AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1329300


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1329300 MtrunA17_Chr4g0056141 100.000 62 0 0 1 62 1 62 2.23e-39 123
Msa1329300 MtrunA17_Chr5g0399231 100.000 62 0 0 1 62 1 62 2.23e-39 123
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1329300 AT2G19750.1 95.161 62 3 0 1 62 1 62 6.52e-30 100
Msa1329300 AT5G56670.1 95.161 62 3 0 1 62 1 62 6.52e-30 100
Msa1329300 AT4G29390.1 95.161 62 3 0 1 62 1 62 6.52e-30 100

Find 32 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTCTTGCTTTGCAACTTT+TGG 0.146380 8_4:+47826897 None:intergenic
ACAAATAGATCGAGCATTTC+TGG 0.201747 8_4:-47827160 Msa1329300:five_prime_UTR
GCAAATAATTTGACCTTTGC+TGG 0.248572 8_4:-47826407 Msa1329300:three_prime_UTR
CATTTCTGGTTTGAGTTTCT+AGG 0.249983 8_4:-47827146 Msa1329300:five_prime_UTR
TTTGTATTGGCAGGGGTTAA+AGG 0.327284 8_4:+47827178 None:intergenic
TATTCGACCATATGATTGTT+TGG 0.335567 8_4:-47826491 Msa1329300:three_prime_UTR
TTGCTCGTGCTGGAAAAGTT+AGG 0.361428 8_4:-47826929 Msa1329300:CDS
CTCGATCTATTTGTATTGGC+AGG 0.369976 8_4:+47827169 None:intergenic
ATTATTGCAGTTGTGGGATT+CGG 0.384343 8_4:-47826689 Msa1329300:intron
AATGCTCGATCTATTTGTAT+TGG 0.404236 8_4:+47827165 None:intergenic
GACAAATTCGTCTTCACAAT+GGG 0.434236 8_4:-47827069 Msa1329300:intron
ATTTCTGGTTTGAGTTTCTA+GGG 0.438640 8_4:-47827145 Msa1329300:five_prime_UTR
TGCTCGTGCTGGAAAAGTTA+GGG 0.448013 8_4:-47826928 Msa1329300:CDS
CATGGTTCCCTTGCTCGTGC+TGG 0.450019 8_4:-47826939 Msa1329300:CDS
TTTATTTGATTCATGAATTG+TGG 0.463970 8_4:-47826584 Msa1329300:three_prime_UTR
CGATTTGCAGCAGCAGCTCT+CGG 0.470283 8_4:+47827116 None:intergenic
CAAATAATTTGACCTTTGCT+GGG 0.477286 8_4:-47826406 Msa1329300:three_prime_UTR
AAAGTGAAGATGATGCTTAG+TGG 0.503837 8_4:-47826639 Msa1329300:exon
CGATCTATTTGTATTGGCAG+GGG 0.524264 8_4:+47827171 None:intergenic
TCGATCTATTTGTATTGGCA+GGG 0.525257 8_4:+47827170 None:intergenic
AGTTACCGGCGGTGACGAAA+CGG 0.526875 8_4:+47826826 None:intergenic
CGCTTGTGAGCACGGCCTCG+TGG 0.529866 8_4:+47826864 None:intergenic
ATTGCATACGCTTGTGAGCA+CGG 0.548283 8_4:+47826856 None:intergenic
AATTACAAAATGGGTTGGTG+TGG 0.553999 8_4:-47826463 Msa1329300:three_prime_UTR
TGAAATGCAGGTAAAGTGCA+TGG 0.568662 8_4:-47826957 Msa1329300:intron
TGACAAATTCGTCTTCACAA+TGG 0.586924 8_4:-47827070 Msa1329300:exon
GCTCGTGCTGGAAAAGTTAG+GGG 0.589768 8_4:-47826927 Msa1329300:CDS
TTTATGCACAATTTGTACGT+GGG 0.604062 8_4:-47826317 Msa1329300:three_prime_UTR
