AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0639310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0639310 MtrunA17_Chr4g0050781 97.653 213 1 2 1 211 1 211 1.31e-138 386
Msa0639310 MtrunA17_Chr1g0210241 43.382 136 73 3 1 135 1 133 2.41e-31 115
Msa0639310 MtrunA17_Chr4g0035031 59.524 84 34 0 2 85 3 86 7.47e-30 109
Msa0639310 MtrunA17_Chr4g0035001 58.228 79 33 0 6 84 5 83 9.44e-29 105
Msa0639310 MtrunA17_Chr4g0035051 55.696 79 35 0 6 84 7 85 7.61e-27 99.4
Msa0639310 MtrunA17_Chr7g0266751 43.038 79 45 0 7 85 32 110 9.95e-19 79.0
Msa0639310 MtrunA17_Chr3g0086861 40.476 84 50 0 1 84 38 121 4.54e-18 81.6
Msa0639310 MtrunA17_Chr3g0119311 47.368 76 40 0 8 83 37 112 2.58e-17 75.1
Msa0639310 MtrunA17_Chr3g0116631 45.455 88 46 1 4 89 203 290 6.98e-17 77.0
Msa0639310 MtrunA17_Chr1g0168601 41.975 81 47 0 4 84 150 230 8.41e-17 76.3
Msa0639310 MtrunA17_Chr7g0263001 46.250 80 43 0 8 87 36 115 1.15e-16 73.6
Msa0639310 MtrunA17_Chr1g0198431 44.156 77 43 0 8 84 193 269 1.84e-16 75.9
Msa0639310 MtrunA17_Chr4g0061451 36.905 84 53 0 1 84 36 119 2.97e-16 75.1
Msa0639310 MtrunA17_Chr7g0219021 40.789 76 45 0 8 83 41 116 3.56e-16 74.7
Msa0639310 MtrunA17_Chr3g0121631 41.975 81 46 1 8 88 37 116 1.84e-15 70.5
Msa0639310 MtrunA17_Chr4g0017401 46.479 71 37 1 3 72 12 82 3.65e-15 72.0
Msa0639310 MtrunA17_Chr2g0327921 40.244 82 49 0 8 89 8 89 4.68e-15 67.8
Msa0639310 MtrunA17_Chr7g0276531 45.833 72 35 1 3 70 14 85 1.16e-14 70.5
Msa0639310 MtrunA17_Chr6g0481071 41.026 78 46 0 8 85 96 173 1.49e-14 68.9
Msa0639310 MtrunA17_Chr8g0346011 40.741 81 41 2 2 76 26 105 3.14e-14 69.7
Msa0639310 MtrunA17_Chr1g0181111 46.053 76 41 0 8 83 197 272 5.90e-14 68.9
Msa0639310 MtrunA17_Chr4g0058151 43.590 78 44 0 7 84 216 293 1.11e-13 68.6
Msa0639310 MtrunA17_Chr4g0058151 38.272 81 50 0 5 85 120 200 5.18e-13 66.6
Msa0639310 MtrunA17_Chr3g0088511 32.353 102 64 1 8 109 33 129 1.16e-13 65.5
Msa0639310 MtrunA17_Chr2g0290191 35.556 90 51 2 8 97 5 87 1.39e-13 68.9
Msa0639310 MtrunA17_Chr2g0290191 36.709 79 49 1 8 86 107 184 6.41e-13 67.0
Msa0639310 MtrunA17_Chr5g0447761 31.250 112 71 3 8 116 7 115 1.74e-13 68.6
Msa0639310 MtrunA17_Chr5g0447761 36.585 82 51 1 8 89 107 187 1.13e-12 66.2
Msa0639310 MtrunA17_Chr1g0186351 39.241 79 47 1 8 86 113 190 2.13e-13 68.2
Msa0639310 MtrunA17_Chr1g0186351 33.333 81 52 1 3 83 2 80 6.14e-12 63.9
Msa0639310 MtrunA17_Chr6g0449481 31.325 83 57 0 8 90 134 216 2.19e-13 68.2
Msa0639310 MtrunA17_Chr3g0101061 31.624 117 74 3 3 116 36 149 4.22e-13 67.4
Msa0639310 MtrunA17_Chr3g0101061 35.366 82 52 1 8 89 141 221 4.05e-11 61.6
Msa0639310 MtrunA17_Chr3g0144311 48.276 58 30 0 3 60 25 82 6.85e-13 65.9
Msa0639310 MtrunA17_Chr3g0120721 35.000 80 52 0 8 87 107 186 7.32e-13 66.2
Msa0639310 MtrunA17_Chr4g0042161 42.353 85 43 2 4 85 48 129 9.58e-13 63.2
Msa0639310 MtrunA17_Chr7g0267571 37.662 77 48 0 7 83 212 288 1.14e-12 65.5
Msa0639310 MtrunA17_Chr7g0268551 39.189 74 45 0 9 82 350 423 1.36e-12 65.9
Msa0639310 MtrunA17_Chr5g0426311 42.029 69 39 1 8 76 17 84 1.92e-12 64.7
Msa0639310 MtrunA17_Chr3g0135111 36.364 88 53 2 8 94 118 203 3.02e-12 64.7
Msa0639310 MtrunA17_Chr4g0065741 36.471 85 52 1 8 92 72 154 5.54e-12 61.6
Msa0639310 MtrunA17_Chr4g0031091 38.356 73 45 0 8 80 34 106 6.69e-12 60.1
Msa0639310 MtrunA17_Chr3g0080801 28.030 132 79 3 9 140 70 185 7.22e-12 63.2
Msa0639310 MtrunA17_Chr5g0419351 30.645 124 73 3 9 122 12 132 1.11e-11 60.8
Msa0639310 MtrunA17_Chr3g0110211 44.737 76 41 1 9 83 114 189 1.24e-11 62.8
Msa0639310 MtrunA17_Chr8g0371201 40.789 76 45 0 9 84 64 139 2.24e-11 61.6
Msa0639310 MtrunA17_Chr1g0177101 33.333 90 60 0 7 96 207 296 3.65e-11 61.2
Msa0639310 MtrunA17_Chr2g0281891 32.222 90 61 0 8 97 299 388 6.22e-11 61.2
Msa0639310 MtrunA17_Chr6g0484281 27.778 144 85 4 9 152 245 369 9.05e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0639310 AT3G26420.1 53.755 253 62 7 1 208 1 243 9.42e-69 211
Msa0639310 AT4G39260.3 60.976 82 32 0 4 85 3 84 1.50e-30 108
Msa0639310 AT4G39260.1 61.728 81 31 0 4 84 3 83 4.55e-29 107
Msa0639310 AT2G21660.2 60.759 79 31 0 6 84 7 85 1.13e-28 105
Msa0639310 AT1G60650.2 41.221 131 68 3 6 136 11 132 1.74e-28 108
Msa0639310 AT1G60650.1 41.221 131 68 3 6 136 11 132 1.74e-28 108
Msa0639310 AT2G21660.1 60.759 79 31 0 6 84 7 85 1.78e-28 105
Msa0639310 AT5G04280.1 38.953 172 87 4 1 159 1 167 8.87e-26 102
Msa0639310 AT4G39260.4 60.606 66 26 0 4 69 3 68 2.40e-22 87.8
Msa0639310 AT4G13850.3 46.429 84 45 0 8 91 36 119 7.54e-19 79.7
Msa0639310 AT4G13850.2 46.429 84 45 0 8 91 36 119 9.37e-19 79.7
Msa0639310 AT4G13850.4 49.351 77 39 0 8 84 36 112 1.00e-18 79.0
Msa0639310 AT4G13850.1 46.429 84 45 0 8 91 36 119 1.05e-18 79.7
Msa0639310 AT3G23830.2 43.023 86 49 0 8 93 36 121 5.24e-18 77.4
Msa0639310 AT3G23830.1 43.023 86 49 0 8 93 36 121 5.24e-18 77.4
Msa0639310 AT2G37220.1 48.052 77 40 0 8 84 205 281 1.01e-17 79.7
Msa0639310 AT2G37220.1 38.202 89 53 1 6 94 90 176 3.53e-12 64.3
Msa0639310 AT3G53460.1 46.753 77 41 0 8 84 258 334 6.19e-17 78.2
