Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0275770 | XP_013462789.1 | 94.495 | 109 | 6 | 0 | 1 | 109 | 1 | 109 | 3.68e-72 | 220 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0025160 | Msa0275770 | 0.811949 | 5.420108e-51 | -8.615850e-47 |
| Msa0050790 | Msa0275770 | 0.805307 | 1.422209e-49 | -8.615850e-47 |
| Msa0054690 | Msa0275770 | 0.828752 | 7.550523e-55 | -8.615850e-47 |
| Msa0081520 | Msa0275770 | 0.810670 | 1.026884e-50 | -8.615850e-47 |
| Msa0158860 | Msa0275770 | 0.815661 | 8.246902e-52 | -8.615850e-47 |
| Msa0173540 | Msa0275770 | 0.800602 | 1.334168e-48 | -8.615850e-47 |
| Msa0174450 | Msa0275770 | 0.806259 | 8.971752e-50 | -8.615850e-47 |
| Msa0275770 | Msa0275820 | 0.956469 | 2.740121e-114 | -8.615850e-47 |
| Msa0275770 | Msa0304570 | 0.801999 | 6.905777e-49 | -8.615850e-47 |
| Msa0275770 | Msa0304680 | 0.809244 | 2.082510e-50 | -8.615850e-47 |
| Msa0275770 | Msa0307270 | 0.807144 | 5.834832e-50 | -8.615850e-47 |
| Msa0275770 | Msa0364120 | 0.801020 | 1.096385e-48 | -8.615850e-47 |
| Msa0275770 | Msa0369790 | 0.828153 | 1.052948e-54 | -8.615850e-47 |
| Msa0275770 | Msa0375640 | 0.824351 | 8.459637e-54 | -8.615850e-47 |
| Msa0275770 | Msa0378720 | 0.801090 | 1.061047e-48 | -8.615850e-47 |
| Msa0275770 | Msa0424140 | 0.817187 | 3.755266e-52 | -8.615850e-47 |
| Msa0275770 | Msa0424160 | 0.833002 | 6.848148e-56 | -8.615850e-47 |
| Msa0275770 | Msa0428370 | 0.801480 | 8.828634e-49 | -8.615850e-47 |
| Msa0275770 | Msa0430260 | 0.809411 | 1.917697e-50 | -8.615850e-47 |
| Msa0275770 | Msa0453040 | 0.843792 | 1.126552e-58 | -8.615850e-47 |
| Msa0275770 | Msa0464000 | 0.826258 | 2.995014e-54 | -8.615850e-47 |
| Msa0275770 | Msa0472950 | 0.822694 | 2.064906e-53 | -8.615850e-47 |
| Msa0275770 | Msa0474730 | 0.810304 | 1.232037e-50 | -8.615850e-47 |
| Msa0275770 | Msa0500380 | 0.802007 | 6.881020e-49 | -8.615850e-47 |
| Msa0275770 | Msa0509550 | 0.834439 | 2.996239e-56 | -8.615850e-47 |
| Msa0275770 | Msa0515800 | 0.826248 | 3.011274e-54 | -8.615850e-47 |
| Msa0275770 | Msa0550050 | 0.810501 | 1.117252e-50 | -8.615850e-47 |
| Msa0275770 | Msa0557460 | 0.804301 | 2.306560e-49 | -8.615850e-47 |
| Msa0275770 | Msa0567170 | 0.802415 | 5.670453e-49 | -8.615850e-47 |
| Msa0275770 | Msa0580370 | 0.814478 | 1.509902e-51 | -8.615850e-47 |
| Msa0275770 | Msa0591170 | 0.815945 | 7.127061e-52 | -8.615850e-47 |
| Msa0275770 | Msa0605560 | 0.814927 | 1.200474e-51 | -8.615850e-47 |
| Msa0275770 | Msa0626450 | 0.814047 | 1.879167e-51 | -8.615850e-47 |
| Msa0275770 | Msa0626490 | 0.809554 | 1.786893e-50 | -8.615850e-47 |
| Msa0275770 | Msa0658640 | 0.815018 | 1.145805e-51 | -8.615850e-47 |
| Msa0275770 | Msa0661150 | 0.817559 | 3.097174e-52 | -8.615850e-47 |
| Msa0275770 | Msa0664600 | 0.806412 | 8.329625e-50 | -8.615850e-47 |
| Msa0275770 | Msa0715060 | 0.808632 | 2.815208e-50 | -8.615850e-47 |
| Msa0275770 | Msa0738700 | 0.805048 | 1.611112e-49 | -8.615850e-47 |
| Msa0275770 | Msa0768990 | 0.820134 | 8.053285e-53 | -8.615850e-47 |
| Msa0275770 | Msa0783530 | 0.817290 | 3.560717e-52 | -8.615850e-47 |
