AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0621650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0621650 MtrunA17_Chr2g0286891 98.068 880 16 1 1 879 1 880 0.0 1760
Msa0621650 MtrunA17_Chr1g0196471 34.550 767 382 19 117 876 75 728 8.74e-111 356
Msa0621650 MtrunA17_Chr4g0054731 42.297 357 193 4 518 874 389 732 2.00e-70 247
Msa0621650 MtrunA17_Chr4g0054731 43.624 149 62 2 118 245 77 224 5.29e-30 127
Msa0621650 MtrunA17_Chr4g0064071 40.667 150 52 1 518 667 126 238 9.80e-21 92.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0621650 AT4G03000.1 37.361 902 435 20 5 878 14 813 3.19e-156 478
Msa0621650 AT4G03000.2 37.361 902 435 20 5 878 14 813 3.19e-156 478
Msa0621650 AT1G03365.1 37.233 889 444 21 15 879 24 822 1.61e-144 448
Msa0621650 AT1G03365.2 37.233 889 444 21 15 879 24 822 1.61e-144 448
Msa0621650 AT4G03000.4 34.765 722 345 19 5 698 14 637 4.48e-94 310
Msa0621650 AT4G03000.6 34.765 722 345 19 5 698 14 637 4.48e-94 310
Msa0621650 AT4G03000.3 34.765 722 345 19 5 698 14 637 1.58e-93 310
Msa0621650 AT4G03000.5 34.765 722 345 19 5 698 14 637 3.20e-93 310
Msa0621650 AT2G35330.2 41.341 358 192 5 521 874 363 706 5.59e-73 254
Msa0621650 AT2G35330.2 43.038 158 76 4 118 264 71 225 2.81e-28 122
Msa0621650 AT2G35330.1 41.341 358 192 5 521 874 390 733 7.19e-73 254
Msa0621650 AT2G35330.1 43.038 158 76 4 118 264 98 252 4.29e-28 121
Msa0621650 AT1G32530.1 36.798 356 207 5 523 874 365 706 1.51e-50 190
Msa0621650 AT1G32530.1 39.259 135 75 1 118 245 69 203 1.34e-23 107

Find 199 sgRNAs with CRISPR-Local

Find 308 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAGCACTCTTTAAGTTTA+TGG 0.104247 4_2:-73869012 None:intergenic
TATATTCCCAATTGCTTATA+AGG 0.172965 4_2:+73866617 None:intergenic
GTCGAAATTACCCCATGTTT+TGG 0.242112 4_2:+73867979 Msa0621650:CDS
TTGATTTGTTTGCAGCTAAA+TGG 0.242428 4_2:+73867786 Msa0621650:intron
TCGCTGCATAAACTACTTAA+AGG 0.246936 4_2:-73868546 None:intergenic
GATCTGAATGCATTATTCTA+TGG 0.247944 4_2:-73871028 None:intergenic
CCCATGATTGAAACTTCATT+TGG 0.283994 4_2:-73869769 None:intergenic
AGAAGTTGAACGACTTAAAA+AGG 0.284434 4_2:+73869532 Msa0621650:CDS
TAGCTTCAAGGACACGAAAA+AGG 0.285731 4_2:+73870681 Msa0621650:CDS
TCGGTTTCTGTTCAAGAAAA+AGG 0.294425 4_2:+73867847 Msa0621650:CDS
TGCAAGATTGTCCTTCTTGC+AGG 0.326643 4_2:+73870905 Msa0621650:CDS
TTCAGGGCTGATCCACCTTT+AGG 0.327941 4_2:+73867889 Msa0621650:CDS
TTAATAGCCTTATAAGCAAT+TGG 0.329372 4_2:-73866624 None:intergenic
TGAAGATGGTTCCGAGGGTT+CGG 0.332368 4_2:+73869393 Msa0621650:CDS
CAATTTGATCCTCTCCTCCT+TGG 0.339110 4_2:-73870524 None:intergenic
AGAATAATGCATTCAGATCT+TGG 0.340113 4_2:+73871032 Msa0621650:three_prime_UTR
GTCCATTATTAGATGAGAAA+TGG 0.349080 4_2:-73869088 None:intergenic
TTGTGCTATGTGTTGAGCTT+GGG 0.352708 4_2:-73866653 None:intergenic
ACCCATTTCTCATCTAATAA+TGG 0.356305 4_2:+73869086 Msa0621650:CDS
CCATGATTGAAACTTCATTT+GGG 0.356844 4_2:-73869768 None:intergenic
TCAGGGCTGATCCACCTTTA+GGG 0.358652 4_2:+73867890 Msa0621650:CDS
GTAGAAATAAGAGAAAATTC+AGG 0.361206 4_2:+73867872 Msa0621650:CDS
GAATTACATCTTGGCCGAAT+TGG 0.379171 4_2:+73868416 Msa0621650:CDS
GAATCAGAAATCGTTGTTAC+AGG 0.382061 4_2:+73869793 Msa0621650:CDS
TCAAATTCGCGGTGGACTCT+TGG 0.382780 4_2:+73868713 Msa0621650:CDS
GAAGATGGTTCCGAGGGTTC+GGG 0.388461 4_2:+73869394 Msa0621650:CDS
CATGGGAGATTGAGAGGTTC+CGG 0.393131 4_2:-73868790 None:intergenic
CTTGCATTCCCTGCTTCTCA+TGG 0.393218 4_2:-73870888 None:intergenic
TCTCTTTCATTGTCTTCAAA+AGG 0.400450 4_2:+73869251 Msa0621650:CDS
CAAGGAGGAGAGGATCAAAT+TGG 0.404486 4_2:+73870525 Msa0621650:CDS
GACAAGTCATCTAATGCTTC+CGG 0.404497 4_2:+73868771 Msa0621650:CDS
TCTGTAGCTGATAATGCATC+TGG 0.405226 4_2:-73869218 None:intergenic
GATTTCTCAGATTGAGTGCC+TGG 0.408635 4_2:-73868672 None:intergenic
