AlfalfaGEDB | Msa0715140

M. sativa cultivar zhongmu-4 / Msa0715140

KEGG: K13448
GO: GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
PFAM: PF00036.35

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0715140	XP_003611759.1	89.947	189	14	2	1	188	1	185	6.34e-114	332

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0715140	sp\|Q9ZQE6\|CML30_ARATH	41.117	197	99	4	1	190	1	187	1.96e-40	138

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0715140	G7K7Z2	89.947	189	14	2	1	188	1	185	3.03e-114	332

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000490	Msa0715140	-0.801435	9.017375e-49	-8.615850e-47
Msa0043730	Msa0715140	0.808202	3.477073e-50	-8.615850e-47
Msa0050730	Msa0715140	0.807472	4.972503e-50	-8.615850e-47
Msa0050760	Msa0715140	0.803692	3.088018e-49	-8.615850e-47
Msa0090030	Msa0715140	0.815430	9.284402e-52	-8.615850e-47
Msa0120340	Msa0715140	0.823654	1.233070e-53	-8.615850e-47
Msa0621240	Msa0715140	0.801225	9.955796e-49	-8.615850e-47
Msa0621650	Msa0715140	0.821020	5.042007e-53	-8.615850e-47
Msa0679550	Msa0715140	0.821764	3.395157e-53	-8.615850e-47
Msa0694250	Msa0715140	0.801189	1.012640e-48	-8.615850e-47
Msa0714960	Msa0715140	0.851295	9.740431e-61	-8.615850e-47
Msa0715110	Msa0715140	0.884886	1.306653e-71	-8.615850e-47
Msa0715120	Msa0715140	0.837693	4.470711e-57	-8.615850e-47
Msa0715140	Msa0715150	0.970062	4.749825e-131	-8.615850e-47
Msa0715140	Msa0735160	0.805086	1.582192e-49	-8.615850e-47
Msa0715140	Msa0757050	0.867420	1.387279e-65	-8.615850e-47
Msa0715140	Msa0757080	0.834107	3.628262e-56	-8.615850e-47
Msa0715140	Msa0771580	0.801874	7.327290e-49	-8.615850e-47
Msa0715140	Msa0798030	0.858243	9.419485e-63	-8.615850e-47
Msa0715140	Msa0798060	0.861057	1.340088e-63	-8.615850e-47
Msa0715140	Msa0798070	0.882809	7.626662e-71	-8.615850e-47
Msa0715140	Msa0820530	-0.800898	1.161377e-48	-8.615850e-47
Msa0715140	Msa0836320	0.814866	1.238890e-51	-8.615850e-47
Msa0715140	Msa0836620	0.861979	7.009900e-64	-8.615850e-47
Msa0715140	Msa0836630	0.847074	1.454968e-59	-8.615850e-47
Msa0715140	Msa0908170	0.861370	1.076056e-63	-8.615850e-47
Msa0715140	Msa0920550	0.809358	1.968586e-50	-8.615850e-47
Msa0715140	Msa0952020	0.809651	1.702824e-50	-8.615850e-47
Msa0715140	Msa0959010	0.822765	1.988135e-53	-8.615850e-47
Msa0715140	Msa0960160	0.811501	6.782784e-51	-8.615850e-47
Msa0715140	Msa0993530	0.825328	4.978220e-54	-8.615850e-47
Msa0715140	Msa1087470	0.800514	1.390913e-48	-8.615850e-47
Msa0715140	Msa1109820	0.805372	1.378170e-49	-8.615850e-47
Msa0715140	Msa1113590	0.804340	2.264211e-49	-8.615850e-47
Msa0715140	Msa1128330	0.813654	2.293956e-51	-8.615850e-47
Msa0715140	Msa1130210	-0.800404	1.464233e-48	-8.615850e-47
Msa0715140	Msa1178050	0.806019	1.007998e-49	-8.615850e-47
Msa0715140	Msa1185540	0.810818	9.542700e-51	-8.615850e-47
Msa0715140	Msa1219430	0.800836	1.195642e-48	-8.615850e-47
Msa0715140	Msa1221900	0.815801	7.675687e-52	-8.615850e-47
Msa0715140	Msa1233500	0.809836	1.553600e-50	-8.615850e-47
Msa0715140	Msa1236070	-0.808940	2.418731e-50	-8.615850e-47
Msa0715140	Msa1267840	0.826825	2.193723e-54	-8.615850e-47
Msa0715140	Msa1327760	0.802351	5.847432e-49	-8.615850e-47
