AlfalfaGEDB | Msa0298870

M. sativa cultivar zhongmu-4 / Msa0298870

KEGG: K02978
GO: GO:0000028 GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005840 GO:0005886 GO:0006412 GO:0006518 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0015935 GO:0016020 GO:0016043 GO:0019538 GO:0022607 GO:0022613 GO:0022618 GO:0022626 GO:0022627 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0042254 GO:0042255 GO:0042274 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0065003 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0071944 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF01667.20

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0298870	EOA24972.1	97.674	86	2	0	1	86	30	115	9.56e-57	180

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0298870	sp\|Q9M2F1\|RS272_ARATH	96.512	86	3	0	1	86	1	86	1.83e-58	176

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0027590	Msa0298870	0.810699	1.012499e-50	-8.615850e-47
Msa0042480	Msa0298870	0.839190	1.837619e-57	-8.615850e-47
Msa0072570	Msa0298870	0.862071	6.571097e-64	-8.615850e-47
Msa0075350	Msa0298870	0.808502	3.001056e-50	-8.615850e-47
Msa0079590	Msa0298870	0.830294	3.183781e-55	-8.615850e-47
Msa0114570	Msa0298870	0.807366	5.235319e-50	-8.615850e-47
Msa0138890	Msa0298870	0.810456	1.142663e-50	-8.615850e-47
Msa0192750	Msa0298870	0.840549	8.132931e-58	-8.615850e-47
Msa0210360	Msa0298870	0.806440	8.216414e-50	-8.615850e-47
Msa0229150	Msa0298870	0.801166	1.023686e-48	-8.615850e-47
Msa0297830	Msa0298870	0.833767	4.414407e-56	-8.615850e-47
Msa0298870	Msa0301220	0.808995	2.355116e-50	-8.615850e-47
Msa0298870	Msa0335840	0.838059	3.600609e-57	-8.615850e-47
Msa0298870	Msa0369410	0.801715	7.900648e-49	-8.615850e-47
Msa0298870	Msa0471880	0.823179	1.592531e-53	-8.615850e-47
Msa0298870	Msa0540480	0.804806	1.810447e-49	-8.615850e-47
Msa0298870	Msa0546030	0.822160	2.748775e-53	-8.615850e-47
Msa0298870	Msa0550420	0.842991	1.843569e-58	-8.615850e-47
Msa0298870	Msa0582320	0.807629	4.603520e-50	-8.615850e-47
Msa0298870	Msa0584030	0.809783	1.595318e-50	-8.615850e-47
Msa0298870	Msa0643820	0.803623	3.191352e-49	-8.615850e-47
Msa0298870	Msa0705800	0.874862	4.847555e-68	-8.615850e-47
Msa0298870	Msa0712610	0.837336	5.519797e-57	-8.615850e-47
Msa0298870	Msa0781080	0.803921	2.766917e-49	-8.615850e-47
Msa0298870	Msa0788380	0.841020	6.120205e-58	-8.615850e-47
Msa0298870	Msa0876170	0.801367	9.309401e-49	-8.615850e-47
Msa0298870	Msa0915540	0.816031	6.818476e-52	-8.615850e-47
Msa0298870	Msa1037290	0.803478	3.419875e-49	-8.615850e-47
Msa0298870	Msa1040160	0.812544	4.019914e-51	-8.615850e-47
Msa0298870	Msa1041680	0.848956	4.403887e-60	-8.615850e-47
Msa0298870	Msa1058700	0.818657	1.748259e-52	-8.615850e-47
Msa0298870	Msa1080350	0.805225	1.479359e-49	-8.615850e-47
Msa0298870	Msa1083370	0.816986	4.167731e-52	-8.615850e-47
Msa0298870	Msa1126520	0.826016	3.418309e-54	-8.615850e-47
Msa0298870	Msa1166570	0.849888	2.421585e-60	-8.615850e-47
Msa0298870	Msa1167550	0.814686	1.357708e-51	-8.615850e-47
Msa0298870	Msa1186030	0.844562	7.002730e-59	-8.615850e-47
Msa0298870	Msa1218170	0.811554	6.607022e-51	-8.615850e-47
