AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0516110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0516110 MtrunA17_Chr3g0126611 96.134 388 15 0 1 388 1 388 0.0 778
Msa0516110 MtrunA17_Chr8g0347101 40.449 356 197 9 26 376 14 359 2.05e-79 248
Msa0516110 MtrunA17_Chr8g0388231 38.655 357 198 10 37 376 30 382 1.46e-70 227
Msa0516110 MtrunA17_Chr8g0388201 35.904 376 208 12 32 381 29 397 9.23e-62 204
Msa0516110 MtrunA17_Chr5g0396601 31.461 356 212 12 44 376 49 395 2.11e-42 152
Msa0516110 MtrunA17_Chr5g0396611 31.405 363 213 13 39 376 34 385 4.00e-40 146
Msa0516110 MtrunA17_Chr3g0081611 43.478 138 70 2 245 376 18 153 1.08e-34 126
Msa0516110 MtrunA17_Chr5g0396591 32.819 259 161 7 125 376 33 285 3.93e-30 117
Msa0516110 MtrunA17_Chr7g0275601 37.195 164 83 6 221 376 2 153 1.36e-25 101
Msa0516110 MtrunA17_Chr1g0160671 30.943 265 155 10 14 267 9 256 4.67e-25 102
Msa0516110 MtrunA17_Chr1g0158771 33.439 314 166 16 8 315 8 284 2.85e-24 104
Msa0516110 MtrunA17_Chr1g0160771 31.902 326 164 20 8 315 11 296 8.84e-24 103
Msa0516110 MtrunA17_Chr1g0160731 31.206 282 158 14 44 315 37 292 2.02e-23 102
Msa0516110 MtrunA17_Chr1g0160581 29.338 317 190 14 13 315 4 300 3.91e-22 98.2
Msa0516110 MtrunA17_Chr8g0348151 37.241 145 73 4 245 376 20 159 9.93e-19 84.0
Msa0516110 MtrunA17_Chr4g0037001 29.496 278 159 13 7 265 6 265 2.13e-18 87.0
Msa0516110 MtrunA17_Chr1g0158531 28.090 267 158 11 45 299 37 281 3.76e-18 86.3
Msa0516110 MtrunA17_Chr1g0158051 31.884 207 124 8 31 228 25 223 6.84e-18 85.5
Msa0516110 MtrunA17_Chr7g0248831 33.803 142 84 1 245 376 12 153 1.04e-17 80.5
Msa0516110 MtrunA17_Chr1g0158651 27.206 272 156 12 35 293 50 292 5.98e-16 79.7
Msa0516110 MtrunA17_Chr1g0158311 24.809 262 170 10 45 299 38 279 1.02e-15 79.0
Msa0516110 MtrunA17_Chr1g0158181 28.696 230 140 8 38 255 56 273 2.73e-15 77.4
Msa0516110 MtrunA17_Chr1g0177041 44.737 76 39 2 303 376 74 148 4.34e-15 72.8
Msa0516110 MtrunA17_Chr5g0399521 31.183 186 111 8 43 220 27 203 8.23e-15 73.6
Msa0516110 MtrunA17_Chr1g0157801 28.641 206 127 7 31 228 24 217 9.01e-15 75.9
Msa0516110 MtrunA17_Chr1g0158621 40.000 110 55 5 35 135 33 140 2.83e-14 70.1
Msa0516110 MtrunA17_Chr5g0406031 31.897 116 74 1 263 373 116 231 5.29e-14 70.9
Msa0516110 MtrunA17_Chr1g0157971 24.839 310 207 10 31 329 26 320 6.36e-14 73.2
Msa0516110 MtrunA17_Chr8g0383871 40.845 71 41 1 303 373 112 181 6.61e-14 70.5
Msa0516110 MtrunA17_Chr3g0128771 41.096 73 43 0 301 373 89 161 7.59e-14 69.3
Msa0516110 MtrunA17_Chr8g0383921 40.845 71 41 1 303 373 111 180 9.52e-14 69.7
Msa0516110 MtrunA17_Chr1g0198201 36.585 82 51 1 303 384 61 141 1.07e-13 70.1
Msa0516110 MtrunA17_Chr1g0158101 28.708 209 131 8 38 235 34 235 1.61e-13 72.0
Msa0516110 MtrunA17_Chr8g0383881 39.437 71 42 1 303 373 103 172 5.76e-13 67.8
Msa0516110 MtrunA17_Chr8g0383941 37.333 75 46 1 299 373 103 176 6.08e-13 67.8
Msa0516110 MtrunA17_Chr5g0413841 39.189 74 43 1 302 375 115 186 6.38e-13 69.3
Msa0516110 MtrunA17_Chr8g0383861 39.437 71 42 1 303 373 113 182 8.11e-13 67.4
Msa0516110 MtrunA17_Chr4g0027761 42.105 76 39 1 298 373 95 165 1.37e-12 66.2
Msa0516110 MtrunA17_Chr7g0248841 39.241 79 38 2 305 373 68 146 1.39e-12 65.5
Msa0516110 MtrunA17_Chr1g0157781 26.693 251 163 9 28 266 21 262 1.60e-12 68.9
Msa0516110 MtrunA17_Chr6g0486651 43.056 72 39 1 302 373 92 161 1.98e-12 68.2
Msa0516110 MtrunA17_Chr5g0409211 46.429 56 30 0 320 375 83 138 2.07e-12 65.1
Msa0516110 MtrunA17_Chr3g0129671 37.500 72 44 1 302 373 108 178 2.22e-12 66.2
Msa0516110 MtrunA17_Chr7g0273051 39.189 74 44 1 303 375 91 164 2.44e-12 67.8
Msa0516110 MtrunA17_Chr5g0407431 26.619 139 89 2 236 373 21 147 2.75e-12 65.9
Msa0516110 MtrunA17_Chr3g0132491 28.125 128 82 2 247 373 60 178 3.15e-12 67.4
Msa0516110 MtrunA17_Chr7g0244851 40.506 79 39 2 300 375 78 151 4.69e-12 64.3
Msa0516110 MtrunA17_Chr8g0370171 40.278 72 43 0 303 374 70 141 5.01e-12 64.7
Msa0516110 MtrunA17_Chr3g0133001 36.111 72 44 1 302 373 129 198 5.48e-12 67.0
Msa0516110 MtrunA17_Chr5g0426881 32.759 116 68 3 260 373 78 185 5.66e-12 66.6
Msa0516110 MtrunA17_Chr8g0366561 30.769 130 80 2 250 375 63 186 8.81e-12 65.9
Msa0516110 MtrunA17_Chr5g0409201 45.000 60 27 1 316 375 84 137 9.25e-12 62.8
Msa0516110 MtrunA17_Chr8g0364881 37.838 74 44 1 302 375 67 138 1.09e-11 65.5
Msa0516110 MtrunA17_Chr1g0197031 27.660 141 96 3 238 376 11 147 1.21e-11 62.8
Msa0516110 MtrunA17_Chr1g0191601 37.838 74 45 1 303 375 98 171 1.34e-11 65.5
Msa0516110 MtrunA17_Chr8g0371291 37.500 72 39 1 302 373 157 222 1.53e-11 63.5
Msa0516110 MtrunA17_Chr5g0437361 31.481 108 68 1 268 375 46 147 1.66e-11 62.4
Msa0516110 MtrunA17_Chr7g0274721 33.721 86 54 1 289 374 54 136 1.83e-11 62.4
Msa0516110 MtrunA17_Chr2g0295571 38.095 84 50 2 299 382 89 170 1.99e-11 64.3
Msa0516110 MtrunA17_Chr5g0444141 33.028 109 69 2 268 374 157 263 2.21e-11 63.9
Msa0516110 MtrunA17_Chr5g0444161 29.565 115 77 2 265 377 119 231 2.35e-11 63.2
Msa0516110 MtrunA17_Chr5g0427431 35.897 78 47 2 296 373 128 202 2.57e-11 64.7
Msa0516110 MtrunA17_Chr6g0451161 35.897 78 48 1 298 375 128 203 2.74e-11 64.7