Msa0715140	Msa1330890	-0.816729	4.757924e-52	-8.615850e-47
Msa0715140	Msa1337730	0.824506	7.781036e-54	-8.615850e-47
Msa0715140	Msa1338250	0.821295	4.355717e-53	-8.615850e-47
Msa0715140	Msa1340810	0.803979	2.691449e-49	-8.615850e-47
Msa0715140	Msa1363700	0.808742	2.666974e-50	-8.615850e-47
Msa0715140	Msa1379850	0.800777	1.229416e-48	-8.615850e-47
Msa0715140	Msa1390230	0.807664	4.527010e-50	-8.615850e-47
Msa0715140	Msa1393800	0.807738	4.364118e-50	-8.615850e-47
Msa0715140	Msa1415940	0.801557	8.511329e-49	-8.615850e-47
Msa0715140	Msa1422840	0.830703	2.528750e-55	-8.615850e-47
Msa0715140	Msa1433220	0.802762	4.810508e-49	-8.615850e-47
Msa0715140	Msa1440600	0.805237	1.471123e-49	-8.615850e-47
Msa0165300	Msa0715140	0.806091	9.734944e-50	-8.615850e-47
Msa0165970	Msa0715140	0.807807	4.220706e-50	-8.615850e-47
Msa0185590	Msa0715140	0.813081	3.065967e-51	-8.615850e-47
Msa0377530	Msa0715140	0.819742	9.901656e-53	-8.615850e-47
Msa0388030	Msa0715140	-0.809024	2.320938e-50	-8.615850e-47
Msa0410300	Msa0715140	0.801189	1.012732e-48	-8.615850e-47
Msa0415810	Msa0715140	0.811740	6.019837e-51	-8.615850e-47
Msa0424990	Msa0715140	0.802990	4.316289e-49	-8.615850e-47
Msa0426330	Msa0715140	0.838660	2.519676e-57	-8.615850e-47
Msa0461590	Msa0715140	0.809437	1.892997e-50	-8.615850e-47
Msa0464900	Msa0715140	0.829487	5.006997e-55	-8.615850e-47
Msa0274350	Msa0715140	0.804224	2.393230e-49	-8.615850e-47
Msa0276920	Msa0715140	0.807403	5.142832e-50	-8.615850e-47
Msa0288320	Msa0715140	0.805425	1.343481e-49	-8.615850e-47
Msa0304840	Msa0715140	0.804426	2.172362e-49	-8.615850e-47
Msa0505060	Msa0715140	0.814783	1.292067e-51	-8.615850e-47
Msa0506940	Msa0715140	0.808680	2.749324e-50	-8.615850e-47
Msa0517490	Msa0715140	0.821538	3.829122e-53	-8.615850e-47
Msa0534410	Msa0715140	0.807592	4.688484e-50	-8.615850e-47
Msa0573040	Msa0715140	0.816884	4.392729e-52	-8.615850e-47
Msa0577220	Msa0715140	0.816653	4.950107e-52	-8.615850e-47
Msa0587190	Msa0715140	0.807771	4.294185e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0715140	MtrunA17_Chr5g0402361	89.947	189	14	2	1	188	1	185	3.04e-115	332
Msa0715140	MtrunA17_Chr5g0402361	87.500	176	16	3	15	188	187	358	7.84e-101	295
Msa0715140	MtrunA17_Chr5g0402331	88.421	190	16	3	1	188	1	186	3.35e-114	323
Msa0715140	MtrunA17_Chr4g0044961	44.041	193	95	3	1	184	1	189	1.03e-44	147
Msa0715140	MtrunA17_Chr5g0402351	80.899	89	13	1	32	120	7	91	6.15e-43	139
Msa0715140	MtrunA17_Chr1g0161211	37.778	180	100	5	8	185	12	181	2.51e-31	112
Msa0715140	MtrunA17_Chr3g0111371	39.568	139	78	2	46	184	3	135	4.86e-29	105
Msa0715140	MtrunA17_Chr8g0391691	41.912	136	71	3	48	180	36	166	9.30e-29	105
Msa0715140	MtrunA17_Chr8g0392951	37.762	143	84	2	47	188	23	161	3.91e-26	98.2
Msa0715140	MtrunA17_Chr5g0434951	39.568	139	79	2	47	184	3	137	1.39e-25	96.3
Msa0715140	MtrunA17_Chr1g0169581	34.507	142	85	2	47	184	3	140	2.03e-22	88.2
Msa0715140	MtrunA17_Chr7g0245911	35.000	180	100	6	20	189	23	195	5.82e-22	88.2
Msa0715140	MtrunA17_Chr6g0482731	34.286	140	86	3	47	184	25	160	1.39e-21	86.7
Msa0715140	MtrunA17_Chr8g0351681	37.241	145	81	3	48	184	49	191	2.73e-21	86.7
Msa0715140	MtrunA17_Chr2g0329371	38.095	147	81	3	47	187	63	205	1.43e-20	85.1