| Msa0275770 | Msa0795870 | 0.805465 | 1.317872e-49 | -8.615850e-47 |
| Msa0275770 | Msa0819170 | 0.814637 | 1.392189e-51 | -8.615850e-47 |
| Msa0275770 | Msa0833650 | 0.807531 | 4.831221e-50 | -8.615850e-47 |
| Msa0275770 | Msa0855830 | 0.802922 | 4.456966e-49 | -8.615850e-47 |
| Msa0275770 | Msa0878280 | 0.814355 | 1.607360e-51 | -8.615850e-47 |
| Msa0275770 | Msa1042160 | 0.840676 | 7.532857e-58 | -8.615850e-47 |
| Msa0275770 | Msa1042190 | 0.838814 | 2.299828e-57 | -8.615850e-47 |
| Msa0275770 | Msa1074220 | 0.806365 | 8.520781e-50 | -8.615850e-47 |
| Msa0275770 | Msa1080000 | 0.800531 | 1.379600e-48 | -8.615850e-47 |
| Msa0275770 | Msa1132840 | 0.811803 | 5.831651e-51 | -8.615850e-47 |
| Msa0275770 | Msa1145580 | 0.801511 | 8.697235e-49 | -8.615850e-47 |
| Msa0275770 | Msa1160920 | 0.805262 | 1.453406e-49 | -8.615850e-47 |
| Msa0275770 | Msa1167470 | 0.828194 | 1.029513e-54 | -8.615850e-47 |
| Msa0275770 | Msa1226520 | 0.814092 | 1.837182e-51 | -8.615850e-47 |
| Msa0275770 | Msa1268240 | 0.801788 | 7.632860e-49 | -8.615850e-47 |
| Msa0275770 | Msa1316330 | 0.806865 | 6.683856e-50 | -8.615850e-47 |
| Msa0275770 | Msa1359690 | 0.822028 | 2.948354e-53 | -8.615850e-47 |
| Msa0275770 | Msa1393780 | 0.803368 | 3.604469e-49 | -8.615850e-47 |
| Msa0275770 | Msa1409410 | 0.801776 | 7.674796e-49 | -8.615850e-47 |
| Msa0275770 | Msa1414170 | 0.819419 | 1.173266e-52 | -8.615850e-47 |
| Msa0275770 | Msa1437940 | 0.807635 | 4.591392e-50 | -8.615850e-47 |
| Msa0275770 | Msa1440630 | 0.803562 | 3.285052e-49 | -8.615850e-47 |
| Msa0275770 | Msa1440960 | 0.812128 | 4.955472e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0275770 | MtrunA17_Chr2g0288131 | 94.495 | 109 | 6 | 0 | 1 | 109 | 1 | 109 | 3.39e-76 | 220 |
| Msa0275770 | MtrunA17_Chr4g0074631 | 63.208 | 106 | 28 | 6 | 3 | 105 | 7 | 104 | 4.15e-32 | 108 |
| Msa0275770 | MtrunA17_Chr5g0443671 | 30.556 | 108 | 72 | 3 | 4 | 109 | 69 | 175 | 2.43e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0275770 | AT4G21780.1 | 44.643 | 112 | 49 | 5 | 1 | 103 | 1 | 108 | 9.69e-24 | 88.2 |
| Msa0275770 | AT4G21780.3 | 44.643 | 112 | 49 | 5 | 1 | 103 | 1 | 108 | 9.69e-24 | 88.2 |
| Msa0275770 | AT4G21780.2 | 44.643 | 112 | 49 | 5 | 1 | 103 | 1 | 108 | 9.69e-24 | 88.2 |
Find 19 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGCAAAGAAGCCTAGTTTC+TGG | 0.300098 | 2_3:-7922299 | Msa0275770:CDS |
| AATCAATTCAACAGAATTCC+TGG | 0.354806 | 2_3:-7922180 | Msa0275770:CDS |
| ATAGGCACAAGGGGCACTAT+TGG | 0.371860 | 2_3:-7922420 | Msa0275770:CDS |
| TGCCAACAACTTGGAAGAAA+AGG | 0.387622 | 2_3:-7922272 | Msa0275770:CDS |
| CCCCTTGTGCCTATAGCAAC+AGG | 0.417302 | 2_3:+7922429 | None:intergenic |
| TTGCTTTCTCCATGTCTACA+AGG | 0.428108 | 2_3:+7922316 | None:intergenic |
| AGTTTGAGTTAGATAGAAGA+GGG | 0.441913 | 2_3:-7922362 | Msa0275770:CDS |
| AGCAAAGAAGCACAGCAAAC+AGG | 0.456615 | 2_3:-7922248 | Msa0275770:CDS |
| ATGGCTGCACCTGTTGCTAT+AGG | 0.463713 | 2_3:-7922438 | Msa0275770:CDS |
| TATTGGATCACTAGTGAGAA+AGG | 0.511881 | 2_3:-7922403 | Msa0275770:CDS |
| CACCTGTTGCTATAGGCACA+AGG | 0.517230 | 2_3:-7922431 | Msa0275770:CDS |