GTTTATGCACAATTTGTACG+TGG 0.618169 8_4:-47826318 Msa1329300:three_prime_UTR
AATCGCCGTTTCGTCACCGC+CGG 0.622871 8_4:-47826831 Msa1329300:intron
GTGGGATTCGGAAAGAAGCG+TGG 0.638925 8_4:-47826677 Msa1329300:CDS
GACAAGAAGAAGCAGCCACG+AGG 0.675762 8_4:-47826879 Msa1329300:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTGTTCTGATTT+TGG - chr8_4:47826746-47826765 Msa1329300:intron 15.0%
!!! TTTGGTTTTTAATTACAAAA+TGG - chr8_4:47826997-47827016 Msa1329300:intron 15.0%
!!! TTGGTTTTTAATTACAAAAT+GGG - chr8_4:47826998-47827017 Msa1329300:intron 15.0%
!!! TTTTTTTTTTGTTCTGATTT+TGG - chr8_4:47826746-47826765 Msa1329300:intron 15.0%
!!! TTTGGTTTTTAATTACAAAA+TGG - chr8_4:47826997-47827016 Msa1329300:intron 15.0%
!!! TTGGTTTTTAATTACAAAAT+GGG - chr8_4:47826998-47827017 Msa1329300:intron 15.0%
!! TGATACGATTTCTATAAAAT+TGG - chr8_4:47826440-47826459 Msa1329300:three_prime_UTR 20.0%
!! TTTATTTGATTCATGAATTG+TGG - chr8_4:47826886-47826905 Msa1329300:CDS 20.0%
!! TTAAAAACCAAACAATCATA+TGG + chr8_4:47826989-47827008 None:intergenic 20.0%
!!! TTTTTAATTACAAAATGGGT+TGG - chr8_4:47827002-47827021 Msa1329300:intron 20.0%
!! TGATACGATTTCTATAAAAT+TGG - chr8_4:47826440-47826459 Msa1329300:three_prime_UTR 20.0%
!! TTTATTTGATTCATGAATTG+TGG - chr8_4:47826886-47826905 Msa1329300:CDS 20.0%
!! TTAAAAACCAAACAATCATA+TGG + chr8_4:47826989-47827008 None:intergenic 20.0%
!!! TTTTTAATTACAAAATGGGT+TGG - chr8_4:47827002-47827021 Msa1329300:intron 20.0%
! AGAAAGAATGATAAAGTTAC+CGG + chr8_4:47826661-47826680 None:intergenic 25.0%
! TATGATTATTATTGCAGTTG+TGG - chr8_4:47826774-47826793 Msa1329300:intron 25.0%
! ATGATTATTATTGCAGTTGT+GGG - chr8_4:47826775-47826794 Msa1329300:intron 25.0%
!!! TTCTTTGTTTTAAGACATTG+AGG - chr8_4:47827113-47827132 Msa1329300:five_prime_UTR 25.0%
! AGAAAGAATGATAAAGTTAC+CGG + chr8_4:47826661-47826680 None:intergenic 25.0%
! TATGATTATTATTGCAGTTG+TGG - chr8_4:47826774-47826793 Msa1329300:intron 25.0%
! ATGATTATTATTGCAGTTGT+GGG - chr8_4:47826775-47826794 Msa1329300:intron 25.0%
!!! TTCTTTGTTTTAAGACATTG+AGG - chr8_4:47827113-47827132 Msa1329300:five_prime_UTR 25.0%
AATGCTCGATCTATTTGTAT+TGG + chr8_4:47826308-47826327 None:intergenic 30.0%
!! ATTTCTGGTTTGAGTTTCTA+GGG - chr8_4:47826325-47826344 Msa1329300:three_prime_UTR 30.0%
! TGAATCGAAATTTGAAATGC+AGG - chr8_4:47826501-47826520 Msa1329300:three_prime_UTR 30.0%
!! TGAAAGAATTTTGTTGTGCT+AGG - chr8_4:47826936-47826955 Msa1329300:CDS 30.0%
TATTCGACCATATGATTGTT+TGG - chr8_4:47826979-47826998 Msa1329300:intron 30.0%
