AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0132250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0132250 MtrunA17_Chr1g0201331 99.000 300 3 0 1 300 1 300 0.0 600
Msa0132250 MtrunA17_Chr4g0001221 59.375 288 102 2 9 287 7 288 1.27e-113 330
Msa0132250 MtrunA17_Chr2g0333771 56.908 304 111 4 13 300 11 310 2.52e-108 317
Msa0132250 MtrunA17_Chr4g0001111 54.839 217 83 2 9 216 7 217 1.70e-72 223
Msa0132250 MtrunA17_Chr8g0379911 49.356 233 107 3 71 300 35 259 2.68e-69 216
Msa0132250 MtrunA17_Chr8g0379901 49.362 235 108 3 69 300 34 260 3.25e-69 216
Msa0132250 MtrunA17_Chr2g0284581 47.600 250 112 4 54 300 26 259 2.36e-67 211
Msa0132250 MtrunA17_Chr2g0284631 51.852 216 103 1 69 283 86 301 3.72e-66 210
Msa0132250 MtrunA17_Chr2g0284591 48.718 234 109 3 70 300 32 257 5.24e-66 207
Msa0132250 MtrunA17_Chr2g0284611 48.696 230 113 2 74 300 38 265 8.55e-65 204
Msa0132250 MtrunA17_Chr7g0217331 44.749 219 121 0 72 290 35 253 1.73e-64 204
Msa0132250 MtrunA17_Chr2g0284601 49.057 212 97 3 92 300 8 211 2.08e-59 189
Msa0132250 MtrunA17_Chr5g0426131 39.706 272 137 5 28 294 6 255 6.26e-58 186
Msa0132250 MtrunA17_Chr4g0001161 63.571 140 51 0 87 226 1 140 3.91e-56 178
Msa0132250 MtrunA17_Chr1g0184541 43.111 225 128 0 69 293 57 281 1.29e-54 179
Msa0132250 MtrunA17_Chr5g0435051 39.921 253 138 3 32 283 10 249 5.16e-52 171
Msa0132250 MtrunA17_Chr4g0044201 42.373 236 110 5 70 300 31 245 1.22e-51 170
Msa0132250 MtrunA17_Chr3g0079421 41.004 239 135 4 47 283 1 235 3.71e-49 164
Msa0132250 MtrunA17_Chr4g0001151 60.305 131 37 1 87 217 1 116 7.53e-42 147
Msa0132250 MtrunA17_Chr4g0001121 64.835 91 24 2 216 300 10 98 2.90e-31 112
Msa0132250 MtrunA17_Chr2g0331261 36.481 233 128 6 69 287 15 241 4.81e-31 116
Msa0132250 MtrunA17_Chr1g0146161 35.841 226 126 7 74 287 19 237 2.44e-28 109
Msa0132250 MtrunA17_Chr8g0340161 37.089 213 113 7 78 275 23 229 7.52e-28 108
Msa0132250 MtrunA17_Chr1g0146171 35.981 214 118 7 74 275 19 225 1.64e-27 107
Msa0132250 MtrunA17_Chr1g0212671 33.333 228 132 7 78 291 25 246 9.44e-23 94.7
Msa0132250 MtrunA17_Chr5g0399341 33.333 234 134 5 78 298 23 247 2.70e-21 90.5
Msa0132250 MtrunA17_Chr2g0326621 30.677 251 139 7 71 287 37 286 2.29e-19 86.3
Msa0132250 MtrunA17_Chr4g0030861 32.870 216 123 8 79 279 26 234 6.82e-19 84.0
Msa0132250 MtrunA17_Chr7g0222791 31.330 233 130 8 78 291 25 246 1.84e-18 82.8
Msa0132250 MtrunA17_Chr6g0478191 28.571 224 149 4 78 293 25 245 6.36e-18 81.6
Msa0132250 MtrunA17_Chr7g0264961 30.804 224 144 6 78 292 25 246 7.24e-17 78.6
Msa0132250 MtrunA17_Chr1g0188771 30.357 224 145 5 78 293 25 245 8.32e-17 78.6
Msa0132250 MtrunA17_Chr3g0145671 29.060 234 148 7 66 282 29 261 3.60e-16 77.0
Msa0132250 MtrunA17_Chr4g0020781 32.967 182 102 5 126 299 36 205 2.24e-15 73.6
Msa0132250 MtrunA17_Chr8g0386961 29.646 226 139 8 78 285 56 279 1.63e-13 69.7
Msa0132250 MtrunA17_Chr7g0264111 27.092 251 157 8 71 299 36 282 1.82e-13 69.3
Msa0132250 MtrunA17_Chr4g0006001 29.333 225 140 9 78 285 60 282 1.96e-13 69.3
Msa0132250 MtrunA17_Chr3g0113211 26.126 222 147 6 78 282 59 280 3.32e-13 68.6
Msa0132250 MtrunA17_Chr5g0436701 26.667 225 147 6 78 285 59 282 6.53e-13 67.8
Msa0132250 MtrunA17_Chr5g0397391 28.319 226 142 8 78 285 56 279 8.15e-13 67.4
Msa0132250 MtrunA17_Chr4g0029071 25.591 254 157 7 66 287 31 284 2.09e-12 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0132250 AT4G10380.1 71.174 281 71 3 25 300 29 304 1.01e-143 407
Msa0132250 AT1G80760.1 56.550 313 101 4 9 300 7 305 3.90e-111 324
Msa0132250 AT5G37820.1 43.173 271 138 4 35 300 11 270 8.56e-69 216
Msa0132250 AT4G19030.1 49.167 240 108 3 69 300 50 283 1.30e-68 215
Msa0132250 AT5G37820.2 43.866 269 139 4 35 300 47 306 2.43e-68 216
Msa0132250 AT5G37810.1 42.647 272 143 5 35 300 6 270 8.50e-68 213
Msa0132250 AT4G18910.1 46.694 242 111 3 69 300 47 280 1.25e-65 207
Msa0132250 AT1G31885.1 50.673 223 102 2 69 283 38 260 4.29e-65 207
Msa0132250 AT1G31885.2 49.515 206 96 2 86 283 1 206 1.06e-57 186
Msa0132250 AT2G34390.1 43.777 233 117 4 69 291 43 271 1.92e-52 174
Msa0132250 AT2G34390.2 43.172 227 115 4 75 291 7 229 1.60e-50 167
Msa0132250 AT3G06100.1 44.762 210 113 3 78 285 50 258 2.79e-47 160
Msa0132250 AT3G06100.2 44.340 212 115 3 78 287 2 212 3.53e-47 158
Msa0132250 AT4G19030.3 49.143 175 81 2 69 235 50 224 2.84e-45 153
Msa0132250 AT4G18910.2 48.315 178 80 2 69 236 47 222 3.38e-44 150
Msa0132250 AT4G19030.2 46.795 156 75 2 69 216 50 205 1.61e-34 125
Msa0132250 AT2G34390.3 41.573 178 91 3 69 236 43 217 7.34e-32 118
Msa0132250 AT3G16240.1 38.028 213 111 8 78 275 23 229 8.02e-29 111
Msa0132250 AT2G29870.1 50.980 102 49 1 190 291 22 122 4.56e-26 100
Msa0132250 AT2G34390.4 40.625 160 82 3 69 218 43 199 2.46e-25 101
Msa0132250 AT5G47450.1 36.652 221 121 7 69 275 14 229 2.02e-24 99.4
Msa0132250 AT4G17340.1 35.849 212 117 6 78 275 23 229 4.85e-24 98.6
Msa0132250 AT2G25810.1 36.842 209 113 7 79 275 24 225 5.16e-24 98.2
Msa0132250 AT3G06100.3 39.855 138 82 1 149 285 40 177 2.76e-22 92.4
Msa0132250 AT3G26520.1 33.186 226 133 7 78 290 26 246 7.85e-22 92.4
Msa0132250 AT2G36830.1 34.348 230 129 8 78 292 25 247 1.95e-21 91.3
Msa0132250 AT4G01470.1 31.760 233 129 8 78 291 25 246 3.96e-19 85.1
Msa0132250 AT1G17810.1 29.258 229 148 5 78 293 28 255 2.11e-17 80.5
Msa0132250 AT1G31885.3 50.000 80 40 0 69 148 38 117 2.29e-17 77.8