Msa0298870	Msa1240270	0.807298	5.412029e-50	-8.615850e-47
Msa0298870	Msa1311400	0.811861	5.664408e-51	-8.615850e-47
Msa0298870	Msa1356790	0.843224	1.598027e-58	-8.615850e-47
Msa0298870	Msa1368920	0.803463	3.444633e-49	-8.615850e-47
Msa0298870	Msa1374240	0.832000	1.213354e-55	-8.615850e-47
Msa0298870	Msa1392590	0.820385	7.053508e-53	-8.615850e-47
Msa0298870	Msa1397100	0.823274	1.512656e-53	-8.615850e-47
Msa0298870	Msa1403910	0.860474	2.014911e-63	-8.615850e-47
Msa0298870	Msa1426370	0.803172	3.957258e-49	-8.615850e-47
Msa0298870	Msa1426490	0.824259	8.892189e-54	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0298870	MtrunA17_Chr2g0291901	100.000	86	0	1	86	1	86	1.27e-60	179
Msa0298870	MtrunA17_Chr1g0210581	100.000	86	0	1	86	1	86	1.27e-60	179
Msa0298870	MtrunA17_Chr2g0283551	98.837	86	1	1	86	1	86	3.27e-60	177

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0298870	AT3G61110.1	96.512	86	3	1	86	1	86	1.86e-59	176
Msa0298870	AT2G45710.1	95.238	84	4	1	84	1	84	2.16e-56	168
Msa0298870	AT5G47930.1	96.429	84	3	1	84	1	84	4.81e-56	167
Msa0298870	AT3G61111.1	83.721	86	14	1	86	1	86	2.10e-49	150
Msa0298870	AT3G61111.2	80.952	42	8	1	42	1	42	1.05e-18	73.6

Find 41 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGGGTTGTCTTGTGGTTTA+TGG	0.128052	2_3:-53069448	Msa0298870:three_prime_UTR
TAACAGAAGGGTGCTCTTTC+AGG	0.162349	2_3:-53069586	Msa0298870:CDS
GTTGAAAGAATTTGAGTAAA+TGG	0.203742	2_3:-53069413	Msa0298870:three_prime_UTR
CATTTGCCTTAATTTATATC+TGG	0.266797	2_3:-53069362	Msa0298870:three_prime_UTR
ATCTCCTAATTCATTCTTCA+TGG	0.272640	2_3:-53070796	Msa0298870:intron
AAGGCTTAAAATTCAAAATA+TGG	0.294934	2_3:+53069479	None:intergenic
CACATCCGAACGGCGCAAAA+TGG	0.295168	2_3:-53070997	Msa0298870:intron
ATTACCATGAAGAATGAATT+AGG	0.300283	2_3:+53070792	None:intergenic
TACTGTCAACCAACTGTATA+AGG	0.333074	2_3:+53069388	None:intergenic
TGCCACAAACCACAACAGTT+TGG	0.361750	2_3:+53069654	None:intergenic
GCCACAAACCACAACAGTTT+GGG	0.376970	2_3:+53069655	None:intergenic
AAATTGCCAGATATAAATTA+AGG	0.385774	2_3:+53069356	None:intergenic
ATCTATTTCCAGTATCATCT+AGG	0.397342	2_3:-53071035	Msa0298870:five_prime_UTR
TTCCTCTTCTCAAGCTCAGC+AGG	0.425943	2_3:+53070843	None:intergenic
TCCCAAACTGTTGTGGTTTG+TGG	0.441541	2_3:-53069656	Msa0298870:CDS
GAGTAAACCCTAGATGATAC+TGG	0.452039	2_3:+53071027	None:intergenic
CTGGAAATAGATGGAGGCGC+TGG	0.461288	2_3:+53071046	None:intergenic
AGGGTGCTCTTTCAGGAAGA+AGG	0.484999	2_3:-53069579	Msa0298870:CDS
GTTGGAACTGGGTTGTCTTG+TGG	0.492314	2_3:-53069455	Msa0298870:three_prime_UTR
CTCTTCTCAAGCTCAGCAGG+TGG	0.497693	2_3:+53070846	None:intergenic
ATTTACTCAAATTCTTTCAA+CGG	0.511958	2_3:+53069415	None:intergenic
GGGTGCTCTTTCAGGAAGAA+GGG	0.516668	2_3:-53069578	Msa0298870:CDS
TCTATTTCCAGTATCATCTA+GGG	0.519395	2_3:-53071034	Msa0298870:five_prime_UTR
TTTAAGCCTTTGTTGGAACT+GGG	0.522619	2_3:-53069466	Msa0298870:three_prime_UTR
CAGTCACTCCCAAACTGTTG+TGG	0.529861	2_3:-53069663	Msa0298870:CDS
TAGATGATACTGGAAATAGA+TGG	0.534051	2_3:+53071037	None:intergenic
AGGCGCTGGGTGTATGAAGC+TGG	0.547200	2_3:+53071060	None:intergenic
