%
! CCAGGAGGCACAAACGACCG+CGG - chr2_2:8811353-8811372 Msa0235960:CDS 65.0%
CCGCGGTCGTTTGTGCCTCC+TGG + chr2_2:8811356-8811375 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 8806712 8811784 8806712 ID=Msa0235960;Name=Msa0235960
chr2_2 mRNA 8806712 8811784 8806712 ID=Msa0235960-mRNA-1;Parent=Msa0235960;Name=Msa0235960-mRNA-1;_AED=0.07;_eAED=0.08;_QI=0|0.2|0|0.83|1|1|6|0|761
chr2_2 exon 8810908 8811784 8810908 ID=Msa0235960-mRNA-1:exon:3833;Parent=Msa0235960-mRNA-1
chr2_2 exon 8809317 8809466 8809317 ID=Msa0235960-mRNA-1:exon:3832;Parent=Msa0235960-mRNA-1
chr2_2 exon 8808729 8808881 8808729 ID=Msa0235960-mRNA-1:exon:3831;Parent=Msa0235960-mRNA-1
chr2_2 exon 8808635 8808645 8808635 ID=Msa0235960-mRNA-1:exon:3830;Parent=Msa0235960-mRNA-1
chr2_2 exon 8808074 8808541 8808074 ID=Msa0235960-mRNA-1:exon:3829;Parent=Msa0235960-mRNA-1
chr2_2 exon 8806712 8807338 8806712 ID=Msa0235960-mRNA-1:exon:3828;Parent=Msa0235960-mRNA-1
chr2_2 CDS 8810908 8811784 8810908 ID=Msa0235960-mRNA-1:cds;Parent=Msa0235960-mRNA-1
chr2_2 CDS 8809317 8809466 8809317 ID=Msa0235960-mRNA-1:cds;Parent=Msa0235960-mRNA-1
chr2_2 CDS 8808729 8808881 8808729 ID=Msa0235960-mRNA-1:cds;Parent=Msa0235960-mRNA-1
chr2_2 CDS 8808635 8808645 8808635 ID=Msa0235960-mRNA-1:cds;Parent=Msa0235960-mRNA-1
chr2_2 CDS 8808074 8808541 8808074 ID=Msa0235960-mRNA-1:cds;Parent=Msa0235960-mRNA-1
chr2_2 CDS 8806712 8807338 8806712 ID=Msa0235960-mRNA-1:cds;Parent=Msa0235960-mRNA-1
Gene Sequence