TAGAATCCAGGCTAAATGTT+GGG 0.410933 4_2:-73869118 None:intergenic
TTAAGACCTGGCATCTGTTA+TGG 0.412665 4_2:+73868282 Msa0621650:CDS
AGGCTAAATGTTGGGGTAGA+TGG 0.414356 4_2:-73869110 None:intergenic
TTCTGAGATGGAAAATGCCT+TGG 0.415309 4_2:+73869592 Msa0621650:CDS
ATTGTATTACTGAGATTTAG+AGG 0.418899 4_2:+73867109 Msa0621650:five_prime_UTR
GGACTACTGACTGCTGGTAT+AGG 0.422320 4_2:-73869194 None:intergenic
CTGCAGTTTCATCTGAGGTC+AGG 0.425266 4_2:+73868082 Msa0621650:CDS
TTTGTGCTATGTGTTGAGCT+TGG 0.430969 4_2:-73866654 None:intergenic
CAGCAGAGAATTCATAACTT+AGG 0.431980 4_2:-73867951 None:intergenic
TTCTTGCAGGAGCCCCATCC+AGG 0.433423 4_2:+73870918 Msa0621650:CDS
TAACAGTCAAATGTCTTCTT+CGG 0.438296 4_2:+73867828 Msa0621650:CDS
TAATAGCCTTATAAGCAATT+GGG 0.440927 4_2:-73866623 None:intergenic
AGATGAGTTGATTCTGAAGA+TGG 0.442092 4_2:+73869379 Msa0621650:CDS
CCCAAATGAAGTTTCAATCA+TGG 0.443366 4_2:+73869768 Msa0621650:CDS
AAGCTATGCCGGTAGCTTCA+AGG 0.448095 4_2:+73870669 Msa0621650:CDS
ATGCATTCAGATCTTGGATC+TGG 0.448154 4_2:+73871038 Msa0621650:three_prime_UTR
CGCTGCATAAACTACTTAAA+GGG 0.448414 4_2:-73868545 None:intergenic
TATAAACATAAGCAAGGCAG+TGG 0.448971 4_2:+73869034 Msa0621650:CDS
ACAGAAATTCGCTCCTGGAT+GGG 0.450372 4_2:-73870931 None:intergenic
TGTCTCATGGACTTGTCATA+TGG 0.455279 4_2:-73869335 None:intergenic
TTGAAGACAATGAAAGAGAA+AGG 0.455334 4_2:-73869247 None:intergenic
CAGAAATTCGCTCCTGGATG+GGG 0.459004 4_2:-73870930 None:intergenic
CGAGCTTCAGAATTACATCT+TGG 0.459109 4_2:+73868407 Msa0621650:CDS
CACCGCGTCTATTTCAGAAC+TGG 0.460689 4_2:+73870726 Msa0621650:CDS
GGCTGCTCGTCGACTGAGTA+AGG 0.463847 4_2:+73869472 Msa0621650:CDS
TTGAAGAACGCAAACCAATT+CGG 0.464489 4_2:-73868430 None:intergenic
TTGAGTCCCATGGCGCTGAT+TGG 0.468361 4_2:+73868136 Msa0621650:CDS
ACTTGTTATTACAGCAGATA+GGG 0.468592 4_2:-73869289 None:intergenic
TATCGGACATACGGGTCTAA+AGG 0.468738 4_2:+73868909 Msa0621650:CDS
TCCATTATTAGATGAGAAAT+GGG 0.470325 4_2:-73869087 None:intergenic
ATCGGACATACGGGTCTAAA+GGG 0.472374 4_2:+73868910 Msa0621650:CDS
CAATGAGCTCCATGAGAAGC+AGG 0.473165 4_2:+73870879 Msa0621650:CDS
ATCTACCCCAACATTTAGCC+TGG 0.474786 4_2:+73869112 Msa0621650:CDS
GATGGCTTGAAGCTCGACCT+TGG 0.477990 4_2:+73868048 Msa0621650:CDS
TTTCGTGTCCTTGAAGCTAC+CGG 0.478278 4_2:-73870677 None:intergenic
TGGTTTGAGTGGTTTAATCC+TGG 0.479762 4_2:+73868956 Msa0621650:CDS
CCCTCTTGGTGCTACTCGAA+TGG 0.480575 4_2:-73868845 None:intergenic
GGAATTGAATTACAAGGACT+TGG 0.484319 4_2:-73868648 None:intergenic
GATCAAATTGGAAGCGGAGA+AGG 0.484375 4_2:+73870537 Msa0621650:CDS
GACTTGTTATTACAGCAGAT+AGG 0.486606 4_2:-73869290 None:intergenic
TTTGCGTTCTTCAAGAGGTT+AGG 0.489276 4_2:+73868439 Msa0621650:CDS
GTTAGCCACGGTTGTTCTGA+TGG 0.489726 4_2:+73868030 Msa0621650:CDS
TTCTGAAATAGACGCGGTGT+GGG 0.490115 4_2:-73870722 None:intergenic
GGCAAATCAAAAGGTGATGC+AGG 0.491344 4_2:+73869451 Msa0621650:CDS
GACAGAGTGGGCAAATCAAA+AGG 0.495082 4_2:+73869442 Msa0621650:CDS
TCTGAGATGGAAAATGCCTT+GGG 0.495498 4_2:+73869593 Msa0621650:CDS
CACAGAAATTCGCTCCTGGA+TGG 0.502512 4_2:-73870932 None:intergenic
CTCTTACTTGACTCCCCTAA+AGG 0.502873 4_2:-73867904 None:intergenic
AAAATGCGGGATTGTTAGAA+AGG 0.503581 4_2:+73868821 Msa0621650:CDS
TAGAAATAAGAGAAAATTCA+GGG 0.503963 4_2:+73867873 Msa0621650:CDS
TGCAAAATGAACTTCAAGAA+TGG 0.504403 4_2:+73869420 Msa0621650:CDS
ATCACAGGCTAAACGGCGGC+AGG 0.504970 4_2:+73870420 Msa0621650:intron
CAGGGCTGATCCACCTTTAG+GGG 0.505454 4_2:+73867891 Msa0621650:CDS
GAACGGAGGAGGTGTGAAAC+GGG 0.507987 4_2:+73870774 Msa0621650:CDS
ATATATTACTTTCAATGCAA+AGG 0.510068 4_2:+73871075 Msa0621650:three_prime_UTR
ATCTGAATGCATTATTCTAT+GGG 0.513965 4_2:-73871027 None:intergenic
CTGACCTCAGATGAAACTGC+AGG 0.516905 4_2:-73868081 None:intergenic
ATCACTCCAATCAGCGCCAT+GGG 0.517228 4_2:-73868142 None:intergenic
CCAAATGAAGTTTCAATCAT+GGG 0.518136 4_2:+73869769 Msa0621650:CDS