Msa0715140	MtrunA17_Chr7g0256171	37.500	144	82	3	48	187	5	144	4.62e-20	82.4
Msa0715140	MtrunA17_Chr4g0033601	34.483	145	87	3	47	187	4	144	8.78e-20	81.6
Msa0715140	MtrunA17_Chr1g0188171	35.252	139	84	3	50	186	14	148	6.75e-19	79.3
Msa0715140	MtrunA17_Chr1g0169621	31.548	168	80	3	47	184	3	165	7.06e-19	79.7
Msa0715140	MtrunA17_Chr3g0121191	35.211	142	76	3	48	187	93	220	6.62e-18	78.6
Msa0715140	MtrunA17_Chr3g0116141	33.577	137	84	3	50	184	7	138	1.66e-17	75.1
Msa0715140	MtrunA17_Chr3g0116151	33.577	137	84	3	50	184	8	139	1.85e-17	75.1
Msa0715140	MtrunA17_Chr7g0225921	33.103	145	87	4	45	185	3	141	3.06e-17	74.7
Msa0715140	MtrunA17_Chr7g0256701	33.333	144	85	4	48	184	73	212	3.52e-17	76.3
Msa0715140	MtrunA17_Chr1g0166281	34.965	143	81	4	48	185	12	147	4.28e-17	74.3
Msa0715140	MtrunA17_Chr1g0185001	31.757	148	97	3	40	185	1	146	5.98e-17	73.9
Msa0715140	MtrunA17_Chr6g0459231	32.353	136	85	3	52	185	12	142	7.22e-17	73.6
Msa0715140	MtrunA17_Chr3g0093271	28.877	187	121	4	1	185	22	198	7.47e-17	75.1
Msa0715140	MtrunA17_Chr4g0064231	33.508	191	99	6	17	187	37	219	3.69e-16	73.6
Msa0715140	MtrunA17_Chr8g0366401	28.571	147	86	3	48	184	17	154	1.12e-15	71.2
Msa0715140	MtrunA17_Chr7g0254731	32.867	143	84	4	48	185	12	147	1.59e-15	70.5
Msa0715140	MtrunA17_Chr5g0440931	32.168	143	85	3	48	185	12	147	6.28e-15	68.6
Msa0715140	MtrunA17_Chr6g0460211	32.168	143	85	3	48	185	12	147	6.28e-15	68.6
Msa0715140	MtrunA17_Chr7g0245231	32.704	159	99	5	30	186	13	165	7.09e-15	68.9
Msa0715140	MtrunA17_Chr6g0450921	32.704	159	99	5	30	186	12	164	7.96e-15	68.9
Msa0715140	MtrunA17_Chr4g0057661	34.483	145	79	6	48	186	6	140	8.98e-15	68.2
Msa0715140	MtrunA17_Chr4g0057641	31.655	139	88	3	48	184	6	139	2.67e-14	67.0
Msa0715140	MtrunA17_Chr8g0373691	28.662	157	100	5	36	184	35	187	5.07e-14	67.4
Msa0715140	MtrunA17_Chr7g0274101	34.559	136	83	2	53	186	17	148	6.28e-14	66.2
Msa0715140	MtrunA17_Chr5g0396261	35.714	98	57	2	91	183	16	112	1.44e-13	64.3
Msa0715140	MtrunA17_Chr4g0057651	34.483	145	79	6	48	186	6	140	1.64e-13	64.7
Msa0715140	MtrunA17_Chr4g0062221	29.655	145	88	4	47	184	10	147	3.36e-13	64.7
Msa0715140	MtrunA17_Chr8g0368681	30.519	154	92	5	40	184	1	148	3.55e-13	64.3
Msa0715140	MtrunA17_Chr3g0125311	28.662	157	84	6	48	185	13	160	1.34e-12	62.8
Msa0715140	MtrunA17_Chr7g0267751	30.714	140	90	3	49	187	441	574	3.36e-12	64.3
Msa0715140	MtrunA17_Chr1g0170281	31.034	145	90	5	49	191	424	560	2.25e-11	62.0
Msa0715140	MtrunA17_Chr1g0176751	29.078	141	91	4	49	187	430	563	5.99e-11	60.8
Msa0715140	MtrunA17_Chr1g0157131	28.169	142	92	4	49	187	380	514	6.32e-11	60.5
Msa0715140	MtrunA17_Chr5g0444171	28.571	140	94	3	49	187	398	532	7.63e-11	60.5
Msa0715140	MtrunA17_Chr5g0409441	28.846	156	94	5	37	185	11	156	8.78e-11	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0715140	AT2G15680.1	41.117	197	99	4	1	190	1	187	2.00e-41	138
Msa0715140	AT1G24620.1	41.259	143	76	3	48	187	37	174	1.70e-30	110
Msa0715140	AT5G37770.1	40.136	147	78	2	47	187	16	158	7.18e-30	108
Msa0715140	AT5G37770.2	40.136	147	78	2	47	187	16	158	7.18e-30	108
Msa0715140	AT1G66400.1	39.583	144	77	3	47	185	14	152	3.47e-28	103
Msa0715140	AT1G18210.2	36.025	161	95	3	24	184	1	153	8.86e-28	103
