AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0158860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0158860 MtrunA17_Chr1g0171171 97.867 375 8 0 1 375 1 375 0.0 772
Msa0158860 MtrunA17_Chr1g0210771 75.069 361 85 3 19 375 23 382 0.0 580
Msa0158860 MtrunA17_Chr5g0430371 39.316 351 190 5 45 375 52 399 7.89e-97 294
Msa0158860 MtrunA17_Chr2g0283581 42.246 374 188 9 9 375 6 358 1.56e-95 289
Msa0158860 MtrunA17_Chr2g0325481 42.560 336 173 7 47 375 44 366 3.60e-93 283
Msa0158860 MtrunA17_Chr7g0258851 41.449 345 185 6 48 375 243 587 5.33e-93 290
Msa0158860 MtrunA17_Chr2g0314801 41.791 335 177 5 49 375 29 353 2.41e-91 278
Msa0158860 MtrunA17_Chr1g0175671 40.870 345 187 6 48 375 165 509 3.72e-91 283
Msa0158860 MtrunA17_Chr3g0097891 39.572 374 192 8 10 375 3 350 4.57e-91 277
Msa0158860 MtrunA17_Chr2g0314791 43.413 334 161 9 53 375 29 345 1.95e-89 273
Msa0158860 MtrunA17_Chr3g0105301 38.988 336 190 5 53 375 43 376 3.28e-88 271
Msa0158860 MtrunA17_Chr4g0041011 38.933 375 196 10 14 375 9 363 1.55e-87 269
Msa0158860 MtrunA17_Chr4g0045941 39.521 334 185 5 48 375 28 350 7.77e-87 266
Msa0158860 MtrunA17_Chr5g0413851 38.158 380 202 10 8 375 1 359 8.24e-86 264
Msa0158860 MtrunA17_Chr4g0071391 34.908 381 221 6 21 375 67 446 4.49e-85 265
Msa0158860 MtrunA17_Chr1g0213281 35.770 383 222 7 17 375 133 515 1.61e-84 266
Msa0158860 MtrunA17_Chr4g0045951 40.000 335 184 5 47 375 39 362 2.26e-84 260
Msa0158860 MtrunA17_Chr4g0006991 39.244 344 190 7 51 375 84 427 2.27e-84 263
Msa0158860 MtrunA17_Chr3g0085081 39.883 341 183 6 40 373 21 346 2.33e-84 260
Msa0158860 MtrunA17_Chr5g0413271 38.024 334 198 4 51 375 143 476 1.09e-83 263
Msa0158860 MtrunA17_Chr7g0269991 37.425 334 187 6 53 371 137 463 1.84e-83 261
Msa0158860 MtrunA17_Chr3g0091031 39.589 341 184 6 40 373 21 346 2.59e-82 254
Msa0158860 MtrunA17_Chr4g0068111 37.135 342 194 6 53 375 114 453 1.96e-81 256
Msa0158860 MtrunA17_Chr8g0360521 39.942 343 182 8 53 375 71 409 3.50e-81 254
Msa0158860 MtrunA17_Chr4g0005491 37.037 351 198 9 48 375 57 407 1.36e-80 252
Msa0158860 MtrunA17_Chr3g0085071 38.554 332 185 5 49 373 42 361 1.80e-80 251
Msa0158860 MtrunA17_Chr7g0243301 34.336 399 226 11 10 375 14 409 2.63e-77 244
Msa0158860 MtrunA17_Chr7g0242701 38.841 345 192 7 50 375 83 427 2.34e-76 242
Msa0158860 MtrunA17_Chr3g0113671 37.353 340 192 7 53 371 106 445 4.46e-76 242
Msa0158860 MtrunA17_Chr8g0368461 36.544 353 194 7 52 375 177 528 6.97e-76 244
Msa0158860 MtrunA17_Chr5g0437291 37.391 345 192 8 51 371 163 507 3.98e-73 236
Msa0158860 MtrunA17_Chr2g0287901 35.795 352 196 7 48 375 92 437 4.11e-73 234
Msa0158860 MtrunA17_Chr3g0114101 35.673 342 201 7 53 375 89 430 1.07e-71 230
Msa0158860 MtrunA17_Chr5g0437601 33.417 398 235 10 8 375 27 424 2.02e-71 229
Msa0158860 MtrunA17_Chr7g0259711 35.311 354 206 6 43 375 81 432 1.03e-70 228
Msa0158860 MtrunA17_Chr4g0005481 35.244 349 206 8 45 375 100 446 1.38e-70 228
Msa0158860 MtrunA17_Chr8g0384151 32.853 347 207 7 44 375 81 416 1.47e-62 206
Msa0158860 MtrunA17_Chr3g0126021 35.072 345 196 7 49 375 125 459 3.66e-62 206
Msa0158860 MtrunA17_Chr1g0178081 32.832 399 186 13 1 375 1 341 1.09e-57 191
Msa0158860 MtrunA17_Chr1g0209921 39.485 233 129 7 50 276 41 267 5.85e-55 181
Msa0158860 MtrunA17_Chr8g0347321 28.108 370 231 11 36 375 75 439 6.33e-43 155
Msa0158860 MtrunA17_Chr6g0476041 31.383 376 203 16 40 375 154 514 3.90e-41 151
Msa0158860 MtrunA17_Chr8g0384421 30.295 373 216 14 35 375 112 472 9.42e-40 147
Msa0158860 MtrunA17_Chr1g0188191 30.769 377 209 16 36 375 220 581 6.70e-39 146
Msa0158860 MtrunA17_Chr7g0226991 27.920 351 226 7 50 375 54 402 3.43e-38 141
Msa0158860 MtrunA17_Chr5g0414051 28.169 355 214 10 53 375 75 420 2.96e-37 139
Msa0158860 MtrunA17_Chr4g0049421 27.762 353 217 12 53 375 48 392 3.65e-37 138
Msa0158860 MtrunA17_Chr4g0070341 30.919 359 209 16 45 375 124 471 1.01e-36 138
Msa0158860 MtrunA17_Chr8g0391481 28.056 360 220 11 47 375 21 372 2.98e-36 135
Msa0158860 MtrunA17_Chr3g0140371 27.049 366 227 9 40 375 5 360 6.47e-36 134
Msa0158860 MtrunA17_Chr5g0414061 28.726 369 211 15 44 375 85 438 9.07e-35 132
Msa0158860 MtrunA17_Chr8g0387921 26.027 365 215 12 50 375 71 419 2.90e-34 131
Msa0158860 MtrunA17_Chr5g0414091 28.852 357 220 12 48 375 80 431 2.05e-33 129
Msa0158860 MtrunA17_Chr6g0456681 28.492 358 224 10 45 375 56 408 1.64e-32 126
Msa0158860 MtrunA17_Chr6g0456691 26.214 412 252 12 4 375 20 419 4.79e-32 125
Msa0158860 MtrunA17_Chr2g0283851 28.177 362 197 13 57 375 59 400 1.17e-31 123
Msa0158860 MtrunA17_Chr6g0455901 28.293 410 234 18 4 371 21 412 6.10e-31 122
Msa0158860 MtrunA17_Chr5g0414041 30.055 366 212 13 44 375 78 433 2.06e-29 118
Msa0158860 MtrunA17_Chr6g0451321 28.142 366 206 15 48 375 111 457 8.56e-29 116
Msa0158860 MtrunA17_Chr7g0221291 44.792 96 52 1 49 143 75 170 1.42e-22 93.6
Msa0158860 MtrunA17_Chr7g0221271 35.616 146 86 4 38 178 63 205 5.96e-22 92.8
Msa0158860 MtrunA17_Chr3g0085101 37.037 108 62 1 238 339 1 108 6.78e-18 79.0
Msa0158860 MtrunA17_Chr2g0287931 32.773 119 68 2 112 218 2 120 2.10e-15 72.4
Msa0158860 MtrunA17_Chr1g0209931 50.704 71 27 1 305 375 30 92 3.78e-15 70.9
Msa0158860 MtrunA17_Chr5g0429191 50.000 44 22 0 325 368 2 45 3.17e-11 58.2
Msa0158860 MtrunA17_Chr2g0287911 30.702 114 62 2 5 107 2 109 3.53e-11 59.7
Msa0158860 MtrunA17_Chr7g0221281 25.000 244 149 10 158 375 1 236 3.93e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0158860 AT3G54260.1 61.842 380 134 5 1 375 1 374 1.01e-169 479
Msa0158860 AT2G31110.2 39.623 371 205 6 13 375 3 362 9.47e-93 282
Msa0158860 AT5G06700.1 40.870 345 187 6 48 375 250 594 1.65e-90 284