Msa0516110 MtrunA17_Chr2g0297011 31.618 136 81 3 238 373 21 144 2.95e-11 61.6
Msa0516110 MtrunA17_Chr7g0246761 30.000 120 80 3 255 373 16 132 3.00e-11 62.0
Msa0516110 MtrunA17_Chr7g0274741 36.986 73 45 1 303 374 70 142 3.66e-11 61.6
Msa0516110 MtrunA17_Chr4g0013561 34.444 90 50 2 292 375 68 154 3.83e-11 62.4
Msa0516110 MtrunA17_Chr4g0059281 28.244 131 87 2 249 374 47 175 3.91e-11 63.5
Msa0516110 MtrunA17_Chr1g0184171 29.412 136 86 5 246 375 43 174 5.22e-11 63.5
Msa0516110 MtrunA17_Chr5g0433861 30.370 135 86 5 259 386 31 164 6.27e-11 62.8
Msa0516110 MtrunA17_Chr8g0381731 34.940 83 50 2 293 373 136 216 6.50e-11 62.8
Msa0516110 MtrunA17_Chr2g0300331 56.818 44 19 0 331 374 169 212 7.52e-11 62.0
Msa0516110 MtrunA17_Chr4g0060981 33.333 78 49 1 296 373 142 216 7.54e-11 61.6
Msa0516110 MtrunA17_Chr7g0274731 46.000 50 27 0 325 374 108 157 7.88e-11 60.8
Msa0516110 MtrunA17_Chr4g0050041 36.364 88 52 2 289 374 110 195 9.47e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0516110 AT5G53110.1 47.578 351 174 9 35 379 32 378 5.97e-106 317
Msa0516110 AT2G46494.1 39.883 341 183 8 38 375 39 360 6.19e-71 227
Msa0516110 AT2G46495.1 35.328 351 213 9 31 376 24 365 2.23e-66 215
Msa0516110 AT2G46495.2 35.143 350 187 7 31 376 24 337 4.03e-65 211
Msa0516110 AT1G28040.1 38.127 299 166 7 88 375 7 297 4.91e-58 191
Msa0516110 AT2G25410.1 32.869 359 219 12 33 380 29 376 1.85e-56 189
Msa0516110 AT5G36001.1 31.073 354 178 9 31 383 28 316 2.38e-45 159
Msa0516110 AT5G07040.1 43.972 141 71 3 237 376 6 139 1.24e-32 120
Msa0516110 AT2G46493.1 31.707 205 109 4 175 375 5 182 2.99e-28 109
Msa0516110 AT5G36001.2 30.732 205 119 6 31 234 28 210 5.46e-25 102
Msa0516110 AT3G61550.1 37.107 159 75 3 243 376 23 181 4.16e-23 96.7
Msa0516110 AT2G35910.1 34.559 136 79 2 245 376 63 192 5.65e-18 82.4
Msa0516110 AT2G46160.1 36.601 153 73 4 245 376 34 183 8.43e-17 79.0
Msa0516110 AT2G46160.2 36.601 153 73 4 245 376 34 183 8.43e-17 79.0
Msa0516110 AT5G06490.1 30.323 155 83 4 245 376 22 174 7.46e-15 73.2
Msa0516110 AT5G66070.5 34.109 129 72 4 254 373 94 218 2.07e-14 72.4
Msa0516110 AT5G66070.1 34.109 129 72 4 254 373 94 218 2.07e-14 72.4
Msa0516110 AT4G09110.1 45.833 72 38 1 303 373 94 165 5.12e-14 72.4
Msa0516110 AT2G17730.1 31.624 117 74 2 263 373 122 238 5.97e-14 71.2
Msa0516110 AT2G42350.1 38.889 90 44 3 303 386 75 159 6.74e-14 70.9
Msa0516110 AT4G09120.1 45.205 73 39 1 303 374 94 166 9.57e-14 72.4
Msa0516110 AT1G14200.1 40.278 72 39 2 306 377 87 154 1.97e-13 68.6
Msa0516110 AT4G09130.1 43.836 73 40 1 303 374 91 163 2.08e-13 71.2
Msa0516110 AT1G35330.1 34.091 132 79 4 252 377 46 175 2.74e-13 70.9
Msa0516110 AT5G66070.2 39.726 73 40 1 301 373 174 242 3.63e-13 69.3
Msa0516110 AT4G35840.1 30.769 117 75 2 263 373 117 233 5.50e-13 68.6
Msa0516110 AT5G05810.1 37.500 72 43 1 302 373 119 188 7.59e-13 70.1
Msa0516110 AT1G49230.1 41.333 75 42 2 299 373 101 173 1.05e-12 67.4
Msa0516110 AT2G20030.1 43.056 72 39 1 302 373 97 166 1.51e-12 68.9
Msa0516110 AT1G49200.1 34.667 75 48 1 299 373 105 178 2.12e-12 66.6
Msa0516110 AT3G48030.2 37.975 79 46 2 298 375 97 173 2.21e-12 67.4
Msa0516110 AT1G18390.2 28.395 243 140 12 1 222 1 230 2.35e-12 68.9
Msa0516110 AT2G35000.1 37.634 93 54 3 284 373 86 177 3.30e-12 67.8
Msa0516110 AT4G28890.1 41.667 72 40 1 302 373 96 165 3.30e-12 68.2
Msa0516110 AT3G48030.1 37.975 79 46 2 298 375 175 251 3.61e-12 67.4
Msa0516110 AT3G10910.1 30.556 144 95 2 235 375 17 158 4.11e-12 64.7
Msa0516110 AT4G09100.1 39.189 74 44 1 303 375 56 129 4.59e-12 63.5
Msa0516110 AT5G17600.1 31.818 132 73 4 250 373 62 184 5.68e-12 67.0
Msa0516110 AT3G05200.1 38.356 73 44 1 303 374 99 171 7.30e-12 67.0
Msa0516110 AT5G27420.1 39.744 78 46 1 299 375 91 168 8.71e-12 66.6
Msa0516110 AT1G49210.1 36.620 71 44 1 303 373 108 177 1.17e-11 64.3
Msa0516110 AT2G27940.1 37.662 77 45 1 298 374 110 183 1.45e-11 64.3
Msa0516110 AT3G16720.1 37.838 74 43 1 301 374 92 162 1.49e-11 65.1
Msa0516110 AT1G04360.1 38.750 80 40 2 303 375 101 178 1.80e-11 65.5
Msa0516110 AT4G17905.2 36.842 76 48 0 299 374 149 224 2.04e-11 65.1
Msa0516110 AT3G18773.1 38.028 71 43 1 303 373 103 172 2.12e-11 63.5
Msa0516110 AT4G17905.1 36.842 76 48 0 299 374 123 198 2.42e-11 64.7
Msa0516110 AT5G05280.1 44.262 61 34 0 325 385 107 167 2.96e-11 62.4
Msa0516110 AT2G35420.1 41.667 72 40 1 302 373 76 145 3.15e-11 63.5
Msa0516110 AT4G40070.1 36.782 87 52 2 290 374 82 167 3.36e-11 64.3
Msa0516110 AT5G47610.1 41.270 63 36 1 313 375 90 151 3.60e-11 61.6
Msa0516110 AT3G14320.1 44.262 61 31 1 331 388 88 148 5.50e-11 62.0
Msa0516110 AT5G57750.1 32.051 78 44 2 300 373 92 164 7.86e-11 61.6
Msa0516110 AT2G34990.2 35.955 89 54 2 286 373 74 160 8.04e-11 63.2
Msa0516110 AT2G34990.1 35.955 89 54 2 286 373 52 138 8.18e-11 63.2
Msa0516110 AT1G76410.1 31.933 119 68 3 258 373 38 146 9.15e-11 61.2
Msa0516110 AT1G53010.1 37.500 80 45 2 303 377 97 176 9.52e-11 60.8
Msa0516110 AT1G49220.1 32.927 82 54 1 292 373 97 177 9.80e-11 62.0

Find 84 sgRNAs with CRISPR-Local

Find 300 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGGAGAAGTTCTACAAAT+TGG 0.237722 3_4:+67542548 None:intergenic