CAAATAATTTGACCTTTGCT+GGG - chr8_4:47827064-47827083 Msa1329300:intron 30.0%
TTTATGCACAATTTGTACGT+GGG - chr8_4:47827153-47827172 Msa1329300:five_prime_UTR 30.0%
AATGCTCGATCTATTTGTAT+TGG + chr8_4:47826308-47826327 None:intergenic 30.0%
!! ATTTCTGGTTTGAGTTTCTA+GGG - chr8_4:47826325-47826344 Msa1329300:three_prime_UTR 30.0%
! TGAATCGAAATTTGAAATGC+AGG - chr8_4:47826501-47826520 Msa1329300:three_prime_UTR 30.0%
!! TGAAAGAATTTTGTTGTGCT+AGG - chr8_4:47826936-47826955 Msa1329300:CDS 30.0%
TATTCGACCATATGATTGTT+TGG - chr8_4:47826979-47826998 Msa1329300:intron 30.0%
CAAATAATTTGACCTTTGCT+GGG - chr8_4:47827064-47827083 Msa1329300:intron 30.0%
TTTATGCACAATTTGTACGT+GGG - chr8_4:47827153-47827172 Msa1329300:five_prime_UTR 30.0%
! TCGATCTATTTGTATTGGCA+GGG + chr8_4:47826303-47826322 None:intergenic 35.0%
ACAAATAGATCGAGCATTTC+TGG - chr8_4:47826310-47826329 Msa1329300:three_prime_UTR 35.0%
!! CATTTCTGGTTTGAGTTTCT+AGG - chr8_4:47826324-47826343 Msa1329300:three_prime_UTR 35.0%
TGACAAATTCGTCTTCACAA+TGG - chr8_4:47826400-47826419 Msa1329300:three_prime_UTR 35.0%
GACAAATTCGTCTTCACAAT+GGG - chr8_4:47826401-47826420 Msa1329300:three_prime_UTR 35.0%
! TGTCTTGCTTTGCAACTTTT+GGG + chr8_4:47826575-47826594 None:intergenic 35.0%
! TTGTCTTGCTTTGCAACTTT+TGG + chr8_4:47826576-47826595 None:intergenic 35.0%
AAGAATGATAAAGTTACCGG+CGG + chr8_4:47826658-47826677 None:intergenic 35.0%
ATTATTGCAGTTGTGGGATT+CGG - chr8_4:47826781-47826800 Msa1329300:intron 35.0%
!! CTTCACTTTTCAGATGAGTT+GGG + chr8_4:47826820-47826839 None:intergenic 35.0%
!! TCTTCACTTTTCAGATGAGT+TGG + chr8_4:47826821-47826840 None:intergenic 35.0%
AAAGTGAAGATGATGCTTAG+TGG - chr8_4:47826831-47826850 Msa1329300:intron 35.0%
!! AATTACAAAATGGGTTGGTG+TGG - chr8_4:47827007-47827026 Msa1329300:intron 35.0%
GCAAATAATTTGACCTTTGC+TGG - chr8_4:47827063-47827082 Msa1329300:intron 35.0%
! ACAACAAATTTTCCCAGCAA+AGG + chr8_4:47827079-47827098 None:intergenic 35.0%
GTTTATGCACAATTTGTACG+TGG - chr8_4:47827152-47827171 Msa1329300:five_prime_UTR 35.0%
! TCGATCTATTTGTATTGGCA+GGG + chr8_4:47826303-47826322 None:intergenic 35.0%
ACAAATAGATCGAGCATTTC+TGG - chr8_4:47826310-47826329 Msa1329300:three_prime_UTR 35.0%
!! CATTTCTGGTTTGAGTTTCT+AGG - chr8_4:47826324-47826343 Msa1329300:three_prime_UTR 35.0%
TGACAAATTCGTCTTCACAA+TGG - chr8_4:47826400-47826419 Msa1329300:three_prime_UTR 35.0%
GACAAATTCGTCTTCACAAT+GGG - chr8_4:47826401-47826420 Msa1329300:three_prime_UTR 35.0%
! TGTCTTGCTTTGCAACTTTT+GGG + chr8_4:47826575-47826594 None:intergenic 35.0%