Msa0158860 AT5G58600.1 39.130 345 193 6 44 375 60 400 6.46e-90 276
Msa0158860 AT1G29050.1 39.714 350 185 5 41 375 40 378 7.17e-90 275
Msa0158860 AT2G30900.1 41.369 336 175 8 51 375 41 365 3.89e-88 271
Msa0158860 AT2G34070.1 38.576 337 189 5 46 375 58 383 1.30e-87 270
Msa0158860 AT2G42570.1 39.940 333 183 5 49 375 43 364 3.43e-87 268
Msa0158860 AT3G12060.1 39.710 345 191 5 48 375 191 535 3.83e-87 274
Msa0158860 AT5G20590.1 38.551 345 194 6 48 375 136 479 5.65e-85 266
Msa0158860 AT5G01360.2 38.728 346 190 8 51 375 34 378 1.48e-84 262
Msa0158860 AT5G01360.1 38.728 346 190 8 51 375 90 434 5.46e-84 262
Msa0158860 AT5G49340.1 38.122 362 203 8 35 375 80 441 1.56e-83 262
Msa0158860 AT3G62390.1 36.000 375 205 8 23 371 102 467 2.97e-83 261
Msa0158860 AT2G30010.1 37.568 370 198 8 35 375 31 396 1.88e-81 254
Msa0158860 AT2G30010.2 37.568 370 198 8 35 375 74 439 4.60e-81 255
Msa0158860 AT1G73140.1 39.181 342 186 8 53 375 67 405 1.57e-80 253
Msa0158860 AT2G40160.2 38.040 347 191 9 53 375 77 423 1.81e-79 251
Msa0158860 AT3G14850.2 38.066 331 189 5 48 371 29 350 2.48e-79 248
Msa0158860 AT2G40160.1 37.752 347 192 9 53 375 77 423 3.49e-79 249
Msa0158860 AT1G60790.1 36.364 352 200 9 46 375 183 532 4.21e-79 253
Msa0158860 AT1G78710.1 34.897 341 203 7 43 375 28 357 9.77e-79 246
Msa0158860 AT3G55990.1 37.427 342 193 7 51 371 138 479 5.29e-77 246
Msa0158860 AT2G40150.1 36.888 347 192 7 52 371 70 416 5.51e-77 244
Msa0158860 AT2G38320.1 33.149 362 219 9 37 375 43 404 2.36e-75 239
Msa0158860 AT5G19160.1 36.486 370 203 8 32 375 81 444 2.47e-75 241
Msa0158860 AT5G01620.2 35.278 360 211 9 35 375 93 449 4.81e-74 237
Msa0158860 AT5G01620.1 35.278 360 211 9 35 375 93 449 4.81e-74 237
Msa0158860 AT5G01620.3 35.278 360 211 9 35 375 101 457 5.99e-74 237
Msa0158860 AT3G11570.1 35.606 396 214 12 8 375 37 419 3.78e-73 234
Msa0158860 AT3G06080.2 36.752 351 196 7 48 375 98 445 7.72e-71 229
Msa0158860 AT2G40320.1 34.568 405 219 13 8 375 27 422 1.90e-70 227
Msa0158860 AT5G06230.2 37.677 353 187 8 50 375 18 364 1.00e-69 223
Msa0158860 AT5G06230.1 37.815 357 181 10 50 375 59 405 1.70e-69 224
Msa0158860 AT3G11030.1 35.549 346 197 8 53 375 106 448 5.36e-69 224
Msa0158860 AT2G31110.3 40.230 261 151 4 13 270 3 261 5.91e-67 213
Msa0158860 AT1G48880.1 35.294 340 188 9 53 375 115 439 3.48e-66 216
Msa0158860 AT2G38320.2 33.022 321 194 7 76 375 1 321 4.84e-66 213
Msa0158860 AT2G30900.2 42.609 230 127 5 51 277 41 268 1.48e-63 204
Msa0158860 AT5G58600.2 40.678 236 134 4 44 277 60 291 1.36e-62 202
Msa0158860 AT3G62390.2 36.765 272 155 4 23 277 102 373 3.98e-62 205
Msa0158860 AT2G34070.2 37.308 260 146 4 122 375 1 249 2.50e-60 195
Msa0158860 AT2G40160.3 40.678 236 129 6 53 277 77 312 3.29e-59 194
Msa0158860 AT5G19160.2 37.066 259 147 4 32 277 81 336 6.99e-56 187
Msa0158860 AT5G20590.2 38.272 243 137 4 48 277 136 378 2.14e-55 186
Msa0158860 AT1G78710.2 30.532 357 180 11 23 375 5 297 8.11e-53 177
Msa0158860 AT3G06080.1 35.802 243 143 3 48 277 98 340 8.01e-50 171
Msa0158860 AT3G14850.1 35.938 256 149 4 122 371 1 247 2.82e-49 167
Msa0158860 AT2G31110.1 40.553 217 113 4 164 375 9 214 3.49e-49 165
Msa0158860 AT5G06230.3 37.652 247 135 4 50 277 59 305 5.30e-49 168
Msa0158860 AT1G70230.1 31.534 352 193 12 53 375 79 411 6.04e-42 152
Msa0158860 AT1G01430.1 28.378 370 228 12 37 375 84 447 4.73e-41 150
Msa0158860 AT4G25360.1 31.383 376 208 16 37 375 162 524 7.81e-41 151
Msa0158860 AT4G25360.2 31.383 376 208 16 37 375 162 524 7.81e-41 151
Msa0158860 AT4G01080.1 28.736 348 215 11 49 371 87 426 1.38e-39 146
Msa0158860 AT5G64020.1 29.050 358 213 15 53 375 57 408 1.13e-38 143
Msa0158860 AT5G51640.1 29.223 373 218 12 39 375 126 488 2.58e-37 141
Msa0158860 AT2G37720.1 29.096 354 210 14 51 375 141 482 1.33e-36 139
Msa0158860 AT5G20680.6 27.041 392 232 17 19 375 85 457 1.82e-36 138
Msa0158860 AT5G20680.2 27.273 385 226 17 26 375 168 533 3.58e-36 138
Msa0158860 AT5G64470.3 27.095 358 228 12 43 375 45 394 3.80e-36 136
Msa0158860 AT5G20680.4 27.273 385 226 17 26 375 186 551 4.70e-36 138
Msa0158860 AT5G20680.1 27.273 385 226 17 26 375 186 551 4.70e-36 138
Msa0158860 AT5G20680.3 27.273 385 226 17 26 375 186 551 4.70e-36 138
Msa0158860 AT5G20680.5 27.273 385 226 17 26 375 186 551 4.70e-36 138
Msa0158860 AT4G23790.1 27.559 381 215 13 34 371 61 423 4.81e-36 136
Msa0158860 AT5G64470.2 26.923 364 227 12 43 375 45 400 1.22e-35 135
Msa0158860 AT4G11090.1 29.477 363 204 15 51 375 77 425 1.50e-35 135
Msa0158860 AT5G15900.1 28.926 363 224 11 40 375 54 409 1.14e-33 130
Msa0158860 AT5G15900.2 28.926 363 224 11 40 375 73 428 1.69e-33 129
Msa0158860 AT5G15890.1 28.691 359 209 11 47 375 181 522 4.41e-33 129
Msa0158860 AT3G02440.2 28.125 352 214 12 53 375 135 476 6.21e-33 129
Msa0158860 AT3G02440.1 28.125 352 214 12 53 375 127 468 7.27e-33 128
Msa0158860 AT2G14530.1 27.701 361 213 13 50 375 59 406 3.15e-32 125
Msa0158860 AT3G28150.1 28.117 377 209 18 39 375 56 410 5.14e-31 122
Msa0158860 AT5G64470.4 26.848 257 169 7 43 282 45 299 4.26e-24 101
Msa0158860 AT5G64470.1 26.953 256 168 7 43 281 45 298 6.60e-24 101

Find 128 sgRNAs with CRISPR-Local

Find 291 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTACAAATGCTATTAGTTT+AGG 0.207769 1_4:+28749324 None:intergenic
TGCTAGATTCTCTATTGTTT+TGG 0.217386 1_4:+28752446 None:intergenic
TAATACCAACTGGTAGAAAA+AGG 0.249328 1_4:-28750711 Msa0158860:CDS
GCAGTCTGGTTTGTGTATCT+TGG 0.261971 1_4:-28749078 Msa0158860:three_prime_UTR
CTTATGGTTGCTTATCAAAA+AGG 0.263528 1_4:-28749999 Msa0158860:CDS
CCACTGCACATTATCTCTTC+TGG 0.264239 1_4:-28752521 Msa0158860:CDS
TTTGATGCACTAAGGTTTCT+TGG 0.268531 1_4:-28750820 Msa0158860:CDS