TGGAAATAGATGGAGGCGCT+GGG	0.549766	2_3:+53071047	None:intergenic
ACGGTGTTGTGCCAACCTAC+CGG	0.557936	2_3:-53069623	Msa0298870:CDS
TAAATGGTACCTTATACAGT+TGG	0.560498	2_3:-53069397	Msa0298870:three_prime_UTR
GGAAGAAGGGAGATTAGTGA+TGG	0.567552	2_3:-53069565	Msa0298870:exon
CACCTGCTGAGCTTGAGAAG+AGG	0.575097	2_3:-53070845	Msa0298870:CDS
GGTTTGTGGCAACTGTCAAA+CGG	0.587352	2_3:-53069642	Msa0298870:CDS
CGCCGTTCGGATGTGTGATG+AGG	0.595230	2_3:+53071005	None:intergenic
TTGTAGGATGTCAAATGTCA+AGG	0.613415	2_3:-53070679	Msa0298870:intron
GGAAAAGCAAGATTAACAGA+AGG	0.617950	2_3:-53069599	Msa0298870:CDS
AGACAACCCAGTTCCAACAA+AGG	0.626981	2_3:+53069460	None:intergenic
GAAAAGCAAGATTAACAGAA+GGG	0.629527	2_3:-53069598	Msa0298870:CDS
CTCCTCATCACACATCCGAA+CGG	0.637905	2_3:-53071007	Msa0298870:five_prime_UTR
ATGATACTGGAAATAGATGG+AGG	0.666456	2_3:+53071040	None:intergenic
GTGTTGTGCCAACCTACCGG+TGG	0.670264	2_3:-53069620	Msa0298870:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATCTTATTTTTTATTCTTTT+TGG	-	chr2_3:53069671-53069690	Msa0298870:CDS	10.0%
!!!	TTATGATTATAAAACTTTTA+AGG	-	chr2_3:53070170-53070189	Msa0298870:intron	10.0%
!!!	TATGATTATAAAACTTTTAA+GGG	-	chr2_3:53070171-53070190	Msa0298870:intron	10.0%
!!!	AATGAAAATTTCAATTTTAA+AGG	+	chr2_3:53070214-53070233	None:intergenic	10.0%
!!!	ATGATTATAAAATTTTCTTA+GGG	-	chr2_3:53070320-53070339	Msa0298870:intron	10.0%
!!!	TTTGTTTTTTAACAAATAAT+AGG	-	chr2_3:53070591-53070610	Msa0298870:intron	10.0%
!!!	TTGTTTTTTAACAAATAATA+GGG	-	chr2_3:53070592-53070611	Msa0298870:intron	10.0%
!!!	ATCTTATTTTTTATTCTTTT+TGG	-	chr2_3:53069671-53069690	Msa0298870:CDS	10.0%
!!!	TTATGATTATAAAACTTTTA+AGG	-	chr2_3:53070170-53070189	Msa0298870:intron	10.0%
!!!	TATGATTATAAAACTTTTAA+GGG	-	chr2_3:53070171-53070190	Msa0298870:intron	10.0%
!!!	AATGAAAATTTCAATTTTAA+AGG	+	chr2_3:53070214-53070233	None:intergenic	10.0%
!!!	ATGATTATAAAATTTTCTTA+GGG	-	chr2_3:53070320-53070339	Msa0298870:intron	10.0%
!!!	TTTGTTTTTTAACAAATAAT+AGG	-	chr2_3:53070591-53070610	Msa0298870:intron	10.0%
!!!	TTGTTTTTTAACAAATAATA+GGG	-	chr2_3:53070592-53070611	Msa0298870:intron	10.0%
!!!	ATATATTTTCTATTCAAACT+TGG	+	chr2_3:53070251-53070270	None:intergenic	15.0%
!!!	CATGATTATAAAATTTTCTT+AGG	-	chr2_3:53070319-53070338	Msa0298870:intron	15.0%
!!!	ATTTGTTTTAGTGAAATTTT+GGG	-	chr2_3:53070856-53070875	Msa0298870:CDS	15.0%
!!!	ATATATTTTCTATTCAAACT+TGG	+	chr2_3:53070251-53070270	None:intergenic	15.0%
!!!	CATGATTATAAAATTTTCTT+AGG	-	chr2_3:53070319-53070338	Msa0298870:intron	15.0%
!!!	ATTTGTTTTAGTGAAATTTT+GGG	-	chr2_3:53070856-53070875	Msa0298870:CDS	15.0%
!!	TGTATTTGACAATAAATCTA+AGG	+	chr2_3:53070015-53070034	None:intergenic	20.0%
!!	AATGTAAAGTGATTTATGTT+CGG	+	chr2_3:53070055-53070074	None:intergenic	20.0%
!!!	CAATTTTGTAGAATTGATTT+TGG	+	chr2_3:53070091-53070110	None:intergenic	20.0%
!!!	TGTTTTATATTTCCCATTTA+CGG	-	chr2_3:53070272-53070291	Msa0298870:intron	20.0%
!!	ACAAGTGAATCAAATAAAAA+TGG	+	chr2_3:53070657-53070676	None:intergenic	20.0%
!!	GTTCATATTTCTTCATTTAT+TGG	-	chr2_3:53070675-53070694	Msa0298870:CDS	20.0%
!!!	GATTTGTTTTAGTGAAATTT+TGG	-	chr2_3:53070855-53070874	Msa0298870:CDS	20.0%
