AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1267840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1267840 MtrunA17_Chr8g0357481 98.007 301 6 0 1 301 1 301 0.0 605
Msa1267840 MtrunA17_Chr7g0272961 30.882 272 155 10 9 264 34 288 2.09e-25 102
Msa1267840 MtrunA17_Chr4g0074511 31.698 265 160 8 14 268 40 293 7.57e-25 101
Msa1267840 MtrunA17_Chr4g0032141 29.644 253 169 4 14 265 56 300 5.51e-24 99.4
Msa1267840 MtrunA17_Chr1g0191751 30.075 266 165 7 9 264 95 349 9.11e-24 99.4
Msa1267840 MtrunA17_Chr7g0239451 28.906 256 169 4 14 264 16 263 4.06e-23 95.9
Msa1267840 MtrunA17_Chr4g0044131 32.950 261 150 9 14 264 8 253 6.07e-23 95.5
Msa1267840 MtrunA17_Chr3g0112271 29.615 260 160 8 14 265 17 261 7.07e-23 95.5
Msa1267840 MtrunA17_Chr3g0112141 27.969 261 163 9 14 265 17 261 2.05e-22 94.4
Msa1267840 MtrunA17_Chr7g0244061 29.693 293 165 9 4 269 19 297 3.27e-22 94.4
Msa1267840 MtrunA17_Chr3g0112261 29.167 264 156 8 14 265 19 263 4.21e-22 93.6
Msa1267840 MtrunA17_Chr3g0112151 27.451 255 172 4 14 265 86 330 5.34e-22 94.4
Msa1267840 MtrunA17_Chr4g0075531 30.888 259 161 5 11 265 4 248 7.56e-22 92.4
Msa1267840 MtrunA17_Chr4g0026001 31.439 264 153 9 14 267 14 259 7.59e-22 92.8
Msa1267840 MtrunA17_Chr3g0122901 29.213 267 174 6 3 267 67 320 3.39e-21 91.7
Msa1267840 MtrunA17_Chr7g0266981 30.855 269 161 10 14 272 13 266 3.80e-21 90.9
Msa1267840 MtrunA17_Chr0c30g0494101 31.461 267 156 11 14 268 40 291 5.69e-21 90.5
Msa1267840 MtrunA17_Chr3g0112171 27.165 254 174 5 14 265 15 259 9.91e-21 89.7
Msa1267840 MtrunA17_Chr7g0239481 27.481 262 171 5 14 267 15 265 1.09e-20 89.4
Msa1267840 MtrunA17_Chr7g0239511 27.574 272 182 6 1 265 1 264 1.99e-20 88.6
Msa1267840 MtrunA17_Chr4g0025971 29.670 273 164 8 14 276 14 268 2.05e-20 88.6
Msa1267840 MtrunA17_Chr3g0084871 31.047 277 163 9 3 265 7 269 3.32e-20 88.2
Msa1267840 MtrunA17_Chr4g0044111 30.112 269 163 10 14 272 13 266 4.09e-20 87.8
Msa1267840 MtrunA17_Chr7g0239491 27.237 257 173 5 14 265 15 262 4.90e-20 87.4
Msa1267840 MtrunA17_Chr4g0032181 28.926 242 153 6 30 265 17 245 5.97e-20 87.0
Msa1267840 MtrunA17_Chr4g0025981 30.620 258 165 7 15 269 42 288 1.21e-19 87.4
Msa1267840 MtrunA17_Chr7g0239501 27.798 277 175 7 1 265 1 264 2.24e-19 85.9
Msa1267840 MtrunA17_Chr7g0266891 30.627 271 163 10 12 272 5 260 2.27e-19 85.9
Msa1267840 MtrunA17_Chr7g0259521 29.851 268 166 10 14 271 6 261 2.96e-19 85.5
Msa1267840 MtrunA17_Chr8g0386201 31.839 223 136 6 9 227 40 250 3.73e-18 83.6
Msa1267840 MtrunA17_Chr7g0267001 30.888 259 140 10 14 264 8 235 4.93e-18 81.6
Msa1267840 MtrunA17_Chr4g0028921 30.000 260 164 9 14 264 11 261 4.98e-18 82.0
Msa1267840 MtrunA17_Chr1g0182511 30.882 272 170 8 1 264 1 262 1.43e-17 80.9
Msa1267840 MtrunA17_Chr4g0028911 28.897 263 163 9 14 264 11 261 1.06e-16 78.6
Msa1267840 MtrunA17_Chr7g0259531 28.625 269 174 10 4 265 2 259 1.08e-16 78.2
Msa1267840 MtrunA17_Chr7g0268591 30.827 266 155 11 14 265 33 283 1.41e-16 78.6
Msa1267840 MtrunA17_Chr7g0270451 30.855 269 167 10 14 274 6 263 2.90e-16 77.0
Msa1267840 MtrunA17_Chr7g0267011 31.980 197 120 5 14 207 8 193 3.33e-16 75.5
Msa1267840 MtrunA17_Chr2g0324021 28.464 267 173 10 14 274 6 260 3.50e-16 77.0
Msa1267840 MtrunA17_Chr7g0252031 29.699 266 159 12 14 265 35 286 1.16e-15 75.9
Msa1267840 MtrunA17_Chr4g0040371 29.474 285 162 11 1 265 5 270 2.23e-15 74.7
Msa1267840 MtrunA17_Chr8g0386171 30.493 223 123 6 12 206 50 268 4.73e-15 74.7
Msa1267840 MtrunA17_Chr7g0270441 27.132 258 172 8 14 265 6 253 1.66e-14 72.0
Msa1267840 MtrunA17_Chr7g0239461 26.923 260 121 8 14 265 16 214 8.34e-14 69.3
Msa1267840 MtrunA17_Chr1g0171831 30.516 213 125 9 6 204 5 208 4.96e-13 67.8
Msa1267840 MtrunA17_Chr3g0131521 29.932 147 96 2 15 161 33 172 2.22e-12 66.6
Msa1267840 MtrunA17_Chr1g0160511 28.571 147 98 2 15 161 38 177 3.54e-11 62.8
Msa1267840 MtrunA17_Chr8g0385581 30.000 150 95 3 15 161 35 177 6.55e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1267840 AT3G12800.1 77.778 297 65 1 6 301 2 298 3.41e-162 453
Msa1267840 AT2G07640.1 74.667 150 37 1 131 279 1 150 1.89e-69 213
Msa1267840 AT3G05260.1 31.502 273 167 7 5 268 27 288 5.44e-29 112
Msa1267840 AT1G54870.1 31.538 260 158 7 14 264 82 330 6.91e-28 110
Msa1267840 AT1G54870.2 31.538 260 158 7 14 264 84 332 7.99e-28 110
Msa1267840 AT1G63380.3 32.184 261 158 9 14 264 15 266 4.89e-27 107
Msa1267840 AT1G63380.4 32.184 261 158 9 14 264 19 270 5.23e-27 107
Msa1267840 AT1G63380.1 32.184 261 158 9 14 264 17 268 6.45e-27 107
Msa1267840 AT1G63380.2 32.184 261 158 9 14 264 22 273 6.81e-27 107
Msa1267840 AT2G17845.1 34.470 264 150 10 14 264 47 300 8.40e-27 107
Msa1267840 AT1G62610.2 31.801 261 159 9 14 264 13 264 5.66e-25 101
Msa1267840 AT1G62610.1 31.801 261 159 9 14 264 14 265 5.89e-25 101
Msa1267840 AT1G62610.3 31.801 261 159 9 14 264 15 266 5.94e-25 101
Msa1267840 AT1G52340.1 31.636 275 161 9 14 272 18 281 6.42e-25 101
Msa1267840 AT1G62610.4 31.801 261 159 9 14 264 19 270 7.16e-25 101
Msa1267840 AT1G36580.1 68.254 63 20 0 228 290 1 63 7.20e-24 96.7