Msa0132250 AT4G35100.1 29.600 250 156 7 66 299 30 275 7.55e-17 79.3
Msa0132250 AT4G35100.2 29.600 250 156 7 66 299 30 275 7.55e-17 79.3
Msa0132250 AT2G16850.1 29.796 245 152 7 71 299 33 273 9.37e-16 76.3
Msa0132250 AT1G73190.1 28.509 228 147 5 78 292 28 252 1.08e-15 75.9
Msa0132250 AT5G60660.1 26.383 235 147 7 72 282 37 269 3.15e-14 72.0
Msa0132250 AT3G53420.1 26.996 263 165 8 59 297 24 283 4.16e-14 71.6
Msa0132250 AT3G53420.2 26.996 263 165 8 59 297 24 283 4.16e-14 71.6
Msa0132250 AT2G37170.1 26.820 261 164 8 61 297 24 281 5.03e-14 71.2
Msa0132250 AT2G37170.2 26.641 259 167 7 61 297 67 324 9.12e-14 71.2
Msa0132250 AT3G54820.1 26.724 232 146 7 64 272 28 258 6.11e-13 68.2
Msa0132250 AT2G37180.1 25.869 259 169 7 61 297 24 281 8.70e-13 67.8
Msa0132250 AT2G45960.1 28.000 225 143 8 78 285 56 278 1.19e-11 64.3
Msa0132250 AT1G01620.1 27.488 211 137 6 78 272 56 266 1.85e-11 63.9
Msa0132250 AT3G61430.2 27.556 225 144 8 78 285 56 278 2.24e-11 63.5
Msa0132250 AT3G61430.1 27.556 225 144 8 78 285 56 278 2.24e-11 63.5
Msa0132250 AT3G47440.1 27.897 233 141 6 78 296 27 246 2.43e-11 63.2
Msa0132250 AT4G00430.1 27.232 224 146 7 78 285 57 279 2.75e-11 63.5
Msa0132250 AT4G23400.1 27.556 225 144 8 78 285 57 279 2.80e-11 63.5

Find 130 sgRNAs with CRISPR-Local

Find 316 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGCATCTCTGGATATATT+TGG 0.216287 1_3:-63236753 Msa0132250:CDS
AATCTCTCCAACTTCGAAAT+TGG 0.246759 1_3:-63241576 Msa0132250:five_prime_UTR
GGTGTCCCTGACTCCGATTC+TGG 0.274084 1_3:+63241194 None:intergenic
TTGTATTTGTTGTTCACTAT+TGG 0.275818 1_3:+63237810 None:intergenic
ATAGATTATAAAATTGAGTT+TGG 0.281086 1_3:+63241735 None:intergenic
AAAGAGTACAGTTACATAAT+TGG 0.297226 1_3:+63241366 None:intergenic
TTCGAAGTTGGAGAGATTAT+AGG 0.312909 1_3:+63241581 None:intergenic
CAATGGAGTAGAAACTCTTA+TGG 0.319532 1_3:-63237790 Msa0132250:CDS
CTTGGTGCTCTAGCTGGTTC+TGG 0.336743 1_3:-63236719 Msa0132250:CDS
ATAAATTTGTTTCTAAGGTA+TGG 0.361143 1_3:+63241398 None:intergenic
TTGCTCAACATTCTTATCTC+AGG 0.361959 1_3:-63237196 Msa0132250:CDS
GAACATGTGCCCACGGGAAA+TGG 0.378646 1_3:+63237658 None:intergenic
GGTTAATGTGGATTATCTAA+TGG 0.381324 1_3:-63236443 Msa0132250:three_prime_UTR
ATGAATCCGGTGCGCACCTT+AGG 0.389490 1_3:-63236800 Msa0132250:CDS
GTTTACCATCCTTTCCTCTC+TGG 0.391500 1_3:-63237576 Msa0132250:CDS
TGCAATGCAGGTTGGTGAAT+TGG 0.400837 1_3:-63237245 Msa0132250:intron
TATGTAGTTGTTGTTAAGGT+TGG 0.402677 1_3:+63241516 None:intergenic
ATGTTCATTATTCTATCAAT+TGG 0.408502 1_3:-63237738 Msa0132250:CDS
ATCACTCACCATTGCCTTCG+CGG 0.410120 1_3:-63237688 Msa0132250:CDS
TAAGGTGCGCACCGGATTCA+TGG 0.410875 1_3:+63236802 None:intergenic
CAGGAAATTACAAGCATCTC+TGG 0.418374 1_3:-63236763 Msa0132250:CDS
TAATTTCCTGCTGCAACGGC+TGG 0.421426 1_3:+63236776 None:intergenic
CCGACGCTTGGTGCTCTAGC+TGG 0.422426 1_3:-63236725 Msa0132250:CDS
AAAATGCCAGAATCGGAGTC+AGG 0.425265 1_3:-63241200 Msa0132250:CDS
AATGGCCCTCCGGGAGTATC+CGG 0.425506 1_3:+63241146 None:intergenic
TTTGGAAAGTGCTATGAATA+TGG 0.427152 1_3:+63241753 None:intergenic
TGGATATGTAGTTGTTGTTA+AGG 0.429772 1_3:+63241512 None:intergenic
ATTCTTATAGATGAGAATAA+TGG 0.431504 1_3:+63241128 None:intergenic
TAATGAACATATGAAAAGAT+TGG 0.431700 1_3:+63241492 None:intergenic
CGGAGCTGAACCACCTCAAC+CGG 0.432157 1_3:-63236666 Msa0132250:CDS
TGCTGCAACGGCTGGACCTA+AGG 0.434795 1_3:+63236784 None:intergenic
TCAACAACTTATGGGGATGA+TGG 0.436919 1_3:+63241670 None:intergenic
CTCGATTACACTCGATTACA+AGG 0.438875 1_3:+63236528 None:intergenic
AAGATGAGGTATTTAATTGT+GGG 0.439925 1_3:+63241459 None:intergenic
CAACGTGAGTCATTTGCAAT+GGG 0.443310 1_3:-63241092 Msa0132250:CDS
CGTTATCACGAAGCTTGATA+AGG 0.443454 1_3:+63236687 None:intergenic
TTCATGGAACCACCGCTTGT+TGG 0.443747 1_3:+63236818 None:intergenic
AAAGATGAGGTATTTAATTG+TGG 0.444642 1_3:+63241458 None:intergenic
CTATCAATTGGTCATATCTC+AGG 0.449732 1_3:-63237726 Msa0132250:CDS
GGAGCTTTCGTCGCTAGAGC+TGG 0.454976 1_3:-63236640 Msa0132250:exon
TTGTGATCCAATTTCGAAGT+TGG 0.460953 1_3:+63241569 None:intergenic
CTTATCAAGCTTCGTGATAA+CGG 0.463299 1_3:-63236686 Msa0132250:CDS
ATGGGAAATGCAGCTTGTGC+TGG 0.464614 1_3:-63237771 Msa0132250:CDS
TAAGAGAAAACTTGTGATGA+AGG 0.468893 1_3:+63241546 None:intergenic
AATGGAGTAGAAACTCTTAT+GGG 0.469616 1_3:-63237789 Msa0132250:CDS
TTTAGAAAATTATGTAGAGT+TGG 0.474450 1_3:-63241290 Msa0132250:five_prime_UTR
CTAAGGTATGGAAAGAAAGA+AGG 0.477970 1_3:+63241410 None:intergenic
GTTATCACGAAGCTTGATAA+GGG 0.481400 1_3:+63236688 None:intergenic
CTGAACCACCTCAACCGGTT+AGG 0.481691 1_3:-63236661 Msa0132250:CDS
TGTGTCATGAAAGCTTTGTT+TGG 0.490520 1_3:-63236464 Msa0132250:three_prime_UTR
CTTGTAATTTCCTGCTGCAA+CGG 0.491803 1_3:+63236772 None:intergenic
TAGCGACGAAAGCTCCTAAC+CGG 0.492246 1_3:+63236647 None:intergenic
AGCCTAATTAAATAAAATGT+GGG 0.492607 1_3:-63236555 Msa0132250:three_prime_UTR
GCCATGGTTTGTATTATGAA+TGG 0.492916 1_3:-63236410 Msa0132250:three_prime_UTR
CGGCGTTTCGCCATTTCCCG+TGG 0.493217 1_3:-63237668 Msa0132250:CDS
ACTTGTGCTGCAATGTAGGC+TGG 0.494118 1_3:+63237636 None:intergenic
GAGGTGCAACATGTGTATGC+CGG 0.494142 1_3:-63241069 Msa0132250:CDS
ACAGATTGGAGCAGAGTTTG+TGG 0.494648 1_3:-63237868 Msa0132250:intron
TATTTGGTGGCACCGACGCT+TGG 0.495981 1_3:-63236737 Msa0132250:CDS
TTAGGTCCAGCCGTTGCAGC+AGG 0.497485 1_3:-63236782 Msa0132250:CDS