GAAACAAATTAGGATGAGTT+TGG 0.247580 3_4:+67544670 None:intergenic
TTTCCATGTAAGGTAGATTA+AGG 0.247749 3_4:+67544456 None:intergenic
TTGAGTGAGAGAAGTTGTTT+TGG 0.275761 3_4:+67544365 None:intergenic
GCCACTGCTTGTGTTTGATT+TGG 0.289245 3_4:+67544632 None:intergenic
TTTGGGTATGATTCAATTGT+TGG 0.299282 3_4:+67542737 None:intergenic
CCACTGCTTGTGTTTGATTT+GGG 0.306529 3_4:+67544633 None:intergenic
AGAGGAAAAGGAAACAAATT+AGG 0.345874 3_4:+67544660 None:intergenic
TGAAAGCAATGTTCACATTC+AGG 0.369028 3_4:+67542614 None:intergenic
CATGTCTTACCATTGGGTTT+TGG 0.369951 3_4:+67542686 None:intergenic
AGCTGGCACCCCTGCACATA+TGG 0.376864 3_4:+67542904 None:intergenic
AAACAAATTAGGATGAGTTT+GGG 0.376952 3_4:+67544671 None:intergenic
CTTGTCGTCTGTTTGTGATT+TGG 0.386576 3_4:-67544177 Msa0516110:CDS
TTGGTACCCATGGTTGGACT+TGG 0.389757 3_4:-67542832 Msa0516110:CDS
GGTAAATTGTTGATAGTAAA+CGG 0.390917 3_4:+67544319 None:intergenic
AAGGTAGATTAAGGAGTGTT+TGG 0.396216 3_4:+67544465 None:intergenic
CTTCTATATGGAATGGGAAT+CGG 0.405821 3_4:+67544552 None:intergenic
AATTAGGATGAGTTTGGGAT+TGG 0.407156 3_4:+67544676 None:intergenic
CGATTCCCATTCCATATAGA+AGG 0.408041 3_4:-67544551 Msa0516110:CDS
AGTGTCTAAGCATGTTTCTA+TGG 0.409320 3_4:+67544601 None:intergenic
CATGAATACACCAACACTTC+AGG 0.414069 3_4:-67542770 Msa0516110:CDS
TGATTGAACCGGCACGTTAA+CGG 0.417888 3_4:+67544148 None:intergenic
ATTAGGATGAGTTTGGGATT+GGG 0.421358 3_4:+67544677 None:intergenic
ATTGGACATGTCTTACCATT+GGG 0.427757 3_4:+67542680 None:intergenic
GAGGAGAAGTTCTACAAATT+GGG 0.432579 3_4:+67542549 None:intergenic
CTCGCTTGTGATTGTAACCT+TGG 0.444817 3_4:+67542527 None:intergenic
GCTTCATTTCACTATTAAGC+TGG 0.445778 3_4:-67542874 Msa0516110:CDS
TATTGGACATGTCTTACCAT+TGG 0.455747 3_4:+67542679 None:intergenic
TGCATAATACTTTGAACCCT+GGG 0.459124 3_4:+67544504 None:intergenic
TAATCACTAAGACAAGCTAT+TGG 0.462186 3_4:+67544248 None:intergenic
ATATATTCTGACAAGCATAT+TGG 0.471017 3_4:+67542662 None:intergenic
CACCCCTGCACATATGGCTA+TGG 0.474021 3_4:+67542910 None:intergenic
ATATGTAGCAAGTTCAGAAT+TGG 0.477062 3_4:-67542851 Msa0516110:CDS
GATTTGGGTTGAAGAGGAAA+AGG 0.480262 3_4:+67544648 None:intergenic
ATGCACAATGCATTGATGAA+TGG 0.486845 3_4:-67542592 Msa0516110:CDS
TGGAATGGGAATCGGATTAG+TGG 0.500897 3_4:+67544560 None:intergenic
ACTCCTTAATCTACCTTACA+TGG 0.500945 3_4:-67544459 Msa0516110:CDS
TGATAGTAAACGGCATCAAA+AGG 0.512565 3_4:+67544329 None:intergenic
TATGGCGTAACTTGCACCAC+GGG 0.514622 3_4:+67542928 None:intergenic
ATGGCGAATTCCATGACAAC+CGG 0.515784 3_4:+67544081 None:intergenic
GGTTGATACCGTTAACGTGC+CGG 0.519040 3_4:-67544156 Msa0516110:CDS
TTGCATAATACTTTGAACCC+TGG 0.524383 3_4:+67544503 None:intergenic
GCTTGTGATTGTAACCTTGG+AGG 0.525892 3_4:+67542530 None:intergenic
GAAATGAAGCACAACAAAGC+TGG 0.534895 3_4:+67542887 None:intergenic
GTACTATTATTACCTTGTCG+TGG 0.537383 3_4:+67543975 None:intergenic
TACGCCATAGCCATATGTGC+AGG 0.538697 3_4:-67542914 Msa0516110:CDS
CATCAAAAGGAGAGCTAGAG+AGG 0.546542 3_4:+67544342 None:intergenic
ACTTGGAATATCTTACCTCT+AGG 0.548359 3_4:-67544277 Msa0516110:CDS
TGGTACCCATGGTTGGACTT+GGG 0.548632 3_4:-67542831 Msa0516110:CDS
TTTGTAGAACTTCTCCTCCA+AGG 0.550552 3_4:-67542544 Msa0516110:CDS
TCAGAATTGGTACCCATGGT+TGG 0.553561 3_4:-67542838 Msa0516110:CDS
AAGTTCAGAATTGGTACCCA+TGG 0.555958 3_4:-67542842 Msa0516110:CDS
GGAATTCGCCATCGTGTGGA+AGG 0.557962 3_4:-67544070 Msa0516110:CDS
CACGTGCCCAAGTCCAACCA+TGG 0.566448 3_4:+67542825 None:intergenic
ACAAATACATGTGGATACCC+AGG 0.571175 3_4:-67544521 Msa0516110:CDS
CTGAATTGAATGATGATCTC+CGG 0.571320 3_4:-67544100 Msa0516110:CDS
GTGTTTGATTTGGGTTGAAG+AGG 0.577317 3_4:+67544642 None:intergenic
GGATTAGTGGTTCATTCGTG+TGG 0.578820 3_4:+67544573 None:intergenic
ATTGGGCACGAGGCATTCAG+TGG 0.599386 3_4:+67542566 None:intergenic
TATTATTACCTTGTCGTGGA+GGG 0.602530 3_4:+67543979 None:intergenic
CCCAAATCAAACACAAGCAG+TGG 0.605855 3_4:-67544633 Msa0516110:CDS
TCATGGAATTCGCCATCGTG+TGG 0.608164 3_4:-67544074 Msa0516110:CDS
ATGATGATCTCCGGTTGTCA+TGG 0.609684 3_4:-67544091 Msa0516110:CDS
TACAATGTCCCTCCACGACA+AGG 0.613340 3_4:-67543987 Msa0516110:intron
ACGCCATAGCCATATGTGCA+GGG 0.615668 3_4:-67542913 Msa0516110:CDS
GAGTCTCATGGTGGACGTTG+TGG 0.618506 3_4:-67544044 Msa0516110:CDS
TGTGGAAGGTGTGAGTCTCA+TGG 0.620175 3_4:-67544056 Msa0516110:CDS
TGCACAAACACCGTAAGTGA+AGG 0.621284 3_4:+67544200 None:intergenic
CTATTATTACCTTGTCGTGG+AGG 0.622703 3_4:+67543978 None:intergenic
CTTATCAAGACCTGAAGTGT+TGG 0.623889 3_4:+67542760 None:intergenic
GGAAAACATACAAATACATG+TGG 0.627739 3_4:-67544530 Msa0516110:CDS
CGCCATAGCCATATGTGCAG+GGG 0.628415 3_4:-67542912 Msa0516110:CDS
CAAATACATGTGGATACCCA+GGG 0.631304 3_4:-67544520 Msa0516110:CDS
AGCTATTGGTTTGTACCTAG+AGG 0.637453 3_4:+67544262 None:intergenic
AGAATATATGCCTAAAGAGA+CGG 0.642075 3_4:-67542648 Msa0516110:CDS
GACTCACACCTTCCACACGA+TGG 0.651360 3_4:+67544062 None:intergenic
ACGTGCCCAAGTCCAACCAT+GGG 0.657557 3_4:+67542826 None:intergenic
ACATTGACAGGGATTTCCCG+TGG 0.660325 3_4:-67542944 Msa0516110:intron
CTATGGCGTAACTTGCACCA+CGG 0.660502 3_4:+67542927 None:intergenic
ATACACTTACCAAAACCCAA+TGG 0.677744 3_4:-67542695 Msa0516110:CDS