Msa1267840 AT4G05530.1 33.077 260 154 6 11 265 6 250 1.13e-23 97.4
Msa1267840 AT2G29260.1 29.804 255 166 5 14 265 68 312 1.91e-23 98.2
Msa1267840 AT3G55290.1 33.333 267 146 11 14 264 18 268 5.14e-23 96.3
Msa1267840 AT3G55310.1 33.083 266 148 11 14 264 17 267 5.59e-23 96.3
Msa1267840 AT3G55290.2 33.333 267 146 11 14 264 17 267 5.77e-23 96.3
Msa1267840 AT2G29290.2 29.658 263 174 7 14 274 7 260 1.83e-22 94.4
Msa1267840 AT2G29150.1 28.235 255 167 7 14 264 16 258 3.35e-22 94.0
Msa1267840 AT3G46170.1 33.083 266 148 11 14 264 26 276 6.84e-22 93.6
Msa1267840 AT5G18210.1 30.483 269 153 9 14 265 8 259 3.10e-21 91.3
Msa1267840 AT5G06060.1 27.820 266 177 5 14 275 9 263 3.17e-21 91.3
Msa1267840 AT5G50700.1 33.503 197 119 4 12 206 43 229 4.52e-21 92.0
Msa1267840 AT5G50600.1 33.503 197 119 4 12 206 43 229 4.52e-21 92.0
Msa1267840 AT2G29370.1 27.344 256 171 5 14 265 16 260 4.93e-21 90.9
Msa1267840 AT3G51680.1 31.338 284 168 9 5 269 23 298 1.06e-20 90.5
Msa1267840 AT3G04000.1 29.787 282 173 8 1 267 1 272 1.34e-20 89.7
Msa1267840 AT1G07440.1 28.996 269 169 7 14 275 12 265 2.00e-20 89.0
Msa1267840 AT2G29360.1 27.652 264 178 7 14 274 16 269 2.01e-20 89.0
Msa1267840 AT4G03140.1 31.387 274 161 9 14 274 78 337 3.30e-20 89.7
Msa1267840 AT2G29350.1 26.953 256 169 6 14 264 15 257 4.10e-20 88.2
Msa1267840 AT2G29310.1 28.782 271 168 10 14 275 7 261 5.46e-20 87.8
Msa1267840 AT3G26770.1 28.030 264 166 9 14 265 41 292 1.41e-19 87.4
Msa1267840 AT2G29320.1 27.547 265 178 4 14 274 13 267 3.25e-19 85.9
Msa1267840 AT2G29340.1 29.032 279 181 7 14 288 7 272 6.89e-19 85.5
Msa1267840 AT4G13180.1 28.846 260 166 9 14 265 13 261 1.13e-18 84.0
Msa1267840 AT2G29330.1 31.250 272 158 10 14 274 7 260 1.53e-18 83.6
Msa1267840 AT2G29340.2 28.571 266 175 6 14 275 7 261 1.05e-17 81.3
Msa1267840 AT5G18210.2 30.827 266 148 10 14 260 8 256 1.58e-17 81.3
Msa1267840 AT1G07450.1 28.030 264 176 9 14 274 8 260 2.09e-17 80.5
Msa1267840 AT2G47140.1 29.545 264 167 9 14 270 6 257 2.27e-17 80.5
Msa1267840 AT1G24360.1 28.625 269 171 6 1 267 70 319 2.64e-17 81.3
Msa1267840 AT2G30670.1 27.881 269 173 7 14 275 7 261 7.98e-17 79.0
Msa1267840 AT2G47130.1 28.996 269 161 10 14 270 6 256 8.78e-17 79.0
Msa1267840 AT2G29290.1 28.099 242 163 7 35 274 11 243 1.77e-16 77.8
Msa1267840 AT2G47120.2 27.839 273 158 10 14 270 7 256 2.39e-16 77.4
Msa1267840 AT2G47120.1 27.839 273 158 10 14 270 6 255 2.67e-16 77.4
Msa1267840 AT2G29300.1 26.641 259 170 7 14 265 7 252 3.49e-16 77.0
Msa1267840 AT2G29300.2 26.641 259 170 7 14 265 7 252 4.17e-16 77.4
Msa1267840 AT3G29250.1 30.000 270 152 11 12 265 42 290 1.22e-15 76.3
Msa1267840 AT3G47350.1 31.188 202 119 6 12 207 42 229 1.98e-15 75.9
Msa1267840 AT3G29250.2 29.779 272 154 11 14 269 6 256 2.81e-15 74.7
Msa1267840 AT3G26760.1 27.174 276 173 9 14 274 36 298 2.89e-15 75.1
Msa1267840 AT3G47350.2 31.188 202 119 6 12 207 42 229 3.09e-15 75.5
Msa1267840 AT3G29250.3 30.224 268 150 11 14 265 42 288 4.67e-15 74.3
Msa1267840 AT5G50770.1 34.010 197 114 5 12 204 43 227 9.27e-15 73.9
Msa1267840 AT3G47360.1 29.534 193 124 5 17 207 48 230 2.11e-14 72.8
Msa1267840 AT3G03980.1 28.044 271 165 10 11 264 11 268 1.10e-13 70.1
Msa1267840 AT4G10020.1 28.351 194 129 4 7 198 40 225 2.77e-13 70.1
Msa1267840 AT3G29260.1 29.119 261 159 10 14 264 6 250 1.01e-12 67.4
Msa1267840 AT2G29310.2 26.275 255 163 10 30 275 21 259 1.67e-12 66.6
Msa1267840 AT3G42960.1 29.434 265 154 11 17 264 11 259 3.17e-12 65.9
Msa1267840 AT2G29320.2 25.100 251 170 6 30 274 11 249 6.09e-12 65.1
Msa1267840 AT3G47350.3 36.893 103 64 1 12 113 42 144 8.45e-12 64.7
Msa1267840 AT5G50590.1 28.141 199 131 4 12 208 43 231 3.90e-11 63.2
Msa1267840 AT5G50690.1 28.141 199 131 4 12 208 43 231 3.90e-11 63.2
Msa1267840 AT5G50590.2 28.141 199 131 4 12 208 43 231 6.57e-11 62.0
Msa1267840 AT5G50690.2 28.141 199 131 4 12 208 43 231 6.57e-11 62.0
Msa1267840 AT3G06060.2 29.612 206 126 10 14 214 54 245 7.17e-11 62.8
Msa1267840 AT3G06060.1 29.612 206 126 10 14 214 37 228 7.69e-11 62.4

Find 103 sgRNAs with CRISPR-Local

Find 311 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACACCAATTGGTTCATTTC+TGG 0.141423 8_3:-14772 None:intergenic
TGTGAAGGAAAATACATTAA+TGG 0.230658 8_3:+14651 Msa1267840:intron
CGCTTTCGATTCCTGCTGTT+GGG 0.266199 8_3:+10699 Msa1267840:CDS
ATTAGTGCTACCTTGCATTA+TGG 0.276429 8_3:+12336 Msa1267840:CDS
ATGCCAGGAGTGTCACTTAT+TGG 0.283238 8_3:-12698 None:intergenic
TTCAAACCAGAAATCTTAAA+AGG 0.319717 8_3:+10539 Msa1267840:CDS
ACTGGCGGAGCGTCGGGAAT+TGG 0.320337 8_3:+10578 Msa1267840:CDS
GGCAAAGTAGCTTTAATAAC+TGG 0.335212 8_3:+10560 Msa1267840:CDS
TGTATAAACTTGGGGAGAAA+TGG 0.343265 8_3:+12774 Msa1267840:CDS
CCTCTGCTACCTATTTCTTC+AGG 0.344151 8_3:-12734 None:intergenic
AAGAAACTTGGCACTGGAAT+GGG 0.348357 8_3:+12634 Msa1267840:CDS
CATCCAACAGAAGAATAAAA+AGG 0.351586 8_3:+14978 Msa1267840:three_prime_UTR
GTTTGAAATATCAACGCAAT+TGG 0.363203 8_3:+10601 Msa1267840:CDS
ATTCCTGCTGTTGGGTTTGA+GGG 0.366201 8_3:+10707 Msa1267840:CDS
CATAAATTATGTTTCTAAGT+TGG 0.368785 8_3:-14935 None:intergenic