!!	AAGGCTTAAAATTCAAAATA+TGG	+	chr2_3:53070903-53070922	None:intergenic	20.0%
!!	ATTTACTCAAATTCTTTCAA+CGG	+	chr2_3:53070967-53070986	None:intergenic	20.0%
!!	AAATTGCCAGATATAAATTA+AGG	+	chr2_3:53071026-53071045	None:intergenic	20.0%
!!	TGTATTTGACAATAAATCTA+AGG	+	chr2_3:53070015-53070034	None:intergenic	20.0%
!!	AATGTAAAGTGATTTATGTT+CGG	+	chr2_3:53070055-53070074	None:intergenic	20.0%
!!!	CAATTTTGTAGAATTGATTT+TGG	+	chr2_3:53070091-53070110	None:intergenic	20.0%
!!!	TGTTTTATATTTCCCATTTA+CGG	-	chr2_3:53070272-53070291	Msa0298870:intron	20.0%
!!	ACAAGTGAATCAAATAAAAA+TGG	+	chr2_3:53070657-53070676	None:intergenic	20.0%
!!	GTTCATATTTCTTCATTTAT+TGG	-	chr2_3:53070675-53070694	Msa0298870:CDS	20.0%
!!!	GATTTGTTTTAGTGAAATTT+TGG	-	chr2_3:53070855-53070874	Msa0298870:CDS	20.0%
!!	AAGGCTTAAAATTCAAAATA+TGG	+	chr2_3:53070903-53070922	None:intergenic	20.0%
!!	ATTTACTCAAATTCTTTCAA+CGG	+	chr2_3:53070967-53070986	None:intergenic	20.0%
!!	AAATTGCCAGATATAAATTA+AGG	+	chr2_3:53071026-53071045	None:intergenic	20.0%
!	CAAAACAGAAAAATCAGAAT+CGG	+	chr2_3:53069464-53069483	None:intergenic	25.0%
!	ATTACCATGAAGAATGAATT+AGG	+	chr2_3:53069590-53069609	None:intergenic	25.0%
!!!	TTCTTTTTGGATTTGTTTGT+AGG	-	chr2_3:53069684-53069703	Msa0298870:intron	25.0%
!	CGTTGTTATAGATATAACAT+AGG	-	chr2_3:53069762-53069781	Msa0298870:intron	25.0%
!	AGATATAACATAGGCATAAT+TGG	-	chr2_3:53069771-53069790	Msa0298870:intron	25.0%
!!!	GTCAAATCACTTTTGTTATA+TGG	+	chr2_3:53069847-53069866	None:intergenic	25.0%
!!	TATGCAAGGAAAATCAATTT+TGG	-	chr2_3:53069914-53069933	Msa0298870:intron	25.0%
!!!	ACTTTTAAGGGTAATTATGA+TGG	-	chr2_3:53070183-53070202	Msa0298870:intron	25.0%
!!!	GTGAAAATGTCAGTTTTAAA+AGG	+	chr2_3:53070363-53070382	None:intergenic	25.0%
!	ACTAAAATCTAAGTTTCAGA+TGG	-	chr2_3:53070456-53070475	Msa0298870:intron	25.0%
!	AACTATAACCATACAAAAAC+AGG	+	chr2_3:53070519-53070538	None:intergenic	25.0%
!!!	TAGTGAAATTTTGGGTTATT+TGG	-	chr2_3:53070864-53070883	Msa0298870:CDS	25.0%
!!	TTTGAATTTTAAGCCTTTGT+TGG	-	chr2_3:53070906-53070925	Msa0298870:intron	25.0%
!	GTTGAAAGAATTTGAGTAAA+TGG	-	chr2_3:53070966-53070985	Msa0298870:intron	25.0%
!	CATTTGCCTTAATTTATATC+TGG	-	chr2_3:53071017-53071036	Msa0298870:five_prime_UTR	25.0%
!	CAAAACAGAAAAATCAGAAT+CGG	+	chr2_3:53069464-53069483	None:intergenic	25.0%
!	ATTACCATGAAGAATGAATT+AGG	+	chr2_3:53069590-53069609	None:intergenic	25.0%
!!!	TTCTTTTTGGATTTGTTTGT+AGG	-	chr2_3:53069684-53069703	Msa0298870:intron	25.0%
!	CGTTGTTATAGATATAACAT+AGG	-	chr2_3:53069762-53069781	Msa0298870:intron	25.0%
!	AGATATAACATAGGCATAAT+TGG	-	chr2_3:53069771-53069790	Msa0298870:intron	25.0%
!!!	GTCAAATCACTTTTGTTATA+TGG	+	chr2_3:53069847-53069866	None:intergenic	25.0%
!!	TATGCAAGGAAAATCAATTT+TGG	-	chr2_3:53069914-53069933	Msa0298870:intron	25.0%
!!!	ACTTTTAAGGGTAATTATGA+TGG	-	chr2_3:53070183-53070202	Msa0298870:intron	25.0%
!!!	GTGAAAATGTCAGTTTTAAA+AGG	+	chr2_3:53070363-53070382	None:intergenic	25.0%
!	ACTAAAATCTAAGTTTCAGA+TGG	-	chr2_3:53070456-53070475	Msa0298870:intron	25.0%
!	AACTATAACCATACAAAAAC+AGG	+	chr2_3:53070519-53070538	None:intergenic	25.0%
!!!	TAGTGAAATTTTGGGTTATT+TGG	-	chr2_3:53070864-53070883	Msa0298870:CDS	25.0%