Msa0715140	AT1G18210.1	36.025	161	95	3	24	184	1	153	8.86e-28	103
Msa0715140	AT3G03400.1	40.625	128	68	2	52	175	9	132	3.89e-27	100
Msa0715140	AT5G17470.1	42.336	137	71	3	52	184	6	138	8.99e-27	99.8
Msa0715140	AT5G42380.1	36.879	141	82	3	47	184	48	184	2.10e-25	97.4
Msa0715140	AT1G73630.1	36.913	149	87	3	37	184	8	150	4.82e-25	95.9
Msa0715140	AT2G36180.1	41.259	143	74	4	49	186	1	138	4.74e-23	90.1
Msa0715140	AT1G05990.1	39.310	145	79	3	48	187	5	145	2.59e-21	85.9
Msa0715140	AT2G43290.1	35.570	149	83	3	48	187	65	209	7.45e-21	86.3
Msa0715140	AT3G59440.1	34.932	146	82	4	48	187	51	189	2.20e-20	84.7
Msa0715140	AT4G14640.1	36.879	141	83	3	48	186	13	149	2.29e-20	83.6
Msa0715140	AT3G50360.1	37.324	142	83	3	45	184	24	161	4.39e-20	83.2
Msa0715140	AT4G14640.2	35.772	123	77	1	66	186	9	131	9.22e-20	81.3
Msa0715140	AT3G50770.1	32.022	178	101	6	14	184	39	203	1.11e-19	82.8
Msa0715140	AT1G76650.1	30.899	178	111	4	14	184	4	176	1.49e-19	82.0
Msa0715140	AT1G76650.3	30.899	178	111	4	14	184	4	176	1.49e-19	82.0
Msa0715140	AT1G76640.1	33.758	157	95	3	33	184	6	158	1.10e-18	79.3
Msa0715140	AT4G03290.1	35.811	148	84	3	48	188	5	148	4.71e-18	77.4
Msa0715140	AT3G22930.1	36.364	143	84	4	48	187	35	173	7.52e-18	77.4
Msa0715140	AT3G07490.1	34.722	144	86	3	48	187	5	144	2.22e-17	75.5
Msa0715140	AT3G10190.1	41.489	94	53	2	93	184	112	205	1.35e-16	74.7
Msa0715140	AT2G41410.1	42.857	77	43	1	114	189	136	212	2.53e-16	74.3
Msa0715140	AT1G32250.1	27.333	150	89	3	47	184	15	156	4.58e-16	72.4
Msa0715140	AT3G03410.1	34.559	136	78	2	50	185	4	128	1.09e-15	70.5
Msa0715140	AT2G41110.2	30.405	148	93	2	48	185	12	159	1.95e-15	70.9
Msa0715140	AT1G66410.1	31.429	140	90	2	48	185	12	147	3.78e-15	69.7
Msa0715140	AT5G37780.1	31.429	140	90	2	48	185	12	147	3.78e-15	69.7
Msa0715140	AT5G21274.1	32.867	143	84	3	48	185	12	147	4.03e-15	69.7
Msa0715140	AT3G56800.1	32.143	140	89	2	48	185	12	147	5.13e-15	69.3
Msa0715140	AT2G41110.1	32.143	140	89	2	48	185	12	147	5.13e-15	69.3
Msa0715140	AT2G27030.1	32.143	140	89	2	48	185	12	147	5.13e-15	69.3
Msa0715140	AT4G12860.1	34.247	146	88	3	46	187	3	144	5.14e-15	69.3
Msa0715140	AT2G27030.3	33.566	143	83	4	48	185	12	147	5.67e-15	70.1
Msa0715140	AT3G43810.2	32.168	143	85	3	48	185	12	147	6.07e-15	70.5
Msa0715140	AT3G43810.1	32.168	143	85	3	48	185	12	147	8.67e-15	68.6
Msa0715140	AT3G03000.1	30.714	140	90	3	48	184	20	155	3.98e-14	67.4
Msa0715140	AT5G37780.4	30.469	128	81	2	63	185	10	134	6.14e-14	66.2
Msa0715140	AT1G76650.2	28.090	178	106	5	14	184	4	166	7.73e-14	66.6
Msa0715140	AT5G37780.3	30.081	123	84	1	65	185	51	173	7.95e-14	66.6
Msa0715140	AT1G66410.4	37.895	95	51	2	96	185	20	111	8.26e-14	65.1
Msa0715140	AT1G66410.3	37.895	95	51	2	96	185	20	111	8.26e-14	65.1
Msa0715140	AT1G66410.2	30.328	122	83	1	66	185	36	157	1.13e-13	65.9
Msa0715140	AT5G37780.2	28.477	151	95	2	48	185	12	162	1.15e-13	66.2
Msa0715140	AT2G41100.7	30.247	162	89	4	48	190	66	222	1.44e-13	67.8
Msa0715140	AT2G41100.6	30.247	162	89	4	48	190	66	222	1.44e-13	67.8
Msa0715140	AT2G41100.3	30.247	162	89	4	48	190	66	222	1.44e-13	67.8
Msa0715140	AT3G43810.4	37.895	95	51	2	96	185	20	111	1.82e-13	64.3
Msa0715140	AT3G43810.3	37.895	95	51	2	96	185	20	111	1.82e-13	64.3