TTACAATCACAAGCGAGAGG+TGG 0.686605 3_4:-67542521 Msa0516110:CDS
AGTTCTACAAATTGGGCACG+AGG 0.692854 3_4:+67542556 None:intergenic
AGGTTACAATCACAAGCGAG+AGG 0.700504 3_4:-67542524 Msa0516110:CDS
GGAAGGTGTGAGTCTCATGG+TGG 0.729696 3_4:-67544053 Msa0516110:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGATGTAAAAATTTAATAAA+AGG + chr3_4:67544019-67544038 None:intergenic 10.0%
!! TTAAAAATGTTTATTTACTA+CGG + chr3_4:67544075-67544094 None:intergenic 10.0%
!! AGATGTAAAAATTTAATAAA+AGG + chr3_4:67544019-67544038 None:intergenic 10.0%
!! TTAAAAATGTTTATTTACTA+CGG + chr3_4:67544075-67544094 None:intergenic 10.0%
!! ATATAATTAAAATACTCTTG+TGG - chr3_4:67543785-67543804 Msa0516110:intron 15.0%
!! TATAATTAAAATACTCTTGT+GGG - chr3_4:67543786-67543805 Msa0516110:intron 15.0%
!!! GATGAATTTTTTAATCATTT+TGG - chr3_4:67543820-67543839 Msa0516110:intron 15.0%
!! CAAATGCTAAAAATTTAAAA+AGG + chr3_4:67543907-67543926 None:intergenic 15.0%
!! ATATAATTAAAATACTCTTG+TGG - chr3_4:67543785-67543804 Msa0516110:intron 15.0%
!! TATAATTAAAATACTCTTGT+GGG - chr3_4:67543786-67543805 Msa0516110:intron 15.0%
!!! GATGAATTTTTTAATCATTT+TGG - chr3_4:67543820-67543839 Msa0516110:intron 15.0%
!! CAAATGCTAAAAATTTAAAA+AGG + chr3_4:67543907-67543926 None:intergenic 15.0%
!! TCTTGCGTATAAAAAAATAT+TGG + chr3_4:67543311-67543330 None:intergenic 20.0%
!!! AATATTTTTTTATACGCAAG+AGG - chr3_4:67543310-67543329 Msa0516110:intron 20.0%
!! GTAATATGATTAAAATGGTT+AGG + chr3_4:67543509-67543528 None:intergenic 20.0%
!! AAGGAGTAATATGATTAAAA+TGG + chr3_4:67543514-67543533 None:intergenic 20.0%
!! TTAAATTGTTCTTGAAGAAT+TGG + chr3_4:67543870-67543889 None:intergenic 20.0%
!! TCTTGCGTATAAAAAAATAT+TGG + chr3_4:67543311-67543330 None:intergenic 20.0%
!!! AATATTTTTTTATACGCAAG+AGG - chr3_4:67543310-67543329 Msa0516110:intron 20.0%
!! GTAATATGATTAAAATGGTT+AGG + chr3_4:67543509-67543528 None:intergenic 20.0%
!! AAGGAGTAATATGATTAAAA+TGG + chr3_4:67543514-67543533 None:intergenic 20.0%
!! TTAAATTGTTCTTGAAGAAT+TGG + chr3_4:67543870-67543889 None:intergenic 20.0%
! AAACAAATTAGGATGAGTTT+GGG + chr3_4:67542529-67542548 None:intergenic 25.0%
!! TTGTATGTTTTCCTTCTATA+TGG + chr3_4:67542660-67542679 None:intergenic 25.0%
!! ATACTAAGATTTTCCATGTA+AGG + chr3_4:67542754-67542773 None:intergenic 25.0%
!!! GTTTGGCATAGTTTATTTTT+TGG + chr3_4:67542777-67542796 None:intergenic 25.0%
!!! GGTAAATTGTTGATAGTAAA+CGG + chr3_4:67542881-67542900 None:intergenic 25.0%
!! CAAAAATGAATTGGTTCAAA+TGG + chr3_4:67543355-67543374 None:intergenic 25.0%
!! CATTTGAACCAATTCATTTT+TGG - chr3_4:67543353-67543372 Msa0516110:intron 25.0%
! AATGTAAATGATAAACGAGA+GGG + chr3_4:67543400-67543419 None:intergenic 25.0%
! AAATGTAAATGATAAACGAG+AGG + chr3_4:67543401-67543420 None:intergenic 25.0%
! GGAGAGAAAAAATATACTAA+GGG + chr3_4:67543448-67543467 None:intergenic 25.0%
! AGGAGAGAAAAAATATACTA+AGG + chr3_4:67543449-67543468 None:intergenic 25.0%
! ATAGCTGATTGATTAAACTA+AGG + chr3_4:67543533-67543552 None:intergenic 25.0%
! TTAATCAATCAGCTATCTTA+AGG - chr3_4:67543537-67543556 Msa0516110:intron 25.0%
!! TCCTTATTGTTTAAGTTTGA+AGG - chr3_4:67543568-67543587 Msa0516110:intron 25.0%
!! CCTTATTGTTTAAGTTTGAA+GGG - chr3_4:67543569-67543588 Msa0516110:intron 25.0%
! TTCCATATCTAATTAGTCAT+GGG + chr3_4:67543659-67543678 None:intergenic 25.0%
! TTTCCATATCTAATTAGTCA+TGG + chr3_4:67543660-67543679 None:intergenic 25.0%
! TCTTTCTCAACAAAAATTGT+CGG - chr3_4:67543927-67543946 Msa0516110:intron 25.0%
!! ATGAAATTTGAAACATTGAC+AGG - chr3_4:67544241-67544260 Msa0516110:CDS 25.0%
!! TGAAATTTGAAACATTGACA+GGG - chr3_4:67544242-67544261 Msa0516110:CDS 25.0%
! ATATATTCTGACAAGCATAT+TGG + chr3_4:67544538-67544557 None:intergenic 25.0%
! AAACAAATTAGGATGAGTTT+GGG + chr3_4:67542529-67542548 None:intergenic 25.0%
!! TTGTATGTTTTCCTTCTATA+TGG + chr3_4:67542660-67542679 None:intergenic 25.0%
!! ATACTAAGATTTTCCATGTA+AGG + chr3_4:67542754-67542773 None:intergenic 25.0%
!!! GTTTGGCATAGTTTATTTTT+TGG + chr3_4:67542777-67542796 None:intergenic 25.0%
!!! GGTAAATTGTTGATAGTAAA+CGG + chr3_4:67542881-67542900 None:intergenic 25.0%
!! CAAAAATGAATTGGTTCAAA+TGG + chr3_4:67543355-67543374 None:intergenic 25.0%
!! CATTTGAACCAATTCATTTT+TGG - chr3_4:67543353-67543372 Msa0516110:intron 25.0%
! AATGTAAATGATAAACGAGA+GGG + chr3_4:67543400-67543419 None:intergenic 25.0%
! AAATGTAAATGATAAACGAG+AGG + chr3_4:67543401-67543420 None:intergenic 25.0%
! GGAGAGAAAAAATATACTAA+GGG + chr3_4:67543448-67543467 None:intergenic 25.0%
! AGGAGAGAAAAAATATACTA+AGG + chr3_4:67543449-67543468 None:intergenic 25.0%
! ATAGCTGATTGATTAAACTA+AGG + chr3_4:67543533-67543552 None:intergenic 25.0%
! TTAATCAATCAGCTATCTTA+AGG - chr3_4:67543537-67543556 Msa0516110:intron 25.0%
!! TCCTTATTGTTTAAGTTTGA+AGG - chr3_4:67543568-67543587 Msa0516110:intron 25.0%
!! CCTTATTGTTTAAGTTTGAA+GGG - chr3_4:67543569-67543588 Msa0516110:intron 25.0%
! TTCCATATCTAATTAGTCAT+GGG + chr3_4:67543659-67543678 None:intergenic 25.0%
! TTTCCATATCTAATTAGTCA+TGG + chr3_4:67543660-67543679 None:intergenic 25.0%
! TCTTTCTCAACAAAAATTGT+CGG - chr3_4:67543927-67543946 Msa0516110:intron 25.0%