AAGCGGTGGTTCCATGAATC+CGG 0.497866 1_3:-63236813 Msa0132250:CDS
CGAAAGCTCCTAACCGGTTG+AGG 0.499069 1_3:+63236653 None:intergenic
TACCATCCTTTCCTCTCTGG+TGG 0.500750 1_3:-63237573 Msa0132250:CDS
TCTAGCTAAATCTACAAACC+TGG 0.501272 1_3:-63241436 Msa0132250:five_prime_UTR
TGCTCAACATTCTTATCTCA+GGG 0.505305 1_3:-63237195 Msa0132250:intron
AGCGTCGGTGCCGGCGACAC+CGG 0.511138 1_3:-63241165 Msa0132250:CDS
AGGTGCAACATGTGTATGCC+GGG 0.512535 1_3:-63241068 Msa0132250:CDS
CAGATTGGAGCAGAGTTTGT+GGG 0.519331 1_3:-63237867 Msa0132250:CDS
GAATTGGCTGGTATTGCTGT+TGG 0.524769 1_3:-63237229 Msa0132250:CDS
GATGAGAATAATGGCCCTCC+GGG 0.524874 1_3:+63241137 None:intergenic
AGATGAGAATAATGGCCCTC+CGG 0.525358 1_3:+63241136 None:intergenic
AAAGCTTTGTTTGGTTAATG+TGG 0.526294 1_3:-63236455 Msa0132250:three_prime_UTR
AAGTGTCTCTCTTTCTCAGA+AGG 0.536928 1_3:-63240994 Msa0132250:intron
CCGGGAGTATCCGGTGTCGC+CGG 0.538656 1_3:+63241155 None:intergenic
AGAGACTTGTGCTGCAATGT+AGG 0.541162 1_3:+63237632 None:intergenic
AGTTGTTGTTAAGGTTGGAA+TGG 0.541398 1_3:+63241521 None:intergenic
ACCGACGGCAGCGTCGGTGC+CGG 0.542854 1_3:-63241174 Msa0132250:CDS
GAGCCTAATTAAATAAAATG+TGG 0.546465 1_3:-63236556 Msa0132250:three_prime_UTR
TCAACGTGAGTCATTTGCAA+TGG 0.548537 1_3:-63241093 Msa0132250:CDS
TGCGCTTGTTATGCGCTCAA+AGG 0.549531 1_3:-63237600 Msa0132250:CDS
TAGATTTAGCTAGAAAGATG+AGG 0.551425 1_3:+63241445 None:intergenic
AAGGTGGATGAGAGGACTAA+TGG 0.556098 1_3:+63241631 None:intergenic
AGTACAGTTACATAATTGGA+TGG 0.558005 1_3:+63241370 None:intergenic
GGCGTTTCGCCATTTCCCGT+GGG 0.558137 1_3:-63237667 Msa0132250:CDS
TAATAATGTGTTCTGTTGTG+TGG 0.558749 1_3:-63236374 Msa0132250:three_prime_UTR
GAGCTTGAAATATGAGAAGT+GGG 0.558919 1_3:+63236595 None:intergenic
GCGAAACGCCGCGAAGGCAA+TGG 0.561703 1_3:+63237680 None:intergenic
TTCTGAGAAAGAGAGACACT+TGG 0.567115 1_3:+63240996 None:intergenic
GCTTGTCCAACGCTAACAGT+AGG 0.567496 1_3:+63237540 None:intergenic
GCCGGCACCGACGCTGCCGT+CGG 0.571291 1_3:+63241173 None:intergenic
CTAATATATGCAGCAACAGC+AGG 0.571804 1_3:-63237834 Msa0132250:CDS
AAATGCCAGAATCGGAGTCA+GGG 0.577507 1_3:-63241199 Msa0132250:CDS
CCGGCGACACCGGATACTCC+CGG 0.580871 1_3:-63241155 Msa0132250:CDS
TAAGGTATGGAAAGAAAGAA+GGG 0.581499 1_3:+63241411 None:intergenic
CTTGTCCAACGCTAACAGTA+GGG 0.582924 1_3:+63237541 None:intergenic
GCGAAGGCAATGGTGAGTGA+TGG 0.584999 1_3:+63237690 None:intergenic
AGAGCTTGAAATATGAGAAG+TGG 0.588128 1_3:+63236594 None:intergenic
GCATCTCTGGATATATTTGG+TGG 0.589329 1_3:-63236750 Msa0132250:CDS
CTAGCTAAATCTACAAACCT+GGG 0.589533 1_3:-63241435 Msa0132250:five_prime_UTR
GAAATGGCGAAACGCCGCGA+AGG 0.592464 1_3:+63237674 None:intergenic
ACCATTCATAATACAAACCA+TGG 0.595763 1_3:+63236409 None:intergenic
ATGATGGAGACGAAGTGATG+AGG 0.595924 1_3:+63241686 None:intergenic
ATGCAGGTTGGTGAATTGGC+TGG 0.597369 1_3:-63237241 Msa0132250:intron
ATGAATTGAACGTACCGCAC+GGG 0.604011 1_3:+63237452 None:intergenic
CGTGGTTGAAGGTGGATGAG+AGG 0.608502 1_3:+63241623 None:intergenic
GCTGGACCTAAGGTGCGCAC+CGG 0.614709 1_3:+63236794 None:intergenic
TAGGCTGGAACATGTGCCCA+CGG 0.616186 1_3:+63237651 None:intergenic
ACTGTCCCTACTGTTAGCGT+TGG 0.619097 1_3:-63237546 Msa0132250:CDS
ATAGTGAACAACAAATACAA+TGG 0.626790 1_3:-63237807 Msa0132250:CDS
CCAGCTAGAGCACCAAGCGT+CGG 0.629619 1_3:+63236725 None:intergenic
AGGCTGGAACATGTGCCCAC+GGG 0.634760 1_3:+63237652 None:intergenic
CACCACCAGAGAGGAAAGGA+TGG 0.636860 1_3:+63237571 None:intergenic
AGGGACACCGACGGCAGCGT+CGG 0.638264 1_3:-63241180 Msa0132250:CDS
GCTTGAAATATGAGAAGTGG+GGG 0.639472 1_3:+63236597 None:intergenic
GTGAGTCATTTGCAATGGGG+AGG 0.644380 1_3:-63241088 Msa0132250:CDS
GCCTAATTAAATAAAATGTG+GGG 0.644829 1_3:-63236554 Msa0132250:three_prime_UTR
GTGACACCACCAGAGAGGAA+AGG 0.645575 1_3:+63237567 None:intergenic
TGGAAAGAAAGAAGGGACCC+AGG 0.648546 1_3:+63241418 None:intergenic
TGGATGAGAGGACTAATGGT+CGG 0.655282 1_3:+63241635 None:intergenic
AAGCTCCTAACCGGTTGAGG+TGG 0.657778 1_3:+63236656 None:intergenic
AATGCTAGGCCAACAAGCGG+TGG 0.661522 1_3:-63236827 Msa0132250:intron
AGCTTGAAATATGAGAAGTG+GGG 0.669139 1_3:+63236596 None:intergenic
ATCGGAGTCAGGGACACCGA+CGG 0.669278 1_3:-63241189 Msa0132250:CDS
GTAAATGCTAGGCCAACAAG+CGG 0.699062 1_3:-63236830 Msa0132250:intron
CGACACCGGATACTCCCGGA+GGG 0.703914 1_3:-63241151 Msa0132250:CDS
AACGTGAGTCATTTGCAATG+GGG 0.719998 1_3:-63241091 Msa0132250:CDS
GGACAGTGACACCACCAGAG+AGG 0.725765 1_3:+63237562 None:intergenic
GCGACACCGGATACTCCCGG+AGG 0.726349 1_3:-63241152 Msa0132250:CDS
TGCAACATGTGTATGCCGGG+TGG 0.746549 1_3:-63241065 Msa0132250:CDS
TGTTGCTACTGATTCCCGTG+CGG 0.778451 1_3:-63237466 Msa0132250:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATAAATTTGTTTCTA+AGG + chr1_3:63236770-63236789 None:intergenic 10.0%
!! TAGTTAATTTAATTAACAAT+TGG - chr1_3:63237473-63237492 Msa0132250:CDS 10.0%
!! AGTTAATTTAATTAACAATT+GGG - chr1_3:63237474-63237493 Msa0132250:CDS 10.0%
!!! TAATAAAAAAATTGTTTTCA+AGG + chr1_3:63237831-63237850 None:intergenic 10.0%
!! TTATATTGAAATGAAATATA+TGG - chr1_3:63238054-63238073 Msa0132250:intron 10.0%
!!! ATTTCAATATATAATTTTGT+CGG - chr1_3:63238106-63238125 Msa0132250:intron 10.0%
!! TAAAATAAAAAAGATTAATG+CGG - chr1_3:63238481-63238500 Msa0132250:intron 10.0%
!!! TACTTTTCTTTTTTAAAATA+CGG + chr1_3:63238911-63238930 None:intergenic 10.0%
!! ATAGATTATAAAATTGAGTT+TGG + chr1_3:63236428-63236447 None:intergenic 15.0%