Msa0715140	AT2G41100.4	30.247	162	89	4	48	190	101	257	2.07e-13	67.8
Msa0715140	AT2G41100.1	30.247	162	89	4	48	190	101	257	2.07e-13	67.8
Msa0715140	AT4G37010.1	30.070	143	92	3	45	184	24	161	3.86e-13	64.7
Msa0715140	AT4G37010.2	30.070	143	92	3	45	184	28	165	3.95e-13	64.7
Msa0715140	AT5G04870.1	32.394	142	85	5	49	187	456	589	7.41e-13	66.6
Msa0715140	AT5G44460.1	35.227	176	92	9	32	191	11	180	1.16e-12	63.9
Msa0715140	AT2G17890.1	31.429	140	78	4	53	184	420	549	1.96e-12	65.5
Msa0715140	AT1G18530.1	29.577	142	90	4	48	184	7	143	2.96e-12	62.0
Msa0715140	AT4G04720.2	30.405	148	91	4	49	192	385	524	3.15e-12	64.7
Msa0715140	AT4G04720.1	30.405	148	91	4	49	192	385	524	3.15e-12	64.7
Msa0715140	AT2G17290.1	31.429	140	89	3	49	187	391	524	4.64e-12	64.3
Msa0715140	AT2G17290.2	31.429	140	89	3	49	187	391	524	4.64e-12	64.3
Msa0715140	AT1G21550.1	46.269	67	34	1	122	186	88	154	5.03e-12	61.6
Msa0715140	AT4G36070.1	30.769	156	86	6	47	190	377	522	5.48e-12	63.9
Msa0715140	AT4G04740.4	28.966	145	97	3	49	192	374	513	6.27e-12	63.9
Msa0715140	AT4G04740.6	28.966	145	97	3	49	192	374	513	6.27e-12	63.9
Msa0715140	AT4G04740.5	28.966	145	97	3	49	192	374	513	6.27e-12	63.9
Msa0715140	AT4G04740.3	28.966	145	97	3	49	192	374	513	6.27e-12	63.9
Msa0715140	AT4G04740.1	28.966	145	97	3	49	192	374	513	6.27e-12	63.9
Msa0715140	AT4G04740.7	28.966	145	97	3	49	192	374	513	6.27e-12	63.9
Msa0715140	AT5G12480.2	32.624	141	89	3	49	187	271	407	6.56e-12	63.9
Msa0715140	AT4G36070.2	30.769	156	86	6	47	190	377	522	6.98e-12	63.9
Msa0715140	AT3G10660.2	31.206	141	88	4	49	187	492	625	9.08e-12	63.5
Msa0715140	AT3G10660.1	31.206	141	88	4	49	187	492	625	9.08e-12	63.5
Msa0715140	AT5G12480.1	32.624	141	89	3	49	187	365	501	9.60e-12	63.5
Msa0715140	AT2G41100.2	32.456	114	57	3	96	190	20	132	1.67e-11	60.8
Msa0715140	AT3G25600.1	28.472	144	89	4	48	184	12	148	2.00e-11	60.1
Msa0715140	AT4G04695.1	30.070	143	94	3	49	190	337	474	3.14e-11	62.0
Msa0715140	AT4G38230.1	31.429	140	89	3	49	187	186	319	5.09e-11	60.8
Msa0715140	AT4G38230.2	31.429	140	89	3	49	187	360	493	5.61e-11	61.2
Msa0715140	AT4G38230.4	31.429	140	89	3	49	187	330	463	6.05e-11	60.8
Msa0715140	AT4G38230.3	31.429	140	89	3	49	187	330	463	6.05e-11	60.8
Msa0715140	AT2G41090.1	28.986	138	93	2	48	185	12	144	6.54e-11	59.3
Msa0715140	AT5G23580.1	29.197	137	90	3	49	184	328	458	7.00e-11	60.8

Find 34 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTCAGAGTCCATTGCTTTAA+AGG	0.108453	5_1:-8047022	None:intergenic
CATTTCATTTCATCTTTCTT+TGG	0.177414	5_1:-8046680	None:intergenic
TTTCAAGTATTTGATATAAA+TGG	0.216373	5_1:+8047129	Msa0715140:CDS
CATAGACTTTAAAGAGTTCA+TGG	0.277650	5_1:+8047056	Msa0715140:CDS
TTGAGTTTCCTTAAGTTTCA+TGG	0.307474	5_1:+8046560	Msa0715140:CDS
CCTTAGTGCTTGTAAGAAAA+TGG	0.341545	5_1:+8047215	Msa0715140:CDS
CAAAGAAAGATGAAATGAAA+TGG	0.359646	5_1:+8046681	Msa0715140:CDS
CTTTGGTTGGAAGCTCATTT+CGG	0.372802	5_1:-8046663	None:intergenic
AATTGTCTCAAATATTTAAG+AGG	0.387521	5_1:+8047175	Msa0715140:CDS
GCTGCAAAAGCTTTGGATAA+GGG	0.402200	5_1:+8046973	Msa0715140:CDS
TAAAGCAGCTGCAAAAGCTT+TGG	0.411228	5_1:+8046966	Msa0715140:CDS
TCATTTCATCTTTCTTTGGT+TGG	0.420505	5_1:-8046676	None:intergenic