ACATGCTGACTAACAGTCTA+AGG 0.275525 1_4:+28749249 None:intergenic
TTACAAATGCTATTAGTTTA+GGG 0.278550 1_4:+28749325 None:intergenic
TCCCTGGTGTGCCTGATATA+TGG 0.296302 1_4:-28749403 Msa0158860:CDS
AACTAATAGCATTTGTAATT+AGG 0.297361 1_4:-28749320 Msa0158860:three_prime_UTR
GGTTCAATACCTTCTTTGTT+TGG 0.300281 1_4:-28748960 Msa0158860:three_prime_UTR
GCAGAAAGTTGTGCTAGTTT+TGG 0.302343 1_4:-28748981 Msa0158860:three_prime_UTR
TTCATCAAGGCATGATTTGC+GGG 0.320342 1_4:+28752685 None:intergenic
GTTACAATGCTGCCAACTTT+AGG 0.320362 1_4:+28749216 None:intergenic
GCTGAAGAAAATGAGGTTTC+CGG 0.326494 1_4:-28749555 Msa0158860:CDS
AAAATCATACTTATCCTATA+TGG 0.347597 1_4:+28749194 None:intergenic
GTTCAGGAGCATGTTGTTGC+AGG 0.359962 1_4:+28749600 None:intergenic
AACATCTCATTCCATATATC+AGG 0.370214 1_4:+28749392 None:intergenic
TAGGGTTATGTAGAGTGAAA+AGG 0.375327 1_4:+28752640 None:intergenic
TGGACAATGTTAACATCATC+AGG 0.381677 1_4:+28752466 None:intergenic
GCATGTCACCTAGGCATAAC+AGG 0.386311 1_4:-28749908 Msa0158860:intron
ATCGACACGGATGATTTGAT+TGG 0.394595 1_4:-28749130 Msa0158860:three_prime_UTR
AAACAAGAGAGTCTTACATT+TGG 0.395362 1_4:-28750505 Msa0158860:CDS
CATGCTTCTGAAAATGACTT+TGG 0.398420 1_4:+28749926 None:intergenic
ATTCATCTGATTGCAGTCAT+TGG 0.399784 1_4:-28749430 Msa0158860:CDS
CACTTCTCATAATCAAAATC+TGG 0.400310 1_4:+28752313 None:intergenic
TGAGTGAGTTAAGGAAGAGT+AGG 0.412926 1_4:+28752621 None:intergenic
ATTGAATGGAAGGCCATTGC+AGG 0.414061 1_4:+28750673 None:intergenic
AGGGTTATGTAGAGTGAAAA+GGG 0.414426 1_4:+28752641 None:intergenic
TGCAGTCATTGGTGCCTCCC+TGG 0.417455 1_4:-28749419 Msa0158860:CDS
GCGTTCTGCGGTGGTGGTGC+TGG 0.424696 1_4:-28749038 Msa0158860:three_prime_UTR
CTTACATTTGGATTTGATTG+AGG 0.428799 1_4:-28750493 Msa0158860:CDS
TCATTGTCGTAATATCTTGC+AGG 0.431750 1_4:+28749528 None:intergenic
ATGTCTTCATTACCATTGAA+TGG 0.434469 1_4:+28750659 None:intergenic
TGGACCCACAAGATGATGTT+GGG 0.437455 1_4:-28752501 Msa0158860:CDS
TCAATACCTTCTTTGTTTGG+TGG 0.439752 1_4:-28748957 Msa0158860:three_prime_UTR
AAAGAAAGCAAATGAGTCAT+TGG 0.443285 1_4:+28752565 None:intergenic
TTGAGGAGAATGCAAAGTAT+TGG 0.448978 1_4:-28750476 Msa0158860:CDS
GCAGTTACTTGTCAAAAGAA+TGG 0.450896 1_4:-28752340 Msa0158860:CDS
ATGATTTGATTGGGAAAAGT+TGG 0.453112 1_4:-28749120 Msa0158860:three_prime_UTR
TTCCATATATCAGGCACACC+AGG 0.460916 1_4:+28749401 None:intergenic
ATGGACATCCTTCAGTGTAT+AGG 0.463306 1_4:-28749490 Msa0158860:CDS
GAGTGAGTTAAGGAAGAGTA+GGG 0.463745 1_4:+28752622 None:intergenic
TGGCAGCATTGTAACCATAT+AGG 0.463752 1_4:-28749208 Msa0158860:three_prime_UTR
TGGAGAACAACTAAAGGTTC+AGG 0.465074 1_4:+28749584 None:intergenic
GTAACTGCAGTACTAAGATA+AGG 0.467342 1_4:+28752355 None:intergenic
TCGACACGGATGATTTGATT+GGG 0.468415 1_4:-28749129 Msa0158860:three_prime_UTR
AGTGTGAAGTGAGTGAGTTA+AGG 0.469980 1_4:+28752612 None:intergenic
TCTGTAGGTTTGATGCACTA+AGG 0.472754 1_4:-28750828 Msa0158860:intron
CTTGGATTCTGCAACTTGGT+TGG 0.480932 1_4:-28749060 Msa0158860:three_prime_UTR
TGCAATGGCCTTCCATTCAA+TGG 0.483402 1_4:-28750671 Msa0158860:intron
GTACTAAGATAAGGGCAGTT+AGG 0.483942 1_4:+28752364 None:intergenic
GACTGTTAGTCAGCATGTAA+TGG 0.486385 1_4:-28749244 Msa0158860:three_prime_UTR
TGATTGGGAAAAGTTGGTGG+AGG 0.487454 1_4:-28749114 Msa0158860:three_prime_UTR
ATTTGATTGGGAAAAGTTGG+TGG 0.488206 1_4:-28749117 Msa0158860:three_prime_UTR
TTTCATCAAGGCATGATTTG+CGG 0.492463 1_4:+28752684 None:intergenic
CAGTTACTTGTCAAAAGAAT+GGG 0.497842 1_4:-28752339 Msa0158860:CDS
CTGGACCCACAAGATGATGT+TGG 0.501831 1_4:-28752502 Msa0158860:CDS
GTACAAGGTGTAATACCAAC+TGG 0.502784 1_4:-28750721 Msa0158860:CDS
TGGTTGGCGTTCTGCGGTGG+TGG 0.508681 1_4:-28749044 Msa0158860:three_prime_UTR
GTATCAAAGCTGCTGCAGTC+TGG 0.509135 1_4:-28749092 Msa0158860:three_prime_UTR
TAACTGCAGTACTAAGATAA+GGG 0.511515 1_4:+28752356 None:intergenic
GTAATATCTTGCAGGTATAC+CGG 0.515454 1_4:+28749536 None:intergenic
CTTCATTACCATTGAATGGA+AGG 0.525435 1_4:+28750663 None:intergenic
TATTTGATTCAGCTCAGTGG+TGG 0.527529 1_4:-28750434 Msa0158860:CDS
GTATCTTGGATTCTGCAACT+TGG 0.528317 1_4:-28749064 Msa0158860:three_prime_UTR
CACTCCCTTAGCTTCTTCCA+TGG 0.528413 1_4:-28752593 Msa0158860:exon
TTGATTATGAGAAGTGGAAG+TGG 0.528886 1_4:-28752306 Msa0158860:CDS
TTCAATAATGAGAAATCAGT+GGG 0.529697 1_4:-28750761 Msa0158860:CDS
GGAAGTAAAGTGTTTCATCA+AGG 0.530205 1_4:+28752672 None:intergenic
GAAACCCAACATCATCTTGT+GGG 0.534722 1_4:+28752497 None:intergenic
TGTTGCCATGGAAGAAGCTA+AGG 0.535620 1_4:+28752588 None:intergenic
TTCTTCAGTTCAACTAACAA+TGG 0.540448 1_4:+28750537 None:intergenic
AATGACTGCAATCAGATGAA+TGG 0.543715 1_4:+28749432 None:intergenic
TTTCTGTGTAGGGGTTGAGG+GGG 0.545844 1_4:-28748930 Msa0158860:three_prime_UTR
CTGCGGTGGTGGTGCTGGTG+TGG 0.549841 1_4:-28749033 Msa0158860:three_prime_UTR
ACTGAGTACATGGGCAAGGT+GGG 0.549939 1_4:-28749976 Msa0158860:CDS
GGGCAGTTAGGATCATAAAG+AGG 0.560599 1_4:+28752376 None:intergenic
AGGAAGAGAATCATGTTGGT+GGG 0.564160 1_4:-28750787 Msa0158860:CDS
ACTCCTTGGAGAACAACTAA+AGG 0.569021 1_4:+28749578 None:intergenic
GGAAGGCCATTGCAGGTCCA+TGG 0.570480 1_4:+28750680 None:intergenic
GCAACTTGGTTGGCGTTCTG+CGG 0.571599 1_4:-28749050 Msa0158860:three_prime_UTR
GGCAGTTAGGATCATAAAGA+GGG 0.572794 1_4:+28752377 None:intergenic
GACCTACCATGGACCTGCAA+TGG 0.573638 1_4:-28750686 Msa0158860:CDS
GAGAAGGAAGAGAATCATGT+TGG 0.573655 1_4:-28750791 Msa0158860:CDS
ATATAGTACCTGTTATGCCT+AGG 0.573860 1_4:+28749900 None:intergenic
GTAATGGAAGGACCTAAAGT+TGG 0.576685 1_4:-28749228 Msa0158860:three_prime_UTR
AAAACTCCACCAAACAAAGA+AGG 0.578611 1_4:+28748951 None:intergenic
AAGGAGTGCTGAAGAAAATG+AGG 0.580576 1_4:-28749562 Msa0158860:CDS