!!	TTTGAATTTTAAGCCTTTGT+TGG	-	chr2_3:53070906-53070925	Msa0298870:intron	25.0%
!	GTTGAAAGAATTTGAGTAAA+TGG	-	chr2_3:53070966-53070985	Msa0298870:intron	25.0%
!	CATTTGCCTTAATTTATATC+TGG	-	chr2_3:53071017-53071036	Msa0298870:five_prime_UTR	25.0%
	TAGATGATACTGGAAATAGA+TGG	+	chr2_3:53069345-53069364	None:intergenic	30.0%
	ATCTATTTCCAGTATCATCT+AGG	-	chr2_3:53069344-53069363	Msa0298870:three_prime_UTR	30.0%
	TCTATTTCCAGTATCATCTA+GGG	-	chr2_3:53069345-53069364	Msa0298870:three_prime_UTR	30.0%
!!!	TTGATTTTGATTTCGCTTTC+AGG	-	chr2_3:53069481-53069500	Msa0298870:three_prime_UTR	30.0%
	ATCTCCTAATTCATTCTTCA+TGG	-	chr2_3:53069583-53069602	Msa0298870:CDS	30.0%
!!	ACGAGCAAATAAGTTTTGAT+AGG	+	chr2_3:53069745-53069764	None:intergenic	30.0%
	TAACATAGGCATAATTGGAA+AGG	-	chr2_3:53069776-53069795	Msa0298870:intron	30.0%
	AACATAGGCATAATTGGAAA+GGG	-	chr2_3:53069777-53069796	Msa0298870:intron	30.0%
	GCTGTAATATCATTTATGCA+AGG	-	chr2_3:53069900-53069919	Msa0298870:intron	30.0%
!	CAGCTTGAATTTGTAGTTTT+TGG	-	chr2_3:53069970-53069989	Msa0298870:intron	30.0%
!!!	TAGTTTTTGGCTTCTACAAA+TGG	-	chr2_3:53069983-53070002	Msa0298870:intron	30.0%
!	AAACTGACATTTTCACTTCT+TGG	-	chr2_3:53070367-53070386	Msa0298870:intron	30.0%
!!!	TGTGTAGTTTTGTGATTTGT+AGG	-	chr2_3:53070425-53070444	Msa0298870:intron	30.0%
!!!	GTGTAGTTTTGTGATTTGTA+GGG	-	chr2_3:53070426-53070445	Msa0298870:intron	30.0%
	AAGATACTATTGAGTAGCTA+TGG	+	chr2_3:53070554-53070573	None:intergenic	30.0%
	GAAAAGCAAGATTAACAGAA+GGG	-	chr2_3:53070781-53070800	Msa0298870:intron	30.0%
	TAAATGGTACCTTATACAGT+TGG	-	chr2_3:53070982-53071001	Msa0298870:intron	30.0%
	TAGATGATACTGGAAATAGA+TGG	+	chr2_3:53069345-53069364	None:intergenic	30.0%
	ATCTATTTCCAGTATCATCT+AGG	-	chr2_3:53069344-53069363	Msa0298870:three_prime_UTR	30.0%
	TCTATTTCCAGTATCATCTA+GGG	-	chr2_3:53069345-53069364	Msa0298870:three_prime_UTR	30.0%
!!!	TTGATTTTGATTTCGCTTTC+AGG	-	chr2_3:53069481-53069500	Msa0298870:three_prime_UTR	30.0%
	ATCTCCTAATTCATTCTTCA+TGG	-	chr2_3:53069583-53069602	Msa0298870:CDS	30.0%
!!	ACGAGCAAATAAGTTTTGAT+AGG	+	chr2_3:53069745-53069764	None:intergenic	30.0%
	TAACATAGGCATAATTGGAA+AGG	-	chr2_3:53069776-53069795	Msa0298870:intron	30.0%
	AACATAGGCATAATTGGAAA+GGG	-	chr2_3:53069777-53069796	Msa0298870:intron	30.0%
	GCTGTAATATCATTTATGCA+AGG	-	chr2_3:53069900-53069919	Msa0298870:intron	30.0%
!	CAGCTTGAATTTGTAGTTTT+TGG	-	chr2_3:53069970-53069989	Msa0298870:intron	30.0%
!!!	TAGTTTTTGGCTTCTACAAA+TGG	-	chr2_3:53069983-53070002	Msa0298870:intron	30.0%
!	AAACTGACATTTTCACTTCT+TGG	-	chr2_3:53070367-53070386	Msa0298870:intron	30.0%
!!!	TGTGTAGTTTTGTGATTTGT+AGG	-	chr2_3:53070425-53070444	Msa0298870:intron	30.0%
!!!	GTGTAGTTTTGTGATTTGTA+GGG	-	chr2_3:53070426-53070445	Msa0298870:intron	30.0%
	AAGATACTATTGAGTAGCTA+TGG	+	chr2_3:53070554-53070573	None:intergenic	30.0%
	GAAAAGCAAGATTAACAGAA+GGG	-	chr2_3:53070781-53070800	Msa0298870:intron	30.0%
	TAAATGGTACCTTATACAGT+TGG	-	chr2_3:53070982-53071001	Msa0298870:intron	30.0%
	ATGATACTGGAAATAGATGG+AGG	+	chr2_3:53069342-53069361	None:intergenic	35.0%