!! ATGAAATTTGAAACATTGAC+AGG - chr3_4:67544241-67544260 Msa0516110:CDS 25.0%
!! TGAAATTTGAAACATTGACA+GGG - chr3_4:67544242-67544261 Msa0516110:CDS 25.0%
! ATATATTCTGACAAGCATAT+TGG + chr3_4:67544538-67544557 None:intergenic 25.0%
GAAACAAATTAGGATGAGTT+TGG + chr3_4:67542530-67542549 None:intergenic 30.0%
AGAGGAAAAGGAAACAAATT+AGG + chr3_4:67542540-67542559 None:intergenic 30.0%
! GTTTTCCTTCTATATGGAAT+GGG + chr3_4:67542654-67542673 None:intergenic 30.0%
! TGTTTTCCTTCTATATGGAA+TGG + chr3_4:67542655-67542674 None:intergenic 30.0%
GGAAAACATACAAATACATG+TGG - chr3_4:67542667-67542686 Msa0516110:CDS 30.0%
TTTCCATGTAAGGTAGATTA+AGG + chr3_4:67542744-67542763 None:intergenic 30.0%
GTTCACAAAAAGTTCTTGTT+TGG + chr3_4:67542794-67542813 None:intergenic 30.0%
GTTGTATGAGCAGTTAAAAA+AGG + chr3_4:67542902-67542921 None:intergenic 30.0%
!! TTTTAACTGCTCATACAACT+TGG - chr3_4:67542903-67542922 Msa0516110:CDS 30.0%
TAATCACTAAGACAAGCTAT+TGG + chr3_4:67542952-67542971 None:intergenic 30.0%
TGTTGCAAAAATGTTGTACT+TGG + chr3_4:67542977-67542996 None:intergenic 30.0%
GACAACACTTGATCAAAAAA+CGG + chr3_4:67543075-67543094 None:intergenic 30.0%
!! TTGAACCAATTCATTTTTGG+TGG - chr3_4:67543356-67543375 Msa0516110:intron 30.0%
TTGATCCACCAAAAATGAAT+TGG + chr3_4:67543364-67543383 None:intergenic 30.0%
! AAAATATACTAAGGGTGCAT+TGG + chr3_4:67543440-67543459 None:intergenic 30.0%
CCCTTCAAACTTAAACAATA+AGG + chr3_4:67543572-67543591 None:intergenic 30.0%
TTCCCATGACTAATTAGATA+TGG - chr3_4:67543654-67543673 Msa0516110:intron 30.0%
! AATTTGAAGTTTCAAACAGC+AGG - chr3_4:67543678-67543697 Msa0516110:intron 30.0%
! ATTTGAAGTTTCAAACAGCA+GGG - chr3_4:67543679-67543698 Msa0516110:intron 30.0%
ATATGTAGCAAGTTCAGAAT+TGG - chr3_4:67544346-67544365 Msa0516110:CDS 30.0%
TTTGGGTATGATTCAATTGT+TGG + chr3_4:67544463-67544482 None:intergenic 30.0%
TCATACCCAAAAATAGTGAT+AGG - chr3_4:67544472-67544491 Msa0516110:CDS 30.0%
!! CATCACCTATCACTATTTTT+GGG + chr3_4:67544480-67544499 None:intergenic 30.0%
! TCATCACCTATCACTATTTT+TGG + chr3_4:67544481-67544500 None:intergenic 30.0%
AGAATATATGCCTAAAGAGA+CGG - chr3_4:67544549-67544568 Msa0516110:CDS 30.0%
GAAACAAATTAGGATGAGTT+TGG + chr3_4:67542530-67542549 None:intergenic 30.0%
AGAGGAAAAGGAAACAAATT+AGG + chr3_4:67542540-67542559 None:intergenic 30.0%
! GTTTTCCTTCTATATGGAAT+GGG + chr3_4:67542654-67542673 None:intergenic 30.0%
! TGTTTTCCTTCTATATGGAA+TGG + chr3_4:67542655-67542674 None:intergenic 30.0%
GGAAAACATACAAATACATG+TGG - chr3_4:67542667-67542686 Msa0516110:CDS 30.0%
TTTCCATGTAAGGTAGATTA+AGG + chr3_4:67542744-67542763 None:intergenic 30.0%
GTTCACAAAAAGTTCTTGTT+TGG + chr3_4:67542794-67542813 None:intergenic 30.0%
GTTGTATGAGCAGTTAAAAA+AGG + chr3_4:67542902-67542921 None:intergenic 30.0%
!! TTTTAACTGCTCATACAACT+TGG - chr3_4:67542903-67542922 Msa0516110:CDS 30.0%
TAATCACTAAGACAAGCTAT+TGG + chr3_4:67542952-67542971 None:intergenic 30.0%
TGTTGCAAAAATGTTGTACT+TGG + chr3_4:67542977-67542996 None:intergenic 30.0%
GACAACACTTGATCAAAAAA+CGG + chr3_4:67543075-67543094 None:intergenic 30.0%
!! TTGAACCAATTCATTTTTGG+TGG - chr3_4:67543356-67543375 Msa0516110:intron 30.0%
TTGATCCACCAAAAATGAAT+TGG + chr3_4:67543364-67543383 None:intergenic 30.0%
! AAAATATACTAAGGGTGCAT+TGG + chr3_4:67543440-67543459 None:intergenic 30.0%
CCCTTCAAACTTAAACAATA+AGG + chr3_4:67543572-67543591 None:intergenic 30.0%
TTCCCATGACTAATTAGATA+TGG - chr3_4:67543654-67543673 Msa0516110:intron 30.0%
! AATTTGAAGTTTCAAACAGC+AGG - chr3_4:67543678-67543697 Msa0516110:intron 30.0%
! ATTTGAAGTTTCAAACAGCA+GGG - chr3_4:67543679-67543698 Msa0516110:intron 30.0%
ATATGTAGCAAGTTCAGAAT+TGG - chr3_4:67544346-67544365 Msa0516110:CDS 30.0%
TTTGGGTATGATTCAATTGT+TGG + chr3_4:67544463-67544482 None:intergenic 30.0%
TCATACCCAAAAATAGTGAT+AGG - chr3_4:67544472-67544491 Msa0516110:CDS 30.0%
!! CATCACCTATCACTATTTTT+GGG + chr3_4:67544480-67544499 None:intergenic 30.0%
! TCATCACCTATCACTATTTT+TGG + chr3_4:67544481-67544500 None:intergenic 30.0%
AGAATATATGCCTAAAGAGA+CGG - chr3_4:67544549-67544568 Msa0516110:CDS 30.0%
! ATTAGGATGAGTTTGGGATT+GGG + chr3_4:67542523-67542542 None:intergenic 35.0%
! AATTAGGATGAGTTTGGGAT+TGG + chr3_4:67542524-67542543 None:intergenic 35.0%
! AGTGTCTAAGCATGTTTCTA+TGG + chr3_4:67542599-67542618 None:intergenic 35.0%
CTTCTATATGGAATGGGAAT+CGG + chr3_4:67542648-67542667 None:intergenic 35.0%
TGCATAATACTTTGAACCCT+GGG + chr3_4:67542696-67542715 None:intergenic 35.0%
TTGCATAATACTTTGAACCC+TGG + chr3_4:67542697-67542716 None:intergenic 35.0%
!! AAGGTAGATTAAGGAGTGTT+TGG + chr3_4:67542735-67542754 None:intergenic 35.0%
ACTCCTTAATCTACCTTACA+TGG - chr3_4:67542738-67542757 Msa0516110:CDS 35.0%
!!! TGTTTTGGGAGACAGTTATT+GGG + chr3_4:67542820-67542839 None:intergenic 35.0%
!!! TTGTTTTGGGAGACAGTTAT+TGG + chr3_4:67542821-67542840 None:intergenic 35.0%
!! TGAGTGAGAGAAGTTGTTTT+GGG + chr3_4:67542834-67542853 None:intergenic 35.0%
! TTGAGTGAGAGAAGTTGTTT+TGG + chr3_4:67542835-67542854 None:intergenic 35.0%
TGATAGTAAACGGCATCAAA+AGG + chr3_4:67542871-67542890 None:intergenic 35.0%
ACTTGGAATATCTTACCTCT+AGG - chr3_4:67542920-67542939 Msa0516110:CDS 35.0%