| AGGATCTTGTAGCTGTAACC+AGG | 0.558959 | 2_3:+7922162 | None:intergenic |
| AAGTTTGAGTTAGATAGAAG+AGG | 0.566161 | 2_3:-7922363 | Msa0275770:CDS |
| ATGTCTACAAGGTGTTGCTG+AGG | 0.586253 | 2_3:+7922327 | None:intergenic |
| CCTGTTGCTATAGGCACAAG+GGG | 0.608011 | 2_3:-7922429 | Msa0275770:CDS |
| AGTTGTTGATATCATCCTTG+AGG | 0.616088 | 2_3:+7922142 | None:intergenic |
| TCAGCAACACCTTGTAGACA+TGG | 0.626160 | 2_3:-7922325 | Msa0275770:CDS |
| ACCTGTTGCTATAGGCACAA+GGG | 0.631732 | 2_3:-7922430 | Msa0275770:CDS |
| TTACAGCTACAAGATCCTCA+AGG | 0.653866 | 2_3:-7922157 | Msa0275770:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGTTTGAGTTAGATAGAAG+AGG | - | chr2_3:7922206-7922225 | Msa0275770:CDS | 30.0% |
| ! | AGTTTGAGTTAGATAGAAGA+GGG | - | chr2_3:7922207-7922226 | Msa0275770:CDS | 30.0% |
| ! | CTGTAACAGAACAGATTTTT+CGG | + | chr2_3:7922352-7922371 | None:intergenic | 30.0% |
| AATCAATTCAACAGAATTCC+TGG | - | chr2_3:7922389-7922408 | Msa0275770:CDS | 30.0% | |
| TATTGGATCACTAGTGAGAA+AGG | - | chr2_3:7922166-7922185 | Msa0275770:CDS | 35.0% | |
| ! | TTCCTTTTCTTCCAAGTTGT+TGG | + | chr2_3:7922302-7922321 | None:intergenic | 35.0% |
| AGTTGTTGATATCATCCTTG+AGG | + | chr2_3:7922430-7922449 | None:intergenic | 35.0% | |
| TTGCTTTCTCCATGTCTACA+AGG | + | chr2_3:7922256-7922275 | None:intergenic | 40.0% | |
| AAGCAAAGAAGCCTAGTTTC+TGG | - | chr2_3:7922270-7922289 | Msa0275770:CDS | 40.0% | |
| GTTGGCAAAAACCAGAAACT+AGG | + | chr2_3:7922284-7922303 | None:intergenic | 40.0% | |
| !!! | TCTGGTTTTTGCCAACAACT+TGG | - | chr2_3:7922288-7922307 | Msa0275770:CDS | 40.0% |
| TGCCAACAACTTGGAAGAAA+AGG | - | chr2_3:7922297-7922316 | Msa0275770:CDS | 40.0% | |
| TTACAGCTACAAGATCCTCA+AGG | - | chr2_3:7922412-7922431 | Msa0275770:CDS | 40.0% | |
| ACCTGTTGCTATAGGCACAA+GGG | - | chr2_3:7922139-7922158 | Msa0275770:CDS | 45.0% | |
| !! | ATGTCTACAAGGTGTTGCTG+AGG | + | chr2_3:7922245-7922264 | None:intergenic | 45.0% |
| TCAGCAACACCTTGTAGACA+TGG | - | chr2_3:7922244-7922263 | Msa0275770:CDS | 45.0% | |
| ! | AGCAAAGAAGCACAGCAAAC+AGG | - | chr2_3:7922321-7922340 | Msa0275770:CDS | 45.0% |
| AGGATCTTGTAGCTGTAACC+AGG | + | chr2_3:7922410-7922429 | None:intergenic | 45.0% | |
| ATGGCTGCACCTGTTGCTAT+AGG | - | chr2_3:7922131-7922150 | Msa0275770:CDS | 50.0% | |
| CACCTGTTGCTATAGGCACA+AGG | - | chr2_3:7922138-7922157 | Msa0275770:CDS | 50.0% | |
| CCTGTTGCTATAGGCACAAG+GGG | - | chr2_3:7922140-7922159 | Msa0275770:CDS | 50.0% | |
| !! | ATAGGCACAAGGGGCACTAT+TGG | - | chr2_3:7922149-7922168 | Msa0275770:CDS | 50.0% |
| CCCCTTGTGCCTATAGCAAC+AGG | + | chr2_3:7922143-7922162 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_3 | gene | 7922131 | 7922460 | 7922131 | ID=Msa0275770;Name=Msa0275770 |
| chr2_3 | mRNA | 7922131 | 7922460 | 7922131 | ID=Msa0275770-mRNA-1;Parent=Msa0275770;Name=Msa0275770-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|109 |
| chr2_3 | exon | 7922131 | 7922460 | 7922131 | ID=Msa0275770-mRNA-1:exon:3636;Parent=Msa0275770-mRNA-1 |
| chr2_3 | CDS | 7922131 | 7922460 | 7922131 | ID=Msa0275770-mRNA-1:cds;Parent=Msa0275770-mRNA-1 |
| Gene Sequence |
| Protein sequence |