Msa0639310 AT3G53460.3 46.753 77 41 0 8 84 258 334 6.19e-17 78.2
Msa0639310 AT3G53460.2 46.753 77 41 0 8 84 258 334 6.19e-17 78.2
Msa0639310 AT5G06210.1 42.500 80 46 0 8 87 35 114 6.48e-17 74.7
Msa0639310 AT5G61030.2 43.421 76 43 0 8 83 41 116 7.77e-17 77.8
Msa0639310 AT5G61030.1 43.421 76 43 0 8 83 41 116 7.77e-17 77.8
Msa0639310 AT3G53460.4 46.753 77 41 0 8 84 279 355 1.02e-16 77.8
Msa0639310 AT1G18630.1 50.000 76 38 0 8 83 37 112 1.21e-16 74.3
Msa0639310 AT1G33470.3 47.945 73 37 1 1 72 1 73 4.93e-16 74.3
Msa0639310 AT1G33470.1 47.945 73 37 1 1 72 1 73 6.09e-16 74.3
Msa0639310 AT1G33470.2 47.945 73 37 1 1 72 1 73 6.12e-16 74.3
Msa0639310 AT2G46780.1 46.667 75 39 1 2 76 17 90 1.88e-15 73.9
Msa0639310 AT2G46780.2 46.667 75 39 1 2 76 17 90 1.88e-15 73.9
Msa0639310 AT1G22910.1 46.575 73 38 1 1 72 7 79 1.99e-15 73.2
Msa0639310 AT4G24770.1 43.210 81 46 0 4 84 241 321 2.12e-15 73.9
Msa0639310 AT4G24770.1 38.272 81 50 0 5 85 148 228 1.64e-12 65.9
Msa0639310 AT4G24770.2 43.210 81 46 0 4 84 241 321 2.12e-15 73.9
Msa0639310 AT4G24770.2 38.272 81 50 0 5 85 148 228 1.64e-12 65.9
Msa0639310 AT5G04280.3 40.299 134 62 4 39 159 1 129 2.51e-15 73.2
Msa0639310 AT5G04280.4 40.299 134 62 4 39 159 1 129 2.51e-15 73.2
Msa0639310 AT5G04280.5 40.299 134 62 4 39 159 1 129 2.51e-15 73.2
Msa0639310 AT5G04280.2 40.299 134 62 4 39 159 1 129 2.51e-15 73.2
Msa0639310 AT1G22910.2 46.575 73 38 1 1 72 7 79 2.71e-15 72.4
Msa0639310 AT5G47320.1 35.922 103 64 1 8 108 32 134 3.90e-15 71.6
Msa0639310 AT5G59865.1 43.038 79 45 0 8 86 27 105 4.84e-15 68.9
Msa0639310 AT1G60000.1 41.667 84 49 0 1 84 171 254 5.22e-15 72.0
Msa0639310 AT2G21690.1 46.377 69 37 0 1 69 1 69 5.63e-15 68.9
Msa0639310 AT1G73530.1 35.514 107 61 2 8 111 78 179 5.97e-15 70.5
Msa0639310 AT1G22910.3 46.575 73 38 1 1 72 7 79 2.06e-14 71.2
Msa0639310 AT1G60650.3 36.538 104 57 3 33 136 1 95 2.17e-14 70.1
Msa0639310 AT2G37510.1 50.000 74 37 0 8 81 35 108 2.53e-14 67.8
Msa0639310 AT3G20930.1 41.558 77 45 0 8 84 283 359 3.37e-14 70.9
Msa0639310 AT1G74230.1 43.421 76 42 1 8 83 35 109 6.18e-14 69.3
Msa0639310 AT1G03457.3 41.096 73 43 0 9 81 227 299 7.38e-14 69.3
Msa0639310 AT3G13224.2 32.143 84 57 0 8 91 110 193 8.75e-14 69.3
Msa0639310 AT1G60650.4 38.776 98 51 3 39 136 1 89 1.03e-13 68.2
Msa0639310 AT3G08000.1 44.737 76 42 0 8 83 42 117 1.07e-13 66.2
Msa0639310 AT4G14300.1 37.805 82 49 1 3 84 2 81 1.08e-13 69.3
Msa0639310 AT4G14300.1 36.709 79 49 1 8 86 111 188 4.98e-12 64.7
Msa0639310 AT4G14300.2 37.805 82 49 1 3 84 2 81 1.08e-13 69.3
Msa0639310 AT4G14300.2 36.709 79 49 1 8 86 111 188 4.98e-12 64.7
Msa0639310 AT4G26650.1 32.456 114 70 2 2 115 10 116 1.21e-13 69.3
Msa0639310 AT4G26650.2 32.456 114 70 2 2 115 7 113 1.32e-13 69.3
Msa0639310 AT1G78260.1 43.478 69 38 1 8 76 18 85 1.44e-13 68.2
Msa0639310 AT3G07810.2 34.615 78 49 1 8 85 7 82 1.49e-13 69.3
Msa0639310 AT3G07810.2 37.805 82 50 1 8 89 109 189 1.25e-12 66.6
Msa0639310 AT3G07810.1 34.615 78 49 1 8 85 7 82 1.53e-13 68.9
Msa0639310 AT3G07810.1 37.805 82 50 1 8 89 109 189 1.38e-12 66.2
Msa0639310 AT3G13224.3 32.143 84 57 0 8 91 101 184 1.90e-13 68.6
Msa0639310 AT1G03457.2 41.096 73 43 0 9 81 341 413 2.25e-13 68.6
Msa0639310 AT4G13860.1 42.466 73 42 0 8 80 4 76 2.30e-13 63.5
Msa0639310 AT1G03457.1 41.096 73 43 0 9 81 332 404 2.33e-13 68.6
Msa0639310 AT1G76460.1 50.943 53 26 0 8 60 25 77 2.56e-13 67.8
Msa0639310 AT5G47620.1 37.179 78 47 1 8 85 7 82 3.49e-13 68.2
Msa0639310 AT5G47620.2 37.179 78 47 1 8 85 7 82 3.49e-13 68.2
Msa0639310 AT5G47620.5 37.179 78 47 1 8 85 7 82 3.49e-13 68.2
Msa0639310 AT1G22330.1 39.189 74 44 1 3 76 13 85 7.18e-13 66.2
Msa0639310 AT1G78260.2 43.478 69 38 1 8 76 18 85 7.78e-13 66.2
Msa0639310 AT1G20880.2 45.455 66 35 1 8 72 25 90 8.65e-13 65.9
Msa0639310 AT1G20880.3 45.455 66 35 1 8 72 25 90 8.65e-13 65.9
Msa0639310 AT1G20880.1 45.455 66 35 1 8 72 25 90 8.65e-13 65.9
Msa0639310 AT5G55550.5 30.952 126 79 3 8 133 7 124 9.77e-13 66.6
Msa0639310 AT5G55550.4 30.952 126 79 3 8 133 7 124 9.77e-13 66.6
Msa0639310 AT5G55550.9 30.952 126 79 3 8 133 7 124 9.77e-13 66.6
Msa0639310 AT5G55550.7 30.952 126 79 3 8 133 7 124 9.77e-13 66.6
Msa0639310 AT5G55550.1 30.952 126 79 3 8 133 7 124 9.77e-13 66.6
Msa0639310 AT5G55550.2 32.075 106 65 2 8 113 7 105 1.17e-12 66.6
Msa0639310 AT5G55550.3 32.075 106 65 2 8 113 7 105 1.17e-12 66.6
Msa0639310 AT5G55550.6 32.075 106 65 2 8 113 7 105 1.17e-12 66.6
Msa0639310 AT5G55550.11 32.075 106 65 2 8 113 7 105 1.17e-12 66.6
Msa0639310 AT5G40490.1 36.145 83 53 0 8 90 131 213 1.32e-12 66.2
Msa0639310 AT1G58470.2 36.364 88 55 1 8 95 121 207 1.83e-12 65.9
Msa0639310 AT1G58470.1 36.364 88 55 1 8 95 121 207 1.83e-12 65.9
Msa0639310 AT5G50250.1 41.026 78 46 0 7 84 207 284 3.50e-12 64.3
Msa0639310 AT3G20930.2 39.024 82 45 1 8 84 283 364 3.76e-12 64.7
Msa0639310 AT3G54770.3 41.667 72 38 1 3 70 13 84 3.93e-12 63.9
Msa0639310 AT3G54770.1 41.667 72 38 1 3 70 13 84 4.51e-12 63.9