CTTCTAATTCCTCCTTAGGC+TGG 0.519710 4_2:-73868110 None:intergenic
GAGGAGGTGTGAAACGGGAA+AGG 0.521023 4_2:+73870779 Msa0621650:CDS
GGAATCGATGGAAGCTATGC+CGG 0.523339 4_2:+73870658 Msa0621650:CDS
GAAAATGCCTTGGGTAAAGC+AGG 0.524830 4_2:+73869602 Msa0621650:CDS
AACGCACAGAAATTCGCTCC+TGG 0.525525 4_2:-73870936 None:intergenic
AGGAGGTGTGAAACGGGAAA+GGG 0.525773 4_2:+73870780 Msa0621650:CDS
TTGCAGCTAAATGGCATCAC+TGG 0.527853 4_2:+73867795 Msa0621650:intron
CTGTAGCTGATAATGCATCT+GGG 0.529420 4_2:-73869217 None:intergenic
TTGTAGCATGGGAGATTGAG+AGG 0.530282 4_2:-73868796 None:intergenic
AGTTTATGCAGCGATGGCAT+TGG 0.532146 4_2:+73868555 Msa0621650:CDS
TGAACGGAGGAGGTGTGAAA+CGG 0.533156 4_2:+73870773 Msa0621650:CDS
TGCAGCGATGGCATTGGTGA+TGG 0.533644 4_2:+73868561 Msa0621650:CDS
ATTTCTCAGATTGAGTGCCT+GGG 0.536057 4_2:-73868671 None:intergenic
CTAGAATCCAGGCTAAATGT+TGG 0.536588 4_2:-73869119 None:intergenic
TTAAATAACTTCTCTATGAA+CGG 0.538843 4_2:+73870757 Msa0621650:CDS
CACTGAAGATGTTGCTACAA+AGG 0.538959 4_2:+73868254 Msa0621650:CDS
GGAAAGCTGAGTGGTTTGAG+TGG 0.539286 4_2:+73868945 Msa0621650:CDS
TTGGGGCAACCATTGTCTCA+TGG 0.541430 4_2:-73869348 None:intergenic
AAGAGAACAAATTGAGGAGT+CGG 0.541615 4_2:+73870495 Msa0621650:CDS
TGTGCTATGTGTTGAGCTTG+GGG 0.543136 4_2:-73866652 None:intergenic
GCTGTGACTTGCCCAAAACA+TGG 0.543592 4_2:-73867991 None:intergenic
AAATAGTATCACTAGAATCC+AGG 0.544420 4_2:-73869130 None:intergenic
GGTCAGGCTTAGCCAGCCTA+AGG 0.548390 4_2:+73868098 Msa0621650:CDS
AAGTCACATAGTAGTTGTGT+GGG 0.551659 4_2:+73869308 Msa0621650:CDS
AACAGAAGAACTTCTCTCCA+TGG 0.554042 4_2:+73870453 Msa0621650:CDS
TGAGGAGTCGGCTAGAACCA+AGG 0.554113 4_2:+73870507 Msa0621650:CDS
AAAAGAAAGAGAACAAATTG+AGG 0.556625 4_2:+73870489 Msa0621650:CDS
AACTTCTCTATGAACGGAGG+AGG 0.562509 4_2:+73870763 Msa0621650:CDS
ATTGGTTTGCGTTCTTCAAG+AGG 0.562855 4_2:+73868434 Msa0621650:CDS
AGGCTGAGCTTAAGACACTA+AGG 0.563919 4_2:+73869498 Msa0621650:CDS
CTGTGACTTGCCCAAAACAT+GGG 0.565277 4_2:-73867990 None:intergenic
AGTGCAAGCGGAGCAATTCG+AGG 0.565932 4_2:+73869877 Msa0621650:CDS
TTGCATCCATAACAGATGCC+AGG 0.570859 4_2:-73868288 None:intergenic
ACCACTTCTAATTCCTCCTT+AGG 0.570887 4_2:-73868114 None:intergenic
GGAGAGGATCAAATTGGAAG+CGG 0.573756 4_2:+73870531 Msa0621650:CDS
CGGCTAGAACCAAGGAGGAG+AGG 0.575165 4_2:+73870515 Msa0621650:CDS
CAAGTCACATAGTAGTTGTG+TGG 0.580362 4_2:+73869307 Msa0621650:CDS
TTAAAATAGTGCTCTCGTGA+AGG 0.587307 4_2:-73868375 None:intergenic
GATCACTCCAATCAGCGCCA+TGG 0.587469 4_2:-73868143 None:intergenic
TGCAGTACGGAAGCTCGAGA+TGG 0.587948 4_2:+73869649 Msa0621650:CDS
TTAGAAGTGGTTGAGTCCCA+TGG 0.588976 4_2:+73868126 Msa0621650:CDS
CTTGTTATTACAGCAGATAG+GGG 0.590136 4_2:-73869288 None:intergenic
TTGTCCACCTGCTTTACCCA+AGG 0.591973 4_2:-73869609 None:intergenic
TGGATGGGGCTCCTGCAAGA+AGG 0.592363 4_2:-73870916 None:intergenic
TGCTGCATTGAGGAAAGAGA+TGG 0.592537 4_2:+73869676 Msa0621650:CDS
TTAAGTAGTTTATGCAGCGA+TGG 0.592929 4_2:+73868549 Msa0621650:CDS
CAGGCTTAGCCAGCCTAAGG+AGG 0.593792 4_2:+73868101 Msa0621650:CDS
ATCAGCAACAAACTTTCAAG+AGG 0.595207 4_2:+73869724 Msa0621650:CDS
AAAGCAAGCCGAAGTGCAAG+CGG 0.595548 4_2:+73869865 Msa0621650:CDS
GACTTGTGCACTGTTAGCCA+CGG 0.596382 4_2:+73868018 Msa0621650:CDS
GGGTAAAGCAGGTGGACAAG+TGG 0.599397 4_2:+73869613 Msa0621650:CDS
GTTCTGAAATAGACGCGGTG+TGG 0.599691 4_2:-73870723 None:intergenic
GGAGTGTGTGATGTGCCTTT+CGG 0.601614 4_2:+73870801 Msa0621650:CDS
CAAGATGTAATTCTGAAGCT+CGG 0.607013 4_2:-73868406 None:intergenic
GCAGAAAATTCTCAAATTCG+CGG 0.611943 4_2:+73868702 Msa0621650:CDS
TGATTCTGAAGATGGTTCCG+AGG 0.613147 4_2:+73869387 Msa0621650:CDS
CGTGTCACATGCTTGTGCAA+TGG 0.613972 4_2:+73868515 Msa0621650:CDS
TTTCTCAGATTGAGTGCCTG+GGG 0.616223 4_2:-73868670 None:intergenic