	TTGTAGGATGTCAAATGTCA+AGG	-	chr2_3:53069700-53069719	Msa0298870:intron	35.0%
	ACATAGGCATAATTGGAAAG+GGG	-	chr2_3:53069778-53069797	Msa0298870:intron	35.0%
!!	TTGTGTGTTTGGTTCTATAG+TGG	+	chr2_3:53070121-53070140	None:intergenic	35.0%
	AGAACCAAACACACAATAAG+TGG	-	chr2_3:53070125-53070144	Msa0298870:intron	35.0%
!	GTGGACTGTCGAATTAATAT+TGG	-	chr2_3:53070144-53070163	Msa0298870:intron	35.0%
	TCTACAGTTCTACCGTAAAT+GGG	+	chr2_3:53070287-53070306	None:intergenic	35.0%
	TTCTACAGTTCTACCGTAAA+TGG	+	chr2_3:53070288-53070307	None:intergenic	35.0%
	TGTCACTAAAAGAGGATACA+AGG	+	chr2_3:53070394-53070413	None:intergenic	35.0%
!	ATCCTCTTTTAGTGACAGAT+AGG	-	chr2_3:53070397-53070416	Msa0298870:intron	35.0%
	TTCCTATCTGTCACTAAAAG+AGG	+	chr2_3:53070402-53070421	None:intergenic	35.0%
	CCATACAAAAACAGGAAGTA+GGG	+	chr2_3:53070511-53070530	None:intergenic	35.0%
	ACCATACAAAAACAGGAAGT+AGG	+	chr2_3:53070512-53070531	None:intergenic	35.0%
!!	TGTTAATCTTGCTTTTCCAC+CGG	+	chr2_3:53070778-53070797	None:intergenic	35.0%
	GGAAAAGCAAGATTAACAGA+AGG	-	chr2_3:53070780-53070799	Msa0298870:intron	35.0%
!	TTTTAAGCCTTTGTTGGAAC+TGG	-	chr2_3:53070912-53070931	Msa0298870:intron	35.0%
	TTTAAGCCTTTGTTGGAACT+GGG	-	chr2_3:53070913-53070932	Msa0298870:intron	35.0%
	TACTGTCAACCAACTGTATA+AGG	+	chr2_3:53070994-53071013	None:intergenic	35.0%
	ATGATACTGGAAATAGATGG+AGG	+	chr2_3:53069342-53069361	None:intergenic	35.0%
	TTGTAGGATGTCAAATGTCA+AGG	-	chr2_3:53069700-53069719	Msa0298870:intron	35.0%
	ACATAGGCATAATTGGAAAG+GGG	-	chr2_3:53069778-53069797	Msa0298870:intron	35.0%
!!	TTGTGTGTTTGGTTCTATAG+TGG	+	chr2_3:53070121-53070140	None:intergenic	35.0%
	AGAACCAAACACACAATAAG+TGG	-	chr2_3:53070125-53070144	Msa0298870:intron	35.0%
!	GTGGACTGTCGAATTAATAT+TGG	-	chr2_3:53070144-53070163	Msa0298870:intron	35.0%
	TCTACAGTTCTACCGTAAAT+GGG	+	chr2_3:53070287-53070306	None:intergenic	35.0%
	TTCTACAGTTCTACCGTAAA+TGG	+	chr2_3:53070288-53070307	None:intergenic	35.0%
	TGTCACTAAAAGAGGATACA+AGG	+	chr2_3:53070394-53070413	None:intergenic	35.0%
!	ATCCTCTTTTAGTGACAGAT+AGG	-	chr2_3:53070397-53070416	Msa0298870:intron	35.0%
	TTCCTATCTGTCACTAAAAG+AGG	+	chr2_3:53070402-53070421	None:intergenic	35.0%
	CCATACAAAAACAGGAAGTA+GGG	+	chr2_3:53070511-53070530	None:intergenic	35.0%
	ACCATACAAAAACAGGAAGT+AGG	+	chr2_3:53070512-53070531	None:intergenic	35.0%
!!	TGTTAATCTTGCTTTTCCAC+CGG	+	chr2_3:53070778-53070797	None:intergenic	35.0%
	GGAAAAGCAAGATTAACAGA+AGG	-	chr2_3:53070780-53070799	Msa0298870:intron	35.0%
!	TTTTAAGCCTTTGTTGGAAC+TGG	-	chr2_3:53070912-53070931	Msa0298870:intron	35.0%
	TTTAAGCCTTTGTTGGAACT+GGG	-	chr2_3:53070913-53070932	Msa0298870:intron	35.0%
	TACTGTCAACCAACTGTATA+AGG	+	chr2_3:53070994-53071013	None:intergenic	35.0%
	GAGTAAACCCTAGATGATAC+TGG	+	chr2_3:53069355-53069374	None:intergenic	40.0%
!	ATTTACCATTTTGCGCCGTT+CGG	+	chr2_3:53069390-53069409	None:intergenic	40.0%
	TCAAAATCCACGAGTTCAAG+AGG	-	chr2_3:53069877-53069896	Msa0298870:intron	40.0%
	CAAAATCCACGAGTTCAAGA+GGG	-	chr2_3:53069878-53069897	Msa0298870:intron	40.0%
	CAGTCCACTTATTGTGTGTT+TGG	+	chr2_3:53070132-53070151	None:intergenic	40.0%
!!!	CCCTACTTCCTGTTTTTGTA+TGG	-	chr2_3:53070508-53070527	Msa0298870:intron	40.0%