Msa0639310 AT5G47620.4 40.000 65 39 0 8 72 7 71 8.35e-12 63.9
Msa0639310 AT5G40490.2 37.931 87 46 2 8 90 114 196 9.15e-12 63.9
Msa0639310 AT5G53720.1 41.026 78 45 1 6 83 8 84 1.30e-11 59.3
Msa0639310 AT5G54580.1 40.449 89 47 2 4 89 53 138 1.56e-11 60.5
Msa0639310 AT4G03110.1 41.096 73 43 0 9 81 351 423 2.49e-11 62.4
Msa0639310 AT3G46020.1 34.524 84 55 0 1 84 1 84 3.09e-11 58.5
Msa0639310 AT5G55550.8 38.028 71 42 1 8 78 7 75 4.65e-11 62.0
Msa0639310 AT2G33410.1 34.177 79 51 1 8 86 111 188 5.57e-11 61.6
Msa0639310 AT2G33410.1 32.927 82 53 1 3 84 2 81 7.33e-11 61.2
Msa0639310 AT5G55550.10 38.028 71 42 1 8 78 7 75 5.67e-11 61.6

Find 132 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGATTTGGCTATCCTGTTTC+TGG 0.103545 4_3:+34980607 Msa0639310:three_prime_UTR
TTGGCTAAATGTGGTCATTT+TGG 0.169476 4_3:+34980754 Msa0639310:three_prime_UTR
CTATCCTGTTTCTGGATCTT+TGG 0.177002 4_3:+34980615 Msa0639310:three_prime_UTR
TTCGTTCACAATGTTCTTCT+TGG 0.185957 4_3:+34980167 Msa0639310:three_prime_UTR
TATGCGTTATTGTTCTATTT+GGG 0.210106 4_3:+34980998 Msa0639310:three_prime_UTR
TTATGCGTTATTGTTCTATT+TGG 0.210748 4_3:+34980997 Msa0639310:three_prime_UTR
TCTGGACGCTCTCGTGGTTT+TGG 0.216628 4_3:+34979480 Msa0639310:CDS
TATCACATGCGGCAGTTAAA+AGG 0.269290 4_3:-34981041 None:intergenic
GGTGGTAGTCGTGGAGATTC+TGG 0.273094 4_3:+34979888 Msa0639310:CDS
AGGGATACATTTGAAAAGTT+TGG 0.280841 4_3:+34979126 Msa0639310:CDS
TTGGAAGAGTACCGTGAGTT+AGG 0.287051 4_3:-34978375 None:intergenic
TATCCTGTTTCTGGATCTTT+GGG 0.299460 4_3:+34980616 Msa0639310:three_prime_UTR
ACATGTCCAGTTAGCAATAA+AGG 0.301676 4_3:-34980792 None:intergenic
GTTCTTCTGGCGGTCGCAGC+AGG 0.318323 4_3:+34979862 Msa0639310:CDS
GTTCTTCTTGGATTGTTGTT+TGG 0.332366 4_3:+34980179 Msa0639310:three_prime_UTR
GATTCTATTTAGAGAGGAAT+TGG 0.332823 4_3:+34980473 Msa0639310:three_prime_UTR
AGTGGTGGTGGTGGTCACTA+TGG 0.339869 4_3:+34979819 Msa0639310:CDS
TTAAAAGTATAATGCCGATT+TGG 0.340839 4_3:+34980592 Msa0639310:three_prime_UTR
AGAAATGGAGACCGTTCTTC+TGG 0.343709 4_3:+34979849 Msa0639310:CDS
ACGTTCCTATGCTGGTCTTT+TGG 0.352860 4_3:+34980262 Msa0639310:three_prime_UTR
TGGGTCCCTTTATTGCTAAC+TGG 0.353192 4_3:+34980786 Msa0639310:three_prime_UTR
TTTGCAGCTTTGAAATCAAA+TGG 0.363420 4_3:+34980117 Msa0639310:intron
AAGAAGCAAAACTCTACATT+TGG 0.380905 4_3:-34980549 None:intergenic
AGGGAGTGCCCTAGTGGTGA+AGG 0.394081 4_3:+34979744 Msa0639310:CDS
CCATATGAACGACGTGGATC+TGG 0.395444 4_3:+34979948 Msa0639310:CDS
GAACGGTCTCCATTTCTATC+TGG 0.397184 4_3:-34979843 None:intergenic
TATGATTCTGGCACATTATG+TGG 0.404121 4_3:+34980937 Msa0639310:three_prime_UTR
GTGGAAGAGAATGGAAGTTT+AGG 0.404584 4_3:-34981124 None:intergenic
TTCTTGGATTGTTGTTTGGA+TGG 0.418357 4_3:+34980183 Msa0639310:three_prime_UTR
CAACATCCGATAGAAAGTTA+AGG 0.426777 4_3:+34979106 Msa0639310:CDS
GTTCGTGTGCCAGAGCTTGC+AGG 0.428329 4_3:+34981418 Msa0639310:three_prime_UTR
ACTAATTAACGTTCCTATGC+TGG 0.434677 4_3:+34980254 Msa0639310:three_prime_UTR
GTGGTTGTTGACAAATTCTC+TGG 0.436058 4_3:+34979462 Msa0639310:CDS
GAAAGTAGATATGGTGGAAG+TGG 0.439807 4_3:+34979801 Msa0639310:CDS
TATCAAAACACTAGTATCAT+TGG 0.440320 4_3:-34980207 None:intergenic
CCAGATCCACGTCGTTCATA+TGG 0.442836 4_3:-34979948 None:intergenic
TTCGCACTATTTGTCTATCA+TGG 0.443041 4_3:+34981199 Msa0639310:three_prime_UTR
CATGTCCAGTTAGCAATAAA+GGG 0.444130 4_3:-34980791 None:intergenic
TTTCCCAAAGATCCAGAAAC+AGG 0.446640 4_3:-34980619 None:intergenic
CAGAAACAGGATAGCCAAAT+CGG 0.447584 4_3:-34980606 None:intergenic
ATGGTTGAGTTCACTAGCAT+TGG 0.449938 4_3:+34981150 Msa0639310:three_prime_UTR
CTACATTTGGACCTCCATCT+TGG 0.453690 4_3:-34980536 None:intergenic
CACTATGGTCCAGATAGAAA+TGG 0.454742 4_3:+34979834 Msa0639310:CDS
CGAGATTACCGAGGTGGTCG+AGG 0.461040 4_3:+34979672 Msa0639310:CDS
TGGAAGAGTACCGTGAGTTA+GGG 0.468642 4_3:-34978374 None:intergenic
CCGTGAGTTAGGGCGGAACT+TGG 0.469870 4_3:-34978364 None:intergenic
GGTGGTAGAGAAAGTAGATA+TGG 0.473535 4_3:+34979792 Msa0639310:CDS
CTCTATCAACAGTAATAGTT+CGG 0.474868 4_3:-34979575 None:intergenic
CAGTTTGTTGTGCTATAAAA+CGG 0.477865 4_3:+34981081 Msa0639310:three_prime_UTR
ATGAATGGGATAGATTTAGA+TGG 0.485420 4_3:+34979552 Msa0639310:CDS
AGATATGGTGGAAGTGGTGG+TGG 0.486452 4_3:+34979807 Msa0639310:CDS
GTTATTGCAGTTTAAAATGT+CGG 0.488369 4_3:+34979041 Msa0639310:intron
AACATCCGATAGAAAGTTAA+GGG 0.491433 4_3:+34979107 Msa0639310:CDS
CAAGGATCCTCTAGAGATGA+TGG 0.492928 4_3:+34979609 Msa0639310:CDS
TCTGGCGGTCGCAGCAGGGA+TGG 0.500920 4_3:+34979867 Msa0639310:CDS
TTGCAGCTTTGAAATCAAAT+GGG 0.504229 4_3:+34980118 Msa0639310:intron
TCTTCATGGGGAATATATGA+AGG 0.505584 4_3:+34980901 Msa0639310:three_prime_UTR
CGCGATCGCGACCACGCTCT+CGG 0.506416 4_3:-34979641 None:intergenic
TATGGTGGAAGTGGTGGTGG+TGG 0.507336 4_3:+34979810 Msa0639310:CDS
GGTAGAGAAAGTAGATATGG+TGG 0.507553 4_3:+34979795 Msa0639310:CDS
AATAGTGCGAATATTTAGTA+AGG 0.509280 4_3:-34981187 None:intergenic
CATGCATGTCGAGTGCTGCT+TGG 0.509513 4_3:+34980384 Msa0639310:three_prime_UTR
AACCATGTCGTGGAAGAGAA+TGG 0.511194 4_3:-34981133 None:intergenic