GCTATGTGTTGAGCTTGGGG+AGG 0.616848 4_2:-73866649 None:intergenic
AGAATCCAGGCTAAATGTTG+GGG 0.617379 4_2:-73869117 None:intergenic
CTGCAGGGCTAGATAATCCA+AGG 0.619620 4_2:-73868065 None:intergenic
GATTCTGAAGATGGTTCCGA+GGG 0.620356 4_2:+73869388 Msa0621650:CDS
GCCTAAGGAGGAATTAGAAG+TGG 0.621473 4_2:+73868113 Msa0621650:CDS
TAGCCCTGCAGTTTCATCTG+AGG 0.622105 4_2:+73868077 Msa0621650:CDS
TCACATGCTTGTGCAATGGA+TGG 0.625176 4_2:+73868519 Msa0621650:CDS
TGTAGCTGATAATGCATCTG+GGG 0.627271 4_2:-73869216 None:intergenic
AGATGGAGAATGCTGCATTG+AGG 0.628423 4_2:+73869666 Msa0621650:CDS
TGCTTCTATAAACATAAGCA+AGG 0.629751 4_2:+73869028 Msa0621650:CDS
TTACTTGACTCCCCTAAAGG+TGG 0.630430 4_2:-73867901 None:intergenic
CAAATTGGCACAGATATCGA+AGG 0.631352 4_2:+73869838 Msa0621650:CDS
ATGACAAGTCCATGAGACAA+TGG 0.637572 4_2:+73869339 Msa0621650:CDS
CAAACAACTTGATGTGCACA+TGG 0.640114 4_2:-73870847 None:intergenic
AACTTCAAGAATGGACAGAG+TGG 0.640249 4_2:+73869429 Msa0621650:CDS
ATGGCATCACTGGTTGTCAG+TGG 0.643711 4_2:+73867805 Msa0621650:CDS
CCATTCGAGTAGCACCAAGA+GGG 0.645720 4_2:+73868845 Msa0621650:CDS
GGGTCTAAAGGGTCTTCAAG+AGG 0.646096 4_2:+73868921 Msa0621650:CDS
GAATTGCTCCGCTTGCACTT+CGG 0.649365 4_2:-73869873 None:intergenic
AATGCCTTGGGTAAAGCAGG+TGG 0.652750 4_2:+73869605 Msa0621650:CDS
TCCATTCGAGTAGCACCAAG+AGG 0.654219 4_2:+73868844 Msa0621650:CDS
ACTTCAAGAATGGACAGAGT+GGG 0.655947 4_2:+73869430 Msa0621650:CDS
ATAAACATAAGCAAGGCAGT+GGG 0.657479 4_2:+73869035 Msa0621650:CDS
TGACCTCAGATGAAACTGCA+GGG 0.658780 4_2:-73868080 None:intergenic
TGTGACTTGCCCAAAACATG+GGG 0.662318 4_2:-73867989 None:intergenic
TAAGATCGATCTTAGAACAT+CGG 0.662677 4_2:-73866759 None:intergenic
TCTAAAGGGTCTTCAAGAGG+AGG 0.662790 4_2:+73868924 Msa0621650:CDS
CAACAGACATCTCTTCCGAA+AGG 0.663930 4_2:-73870816 None:intergenic
AACGAGCTAATACTGCAGTA+CGG 0.665440 4_2:+73869636 Msa0621650:CDS
ACAAAGGCTATGTTAAGACC+TGG 0.665472 4_2:+73868270 Msa0621650:CDS
TTCTCAGATTGAGTGCCTGG+GGG 0.666069 4_2:-73868669 None:intergenic
TCAGTTTCGGAGATGCAATG+TGG 0.669957 4_2:+73868469 Msa0621650:CDS
TCTGAAATAGACGCGGTGTG+GGG 0.671611 4_2:-73870721 None:intergenic
GAAAATTCTCAAATTCGCGG+TGG 0.674479 4_2:+73868705 Msa0621650:CDS
TCAATCTGAGAAATCCGTTG+TGG 0.679156 4_2:+73868680 Msa0621650:CDS
AATGAGCTCCATGAGAAGCA+GGG 0.679279 4_2:+73870880 Msa0621650:CDS
TCGTCGACTGAGTAAGGACA+AGG 0.681425 4_2:+73869478 Msa0621650:CDS
TGCATTGAGGAAAGAGATGG+AGG 0.682188 4_2:+73869679 Msa0621650:CDS
GGAGGCTGCTAAGTTACGTG+CGG 0.686850 4_2:+73869697 Msa0621650:CDS
AACCAGTTCTGAAATAGACG+CGG 0.688246 4_2:-73870728 None:intergenic
TCAAGAGGAGGAAAGCTGAG+TGG 0.691060 4_2:+73868936 Msa0621650:CDS
GTAGCTGATAATGCATCTGG+GGG 0.692281 4_2:-73869215 None:intergenic
AATAACTTCTCTATGAACGG+AGG 0.693247 4_2:+73870760 Msa0621650:CDS
TCAAGCCATCAGAACAACCG+TGG 0.719058 4_2:-73868035 None:intergenic
AGTCACATAGTAGTTGTGTG+GGG 0.726877 4_2:+73869309 Msa0621650:CDS
TCGTCAGAAGTCAATCCACG+TGG 0.728301 4_2:+73868878 Msa0621650:CDS
AAATATCACAGGCTAAACGG+CGG 0.736276 4_2:+73870416 Msa0621650:intron
ATTATTCTATGGGAGTCCCG+AGG 0.739410 4_2:-73871017 None:intergenic
GGAGTCGGCTAGAACCAAGG+AGG 0.754094 4_2:+73870510 Msa0621650:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAAAAATAAAATAAACAG+AGG + chr4_2:73866673-73866692 Msa0621650:five_prime_UTR 15.0%
!!! GAAAAATCAATGTTTTTTTT+AGG - chr4_2:73866728-73866747 None:intergenic 15.0%
!! TTATGTAATTGAATTCAATT+GGG + chr4_2:73866952-73866971 Msa0621650:intron 15.0%
!!! AGTTTTTAATTTTTTTCAAC+TGG + chr4_2:73866982-73867001 Msa0621650:intron 15.0%
!!! GTTTTTAATTTTTTTCAACT+GGG + chr4_2:73866983-73867002 Msa0621650:intron 15.0%
!!! AAATTTTCATATAACTACTT+AGG - chr4_2:73870141-73870160 None:intergenic 15.0%
!! GTTATGTAATTGAATTCAAT+TGG + chr4_2:73866951-73866970 Msa0621650:intron 20.0%
!!! TTTTTTAATCTGATTGTTTC+AGG + chr4_2:73867070-73867089 Msa0621650:intron 20.0%