!!! AAATAGATAGCATTTTTTTT+AGG - chr1_3:63237380-63237399 Msa0132250:intron 15.0%
!!! AATAGATAGCATTTTTTTTA+GGG - chr1_3:63237381-63237400 Msa0132250:intron 15.0%
!! AAAACTATTCAATATACTTT+CGG + chr1_3:63238393-63238412 None:intergenic 15.0%
!!! TAAGCAAAAATTGATTTTTT+AGG - chr1_3:63238542-63238561 Msa0132250:intron 15.0%
!!! TTTTTAGATTCATTCAATAA+AGG + chr1_3:63238615-63238634 None:intergenic 15.0%
!!! TTTTAAAAGTTGATTAAATG+AGG - chr1_3:63239022-63239041 Msa0132250:intron 15.0%
!!! TTTAAAAGTTGATTAAATGA+GGG - chr1_3:63239023-63239042 Msa0132250:intron 15.0%
!! TGATAAAAACTTACTTTATT+TGG + chr1_3:63239182-63239201 None:intergenic 15.0%
!!! GAAATTACATAGATTTTTTT+TGG - chr1_3:63239676-63239695 Msa0132250:intron 15.0%
!!! TATAAGTTGTTTGTTTTTTT+TGG + chr1_3:63239850-63239869 None:intergenic 15.0%
!! ATTCAGACAAAATTTATATT+CGG + chr1_3:63241166-63241185 None:intergenic 15.0%
!! TAATGAACATATGAAAAGAT+TGG + chr1_3:63236671-63236690 None:intergenic 20.0%
!!! ATAAATTTGTTTCTAAGGTA+TGG + chr1_3:63236765-63236784 None:intergenic 20.0%
!!! TTTTGTTTTTGAGAGTTTTA+AGG + chr1_3:63236823-63236842 None:intergenic 20.0%
!! TTTAGAAAATTATGTAGAGT+TGG - chr1_3:63236870-63236889 Msa0132250:intron 20.0%
!! TAAAAAAAAAATGCCAGAAT+CGG - chr1_3:63236953-63236972 Msa0132250:intron 20.0%
!! ATTCTTATAGATGAGAATAA+TGG + chr1_3:63237035-63237054 None:intergenic 20.0%
!!! TCTATTTGATATTTTAGTCA+AGG + chr1_3:63237367-63237386 None:intergenic 20.0%
!!! GGAATGTATATTTTTTTTGA+AGG + chr1_3:63237746-63237765 None:intergenic 20.0%
!!! AGGAAATAAATTTTATTTGC+TGG + chr1_3:63237767-63237786 None:intergenic 20.0%
!!! TTTTTGTTTGCTTCTTTTTT+AGG + chr1_3:63237787-63237806 None:intergenic 20.0%
!! TACATATACTATATTACACA+CGG + chr1_3:63237864-63237883 None:intergenic 20.0%
!!! AAATGTATTTTGACATAGAT+GGG - chr1_3:63238134-63238153 Msa0132250:intron 20.0%
!! TTTCATTGATAAACAAAAGA+TGG - chr1_3:63238174-63238193 Msa0132250:intron 20.0%
!!! ACATGTTTGATATTTTCTTA+GGG + chr1_3:63238979-63238998 None:intergenic 20.0%
!!! AACATGTTTGATATTTTCTT+AGG + chr1_3:63238980-63238999 None:intergenic 20.0%
!!! TTAAAAGTTGATTAAATGAG+GGG - chr1_3:63239024-63239043 Msa0132250:intron 20.0%
!!! TAGGATTTATTTTTTTTGAG+AGG - chr1_3:63239347-63239366 Msa0132250:intron 20.0%
!! ATTGTTTAAACAACTAAAAG+TGG + chr1_3:63239570-63239589 None:intergenic 20.0%
!! ACAAAATTAAATCAACTACA+TGG + chr1_3:63239932-63239951 None:intergenic 20.0%
!!! TGATTTAATTTTGTAAGCTT+TGG - chr1_3:63239938-63239957 Msa0132250:intron 20.0%
!!! GATTTAATTTTGTAAGCTTT+GGG - chr1_3:63239939-63239958 Msa0132250:intron 20.0%
!!! ATTTAATTTTGTAAGCTTTG+GGG - chr1_3:63239940-63239959 Msa0132250:intron 20.0%
!!! TTTTTCCAAAAATCTACATT+TGG - chr1_3:63240107-63240126 Msa0132250:intron 20.0%
!!! TACTTTTCCTTTTTCATTAA+GGG + chr1_3:63240237-63240256 None:intergenic 20.0%
!!! TTACTTTTCCTTTTTCATTA+AGG + chr1_3:63240238-63240257 None:intergenic 20.0%
!! ATGTTCATTATTCTATCAAT+TGG - chr1_3:63240422-63240441 Msa0132250:intron 20.0%
!!! AAATTTCAAGTTCTCTAAAT+TGG - chr1_3:63240808-63240827 Msa0132250:intron 20.0%
!!! AATTTCAAGTTCTCTAAATT+GGG - chr1_3:63240809-63240828 Msa0132250:intron 20.0%
!!! ATTGAGTTTTTTATGAGTTA+AGG - chr1_3:63240860-63240879 Msa0132250:intron 20.0%
!!! TGATGTTATTGATTTGTATT+TGG - chr1_3:63241010-63241029 Msa0132250:CDS 20.0%
!!! TTGTATTTGGTTTTCATAAT+AGG - chr1_3:63241023-63241042 Msa0132250:CDS 20.0%
!!! ATTCAATGATAATGTTTCAA+AGG - chr1_3:63241076-63241095 Msa0132250:CDS 20.0%
!! AGCCTAATTAAATAAAATGT+GGG - chr1_3:63241605-63241624 Msa0132250:five_prime_UTR 20.0%
!! TTGATTTTCAACAACTTATG+GGG + chr1_3:63236500-63236519 None:intergenic 25.0%
!!! GTTGATTTTCAACAACTTAT+GGG + chr1_3:63236501-63236520 None:intergenic 25.0%
!!! AGTTGATTTTCAACAACTTA+TGG + chr1_3:63236502-63236521 None:intergenic 25.0%
! AGAGATTATAGGAAAAAGAA+AGG + chr1_3:63236571-63236590 None:intergenic 25.0%
!! AAGATGAGGTATTTAATTGT+GGG + chr1_3:63236704-63236723 None:intergenic 25.0%
!! AAAGATGAGGTATTTAATTG+TGG + chr1_3:63236705-63236724 None:intergenic 25.0%
!! AAAGAGTACAGTTACATAAT+TGG + chr1_3:63236797-63236816 None:intergenic 25.0%
!! ATCTCTCTCTTTATGATTTT+TGG - chr1_3:63237193-63237212 Msa0132250:intron 25.0%
!!! ACCAAATGGAATTAATTTGT+GGG + chr1_3:63237271-63237290 None:intergenic 25.0%
!! AACCAAATGGAATTAATTTG+TGG + chr1_3:63237272-63237291 None:intergenic 25.0%
! GATACAAATACAAAAACAAG+TGG + chr1_3:63237505-63237524 None:intergenic 25.0%
!!! TTTTTTTTGAAGGAATTTGC+TGG + chr1_3:63237736-63237755 None:intergenic 25.0%
! CTATGTCAAAATACATTTGA+AGG + chr1_3:63238132-63238151 None:intergenic 25.0%
!!! CAAATGTATTTTGACATAGA+TGG - chr1_3:63238133-63238152 Msa0132250:intron 25.0%
!! TTTTTGCTTATAATAGTGAC+CGG + chr1_3:63238532-63238551 None:intergenic 25.0%
!!! AGGAGGTATATATTTTACTA+AGG - chr1_3:63238716-63238735 Msa0132250:intron 25.0%
!!! GGAGGTATATATTTTACTAA+GGG - chr1_3:63238717-63238736 Msa0132250:intron 25.0%
! CATAAATAGAATTTCTGTCT+TGG + chr1_3:63239251-63239270 None:intergenic 25.0%
! GATTGACACGATTAAATAAA+GGG + chr1_3:63239314-63239333 None:intergenic 25.0%
! AGATTGACACGATTAAATAA+AGG + chr1_3:63239315-63239334 None:intergenic 25.0%
!!! TATTTTTTTTGAGAGGAACT+TGG - chr1_3:63239354-63239373 Msa0132250:intron 25.0%
!!! ATTTTTTTTGAGAGGAACTT+GGG - chr1_3:63239355-63239374 Msa0132250:intron 25.0%