ATTAAATACCTCGATGAGAA+AGG 0.513257 4_3:-34979991 None:intergenic
AAATTCTCTGGACGCTCTCG+TGG 0.522134 4_3:+34979474 Msa0639310:CDS
TGTGCCAGAATCATAGAAAA+CGG 0.524140 4_3:-34980929 None:intergenic
TTCCTCTCTAAATAGAATCA+AGG 0.524710 4_3:-34980469 None:intergenic
GATGCTATTGATGCTATGAA+TGG 0.526053 4_3:+34979537 Msa0639310:CDS
TATCGGATGTTGACCAAGCA+AGG 0.526702 4_3:-34979095 None:intergenic
ATGCGTTATTGTTCTATTTG+GGG 0.526965 4_3:+34980999 Msa0639310:three_prime_UTR
TGTATCCCTTAACTTTCTAT+CGG 0.530467 4_3:-34979112 None:intergenic
ATGCTATCAATAATTGCTGC+TGG 0.532558 4_3:+34980339 Msa0639310:three_prime_UTR
TACCTTGATTCTATTTAGAG+AGG 0.532587 4_3:+34980467 Msa0639310:three_prime_UTR
ACGGTAGTCAAACCTGAGCC+TGG 0.533062 4_3:+34981100 Msa0639310:three_prime_UTR
TGCTTCAATTGTGGTAAACC+TGG 0.534286 4_3:+34979711 Msa0639310:CDS
TTCCATTCTCTTCCACGACA+TGG 0.538850 4_3:+34981131 Msa0639310:three_prime_UTR
GGAAGTTTAGGACCAGGCTC+AGG 0.543204 4_3:-34981112 None:intergenic
GGGAGTGCCCTAGTGGTGAA+GGG 0.544084 4_3:+34979745 Msa0639310:CDS
TTCTTCTGGCGGTCGCAGCA+GGG 0.544328 4_3:+34979863 Msa0639310:CDS
TGGCAAGCTTACAGAGGCCA+AGG 0.548700 4_3:+34979146 Msa0639310:CDS
TGCTGCGACCGCCAGAAGAA+CGG 0.550061 4_3:-34979860 None:intergenic
TATCGTGATCGTGATCGTGC+TGG 0.552506 4_3:+34979924 Msa0639310:CDS
GCAGGATTCTTGGCTAAATG+TGG 0.559112 4_3:+34980745 Msa0639310:intron
CTTCATGGGGAATATATGAA+GGG 0.560134 4_3:+34980902 Msa0639310:three_prime_UTR
GGCGGTCGCAGCAGGGATGG+TGG 0.563764 4_3:+34979870 Msa0639310:CDS
ACAAACCAAAAGACCAGCAT+AGG 0.565468 4_3:-34980267 None:intergenic
TGCGTTATTGTTCTATTTGG+GGG 0.565803 4_3:+34981000 Msa0639310:three_prime_UTR
AGCAGGGATGGTGGTAGTCG+TGG 0.574692 4_3:+34979879 Msa0639310:CDS
TTTATGCATGTCGAGTTGCA+TGG 0.575230 4_3:+34980427 Msa0639310:three_prime_UTR
AAAATGATCAAATCTAACAT+TGG 0.578255 4_3:-34981308 None:intergenic
CACTCCCTAGCAAAATGACC+AGG 0.578775 4_3:-34979729 None:intergenic
GGTGGTCGAGGATCTAATGA+TGG 0.580077 4_3:+34979684 Msa0639310:CDS
GGAGTGCCCTAGTGGTGAAG+GGG 0.582980 4_3:+34979746 Msa0639310:CDS
AGTAGATATGGTGGAAGTGG+TGG 0.583787 4_3:+34979804 Msa0639310:CDS
GCAGTTTAAAATGTCGGACG+AGG 0.587416 4_3:+34979047 Msa0639310:intron
AATGGAGACCGTTCTTCTGG+CGG 0.587666 4_3:+34979852 Msa0639310:CDS
CTTTGGGAAAGTAGTCAAGC+GGG 0.590635 4_3:+34980632 Msa0639310:three_prime_UTR
ATGCTATTGATGCTATGAAT+GGG 0.592487 4_3:+34979538 Msa0639310:CDS
CATTAGAACATTGAATTCAA+CGG 0.594184 4_3:-34980307 None:intergenic
TTTGCTAGGGAGTGCCCTAG+TGG 0.594989 4_3:+34979738 Msa0639310:CDS
TTTGTGGCTTTGGATGTTTG+GGG 0.601798 4_3:+34980501 Msa0639310:three_prime_UTR
GAACGACGTGGATCTGGAGG+CGG 0.604365 4_3:+34979954 Msa0639310:CDS
AACGTTAATTAGTCTAACAA+AGG 0.610574 4_3:-34980244 None:intergenic
CAAACCACATTTCTATGTGG+TGG 0.613393 4_3:+34981394 Msa0639310:three_prime_UTR
GAGAATGGAAGTTTAGGACC+AGG 0.613697 4_3:-34981118 None:intergenic
ATATGATGAAAAGAAAGCAA+TGG 0.616878 4_3:+34979512 Msa0639310:CDS
CCAAGTTCCGCCCTAACTCA+CGG 0.631741 4_3:+34978364 Msa0639310:five_prime_UTR
GAGTGCCCTAGTGGTGAAGG+GGG 0.631815 4_3:+34979747 Msa0639310:CDS
GGTGATCGCTACCGAGAGCG+TGG 0.633883 4_3:+34979630 Msa0639310:CDS
TTCTTCTCATTGGAAGAACG+AGG 0.636743 4_3:+34980567 Msa0639310:three_prime_UTR
TCTCTAGAGGATCCTTGTTG+AGG 0.640001 4_3:-34979603 None:intergenic
TTCATGGGGAATATATGAAG+GGG 0.640366 4_3:+34980903 Msa0639310:three_prime_UTR
TATGAACGACGTGGATCTGG+AGG 0.640937 4_3:+34979951 Msa0639310:CDS
GATAGAGCTCAGCCTCAACA+AGG 0.641644 4_3:+34979591 Msa0639310:CDS
GCGATCACCATCATCTCTAG+AGG 0.642537 4_3:-34979616 None:intergenic
CATTAGATCCTCGACCACCT+CGG 0.645981 4_3:-34979680 None:intergenic
AACAAACAAAATATCACATG+CGG 0.646643 4_3:-34981052 None:intergenic
GAACAAACCACATTTCTATG+TGG 0.653632 4_3:+34981391 Msa0639310:three_prime_UTR
AAGAGTACCGTGAGTTAGGG+CGG 0.658136 4_3:-34978371 None:intergenic
TTGATATACCTTTCTCATCG+AGG 0.661732 4_3:+34979983 Msa0639310:exon
GATGGAGGATGCTTCAATTG+TGG 0.663732 4_3:+34979702 Msa0639310:CDS
AAAGTTTGGCAAGCTTACAG+AGG 0.664669 4_3:+34979140 Msa0639310:CDS
CCCCATGAAGAAAACAAGAG+CGG 0.666738 4_3:-34980889 None:intergenic
TCTTTGGGAAAGTAGTCAAG+CGG 0.667376 4_3:+34980631 Msa0639310:three_prime_UTR
GGTCGAGGATCTAATGATGG+AGG 0.667781 4_3:+34979687 Msa0639310:CDS
AACTCCACCACATAGAAATG+TGG 0.667982 4_3:-34981398 None:intergenic
TCATGGGGAATATATGAAGG+GGG 0.691942 4_3:+34980904 Msa0639310:three_prime_UTR
TAGTGAACTCAACCATGTCG+TGG 0.697075 4_3:-34981143 None:intergenic
CGTGATCGAGATTACCGAGG+TGG 0.705905 4_3:+34979666 Msa0639310:CDS
ATAGAATCAAGGTAATCCCG+CGG 0.718822 4_3:-34980458 None:intergenic
GAACGTGATCGAGATTACCG+AGG 0.730869 4_3:+34979663 Msa0639310:CDS
GCTGGACCATATGAACGACG+TGG 0.755617 4_3:+34979942 Msa0639310:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGTTTTTTTTATTGATTT+AGG + chr4_3:34978621-34978640 Msa0639310:intron 10.0%
!!! GTGTATTTTTTATTTTATTT+TGG + chr4_3:34980014-34980033 Msa0639310:intron 10.0%
!! TATGATAGAAAATAACTAAA+GGG - chr4_3:34978863-34978882 None:intergenic 15.0%
!! TTATGATAGAAAATAACTAA+AGG - chr4_3:34978864-34978883 None:intergenic 15.0%