ACTTGGTTGGCGTTCTGCGG+TGG 0.582346 1_4:-28749047 Msa0158860:three_prime_UTR
GAGTACATGGGCAAGGTGGG+TGG 0.582998 1_4:-28749973 Msa0158860:CDS
TCTTTAGTATGCTTAGTACA+AGG 0.583313 1_4:-28750736 Msa0158860:CDS
GAGAATGCAAAGTATTGGAG+AGG 0.588312 1_4:-28750471 Msa0158860:CDS
CTAGTGGTTGTTCTATCCCA+AGG 0.588736 1_4:-28752279 Msa0158860:intron
GACTGAGTACATGGGCAAGG+TGG 0.591699 1_4:-28749977 Msa0158860:CDS
ATGAGTCATTGGTGTTGCCA+TGG 0.591707 1_4:+28752576 None:intergenic
TTGTATTTGATTCAGCTCAG+TGG 0.592724 1_4:-28750437 Msa0158860:CDS
AGAGTGAAAAGGGTTGGTGT+TGG 0.593466 1_4:+28752651 None:intergenic
TTATGTAGAGTGAAAAGGGT+TGG 0.595226 1_4:+28752645 None:intergenic
GAACCTTTAGTTGTTCTCCA+AGG 0.596967 1_4:-28749581 Msa0158860:CDS
AGGATGTCCATCTCTGCGTA+AGG 0.608878 1_4:+28749502 None:intergenic
CATGCTGACTAACAGTCTAA+GGG 0.610101 1_4:+28749250 None:intergenic
AGAAACCCAACATCATCTTG+TGG 0.610318 1_4:+28752496 None:intergenic
GGCCATTGCAGGTCCATGGT+AGG 0.611844 1_4:+28750684 None:intergenic
GTTGCCTTCCTATACACTGA+AGG 0.612680 1_4:+28749482 None:intergenic
AAGGAAGAGAATCATGTTGG+TGG 0.613045 1_4:-28750788 Msa0158860:CDS
ATGACCGCCTTACGCAGAGA+TGG 0.614118 1_4:-28749509 Msa0158860:CDS
ATTCAATAATGAGAAATCAG+TGG 0.617219 1_4:-28750762 Msa0158860:CDS
ATACAGGTGGGATTACTACA+AGG 0.634100 1_4:-28750051 Msa0158860:intron
ACTGAGCTGAATCAAATACA+AGG 0.634640 1_4:+28750439 None:intergenic
GTTAGTCAGCATGTAATGGA+AGG 0.635674 1_4:-28749240 Msa0158860:three_prime_UTR
CTTGGGATAGAACAACCACT+AGG 0.637657 1_4:+28752280 None:intergenic
TCATCAAGGCATGATTTGCG+GGG 0.644214 1_4:+28752686 None:intergenic
TCCATATATCAGGCACACCA+GGG 0.647522 1_4:+28749402 None:intergenic
AGGTGGGATTACTACAAGGA+GGG 0.648410 1_4:-28750047 Msa0158860:CDS
AAGGACTGAGTACATGGGCA+AGG 0.650632 1_4:-28749980 Msa0158860:CDS
ATGTCCATCTCTGCGTAAGG+CGG 0.652540 1_4:+28749505 None:intergenic
CCAGAAGAGATAATGTGCAG+TGG 0.657294 1_4:+28752521 None:intergenic
TTACTTGTCAAAAGAATGGG+AGG 0.658568 1_4:-28752336 Msa0158860:CDS
ACATCCTTCAGTGTATAGGA+AGG 0.659861 1_4:-28749486 Msa0158860:CDS
GTTGCCATGGAAGAAGCTAA+GGG 0.675280 1_4:+28752589 None:intergenic
TCTAAGGGAAGTGTAACAGA+AGG 0.680249 1_4:+28749265 None:intergenic
CAGGTGGGATTACTACAAGG+AGG 0.703135 1_4:-28750048 Msa0158860:CDS
AAATTGATAATAGACTCACA+TGG 0.740776 1_4:+28748883 None:intergenic
AGATGAACGTGAAATCGACA+CGG 0.743119 1_4:-28749143 Msa0158860:three_prime_UTR
AAGTGGAAGTGGAAGCCTAG+TGG 0.748991 1_4:-28752295 Msa0158860:CDS
ATATATCAGGCACACCAGGG+AGG 0.777258 1_4:+28749405 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTGTTATTGTTTTTTATA+GGG - chr1_4:28749465-28749484 Msa0158860:CDS 10.0%
!!! CAAAATATAAATTTTATCTA+AGG - chr1_4:28749625-28749644 Msa0158860:CDS 10.0%
!!! TAATATACTTTTTTGTAATT+AGG - chr1_4:28752262-28752281 Msa0158860:intron 10.0%
!!! GATTTGTTATTGTTTTTTAT+AGG - chr1_4:28749464-28749483 Msa0158860:CDS 15.0%
!!! TTTGTTATTGTTTTTTATAG+GGG - chr1_4:28749466-28749485 Msa0158860:CDS 15.0%
!!! ACTTGTTAAAGATTAAAAAA+AGG + chr1_4:28749721-28749740 None:intergenic 15.0%
!!! AACTTTGTTTATCAATTAAA+TGG + chr1_4:28749783-28749802 None:intergenic 15.0%
!!! TAGTATATTCTAGATTTTTT+AGG - chr1_4:28750068-28750087 Msa0158860:intron 15.0%
!!! AGTATATTCTAGATTTTTTA+GGG - chr1_4:28750069-28750088 Msa0158860:intron 15.0%
!! AGTTCTTAAAATAAGTAAAA+TGG + chr1_4:28750562-28750581 None:intergenic 15.0%
!! CATTTACTTAAATACAAATT+TGG + chr1_4:28750645-28750664 None:intergenic 15.0%
!!! ACAAAAAAGTATATTAGTTT+AGG + chr1_4:28752258-28752277 None:intergenic 15.0%
!!! TTGTTATTGTTTTTTATAGG+GGG - chr1_4:28749467-28749486 Msa0158860:CDS 20.0%
!!! TTTTTTTAGAACTCAATATC+CGG + chr1_4:28749991-28750010 None:intergenic 20.0%
!!! ATTGAGTTCTAAAAAAAACA+TGG - chr1_4:28749994-28750013 Msa0158860:CDS 20.0%
!!! GTATATTCTAGATTTTTTAG+GGG - chr1_4:28750070-28750089 Msa0158860:intron 20.0%
!! AGATTTATCATTCAAATTTG+TGG - chr1_4:28750103-28750122 Msa0158860:intron 20.0%
!!! TTGTGTTATTGTAATTTGTA+CGG - chr1_4:28750281-28750300 Msa0158860:intron 20.0%
!! CTTAAAATAAGTAAAATGGA+AGG + chr1_4:28750558-28750577 None:intergenic 20.0%
!!! ACAACACCATAATATTTTAT+CGG - chr1_4:28751765-28751784 Msa0158860:intron 20.0%
!! TGATTTCCGATAAAATATTA+TGG + chr1_4:28751774-28751793 None:intergenic 20.0%
!!! TTACAAATGCTATTAGTTTA+GGG + chr1_4:28752229-28752248 None:intergenic 20.0%
!!! ATTACAAATGCTATTAGTTT+AGG + chr1_4:28752230-28752249 None:intergenic 20.0%
!! AACTAATAGCATTTGTAATT+AGG - chr1_4:28752231-28752250 Msa0158860:intron 20.0%
!! AAAATCATACTTATCCTATA+TGG + chr1_4:28752360-28752379 None:intergenic 20.0%
! TATAGAAGAAAACAAAAAGG+TGG + chr1_4:28749311-28749330 None:intergenic 25.0%
! GTGTATAGAAGAAAACAAAA+AGG + chr1_4:28749314-28749333 None:intergenic 25.0%
! TGTATTTACTTGGTCAAAAT+TGG - chr1_4:28749408-28749427 Msa0158860:CDS 25.0%
!! GGAAACAATTGACAAATTTT+AGG + chr1_4:28749700-28749719 None:intergenic 25.0%
! TTTATCAATTAAATGGATGC+AGG + chr1_4:28749776-28749795 None:intergenic 25.0%
!!! AGTTCTAAAAAAAACATGGT+TGG - chr1_4:28749998-28750017 Msa0158860:CDS 25.0%
!!! TTTTTTATCAACTCTGAGTA+GGG - chr1_4:28750161-28750180 Msa0158860:intron 25.0%
!! TATTGTAATTTGTACGGATA+TGG - chr1_4:28750287-28750306 Msa0158860:intron 25.0%
!! ACAAATATTTTCAGCCAAAA+TGG + chr1_4:28750478-28750497 None:intergenic 25.0%
!!! ATTTTGGCTGAAAATATTTG+TGG - chr1_4:28750477-28750496 Msa0158860:CDS 25.0%
! TTTGTGGATTCTTATTAATG+TGG - chr1_4:28750493-28750512 Msa0158860:CDS 25.0%
! AAAATAAGTAAAATGGAAGG+TGG + chr1_4:28750555-28750574 None:intergenic 25.0%
! GTTCTTGAAATTGTAAATGT+CGG + chr1_4:28750623-28750642 None:intergenic 25.0%
! GTAAATGAATTTCAACACTT+TGG - chr1_4:28750658-28750677 Msa0158860:intron 25.0%