AGTCACTTTCATTTCATGAT+TGG 0.373071 8_3:+15122 Msa1267840:three_prime_UTR
AGTTCTTCAATCTGCTGTTT+CGG 0.377117 8_3:+10667 Msa1267840:CDS
TTTGAAATATCAACGCAATT+GGG 0.380854 8_3:+10602 Msa1267840:CDS
TCGCTTTCGATTCCTGCTGT+TGG 0.383020 8_3:+10698 Msa1267840:CDS
CTGAGGATCTTTCGCCGAAT+GGG 0.391331 8_3:+10840 Msa1267840:CDS
TCTTTAAACAATACAATGAA+TGG 0.394168 8_3:+14858 Msa1267840:three_prime_UTR
TATTACATGTTGTGTTGTAT+TGG 0.394641 8_3:-10359 None:intergenic
GATTATGATATTCGAGTCAA+TGG 0.397358 8_3:+12662 Msa1267840:CDS
TTTGTACTGTCTTTGCCTGT+TGG 0.405774 8_3:+15025 Msa1267840:three_prime_UTR
TGGAGCGTCGGTTGCTGTAA+TGG 0.407877 8_3:+10631 Msa1267840:CDS
GATTCCTGCTGTTGGGTTTG+AGG 0.408705 8_3:+10706 Msa1267840:CDS
TTTAAGATTTCTGGTTTGAA+TGG 0.409904 8_3:-10536 None:intergenic
CACTCCTGGCATGAGTAAAC+TGG 0.411577 8_3:+12709 Msa1267840:CDS
AAACTGGCTCCTGAAGAAAT+AGG 0.417404 8_3:+12725 Msa1267840:CDS
GGCGACACTATGATAGTTGA+TGG 0.432277 8_3:+14672 Msa1267840:CDS
GTATAAACTTGGGGAGAAAT+GGG 0.441031 8_3:+12775 Msa1267840:CDS
TTTAATAACTGGCGGAGCGT+CGG 0.442410 8_3:+10571 Msa1267840:CDS
TTCTGGTTTGAATGGTGATT+CGG 0.443044 8_3:-10528 None:intergenic
GGCTATAGCTAGACCCCATA+TGG 0.445011 8_3:+14999 Msa1267840:three_prime_UTR
AGACAGTACAAACAACCATA+TGG 0.445723 8_3:-15014 None:intergenic
GACAGTACAAACAACCATAT+GGG 0.454081 8_3:-15013 None:intergenic
GCTGAGGATCTTTCGCCGAA+TGG 0.457228 8_3:+10839 Msa1267840:CDS
GGAGCGTCGGTTGCTGTAAT+GGG 0.457280 8_3:+10632 Msa1267840:CDS
ATTCTTGTTAATGCTGCTGC+TGG 0.464854 8_3:+10800 Msa1267840:CDS
ACCTGTCTCACTGCCTCCTT+CGG 0.468095 8_3:-14726 None:intergenic
CTCTTCTTAGCATCAGATGC+AGG 0.469651 8_3:+12815 Msa1267840:CDS
GAAATGCCTCTGTATAAACT+TGG 0.470825 8_3:+12764 Msa1267840:CDS
CACTTAAGTATCTCAAGAAA+GGG 0.475461 8_3:+12271 Msa1267840:CDS
AGATCCAGAAATGAACCAAT+TGG 0.476611 8_3:+14768 Msa1267840:CDS
CAAGAAACTTGGCACTGGAA+TGG 0.477594 8_3:+12633 Msa1267840:CDS
GCACTTAAGTATCTCAAGAA+AGG 0.480331 8_3:+12270 Msa1267840:CDS
AATTTGCTTACAGTTCCAAC+AGG 0.484607 8_3:-15040 None:intergenic
TCATGAAATGAAAGTGACTA+AGG 0.488125 8_3:-15118 None:intergenic
GCCGAAGGAGGCAGTGAGAC+AGG 0.494400 8_3:+14725 Msa1267840:CDS
AAATGCCTCTGTATAAACTT+GGG 0.505018 8_3:+12765 Msa1267840:CDS
TGATTCTACTACAAGAAACT+TGG 0.507418 8_3:+12622 Msa1267840:CDS
CCGCTAGAAGAGTTCCTACC+AGG 0.507529 8_3:-12297 None:intergenic
ATGGAGGACTCTGGCTGAGC+CGG 0.519343 8_3:+14691 Msa1267840:CDS
AGTAATCCCCTGCAGTGTAG+AGG 0.519773 8_3:-14835 None:intergenic
ATTATGATATTCGAGTCAAT+GGG 0.519870 8_3:+12663 Msa1267840:CDS
CTGGTAGGAACTCTTCTAGC+GGG 0.525188 8_3:+12298 Msa1267840:CDS
GGAGCCAGTTTACTCATGCC+AGG 0.525918 8_3:-12713 None:intergenic
CGAGTCAATGGGATTGCACC+AGG 0.526292 8_3:+12674 Msa1267840:CDS
TCACCAGTTCGAAACCCATT+CGG 0.528086 8_3:-10854 None:intergenic
CCTTGCATTATGGAGCTGCT+TGG 0.531021 8_3:+12346 Msa1267840:CDS
TACTACAAGAAACTTGGCAC+TGG 0.536669 8_3:+12628 Msa1267840:CDS
GCGAATTTAGCGTCGAAACA+TGG 0.537122 8_3:+10496 Msa1267840:exon
GTAATCCCCTGCAGTGTAGA+GGG 0.542642 8_3:-14834 None:intergenic
GGGGTTAAAACTGAATCTGC+TGG 0.551039 8_3:-14814 None:intergenic
AATTCATGTATCTGCAGCAA+AGG 0.552785 8_3:+12374 Msa1267840:CDS
TATCTCAAGAAAGGGGCACC+TGG 0.556489 8_3:+12279 Msa1267840:CDS
TTCTTGTTAATGCTGCTGCT+GGG 0.557653 8_3:+10801 Msa1267840:CDS
TCCTGCTGTTGGGTTTGAGG+GGG 0.558834 8_3:+10709 Msa1267840:CDS
GCCACGTTATTTGCCGAAGG+AGG 0.559286 8_3:+14713 Msa1267840:CDS
TTCCTGCTGTTGGGTTTGAG+GGG 0.564959 8_3:+10708 Msa1267840:CDS
TCAACGCAATTGGGGAAACA+TGG 0.567028 8_3:+10611 Msa1267840:CDS
TTGAAATATCAACGCAATTG+GGG 0.574459 8_3:+10603 Msa1267840:CDS
GGAGTGTCACTTATTGGACC+TGG 0.583283 8_3:-12692 None:intergenic
CCTGAAGAAATAGGTAGCAG+AGG 0.588385 8_3:+12734 Msa1267840:CDS
TGATAGTTGATGGAGGACTC+TGG 0.594047 8_3:+14682 Msa1267840:CDS
AGAAACTTGGCACTGGAATG+GGG 0.594972 8_3:+12635 Msa1267840:CDS
TCAAGAAAGGGGCACCTGGT+AGG 0.602664 8_3:+12283 Msa1267840:CDS
CCTTCGGCAAATAACGTGGC+CGG 0.605481 8_3:-14710 None:intergenic
TTTAACCCCTCTACACTGCA+GGG 0.606564 8_3:+14828 Msa1267840:three_prime_UTR
AATGCCTCTGTATAAACTTG+GGG 0.607132 8_3:+12766 Msa1267840:CDS
CGGTTGCTGTAATGGGTCGT+CGG 0.609341 8_3:+10639 Msa1267840:CDS
TGTGAGGAAGCAGGAAGATG+CGG 0.618720 8_3:+10733 Msa1267840:CDS
TTCTCCCCAAGTTTATACAG+AGG 0.622115 8_3:-12770 None:intergenic
GTAACTTTGCACGTGAAATG+TGG 0.627244 8_3:+15068 Msa1267840:three_prime_UTR
GTTTCATACACCAATGTGTG+TGG 0.630139 8_3:-10401 None:intergenic
TGGTAGGAACTCTTCTAGCG+GGG 0.631175 8_3:+12299 Msa1267840:CDS
CCAAGCAGCTCCATAATGCA+AGG 0.633376 8_3:-12346 None:intergenic
GGTCCAATAAGTGACACTCC+TGG 0.636505 8_3:+12695 Msa1267840:CDS
GCCTCCTTCGGCAAATAACG+TGG 0.636778 8_3:-14714 None:intergenic
GAATTGAATCAACGCAGTAG+CGG 0.640289 8_3:+10474 Msa1267840:five_prime_UTR
ACAGTACAAACAACCATATG+GGG 0.641921 8_3:-15012 None:intergenic
ATTGGGGAAACATGGAGCGT+CGG 0.642414 8_3:+10619 Msa1267840:CDS
GGAGAAATGGGATATTGCAA+TGG 0.644314 8_3:+12787 Msa1267840:CDS
TTAACCCCTCTACACTGCAG+GGG 0.652639 8_3:+14829 Msa1267840:three_prime_UTR
CCTGGTAGGAACTCTTCTAG+CGG 0.661737 8_3:+12297 Msa1267840:CDS