!! TTACTTGATTTACTAATAAA+AGG - chr4_3:34979323-34979342 None:intergenic 15.0%
!! CAATGTAATATGATAAAAAA+TGG + chr4_3:34979360-34979379 Msa0639310:intron 15.0%
!!! TTTCATTTTATTTTGTGAAA+AGG + chr4_3:34979440-34979459 Msa0639310:intron 15.0%
!! TGAAAAAAATACAATTTGAA+AGG - chr4_3:34981273-34981292 None:intergenic 15.0%
!! TTAAAAATCGGATTTAAATC+AGG - chr4_3:34978465-34978484 None:intergenic 20.0%
!! ATAACATAAGAATTAGTACA+TGG - chr4_3:34979026-34979045 None:intergenic 20.0%
!!! CTTTTGTTCATTTTTATTTG+AGG + chr4_3:34979273-34979292 Msa0639310:intron 20.0%
!! TCTGAACATTATATTTGATT+TGG + chr4_3:34979393-34979412 Msa0639310:intron 20.0%
!!! ATTATATTTGATTTGGAGAA+TGG + chr4_3:34979400-34979419 Msa0639310:intron 20.0%
!!! TTTATTTTATTTTGGACGAA+TGG + chr4_3:34980022-34980041 Msa0639310:intron 20.0%
!! AAAATGATCAAATCTAACAT+TGG - chr4_3:34981311-34981330 None:intergenic 20.0%
! AAGAAAAAAGTGGAAGAATT+TGG - chr4_3:34978397-34978416 None:intergenic 25.0%
! ATGAAGAATGAAGAAAAAAG+TGG - chr4_3:34978407-34978426 None:intergenic 25.0%
!! GTTTTGTATAATTCGTGTTT+GGG + chr4_3:34978512-34978531 Msa0639310:intron 25.0%
!! GTAATTGTTGTTATCATTGT+AGG + chr4_3:34978738-34978757 Msa0639310:intron 25.0%
! GTTGAAAATTCAAACTTATG+AGG - chr4_3:34978814-34978833 None:intergenic 25.0%
!! TTCTATTAGCTTATTGTTTG+AGG + chr4_3:34978934-34978953 Msa0639310:intron 25.0%
! GTTATTTGTGTTAATTACCT+TGG + chr4_3:34978956-34978975 Msa0639310:intron 25.0%
!! GTTATTGCAGTTTAAAATGT+CGG + chr4_3:34979041-34979060 Msa0639310:intron 25.0%
!!! AAAGATAAAGTTTGTTGTCT+AGG - chr4_3:34979257-34979276 None:intergenic 25.0%
!!! CATTTTATTTTGTGAAAAGG+TGG + chr4_3:34979443-34979462 Msa0639310:intron 25.0%
! ATATGATGAAAAGAAAGCAA+TGG + chr4_3:34979512-34979531 Msa0639310:CDS 25.0%
! TATCAAAACACTAGTATCAT+TGG - chr4_3:34980210-34980229 None:intergenic 25.0%
!! AACGTTAATTAGTCTAACAA+AGG - chr4_3:34980247-34980266 None:intergenic 25.0%
! CATTAGAACATTGAATTCAA+CGG - chr4_3:34980310-34980329 None:intergenic 25.0%
!! TTAAAAGTATAATGCCGATT+TGG + chr4_3:34980592-34980611 Msa0639310:three_prime_UTR 25.0%
!! TATTGATGTTATTGAGCTTT+TGG + chr4_3:34980723-34980742 Msa0639310:intron 25.0%
! ACAATAACGCATAAAAACAA+TGG - chr4_3:34980991-34981010 None:intergenic 25.0%
! TTATGCGTTATTGTTCTATT+TGG + chr4_3:34980997-34981016 Msa0639310:three_prime_UTR 25.0%
! TATGCGTTATTGTTCTATTT+GGG + chr4_3:34980998-34981017 Msa0639310:three_prime_UTR 25.0%
! AACAAACAAAATATCACATG+CGG - chr4_3:34981055-34981074 None:intergenic 25.0%
! AATAGTGCGAATATTTAGTA+AGG - chr4_3:34981190-34981209 None:intergenic 25.0%
ATCGGATTTAAATCAGGAAA+AGG - chr4_3:34978459-34978478 None:intergenic 30.0%
GAATTGTCACGGTTAAAAAT+CGG - chr4_3:34978477-34978496 None:intergenic 30.0%
!! GGTTTTGTATAATTCGTGTT+TGG + chr4_3:34978511-34978530 Msa0639310:intron 30.0%
!! TCACTGGTTTACTTGAATTT+AGG + chr4_3:34978540-34978559 Msa0639310:intron 30.0%
! GTTTACTTGAATTTAGGATC+AGG + chr4_3:34978546-34978565 Msa0639310:intron 30.0%
GTTATTCCTTTGTAGAGATT+TGG + chr4_3:34978778-34978797 Msa0639310:intron 30.0%
AACATTAGCACAAAGTTTGA+TGG + chr4_3:34978831-34978850 Msa0639310:intron 30.0%
AACATCCGATAGAAAGTTAA+GGG + chr4_3:34979107-34979126 Msa0639310:CDS 30.0%
TGTATCCCTTAACTTTCTAT+CGG - chr4_3:34979115-34979134 None:intergenic 30.0%
AGGGATACATTTGAAAAGTT+TGG + chr4_3:34979126-34979145 Msa0639310:CDS 30.0%
! ATTTGAGATATTGATCACCT+TGG - chr4_3:34979166-34979185 None:intergenic 30.0%
ATGCTATTGATGCTATGAAT+GGG + chr4_3:34979538-34979557 Msa0639310:CDS 30.0%
ATGAATGGGATAGATTTAGA+TGG + chr4_3:34979552-34979571 Msa0639310:CDS 30.0%
CTCTATCAACAGTAATAGTT+CGG - chr4_3:34979578-34979597 None:intergenic 30.0%
ATTAAATACCTCGATGAGAA+AGG - chr4_3:34979994-34980013 None:intergenic 30.0%
!! TTTGCAGCTTTGAAATCAAA+TGG + chr4_3:34980117-34980136 Msa0639310:intron 30.0%
!! TTGCAGCTTTGAAATCAAAT+GGG + chr4_3:34980118-34980137 Msa0639310:intron 30.0%
!!! GTGTTTTGATATGCAATACT+TGG + chr4_3:34980219-34980238 Msa0639310:three_prime_UTR 30.0%
TACCTTGATTCTATTTAGAG+AGG + chr4_3:34980467-34980486 Msa0639310:three_prime_UTR 30.0%
TTCCTCTCTAAATAGAATCA+AGG - chr4_3:34980472-34980491 None:intergenic 30.0%
GATTCTATTTAGAGAGGAAT+TGG + chr4_3:34980473-34980492 Msa0639310:three_prime_UTR 30.0%
AAGAAGCAAAACTCTACATT+TGG - chr4_3:34980552-34980571 None:intergenic 30.0%
!! GTCATTTTGGATTCATCAAT+GGG + chr4_3:34980767-34980786 Msa0639310:three_prime_UTR 30.0%
ATGCGTTATTGTTCTATTTG+GGG + chr4_3:34980999-34981018 Msa0639310:three_prime_UTR 30.0%
!! CAGTTTGTTGTGCTATAAAA+CGG + chr4_3:34981081-34981100 Msa0639310:three_prime_UTR 30.0%
! CTTGAATTTAGGATCAGGAT+AGG + chr4_3:34978551-34978570 Msa0639310:intron 35.0%
TCTTCTGTTTGTGTGTATTG+AGG + chr4_3:34978581-34978600 Msa0639310:intron 35.0%
TTTGTGTGTATTGAGGATTG+AGG + chr4_3:34978588-34978607 Msa0639310:intron 35.0%
TATGTGATAGCTGTGAAGTT+TGG + chr4_3:34978680-34978699 Msa0639310:intron 35.0%
TCCTTTGTAGAGATTTGGAA+AGG + chr4_3:34978783-34978802 Msa0639310:intron 35.0%