! TTTTGCAACACCTTCACTTA+CGG - chr3_4:67542987-67543006 Msa0516110:intron 35.0%
TCAAAAAACGGTGATTGAAC+CGG + chr3_4:67543063-67543082 None:intergenic 35.0%
CTGAATTGAATGATGATCTC+CGG - chr3_4:67543097-67543116 Msa0516110:intron 35.0%
TATTATTACCTTGTCGTGGA+GGG + chr3_4:67543221-67543240 None:intergenic 35.0%
GTACTATTATTACCTTGTCG+TGG + chr3_4:67543225-67543244 None:intergenic 35.0%
!! GGTGCATTGGTTGAATAAAA+AGG + chr3_4:67543427-67543446 None:intergenic 35.0%
GAAATCTCGAACATAATCGA+AGG + chr3_4:67543474-67543493 None:intergenic 35.0%
TGAAGGGAGATTATTAGAGA+CGG - chr3_4:67543585-67543604 Msa0516110:intron 35.0%
! TTGTCAATTTTTCCTCTCCA+TGG - chr3_4:67544142-67544161 Msa0516110:CDS 35.0%
! TGTCAATTTTTCCTCTCCAT+GGG - chr3_4:67544143-67544162 Msa0516110:CDS 35.0%
GCTTCATTTCACTATTAAGC+TGG - chr3_4:67544323-67544342 Msa0516110:CDS 35.0%
ATACACTTACCAAAACCCAA+TGG - chr3_4:67544502-67544521 Msa0516110:CDS 35.0%
ATTGGACATGTCTTACCATT+GGG + chr3_4:67544520-67544539 None:intergenic 35.0%
TATTGGACATGTCTTACCAT+TGG + chr3_4:67544521-67544540 None:intergenic 35.0%
! ATAGTTTTCACCGTCTCTTT+AGG + chr3_4:67544562-67544581 None:intergenic 35.0%
TGAAAGCAATGTTCACATTC+AGG + chr3_4:67544586-67544605 None:intergenic 35.0%
! ATGCACAATGCATTGATGAA+TGG - chr3_4:67544605-67544624 Msa0516110:CDS 35.0%
GAGGAGAAGTTCTACAAATT+GGG + chr3_4:67544651-67544670 None:intergenic 35.0%
! ATTAGGATGAGTTTGGGATT+GGG + chr3_4:67542523-67542542 None:intergenic 35.0%
! AATTAGGATGAGTTTGGGAT+TGG + chr3_4:67542524-67542543 None:intergenic 35.0%
! AGTGTCTAAGCATGTTTCTA+TGG + chr3_4:67542599-67542618 None:intergenic 35.0%
CTTCTATATGGAATGGGAAT+CGG + chr3_4:67542648-67542667 None:intergenic 35.0%
TGCATAATACTTTGAACCCT+GGG + chr3_4:67542696-67542715 None:intergenic 35.0%
TTGCATAATACTTTGAACCC+TGG + chr3_4:67542697-67542716 None:intergenic 35.0%
!! AAGGTAGATTAAGGAGTGTT+TGG + chr3_4:67542735-67542754 None:intergenic 35.0%
ACTCCTTAATCTACCTTACA+TGG - chr3_4:67542738-67542757 Msa0516110:CDS 35.0%
!!! TGTTTTGGGAGACAGTTATT+GGG + chr3_4:67542820-67542839 None:intergenic 35.0%
!!! TTGTTTTGGGAGACAGTTAT+TGG + chr3_4:67542821-67542840 None:intergenic 35.0%
!! TGAGTGAGAGAAGTTGTTTT+GGG + chr3_4:67542834-67542853 None:intergenic 35.0%
! TTGAGTGAGAGAAGTTGTTT+TGG + chr3_4:67542835-67542854 None:intergenic 35.0%
TGATAGTAAACGGCATCAAA+AGG + chr3_4:67542871-67542890 None:intergenic 35.0%
ACTTGGAATATCTTACCTCT+AGG - chr3_4:67542920-67542939 Msa0516110:CDS 35.0%
! TTTTGCAACACCTTCACTTA+CGG - chr3_4:67542987-67543006 Msa0516110:intron 35.0%
TCAAAAAACGGTGATTGAAC+CGG + chr3_4:67543063-67543082 None:intergenic 35.0%
CTGAATTGAATGATGATCTC+CGG - chr3_4:67543097-67543116 Msa0516110:intron 35.0%
TATTATTACCTTGTCGTGGA+GGG + chr3_4:67543221-67543240 None:intergenic 35.0%
GTACTATTATTACCTTGTCG+TGG + chr3_4:67543225-67543244 None:intergenic 35.0%
!! GGTGCATTGGTTGAATAAAA+AGG + chr3_4:67543427-67543446 None:intergenic 35.0%
GAAATCTCGAACATAATCGA+AGG + chr3_4:67543474-67543493 None:intergenic 35.0%
TGAAGGGAGATTATTAGAGA+CGG - chr3_4:67543585-67543604 Msa0516110:intron 35.0%
! TTGTCAATTTTTCCTCTCCA+TGG - chr3_4:67544142-67544161 Msa0516110:CDS 35.0%
! TGTCAATTTTTCCTCTCCAT+GGG - chr3_4:67544143-67544162 Msa0516110:CDS 35.0%
GCTTCATTTCACTATTAAGC+TGG - chr3_4:67544323-67544342 Msa0516110:CDS 35.0%
ATACACTTACCAAAACCCAA+TGG - chr3_4:67544502-67544521 Msa0516110:CDS 35.0%
ATTGGACATGTCTTACCATT+GGG + chr3_4:67544520-67544539 None:intergenic 35.0%
TATTGGACATGTCTTACCAT+TGG + chr3_4:67544521-67544540 None:intergenic 35.0%
! ATAGTTTTCACCGTCTCTTT+AGG + chr3_4:67544562-67544581 None:intergenic 35.0%
TGAAAGCAATGTTCACATTC+AGG + chr3_4:67544586-67544605 None:intergenic 35.0%
! ATGCACAATGCATTGATGAA+TGG - chr3_4:67544605-67544624 Msa0516110:CDS 35.0%
GAGGAGAAGTTCTACAAATT+GGG + chr3_4:67544651-67544670 None:intergenic 35.0%
!! GATTTGGGTTGAAGAGGAAA+AGG + chr3_4:67542552-67542571 None:intergenic 40.0%
!! GTGTTTGATTTGGGTTGAAG+AGG + chr3_4:67542558-67542577 None:intergenic 40.0%
! CCACTGCTTGTGTTTGATTT+GGG + chr3_4:67542567-67542586 None:intergenic 40.0%
CGATTCCCATTCCATATAGA+AGG - chr3_4:67542646-67542665 Msa0516110:CDS 40.0%
ACAAATACATGTGGATACCC+AGG - chr3_4:67542676-67542695 Msa0516110:CDS 40.0%
CAAATACATGTGGATACCCA+GGG - chr3_4:67542677-67542696 Msa0516110:CDS 40.0%
!! AGCTATTGGTTTGTACCTAG+AGG + chr3_4:67542938-67542957 None:intergenic 40.0%
CTTGTCGTCTGTTTGTGATT+TGG - chr3_4:67543020-67543039 Msa0516110:intron 40.0%
CTATTATTACCTTGTCGTGG+AGG + chr3_4:67543222-67543241 None:intergenic 40.0%
GATAATTCACCTTGTGTGCT+CGG + chr3_4:67543279-67543298 None:intergenic 40.0%
! AAGAATTGGTCTGCGATTCA+CGG + chr3_4:67543856-67543875 None:intergenic 40.0%
TAAATTCTCAACCCATGGAG+AGG + chr3_4:67544157-67544176 None:intergenic 40.0%
CCATCTAAATTCTCAACCCA+TGG + chr3_4:67544162-67544181 None:intergenic 40.0%
! CCATGGGTTGAGAATTTAGA+TGG - chr3_4:67544159-67544178 Msa0516110:CDS 40.0%
! TGGGTTGAGAATTTAGATGG+TGG - chr3_4:67544162-67544181 Msa0516110:CDS 40.0%
GAAATGAAGCACAACAAAGC+TGG + chr3_4:67544313-67544332 None:intergenic 40.0%
AAGTTCAGAATTGGTACCCA+TGG - chr3_4:67544355-67544374 Msa0516110:CDS 40.0%