! TTGTCTTGCTTTGCAACTTT+TGG + chr8_4:47826576-47826595 None:intergenic 35.0%
AAGAATGATAAAGTTACCGG+CGG + chr8_4:47826658-47826677 None:intergenic 35.0%
ATTATTGCAGTTGTGGGATT+CGG - chr8_4:47826781-47826800 Msa1329300:intron 35.0%
!! CTTCACTTTTCAGATGAGTT+GGG + chr8_4:47826820-47826839 None:intergenic 35.0%
!! TCTTCACTTTTCAGATGAGT+TGG + chr8_4:47826821-47826840 None:intergenic 35.0%
AAAGTGAAGATGATGCTTAG+TGG - chr8_4:47826831-47826850 Msa1329300:intron 35.0%
!! AATTACAAAATGGGTTGGTG+TGG - chr8_4:47827007-47827026 Msa1329300:intron 35.0%
GCAAATAATTTGACCTTTGC+TGG - chr8_4:47827063-47827082 Msa1329300:intron 35.0%
! ACAACAAATTTTCCCAGCAA+AGG + chr8_4:47827079-47827098 None:intergenic 35.0%
GTTTATGCACAATTTGTACG+TGG - chr8_4:47827152-47827171 Msa1329300:five_prime_UTR 35.0%
! TTTGTATTGGCAGGGGTTAA+AGG + chr8_4:47826295-47826314 None:intergenic 40.0%
! CGATCTATTTGTATTGGCAG+GGG + chr8_4:47826302-47826321 None:intergenic 40.0%
! CTCGATCTATTTGTATTGGC+AGG + chr8_4:47826304-47826323 None:intergenic 40.0%
TGAAATGCAGGTAAAGTGCA+TGG - chr8_4:47826513-47826532 Msa1329300:three_prime_UTR 40.0%
! GTCTTGCTTTGCAACTTTTG+GGG + chr8_4:47826574-47826593 None:intergenic 40.0%
! TTTGTATTGGCAGGGGTTAA+AGG + chr8_4:47826295-47826314 None:intergenic 40.0%
! CGATCTATTTGTATTGGCAG+GGG + chr8_4:47826302-47826321 None:intergenic 40.0%
! CTCGATCTATTTGTATTGGC+AGG + chr8_4:47826304-47826323 None:intergenic 40.0%
TGAAATGCAGGTAAAGTGCA+TGG - chr8_4:47826513-47826532 Msa1329300:three_prime_UTR 40.0%
! GTCTTGCTTTGCAACTTTTG+GGG + chr8_4:47826574-47826593 None:intergenic 40.0%
! AACTTTTCCAGCACGAGCAA+GGG + chr8_4:47826541-47826560 None:intergenic 45.0%
! TAACTTTTCCAGCACGAGCA+AGG + chr8_4:47826542-47826561 None:intergenic 45.0%
TTGCTCGTGCTGGAAAAGTT+AGG - chr8_4:47826541-47826560 Msa1329300:three_prime_UTR 45.0%
TGCTCGTGCTGGAAAAGTTA+GGG - chr8_4:47826542-47826561 Msa1329300:three_prime_UTR 45.0%
ATTGCATACGCTTGTGAGCA+CGG + chr8_4:47826617-47826636 None:intergenic 45.0%
! AACTTTTCCAGCACGAGCAA+GGG + chr8_4:47826541-47826560 None:intergenic 45.0%
! TAACTTTTCCAGCACGAGCA+AGG + chr8_4:47826542-47826561 None:intergenic 45.0%
TTGCTCGTGCTGGAAAAGTT+AGG - chr8_4:47826541-47826560 Msa1329300:three_prime_UTR 45.0%
TGCTCGTGCTGGAAAAGTTA+GGG - chr8_4:47826542-47826561 Msa1329300:three_prime_UTR 45.0%
ATTGCATACGCTTGTGAGCA+CGG + chr8_4:47826617-47826636 None:intergenic 45.0%
GCTCGTGCTGGAAAAGTTAG+GGG - chr8_4:47826543-47826562 Msa1329300:three_prime_UTR 50.0%