ACCTTTCCAAATCTCTACAA+AGG - chr4_3:34978787-34978806 None:intergenic 35.0%
GTGTTGAATTAATCAGTCCA+AGG - chr4_3:34978976-34978995 None:intergenic 35.0%
TTAATTCAACACCGTGTAGT+TGG + chr4_3:34978984-34979003 Msa0639310:intron 35.0%
! GTACATGGTGTTGAATTAGT+CGG - chr4_3:34979011-34979030 None:intergenic 35.0%
!! GATGAGTATCGTTGTTTTGT+TGG + chr4_3:34979069-34979088 Msa0639310:CDS 35.0%
CAACATCCGATAGAAAGTTA+AGG + chr4_3:34979106-34979125 Msa0639310:CDS 35.0%
TCATGTTTGTACCTAGAAGT+TGG + chr4_3:34979207-34979226 Msa0639310:intron 35.0%
TACAGCATAAACCAACTTCT+AGG - chr4_3:34979221-34979240 None:intergenic 35.0%
GATGCTATTGATGCTATGAA+TGG + chr4_3:34979537-34979556 Msa0639310:CDS 35.0%
!! TAAACCTGGTCATTTTGCTA+GGG + chr4_3:34979725-34979744 Msa0639310:CDS 35.0%
TTGATATACCTTTCTCATCG+AGG + chr4_3:34979983-34980002 Msa0639310:exon 35.0%
TTCGTTCACAATGTTCTTCT+TGG + chr4_3:34980167-34980186 Msa0639310:three_prime_UTR 35.0%
! GTTCTTCTTGGATTGTTGTT+TGG + chr4_3:34980179-34980198 Msa0639310:three_prime_UTR 35.0%
!! TTCTTGGATTGTTGTTTGGA+TGG + chr4_3:34980183-34980202 Msa0639310:three_prime_UTR 35.0%
ACTAATTAACGTTCCTATGC+TGG + chr4_3:34980254-34980273 Msa0639310:three_prime_UTR 35.0%
ATGCTATCAATAATTGCTGC+TGG + chr4_3:34980339-34980358 Msa0639310:three_prime_UTR 35.0%
! AGAGGAATTGGAATGTTTTG+TGG + chr4_3:34980485-34980504 Msa0639310:three_prime_UTR 35.0%
! ATTGGAATGTTTTGTGGCTT+TGG + chr4_3:34980491-34980510 Msa0639310:three_prime_UTR 35.0%
!!! TTTTGTGGCTTTGGATGTTT+GGG + chr4_3:34980500-34980519 Msa0639310:three_prime_UTR 35.0%
!!! GGTTTTTTCATGTACCAAGA+TGG + chr4_3:34980522-34980541 Msa0639310:three_prime_UTR 35.0%
! TTTTTCATGTACCAAGATGG+AGG + chr4_3:34980525-34980544 Msa0639310:three_prime_UTR 35.0%
! AGAGTTTTGCTTCTTCTCAT+TGG + chr4_3:34980557-34980576 Msa0639310:three_prime_UTR 35.0%
!! TATCCTGTTTCTGGATCTTT+GGG + chr4_3:34980616-34980635 Msa0639310:three_prime_UTR 35.0%
! TTGGCTAAATGTGGTCATTT+TGG + chr4_3:34980754-34980773 Msa0639310:three_prime_UTR 35.0%
!! GGTCATTTTGGATTCATCAA+TGG + chr4_3:34980766-34980785 Msa0639310:three_prime_UTR 35.0%
CATGTCCAGTTAGCAATAAA+GGG - chr4_3:34980794-34980813 None:intergenic 35.0%
ACATGTCCAGTTAGCAATAA+AGG - chr4_3:34980795-34980814 None:intergenic 35.0%
! AAAATCTTTTCAGCTGCAAG+AGG + chr4_3:34980838-34980857 Msa0639310:three_prime_UTR 35.0%
TCTTCATGGGGAATATATGA+AGG + chr4_3:34980901-34980920 Msa0639310:three_prime_UTR 35.0%
CTTCATGGGGAATATATGAA+GGG + chr4_3:34980902-34980921 Msa0639310:three_prime_UTR 35.0%
TTCATGGGGAATATATGAAG+GGG + chr4_3:34980903-34980922 Msa0639310:three_prime_UTR 35.0%
TGTGCCAGAATCATAGAAAA+CGG - chr4_3:34980932-34980951 None:intergenic 35.0%
TATGATTCTGGCACATTATG+TGG + chr4_3:34980937-34980956 Msa0639310:three_prime_UTR 35.0%
TGCGTTATTGTTCTATTTGG+GGG + chr4_3:34981000-34981019 Msa0639310:three_prime_UTR 35.0%
! CGTTTTATAGCACAACAAAC+TGG - chr4_3:34981083-34981102 None:intergenic 35.0%
TTCGCACTATTTGTCTATCA+TGG + chr4_3:34981199-34981218 Msa0639310:three_prime_UTR 35.0%
GAACAAACCACATTTCTATG+TGG + chr4_3:34981391-34981410 Msa0639310:three_prime_UTR 35.0%
ACAATTCGCTCAGGAACATT+AGG + chr4_3:34978490-34978509 Msa0639310:intron 40.0%
! CATACTATTGTGCTGCTGTT+TGG + chr4_3:34978895-34978914 Msa0639310:intron 40.0%
AATTAGTCGGTCCAACTACA+CGG - chr4_3:34978998-34979017 None:intergenic 40.0%
!! GAGTATCGTTGTTTTGTTGG+TGG + chr4_3:34979072-34979091 Msa0639310:CDS 40.0%
AAAGTTTGGCAAGCTTACAG+AGG + chr4_3:34979140-34979159 Msa0639310:CDS 40.0%
GTGGTTGTTGACAAATTCTC+TGG + chr4_3:34979462-34979481 Msa0639310:CDS 40.0%
TGCTTCAATTGTGGTAAACC+TGG + chr4_3:34979711-34979730 Msa0639310:CDS 40.0%
!! GTAAACCTGGTCATTTTGCT+AGG + chr4_3:34979724-34979743 Msa0639310:CDS 40.0%
GGTGGTAGAGAAAGTAGATA+TGG + chr4_3:34979792-34979811 Msa0639310:CDS 40.0%
GGTAGAGAAAGTAGATATGG+TGG + chr4_3:34979795-34979814 Msa0639310:CDS 40.0%
! GAAAGTAGATATGGTGGAAG+TGG + chr4_3:34979801-34979820 Msa0639310:CDS 40.0%
CACTATGGTCCAGATAGAAA+TGG + chr4_3:34979834-34979853 Msa0639310:CDS 40.0%
ACAAACCAAAAGACCAGCAT+AGG - chr4_3:34980270-34980289 None:intergenic 40.0%
! TTTATGCATGTCGAGTTGCA+TGG + chr4_3:34980427-34980446 Msa0639310:three_prime_UTR 40.0%
ATAGAATCAAGGTAATCCCG+CGG - chr4_3:34980461-34980480 None:intergenic 40.0%
!!! GTTTTGTGGCTTTGGATGTT+TGG + chr4_3:34980499-34980518 Msa0639310:three_prime_UTR 40.0%
!! TTTGTGGCTTTGGATGTTTG+GGG + chr4_3:34980501-34980520 Msa0639310:three_prime_UTR 40.0%
TTCTTCTCATTGGAAGAACG+AGG + chr4_3:34980567-34980586 Msa0639310:three_prime_UTR 40.0%
CAGAAACAGGATAGCCAAAT+CGG - chr4_3:34980609-34980628 None:intergenic 40.0%
!! CTATCCTGTTTCTGGATCTT+TGG + chr4_3:34980615-34980634 Msa0639310:three_prime_UTR 40.0%
TTTCCCAAAGATCCAGAAAC+AGG - chr4_3:34980622-34980641 None:intergenic 40.0%
! TCTTTGGGAAAGTAGTCAAG+CGG + chr4_3:34980631-34980650 Msa0639310:three_prime_UTR 40.0%
ACTTCACCATCACTAGAGTT+AGG + chr4_3:34980685-34980704 Msa0639310:intron 40.0%
CTGAAACCTAACTCTAGTGA+TGG - chr4_3:34980694-34980713 None:intergenic 40.0%
! GATGTTATTGAGCTTTTGGC+AGG + chr4_3:34980727-34980746 Msa0639310:intron 40.0%