!!! ATAATGCTATCAAATTTGTT+GGG + chr4_2:73867239-73867258 Msa0621650:intron 20.0%
!! TAGAAATAAGAGAAAATTCA+GGG + chr4_2:73867873-73867892 Msa0621650:CDS 20.0%
!!! ATAAAAAAAATTGTTGCTTG+TGG + chr4_2:73868228-73868247 Msa0621650:CDS 20.0%
!! AAAAGCTGAAATAAAAAGTT+CGG + chr4_2:73868614-73868633 Msa0621650:CDS 20.0%
!! AATGACTGAAAAAAACAAAT+TGG + chr4_2:73869823-73869842 Msa0621650:CDS 20.0%
!! TAAATGTTGGTATTAATATC+TGG - chr4_2:73869954-73869973 None:intergenic 20.0%
!! ATATAACTACTTAGGTATAA+AGG - chr4_2:73870133-73870152 None:intergenic 20.0%
!! TTAAATAACTTCTCTATGAA+CGG + chr4_2:73870757-73870776 Msa0621650:CDS 20.0%
!! ATATATTACTTTCAATGCAA+AGG + chr4_2:73871075-73871094 Msa0621650:three_prime_UTR 20.0%
!!! TAATAGCCTTATAAGCAATT+GGG - chr4_2:73866626-73866645 None:intergenic 25.0%
!!! TTAATAGCCTTATAAGCAAT+TGG - chr4_2:73866627-73866646 None:intergenic 25.0%
!! GTTTGTCAATTTTCTTCAAT+CGG + chr4_2:73866791-73866810 Msa0621650:five_prime_UTR 25.0%
!! TTTGTCAATTTTCTTCAATC+GGG + chr4_2:73866792-73866811 Msa0621650:five_prime_UTR 25.0%
!! ACTCTGAATTTTTCTTCAAT+TGG + chr4_2:73866848-73866867 Msa0621650:intron 25.0%
!! CTCTGAATTTTTCTTCAATT+GGG + chr4_2:73866849-73866868 Msa0621650:intron 25.0%
!! TCTGAATTTTTCTTCAATTG+GGG + chr4_2:73866850-73866869 Msa0621650:intron 25.0%
! ATTGTATTACTGAGATTTAG+AGG + chr4_2:73867109-73867128 Msa0621650:five_prime_UTR 25.0%
! GCATTATCAATCAACAAAAA+GGG - chr4_2:73867226-73867245 None:intergenic 25.0%
! AGCATTATCAATCAACAAAA+AGG - chr4_2:73867227-73867246 None:intergenic 25.0%
! GATAATGCTATCAAATTTGT+TGG + chr4_2:73867238-73867257 Msa0621650:intron 25.0%
! TCTTTCAAGCTATGTAAATA+TGG + chr4_2:73867698-73867717 Msa0621650:intron 25.0%
!!! AATCACAAGTTGATTAGTTT+CGG - chr4_2:73867725-73867744 None:intergenic 25.0%
! GTAGAAATAAGAGAAAATTC+AGG + chr4_2:73867872-73867891 Msa0621650:CDS 25.0%
!! TTTTATTGCACTCTTGAAAA+TGG - chr4_2:73868214-73868233 None:intergenic 25.0%
!!! CTGATTTAAGTTTTTTATCC+AGG - chr4_2:73868977-73868996 None:intergenic 25.0%
! TCCATTATTAGATGAGAAAT+GGG - chr4_2:73869090-73869109 None:intergenic 25.0%
! GTAATGTTAAATGAATCACT+TGG + chr4_2:73870293-73870312 Msa0621650:intron 25.0%
! TTGTAAAGGAAAAATATCAC+AGG + chr4_2:73870405-73870424 Msa0621650:intron 25.0%
! AAAAGAAAGAGAACAAATTG+AGG + chr4_2:73870489-73870508 Msa0621650:CDS 25.0%
!!! CAATTTCTTTTTCAAGTTTC+TGG - chr4_2:73870588-73870607 None:intergenic 25.0%
! ATCTGAATGCATTATTCTAT+GGG - chr4_2:73871030-73871049 None:intergenic 25.0%
TAAGATCGATCTTAGAACAT+CGG - chr4_2:73866762-73866781 None:intergenic 30.0%
! AGATTACTTGTGCATGTTTT+TGG + chr4_2:73867018-73867037 Msa0621650:intron 30.0%
! GATTACTTGTGCATGTTTTT+GGG + chr4_2:73867019-73867038 Msa0621650:intron 30.0%
TGGCAAAATGAAAATCAACA+AGG - chr4_2:73867271-73867290 None:intergenic 30.0%
CAAGTACTATCAAAACGTAA+TGG - chr4_2:73867291-73867310 None:intergenic 30.0%
!! CATTACGTTTTGATAGTACT+TGG + chr4_2:73867289-73867308 Msa0621650:intron 30.0%
!! GTTTTGATAGTACTTGGTTT+TGG + chr4_2:73867295-73867314 Msa0621650:intron 30.0%
!! ATCACAAGTTGATTAGTTTC+GGG - chr4_2:73867724-73867743 None:intergenic 30.0%
!! TTGATTTGTTTGCAGCTAAA+TGG + chr4_2:73867786-73867805 Msa0621650:intron 30.0%
TAACAGTCAAATGTCTTCTT+CGG + chr4_2:73867828-73867847 Msa0621650:CDS 30.0%
!!! ACTTTAGCTCTTTTGAGAAA+CGG + chr4_2:73868339-73868358 Msa0621650:CDS 30.0%
GAAAAAAGTTATCGGACATA+CGG + chr4_2:73868900-73868919 Msa0621650:CDS 30.0%
AAAAAAGTTATCGGACATAC+GGG + chr4_2:73868901-73868920 Msa0621650:CDS 30.0%
!! AGAAGCACTCTTTAAGTTTA+TGG - chr4_2:73869015-73869034 None:intergenic 30.0%
TGCTTCTATAAACATAAGCA+AGG + chr4_2:73869028-73869047 Msa0621650:CDS 30.0%
ACCCATTTCTCATCTAATAA+TGG + chr4_2:73869086-73869105 Msa0621650:CDS 30.0%
GTCCATTATTAGATGAGAAA+TGG - chr4_2:73869091-73869110 None:intergenic 30.0%