>Msa0235960

ATGGTTTCAAAGACGAAGAAGAAAGCATCAACCAAATCAATCAAAGACATTAACCGCCGTCGTTGGCGTCGAAAAACTCCGATAAAGAACGTCGTCGCTGCTTCCTCCGCCGTTCTTTCTTCCATCCACCGCCGCATTTCAAAGCTTCTCACCAAACTGGGACTCACTCGTCTCAGTTCTTCAAATCGGAAACGAAACAGCTACAAAATCCTCAAGAAATCAACACATTTCAATTTTCAACAGCAAGACACCGTTTGCAAAACCCTGCTTTTTGAAGATAGCGTCGAAAATTCTCTTCTTCCACCTTCAATATCAAATCGGAAAACCGTTTTTCTTGATTTAGATGAAACTTTGGTTCATTCAAAAACTTCACCACCGCCGGAGAAATTTGATTTTGTAGTCCGGCCGGTGATTGATGGTGAACCGATGGATTTCTACGTGTTGAAGCGACCGGGGATTGATGAATTGTTGGAAGCGTTAGCGTTGAAATATGAGGTGGTGGTTTTTACGGCGGCGCTTAAGGAGTATGCGAGTTTGGTTGTGGACCGGTTGGACCGGAACGGGTTTATTTCGCATCGGCTTTATAGGGATTCGTGTAGGAATGTGGAAGGGAAGCTTGTGAAGGATTTGGGTTTTGTGGGTAGGGATTTGAAGAAGGTTGTTATTGTGGATGATAATCCAGTTTCATTTTCGAATCAACCTGAGAATGCGATTTTGATTAAGCCTTTTGTTGATGATGCTTGTGATCGTGAGCTTTGGAAATTGAGAGGCTTTTTTGATGGGTGTGATTGTTTTGATGATATGAGGGATGCTGTTAAACACTATGCTGCTGAGAAAGAACGTCGCTCGTGTTCACATCTGATCAAAGGCGTGTCTGGCACAGCTTTATTTTTATTGAAAGGGTACGGCGAACTCGCTGATCAAAAGAAAGAAAATGATTTGACAGCTCTACAATTGCTAGCCAAAATGCCTTCTGCATTCAAGAGTCAAACCCAGATGGGAGCCTTCGAAAATTTCATATATCCTCTGCTTCCTGATTATCAAGATGATGCATATTACCTTCAAGATAATGATATTACTAGAAAAAGAGAAGATGTGGAAACTGGCAAAAAGGGCAAAAATGAACCACATCAAACTCAACGGAAGCAACCACCCCAAATTCATCATACAAAGCTCTCAGGTTTTCCAAAGAATGGAAGAGTATTAAAAAACTTTGGAAGAAAAAAAATGCATAACTTGTTAGCAAAGAATGACAATTCATGGAAACTTAGTTCAATAGCAATGGACAGAACAGTTTGTATAGGAATACAAAATGAGAATCAAGAAGAACAAGATGTTTCAAGCCACAAAGCTATTACTTATACACTGTTGCTTATGGCAGCTTGTAATGGAATCATAGAGATAGTAGAATTGGTAATTCGTTTTCATCCTCAATCAATTGAGCATGTCTGTGAAGATGAGCAGAACATACTGTACATGGCTGTGAAACATCACCAGTTAGAAATTTTCCGAATGTTGAAAAAACGGAAAATGGTGAGACATCTTGCTGGAAAAATAGACAACAAAAATAACACTGTTCTCCGCAATATTGCCGATTTTAAAGGAGGATCGCAACCTGGTTATGCCCTGCAATTGCAAGAGGAGTTGCATTGGTTTGAACGCATAGAGAAGAAACTTCCTTATCATTACATCATTCACAAAAACGACAACAACCAAACTGCAAGGGAACTCTTCGAGCAAAAGCACAAACAACTTCTGAAAGATGCTCGTGAATGGATAAAAGGGACAGCTCAGTCATGTTCTGCTGTAGCTGTCCTTGTCGCGACAGTTGTCTTTGCGGCAGCTTACACTGTCCCAGGAGGCACAAACGACCGCGGCTTACCAAGATTACTCTATCATCCTATATTTGTAGTGTTCATGGTCATGGATGTTGTAGCACTTGCAAGTTCATTGGCTTCAGTGGTTATGTTCCTTTCAATCCTCACATCACCTTGTGAGTTGTGGGATTTTAGGAGGTCTCTACCTAGGAAACTAATGGCAGGTTTTGCCTTCTTGTTCTTTTCTATGGCCACAACCATCTTGGTATTTACTGCAACAATTTTGGTGAACATCAAGTTAGATAAGAGCAAATGGAATTCAAGTTATAGTGCAGCATTTTTCCCTGTCAGCATTTTTGCAATGATGCAGTTCACTTTATATGTTGCCATGAAAGGATGTCTTATGGCACTCCTTAGGAGTCTTAAGAAGATTGTTCCTCAAGTTGATCAAAAGGAGCAAGAAAAATAG
Protein sequence

>Msa0235960

MVSKTKKKASTKSIKDINRRRWRRKTPIKNVVAASSAVLSSIHRRISKLLTKLGLTRLSSSNRKRNSYKILKKSTHFNFQQQDTVCKTLLFEDSVENSLLPPSISNRKTVFLDLDETLVHSKTSPPPEKFDFVVRPVIDGEPMDFYVLKRPGIDELLEALALKYEVVVFTAALKEYASLVVDRLDRNGFISHRLYRDSCRNVEGKLVKDLGFVGRDLKKVVIVDDNPVSFSNQPENAILIKPFVDDACDRELWKLRGFFDGCDCFDDMRDAVKHYAAEKERRSCSHLIKGVSGTALFLLKGYGELADQKKENDLTALQLLAKMPSAFKSQTQMGAFENFIYPLLPDYQDDAYYLQDNDITRKREDVETGKKGKNEPHQTQRKQPPQIHHTKLSGFPKNGRVLKNFGRKKMHNLLAKNDNSWKLSSIAMDRTVCIGIQNENQEEQDVSSHKAITYTLLLMAACNGIIEIVELVIRFHPQSIEHVCEDEQNILYMAVKHHQLEIFRMLKKRKMVRHLAGKIDNKNNTVLRNIADFKGGSQPGYALQLQEELHWFERIEKKLPYHYIIHKNDNNQTARELFEQKHKQLLKDAREWIKGTAQSCSAVAVLVATVVFAAAYTVPGGTNDRGLPRLLYHPIFVVFMVMDVVALASSLASVVMFLSILTSPCELWDFRRSLPRKLMAGFAFLFFSMATTILVFTATILVNIKLDKSKWNSSYSAAFFPVSIFAMMQFTLYVAMKGCLMALLRSLKKIVPQVDQKEQEK*