TCTCAAATATTTAAGAGGTT+AGG	0.421604	5_1:+8047180	Msa0715140:CDS
AAAAGCTACCATGAAACTTA+AGG	0.427001	5_1:-8046568	None:intergenic
TAAAGATGGCAAGATTAGTC+TGG	0.431211	5_1:+8046936	Msa0715140:intron
AGCTGCAAAAGCTTTGGATA+AGG	0.434736	5_1:+8046972	Msa0715140:CDS
AAAGAAAGATGAAATGAAAT+GGG	0.477872	5_1:+8046682	Msa0715140:CDS
AAGCTTTGGATAAGGGGATA+GGG	0.522510	5_1:+8046980	Msa0715140:CDS
CTGCAAAAGCTTTGGATAAG+GGG	0.525087	5_1:+8046974	Msa0715140:CDS
AAAGCTTTGGATAAGGGGAT+AGG	0.538133	5_1:+8046979	Msa0715140:CDS
GTATTTGATATAAATGGTGA+TGG	0.547891	5_1:+8047135	Msa0715140:CDS
GAGTTCATGGAAATGTTCAA+TGG	0.548455	5_1:+8047069	Msa0715140:CDS
GTGAAAGGTGTTGATAGTGA+TGG	0.562762	5_1:+8047237	Msa0715140:CDS
TAGATTTGAATGAGTTTGCA+AGG	0.576417	5_1:+8047271	Msa0715140:CDS
AAATTTGACACAAACAAAGA+TGG	0.579839	5_1:+8046713	Msa0715140:CDS
AGCTTTGGATAAGGGGATAG+GGG	0.581892	5_1:+8046981	Msa0715140:CDS
ATGGAAATGTTCAATGGAGA+GGG	0.592453	5_1:+8047075	Msa0715140:CDS
CATGGAAATGTTCAATGGAG+AGG	0.594726	5_1:+8047074	Msa0715140:CDS
TATTTGATATAAATGGTGAT+GGG	0.605312	5_1:+8047136	Msa0715140:CDS
AGTGAAAGCCTTTAAAGCAA+TGG	0.615356	5_1:+8047014	Msa0715140:CDS
GCAATGGACTCTGACAAAGA+TGG	0.615789	5_1:+8047030	Msa0715140:CDS
GGTGTTGATAGTGATGGAGA+TGG	0.629261	5_1:+8047243	Msa0715140:CDS
TTTGCAAGGATGATAATGAA+TGG	0.633397	5_1:+8047285	Msa0715140:CDS
GCTTGTAAGAAAATGGTGAA+AGG	0.660596	5_1:+8047222	Msa0715140:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTCAAGTATTTGATATAAA+TGG	+	chr5_1:8047129-8047148	Msa0715140:CDS	15.0%
!!!	TTTCAAGTATTTGATATAAA+TGG	+	chr5_1:8047129-8047148	Msa0715140:CDS	15.0%
!!	AAAGAAAGATGAAATGAAAT+GGG	+	chr5_1:8046682-8046701	Msa0715140:CDS	20.0%
!!	AAAGAAAGATGAAATGAAAT+GGG	+	chr5_1:8046682-8046701	Msa0715140:CDS	20.0%
!!	TATTTGATATAAATGGTGAT+GGG	+	chr5_1:8047136-8047155	Msa0715140:CDS	20.0%
!!	AATTGTCTCAAATATTTAAG+AGG	+	chr5_1:8047175-8047194	Msa0715140:CDS	20.0%
!!	AAAGAAAGATGAAATGAAAT+GGG	+	chr5_1:8046682-8046701	Msa0715140:CDS	20.0%
!!	AAAGAAAGATGAAATGAAAT+GGG	+	chr5_1:8046682-8046701	Msa0715140:CDS	20.0%
!!	TATTTGATATAAATGGTGAT+GGG	+	chr5_1:8047136-8047155	Msa0715140:CDS	20.0%
!!	AATTGTCTCAAATATTTAAG+AGG	+	chr5_1:8047175-8047194	Msa0715140:CDS	20.0%
!!!	TTTTGAGCTATATTTTTTCG+TGG	-	chr5_1:8046611-8046630	None:intergenic	25.0%
!	CATTTCATTTCATCTTTCTT+TGG	-	chr5_1:8046683-8046702	None:intergenic	25.0%
!	CAAAGAAAGATGAAATGAAA+TGG	+	chr5_1:8046681-8046700	Msa0715140:CDS	25.0%
!	AAATTTGACACAAACAAAGA+TGG	+	chr5_1:8046713-8046732	Msa0715140:CDS	25.0%
!	CATTTCATTTCATCTTTCTT+TGG	-	chr5_1:8046683-8046702	None:intergenic	25.0%
!	CAAAGAAAGATGAAATGAAA+TGG	+	chr5_1:8046681-8046700	Msa0715140:CDS	25.0%
!	AAATTCGACACTAATAAAGA+TGG	+	chr5_1:8046922-8046941	Msa0715140:intron	25.0%
!	GTATTTGATATAAATGGTGA+TGG	+	chr5_1:8047135-8047154	Msa0715140:CDS	25.0%
!	TCTCAAATATTTAAGAGGTT+AGG	+	chr5_1:8047180-8047199	Msa0715140:CDS	25.0%
!!!	TTTTGAGCTATATTTTTTCG+TGG	-	chr5_1:8046611-8046630	None:intergenic	25.0%
!	CATTTCATTTCATCTTTCTT+TGG	-	chr5_1:8046683-8046702	None:intergenic	25.0%
!	CAAAGAAAGATGAAATGAAA+TGG	+	chr5_1:8046681-8046700	Msa0715140:CDS	25.0%
!	AAATTTGACACAAACAAAGA+TGG	+	chr5_1:8046713-8046732	Msa0715140:CDS	25.0%