AAATAGTATCACTAGAATCC+AGG - chr4_2:73869133-73869152 None:intergenic 30.0%
TTGAAGACAATGAAAGAGAA+AGG - chr4_2:73869250-73869269 None:intergenic 30.0%
TCTCTTTCATTGTCTTCAAA+AGG + chr4_2:73869251-73869270 Msa0621650:CDS 30.0%
!! ACTTGTTATTACAGCAGATA+GGG - chr4_2:73869292-73869311 None:intergenic 30.0%
! AACTCATCTTTTCTATCTTG+GGG - chr4_2:73869368-73869387 None:intergenic 30.0%
! CAACTCATCTTTTCTATCTT+GGG - chr4_2:73869369-73869388 None:intergenic 30.0%
! TCAACTCATCTTTTCTATCT+TGG - chr4_2:73869370-73869389 None:intergenic 30.0%
TGCAAAATGAACTTCAAGAA+TGG + chr4_2:73869420-73869439 Msa0621650:CDS 30.0%
AGAAGTTGAACGACTTAAAA+AGG + chr4_2:73869532-73869551 Msa0621650:CDS 30.0%
! TTTTCATGGTGTTTTCTTCA+AGG - chr4_2:73869568-73869587 None:intergenic 30.0%
CCATGATTGAAACTTCATTT+GGG - chr4_2:73869771-73869790 None:intergenic 30.0%
CCAAATGAAGTTTCAATCAT+GGG + chr4_2:73869769-73869788 Msa0621650:CDS 30.0%
TGAAAACAAATAACAGCATC+AGG - chr4_2:73869979-73869998 None:intergenic 30.0%
!! TGCTGTTATTTGTTTTCATG+TGG + chr4_2:73869981-73870000 Msa0621650:intron 30.0%
TACATGTTGGTATTAATACC+TGG - chr4_2:73870064-73870083 None:intergenic 30.0%
AACATCATTTCCTTGTTGAA+TGG + chr4_2:73870266-73870285 Msa0621650:intron 30.0%
ACATCATTTCCTTGTTGAAT+GGG + chr4_2:73870267-73870286 Msa0621650:intron 30.0%
! CTTAATGAGTTTGTGTTACT+GGG + chr4_2:73870372-73870391 Msa0621650:intron 30.0%
GAAAAATATCACAGGCTAAA+CGG + chr4_2:73870413-73870432 Msa0621650:intron 30.0%
AAAATTGCTGCACTAAAAAG+AGG + chr4_2:73870637-73870656 Msa0621650:CDS 30.0%
!! AGTGTTTTGAATAATGTCCT+CGG + chr4_2:73871000-73871019 Msa0621650:three_prime_UTR 30.0%
GATCTGAATGCATTATTCTA+TGG - chr4_2:73871031-73871050 None:intergenic 30.0%
AGAATAATGCATTCAGATCT+TGG + chr4_2:73871032-73871051 Msa0621650:three_prime_UTR 30.0%
GTATAGCTGTCTAATGATGA+TGG - chr4_2:73867609-73867628 None:intergenic 35.0%
TCGGTTTCTGTTCAAGAAAA+AGG + chr4_2:73867847-73867866 Msa0621650:CDS 35.0%
CAGCAGAGAATTCATAACTT+AGG - chr4_2:73867954-73867973 None:intergenic 35.0%
! TCGAAATTACCCCATGTTTT+GGG + chr4_2:73867980-73867999 Msa0621650:CDS 35.0%
TTAAAATAGTGCTCTCGTGA+AGG - chr4_2:73868378-73868397 None:intergenic 35.0%
!! CAAGATGTAATTCTGAAGCT+CGG - chr4_2:73868409-73868428 None:intergenic 35.0%
TTGAAGAACGCAAACCAATT+CGG - chr4_2:73868433-73868452 None:intergenic 35.0%
!!! GTTAGGCCATTTTTCAGTTT+CGG + chr4_2:73868456-73868475 Msa0621650:CDS 35.0%
CGCTGCATAAACTACTTAAA+GGG - chr4_2:73868548-73868567 None:intergenic 35.0%
TCGCTGCATAAACTACTTAA+AGG - chr4_2:73868549-73868568 None:intergenic 35.0%
TTAAGTAGTTTATGCAGCGA+TGG + chr4_2:73868549-73868568 Msa0621650:CDS 35.0%
!! CTTTTGATTGTGATTCAGTC+TGG - chr4_2:73868599-73868618 None:intergenic 35.0%
GGAATTGAATTACAAGGACT+TGG - chr4_2:73868651-73868670 None:intergenic 35.0%
! TTGAGAATTTTCTGCCACAA+CGG - chr4_2:73868697-73868716 None:intergenic 35.0%
GCAGAAAATTCTCAAATTCG+CGG + chr4_2:73868702-73868721 Msa0621650:CDS 35.0%
! GAAAAACAGAGTGCTAATTC+TGG + chr4_2:73868735-73868754 Msa0621650:CDS 35.0%
AAAATGCGGGATTGTTAGAA+AGG + chr4_2:73868821-73868840 Msa0621650:CDS 35.0%
TCCACGTGGAAAAAAGTTAT+CGG + chr4_2:73868892-73868911 Msa0621650:CDS 35.0%
TATAAACATAAGCAAGGCAG+TGG + chr4_2:73869034-73869053 Msa0621650:CDS 35.0%
ATAAACATAAGCAAGGCAGT+GGG + chr4_2:73869035-73869054 Msa0621650:CDS 35.0%
TAGAATCCAGGCTAAATGTT+GGG - chr4_2:73869121-73869140 None:intergenic 35.0%
!! CTTGTTATTACAGCAGATAG+GGG - chr4_2:73869291-73869310 None:intergenic 35.0%
!! GACTTGTTATTACAGCAGAT+AGG - chr4_2:73869293-73869312 None:intergenic 35.0%
AAGTCACATAGTAGTTGTGT+GGG + chr4_2:73869308-73869327 Msa0621650:CDS 35.0%
!! AGATGAGTTGATTCTGAAGA+TGG + chr4_2:73869379-73869398 Msa0621650:CDS 35.0%
!!! CATCTCAGAAAGCTTTTTCA+TGG - chr4_2:73869582-73869601 None:intergenic 35.0%