GGTAGGAACTCTTCTAGCGG+GGG 0.665696 8_3:+12300 Msa1267840:CDS
GTGTTGGATATCGATTCTGT+TGG 0.666144 8_3:+12225 Msa1267840:intron
AAAGTAGCTTTAATAACTGG+CGG 0.668949 8_3:+10563 Msa1267840:CDS
TGCACGTGAAATGTGGCTAG+TGG 0.675217 8_3:+15075 Msa1267840:three_prime_UTR
TAATCCCCTGCAGTGTAGAG+GGG 0.677656 8_3:-14833 None:intergenic
ACTTAAGTATCTCAAGAAAG+GGG 0.678718 8_3:+12272 Msa1267840:CDS
GTATAAGACGCCACACACAT+TGG 0.690444 8_3:+10391 Msa1267840:five_prime_UTR
GACACTATGATAGTTGATGG+AGG 0.701596 8_3:+14675 Msa1267840:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATGTAAATTTTAAAATCA+AGG - chr8_3:11730-11749 None:intergenic 10.0%
!!! ATTTTCATCTTTTTAATTTA+AGG + chr8_3:12422-12441 Msa1267840:intron 10.0%
!!! TTTTTAATTTAAGGATTTAA+AGG + chr8_3:12431-12450 Msa1267840:intron 10.0%
!!! ATTTTTTATTTATTTGTCTA+AGG + chr8_3:14606-14625 Msa1267840:intron 10.0%
!!! GCAAAATAATATATTTTCAT+TGG - chr8_3:12495-12514 None:intergenic 15.0%
!!! CTAAATTTTAATTATTCAAC+AGG + chr8_3:12592-12611 Msa1267840:intron 15.0%
!! ACAAGATATTATGTAATTAT+AGG + chr8_3:13097-13116 Msa1267840:intron 15.0%
!! CAAGATATTATGTAATTATA+GGG + chr8_3:13098-13117 Msa1267840:intron 15.0%
!!! TTTATGCATTATTTGTTTTA+TGG + chr8_3:13133-13152 Msa1267840:intron 15.0%
!! AATTTATATTTGTGGTATAA+GGG - chr8_3:13168-13187 None:intergenic 15.0%
!!! ACAAAACCTTTTTAAAATTT+GGG + chr8_3:14104-14123 Msa1267840:intron 15.0%
!!! ATTTGACTTTTAAGCATTTT+GGG + chr8_3:10768-10787 Msa1267840:CDS 20.0%
!! GAATCTAAAAAGTAAAATTG+AGG - chr8_3:10932-10951 None:intergenic 20.0%
!! ATTCAATTAACGTATGTATT+TGG + chr8_3:10948-10967 Msa1267840:intron 20.0%
!!! TGTCTATTTAGTAGTTATTT+TGG + chr8_3:11075-11094 Msa1267840:intron 20.0%
!! ATTTGAATTGCATAAAATCA+TGG + chr8_3:11757-11776 Msa1267840:intron 20.0%
!!! AAATTCTACAACTTTTTGAA+TGG - chr8_3:11872-11891 None:intergenic 20.0%
!!! AATGATTTTGCAATAATCTA+CGG - chr8_3:11931-11950 None:intergenic 20.0%
!! GTTAAAAGTAAGAGTTTAAT+AGG - chr8_3:12571-12590 None:intergenic 20.0%
!! CAATTTATATTTGTGGTATA+AGG - chr8_3:13169-13188 None:intergenic 20.0%
!! TATACCACAAATATAAATTG+TGG + chr8_3:13169-13188 Msa1267840:intron 20.0%
!! TATTGCAAAATTATGCAAAA+TGG - chr8_3:13215-13234 None:intergenic 20.0%
!! TTAAATAAAACATACGATCA+GGG - chr8_3:13450-13469 None:intergenic 20.0%
!! TTTAAATAAAACATACGATC+AGG - chr8_3:13451-13470 None:intergenic 20.0%
!!! GACAAAACCTTTTTAAAATT+TGG + chr8_3:14103-14122 Msa1267840:intron 20.0%
!!! ATGTAGTGTTTATAATGTTT+TGG - chr8_3:14177-14196 None:intergenic 20.0%
!! AAACATTATAAACACTACAT+AGG + chr8_3:14177-14196 Msa1267840:intron 20.0%
!! TTGATAAAATGAAATTTGCA+AGG + chr8_3:14405-14424 Msa1267840:intron 20.0%
!! AACAATCTGATAATATATGA+TGG - chr8_3:14472-14491 None:intergenic 20.0%
!! ACAATTGTTTAAATTTGTGA+AGG + chr8_3:14636-14655 Msa1267840:intron 20.0%
!! TCTTTAAACAATACAATGAA+TGG + chr8_3:14858-14877 Msa1267840:three_prime_UTR 20.0%
!!! CATAAATTATGTTTCTAAGT+TGG - chr8_3:14938-14957 None:intergenic 20.0%
!!! TTTAAGATTTCTGGTTTGAA+TGG - chr8_3:10539-10558 None:intergenic 25.0%
! TTCAAACCAGAAATCTTAAA+AGG + chr8_3:10539-10558 Msa1267840:CDS 25.0%
!! TTTGAAATATCAACGCAATT+GGG + chr8_3:10602-10621 Msa1267840:CDS 25.0%
!!! GATTTGACTTTTAAGCATTT+TGG + chr8_3:10767-10786 Msa1267840:CDS 25.0%
! CTAAAAAGTAAAATTGAGGA+CGG - chr8_3:10928-10947 None:intergenic 25.0%
! CATCAAATAGCTAATGTATA+TGG - chr8_3:10987-11006 None:intergenic 25.0%
! TAACAACATTCATACTACTA+AGG - chr8_3:11118-11137 None:intergenic 25.0%
! AATACATTTCTTAGAAATCG+AGG - chr8_3:11241-11260 None:intergenic 25.0%
! AGGCTAAACAACTTAAAAAA+AGG - chr8_3:11320-11339 None:intergenic 25.0%
!!! AGTTGTTTAGCCTTTTTAAA+CGG + chr8_3:11327-11346 Msa1267840:intron 25.0%
! ACTTACTTAACCGTTTAAAA+AGG - chr8_3:11340-11359 None:intergenic 25.0%
!!! AGCTTTGGTTAATAGATTTT+GGG + chr8_3:11384-11403 Msa1267840:intron 25.0%
!! TTATAAGTTTTTATCCAAGC+AGG + chr8_3:11634-11653 Msa1267840:intron 25.0%
!! TATAAGTTTTTATCCAAGCA+GGG + chr8_3:11635-11654 Msa1267840:intron 25.0%
! GTTAATCATGTTTAAATCTG+TGG - chr8_3:11708-11727 None:intergenic 25.0%
!! AACTTTTTGAATGGTTAACA+CGG - chr8_3:11863-11882 None:intergenic 25.0%
!! ATGCTATTTGAACATCAATT+TGG - chr8_3:11901-11920 None:intergenic 25.0%
! TGTAAATTAGATAAGAGTCT+TGG - chr8_3:12124-12143 None:intergenic 25.0%
!! CATTTCTCGTATTCAATTTT+AGG - chr8_3:12174-12193 None:intergenic 25.0%
! AAAGATGAAAATGAACATCT+AGG - chr8_3:12414-12433 None:intergenic 25.0%
!! AGTATAAATCAGTTTTACAC+TGG + chr8_3:12466-12485 Msa1267840:intron 25.0%
! ATTATGATATTCGAGTCAAT+GGG + chr8_3:12663-12682 Msa1267840:CDS 25.0%
!!! CTGTAAATGTTGTATTTTTG+TGG + chr8_3:12889-12908 Msa1267840:intron 25.0%
! AAGTTATGAAAATGTTTCCA+AGG - chr8_3:12929-12948 None:intergenic 25.0%
! CATGATTAATTAGTAGTGTT+CGG + chr8_3:12984-13003 Msa1267840:intron 25.0%
! TTATGTAATTATAGGGAAGT+AGG + chr8_3:13105-13124 Msa1267840:intron 25.0%
!!! TCATGATTTTTTTGACATGT+TGG + chr8_3:13295-13314 Msa1267840:intron 25.0%
! AGATGTATAAACTATGAGAA+GGG - chr8_3:13525-13544 None:intergenic 25.0%
!!! TTTCTAGGTGATTTTGTTTT+TGG - chr8_3:14055-14074 None:intergenic 25.0%