! ATTCAATAATGAGAAATCAG+TGG - chr1_4:28750789-28750808 Msa0158860:CDS 25.0%
! TTCAATAATGAGAAATCAGT+GGG - chr1_4:28750790-28750809 Msa0158860:CDS 25.0%
! TGTCAATAATTTACAAAGGA+AGG + chr1_4:28750942-28750961 None:intergenic 25.0%
! GTGATGTCAATAATTTACAA+AGG + chr1_4:28750946-28750965 None:intergenic 25.0%
!! AGACATCAATTGAGTTTTTA+TGG - chr1_4:28750988-28751007 Msa0158860:intron 25.0%
!! GACATCAATTGAGTTTTTAT+GGG - chr1_4:28750989-28751008 Msa0158860:intron 25.0%
! AAACACTGAATTATGTAGTT+TGG + chr1_4:28751239-28751258 None:intergenic 25.0%
! TAACTATCTTAGAAAGCTTA+TGG - chr1_4:28751359-28751378 Msa0158860:intron 25.0%
! AACTATCTTAGAAAGCTTAT+GGG - chr1_4:28751360-28751379 Msa0158860:intron 25.0%
! GATAAAAAACATGACTCTTA+TGG - chr1_4:28751536-28751555 Msa0158860:intron 25.0%
!!! AAATGACTTTGGTTTTTTGA+GGG + chr1_4:28751617-28751636 None:intergenic 25.0%
!!! AAAATGACTTTGGTTTTTTG+AGG + chr1_4:28751618-28751637 None:intergenic 25.0%
!! GACAAAGATAAACAAGTTTT+AGG + chr1_4:28751688-28751707 None:intergenic 25.0%
! AATGCCAAAAACAAAACTAT+AGG + chr1_4:28751847-28751866 None:intergenic 25.0%
!!! AGTGATTGGATTTTTTATGT+AGG - chr1_4:28751878-28751897 Msa0158860:intron 25.0%
!!! GTGATTGGATTTTTTATGTA+GGG - chr1_4:28751879-28751898 Msa0158860:intron 25.0%
!!! GATTTTTTATGTAGGGAAAA+TGG - chr1_4:28751886-28751905 Msa0158860:intron 25.0%
!!! CTATTTTTTGTTTCTCATCT+TGG + chr1_4:28752098-28752117 None:intergenic 25.0%
! CAAGATGAGAAACAAAAAAT+AGG - chr1_4:28752096-28752115 Msa0158860:intron 25.0%
!! AAATTGATAATAGACTCACA+TGG + chr1_4:28752671-28752690 None:intergenic 25.0%
AAAGAAAGCAAATGAGTCAT+TGG + chr1_4:28748989-28749008 None:intergenic 30.0%
! TGCTAGATTCTCTATTGTTT+TGG + chr1_4:28749108-28749127 None:intergenic 30.0%
! AAAAGATGTGACTTTTCTGT+TGG - chr1_4:28749127-28749146 Msa0158860:three_prime_UTR 30.0%
! TGACTTTTCTGTTGGAAAAT+GGG - chr1_4:28749135-28749154 Msa0158860:three_prime_UTR 30.0%
TAACTGCAGTACTAAGATAA+GGG + chr1_4:28749198-28749217 None:intergenic 30.0%
CAGTTACTTGTCAAAAGAAT+GGG - chr1_4:28749212-28749231 Msa0158860:three_prime_UTR 30.0%
CACTTCTCATAATCAAAATC+TGG + chr1_4:28749241-28749260 None:intergenic 30.0%
!!! CAGATTTTGATTATGAGAAG+TGG - chr1_4:28749239-28749258 Msa0158860:three_prime_UTR 30.0%
AGTAAATACATGTGTCATGA+TGG + chr1_4:28749398-28749417 None:intergenic 30.0%
TCATGACACATGTATTTACT+TGG - chr1_4:28749398-28749417 Msa0158860:CDS 30.0%
! GTATCACAATCTTGTGATTT+TGG + chr1_4:28749493-28749512 None:intergenic 30.0%
ATCACAAGATTGTGATACTT+AGG - chr1_4:28749495-28749514 Msa0158860:CDS 30.0%
TCACAAGATTGTGATACTTA+GGG - chr1_4:28749496-28749515 Msa0158860:CDS 30.0%
! CATAGGTTTAATTGCAGTTT+TGG + chr1_4:28749524-28749543 None:intergenic 30.0%
TTTCATGTGCATAACAATGA+TGG + chr1_4:28749549-28749568 None:intergenic 30.0%
CTTATTAACAAATCCATGAG+AGG + chr1_4:28749603-28749622 None:intergenic 30.0%
GATTCCTTCTGTATCAATTT+CGG - chr1_4:28749873-28749892 Msa0158860:intron 30.0%
TAATCTCATTGTTCACTTGT+GGG + chr1_4:28749941-28749960 None:intergenic 30.0%
ATTAGATCTCTCGAATTAGT+CGG - chr1_4:28749957-28749976 Msa0158860:CDS 30.0%
!!! GTTTTTTATCAACTCTGAGT+AGG - chr1_4:28750160-28750179 Msa0158860:intron 30.0%
! TTTTTATCAACTCTGAGTAG+GGG - chr1_4:28750162-28750181 Msa0158860:intron 30.0%
! TAAAATCTGAGATCAATGGA+GGG + chr1_4:28750221-28750240 None:intergenic 30.0%
! TTAAAATCTGAGATCAATGG+AGG + chr1_4:28750222-28750241 None:intergenic 30.0%
! GGATTAAAATCTGAGATCAA+TGG + chr1_4:28750225-28750244 None:intergenic 30.0%
AAGTTATGCATATAGGAGAT+GGG + chr1_4:28750246-28750265 None:intergenic 30.0%
ACTATGGAAGTTATGCATAT+AGG + chr1_4:28750253-28750272 None:intergenic 30.0%
TAACACAAAAACCATGACTA+TGG + chr1_4:28750269-28750288 None:intergenic 30.0%
GACTAACACTTACAATTTCA+GGG + chr1_4:28750362-28750381 None:intergenic 30.0%
TGACTAACACTTACAATTTC+AGG + chr1_4:28750363-28750382 None:intergenic 30.0%
! TTGCCTTTTCATGAAATTTG+GGG + chr1_4:28750393-28750412 None:intergenic 30.0%
! GTTGCCTTTTCATGAAATTT+GGG + chr1_4:28750394-28750413 None:intergenic 30.0%
! AGTTGCCTTTTCATGAAATT+TGG + chr1_4:28750395-28750414 None:intergenic 30.0%
TCTTTAGTATGCTTAGTACA+AGG - chr1_4:28750815-28750834 Msa0158860:CDS 30.0%
TAATACCAACTGGTAGAAAA+AGG - chr1_4:28750840-28750859 Msa0158860:intron 30.0%
AATACCAACTGGTAGAAAAA+GGG - chr1_4:28750841-28750860 Msa0158860:intron 30.0%
ATGTCTTCATTACCATTGAA+TGG + chr1_4:28750895-28750914 None:intergenic 30.0%
TTCTTCAGTTCAACTAACAA+TGG + chr1_4:28751017-28751036 None:intergenic 30.0%
AAACAAGAGAGTCTTACATT+TGG - chr1_4:28751046-28751065 Msa0158860:intron 30.0%
CTTACATTTGGATTTGATTG+AGG - chr1_4:28751058-28751077 Msa0158860:intron 30.0%
! TCTATTTTCTCAATTTCCCT+TGG - chr1_4:28751212-28751231 Msa0158860:intron 30.0%
! CTATTTTCTCAATTTCCCTT+GGG - chr1_4:28751213-28751232 Msa0158860:intron 30.0%
AATAAGCCAAAATCAGCTTA+TGG - chr1_4:28751383-28751402 Msa0158860:intron 30.0%
CTTATGGTTGCTTATCAAAA+AGG - chr1_4:28751552-28751571 Msa0158860:intron 30.0%
ACTTAGTGAGATATAAGACT+TGG + chr1_4:28751724-28751743 None:intergenic 30.0%
! ACTAAGTGAAGTTGATTACA+TGG - chr1_4:28751737-28751756 Msa0158860:intron 30.0%
GTTAAGATTTAGAAGCTCAA+TGG + chr1_4:28751815-28751834 None:intergenic 30.0%
!!! TTCGCCTATAGTTTTGTTTT+TGG - chr1_4:28751840-28751859 Msa0158860:intron 30.0%
ATTTCATTGTCTCTAGTGAT+TGG - chr1_4:28751864-28751883 Msa0158860:intron 30.0%
! TTTTATGTAGGGAAAATGGT+TGG - chr1_4:28751890-28751909 Msa0158860:intron 30.0%
AACATCTCATTCCATATATC+AGG + chr1_4:28752162-28752181 None:intergenic 30.0%
ATGATTTGATTGGGAAAAGT+TGG - chr1_4:28752431-28752450 Msa0158860:CDS 30.0%
TTTCATCAAGGCATGATTTG+CGG + chr1_4:28748870-28748889 None:intergenic 35.0%
! GGAAGTAAAGTGTTTCATCA+AGG + chr1_4:28748882-28748901 None:intergenic 35.0%