CATGAATACACCAACACTTC+AGG - chr3_4:67544427-67544446 Msa0516110:CDS 40.0%
CTTATCAAGACCTGAAGTGT+TGG + chr3_4:67544440-67544459 None:intergenic 40.0%
! CATGTCTTACCATTGGGTTT+TGG + chr3_4:67544514-67544533 None:intergenic 40.0%
GGAGGAGAAGTTCTACAAAT+TGG + chr3_4:67544652-67544671 None:intergenic 40.0%
TTTGTAGAACTTCTCCTCCA+AGG - chr3_4:67544653-67544672 Msa0516110:CDS 40.0%
!! GATTTGGGTTGAAGAGGAAA+AGG + chr3_4:67542552-67542571 None:intergenic 40.0%
!! GTGTTTGATTTGGGTTGAAG+AGG + chr3_4:67542558-67542577 None:intergenic 40.0%
! CCACTGCTTGTGTTTGATTT+GGG + chr3_4:67542567-67542586 None:intergenic 40.0%
CGATTCCCATTCCATATAGA+AGG - chr3_4:67542646-67542665 Msa0516110:CDS 40.0%
ACAAATACATGTGGATACCC+AGG - chr3_4:67542676-67542695 Msa0516110:CDS 40.0%
CAAATACATGTGGATACCCA+GGG - chr3_4:67542677-67542696 Msa0516110:CDS 40.0%
!! AGCTATTGGTTTGTACCTAG+AGG + chr3_4:67542938-67542957 None:intergenic 40.0%
CTTGTCGTCTGTTTGTGATT+TGG - chr3_4:67543020-67543039 Msa0516110:intron 40.0%
CTATTATTACCTTGTCGTGG+AGG + chr3_4:67543222-67543241 None:intergenic 40.0%
GATAATTCACCTTGTGTGCT+CGG + chr3_4:67543279-67543298 None:intergenic 40.0%
! AAGAATTGGTCTGCGATTCA+CGG + chr3_4:67543856-67543875 None:intergenic 40.0%
TAAATTCTCAACCCATGGAG+AGG + chr3_4:67544157-67544176 None:intergenic 40.0%
CCATCTAAATTCTCAACCCA+TGG + chr3_4:67544162-67544181 None:intergenic 40.0%
! CCATGGGTTGAGAATTTAGA+TGG - chr3_4:67544159-67544178 Msa0516110:CDS 40.0%
! TGGGTTGAGAATTTAGATGG+TGG - chr3_4:67544162-67544181 Msa0516110:CDS 40.0%
GAAATGAAGCACAACAAAGC+TGG + chr3_4:67544313-67544332 None:intergenic 40.0%
AAGTTCAGAATTGGTACCCA+TGG - chr3_4:67544355-67544374 Msa0516110:CDS 40.0%
CATGAATACACCAACACTTC+AGG - chr3_4:67544427-67544446 Msa0516110:CDS 40.0%
CTTATCAAGACCTGAAGTGT+TGG + chr3_4:67544440-67544459 None:intergenic 40.0%
! CATGTCTTACCATTGGGTTT+TGG + chr3_4:67544514-67544533 None:intergenic 40.0%
GGAGGAGAAGTTCTACAAAT+TGG + chr3_4:67544652-67544671 None:intergenic 40.0%
TTTGTAGAACTTCTCCTCCA+AGG - chr3_4:67544653-67544672 Msa0516110:CDS 40.0%
CCCAAATCAAACACAAGCAG+TGG - chr3_4:67542564-67542583 Msa0516110:CDS 45.0%
GCCACTGCTTGTGTTTGATT+TGG + chr3_4:67542568-67542587 None:intergenic 45.0%
! GGATTAGTGGTTCATTCGTG+TGG + chr3_4:67542627-67542646 None:intergenic 45.0%
! TGGAATGGGAATCGGATTAG+TGG + chr3_4:67542640-67542659 None:intergenic 45.0%
CATCAAAAGGAGAGCTAGAG+AGG + chr3_4:67542858-67542877 None:intergenic 45.0%
TGCACAAACACCGTAAGTGA+AGG + chr3_4:67543000-67543019 None:intergenic 45.0%
TGATTGAACCGGCACGTTAA+CGG + chr3_4:67543052-67543071 None:intergenic 45.0%
ATGATGATCTCCGGTTGTCA+TGG - chr3_4:67543106-67543125 Msa0516110:intron 45.0%
ATGGCGAATTCCATGACAAC+CGG + chr3_4:67543119-67543138 None:intergenic 45.0%
ATGAATATGCCGAGCACACA+AGG - chr3_4:67543267-67543286 Msa0516110:intron 45.0%
CTCGAACATAATCGAAGGCA+AGG + chr3_4:67543469-67543488 None:intergenic 45.0%
!! TCTGCGATTCACGGTTTTAG+TGG + chr3_4:67543847-67543866 None:intergenic 45.0%
TCAGAATTGGTACCCATGGT+TGG - chr3_4:67544359-67544378 Msa0516110:CDS 45.0%
AGTTCTACAAATTGGGCACG+AGG + chr3_4:67544644-67544663 None:intergenic 45.0%
GCTTGTGATTGTAACCTTGG+AGG + chr3_4:67544670-67544689 None:intergenic 45.0%
CTCGCTTGTGATTGTAACCT+TGG + chr3_4:67544673-67544692 None:intergenic 45.0%
AGGTTACAATCACAAGCGAG+AGG - chr3_4:67544673-67544692 Msa0516110:CDS 45.0%
TTACAATCACAAGCGAGAGG+TGG - chr3_4:67544676-67544695 Msa0516110:CDS 45.0%
CCCAAATCAAACACAAGCAG+TGG - chr3_4:67542564-67542583 Msa0516110:CDS 45.0%
GCCACTGCTTGTGTTTGATT+TGG + chr3_4:67542568-67542587 None:intergenic 45.0%
! GGATTAGTGGTTCATTCGTG+TGG + chr3_4:67542627-67542646 None:intergenic 45.0%
! TGGAATGGGAATCGGATTAG+TGG + chr3_4:67542640-67542659 None:intergenic 45.0%
CATCAAAAGGAGAGCTAGAG+AGG + chr3_4:67542858-67542877 None:intergenic 45.0%
TGCACAAACACCGTAAGTGA+AGG + chr3_4:67543000-67543019 None:intergenic 45.0%
TGATTGAACCGGCACGTTAA+CGG + chr3_4:67543052-67543071 None:intergenic 45.0%
ATGATGATCTCCGGTTGTCA+TGG - chr3_4:67543106-67543125 Msa0516110:intron 45.0%
ATGGCGAATTCCATGACAAC+CGG + chr3_4:67543119-67543138 None:intergenic 45.0%
ATGAATATGCCGAGCACACA+AGG - chr3_4:67543267-67543286 Msa0516110:intron 45.0%
CTCGAACATAATCGAAGGCA+AGG + chr3_4:67543469-67543488 None:intergenic 45.0%
!! TCTGCGATTCACGGTTTTAG+TGG + chr3_4:67543847-67543866 None:intergenic 45.0%
TCAGAATTGGTACCCATGGT+TGG - chr3_4:67544359-67544378 Msa0516110:CDS 45.0%
AGTTCTACAAATTGGGCACG+AGG + chr3_4:67544644-67544663 None:intergenic 45.0%
GCTTGTGATTGTAACCTTGG+AGG + chr3_4:67544670-67544689 None:intergenic 45.0%
CTCGCTTGTGATTGTAACCT+TGG + chr3_4:67544673-67544692 None:intergenic 45.0%
AGGTTACAATCACAAGCGAG+AGG - chr3_4:67544673-67544692 Msa0516110:CDS 45.0%
TTACAATCACAAGCGAGAGG+TGG - chr3_4:67544676-67544695 Msa0516110:CDS 45.0%
! GGTTGATACCGTTAACGTGC+CGG - chr3_4:67543041-67543060 Msa0516110:intron 50.0%
TCATGGAATTCGCCATCGTG+TGG - chr3_4:67543123-67543142 Msa0516110:intron 50.0%
! TGTGGAAGGTGTGAGTCTCA+TGG - chr3_4:67543141-67543160 Msa0516110:intron 50.0%
TACAATGTCCCTCCACGACA+AGG - chr3_4:67543210-67543229 Msa0516110:intron 50.0%
ACATTGACAGGGATTTCCCG+TGG - chr3_4:67544253-67544272 Msa0516110:CDS 50.0%