!!! AAAGTGAAAAGGTTATTTCT+AGG - chr8_3:14070-14089 None:intergenic 25.0%
!! GAAATAACCTTTTCACTTTA+CGG + chr8_3:14071-14090 Msa1267840:intron 25.0%
! TTAACTCAATCTTACAAAAC+CGG + chr8_3:14133-14152 Msa1267840:intron 25.0%
!! AAAACATAAGGTCAAGTTTT+GGG - chr8_3:14334-14353 None:intergenic 25.0%
!! AAAAACATAAGGTCAAGTTT+TGG - chr8_3:14335-14354 None:intergenic 25.0%
! ATAAAATGAAATTTGCAAGG+TGG + chr8_3:14408-14427 Msa1267840:intron 25.0%
! TGTGAAGGAAAATACATTAA+TGG + chr8_3:14651-14670 Msa1267840:intron 25.0%
!!! ATCCTTTTTAAGGTAAAATC+AGG - chr8_3:15185-15204 None:intergenic 25.0%
!!! ACTTTGCCTTTTAAGATTTC+TGG - chr8_3:10548-10567 None:intergenic 30.0%
!! AAAGTAGCTTTAATAACTGG+CGG + chr8_3:10563-10582 Msa1267840:CDS 30.0%
! GTTTGAAATATCAACGCAAT+TGG + chr8_3:10601-10620 Msa1267840:CDS 30.0%
TTGAAATATCAACGCAATTG+GGG + chr8_3:10603-10622 Msa1267840:CDS 30.0%
!!! AGGATTTTTGTTGTTTTCGA+TGG + chr8_3:10876-10895 Msa1267840:intron 30.0%
TTTGAATAAGTGTCTATACG+TGG + chr8_3:11165-11184 Msa1267840:intron 30.0%
TTGAATAAGTGTCTATACGT+GGG + chr8_3:11166-11185 Msa1267840:intron 30.0%
ATTTCTTAGAAATCGAGGTT+GGG - chr8_3:11236-11255 None:intergenic 30.0%
!! TTTGCTATTTCGATGCATTT+AGG + chr8_3:11259-11278 Msa1267840:intron 30.0%
!! TTGCTATTTCGATGCATTTA+GGG + chr8_3:11260-11279 Msa1267840:intron 30.0%
!! CAGCTTTGGTTAATAGATTT+TGG + chr8_3:11383-11402 Msa1267840:intron 30.0%
!! CTACTATCATAAGTTTTGTG+AGG + chr8_3:11436-11455 Msa1267840:intron 30.0%
!! ATAAGTTTTGTGAGGTTGTT+TGG + chr8_3:11444-11463 Msa1267840:intron 30.0%
!! TAAGTTTTGTGAGGTTGTTT+GGG + chr8_3:11445-11464 Msa1267840:intron 30.0%
!! GTTGTTTTCGTAAGCTATAT+AGG - chr8_3:11535-11554 None:intergenic 30.0%
AGCTTACGAAAACAACTTAT+AGG + chr8_3:11539-11558 Msa1267840:intron 30.0%
TTGCATAAAATCATGGTTGA+TGG + chr8_3:11764-11783 Msa1267840:intron 30.0%
ATCAGAATCCTTCTATAATG+TGG + chr8_3:12065-12084 Msa1267840:intron 30.0%
TTCATTATTGTTTGCAGTGT+TGG + chr8_3:12209-12228 Msa1267840:intron 30.0%
CACTTAAGTATCTCAAGAAA+GGG + chr8_3:12271-12290 Msa1267840:CDS 30.0%
ACTTAAGTATCTCAAGAAAG+GGG + chr8_3:12272-12291 Msa1267840:CDS 30.0%
TGATTCTACTACAAGAAACT+TGG + chr8_3:12622-12641 Msa1267840:CDS 30.0%
GATTATGATATTCGAGTCAA+TGG + chr8_3:12662-12681 Msa1267840:CDS 30.0%
AAATGCCTCTGTATAAACTT+GGG + chr8_3:12765-12784 Msa1267840:CDS 30.0%
ATTAATTAGTAGTGTTCGGT+TGG + chr8_3:12988-13007 Msa1267840:intron 30.0%
TTAATTAGTAGTGTTCGGTT+GGG + chr8_3:12989-13008 Msa1267840:intron 30.0%
! GTAGTGAAACTAGAAAGTAT+AGG + chr8_3:13071-13090 Msa1267840:intron 30.0%
TATATTTGTGGTATAAGGGT+AGG - chr8_3:13164-13183 None:intergenic 30.0%
GACACCACAATTTATATTTG+TGG - chr8_3:13176-13195 None:intergenic 30.0%
TGCAATACGTATTTAAACCT+TGG + chr8_3:13228-13247 Msa1267840:intron 30.0%
!! GTTATGTTTGTTTCTGTTCA+AGG + chr8_3:13413-13432 Msa1267840:intron 30.0%
GAGATGTATAAACTATGAGA+AGG - chr8_3:13526-13545 None:intergenic 30.0%
!! AATGAAGGGTATTGCAATAT+GGG - chr8_3:13628-13647 None:intergenic 30.0%
ACATGACTTAGTTGAATGAA+GGG - chr8_3:13642-13661 None:intergenic 30.0%
TTAACTGTCATCGAGTAATA+TGG - chr8_3:13795-13814 None:intergenic 30.0%
! TTGTCACAAATACTCTCTTT+TGG + chr8_3:13854-13873 Msa1267840:intron 30.0%
AAGTAACTACGTCCTAATAA+AGG - chr8_3:14002-14021 None:intergenic 30.0%
ACAAATACCGTAAAGTGAAA+AGG - chr8_3:14081-14100 None:intergenic 30.0%
!! GCTCTACCCAAATTTTAAAA+AGG - chr8_3:14113-14132 None:intergenic 30.0%
TGGAAATTCAACCTTCATTA+TGG - chr8_3:14296-14315 None:intergenic 30.0%
AGCATTCAGTGAGTAAAAAT+GGG - chr8_3:14512-14531 None:intergenic 30.0%
TAGCATTCAGTGAGTAAAAA+TGG - chr8_3:14513-14532 None:intergenic 30.0%
!!! ACCATGTAGTTGTTTTGAAA+TGG + chr8_3:14533-14552 Msa1267840:intron 30.0%
TCCATTTCAAAACAACTACA+TGG - chr8_3:14537-14556 None:intergenic 30.0%
!!! TTACTTTTTGGAACACCAAT+TGG - chr8_3:14786-14805 None:intergenic 30.0%
CATCCAACAGAAGAATAAAA+AGG + chr8_3:14978-14997 Msa1267840:three_prime_UTR 30.0%
! TAGCCTTTTTATTCTTCTGT+TGG - chr8_3:14984-15003 None:intergenic 30.0%
TCATGAAATGAAAGTGACTA+AGG - chr8_3:15121-15140 None:intergenic 30.0%
! AGTCACTTTCATTTCATGAT+TGG + chr8_3:15122-15141 Msa1267840:three_prime_UTR 30.0%
! AGCCTGATTTTACCTTAAAA+AGG + chr8_3:15180-15199 Msa1267840:three_prime_UTR 30.0%
! GGTTTATGCTATCCTTTTTA+AGG - chr8_3:15195-15214 None:intergenic 30.0%
!! TTCTGGTTTGAATGGTGATT+CGG - chr8_3:10531-10550 None:intergenic 35.0%
!! GGCAAAGTAGCTTTAATAAC+TGG + chr8_3:10560-10579 Msa1267840:CDS 35.0%
! AGTTCTTCAATCTGCTGTTT+CGG + chr8_3:10667-10686 Msa1267840:CDS 35.0%
! TGACTTTTAAGCATTTTGGG+AGG + chr8_3:10771-10790 Msa1267840:CDS 35.0%
AAAAGTAAAATTGAGGACGG+AGG - chr8_3:10925-10944 None:intergenic 35.0%
ACTAAGGAAAAACAGCTTTG+AGG - chr8_3:11102-11121 None:intergenic 35.0%
CATTTCTTAGAAATCGAGGT+TGG - chr8_3:11237-11256 None:intergenic 35.0%
!! TGCTATTTCGATGCATTTAG+GGG + chr8_3:11261-11280 Msa1267840:intron 35.0%
GGATACTAAAGACACTATGT+GGG - chr8_3:11299-11318 None:intergenic 35.0%
AGGATACTAAAGACACTATG+TGG - chr8_3:11300-11319 None:intergenic 35.0%
TGACTCACATGTAAATGAAG+TGG + chr8_3:11360-11379 Msa1267840:intron 35.0%