	GAGTAAACCCTAGATGATAC+TGG	+	chr2_3:53069355-53069374	None:intergenic	40.0%
!	ATTTACCATTTTGCGCCGTT+CGG	+	chr2_3:53069390-53069409	None:intergenic	40.0%
	TCAAAATCCACGAGTTCAAG+AGG	-	chr2_3:53069877-53069896	Msa0298870:intron	40.0%
	CAAAATCCACGAGTTCAAGA+GGG	-	chr2_3:53069878-53069897	Msa0298870:intron	40.0%
	CAGTCCACTTATTGTGTGTT+TGG	+	chr2_3:53070132-53070151	None:intergenic	40.0%
!!!	CCCTACTTCCTGTTTTTGTA+TGG	-	chr2_3:53070508-53070527	Msa0298870:intron	40.0%
	TCCCAAACTGTTGTGGTTTG+TGG	-	chr2_3:53070723-53070742	Msa0298870:intron	45.0%
	GCCACAAACCACAACAGTTT+GGG	+	chr2_3:53070727-53070746	None:intergenic	45.0%
	TGCCACAAACCACAACAGTT+TGG	+	chr2_3:53070728-53070747	None:intergenic	45.0%
	GGTTTGTGGCAACTGTCAAA+CGG	-	chr2_3:53070737-53070756	Msa0298870:intron	45.0%
!!	AATCTTGCTTTTCCACCGGT+AGG	+	chr2_3:53070774-53070793	None:intergenic	45.0%
!!	TAACAGAAGGGTGCTCTTTC+AGG	-	chr2_3:53070793-53070812	Msa0298870:intron	45.0%
	GGAAGAAGGGAGATTAGTGA+TGG	-	chr2_3:53070814-53070833	Msa0298870:CDS	45.0%
	AGACAACCCAGTTCCAACAA+AGG	+	chr2_3:53070922-53070941	None:intergenic	45.0%
	CTGGGTTGTCTTGTGGTTTA+TGG	-	chr2_3:53070931-53070950	Msa0298870:intron	45.0%
	TCCCAAACTGTTGTGGTTTG+TGG	-	chr2_3:53070723-53070742	Msa0298870:intron	45.0%
	GCCACAAACCACAACAGTTT+GGG	+	chr2_3:53070727-53070746	None:intergenic	45.0%
	TGCCACAAACCACAACAGTT+TGG	+	chr2_3:53070728-53070747	None:intergenic	45.0%
	GGTTTGTGGCAACTGTCAAA+CGG	-	chr2_3:53070737-53070756	Msa0298870:intron	45.0%
!!	AATCTTGCTTTTCCACCGGT+AGG	+	chr2_3:53070774-53070793	None:intergenic	45.0%
!!	TAACAGAAGGGTGCTCTTTC+AGG	-	chr2_3:53070793-53070812	Msa0298870:intron	45.0%
	GGAAGAAGGGAGATTAGTGA+TGG	-	chr2_3:53070814-53070833	Msa0298870:CDS	45.0%
	AGACAACCCAGTTCCAACAA+AGG	+	chr2_3:53070922-53070941	None:intergenic	45.0%
	CTGGGTTGTCTTGTGGTTTA+TGG	-	chr2_3:53070931-53070950	Msa0298870:intron	45.0%
!!	TGGAAATAGATGGAGGCGCT+GGG	+	chr2_3:53069335-53069354	None:intergenic	50.0%
	CTCCTCATCACACATCCGAA+CGG	-	chr2_3:53069372-53069391	Msa0298870:three_prime_UTR	50.0%
	TTCCTCTTCTCAAGCTCAGC+AGG	+	chr2_3:53069539-53069558	None:intergenic	50.0%
	TTACAGCCCTCTTGAACTCG+TGG	+	chr2_3:53069887-53069906	None:intergenic	50.0%
	CAGTCACTCCCAAACTGTTG+TGG	-	chr2_3:53070716-53070735	Msa0298870:intron	50.0%
!!	TTGCTTTTCCACCGGTAGGT+TGG	+	chr2_3:53070770-53070789	None:intergenic	50.0%
!	AGGGTGCTCTTTCAGGAAGA+AGG	-	chr2_3:53070800-53070819	Msa0298870:CDS	50.0%
	GGGTGCTCTTTCAGGAAGAA+GGG	-	chr2_3:53070801-53070820	Msa0298870:CDS	50.0%
	GTTGGAACTGGGTTGTCTTG+TGG	-	chr2_3:53070924-53070943	Msa0298870:intron	50.0%
!!	TGGAAATAGATGGAGGCGCT+GGG	+	chr2_3:53069335-53069354	None:intergenic	50.0%
	CTCCTCATCACACATCCGAA+CGG	-	chr2_3:53069372-53069391	Msa0298870:three_prime_UTR	50.0%
	TTCCTCTTCTCAAGCTCAGC+AGG	+	chr2_3:53069539-53069558	None:intergenic	50.0%
	TTACAGCCCTCTTGAACTCG+TGG	+	chr2_3:53069887-53069906	None:intergenic	50.0%
	CAGTCACTCCCAAACTGTTG+TGG	-	chr2_3:53070716-53070735	Msa0298870:intron	50.0%
!!	TTGCTTTTCCACCGGTAGGT+TGG	+	chr2_3:53070770-53070789	None:intergenic	50.0%
!	AGGGTGCTCTTTCAGGAAGA+AGG	-	chr2_3:53070800-53070819	Msa0298870:CDS	50.0%