!!! GGTTCGACTTTTTTTTTTAA+GGG - chr1_3:63239376-63239395 Msa0132250:intron 25.0%
!!! TTTTTTTAAGGGAATGAACT+TGG - chr1_3:63239387-63239406 Msa0132250:intron 25.0%
!!! TTTTTTAAGGGAATGAACTT+GGG - chr1_3:63239388-63239407 Msa0132250:intron 25.0%
!! AAACAGTCTCAAAGTATTTA+TGG + chr1_3:63239795-63239814 None:intergenic 25.0%
!!! TTTTTGGAAAAATCCACATT+TGG + chr1_3:63240099-63240118 None:intergenic 25.0%
!! ATGTTCCAAATGTAGATTTT+TGG + chr1_3:63240115-63240134 None:intergenic 25.0%
!!! ACTTTTCCTTTTTCATTAAG+GGG + chr1_3:63240236-63240255 None:intergenic 25.0%
!!! TTGTATTTGTTGTTCACTAT+TGG + chr1_3:63240353-63240372 None:intergenic 25.0%
! ATAGTGAACAACAAATACAA+TGG - chr1_3:63240353-63240372 Msa0132250:intron 25.0%
!!! GTTAAGGTTGTTTTAATTCA+TGG - chr1_3:63240876-63240895 Msa0132250:intron 25.0%
!!! TATTTTTGTATATGCAATGC+AGG - chr1_3:63240903-63240922 Msa0132250:intron 25.0%
! TAACATCAATTCATGAGAAA+AGG + chr1_3:63240998-63241017 None:intergenic 25.0%
! TGTCAAATGTTATATTCAGT+TGG - chr1_3:63241052-63241071 Msa0132250:CDS 25.0%
! ATCATGAAAAACTATTCCAA+AGG - chr1_3:63241104-63241123 Msa0132250:CDS 25.0%
!! AATTTTGTCTGAATAATGAC+AGG - chr1_3:63241172-63241191 Msa0132250:CDS 25.0%
! GAGCCTAATTAAATAAAATG+TGG - chr1_3:63241604-63241623 Msa0132250:five_prime_UTR 25.0%
! GCCTAATTAAATAAAATGTG+GGG - chr1_3:63241606-63241625 Msa0132250:five_prime_UTR 25.0%
!!! TCCCCACATTTTATTTAATT+AGG + chr1_3:63241610-63241629 None:intergenic 25.0%
!! TTTGGAAAGTGCTATGAATA+TGG + chr1_3:63236410-63236429 None:intergenic 30.0%
AAAAGAAAGGCAAAAAAACG+TGG + chr1_3:63236558-63236577 None:intergenic 30.0%
TAAGAGAAAACTTGTGATGA+AGG + chr1_3:63236617-63236636 None:intergenic 30.0%
! TATGTAGTTGTTGTTAAGGT+TGG + chr1_3:63236647-63236666 None:intergenic 30.0%
! TGGATATGTAGTTGTTGTTA+AGG + chr1_3:63236651-63236670 None:intergenic 30.0%
TAGATTTAGCTAGAAAGATG+AGG + chr1_3:63236718-63236737 None:intergenic 30.0%
TAAGGTATGGAAAGAAAGAA+GGG + chr1_3:63236752-63236771 None:intergenic 30.0%
AGTACAGTTACATAATTGGA+TGG + chr1_3:63236793-63236812 None:intergenic 30.0%
!! AAATGGAATTAATTTGTGGG+CGG + chr1_3:63237268-63237287 None:intergenic 30.0%
GTCAGATTTAACTGTTTCAT+GGG - chr1_3:63237305-63237324 Msa0132250:intron 30.0%
TAAATAGTATTGTTTGCACG+TGG - chr1_3:63237574-63237593 Msa0132250:CDS 30.0%
!!! TTTTGACATAGATGGGTTAT+TGG - chr1_3:63238141-63238160 Msa0132250:intron 30.0%
CTAGTAACATGGGTTTATTA+GGG + chr1_3:63238318-63238337 None:intergenic 30.0%
ACTAGTAACATGGGTTTATT+AGG + chr1_3:63238319-63238338 None:intergenic 30.0%
ACAAATGCAACTAGTAACAT+GGG + chr1_3:63238328-63238347 None:intergenic 30.0%
AACAAATGCAACTAGTAACA+TGG + chr1_3:63238329-63238348 None:intergenic 30.0%
! CTTTTGCTTATAATAGTGAC+CGG - chr1_3:63238640-63238659 Msa0132250:intron 30.0%
TATATGACAGCCAAAATTTC+AGG - chr1_3:63238669-63238688 Msa0132250:intron 30.0%
ATATGACAGCCAAAATTTCA+GGG - chr1_3:63238670-63238689 Msa0132250:intron 30.0%
! GACATGTTTTCTCAATATCA+AGG - chr1_3:63238696-63238715 Msa0132250:intron 30.0%
! ATGTTTTCTCAATATCAAGG+AGG - chr1_3:63238699-63238718 Msa0132250:intron 30.0%
!! TATATTTTACTAAGGGTGTG+TGG - chr1_3:63238724-63238743 Msa0132250:intron 30.0%
! ATATTTTCTTAGGGTGTGTT+TGG + chr1_3:63238970-63238989 None:intergenic 30.0%
GAGCTTGACTCATTTATTTA+AGG - chr1_3:63239118-63239137 Msa0132250:intron 30.0%
!! AAATAAAGGGTCTTGTTAAC+CGG + chr1_3:63239301-63239320 None:intergenic 30.0%
!!! GGGTTCGACTTTTTTTTTTA+AGG - chr1_3:63239375-63239394 Msa0132250:intron 30.0%
TTAGGTTAACAAGTGTCATA+AGG - chr1_3:63239483-63239502 Msa0132250:intron 30.0%
TAGGTTAACAAGTGTCATAA+GGG - chr1_3:63239484-63239503 Msa0132250:intron 30.0%
TTCAAAAGTTCTCCAAACAA+AGG - chr1_3:63239902-63239921 Msa0132250:intron 30.0%
GGATTAGAATAATGTGACAA+TGG + chr1_3:63240214-63240233 None:intergenic 30.0%
!! CTTTTCCTTTTTCATTAAGG+GGG + chr1_3:63240235-63240254 None:intergenic 30.0%
TGTTAAAATGTGTCACAGAT+TGG - chr1_3:63240278-63240297 Msa0132250:intron 30.0%
AATGGAGTAGAAACTCTTAT+GGG - chr1_3:63240371-63240390 Msa0132250:intron 30.0%
TTAGAGAACTTGAAATTTCG+CGG + chr1_3:63240806-63240825 None:intergenic 30.0%
GTCATGTCATTAAAAACCTT+TGG + chr1_3:63241123-63241142 None:intergenic 30.0%
TAAGATTGTGAGTAATGTTG+TGG - chr1_3:63241305-63241324 Msa0132250:five_prime_UTR 30.0%
!! AAAGCTTTGTTTGGTTAATG+TGG - chr1_3:63241705-63241724 Msa0132250:five_prime_UTR 30.0%
! GGTTAATGTGGATTATCTAA+TGG - chr1_3:63241717-63241736 Msa0132250:five_prime_UTR 30.0%
ACCATTCATAATACAAACCA+TGG + chr1_3:63241754-63241773 None:intergenic 30.0%
TTCGAAGTTGGAGAGATTAT+AGG + chr1_3:63236582-63236601 None:intergenic 35.0%
AATCTCTCCAACTTCGAAAT+TGG - chr1_3:63236584-63236603 Msa0132250:three_prime_UTR 35.0%
TTGTGATCCAATTTCGAAGT+TGG + chr1_3:63236594-63236613 None:intergenic 35.0%
! AGTTGTTGTTAAGGTTGGAA+TGG + chr1_3:63236642-63236661 None:intergenic 35.0%
TCTAGCTAAATCTACAAACC+TGG - chr1_3:63236724-63236743 Msa0132250:CDS 35.0%
CTAGCTAAATCTACAAACCT+GGG - chr1_3:63236725-63236744 Msa0132250:CDS 35.0%
CTAAGGTATGGAAAGAAAGA+AGG + chr1_3:63236753-63236772 None:intergenic 35.0%
!!! GATTTTTGGTCAACGAGTTT+TGG - chr1_3:63237207-63237226 Msa0132250:CDS 35.0%
GCCCACAAATTAATTCCATT+TGG - chr1_3:63237267-63237286 Msa0132250:intron 35.0%
GGTCAGATTTAACTGTTTCA+TGG - chr1_3:63237304-63237323 Msa0132250:intron 35.0%
GAAACAGTGCAAAAACATGT+AGG - chr1_3:63238223-63238242 Msa0132250:intron 35.0%