! TTGGTTAATAGATTTTGGGC+TGG + chr8_3:11388-11407 Msa1267840:intron 35.0%
!!! TAATAGATTTTGGGCTGGTT+TGG + chr8_3:11393-11412 Msa1267840:intron 35.0%
! ACTACTCATCTGAAACACAA+TGG - chr8_3:12097-12116 None:intergenic 35.0%
GCACTTAAGTATCTCAAGAA+AGG + chr8_3:12270-12289 Msa1267840:CDS 35.0%
ATTAGTGCTACCTTGCATTA+TGG + chr8_3:12336-12355 Msa1267840:CDS 35.0%
AATTCATGTATCTGCAGCAA+AGG + chr8_3:12374-12393 Msa1267840:CDS 35.0%
GAAATGCCTCTGTATAAACT+TGG + chr8_3:12764-12783 Msa1267840:CDS 35.0%
AATGCCTCTGTATAAACTTG+GGG + chr8_3:12766-12785 Msa1267840:CDS 35.0%
TGTATAAACTTGGGGAGAAA+TGG + chr8_3:12774-12793 Msa1267840:CDS 35.0%
GTATAAACTTGGGGAGAAAT+GGG + chr8_3:12775-12794 Msa1267840:CDS 35.0%
!! CTACTTCTCATTGACTTTGA+AGG - chr8_3:12952-12971 None:intergenic 35.0%
! TGGTTTTTGAATTCCATGCT+TGG + chr8_3:13018-13037 Msa1267840:intron 35.0%
! GGTTTTTGAATTCCATGCTT+GGG + chr8_3:13019-13038 Msa1267840:intron 35.0%
CATAGTTTATACATCTCACC+TGG + chr8_3:13529-13548 Msa1267840:intron 35.0%
AGCATTTGAAAGTCTATAGC+AGG - chr8_3:13563-13582 None:intergenic 35.0%
!! GAATGAAGGGTATTGCAATA+TGG - chr8_3:13629-13648 None:intergenic 35.0%
GACATGACTTAGTTGAATGA+AGG - chr8_3:13643-13662 None:intergenic 35.0%
! GAGAAAGGTTTTCACAACAA+AGG - chr8_3:13735-13754 None:intergenic 35.0%
! ACAAAGGCTCTTTAAGAGAA+AGG - chr8_3:13750-13769 None:intergenic 35.0%
TATTAAAGAGTGGAGGACAA+AGG - chr8_3:13766-13785 None:intergenic 35.0%
ATGGTACAGCTATTAAAGAG+TGG - chr8_3:13776-13795 None:intergenic 35.0%
ACACTGCATGAATTGTAACA+AGG - chr8_3:13895-13914 None:intergenic 35.0%
TATACAATTTCCTCCTGTGA+AGG + chr8_3:13919-13938 Msa1267840:intron 35.0%
!! CTGTAGGAGTATCCTTTATT+AGG + chr8_3:13987-14006 Msa1267840:intron 35.0%
! CTAAGGGTATACCATAATGA+AGG + chr8_3:14282-14301 Msa1267840:intron 35.0%
ATGATGGCAAAGATGAAACA+AGG - chr8_3:14456-14475 None:intergenic 35.0%
AGATCCAGAAATGAACCAAT+TGG + chr8_3:14768-14787 Msa1267840:CDS 35.0%
! AACACCAATTGGTTCATTTC+TGG - chr8_3:14775-14794 None:intergenic 35.0%
ACAGTACAAACAACCATATG+GGG - chr8_3:15015-15034 None:intergenic 35.0%
GACAGTACAAACAACCATAT+GGG - chr8_3:15016-15035 None:intergenic 35.0%
AGACAGTACAAACAACCATA+TGG - chr8_3:15017-15036 None:intergenic 35.0%
! AATTTGCTTACAGTTCCAAC+AGG - chr8_3:15043-15062 None:intergenic 35.0%
GTTTCATACACCAATGTGTG+TGG - chr8_3:10404-10423 None:intergenic 40.0%
GAATTGAATCAACGCAGTAG+CGG + chr8_3:10474-10493 Msa1267840:five_prime_UTR 40.0%
ATTCTTGTTAATGCTGCTGC+TGG + chr8_3:10800-10819 Msa1267840:CDS 40.0%
TTCTTGTTAATGCTGCTGCT+GGG + chr8_3:10801-10820 Msa1267840:CDS 40.0%
GTTGTTAAAGAGTCTCGCAT+CGG + chr8_3:11131-11150 Msa1267840:intron 40.0%
TGTAAATGAAGTGGCAGCTT+TGG + chr8_3:11369-11388 Msa1267840:intron 40.0%
TATCCAAGCAGGGTCATATT+AGG + chr8_3:11645-11664 Msa1267840:intron 40.0%
CAATGGCATAGTTCAATGAG+TGG - chr8_3:11824-11843 None:intergenic 40.0%
TTGTCTGTGACAAGTCTCAA+AGG - chr8_3:12020-12039 None:intergenic 40.0%
GGTCATTGCCACATTATAGA+AGG - chr8_3:12076-12095 None:intergenic 40.0%
!! GTGTTGGATATCGATTCTGT+TGG + chr8_3:12225-12244 Msa1267840:intron 40.0%
TACTACAAGAAACTTGGCAC+TGG + chr8_3:12628-12647 Msa1267840:CDS 40.0%
! AAGAAACTTGGCACTGGAAT+GGG + chr8_3:12634-12653 Msa1267840:CDS 40.0%
! AAACTGGCTCCTGAAGAAAT+AGG + chr8_3:12725-12744 Msa1267840:CDS 40.0%
TTCTCCCCAAGTTTATACAG+AGG - chr8_3:12773-12792 None:intergenic 40.0%
GGAGAAATGGGATATTGCAA+TGG + chr8_3:12787-12806 Msa1267840:CDS 40.0%
TTATCCAAGTGTTCCCTACT+TGG + chr8_3:12855-12874 Msa1267840:intron 40.0%
AGTATCGCATACACCAAGTA+GGG - chr8_3:12871-12890 None:intergenic 40.0%
TTTGTGGTATAAGGGTAGGA+AGG - chr8_3:13160-13179 None:intergenic 40.0%
GTAGAACTGAAAAGAAGCCA+AGG - chr8_3:13248-13267 None:intergenic 40.0%
!! TAACGCAGAGAAGCATTGAA+AGG - chr8_3:13270-13289 None:intergenic 40.0%
!! AAAGAAGTGCTGATGACATG+TGG - chr8_3:13669-13688 None:intergenic 40.0%
! AGAGAAAGGTTTTCACGAGA+TGG - chr8_3:13705-13724 None:intergenic 40.0%
! ACAAAGGCTCTCTAAGAGAA+AGG - chr8_3:13719-13738 None:intergenic 40.0%
GTACAGCTATTAAAGAGTGG+AGG - chr8_3:13773-13792 None:intergenic 40.0%
ATGAGACAGGAGAAGTAAAG+AGG - chr8_3:13830-13849 None:intergenic 40.0%
TTGTGACAAATGCATGAGAC+AGG - chr8_3:13843-13862 None:intergenic 40.0%
TCTCTCTTCAGCACTTCTAA+CGG + chr8_3:13961-13980 Msa1267840:intron 40.0%
TTCATTGTGTTGGGTGTGAA+GGG - chr8_3:14235-14254 None:intergenic 40.0%
! TTGGGAAGATATGGAAGTGT+TGG - chr8_3:14316-14335 None:intergenic 40.0%
!!! GGTCAAGTTTTGGGAAGATA+TGG - chr8_3:14325-14344 None:intergenic 40.0%
ATGGATGCATTTGTAACCCT+AGG + chr8_3:14552-14571 Msa1267840:intron 40.0%
! GTCACTGTTTGAGATACCTA+GGG - chr8_3:14571-14590 None:intergenic 40.0%
! AGTCACTGTTTGAGATACCT+AGG - chr8_3:14572-14591 None:intergenic 40.0%
!! GACACTATGATAGTTGATGG+AGG + chr8_3:14675-14694 Msa1267840:CDS 40.0%
!! CTGGCTCATAGCTTACTTTT+TGG - chr8_3:14798-14817 None:intergenic 40.0%
TTTGTACTGTCTTTGCCTGT+TGG + chr8_3:15025-15044 Msa1267840:three_prime_UTR 40.0%
GTAACTTTGCACGTGAAATG+TGG + chr8_3:15068-15087 Msa1267840:three_prime_UTR 40.0%
GTATAAGACGCCACACACAT+TGG + chr8_3:10391-10410 Msa1267840:five_prime_UTR 45.0%