! CATGAAAAAGCTTTCTGAGA+TGG + chr4_2:73869580-73869599 Msa0621650:CDS 35.0%
ATCAGCAACAAACTTTCAAG+AGG + chr4_2:73869724-73869743 Msa0621650:CDS 35.0%
CCCAAATGAAGTTTCAATCA+TGG + chr4_2:73869768-73869787 Msa0621650:CDS 35.0%
CCCATGATTGAAACTTCATT+TGG - chr4_2:73869772-73869791 None:intergenic 35.0%
GAATCAGAAATCGTTGTTAC+AGG + chr4_2:73869793-73869812 Msa0621650:CDS 35.0%
ACAGCATCAGGAATAAATGT+TGG - chr4_2:73869967-73869986 None:intergenic 35.0%
CAAAGCAGTTGGAATAAAAC+AGG - chr4_2:73870023-73870042 None:intergenic 35.0%
ACAGCTTGAGTAATACATGT+TGG - chr4_2:73870077-73870096 None:intergenic 35.0%
TAAGAGCAGAAAATGAGTGT+GGG - chr4_2:73870173-73870192 None:intergenic 35.0%
TTAAGAGCAGAAAATGAGTG+TGG - chr4_2:73870174-73870193 None:intergenic 35.0%
ATTGAATGTGTTTGCATCTG+TGG - chr4_2:73870343-73870362 None:intergenic 35.0%
GCTTAATGAGTTTGTGTTAC+TGG + chr4_2:73870371-73870390 Msa0621650:intron 35.0%
! TTTTCGTATAGAACTGACCA+TGG - chr4_2:73870473-73870492 None:intergenic 35.0%
AAGAGAACAAATTGAGGAGT+CGG + chr4_2:73870495-73870514 Msa0621650:CDS 35.0%
!! TGCAGCAATTTTTGAAGAGT+CGG - chr4_2:73870629-73870648 None:intergenic 35.0%
AATAACTTCTCTATGAACGG+AGG + chr4_2:73870760-73870779 Msa0621650:CDS 35.0%
!! GTGTTTTGAATAATGTCCTC+GGG + chr4_2:73871001-73871020 Msa0621650:three_prime_UTR 35.0%
TTGTGCTATGTGTTGAGCTT+GGG - chr4_2:73866656-73866675 None:intergenic 40.0%
TTTGTGCTATGTGTTGAGCT+TGG - chr4_2:73866657-73866676 None:intergenic 40.0%
TTCTTCAATTGGGGATCTTC+TGG + chr4_2:73866859-73866878 Msa0621650:intron 40.0%
TCTTCAATTGGGGATCTTCT+GGG + chr4_2:73866860-73866879 Msa0621650:intron 40.0%
!!! GTACTTGGTTTTGGAATCAG+TGG + chr4_2:73867304-73867323 Msa0621650:intron 40.0%
GAATGCTTGTGAATTGATGC+TGG + chr4_2:73867332-73867351 Msa0621650:intron 40.0%
! GTCGAAATTACCCCATGTTT+TGG + chr4_2:73867979-73867998 Msa0621650:CDS 40.0%
ACCACTTCTAATTCCTCCTT+AGG - chr4_2:73868117-73868136 None:intergenic 40.0%
CACTGAAGATGTTGCTACAA+AGG + chr4_2:73868254-73868273 Msa0621650:CDS 40.0%
ACAAAGGCTATGTTAAGACC+TGG + chr4_2:73868270-73868289 Msa0621650:CDS 40.0%
TTAAGACCTGGCATCTGTTA+TGG + chr4_2:73868282-73868301 Msa0621650:CDS 40.0%
CGAGCTTCAGAATTACATCT+TGG + chr4_2:73868407-73868426 Msa0621650:CDS 40.0%
GAATTACATCTTGGCCGAAT+TGG + chr4_2:73868416-73868435 Msa0621650:CDS 40.0%
!! ATTGGTTTGCGTTCTTCAAG+AGG + chr4_2:73868434-73868453 Msa0621650:CDS 40.0%
TTTGCGTTCTTCAAGAGGTT+AGG + chr4_2:73868439-73868458 Msa0621650:CDS 40.0%
GCATCTCCGAAACTGAAAAA+TGG - chr4_2:73868465-73868484 None:intergenic 40.0%
ATTTCTCAGATTGAGTGCCT+GGG - chr4_2:73868674-73868693 None:intergenic 40.0%
TCAATCTGAGAAATCCGTTG+TGG + chr4_2:73868680-73868699 Msa0621650:CDS 40.0%
GAAAATTCTCAAATTCGCGG+TGG + chr4_2:73868705-73868724 Msa0621650:CDS 40.0%
GACAAGTCATCTAATGCTTC+CGG + chr4_2:73868771-73868790 Msa0621650:CDS 40.0%
! CCGCATTTTTCTTGTAGCAT+GGG - chr4_2:73868810-73868829 None:intergenic 40.0%
CCCATGCTACAAGAAAAATG+CGG + chr4_2:73868807-73868826 Msa0621650:CDS 40.0%
CCATGCTACAAGAAAAATGC+GGG + chr4_2:73868808-73868827 Msa0621650:CDS 40.0%
ACGAAAAATGTACTCCCTCT+TGG - chr4_2:73868862-73868881 None:intergenic 40.0%
!!! TCCGATAACTTTTTTCCACG+TGG - chr4_2:73868896-73868915 None:intergenic 40.0%
TGGTTTGAGTGGTTTAATCC+TGG + chr4_2:73868956-73868975 Msa0621650:CDS 40.0%
AGAATCCAGGCTAAATGTTG+GGG - chr4_2:73869120-73869139 None:intergenic 40.0%
CTAGAATCCAGGCTAAATGT+TGG - chr4_2:73869122-73869141 None:intergenic 40.0%
! TGTAGCTGATAATGCATCTG+GGG - chr4_2:73869219-73869238 None:intergenic 40.0%
! CTGTAGCTGATAATGCATCT+GGG - chr4_2:73869220-73869239 None:intergenic 40.0%
! TCTGTAGCTGATAATGCATC+TGG - chr4_2:73869221-73869240 None:intergenic 40.0%
CAAGTCACATAGTAGTTGTG+TGG + chr4_2:73869307-73869326 Msa0621650:CDS 40.0%
AGTCACATAGTAGTTGTGTG+GGG + chr4_2:73869309-73869328 Msa0621650:CDS 40.0%