!!! TACCGCTCTTGTTTTCTTCA+TGG + chr4_3:34980887-34980906 Msa0639310:three_prime_UTR 40.0%
!!! ACCGCTCTTGTTTTCTTCAT+GGG + chr4_3:34980888-34980907 Msa0639310:three_prime_UTR 40.0%
TCATGGGGAATATATGAAGG+GGG + chr4_3:34980904-34980923 Msa0639310:three_prime_UTR 40.0%
! GGTTCCGTTTTCTATGATTC+TGG + chr4_3:34980925-34980944 Msa0639310:three_prime_UTR 40.0%
TATCACATGCGGCAGTTAAA+AGG - chr4_3:34981044-34981063 None:intergenic 40.0%
GTGGAAGAGAATGGAAGTTT+AGG - chr4_3:34981127-34981146 None:intergenic 40.0%
ATGGTTGAGTTCACTAGCAT+TGG + chr4_3:34981150-34981169 Msa0639310:three_prime_UTR 40.0%
CAAACCACATTTCTATGTGG+TGG + chr4_3:34981394-34981413 Msa0639310:three_prime_UTR 40.0%
AACTCCACCACATAGAAATG+TGG - chr4_3:34981401-34981420 None:intergenic 40.0%
! GAAAATTTTCCTGCAAGCTC+TGG - chr4_3:34981430-34981449 None:intergenic 40.0%
!! TGGAAGAGTACCGTGAGTTA+GGG - chr4_3:34978377-34978396 None:intergenic 45.0%
!! TTGGAAGAGTACCGTGAGTT+AGG - chr4_3:34978378-34978397 None:intergenic 45.0%
TTAACCGTGACAATTCGCTC+AGG + chr4_3:34978481-34978500 Msa0639310:intron 45.0%
TGTTCCTGAGCGAATTGTCA+CGG - chr4_3:34978488-34978507 None:intergenic 45.0%
TCGTGTTTGGGAAACATCAC+TGG + chr4_3:34978524-34978543 Msa0639310:intron 45.0%
! GCAGTTTAAAATGTCGGACG+AGG + chr4_3:34979047-34979066 Msa0639310:intron 45.0%
TATCGGATGTTGACCAAGCA+AGG - chr4_3:34979098-34979117 None:intergenic 45.0%
! TCTCTAGAGGATCCTTGTTG+AGG - chr4_3:34979606-34979625 None:intergenic 45.0%
! CAAGGATCCTCTAGAGATGA+TGG + chr4_3:34979609-34979628 Msa0639310:CDS 45.0%
GATGGAGGATGCTTCAATTG+TGG + chr4_3:34979702-34979721 Msa0639310:CDS 45.0%
! AGTAGATATGGTGGAAGTGG+TGG + chr4_3:34979804-34979823 Msa0639310:CDS 45.0%
GAACGGTCTCCATTTCTATC+TGG - chr4_3:34979846-34979865 None:intergenic 45.0%
AGAAATGGAGACCGTTCTTC+TGG + chr4_3:34979849-34979868 Msa0639310:CDS 45.0%
!!! ACGTTCCTATGCTGGTCTTT+TGG + chr4_3:34980262-34980281 Msa0639310:three_prime_UTR 45.0%
! GTTGCATGGCATATTTTCCG+CGG + chr4_3:34980441-34980460 Msa0639310:three_prime_UTR 45.0%
! TTGCATGGCATATTTTCCGC+GGG + chr4_3:34980442-34980461 Msa0639310:three_prime_UTR 45.0%
CTACATTTGGACCTCCATCT+TGG - chr4_3:34980539-34980558 None:intergenic 45.0%
! CGATTTGGCTATCCTGTTTC+TGG + chr4_3:34980607-34980626 Msa0639310:three_prime_UTR 45.0%
! CTTTGGGAAAGTAGTCAAGC+GGG + chr4_3:34980632-34980651 Msa0639310:three_prime_UTR 45.0%
! TGAGCTTTTGGCAGGATTCT+TGG + chr4_3:34980735-34980754 Msa0639310:intron 45.0%
GCAGGATTCTTGGCTAAATG+TGG + chr4_3:34980745-34980764 Msa0639310:intron 45.0%
TGGGTCCCTTTATTGCTAAC+TGG + chr4_3:34980786-34980805 Msa0639310:three_prime_UTR 45.0%
CCCCATGAAGAAAACAAGAG+CGG - chr4_3:34980892-34980911 None:intergenic 45.0%
!!! CCGCTCTTGTTTTCTTCATG+GGG + chr4_3:34980889-34980908 Msa0639310:three_prime_UTR 45.0%
! GAGAATGGAAGTTTAGGACC+AGG - chr4_3:34981121-34981140 None:intergenic 45.0%
TTCCATTCTCTTCCACGACA+TGG + chr4_3:34981131-34981150 Msa0639310:three_prime_UTR 45.0%
AACCATGTCGTGGAAGAGAA+TGG - chr4_3:34981136-34981155 None:intergenic 45.0%
TAGTGAACTCAACCATGTCG+TGG - chr4_3:34981146-34981165 None:intergenic 45.0%
!! AAGAGTACCGTGAGTTAGGG+CGG - chr4_3:34978374-34978393 None:intergenic 50.0%
!!! GTTTTGTTGGTGGCCTTGCT+TGG + chr4_3:34979082-34979101 Msa0639310:CDS 50.0%
AAATTCTCTGGACGCTCTCG+TGG + chr4_3:34979474-34979493 Msa0639310:CDS 50.0%
GATAGAGCTCAGCCTCAACA+AGG + chr4_3:34979591-34979610 Msa0639310:CDS 50.0%
GCGATCACCATCATCTCTAG+AGG - chr4_3:34979619-34979638 None:intergenic 50.0%
GAACGTGATCGAGATTACCG+AGG + chr4_3:34979663-34979682 Msa0639310:CDS 50.0%
CATTAGATCCTCGACCACCT+CGG - chr4_3:34979683-34979702 None:intergenic 50.0%
GGTGGTCGAGGATCTAATGA+TGG + chr4_3:34979684-34979703 Msa0639310:CDS 50.0%
GGTCGAGGATCTAATGATGG+AGG + chr4_3:34979687-34979706 Msa0639310:CDS 50.0%
CACTCCCTAGCAAAATGACC+AGG - chr4_3:34979732-34979751 None:intergenic 50.0%
! AGATATGGTGGAAGTGGTGG+TGG + chr4_3:34979807-34979826 Msa0639310:CDS 50.0%
AATGGAGACCGTTCTTCTGG+CGG + chr4_3:34979852-34979871 Msa0639310:CDS 50.0%
TATCGTGATCGTGATCGTGC+TGG + chr4_3:34979924-34979943 Msa0639310:CDS 50.0%
CCAGATCCACGTCGTTCATA+TGG - chr4_3:34979951-34979970 None:intergenic 50.0%
CCATATGAACGACGTGGATC+TGG + chr4_3:34979948-34979967 Msa0639310:CDS 50.0%
TATGAACGACGTGGATCTGG+AGG + chr4_3:34979951-34979970 Msa0639310:CDS 50.0%
CTGCTCTGCTCTCTCTCTCT+AGG - chr4_3:34978339-34978358 None:intergenic 55.0%
CTAGAGAGAGAGAGCAGAGC+AGG + chr4_3:34978337-34978356 Msa0639310:five_prime_UTR 55.0%
CCAAGTTCCGCCCTAACTCA+CGG + chr4_3:34978364-34978383 Msa0639310:five_prime_UTR 55.0%
TGGCAAGCTTACAGAGGCCA+AGG + chr4_3:34979146-34979165 Msa0639310:CDS 55.0%
! TCTGGACGCTCTCGTGGTTT+TGG + chr4_3:34979480-34979499 Msa0639310:CDS 55.0%
CGTGATCGAGATTACCGAGG+TGG + chr4_3:34979666-34979685 Msa0639310:CDS 55.0%
!! TTTGCTAGGGAGTGCCCTAG+TGG + chr4_3:34979738-34979757 Msa0639310:CDS 55.0%
CTCTTCCCCCTTCACCACTA+GGG - chr4_3:34979755-34979774 None:intergenic 55.0%
CTAGTGGTGAAGGGGGAAGA+GGG + chr4_3:34979754-34979773 Msa0639310:CDS 55.0%