TGTAGGATATTAAGCTACAC+TGG - chr1_3:63238240-63238259 Msa0132250:intron 35.0%
! TTAAGCTACACTGGTTATCT+TGG - chr1_3:63238249-63238268 Msa0132250:intron 35.0%
TAAACGTGAACGAGTTGTTA+AGG - chr1_3:63238417-63238436 Msa0132250:intron 35.0%
TTTGGATTGAGAGTTTAGGA+GGG + chr1_3:63238952-63238971 None:intergenic 35.0%
TGTGTTTGGATTGAGAGTTT+AGG + chr1_3:63238956-63238975 None:intergenic 35.0%
! AAGTTGATTAAATGAGGGGT+AGG - chr1_3:63239028-63239047 Msa0132250:intron 35.0%
ATCGTGTCAATCTGAAAACT+TGG - chr1_3:63239322-63239341 Msa0132250:intron 35.0%
TCGTGTCAATCTGAAAACTT+GGG - chr1_3:63239323-63239342 Msa0132250:intron 35.0%
TCAATCTGAAAACTTGGGTT+AGG - chr1_3:63239328-63239347 Msa0132250:intron 35.0%
!! TGTCATAAGGGCATTGTTTA+AGG - chr1_3:63239496-63239515 Msa0132250:intron 35.0%
CTACATGGTTAACCTTTGTT+TGG + chr1_3:63239917-63239936 None:intergenic 35.0%
! GAATGATGATGTGTGTACAT+AGG - chr1_3:63239966-63239985 Msa0132250:intron 35.0%
AGCATTGCTTGAGTTATTAG+TGG - chr1_3:63240017-63240036 Msa0132250:intron 35.0%
CACATAGAATGTTCCAAATG+TGG - chr1_3:63240083-63240102 Msa0132250:intron 35.0%
!! AGAGCTACTTTGGAGTTTAT+TGG + chr1_3:63240139-63240158 None:intergenic 35.0%
GTAGAATGAAAGAGCTACTT+TGG + chr1_3:63240149-63240168 None:intergenic 35.0%
CTAATCCCCCTTAATGAAAA+AGG - chr1_3:63240227-63240246 Msa0132250:intron 35.0%
CAATGGAGTAGAAACTCTTA+TGG - chr1_3:63240370-63240389 Msa0132250:intron 35.0%
CTATCAATTGGTCATATCTC+AGG - chr1_3:63240434-63240453 Msa0132250:intron 35.0%
TTTGTATATGCAATGCAGGT+TGG - chr1_3:63240907-63240926 Msa0132250:intron 35.0%
TTGCTCAACATTCTTATCTC+AGG - chr1_3:63240964-63240983 Msa0132250:intron 35.0%
TGCTCAACATTCTTATCTCA+GGG - chr1_3:63240965-63240984 Msa0132250:intron 35.0%
AACATTCTTATCTCAGGGTA+TGG - chr1_3:63240970-63240989 Msa0132250:intron 35.0%
ATATTCGGAGACTAACTTGA+TGG + chr1_3:63241151-63241170 None:intergenic 35.0%
ATGTTGTGGTTGTAAATGCT+AGG - chr1_3:63241319-63241338 Msa0132250:five_prime_UTR 35.0%
CAAGCATCTCTGGATATATT+TGG - chr1_3:63241407-63241426 Msa0132250:five_prime_UTR 35.0%
GTTATCACGAAGCTTGATAA+GGG + chr1_3:63241475-63241494 None:intergenic 35.0%
CTTATCAAGCTTCGTGATAA+CGG - chr1_3:63241474-63241493 Msa0132250:five_prime_UTR 35.0%
AGCTTGAAATATGAGAAGTG+GGG + chr1_3:63241567-63241586 None:intergenic 35.0%
GAGCTTGAAATATGAGAAGT+GGG + chr1_3:63241568-63241587 None:intergenic 35.0%
AGAGCTTGAAATATGAGAAG+TGG + chr1_3:63241569-63241588 None:intergenic 35.0%
!! TGTGTCATGAAAGCTTTGTT+TGG - chr1_3:63241696-63241715 Msa0132250:five_prime_UTR 35.0%
!! TAATGGCAAGATTTTAGCCA+TGG - chr1_3:63241734-63241753 Msa0132250:five_prime_UTR 35.0%
! GCCATGGTTTGTATTATGAA+TGG - chr1_3:63241750-63241769 Msa0132250:five_prime_UTR 35.0%
TCAACAACTTATGGGGATGA+TGG + chr1_3:63236493-63236512 None:intergenic 40.0%
CAAAAAAACGTGGTTGAAGG+TGG + chr1_3:63236548-63236567 None:intergenic 40.0%
AGGCAAAAAAACGTGGTTGA+AGG + chr1_3:63236551-63236570 None:intergenic 40.0%
TCAACGTGAGTCATTTGCAA+TGG - chr1_3:63237067-63237086 Msa0132250:intron 40.0%
CAACGTGAGTCATTTGCAAT+GGG - chr1_3:63237068-63237087 Msa0132250:intron 40.0%
AACGTGAGTCATTTGCAATG+GGG - chr1_3:63237069-63237088 Msa0132250:intron 40.0%
!! CAACTATTGCTTTTACCACC+CGG + chr1_3:63237113-63237132 None:intergenic 40.0%
! TTCTGAGAAAGAGAGACACT+TGG + chr1_3:63237167-63237186 None:intergenic 40.0%
AAGTGTCTCTCTTTCTCAGA+AGG - chr1_3:63237166-63237185 Msa0132250:intron 40.0%
CATGCAAGCTAGAAACCAAA+TGG + chr1_3:63237285-63237304 None:intergenic 40.0%
!! CATTTGGTTTCTAGCTTGCA+TGG - chr1_3:63237283-63237302 Msa0132250:intron 40.0%
TGTGATGAGATGTGCATGAT+TGG - chr1_3:63237531-63237550 Msa0132250:CDS 40.0%
GTGATGAGATGTGCATGATT+GGG - chr1_3:63237532-63237551 Msa0132250:CDS 40.0%
! GGGAGTACGAGTTAAGTAAA+TGG + chr1_3:63238508-63238527 None:intergenic 40.0%
TGCTTATAATAGTGACCGGA+GGG + chr1_3:63238644-63238663 None:intergenic 40.0%
TTGCTTATAATAGTGACCGG+AGG + chr1_3:63238643-63238662 None:intergenic 40.0%
TTGCTTATAATAGTGACCGG+AGG - chr1_3:63238643-63238662 Msa0132250:intron 40.0%
!! TTTTACTAAGGGTGTGTGGA+TGG - chr1_3:63238728-63238747 Msa0132250:intron 40.0%
ATGGAGAGTTTAAGAGAGGA+TGG - chr1_3:63238747-63238766 Msa0132250:intron 40.0%
TGGAGAGTTTAAGAGAGGAT+GGG - chr1_3:63238748-63238767 Msa0132250:intron 40.0%
AGTTTAGGAGGGAAAAGGAA+GGG + chr1_3:63238941-63238960 None:intergenic 40.0%
TTGAGAGTTTAGGAGGGAAA+AGG + chr1_3:63238946-63238965 None:intergenic 40.0%
GTTTGGATTGAGAGTTTAGG+AGG + chr1_3:63238953-63238972 None:intergenic 40.0%
CTAATATATGCAGCAACAGC+AGG - chr1_3:63240326-63240345 Msa0132250:intron 40.0%
AATGAATTGAACGTACCGCA+CGG + chr1_3:63240712-63240731 None:intergenic 40.0%
!! TTTCGCGGCAACTTTTTAAG+TGG + chr1_3:63240791-63240810 None:intergenic 40.0%
GTCTGAATAATGACAGGATC+AGG - chr1_3:63241178-63241197 Msa0132250:CDS 40.0%
CTTGTAATTTCCTGCTGCAA+CGG + chr1_3:63241391-63241410 None:intergenic 40.0%
CAGGAAATTACAAGCATCTC+TGG - chr1_3:63241397-63241416 Msa0132250:five_prime_UTR 40.0%
GCATCTCTGGATATATTTGG+TGG - chr1_3:63241410-63241429 Msa0132250:five_prime_UTR 40.0%
CGTTATCACGAAGCTTGATA+AGG + chr1_3:63241476-63241495 None:intergenic 40.0%
GCTTGAAATATGAGAAGTGG+GGG + chr1_3:63241566-63241585 None:intergenic 40.0%
CTCGATTACACTCGATTACA+AGG + chr1_3:63241635-63241654 None:intergenic 40.0%
! ATGATGGAGACGAAGTGATG+AGG + chr1_3:63236477-63236496 None:intergenic 45.0%