TTATGTAGAGTGAAAAGGGT+TGG + chr1_4:28748909-28748928 None:intergenic 35.0%
AGGGTTATGTAGAGTGAAAA+GGG + chr1_4:28748913-28748932 None:intergenic 35.0%
TAGGGTTATGTAGAGTGAAA+AGG + chr1_4:28748914-28748933 None:intergenic 35.0%
TGGACAATGTTAACATCATC+AGG + chr1_4:28749088-28749107 None:intergenic 35.0%
! GTGACTTTTCTGTTGGAAAA+TGG - chr1_4:28749134-28749153 Msa0158860:three_prime_UTR 35.0%
GTAACTGCAGTACTAAGATA+AGG + chr1_4:28749199-28749218 None:intergenic 35.0%
GCAGTTACTTGTCAAAAGAA+TGG - chr1_4:28749211-28749230 Msa0158860:three_prime_UTR 35.0%
TTACTTGTCAAAAGAATGGG+AGG - chr1_4:28749215-28749234 Msa0158860:three_prime_UTR 35.0%
TTGATTATGAGAAGTGGAAG+TGG - chr1_4:28749245-28749264 Msa0158860:three_prime_UTR 35.0%
! CAAAAGGGTGTAAAGTTAGA+GGG - chr1_4:28749439-28749458 Msa0158860:CDS 35.0%
CACAAGATTGTGATACTTAG+GGG - chr1_4:28749497-28749516 Msa0158860:CDS 35.0%
ACAAGATTGTGATACTTAGG+GGG - chr1_4:28749498-28749517 Msa0158860:CDS 35.0%
!! GCATAACAATGATGGATCAT+AGG + chr1_4:28749541-28749560 None:intergenic 35.0%
TAATCTTTAACAAGTGCCCT+CGG - chr1_4:28749726-28749745 Msa0158860:intron 35.0%
GATCTCAAATCAACGTCAAT+AGG + chr1_4:28749822-28749841 None:intergenic 35.0%
! CTTAGAGTGTGCTTTTCTTA+AGG - chr1_4:28749841-28749860 Msa0158860:intron 35.0%
AATCTCATTGTTCACTTGTG+GGG + chr1_4:28749940-28749959 None:intergenic 35.0%
CTAATCTCATTGTTCACTTG+TGG + chr1_4:28749942-28749961 None:intergenic 35.0%
TACTATGACTACTTGTGTCA+AGG + chr1_4:28750053-28750072 None:intergenic 35.0%
GAAGTTATGCATATAGGAGA+TGG + chr1_4:28750247-28750266 None:intergenic 35.0%
ATGCATAACTTCCATAGTCA+TGG - chr1_4:28750255-28750274 Msa0158860:intron 35.0%
! GTGGATGATGAGTTTTTGAA+TGG - chr1_4:28750317-28750336 Msa0158860:intron 35.0%
AGTCCCCAAATTTCATGAAA+AGG - chr1_4:28750387-28750406 Msa0158860:intron 35.0%
AGCTAGTTAAGTTTACAAGC+AGG + chr1_4:28750696-28750715 None:intergenic 35.0%
! AACTAGCTTATTACCTCTGT+AGG - chr1_4:28750708-28750727 Msa0158860:CDS 35.0%
TTTGATGCACTAAGGTTTCT+TGG - chr1_4:28750731-28750750 Msa0158860:CDS 35.0%
GGTTTCTTGGAAAAATGAGA+AGG - chr1_4:28750744-28750763 Msa0158860:CDS 35.0%
CTTCATTACCATTGAATGGA+AGG + chr1_4:28750891-28750910 None:intergenic 35.0%
! ATTGACATCACAGTTTTCTC+TGG - chr1_4:28750955-28750974 Msa0158860:intron 35.0%
!!! TGTAAGACTCTCTTGTTTTC+TGG + chr1_4:28751044-28751063 None:intergenic 35.0%
TTGAGGAGAATGCAAAGTAT+TGG - chr1_4:28751075-28751094 Msa0158860:intron 35.0%
ACTGAGCTGAATCAAATACA+AGG + chr1_4:28751115-28751134 None:intergenic 35.0%
TTGTATTTGATTCAGCTCAG+TGG - chr1_4:28751114-28751133 Msa0158860:intron 35.0%
!!! TCCTTACGAGCTTGTTTTAT+CGG + chr1_4:28751150-28751169 None:intergenic 35.0%
AATAGAACGATGTGAAGAGA+AGG + chr1_4:28751198-28751217 None:intergenic 35.0%
AATTATGTAGTTTGGCCCAA+GGG + chr1_4:28751231-28751250 None:intergenic 35.0%
CAAAATCAGCTTATGGACAT+GGG - chr1_4:28751390-28751409 Msa0158860:intron 35.0%
!! GTCGATTAGCTTTCTAAAAG+AGG - chr1_4:28751422-28751441 Msa0158860:intron 35.0%
GCTTCTTTCTTGACTAATAC+AGG - chr1_4:28751484-28751503 Msa0158860:intron 35.0%
TCTTTCTTGACTAATACAGG+TGG - chr1_4:28751487-28751506 Msa0158860:intron 35.0%
CTTTCTTGACTAATACAGGT+GGG - chr1_4:28751488-28751507 Msa0158860:intron 35.0%
ATCAAAAAGGACTGAGTACA+TGG - chr1_4:28751565-28751584 Msa0158860:intron 35.0%
TCAAAAAGGACTGAGTACAT+GGG - chr1_4:28751566-28751585 Msa0158860:intron 35.0%
! CATGCTTCTGAAAATGACTT+TGG + chr1_4:28751628-28751647 None:intergenic 35.0%
ATATAGTACCTGTTATGCCT+AGG + chr1_4:28751654-28751673 None:intergenic 35.0%
GTAATATCTTGCAGGTATAC+CGG + chr1_4:28752018-28752037 None:intergenic 35.0%
TCATTGTCGTAATATCTTGC+AGG + chr1_4:28752026-28752045 None:intergenic 35.0%
!! TTTTTGTTTCTCATCTTGGC+TGG + chr1_4:28752094-28752113 None:intergenic 35.0%
AATGACTGCAATCAGATGAA+TGG + chr1_4:28752122-28752141 None:intergenic 35.0%
ATTCATCTGATTGCAGTCAT+TGG - chr1_4:28752121-28752140 Msa0158860:intron 35.0%
ATTTGATTGGGAAAAGTTGG+TGG - chr1_4:28752434-28752453 Msa0158860:CDS 35.0%
GGTTCAATACCTTCTTTGTT+TGG - chr1_4:28752591-28752610 Msa0158860:exon 35.0%
! TCAATACCTTCTTTGTTTGG+TGG - chr1_4:28752594-28752613 Msa0158860:exon 35.0%
AAAACTCCACCAAACAAAGA+AGG + chr1_4:28752603-28752622 None:intergenic 35.0%
GAGTGAGTTAAGGAAGAGTA+GGG + chr1_4:28748932-28748951 None:intergenic 40.0%
TGAGTGAGTTAAGGAAGAGT+AGG + chr1_4:28748933-28748952 None:intergenic 40.0%
AGTGTGAAGTGAGTGAGTTA+AGG + chr1_4:28748942-28748961 None:intergenic 40.0%
GAAACCCAACATCATCTTGT+GGG + chr1_4:28749057-28749076 None:intergenic 40.0%
AGAAACCCAACATCATCTTG+TGG + chr1_4:28749058-28749077 None:intergenic 40.0%
GGCAGTTAGGATCATAAAGA+GGG + chr1_4:28749177-28749196 None:intergenic 40.0%
GTACTAAGATAAGGGCAGTT+AGG + chr1_4:28749190-28749209 None:intergenic 40.0%
! ACTTGGTCAAAATTGGTCTG+TGG - chr1_4:28749415-28749434 Msa0158860:CDS 40.0%
! AAAATTGGTCTGTGGCCAAA+AGG - chr1_4:28749423-28749442 Msa0158860:CDS 40.0%
! AAATTGGTCTGTGGCCAAAA+GGG - chr1_4:28749424-28749443 Msa0158860:CDS 40.0%
! CCTCTAACTTTACACCCTTT+TGG + chr1_4:28749441-28749460 None:intergenic 40.0%
! CCAAAAGGGTGTAAAGTTAG+AGG - chr1_4:28749438-28749457 Msa0158860:CDS 40.0%
AATGTTAACGTGTCCTCTCA+TGG - chr1_4:28749587-28749606 Msa0158860:CDS 40.0%
TGAAATTCTAAGTGCTCCGA+GGG + chr1_4:28749745-28749764 None:intergenic 40.0%
TTGAAATTCTAAGTGCTCCG+AGG + chr1_4:28749746-28749765 None:intergenic 40.0%
TCCTTCTGTATCAATTTCGG+TGG - chr1_4:28749876-28749895 Msa0158860:intron 40.0%
TCCACCGAAATTGATACAGA+AGG + chr1_4:28749880-28749899 None:intergenic 40.0%
ATCTCATTGTTCACTTGTGG+GGG + chr1_4:28749939-28749958 None:intergenic 40.0%
TCTCGAATTAGTCGGTCTTT+AGG - chr1_4:28749965-28749984 Msa0158860:CDS 40.0%