TAGTGGTGAAGGGGGAAGAG+GGG + chr4_3:34979755-34979774 Msa0639310:CDS 55.0%
!! TATGGTGGAAGTGGTGGTGG+TGG + chr4_3:34979810-34979829 Msa0639310:CDS 55.0%
!! AGTGGTGGTGGTGGTCACTA+TGG + chr4_3:34979819-34979838 Msa0639310:CDS 55.0%
GGTGGTAGTCGTGGAGATTC+TGG + chr4_3:34979888-34979907 Msa0639310:CDS 55.0%
GCTGGACCATATGAACGACG+TGG + chr4_3:34979942-34979961 Msa0639310:CDS 55.0%
! CATGCATGTCGAGTGCTGCT+TGG + chr4_3:34980384-34980403 Msa0639310:three_prime_UTR 55.0%
ACGGTAGTCAAACCTGAGCC+TGG + chr4_3:34981100-34981119 Msa0639310:three_prime_UTR 55.0%
! GGAAGTTTAGGACCAGGCTC+AGG - chr4_3:34981115-34981134 None:intergenic 55.0%
CCGTGAGTTAGGGCGGAACT+TGG - chr4_3:34978367-34978386 None:intergenic 60.0%
CGAGATTACCGAGGTGGTCG+AGG + chr4_3:34979672-34979691 Msa0639310:CDS 60.0%
!! AGGGAGTGCCCTAGTGGTGA+AGG + chr4_3:34979744-34979763 Msa0639310:CDS 60.0%
!! GGGAGTGCCCTAGTGGTGAA+GGG + chr4_3:34979745-34979764 Msa0639310:CDS 60.0%
! GGAGTGCCCTAGTGGTGAAG+GGG + chr4_3:34979746-34979765 Msa0639310:CDS 60.0%
GAGTGCCCTAGTGGTGAAGG+GGG + chr4_3:34979747-34979766 Msa0639310:CDS 60.0%
CCTCTTCCCCCTTCACCACT+AGG - chr4_3:34979756-34979775 None:intergenic 60.0%
CCTAGTGGTGAAGGGGGAAG+AGG + chr4_3:34979753-34979772 Msa0639310:CDS 60.0%
AGTGGTGAAGGGGGAAGAGG+GGG + chr4_3:34979756-34979775 Msa0639310:CDS 60.0%
TGCTGCGACCGCCAGAAGAA+CGG - chr4_3:34979863-34979882 None:intergenic 60.0%
TTCTTCTGGCGGTCGCAGCA+GGG + chr4_3:34979863-34979882 Msa0639310:CDS 60.0%
! AGCAGGGATGGTGGTAGTCG+TGG + chr4_3:34979879-34979898 Msa0639310:CDS 60.0%
GAACGACGTGGATCTGGAGG+CGG + chr4_3:34979954-34979973 Msa0639310:CDS 60.0%
GTTCGTGTGCCAGAGCTTGC+AGG + chr4_3:34981418-34981437 Msa0639310:three_prime_UTR 60.0%
GGTGATCGCTACCGAGAGCG+TGG + chr4_3:34979630-34979649 Msa0639310:CDS 65.0%
GTGAAGGGGGAAGAGGGGGA+GGG + chr4_3:34979760-34979779 Msa0639310:CDS 65.0%
TGAAGGGGGAAGAGGGGGAG+GGG + chr4_3:34979761-34979780 Msa0639310:CDS 65.0%
AGAGGGGGAGGGGGAAGGTA+TGG + chr4_3:34979771-34979790 Msa0639310:CDS 65.0%
GTTCTTCTGGCGGTCGCAGC+AGG + chr4_3:34979862-34979881 Msa0639310:CDS 65.0%
CGCGATCGCGACCACGCTCT+CGG - chr4_3:34979644-34979663 None:intergenic 70.0%
GGTGAAGGGGGAAGAGGGGG+AGG + chr4_3:34979759-34979778 Msa0639310:CDS 70.0%
GAAGGGGGAAGAGGGGGAGG+GGG + chr4_3:34979762-34979781 Msa0639310:CDS 70.0%
! GGGGGAGGGGGAAGGTATGG+TGG + chr4_3:34979774-34979793 Msa0639310:CDS 70.0%
TCTGGCGGTCGCAGCAGGGA+TGG + chr4_3:34979867-34979886 Msa0639310:CDS 70.0%
GGGGAAGAGGGGGAGGGGGA+AGG + chr4_3:34979766-34979785 Msa0639310:CDS 75.0%
GGCGGTCGCAGCAGGGATGG+TGG + chr4_3:34979870-34979889 Msa0639310:CDS 75.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 34978323 34981459 34978323 ID=Msa0639310;Name=Msa0639310
chr4_3 mRNA 34978323 34981459 34978323 ID=Msa0639310-mRNA-1;Parent=Msa0639310;Name=Msa0639310-mRNA-1;_AED=0.09;_eAED=0.09;_QI=69|1|1|1|0.5|0.4|5|1259|211
chr4_3 exon 34978323 34978385 34978323 ID=Msa0639310-mRNA-1:exon:8193;Parent=Msa0639310-mRNA-1
chr4_3 exon 34979051 34979167 34979051 ID=Msa0639310-mRNA-1:exon:8194;Parent=Msa0639310-mRNA-1
chr4_3 exon 34979462 34980004 34979462 ID=Msa0639310-mRNA-1:exon:8195;Parent=Msa0639310-mRNA-1
chr4_3 exon 34980124 34980653 34980124 ID=Msa0639310-mRNA-1:exon:8196;Parent=Msa0639310-mRNA-1
chr4_3 exon 34980749 34981459 34980749 ID=Msa0639310-mRNA-1:exon:8197;Parent=Msa0639310-mRNA-1
chr4_3 five_prime_UTR 34978323 34978385 34978323 ID=Msa0639310-mRNA-1:five_prime_utr;Parent=Msa0639310-mRNA-1
chr4_3 five_prime_UTR 34979051 34979056 34979051 ID=Msa0639310-mRNA-1:five_prime_utr;Parent=Msa0639310-mRNA-1
chr4_3 CDS 34979057 34979167 34979057 ID=Msa0639310-mRNA-1:cds;Parent=Msa0639310-mRNA-1
chr4_3 CDS 34979462 34979986 34979462 ID=Msa0639310-mRNA-1:cds;Parent=Msa0639310-mRNA-1
chr4_3 three_prime_UTR 34979987 34980004 34979987 ID=Msa0639310-mRNA-1:three_prime_utr;Parent=Msa0639310-mRNA-1
chr4_3 three_prime_UTR 34980124 34980653 34980124 ID=Msa0639310-mRNA-1:three_prime_utr;Parent=Msa0639310-mRNA-1
chr4_3 three_prime_UTR 34980749 34981459 34980749 ID=Msa0639310-mRNA-1:three_prime_utr;Parent=Msa0639310-mRNA-1
Gene Sequence

>Msa0639310

ATGTCGGACGAGGATGAGTATCGTTGTTTTGTTGGTGGCCTTGCTTGGTCAACATCCGATAGAAAGTTAAGGGATACATTTGAAAAGTTTGGCAAGCTTACAGAGGCCAAGGTGGTTGTTGACAAATTCTCTGGACGCTCTCGTGGTTTTGGATTTGTCACATATGATGAAAAGAAAGCAATGGAAGATGCTATTGATGCTATGAATGGGATAGATTTAGATGGCCGAACTATTACTGTTGATAGAGCTCAGCCTCAACAAGGATCCTCTAGAGATGATGGTGATCGCTACCGAGAGCGTGGTCGCGATCGCGAACGTGATCGAGATTACCGAGGTGGTCGAGGATCTAATGATGGAGGATGCTTCAATTGTGGTAAACCTGGTCATTTTGCTAGGGAGTGCCCTAGTGGTGAAGGGGGAAGAGGGGGAGGGGGAAGGTATGGTGGTAGAGAAAGTAGATATGGTGGAAGTGGTGGTGGTGGTCACTATGGTCCAGATAGAAATGGAGACCGTTCTTCTGGCGGTCGCAGCAGGGATGGTGGTAGTCGTGGAGATTCTGGAAGTGATCGATACTATCGTGATCGTGATCGTGCTGGACCATATGAACGACGTGGATCTGGAGGCGGCTATCGTTGA
Protein sequence

>Msa0639310

MSDEDEYRCFVGGLAWSTSDRKLRDTFEKFGKLTEAKVVVDKFSGRSRGFGFVTYDEKKAMEDAIDAMNGIDLDGRTITVDRAQPQQGSSRDDGDRYRERGRDRERDRDYRGGRGSNDGGCFNCGKPGHFARECPSGEGGRGGGGRYGGRESRYGGSGGGGHYGPDRNGDRSSGGRSRDGGSRGDSGSDRYYRDRDRAGPYERRGSGGGYR*