TGGATGAGAGGACTAATGGT+CGG + chr1_3:63236528-63236547 None:intergenic 45.0%
AAGGTGGATGAGAGGACTAA+TGG + chr1_3:63236532-63236551 None:intergenic 45.0%
AAAATGCCAGAATCGGAGTC+AGG - chr1_3:63236960-63236979 Msa0132250:intron 45.0%
AAATGCCAGAATCGGAGTCA+GGG - chr1_3:63236961-63236980 Msa0132250:intron 45.0%
AGATGAGAATAATGGCCCTC+CGG + chr1_3:63237027-63237046 None:intergenic 45.0%
!! TGGAATTAATTTGTGGGCGG+TGG + chr1_3:63237265-63237284 None:intergenic 45.0%
TACTTAACTCGTACTCCCTC+CGG - chr1_3:63238510-63238529 Msa0132250:intron 45.0%
TGGCTGTCATATACTTCCTC+CGG + chr1_3:63238662-63238681 None:intergenic 45.0%
GACAGCCAAAATTTCAGGGA+CGG - chr1_3:63238674-63238693 Msa0132250:intron 45.0%
! CATGTCCGTCCCTGAAATTT+TGG + chr1_3:63238682-63238701 None:intergenic 45.0%
GTGGATGGAGAGTTTAAGAG+AGG - chr1_3:63238743-63238762 Msa0132250:intron 45.0%
AGAGTTTAAGAGAGGATGGG+AGG - chr1_3:63238751-63238770 Msa0132250:intron 45.0%
GAGTTTAAGAGAGGATGGGA+GGG - chr1_3:63238752-63238771 Msa0132250:intron 45.0%
AGTTTAAGAGAGGATGGGAG+GGG - chr1_3:63238753-63238772 Msa0132250:intron 45.0%
GTTTAGGAGGGAAAAGGAAG+GGG + chr1_3:63238940-63238959 None:intergenic 45.0%
GAGTTTAGGAGGGAAAAGGA+AGG + chr1_3:63238942-63238961 None:intergenic 45.0%
ACAGATTGGAGCAGAGTTTG+TGG - chr1_3:63240292-63240311 Msa0132250:intron 45.0%
CAGATTGGAGCAGAGTTTGT+GGG - chr1_3:63240293-63240312 Msa0132250:intron 45.0%
AGAGACTTGTGCTGCAATGT+AGG + chr1_3:63240531-63240550 None:intergenic 45.0%
GTTTACCATCCTTTCCTCTC+TGG - chr1_3:63240584-63240603 Msa0132250:intron 45.0%
CTTGTCCAACGCTAACAGTA+GGG + chr1_3:63240622-63240641 None:intergenic 45.0%
ATGAATTGAACGTACCGCAC+GGG + chr1_3:63240711-63240730 None:intergenic 45.0%
!! TGCAATGCAGGTTGGTGAAT+TGG - chr1_3:63240915-63240934 Msa0132250:intron 45.0%
GAATTGGCTGGTATTGCTGT+TGG - chr1_3:63240931-63240950 Msa0132250:intron 45.0%
GTAAATGCTAGGCCAACAAG+CGG - chr1_3:63241330-63241349 Msa0132250:five_prime_UTR 45.0%
!! AAAATTAAAAAAAAAAAGTT+AGG - chr1_3:63239465-63239484 Msa0132250:intron 5.0%
!!! TTTATTTTACATTTATTTTT+AGG + chr1_3:63239618-63239637 None:intergenic 5.0%
TGGAAAGAAAGAAGGGACCC+AGG + chr1_3:63236745-63236764 None:intergenic 50.0%
GATGAGAATAATGGCCCTCC+GGG + chr1_3:63237026-63237045 None:intergenic 50.0%
GTGAGTCATTTGCAATGGGG+AGG - chr1_3:63237072-63237091 Msa0132250:intron 50.0%
GAGGTGCAACATGTGTATGC+CGG - chr1_3:63237091-63237110 Msa0132250:intron 50.0%
AGGTGCAACATGTGTATGCC+GGG - chr1_3:63237092-63237111 Msa0132250:intron 50.0%
AGAATGTTAACCAGTGCCCC+CGG - chr1_3:63239141-63239160 Msa0132250:intron 50.0%
! TTGGTGCTTAACCAATGCCC+CGG + chr1_3:63239163-63239182 None:intergenic 50.0%
GAATTCCTTAACCAGTGCCC+CGG - chr1_3:63239270-63239289 Msa0132250:intron 50.0%
AATTCCTTAACCAGTGCCCC+GGG - chr1_3:63239271-63239290 Msa0132250:intron 50.0%
ATGGGAAATGCAGCTTGTGC+TGG - chr1_3:63240389-63240408 Msa0132250:intron 50.0%
ATCACTCACCATTGCCTTCG+CGG - chr1_3:63240472-63240491 Msa0132250:intron 50.0%
ACTTGTGCTGCAATGTAGGC+TGG + chr1_3:63240527-63240546 None:intergenic 50.0%
!! TGCGCTTGTTATGCGCTCAA+AGG - chr1_3:63240560-63240579 Msa0132250:intron 50.0%
TACCATCCTTTCCTCTCTGG+TGG - chr1_3:63240587-63240606 Msa0132250:intron 50.0%
ACTGTCCCTACTGTTAGCGT+TGG - chr1_3:63240614-63240633 Msa0132250:intron 50.0%
GCTTGTCCAACGCTAACAGT+AGG + chr1_3:63240623-63240642 None:intergenic 50.0%
!! TGTTGCTACTGATTCCCGTG+CGG - chr1_3:63240694-63240713 Msa0132250:intron 50.0%
!! ATGCAGGTTGGTGAATTGGC+TGG - chr1_3:63240919-63240938 Msa0132250:intron 50.0%
TTCATGGAACCACCGCTTGT+TGG + chr1_3:63241345-63241364 None:intergenic 50.0%
AAGCGGTGGTTCCATGAATC+CGG - chr1_3:63241347-63241366 Msa0132250:five_prime_UTR 50.0%
TAATTTCCTGCTGCAACGGC+TGG + chr1_3:63241387-63241406 None:intergenic 50.0%
TAGCGACGAAAGCTCCTAAC+CGG + chr1_3:63241516-63241535 None:intergenic 50.0%
CGTGGTTGAAGGTGGATGAG+AGG + chr1_3:63236540-63236559 None:intergenic 55.0%
TGCAACATGTGTATGCCGGG+TGG - chr1_3:63237095-63237114 Msa0132250:intron 55.0%
GAATGTTAACCAGTGCCCCC+GGG - chr1_3:63239142-63239161 Msa0132250:intron 55.0%
AATGTTAACCAGTGCCCCCG+GGG - chr1_3:63239143-63239162 Msa0132250:intron 55.0%
TGGTGCTTAACCAATGCCCC+GGG + chr1_3:63239162-63239181 None:intergenic 55.0%
ATTCCTTAACCAGTGCCCCG+GGG - chr1_3:63239272-63239291 Msa0132250:intron 55.0%
GCGAAGGCAATGGTGAGTGA+TGG + chr1_3:63240473-63240492 None:intergenic 55.0%
GAACATGTGCCCACGGGAAA+TGG + chr1_3:63240505-63240524 None:intergenic 55.0%
TAGGCTGGAACATGTGCCCA+CGG + chr1_3:63240512-63240531 None:intergenic 55.0%
CACCACCAGAGAGGAAAGGA+TGG + chr1_3:63240592-63240611 None:intergenic 55.0%
GTGACACCACCAGAGAGGAA+AGG + chr1_3:63240596-63240615 None:intergenic 55.0%
AATGCTAGGCCAACAAGCGG+TGG - chr1_3:63241333-63241352 Msa0132250:five_prime_UTR 55.0%
TAAGGTGCGCACCGGATTCA+TGG + chr1_3:63241361-63241380 None:intergenic 55.0%
ATGAATCCGGTGCGCACCTT+AGG - chr1_3:63241360-63241379 Msa0132250:five_prime_UTR 55.0%
!! TATTTGGTGGCACCGACGCT+TGG - chr1_3:63241423-63241442 Msa0132250:five_prime_UTR 55.0%
!! CTTGGTGCTCTAGCTGGTTC+TGG - chr1_3:63241441-63241460 Msa0132250:five_prime_UTR 55.0%
CTGAACCACCTCAACCGGTT+AGG - chr1_3:63241499-63241518 Msa0132250:five_prime_UTR 55.0%
AAGCTCCTAACCGGTTGAGG+TGG + chr1_3:63241507-63241526 None:intergenic 55.0%
CGAAAGCTCCTAACCGGTTG+AGG + chr1_3:63241510-63241529 None:intergenic 55.0%
GGTGTCCCTGACTCCGATTC+TGG + chr1_3:63236969-63236988 None:intergenic 60.0%