! GCGAATTTAGCGTCGAAACA+TGG + chr8_3:10496-10515 Msa1267840:exon 45.0%
TTTAATAACTGGCGGAGCGT+CGG + chr8_3:10571-10590 Msa1267840:CDS 45.0%
TCAACGCAATTGGGGAAACA+TGG + chr8_3:10611-10630 Msa1267840:CDS 45.0%
ATTCCTGCTGTTGGGTTTGA+GGG + chr8_3:10707-10726 Msa1267840:CDS 45.0%
! GAATTTTCTTGCTGCTGCTG+AGG + chr8_3:10823-10842 Msa1267840:CDS 45.0%
TCACCAGTTCGAAACCCATT+CGG - chr8_3:10857-10876 None:intergenic 45.0%
!! ACCTCACATTCCGGTTTTGT+GGG + chr8_3:11199-11218 Msa1267840:intron 45.0%
CTAACACAACCCCACAAAAC+CGG - chr8_3:11212-11231 None:intergenic 45.0%
!!! GATTTTGGGCTGGTTTGGAT+TGG + chr8_3:11398-11417 Msa1267840:intron 45.0%
ACACCTAATATGACCCTGCT+TGG - chr8_3:11651-11670 None:intergenic 45.0%
ATGCCATTGCTAACCACATG+CGG + chr8_3:11835-11854 Msa1267840:intron 45.0%
CTAACGAGAGTAGAGTGATG+TGG - chr8_3:12517-12536 None:intergenic 45.0%
! CAAGAAACTTGGCACTGGAA+TGG + chr8_3:12633-12652 Msa1267840:CDS 45.0%
! AGAAACTTGGCACTGGAATG+GGG + chr8_3:12635-12654 Msa1267840:CDS 45.0%
!! ATGCCAGGAGTGTCACTTAT+TGG - chr8_3:12701-12720 None:intergenic 45.0%
! CCTCTGCTACCTATTTCTTC+AGG - chr8_3:12737-12756 None:intergenic 45.0%
CCTGAAGAAATAGGTAGCAG+AGG + chr8_3:12734-12753 Msa1267840:CDS 45.0%
CTCTTCTTAGCATCAGATGC+AGG + chr8_3:12815-12834 Msa1267840:CDS 45.0%
TACACCAAGTAGGGAACACT+TGG - chr8_3:12862-12881 None:intergenic 45.0%
CAGTATCGCATACACCAAGT+AGG - chr8_3:12872-12891 None:intergenic 45.0%
TGGTGACTATCTGAAAGCCT+TGG + chr8_3:12909-12928 Msa1267840:intron 45.0%
! TCCATGCTTGGGTTTTGCTT+TGG + chr8_3:13030-13049 Msa1267840:intron 45.0%
ACCAAAGCAAAACCCAAGCA+TGG - chr8_3:13034-13053 None:intergenic 45.0%
!! TTGGTGACAGTCTAGCTTTC+TGG + chr8_3:13049-13068 Msa1267840:intron 45.0%
CTATAGCAGGATTACAGACC+AGG - chr8_3:13550-13569 None:intergenic 45.0%
AGATGTTCATGCACCTTCAC+AGG - chr8_3:13935-13954 None:intergenic 45.0%
!! AGAAGTGCTGAAGAGAGACA+AGG - chr8_3:13959-13978 None:intergenic 45.0%
CTCAATCTTACAAAACCGGC+TGG + chr8_3:14137-14156 Msa1267840:intron 45.0%
TCTTACAAAACCGGCTGGTA+AGG + chr8_3:14142-14161 Msa1267840:intron 45.0%
ACTACATAGGCCGTATCTCT+AGG + chr8_3:14190-14209 Msa1267840:intron 45.0%
TCTCACACTGCCTAGAGATA+CGG - chr8_3:14203-14222 None:intergenic 45.0%
CTTCATTGTGTTGGGTGTGA+AGG - chr8_3:14236-14255 None:intergenic 45.0%
CTTCACACCCAACACAATGA+AGG + chr8_3:14234-14253 Msa1267840:intron 45.0%
TGGATCGCCTTCATTGTGTT+GGG - chr8_3:14244-14263 None:intergenic 45.0%
! ATCCACTTGCTGCAATTAGG+CGG + chr8_3:14259-14278 Msa1267840:intron 45.0%
GGCGACACTATGATAGTTGA+TGG + chr8_3:14672-14691 Msa1267840:CDS 45.0%
!! TGATAGTTGATGGAGGACTC+TGG + chr8_3:14682-14701 Msa1267840:CDS 45.0%
GGGGTTAAAACTGAATCTGC+TGG - chr8_3:14817-14836 None:intergenic 45.0%
! TTTTAACCCCTCTACACTGC+AGG + chr8_3:14827-14846 Msa1267840:three_prime_UTR 45.0%
TTTAACCCCTCTACACTGCA+GGG + chr8_3:14828-14847 Msa1267840:three_prime_UTR 45.0%
!! AAAAAAAAAAAAAAAACATA+AGG - chr8_3:14346-14365 None:intergenic 5.0%
!! ACAAATAAATAAAAAATTAA+AGG - chr8_3:14603-14622 None:intergenic 5.0%
! TTAATAACTGGCGGAGCGTC+GGG + chr8_3:10572-10591 Msa1267840:CDS 50.0%
ATTGGGGAAACATGGAGCGT+CGG + chr8_3:10619-10638 Msa1267840:CDS 50.0%
TCGCTTTCGATTCCTGCTGT+TGG + chr8_3:10698-10717 Msa1267840:CDS 50.0%
CGCTTTCGATTCCTGCTGTT+GGG + chr8_3:10699-10718 Msa1267840:CDS 50.0%
GATTCCTGCTGTTGGGTTTG+AGG + chr8_3:10706-10725 Msa1267840:CDS 50.0%
TTCCTGCTGTTGGGTTTGAG+GGG + chr8_3:10708-10727 Msa1267840:CDS 50.0%
TGTGAGGAAGCAGGAAGATG+CGG + chr8_3:10733-10752 Msa1267840:CDS 50.0%
CTGAGGATCTTTCGCCGAAT+GGG + chr8_3:10840-10859 Msa1267840:CDS 50.0%
! GAATGGGTTTCGAACTGGTG+AGG + chr8_3:10856-10875 Msa1267840:intron 50.0%
GCAATCCTCACCTCACATTC+CGG + chr8_3:11190-11209 Msa1267840:intron 50.0%
CAAAACCGGAATGTGAGGTG+AGG - chr8_3:11198-11217 None:intergenic 50.0%
! CACCTCACATTCCGGTTTTG+TGG + chr8_3:11198-11217 Msa1267840:intron 50.0%
!! CCTCACATTCCGGTTTTGTG+GGG + chr8_3:11200-11219 Msa1267840:intron 50.0%
TGCCATTGCTAACCACATGC+GGG + chr8_3:11836-11855 Msa1267840:intron 50.0%
!! TATCTCAAGAAAGGGGCACC+TGG + chr8_3:12279-12298 Msa1267840:CDS 50.0%
CCTGGTAGGAACTCTTCTAG+CGG + chr8_3:12297-12316 Msa1267840:CDS 50.0%
CTGGTAGGAACTCTTCTAGC+GGG + chr8_3:12298-12317 Msa1267840:CDS 50.0%
TGGTAGGAACTCTTCTAGCG+GGG + chr8_3:12299-12318 Msa1267840:CDS 50.0%
CCAAGCAGCTCCATAATGCA+AGG - chr8_3:12349-12368 None:intergenic 50.0%
CCTTGCATTATGGAGCTGCT+TGG + chr8_3:12346-12365 Msa1267840:CDS 50.0%
!! GGAGTGTCACTTATTGGACC+TGG - chr8_3:12695-12714 None:intergenic 50.0%
GGTCCAATAAGTGACACTCC+TGG + chr8_3:12695-12714 Msa1267840:CDS 50.0%
CACTCCTGGCATGAGTAAAC+TGG + chr8_3:12709-12728 Msa1267840:CDS 50.0%
TGTTCATGCACCTTCACAGG+AGG - chr8_3:13932-13951 None:intergenic 50.0%
! GCACTTCTAACGGATGCTGT+AGG + chr8_3:13971-13990 Msa1267840:intron 50.0%
AAAACCGGCTGGTAAGGTGA+GGG + chr8_3:14148-14167 Msa1267840:intron 50.0%
ATTGTGTTGGGTGTGAAGGG+TGG - chr8_3:14232-14251 None:intergenic 50.0%
GTGGATCGCCTTCATTGTGT+TGG - chr8_3:14245-14264 None:intergenic 50.0%
! GCGATCCACTTGCTGCAATT+AGG + chr8_3:14256-14275 Msa1267840:intron 50.0%
TTGCTGCAATTAGGCGGCTA+AGG + chr8_3:14265-14284 Msa1267840:intron 50.0%