GCTCGTGCTGGAAAAGTTAG+GGG - chr8_4:47826543-47826562 Msa1329300:three_prime_UTR 50.0%
CGATTTGCAGCAGCAGCTCT+CGG + chr8_4:47826357-47826376 None:intergenic 55.0%
GACAAGAAGAAGCAGCCACG+AGG - chr8_4:47826591-47826610 Msa1329300:three_prime_UTR 55.0%
AGTTACCGGCGGTGACGAAA+CGG + chr8_4:47826647-47826666 None:intergenic 55.0%
GTGGGATTCGGAAAGAAGCG+TGG - chr8_4:47826793-47826812 Msa1329300:intron 55.0%
CGATTTGCAGCAGCAGCTCT+CGG + chr8_4:47826357-47826376 None:intergenic 55.0%
GACAAGAAGAAGCAGCCACG+AGG - chr8_4:47826591-47826610 Msa1329300:three_prime_UTR 55.0%
AGTTACCGGCGGTGACGAAA+CGG + chr8_4:47826647-47826666 None:intergenic 55.0%
GTGGGATTCGGAAAGAAGCG+TGG - chr8_4:47826793-47826812 Msa1329300:intron 55.0%
CATGGTTCCCTTGCTCGTGC+TGG - chr8_4:47826531-47826550 Msa1329300:three_prime_UTR 60.0%
AATCGCCGTTTCGTCACCGC+CGG - chr8_4:47826639-47826658 Msa1329300:exon 60.0%
CATGGTTCCCTTGCTCGTGC+TGG - chr8_4:47826531-47826550 Msa1329300:three_prime_UTR 60.0%
AATCGCCGTTTCGTCACCGC+CGG - chr8_4:47826639-47826658 Msa1329300:exon 60.0%
CGCTTGTGAGCACGGCCTCG+TGG + chr8_4:47826609-47826628 None:intergenic 70.0%
CGCTTGTGAGCACGGCCTCG+TGG + chr8_4:47826609-47826628 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 47826289 47827203 47826289 ID=Msa1329300;Name=Msa1329300
chr8_4 mRNA 47826289 47827203 47826289 ID=Msa1329300-mRNA-1;Parent=Msa1329300;Name=Msa1329300-mRNA-1;_AED=0.01;_eAED=0.01;_QI=130|1|1|1|1|1|3|366|62
chr8_4 exon 47826289 47826701 47826289 ID=Msa1329300-mRNA-1:exon:13702;Parent=Msa1329300-mRNA-1
chr8_4 exon 47826832 47826969 47826832 ID=Msa1329300-mRNA-1:exon:13701;Parent=Msa1329300-mRNA-1
chr8_4 exon 47827070 47827203 47827070 ID=Msa1329300-mRNA-1:exon:13700;Parent=Msa1329300-mRNA-1
chr8_4 five_prime_UTR 47827074 47827203 47827074 ID=Msa1329300-mRNA-1:five_prime_utr;Parent=Msa1329300-mRNA-1
chr8_4 CDS 47827070 47827073 47827070 ID=Msa1329300-mRNA-1:cds;Parent=Msa1329300-mRNA-1
chr8_4 CDS 47826832 47826969 47826832 ID=Msa1329300-mRNA-1:cds;Parent=Msa1329300-mRNA-1
chr8_4 CDS 47826655 47826701 47826655 ID=Msa1329300-mRNA-1:cds;Parent=Msa1329300-mRNA-1
chr8_4 three_prime_UTR 47826289 47826654 47826289 ID=Msa1329300-mRNA-1:three_prime_utr;Parent=Msa1329300-mRNA-1
Gene Sequence

>Msa1329300

ATGGGTAAAGTGCATGGTTCCCTTGCTCGTGCTGGAAAAGTTAGGGGACAAACCCCAAAAGTTGCAAAGCAAGACAAGAAGAAGCAGCCACGAGGCCGTGCTCACAAGCGTATGCAATACAATCGCCGTTTCGTCACCGCCGTTGTGGGATTCGGAAAGAAGCGTGGACCCAACTCATCTGAAAAGTGA

Protein sequence

>Msa1329300

MGKVHGSLARAGKVRGQTPKVAKQDKKKQPRGRAHKRMQYNRRFVTAVVGFGKKRGPNSSEK*