!	CATTTCATTTCATCTTTCTT+TGG	-	chr5_1:8046683-8046702	None:intergenic	25.0%
!	CAAAGAAAGATGAAATGAAA+TGG	+	chr5_1:8046681-8046700	Msa0715140:CDS	25.0%
!	AAATTCGACACTAATAAAGA+TGG	+	chr5_1:8046922-8046941	Msa0715140:intron	25.0%
!	GTATTTGATATAAATGGTGA+TGG	+	chr5_1:8047135-8047154	Msa0715140:CDS	25.0%
!	TCTCAAATATTTAAGAGGTT+AGG	+	chr5_1:8047180-8047199	Msa0715140:CDS	25.0%
	TTGAGTTTCCTTAAGTTTCA+TGG	+	chr5_1:8046560-8046579	Msa0715140:CDS	30.0%
	AAAAGCTACCATGAAACTTA+AGG	-	chr5_1:8046571-8046590	None:intergenic	30.0%
!!!	ATATTTTTTCGTGGAGATGT+TGG	-	chr5_1:8046602-8046621	None:intergenic	30.0%
	TCATTTCATCTTTCTTTGGT+TGG	-	chr5_1:8046679-8046698	None:intergenic	30.0%
	TCATTTCATCTTTCTTTGGT+TGG	-	chr5_1:8046679-8046698	None:intergenic	30.0%
!	CATAGACTTTAAAGAGTTCA+TGG	+	chr5_1:8047056-8047075	Msa0715140:CDS	30.0%
	TAGATTTGAATGAGTTTGCA+AGG	+	chr5_1:8047271-8047290	Msa0715140:CDS	30.0%
!	TTTGCAAGGATGATAATGAA+TGG	+	chr5_1:8047285-8047304	Msa0715140:CDS	30.0%
	TTGAGTTTCCTTAAGTTTCA+TGG	+	chr5_1:8046560-8046579	Msa0715140:CDS	30.0%
	AAAAGCTACCATGAAACTTA+AGG	-	chr5_1:8046571-8046590	None:intergenic	30.0%
!!!	ATATTTTTTCGTGGAGATGT+TGG	-	chr5_1:8046602-8046621	None:intergenic	30.0%
	TCATTTCATCTTTCTTTGGT+TGG	-	chr5_1:8046679-8046698	None:intergenic	30.0%
	TCATTTCATCTTTCTTTGGT+TGG	-	chr5_1:8046679-8046698	None:intergenic	30.0%
!	CATAGACTTTAAAGAGTTCA+TGG	+	chr5_1:8047056-8047075	Msa0715140:CDS	30.0%
	TAGATTTGAATGAGTTTGCA+AGG	+	chr5_1:8047271-8047290	Msa0715140:CDS	30.0%
!	TTTGCAAGGATGATAATGAA+TGG	+	chr5_1:8047285-8047304	Msa0715140:CDS	30.0%
!	TAAAGATGGCAAGATTAGTC+TGG	+	chr5_1:8046936-8046955	Msa0715140:intron	35.0%
!!	AGTGAAAGCCTTTAAAGCAA+TGG	+	chr5_1:8047014-8047033	Msa0715140:CDS	35.0%
	GAGTTCATGGAAATGTTCAA+TGG	+	chr5_1:8047069-8047088	Msa0715140:CDS	35.0%
	ATGGAAATGTTCAATGGAGA+GGG	+	chr5_1:8047075-8047094	Msa0715140:CDS	35.0%
!	CCATTTTCTTACAAGCACTA+AGG	-	chr5_1:8047218-8047237	None:intergenic	35.0%
	CCTTAGTGCTTGTAAGAAAA+TGG	+	chr5_1:8047215-8047234	Msa0715140:CDS	35.0%
	GCTTGTAAGAAAATGGTGAA+AGG	+	chr5_1:8047222-8047241	Msa0715140:CDS	35.0%
!	TAAAGATGGCAAGATTAGTC+TGG	+	chr5_1:8046936-8046955	Msa0715140:intron	35.0%
!!	AGTGAAAGCCTTTAAAGCAA+TGG	+	chr5_1:8047014-8047033	Msa0715140:CDS	35.0%
	GAGTTCATGGAAATGTTCAA+TGG	+	chr5_1:8047069-8047088	Msa0715140:CDS	35.0%
	ATGGAAATGTTCAATGGAGA+GGG	+	chr5_1:8047075-8047094	Msa0715140:CDS	35.0%
!	CCATTTTCTTACAAGCACTA+AGG	-	chr5_1:8047218-8047237	None:intergenic	35.0%
	CCTTAGTGCTTGTAAGAAAA+TGG	+	chr5_1:8047215-8047234	Msa0715140:CDS	35.0%
	GCTTGTAAGAAAATGGTGAA+AGG	+	chr5_1:8047222-8047241	Msa0715140:CDS	35.0%
	CTTTGGTTGGAAGCTCATTT+CGG	-	chr5_1:8046666-8046685	None:intergenic	40.0%
!	CAAAGATGGCAAGATTAGTC+TGG	+	chr5_1:8046727-8046746	Msa0715140:intron	40.0%
	TAAAGCAGCTGCAAAAGCTT+TGG	+	chr5_1:8046757-8046776	Msa0715140:intron	40.0%
!!	AGCTGCAAAAGCTTTGGATA+AGG	+	chr5_1:8046972-8046991	Msa0715140:CDS	40.0%
!!	GCTGCAAAAGCTTTGGATAA+GGG	+	chr5_1:8046973-8046992	Msa0715140:CDS	40.0%
!!	CTGCAAAAGCTTTGGATAAG+GGG	+	chr5_1:8046974-8046993	Msa0715140:CDS	40.0%
!!	AAAGCTTTGGATAAGGGGAT+AGG	+	chr5_1:8046979-8046998	Msa0715140:CDS	40.0%