! ACAACTCACAAGGACCATTT+TGG - chr1_4:28750461-28750480 Msa0158860:CDS 40.0%
TTAGTGCATCAAACCTACAG+AGG + chr1_4:28750724-28750743 None:intergenic 40.0%
TCTGTAGGTTTGATGCACTA+AGG - chr1_4:28750723-28750742 Msa0158860:CDS 40.0%
GAGAAGGAAGAGAATCATGT+TGG - chr1_4:28750760-28750779 Msa0158860:CDS 40.0%
AAGGAAGAGAATCATGTTGG+TGG - chr1_4:28750763-28750782 Msa0158860:CDS 40.0%
AGGAAGAGAATCATGTTGGT+GGG - chr1_4:28750764-28750783 Msa0158860:CDS 40.0%
GTACAAGGTGTAATACCAAC+TGG - chr1_4:28750830-28750849 Msa0158860:intron 40.0%
! ACATCACAGTTTTCTCTGGT+AGG - chr1_4:28750959-28750978 Msa0158860:intron 40.0%
!! GAGAATGCAAAGTATTGGAG+AGG - chr1_4:28751080-28751099 Msa0158860:intron 40.0%
TATTTGATTCAGCTCAGTGG+TGG - chr1_4:28751117-28751136 Msa0158860:intron 40.0%
TCCGATAAAACAAGCTCGTA+AGG - chr1_4:28751146-28751165 Msa0158860:intron 40.0%
GAATTATGTAGTTTGGCCCA+AGG + chr1_4:28751232-28751251 None:intergenic 40.0%
!! CCATGTCCATAAGCTGATTT+TGG + chr1_4:28751392-28751411 None:intergenic 40.0%
CCAAAATCAGCTTATGGACA+TGG - chr1_4:28751389-28751408 Msa0158860:intron 40.0%
ATACAGGTGGGATTACTACA+AGG - chr1_4:28751500-28751519 Msa0158860:intron 40.0%
!! TTTTCAGAAGCATGTCACCT+AGG - chr1_4:28751634-28751653 Msa0158860:intron 40.0%
AGATTTAGAAGCTCAATGGC+TGG + chr1_4:28751811-28751830 None:intergenic 40.0%
TGTTGTTGCAGGCTGAAAAA+TGG + chr1_4:28751943-28751962 None:intergenic 40.0%
TGGAGAACAACTAAAGGTTC+AGG + chr1_4:28751970-28751989 None:intergenic 40.0%
GAACCTTTAGTTGTTCTCCA+AGG - chr1_4:28751970-28751989 Msa0158860:intron 40.0%
ACTCCTTGGAGAACAACTAA+AGG + chr1_4:28751976-28751995 None:intergenic 40.0%
! TCATTTTCTTCAGCACTCCT+TGG + chr1_4:28751990-28752009 None:intergenic 40.0%
!! AAGGAGTGCTGAAGAAAATG+AGG - chr1_4:28751989-28752008 Msa0158860:intron 40.0%
GCTGAAGAAAATGAGGTTTC+CGG - chr1_4:28751996-28752015 Msa0158860:intron 40.0%
ATGGACATCCTTCAGTGTAT+AGG - chr1_4:28752061-28752080 Msa0158860:intron 40.0%
ACATCCTTCAGTGTATAGGA+AGG - chr1_4:28752065-28752084 Msa0158860:intron 40.0%
! TCTAAGGGAAGTGTAACAGA+AGG + chr1_4:28752289-28752308 None:intergenic 40.0%
! CATGCTGACTAACAGTCTAA+GGG + chr1_4:28752304-28752323 None:intergenic 40.0%
! ACATGCTGACTAACAGTCTA+AGG + chr1_4:28752305-28752324 None:intergenic 40.0%
GACTGTTAGTCAGCATGTAA+TGG - chr1_4:28752307-28752326 Msa0158860:CDS 40.0%
! GTTAGTCAGCATGTAATGGA+AGG - chr1_4:28752311-28752330 Msa0158860:CDS 40.0%
! GTAATGGAAGGACCTAAAGT+TGG - chr1_4:28752323-28752342 Msa0158860:CDS 40.0%
GTTACAATGCTGCCAACTTT+AGG + chr1_4:28752338-28752357 None:intergenic 40.0%
TGGCAGCATTGTAACCATAT+AGG - chr1_4:28752343-28752362 Msa0158860:CDS 40.0%
AGATGAACGTGAAATCGACA+CGG - chr1_4:28752408-28752427 Msa0158860:CDS 40.0%
ATCGACACGGATGATTTGAT+TGG - chr1_4:28752421-28752440 Msa0158860:CDS 40.0%
TCGACACGGATGATTTGATT+GGG - chr1_4:28752422-28752441 Msa0158860:CDS 40.0%
GTATCTTGGATTCTGCAACT+TGG - chr1_4:28752487-28752506 Msa0158860:CDS 40.0%
!! GCAGAAAGTTGTGCTAGTTT+TGG - chr1_4:28752570-28752589 Msa0158860:CDS 40.0%
! TTGGTGGAGTTTTTCTGTGT+AGG - chr1_4:28752610-28752629 Msa0158860:five_prime_UTR 40.0%
! TGGTGGAGTTTTTCTGTGTA+GGG - chr1_4:28752611-28752630 Msa0158860:five_prime_UTR 40.0%
! TTTTTCTGTGTAGGGGTTGA+GGG - chr1_4:28752619-28752638 Msa0158860:five_prime_UTR 40.0%
!! AGAGTGAAAAGGGTTGGTGT+TGG + chr1_4:28748903-28748922 None:intergenic 45.0%
GTTGCCATGGAAGAAGCTAA+GGG + chr1_4:28748965-28748984 None:intergenic 45.0%
TGTTGCCATGGAAGAAGCTA+AGG + chr1_4:28748966-28748985 None:intergenic 45.0%
!! ATGAGTCATTGGTGTTGCCA+TGG + chr1_4:28748978-28748997 None:intergenic 45.0%
CCAGAAGAGATAATGTGCAG+TGG + chr1_4:28749033-28749052 None:intergenic 45.0%
CCACTGCACATTATCTCTTC+TGG - chr1_4:28749030-28749049 Msa0158860:three_prime_UTR 45.0%
TGGACCCACAAGATGATGTT+GGG - chr1_4:28749050-28749069 Msa0158860:three_prime_UTR 45.0%
GGGCAGTTAGGATCATAAAG+AGG + chr1_4:28749178-28749197 None:intergenic 45.0%
CTTGGGATAGAACAACCACT+AGG + chr1_4:28749274-28749293 None:intergenic 45.0%
CTAGTGGTTGTTCTATCCCA+AGG - chr1_4:28749272-28749291 Msa0158860:three_prime_UTR 45.0%
TGGCAATGTGAACAGACCTT+GGG + chr1_4:28749291-28749310 None:intergenic 45.0%
GAATTAGTCGGTCTTTAGGC+CGG - chr1_4:28749969-28749988 Msa0158860:CDS 45.0%
! ATCTGAGATCAATGGAGGGT+TGG + chr1_4:28750217-28750236 None:intergenic 45.0%
GTACGGATATGGTAAGCTTG+TGG - chr1_4:28750298-28750317 Msa0158860:intron 45.0%
TTGCGAAGTCACAACTCACA+AGG - chr1_4:28750451-28750470 Msa0158860:CDS 45.0%
! GTCACCCTTTTTCTACCAGT+TGG + chr1_4:28750848-28750867 None:intergenic 45.0%
AGAAAAAGGGTGACCTACCA+TGG - chr1_4:28750854-28750873 Msa0158860:intron 45.0%
ATTGAATGGAAGGCCATTGC+AGG + chr1_4:28750881-28750900 None:intergenic 45.0%
TGCAATGGCCTTCCATTCAA+TGG - chr1_4:28750880-28750899 Msa0158860:intron 45.0%
AGAACGATGTGAAGAGAAGG+TGG + chr1_4:28751195-28751214 None:intergenic 45.0%
AGGTGGGATTACTACAAGGA+GGG - chr1_4:28751504-28751523 Msa0158860:intron 45.0%
! TAGAAGCTCAATGGCTGGTT+TGG + chr1_4:28751806-28751825 None:intergenic 45.0%
TGCAGGCTGAAAAATGGTAG+AGG + chr1_4:28751937-28751956 None:intergenic 45.0%
GTTGCCTTCCTATACACTGA+AGG + chr1_4:28752072-28752091 None:intergenic 45.0%
TCCATATATCAGGCACACCA+GGG + chr1_4:28752152-28752171 None:intergenic 45.0%
TTCCATATATCAGGCACACC+AGG + chr1_4:28752153-28752172 None:intergenic 45.0%
!! TGATTGGGAAAAGTTGGTGG+AGG - chr1_4:28752437-28752456 Msa0158860:CDS 45.0%
! GCAGTCTGGTTTGTGTATCT+TGG - chr1_4:28752473-28752492 Msa0158860:CDS 45.0%
CTTGGATTCTGCAACTTGGT+TGG - chr1_4:28752491-28752510 Msa0158860:CDS 45.0%
! GGTGGAGTTTTTCTGTGTAG+GGG - chr1_4:28752612-28752631 Msa0158860:five_prime_UTR 45.0%
! GTTTTTCTGTGTAGGGGTTG+AGG - chr1_4:28752618-28752637 Msa0158860:five_prime_UTR 45.0%