TGCTGCAATTAGGCGGCTAA+GGG + chr8_3:14266-14285 Msa1267840:intron 50.0%
TTAACCCCTCTACACTGCAG+GGG + chr8_3:14829-14848 Msa1267840:three_prime_UTR 50.0%
TAATCCCCTGCAGTGTAGAG+GGG - chr8_3:14836-14855 None:intergenic 50.0%
GTAATCCCCTGCAGTGTAGA+GGG - chr8_3:14837-14856 None:intergenic 50.0%
AGTAATCCCCTGCAGTGTAG+AGG - chr8_3:14838-14857 None:intergenic 50.0%
GGCTATAGCTAGACCCCATA+TGG + chr8_3:14999-15018 Msa1267840:three_prime_UTR 50.0%
TGCACGTGAAATGTGGCTAG+TGG + chr8_3:15075-15094 Msa1267840:three_prime_UTR 50.0%
!! TGGAGCGTCGGTTGCTGTAA+TGG + chr8_3:10631-10650 Msa1267840:CDS 55.0%
!! GGAGCGTCGGTTGCTGTAAT+GGG + chr8_3:10632-10651 Msa1267840:CDS 55.0%
!! CGGTTGCTGTAATGGGTCGT+CGG + chr8_3:10639-10658 Msa1267840:CDS 55.0%
TCCTGCTGTTGGGTTTGAGG+GGG + chr8_3:10709-10728 Msa1267840:CDS 55.0%
TTGGGTTTGAGGGGGATGTG+AGG + chr8_3:10717-10736 Msa1267840:CDS 55.0%
GCTGAGGATCTTTCGCCGAA+TGG + chr8_3:10839-10858 Msa1267840:CDS 55.0%
! TCGCCGAATGGGTTTCGAAC+TGG + chr8_3:10851-10870 Msa1267840:CDS 55.0%
CCCCACAAAACCGGAATGTG+AGG - chr8_3:11203-11222 None:intergenic 55.0%
GTCCCGCATGTGGTTAGCAA+TGG - chr8_3:11841-11860 None:intergenic 55.0%
!! TCAAGAAAGGGGCACCTGGT+AGG + chr8_3:12283-12302 Msa1267840:CDS 55.0%
CCGCTAGAAGAGTTCCTACC+AGG - chr8_3:12300-12319 None:intergenic 55.0%
GGTAGGAACTCTTCTAGCGG+GGG + chr8_3:12300-12319 Msa1267840:CDS 55.0%
CGAGTCAATGGGATTGCACC+AGG + chr8_3:12674-12693 Msa1267840:CDS 55.0%
GGAGCCAGTTTACTCATGCC+AGG - chr8_3:12716-12735 None:intergenic 55.0%
AGTGTTCGGTTGGGACTTGC+TGG + chr8_3:12998-13017 Msa1267840:intron 55.0%
CAAAACCGGCTGGTAAGGTG+AGG + chr8_3:14147-14166 Msa1267840:intron 55.0%
GTAACCCTCACCTTACCAGC+CGG - chr8_3:14155-14174 None:intergenic 55.0%
AGCCGCCTAATTGCAGCAAG+TGG - chr8_3:14264-14283 None:intergenic 55.0%
CCTTCGGCAAATAACGTGGC+CGG - chr8_3:14713-14732 None:intergenic 55.0%
GCCACGTTATTTGCCGAAGG+AGG + chr8_3:14713-14732 Msa1267840:CDS 55.0%
GCCTCCTTCGGCAAATAACG+TGG - chr8_3:14717-14736 None:intergenic 55.0%
ACCTGTCTCACTGCCTCCTT+CGG - chr8_3:14729-14748 None:intergenic 55.0%
TCCCCCTCAAACCCAACAGC+AGG - chr8_3:10713-10732 None:intergenic 60.0%
TGAGGGGGATGTGAGGAAGC+AGG + chr8_3:10724-10743 Msa1267840:CDS 60.0%
GGTTAACACGGTCCCGCATG+TGG - chr8_3:11851-11870 None:intergenic 60.0%
ATGGAGGACTCTGGCTGAGC+CGG + chr8_3:14691-14710 Msa1267840:CDS 60.0%
CCGGCCACGTTATTTGCCGA+AGG + chr8_3:14710-14729 Msa1267840:CDS 60.0%
!! ACTGGCGGAGCGTCGGGAAT+TGG + chr8_3:10578-10597 Msa1267840:CDS 65.0%
!! CTGGCGGAGCGTCGGGAATT+GGG + chr8_3:10579-10598 Msa1267840:CDS 65.0%
GCCGAAGGAGGCAGTGAGAC+AGG + chr8_3:14725-14744 Msa1267840:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 10360 15214 10360 ID=Msa1267840;Name=Msa1267840
chr8_3 mRNA 10360 15214 10360 ID=Msa1267840-mRNA-1;Parent=Msa1267840;Name=Msa1267840-mRNA-1;_AED=0.00;_eAED=0.00;_QI=155|1|1|1|1|1|4|402|301
chr8_3 exon 10360 10872 10360 ID=Msa1267840-mRNA-1:exon:6;Parent=Msa1267840-mRNA-1
chr8_3 exon 12226 12395 12226 ID=Msa1267840-mRNA-1:exon:7;Parent=Msa1267840-mRNA-1
chr8_3 exon 12614 12836 12614 ID=Msa1267840-mRNA-1:exon:8;Parent=Msa1267840-mRNA-1
chr8_3 exon 14658 15214 14658 ID=Msa1267840-mRNA-1:exon:9;Parent=Msa1267840-mRNA-1
chr8_3 five_prime_UTR 10360 10514 10360 ID=Msa1267840-mRNA-1:five_prime_utr;Parent=Msa1267840-mRNA-1
chr8_3 CDS 10515 10872 10515 ID=Msa1267840-mRNA-1:cds;Parent=Msa1267840-mRNA-1
chr8_3 CDS 12226 12395 12226 ID=Msa1267840-mRNA-1:cds;Parent=Msa1267840-mRNA-1
chr8_3 CDS 12614 12836 12614 ID=Msa1267840-mRNA-1:cds;Parent=Msa1267840-mRNA-1
chr8_3 CDS 14658 14812 14658 ID=Msa1267840-mRNA-1:cds;Parent=Msa1267840-mRNA-1
chr8_3 three_prime_UTR 14813 15214 14813 ID=Msa1267840-mRNA-1:three_prime_utr;Parent=Msa1267840-mRNA-1
Gene Sequence

>Msa1267840

ATGGTAGCTTCAACCGAATCACCATTCAAACCAGAAATCTTAAAAGGCAAAGTAGCTTTAATAACTGGCGGAGCGTCGGGAATTGGGTTTGAAATATCAACGCAATTGGGGAAACATGGAGCGTCGGTTGCTGTAATGGGTCGTCGGAAGCAAGTTCTTCAATCTGCTGTTTCGGTTCTTCAATCGCTTTCGATTCCTGCTGTTGGGTTTGAGGGGGATGTGAGGAAGCAGGAAGATGCGGCGAGAGTTGTTGATTTGACTTTTAAGCATTTTGGGAGGATTGATATTCTTGTTAATGCTGCTGCTGGGAATTTTCTTGCTGCTGCTGAGGATCTTTCGCCGAATGGGTTTCGAACTGTGTTGGATATCGATTCTGTTGGCACATTCACTATGTGTCATGAAGCACTTAAGTATCTCAAGAAAGGGGCACCTGGTAGGAACTCTTCTAGCGGGGGATTAATAATAAACATTAGTGCTACCTTGCATTATGGAGCTGCTTGGTATCAAATTCATGTATCTGCAGCAAAGGCAGCTGTTGATTCTACTACAAGAAACTTGGCACTGGAATGGGGAACAGATTATGATATTCGAGTCAATGGGATTGCACCAGGTCCAATAAGTGACACTCCTGGCATGAGTAAACTGGCTCCTGAAGAAATAGGTAGCAGAGGCAGAGATGAAATGCCTCTGTATAAACTTGGGGAGAAATGGGATATTGCAATGGCTGCTCTCTTCTTAGCATCAGATGCAGGAAAATACATTAATGGCGACACTATGATAGTTGATGGAGGACTCTGGCTGAGCCGGCCACGTTATTTGCCGAAGGAGGCAGTGAGACAGGTATCGCGAGAAGTAGAAAAAAGATCCAGAAATGAACCAATTGGTGTTCCAAAAAGTAAGCTATGA
Protein sequence

>Msa1267840

MVASTESPFKPEILKGKVALITGGASGIGFEISTQLGKHGASVAVMGRRKQVLQSAVSVLQSLSIPAVGFEGDVRKQEDAARVVDLTFKHFGRIDILVNAAAGNFLAAAEDLSPNGFRTVLDIDSVGTFTMCHEALKYLKKGAPGRNSSSGGLIINISATLHYGAAWYQIHVSAAKAAVDSTTRNLALEWGTDYDIRVNGIAPGPISDTPGMSKLAPEEIGSRGRDEMPLYKLGEKWDIAMAALFLASDAGKYINGDTMIVDGGLWLSRPRYLPKEAVRQVSREVEKRSRNEPIGVPKSKL*