	GGGTGCTCTTTCAGGAAGAA+GGG	-	chr2_3:53070801-53070820	Msa0298870:CDS	50.0%
	GTTGGAACTGGGTTGTCTTG+TGG	-	chr2_3:53070924-53070943	Msa0298870:intron	50.0%
!	CTGGAAATAGATGGAGGCGC+TGG	+	chr2_3:53069336-53069355	None:intergenic	55.0%
	CACATCCGAACGGCGCAAAA+TGG	-	chr2_3:53069382-53069401	Msa0298870:three_prime_UTR	55.0%
	CTCTTCTCAAGCTCAGCAGG+TGG	+	chr2_3:53069536-53069555	None:intergenic	55.0%
	CACCTGCTGAGCTTGAGAAG+AGG	-	chr2_3:53069534-53069553	Msa0298870:three_prime_UTR	55.0%
!!	ACGGTGTTGTGCCAACCTAC+CGG	-	chr2_3:53070756-53070775	Msa0298870:intron	55.0%
!	CTGGAAATAGATGGAGGCGC+TGG	+	chr2_3:53069336-53069355	None:intergenic	55.0%
	CACATCCGAACGGCGCAAAA+TGG	-	chr2_3:53069382-53069401	Msa0298870:three_prime_UTR	55.0%
	CTCTTCTCAAGCTCAGCAGG+TGG	+	chr2_3:53069536-53069555	None:intergenic	55.0%
	CACCTGCTGAGCTTGAGAAG+AGG	-	chr2_3:53069534-53069553	Msa0298870:three_prime_UTR	55.0%
!!	ACGGTGTTGTGCCAACCTAC+CGG	-	chr2_3:53070756-53070775	Msa0298870:intron	55.0%
!!	AGGCGCTGGGTGTATGAAGC+TGG	+	chr2_3:53069322-53069341	None:intergenic	60.0%
	CGCCGTTCGGATGTGTGATG+AGG	+	chr2_3:53069377-53069396	None:intergenic	60.0%
	GTGTTGTGCCAACCTACCGG+TGG	-	chr2_3:53070759-53070778	Msa0298870:intron	60.0%
!!	AGGCGCTGGGTGTATGAAGC+TGG	+	chr2_3:53069322-53069341	None:intergenic	60.0%
	CGCCGTTCGGATGTGTGATG+AGG	+	chr2_3:53069377-53069396	None:intergenic	60.0%
	GTGTTGTGCCAACCTACCGG+TGG	-	chr2_3:53070759-53070778	Msa0298870:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	53069315	53071086	53069315	ID=Msa0298870;Name=Msa0298870
chr2_3	mRNA	53069315	53071086	53069315	ID=Msa0298870-mRNA-1;Parent=Msa0298870;Name=Msa0298870-mRNA-1;_AED=0.00;_eAED=0.00;_QI=86\|1\|1\|1\|1\|1\|4\|256\|86
chr2_3	exon	53069315	53069697	53069315	ID=Msa0298870-mRNA-1:exon:17000;Parent=Msa0298870-mRNA-1
chr2_3	exon	53070667	53070695	53070667	ID=Msa0298870-mRNA-1:exon:16999;Parent=Msa0298870-mRNA-1
chr2_3	exon	53070797	53070898	53070797	ID=Msa0298870-mRNA-1:exon:16998;Parent=Msa0298870-mRNA-1
chr2_3	exon	53070998	53071086	53070998	ID=Msa0298870-mRNA-1:exon:16997;Parent=Msa0298870-mRNA-1
chr2_3	five_prime_UTR	53071001	53071086	53071001	ID=Msa0298870-mRNA-1:five_prime_utr;Parent=Msa0298870-mRNA-1
chr2_3	CDS	53070998	53071000	53070998	ID=Msa0298870-mRNA-1:cds;Parent=Msa0298870-mRNA-1
chr2_3	CDS	53070797	53070898	53070797	ID=Msa0298870-mRNA-1:cds;Parent=Msa0298870-mRNA-1
chr2_3	CDS	53070667	53070695	53070667	ID=Msa0298870-mRNA-1:cds;Parent=Msa0298870-mRNA-1
chr2_3	CDS	53069571	53069697	53069571	ID=Msa0298870-mRNA-1:cds;Parent=Msa0298870-mRNA-1
chr2_3	three_prime_UTR	53069315	53069570	53069315	ID=Msa0298870-mRNA-1:three_prime_utr;Parent=Msa0298870-mRNA-1

Gene Sequence	>Msa0298870 ATGGTTCTTCAAAATGATATTGATTTGTTAAATCCACCTGCTGAGCTTGAGAAGAGGAAGCATAAGCTCAAGCGACTTGTTCAATCTCCTAATTCATTCTTCATGGATGTCAAATGTCAAGGTTGCTTCAATATAACAACTGTATTCAGTCACTCCCAAACTGTTGTGGTTTGTGGCAACTGTCAAACGGTGTTGTGCCAACCTACCGGTGGAAAAGCAAGATTAACAGAAGGGTGCTCTTTCAGGAAGAAGGGAGATTAG
Protein sequence	>Msa0298870 MVLQNDIDLLNPPAELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVVCGNCQTVLCQPTGGKARLTEGCSFRKKGD*