! TTTTCTGTGTAGGGGTTGAG+GGG - chr1_4:28752620-28752639 Msa0158860:five_prime_UTR 45.0%
CACTCCCTTAGCTTCTTCCA+TGG - chr1_4:28748958-28748977 Msa0158860:three_prime_UTR 50.0%
CTGGACCCACAAGATGATGT+TGG - chr1_4:28749049-28749068 Msa0158860:three_prime_UTR 50.0%
AAGTGGAAGTGGAAGCCTAG+TGG - chr1_4:28749256-28749275 Msa0158860:three_prime_UTR 50.0%
GTGGCAATGTGAACAGACCT+TGG + chr1_4:28749292-28749311 None:intergenic 50.0%
ATCAACTCTGAGTAGGGGAG+TGG - chr1_4:28750167-28750186 Msa0158860:intron 50.0%
TGAGTAGGGGAGTGGAATGT+GGG - chr1_4:28750175-28750194 Msa0158860:intron 50.0%
CAGGTGGGATTACTACAAGG+AGG - chr1_4:28751503-28751522 Msa0158860:intron 50.0%
AAGGACTGAGTACATGGGCA+AGG - chr1_4:28751571-28751590 Msa0158860:intron 50.0%
ACTGAGTACATGGGCAAGGT+GGG - chr1_4:28751575-28751594 Msa0158860:intron 50.0%
GCATGTCACCTAGGCATAAC+AGG - chr1_4:28751643-28751662 Msa0158860:intron 50.0%
! GTTCAGGAGCATGTTGTTGC+AGG + chr1_4:28751954-28751973 None:intergenic 50.0%
ATGTCCATCTCTGCGTAAGG+CGG + chr1_4:28752049-28752068 None:intergenic 50.0%
AGGATGTCCATCTCTGCGTA+AGG + chr1_4:28752052-28752071 None:intergenic 50.0%
ATATATCAGGCACACCAGGG+AGG + chr1_4:28752149-28752168 None:intergenic 50.0%
TCCCTGGTGTGCCTGATATA+TGG - chr1_4:28752148-28752167 Msa0158860:intron 50.0%
GTATCAAAGCTGCTGCAGTC+TGG - chr1_4:28752459-28752478 Msa0158860:CDS 50.0%
TTTCTGTGTAGGGGTTGAGG+GGG - chr1_4:28752621-28752640 Msa0158860:five_prime_UTR 50.0%
CTGAGTAGGGGAGTGGAATG+TGG - chr1_4:28750174-28750193 Msa0158860:intron 55.0%
GACCTACCATGGACCTGCAA+TGG - chr1_4:28750865-28750884 Msa0158860:intron 55.0%
GACTGAGTACATGGGCAAGG+TGG - chr1_4:28751574-28751593 Msa0158860:intron 55.0%
ATGACCGCCTTACGCAGAGA+TGG - chr1_4:28752042-28752061 Msa0158860:intron 55.0%
GCAACTTGGTTGGCGTTCTG+CGG - chr1_4:28752501-28752520 Msa0158860:CDS 55.0%
GGCCATTGCAGGTCCATGGT+AGG + chr1_4:28750870-28750889 None:intergenic 60.0%
GGAAGGCCATTGCAGGTCCA+TGG + chr1_4:28750874-28750893 None:intergenic 60.0%
GAGTACATGGGCAAGGTGGG+TGG - chr1_4:28751578-28751597 Msa0158860:intron 60.0%
! TGCAGTCATTGGTGCCTCCC+TGG - chr1_4:28752132-28752151 Msa0158860:intron 60.0%
ACTTGGTTGGCGTTCTGCGG+TGG - chr1_4:28752504-28752523 Msa0158860:CDS 60.0%
TGTAGGGGTTGAGGGGGCTT+TGG - chr1_4:28752627-28752646 Msa0158860:five_prime_UTR 60.0%
! GTTGAGGGGGCTTTGGTGTG+TGG - chr1_4:28752634-28752653 Msa0158860:five_prime_UTR 60.0%
TGGTTGGCGTTCTGCGGTGG+TGG - chr1_4:28752507-28752526 Msa0158860:CDS 65.0%
! GCGTTCTGCGGTGGTGGTGC+TGG - chr1_4:28752513-28752532 Msa0158860:CDS 70.0%
!! CTGCGGTGGTGGTGCTGGTG+TGG - chr1_4:28752518-28752537 Msa0158860:CDS 70.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 28748867 28752706 28748867 ID=Msa0158860;Name=Msa0158860
chr1_4 mRNA 28748867 28752706 28748867 ID=Msa0158860-mRNA-1;Parent=Msa0158860;Name=Msa0158860-mRNA-1;_AED=0.12;_eAED=0.12;_QI=110|1|1|1|1|1|5|509|375
chr1_4 exon 28748867 28749673 28748867 ID=Msa0158860-mRNA-1:exon:9251;Parent=Msa0158860-mRNA-1
chr1_4 exon 28749909 28750067 28749909 ID=Msa0158860-mRNA-1:exon:9250;Parent=Msa0158860-mRNA-1
chr1_4 exon 28750411 28750592 28750411 ID=Msa0158860-mRNA-1:exon:9249;Parent=Msa0158860-mRNA-1
chr1_4 exon 28750672 28750843 28750672 ID=Msa0158860-mRNA-1:exon:9248;Parent=Msa0158860-mRNA-1
chr1_4 exon 28752280 28752706 28752280 ID=Msa0158860-mRNA-1:exon:9247;Parent=Msa0158860-mRNA-1
chr1_4 five_prime_UTR 28752597 28752706 28752597 ID=Msa0158860-mRNA-1:five_prime_utr;Parent=Msa0158860-mRNA-1
chr1_4 CDS 28752280 28752596 28752280 ID=Msa0158860-mRNA-1:cds;Parent=Msa0158860-mRNA-1
chr1_4 CDS 28750672 28750843 28750672 ID=Msa0158860-mRNA-1:cds;Parent=Msa0158860-mRNA-1
chr1_4 CDS 28750411 28750592 28750411 ID=Msa0158860-mRNA-1:cds;Parent=Msa0158860-mRNA-1
chr1_4 CDS 28749909 28750067 28749909 ID=Msa0158860-mRNA-1:cds;Parent=Msa0158860-mRNA-1
chr1_4 CDS 28749376 28749673 28749376 ID=Msa0158860-mRNA-1:cds;Parent=Msa0158860-mRNA-1
chr1_4 three_prime_UTR 28748867 28749375 28748867 ID=Msa0158860-mRNA-1:three_prime_utr;Parent=Msa0158860-mRNA-1
Gene Sequence

>Msa0158860

ATGGCAACACCAATGACTCATTTGCTTTCTTTTTTCTTGCTGTTCAGCATCTTCCACTGCACATTATCTCTTCTGGACCCACAAGATGATGTTGGGTTTCTAGTTGAACCTGATGATGTTAACATTGTCCAAAACAATAGAGAATCTAGCAAAAGATGTGACTTTTCTGTTGGAAAATGGGTTTATGATGAATCTTACCCTCTTTATGATCCTAACTGCCCTTATCTTAGTACTGCAGTTACTTGTCAAAAGAATGGGAGGCCAGATTTTGATTATGAGAAGTGGAAGTGGAAGCCTAGTGGTTGTTCTATCCCAAGGTTTGATGCACTAAGGTTTCTTGGAAAAATGAGAAGGAAGAGAATCATGTTGGTGGGTGATTCAATAATGAGAAATCAGTGGGAATCTTTAGTATGCTTAGTACAAGGTGTAATACCAACTGGTAGAAAAAGGGTGACCTACCATGGACCTGCAATGGCCTTCCATTCAATGGATTTTGAGACATCAATTGAGTTTTTATGGGCACCATTGTTAGTTGAACTGAAGAAAAGTCCAGAAAACAAGAGAGTCTTACATTTGGATTTGATTGAGGAGAATGCAAAGTATTGGAGAGGAGTTGATATCCTTGTATTTGATTCAGCTCAGTGGTGGACTCACTCCGATAAAACAAGCTCGTGGGATTACTACAAGGAGGGAAATAATCTGATAAAAAACATGACTCTTATGGTTGCTTATCAAAAAGGACTGAGTACATGGGCAAGGTGGGTGGATCTAAATCTAAACCCTCAAAAAACCAAAGTCATTTTCAGAAGCATGTCACCTAGGCATAACAGGGAAAATGGTTGGAAATGTGACAATCAGAAACAACCTCTACCATTTTTCAGCCTGCAACAACATGCTCCTGAACCTTTAGTTGTTCTCCAAGGAGTGCTGAAGAAAATGAGGTTTCCGGTATACCTGCAAGATATTACGACAATGACCGCCTTACGCAGAGATGGACATCCTTCAGTGTATAGGAAGGCAACCAGCCAAGATGAGAAACAAAAAATAGGCCATTCATCTGATTGCAGTCATTGGTGCCTCCCTGGTGTGCCTGATATATGGAATGAGATGTTGAGTGCGCTGCTTTGA
Protein sequence

>Msa0158860

MATPMTHLLSFFLLFSIFHCTLSLLDPQDDVGFLVEPDDVNIVQNNRESSKRCDFSVGKWVYDESYPLYDPNCPYLSTAVTCQKNGRPDFDYEKWKWKPSGCSIPRFDALRFLGKMRRKRIMLVGDSIMRNQWESLVCLVQGVIPTGRKRVTYHGPAMAFHSMDFETSIEFLWAPLLVELKKSPENKRVLHLDLIEENAKYWRGVDILVFDSAQWWTHSDKTSSWDYYKEGNNLIKNMTLMVAYQKGLSTWARWVDLNLNPQKTKVIFRSMSPRHNRENGWKCDNQKQPLPFFSLQQHAPEPLVVLQGVLKKMRFPVYLQDITTMTALRRDGHPSVYRKATSQDEKQKIGHSSDCSHWCLPGVPDIWNEMLSALL*