ATCGGAGTCAGGGACACCGA+CGG - chr1_3:63236971-63236990 Msa0132250:intron 60.0%
!! AATGGCCCTCCGGGAGTATC+CGG + chr1_3:63237017-63237036 None:intergenic 60.0%
GTGCTTAACCAATGCCCCGG+GGG + chr1_3:63239160-63239179 None:intergenic 60.0%
GGTGCTTAACCAATGCCCCG+GGG + chr1_3:63239161-63239180 None:intergenic 60.0%
TTCCTTAACCAGTGCCCCGG+GGG - chr1_3:63239273-63239292 Msa0132250:intron 60.0%
TCTTGTTAACCGGTGCCCCC+GGG + chr1_3:63239291-63239310 None:intergenic 60.0%
! GTCTTGTTAACCGGTGCCCC+CGG + chr1_3:63239292-63239311 None:intergenic 60.0%
GAAATGGCGAAACGCCGCGA+AGG + chr1_3:63240489-63240508 None:intergenic 60.0%
GGCGTTTCGCCATTTCCCGT+GGG - chr1_3:63240493-63240512 Msa0132250:intron 60.0%
AGGCTGGAACATGTGCCCAC+GGG + chr1_3:63240511-63240530 None:intergenic 60.0%
GGACAGTGACACCACCAGAG+AGG + chr1_3:63240601-63240620 None:intergenic 60.0%
TGCTGCAACGGCTGGACCTA+AGG + chr1_3:63241379-63241398 None:intergenic 60.0%
TTAGGTCCAGCCGTTGCAGC+AGG - chr1_3:63241378-63241397 Msa0132250:five_prime_UTR 60.0%
! CCAGCTAGAGCACCAAGCGT+CGG + chr1_3:63241438-63241457 None:intergenic 60.0%
CGGAGCTGAACCACCTCAAC+CGG - chr1_3:63241494-63241513 Msa0132250:five_prime_UTR 60.0%
GGAGCTTTCGTCGCTAGAGC+TGG - chr1_3:63241520-63241539 Msa0132250:five_prime_UTR 60.0%
!! AAGTGGGGGTGTCGAGAGCA+GGG + chr1_3:63241552-63241571 None:intergenic 60.0%
CGACACCGGATACTCCCGGA+GGG - chr1_3:63237009-63237028 Msa0132250:intron 65.0%
CTTGTTAACCGGTGCCCCCG+GGG + chr1_3:63239290-63239309 None:intergenic 65.0%
GCGAAACGCCGCGAAGGCAA+TGG + chr1_3:63240483-63240502 None:intergenic 65.0%
CGGCGTTTCGCCATTTCCCG+TGG - chr1_3:63240492-63240511 Msa0132250:intron 65.0%
GCTGGACCTAAGGTGCGCAC+CGG + chr1_3:63241369-63241388 None:intergenic 65.0%
!! CCGACGCTTGGTGCTCTAGC+TGG - chr1_3:63241435-63241454 Msa0132250:five_prime_UTR 65.0%
!! GAAGTGGGGGTGTCGAGAGC+AGG + chr1_3:63241553-63241572 None:intergenic 65.0%
AGGGACACCGACGGCAGCGT+CGG - chr1_3:63236980-63236999 Msa0132250:intron 70.0%
!! CCGGGAGTATCCGGTGTCGC+CGG + chr1_3:63237008-63237027 None:intergenic 70.0%
CCGGCGACACCGGATACTCC+CGG - chr1_3:63237005-63237024 Msa0132250:intron 70.0%
GCGACACCGGATACTCCCGG+AGG - chr1_3:63237008-63237027 Msa0132250:intron 70.0%
AACCAGTGCCCCCGGGGCAT+TGG - chr1_3:63239149-63239168 Msa0132250:intron 70.0%
! AACCAATGCCCCGGGGGCAC+TGG + chr1_3:63239154-63239173 None:intergenic 70.0%
!! TGCCCCCGGGGCACTGGTTA+AGG + chr1_3:63239278-63239297 None:intergenic 70.0%
!! ACCGACGGCAGCGTCGGTGC+CGG - chr1_3:63236986-63237005 Msa0132250:intron 75.0%
!! AGCGTCGGTGCCGGCGACAC+CGG - chr1_3:63236995-63237014 Msa0132250:intron 75.0%
! AACCAGTGCCCCGGGGGCAC+CGG - chr1_3:63239279-63239298 Msa0132250:intron 75.0%
!!! GCCGGCACCGACGCTGCCGT+CGG + chr1_3:63236990-63237009 None:intergenic 80.0%
! AACCGGTGCCCCCGGGGCAC+TGG + chr1_3:63239284-63239303 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 63236374 63241808 63236374 ID=Msa0132250;Name=Msa0132250
chr1_3 mRNA 63236374 63241808 63236374 ID=Msa0132250-mRNA-1;Parent=Msa0132250;Name=Msa0132250-mRNA-1;_AED=0.03;_eAED=0.03;_QI=589|1|1|1|1|1|4|272|300
chr1_3 exon 63236374 63236841 63236374 ID=Msa0132250-mRNA-1:exon:21179;Parent=Msa0132250-mRNA-1
chr1_3 exon 63237196 63237257 63237196 ID=Msa0132250-mRNA-1:exon:21178;Parent=Msa0132250-mRNA-1
chr1_3 exon 63237467 63237886 63237467 ID=Msa0132250-mRNA-1:exon:21177;Parent=Msa0132250-mRNA-1
chr1_3 exon 63240995 63241808 63240995 ID=Msa0132250-mRNA-1:exon:21176;Parent=Msa0132250-mRNA-1
chr1_3 five_prime_UTR 63241220 63241808 63241220 ID=Msa0132250-mRNA-1:five_prime_utr;Parent=Msa0132250-mRNA-1
chr1_3 CDS 63240995 63241219 63240995 ID=Msa0132250-mRNA-1:cds;Parent=Msa0132250-mRNA-1
chr1_3 CDS 63237467 63237886 63237467 ID=Msa0132250-mRNA-1:cds;Parent=Msa0132250-mRNA-1
chr1_3 CDS 63237196 63237257 63237196 ID=Msa0132250-mRNA-1:cds;Parent=Msa0132250-mRNA-1
chr1_3 CDS 63236646 63236841 63236646 ID=Msa0132250-mRNA-1:cds;Parent=Msa0132250-mRNA-1
chr1_3 three_prime_UTR 63236374 63236645 63236374 ID=Msa0132250-mRNA-1:three_prime_utr;Parent=Msa0132250-mRNA-1
Gene Sequence

>Msa0132250

ATGCCAGAATCGGAGTCAGGGACACCGACGGCAGCGTCGGTGCCGGCGACACCGGATACTCCCGGAGGGCCATTATTCTCATCTATAAGAATTGATTCACTTGATCAACGTGAGTCATTTGCAATGGGGAGGTGCAACATGTGTATGCCGGGTGGTAAAAGCAATAGTTGCATCGCTAATTTCTCTGCTGCAGTTCGTATTCCAAGTGTCTCTCTTTCTCAGAAGATTGGAGCAGAGTTTGTGGGAACATTCATACTAATATATGCAGCAACAGCAGGACCAATAGTGAACAACAAATACAATGGAGTAGAAACTCTTATGGGAAATGCAGCTTGTGCTGGACTAACAGTCATGTTCATTATTCTATCAATTGGTCATATCTCAGGTGCACATCTCAATCCATCACTCACCATTGCCTTCGCGGCGTTTCGCCATTTCCCGTGGGCACATGTTCCAGCCTACATTGCAGCACAAGTCTCTGCTTCGATTTGCGCTTGTTATGCGCTCAAAGGAGTTTACCATCCTTTCCTCTCTGGTGGTGTCACTGTCCCTACTGTTAGCGTTGGACAAGCTTTTGCAACTGAGTTTATCATCACTTTTATTCTCTTGTTTGTTGTTACTGCTGTTGCTACTGATTCCCGTGCGGTTGGTGAATTGGCTGGTATTGCTGTTGGTGCTACAGTTTTGCTCAACATTCTTATCTCAGGGCCAACAAGCGGTGGTTCCATGAATCCGGTGCGCACCTTAGGTCCAGCCGTTGCAGCAGGAAATTACAAGCATCTCTGGATATATTTGGTGGCACCGACGCTTGGTGCTCTAGCTGGTTCTGGAGTTTATACCCTTATCAAGCTTCGTGATAACGGAGCTGAACCACCTCAACCGGTTAGGAGCTTTCGTCGCTAG
Protein sequence

>Msa0132250

MPESESGTPTAASVPATPDTPGGPLFSSIRIDSLDQRESFAMGRCNMCMPGGKSNSCIANFSAAVRIPSVSLSQKIGAEFVGTFILIYAATAGPIVNNKYNGVETLMGNAACAGLTVMFIILSIGHISGAHLNPSLTIAFAAFRHFPWAHVPAYIAAQVSASICACYALKGVYHPFLSGGVTVPTVSVGQAFATEFIITFILLFVVTAVATDSRAVGELAGIAVGATVLLNILISGPTSGGSMNPVRTLGPAVAAGNYKHLWIYLVAPTLGALAGSGVYTLIKLRDNGAEPPQPVRSFRR*