! TATGGCGTAACTTGCACCAC+GGG + chr3_4:67544272-67544291 None:intergenic 50.0%
! CTATGGCGTAACTTGCACCA+CGG + chr3_4:67544273-67544292 None:intergenic 50.0%
TACGCCATAGCCATATGTGC+AGG - chr3_4:67544283-67544302 Msa0516110:CDS 50.0%
ACGCCATAGCCATATGTGCA+GGG - chr3_4:67544284-67544303 Msa0516110:CDS 50.0%
TTGGTACCCATGGTTGGACT+TGG - chr3_4:67544365-67544384 Msa0516110:CDS 50.0%
TGGTACCCATGGTTGGACTT+GGG - chr3_4:67544366-67544385 Msa0516110:CDS 50.0%
! GGTTGATACCGTTAACGTGC+CGG - chr3_4:67543041-67543060 Msa0516110:intron 50.0%
TCATGGAATTCGCCATCGTG+TGG - chr3_4:67543123-67543142 Msa0516110:intron 50.0%
! TGTGGAAGGTGTGAGTCTCA+TGG - chr3_4:67543141-67543160 Msa0516110:intron 50.0%
TACAATGTCCCTCCACGACA+AGG - chr3_4:67543210-67543229 Msa0516110:intron 50.0%
ACATTGACAGGGATTTCCCG+TGG - chr3_4:67544253-67544272 Msa0516110:CDS 50.0%
! TATGGCGTAACTTGCACCAC+GGG + chr3_4:67544272-67544291 None:intergenic 50.0%
! CTATGGCGTAACTTGCACCA+CGG + chr3_4:67544273-67544292 None:intergenic 50.0%
TACGCCATAGCCATATGTGC+AGG - chr3_4:67544283-67544302 Msa0516110:CDS 50.0%
ACGCCATAGCCATATGTGCA+GGG - chr3_4:67544284-67544303 Msa0516110:CDS 50.0%
TTGGTACCCATGGTTGGACT+TGG - chr3_4:67544365-67544384 Msa0516110:CDS 50.0%
TGGTACCCATGGTTGGACTT+GGG - chr3_4:67544366-67544385 Msa0516110:CDS 50.0%
GGAATTCGCCATCGTGTGGA+AGG - chr3_4:67543127-67543146 Msa0516110:intron 55.0%
GACTCACACCTTCCACACGA+TGG + chr3_4:67543138-67543157 None:intergenic 55.0%
! GGAAGGTGTGAGTCTCATGG+TGG - chr3_4:67543144-67543163 Msa0516110:intron 55.0%
!! GAGTCTCATGGTGGACGTTG+TGG - chr3_4:67543153-67543172 Msa0516110:intron 55.0%
CGCCATAGCCATATGTGCAG+GGG - chr3_4:67544285-67544304 Msa0516110:CDS 55.0%
CACCCCTGCACATATGGCTA+TGG + chr3_4:67544290-67544309 None:intergenic 55.0%
ACGTGCCCAAGTCCAACCAT+GGG + chr3_4:67544374-67544393 None:intergenic 55.0%
! ATTGGGCACGAGGCATTCAG+TGG + chr3_4:67544634-67544653 None:intergenic 55.0%
GGAATTCGCCATCGTGTGGA+AGG - chr3_4:67543127-67543146 Msa0516110:intron 55.0%
GACTCACACCTTCCACACGA+TGG + chr3_4:67543138-67543157 None:intergenic 55.0%
! GGAAGGTGTGAGTCTCATGG+TGG - chr3_4:67543144-67543163 Msa0516110:intron 55.0%
!! GAGTCTCATGGTGGACGTTG+TGG - chr3_4:67543153-67543172 Msa0516110:intron 55.0%
CGCCATAGCCATATGTGCAG+GGG - chr3_4:67544285-67544304 Msa0516110:CDS 55.0%
CACCCCTGCACATATGGCTA+TGG + chr3_4:67544290-67544309 None:intergenic 55.0%
ACGTGCCCAAGTCCAACCAT+GGG + chr3_4:67544374-67544393 None:intergenic 55.0%
! ATTGGGCACGAGGCATTCAG+TGG + chr3_4:67544634-67544653 None:intergenic 55.0%
AGCTGGCACCCCTGCACATA+TGG + chr3_4:67544296-67544315 None:intergenic 60.0%
CACGTGCCCAAGTCCAACCA+TGG + chr3_4:67544375-67544394 None:intergenic 60.0%
AGCTGGCACCCCTGCACATA+TGG + chr3_4:67544296-67544315 None:intergenic 60.0%
CACGTGCCCAAGTCCAACCA+TGG + chr3_4:67544375-67544394 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 67542511 67544708 67542511 ID=Msa0516110;Name=Msa0516110
chr3_4 mRNA 67542511 67544708 67542511 ID=Msa0516110-mRNA-1;Parent=Msa0516110;Name=Msa0516110-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|1|0|1|1|1|2|0|388
chr3_4 exon 67543988 67544708 67543988 ID=Msa0516110-mRNA-1:exon:17612;Parent=Msa0516110-mRNA-1
chr3_4 exon 67542511 67542956 67542511 ID=Msa0516110-mRNA-1:exon:17611;Parent=Msa0516110-mRNA-1
chr3_4 CDS 67543988 67544708 67543988 ID=Msa0516110-mRNA-1:cds;Parent=Msa0516110-mRNA-1
chr3_4 CDS 67542511 67542956 67542511 ID=Msa0516110-mRNA-1:cds;Parent=Msa0516110-mRNA-1
Gene Sequence

>Msa0516110

ATGAAGACTCCCAATCCCAAACTCATCCTAATTTGTTTCCTTTTCCTCTTCAACCCAAATCAAACACAAGCAGTGGCACTAGCAACCATAGAAACATGCTTAGACACTGTTTGCCACACGAATGAACCACTAATCCGATTCCCATTCCATATAGAAGGAAAACATACAAATACATGTGGATACCCAGGGTTCAAAGTATTATGCAATGAAAGTGAAAAAAGCCAAACACTCCTTAATCTACCTTACATGGAAAATCTTAGTATCCAAAAAATAAACTATGCCAAACAAGAACTTTTTGTGAACGATCCCAATAACTGTCTCCCAAAACAACTTCTCTCACTCAACCTCTCTAGCTCTCCTTTTGATGCCGTTTACTATCAACAATTTACCTTTTTTAACTGCTCATACAACTTGGAATATCTTACCTCTAGGTACAAACCAATAGCTTGTCTTAGTGATTATTCCAAGTACAACATTTTTGCAACACCTTCACTTACGGTGTTTGTGCACTTGTCGTCTGTTTGTGATTTGGTTGATACCGTTAACGTGCCGGTTCAATCACCGTTTTTTGATCAAGTGTTGTCGTCTGAATTGAATGATGATCTCCGGTTGTCATGGAATTCGCCATCGTGTGGAAGGTGTGAGTCTCATGGTGGACGTTGTGGCTTCAAAAATAACTCTACTTTTGAAATTGCTTGCTACAATGTCCCTCCACGACAAGGGATTTCCCGTGGTGCAAGTTACGCCATAGCCATATGTGCAGGGGTGCCAGCTTTGTTGTGCTTCATTTCACTATTAAGCTGGATATGTAGCAAGTTCAGAATTGGTACCCATGGTTGGACTTGGGCACGTGAGACAATTGCAGATTTTGAGTCTCTTCTTGATCATGAATACACCAACACTTCAGGTCTTGATAAGCCAACAATTGAATCATACCCAAAAATAGTGATAGGTGATGACATACACTTACCAAAACCCAATGGTAAGACATGTCCAATATGCTTGTCAGAATATATGCCTAAAGAGACGGTGAAAACTATGCCTGAATGTGAACATTGCTTTCATGCACAATGCATTGATGAATGGCTTCCACTGAATGCCTCGTGCCCAATTTGTAGAACTTCTCCTCCAAGGTTACAATCACAAGCGAGAGGTGGTAGTGACTAG
Protein sequence

>Msa0516110

MKTPNPKLILICFLFLFNPNQTQAVALATIETCLDTVCHTNEPLIRFPFHIEGKHTNTCGYPGFKVLCNESEKSQTLLNLPYMENLSIQKINYAKQELFVNDPNNCLPKQLLSLNLSSSPFDAVYYQQFTFFNCSYNLEYLTSRYKPIACLSDYSKYNIFATPSLTVFVHLSSVCDLVDTVNVPVQSPFFDQVLSSELNDDLRLSWNSPSCGRCESHGGRCGFKNNSTFEIACYNVPPRQGISRGASYAIAICAGVPALLCFISLLSWICSKFRIGTHGWTWARETIADFESLLDHEYTNTSGLDKPTIESYPKIVIGDDIHLPKPNGKTCPICLSEYMPKETVKTMPECEHCFHAQCIDEWLPLNASCPICRTSPPRLQSQARGGSD*