!!	AAGCTTTGGATAAGGGGATA+GGG	+	chr5_1:8046980-8046999	Msa0715140:CDS	40.0%
	GTCAGAGTCCATTGCTTTAA+AGG	-	chr5_1:8047025-8047044	None:intergenic	40.0%
	CATGGAAATGTTCAATGGAG+AGG	+	chr5_1:8047074-8047093	Msa0715140:CDS	40.0%
!!	GTGAAAGGTGTTGATAGTGA+TGG	+	chr5_1:8047237-8047256	Msa0715140:CDS	40.0%
	CTTTGGTTGGAAGCTCATTT+CGG	-	chr5_1:8046666-8046685	None:intergenic	40.0%
!	CAAAGATGGCAAGATTAGTC+TGG	+	chr5_1:8046727-8046746	Msa0715140:intron	40.0%
	TAAAGCAGCTGCAAAAGCTT+TGG	+	chr5_1:8046757-8046776	Msa0715140:intron	40.0%
!!	AGCTGCAAAAGCTTTGGATA+AGG	+	chr5_1:8046972-8046991	Msa0715140:CDS	40.0%
!!	GCTGCAAAAGCTTTGGATAA+GGG	+	chr5_1:8046973-8046992	Msa0715140:CDS	40.0%
!!	CTGCAAAAGCTTTGGATAAG+GGG	+	chr5_1:8046974-8046993	Msa0715140:CDS	40.0%
!!	AAAGCTTTGGATAAGGGGAT+AGG	+	chr5_1:8046979-8046998	Msa0715140:CDS	40.0%
!!	AAGCTTTGGATAAGGGGATA+GGG	+	chr5_1:8046980-8046999	Msa0715140:CDS	40.0%
	GTCAGAGTCCATTGCTTTAA+AGG	-	chr5_1:8047025-8047044	None:intergenic	40.0%
	CATGGAAATGTTCAATGGAG+AGG	+	chr5_1:8047074-8047093	Msa0715140:CDS	40.0%
!!	GTGAAAGGTGTTGATAGTGA+TGG	+	chr5_1:8047237-8047256	Msa0715140:CDS	40.0%
!!	TTTTCGTGGAGATGTTGGCT+TGG	-	chr5_1:8046597-8046616	None:intergenic	45.0%
!!	AGCTTTGGATAAGGGGATAG+GGG	+	chr5_1:8046981-8047000	Msa0715140:CDS	45.0%
!	GCAATGGACTCTGACAAAGA+TGG	+	chr5_1:8047030-8047049	Msa0715140:CDS	45.0%
!!	GGTGTTGATAGTGATGGAGA+TGG	+	chr5_1:8047243-8047262	Msa0715140:CDS	45.0%
!!	TTTTCGTGGAGATGTTGGCT+TGG	-	chr5_1:8046597-8046616	None:intergenic	45.0%
!!	AGCTTTGGATAAGGGGATAG+GGG	+	chr5_1:8046981-8047000	Msa0715140:CDS	45.0%
!	GCAATGGACTCTGACAAAGA+TGG	+	chr5_1:8047030-8047049	Msa0715140:CDS	45.0%
!!	GGTGTTGATAGTGATGGAGA+TGG	+	chr5_1:8047243-8047262	Msa0715140:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	8046551	8047326	8046551	ID=Msa0715140;Name=Msa0715140
chr5_1	mRNA	8046551	8047326	8046551	ID=Msa0715140-mRNA-1;Parent=Msa0715140;Name=Msa0715140-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0\|0\|0\|1\|0\|0\|2\|0\|192
chr5_1	exon	8046551	8046743	8046551	ID=Msa0715140-mRNA-1:exon:3323;Parent=Msa0715140-mRNA-1
chr5_1	exon	8046941	8047326	8046941	ID=Msa0715140-mRNA-1:exon:3324;Parent=Msa0715140-mRNA-1
chr5_1	CDS	8046551	8046743	8046551	ID=Msa0715140-mRNA-1:cds;Parent=Msa0715140-mRNA-1
chr5_1	CDS	8046941	8047326	8046941	ID=Msa0715140-mRNA-1:cds;Parent=Msa0715140-mRNA-1

Gene Sequence

>Msa0715140

ATGTCAAAATTGAGTTTCCTTAAGTTTCATGGTAGCTTTTCAGCCAAGCCAACATCTCCACGAAAAAATATAGCTCAAAAGAACAGCTTCAAGTCGAGACAAACATCGAGTACCGAAATGAGCTTCCAACCAAAGAAAGATGAAATGAAATGGGTGTTCGAGAAATTTGACACAAACAAAGATGGCAAGATTAATGGCAAGATTAGTCTGGAAAAGTATAAAGCAGCTGCAAAAGCTTTGGATAAGGGGATAGGGGATACTGATGCAGTGAAAGCCTTTAAAGCAATGGACTCTGACAAAGATGGCTTCATAGACTTTAAAGAGTTCATGGAAATGTTCAATGGAGAGGGTAGTAAGATAAAAGAAGAGATTAAGAGTGCTTTTCAAGTATTTGATATAAATGGTGATGGGAAAATTAGTGCTGATGAATTGTCTCAAATATTTAAGAGGTTAGGAGAGAGTTGTAGCCTTAGTGCTTGTAAGAAAATGGTGAAAGGTGTTGATAGTGATGGAGATGGTCTTGTAGATTTGAATGAGTTTGCAAGGATGATAATGAATGGCAAGAATGTGCCTAAGTGA

Protein sequence

>Msa0715140

MSKLSFLKFHGSFSAKPTSPRKNIAQKNSFKSRQTSSTEMSFQPKKDEMKWVFEKFDTNKDGKINGKISLEKYKAAAKALDKGIGDTDAVKAFKAMDSDKDGFIDFKEFMEMFNGEGSKIKEEIKSAFQVFDINGDGKISADELSQIFKRLGESCSLSACKKMVKGVDSDGDGLVDLNEFARMIMNGKNVPK*