TGTCTCATGGACTTGTCATA+TGG - chr4_2:73869338-73869357 None:intergenic 40.0%
ATGACAAGTCCATGAGACAA+TGG + chr4_2:73869339-73869358 Msa0621650:CDS 40.0%
AACTTCAAGAATGGACAGAG+TGG + chr4_2:73869429-73869448 Msa0621650:CDS 40.0%
ACTTCAAGAATGGACAGAGT+GGG + chr4_2:73869430-73869449 Msa0621650:CDS 40.0%
!! TTCTGAGATGGAAAATGCCT+TGG + chr4_2:73869592-73869611 Msa0621650:CDS 40.0%
! TCTGAGATGGAAAATGCCTT+GGG + chr4_2:73869593-73869612 Msa0621650:CDS 40.0%
AACGAGCTAATACTGCAGTA+CGG + chr4_2:73869636-73869655 Msa0621650:CDS 40.0%
CAAATTGGCACAGATATCGA+AGG + chr4_2:73869838-73869857 Msa0621650:CDS 40.0%
ATTCCAAATGCTTTGCCATG+CGG + chr4_2:73869918-73869937 Msa0621650:intron 40.0%
AAAATGCATGGCAAAGCAGT+TGG - chr4_2:73870034-73870053 None:intergenic 40.0%
CCTGGTGAAGAAAAAATGCA+TGG - chr4_2:73870046-73870065 None:intergenic 40.0%
!!! CCATGCATTTTTTCTTCACC+AGG + chr4_2:73870043-73870062 Msa0621650:intron 40.0%
! CAAGCTGTTGTTTGTTCTCA+CGG + chr4_2:73870089-73870108 Msa0621650:intron 40.0%
! AAGCTGTTGTTTGTTCTCAC+GGG + chr4_2:73870090-73870109 Msa0621650:intron 40.0%
TCATTTCCTTGTTGAATGGG+TGG + chr4_2:73870270-73870289 Msa0621650:intron 40.0%
CATTTCCTTGTTGAATGGGT+GGG + chr4_2:73870271-73870290 Msa0621650:intron 40.0%
TGGGTCTGTTCTCATTGTAA+AGG + chr4_2:73870391-73870410 Msa0621650:intron 40.0%
AAATATCACAGGCTAAACGG+CGG + chr4_2:73870416-73870435 Msa0621650:intron 40.0%
AACAGAAGAACTTCTCTCCA+TGG + chr4_2:73870453-73870472 Msa0621650:CDS 40.0%
GCACTAAAAAGAGGAATCGA+TGG + chr4_2:73870646-73870665 Msa0621650:CDS 40.0%
TAGCTTCAAGGACACGAAAA+AGG + chr4_2:73870681-73870700 Msa0621650:CDS 40.0%
AGCTTCAAGGACACGAAAAA+GGG + chr4_2:73870682-73870701 Msa0621650:CDS 40.0%
!! AACCAGTTCTGAAATAGACG+CGG - chr4_2:73870731-73870750 None:intergenic 40.0%
CAAACAACTTGATGTGCACA+TGG - chr4_2:73870850-73870869 None:intergenic 40.0%
ATGCATTCAGATCTTGGATC+TGG + chr4_2:73871038-73871057 Msa0621650:three_prime_UTR 40.0%
TGTGCTATGTGTTGAGCTTG+GGG - chr4_2:73866655-73866674 None:intergenic 45.0%
TAATTGATCCGTGCATCCCA+CGG - chr4_2:73867423-73867442 None:intergenic 45.0%
TTGCAGCTAAATGGCATCAC+TGG + chr4_2:73867795-73867814 Msa0621650:intron 45.0%
TTACTTGACTCCCCTAAAGG+TGG - chr4_2:73867904-73867923 None:intergenic 45.0%
CTCTTACTTGACTCCCCTAA+AGG - chr4_2:73867907-73867926 None:intergenic 45.0%
! TGTGACTTGCCCAAAACATG+GGG - chr4_2:73867992-73868011 None:intergenic 45.0%
! CTGTGACTTGCCCAAAACAT+GGG - chr4_2:73867993-73868012 None:intergenic 45.0%
TGACCTCAGATGAAACTGCA+GGG - chr4_2:73868083-73868102 None:intergenic 45.0%
CTTCTAATTCCTCCTTAGGC+TGG - chr4_2:73868113-73868132 None:intergenic 45.0%
GCCTAAGGAGGAATTAGAAG+TGG + chr4_2:73868113-73868132 Msa0621650:CDS 45.0%
TTAGAAGTGGTTGAGTCCCA+TGG + chr4_2:73868126-73868145 Msa0621650:CDS 45.0%
TTGCATCCATAACAGATGCC+AGG - chr4_2:73868291-73868310 None:intergenic 45.0%
TCAGTTTCGGAGATGCAATG+TGG + chr4_2:73868469-73868488 Msa0621650:CDS 45.0%
TCACATGCTTGTGCAATGGA+TGG + chr4_2:73868519-73868538 Msa0621650:CDS 45.0%
AGTTTATGCAGCGATGGCAT+TGG + chr4_2:73868555-73868574 Msa0621650:CDS 45.0%
CCTGGGGGAATTGAATTACA+AGG - chr4_2:73868657-73868676 None:intergenic 45.0%
CCTTGTAATTCAATTCCCCC+AGG + chr4_2:73868654-73868673 Msa0621650:CDS 45.0%
TTTCTCAGATTGAGTGCCTG+GGG - chr4_2:73868673-73868692 None:intergenic 45.0%
GATTTCTCAGATTGAGTGCC+TGG - chr4_2:73868675-73868694 None:intergenic 45.0%
TTGTAGCATGGGAGATTGAG+AGG - chr4_2:73868799-73868818 None:intergenic 45.0%
! CCCGCATTTTTCTTGTAGCA+TGG - chr4_2:73868811-73868830 None:intergenic 45.0%
TATCGGACATACGGGTCTAA+AGG + chr4_2:73868909-73868928 Msa0621650:CDS 45.0%
ATCGGACATACGGGTCTAAA+GGG + chr4_2:73868910-73868929 Msa0621650:CDS 45.0%
TCTAAAGGGTCTTCAAGAGG+AGG + chr4_2:73868924-73868943 Msa0621650:CDS 45.0%
AGGCTAAATGTTGGGGTAGA+TGG - chr4_2:73869113-73869132 None:intergenic 45.0%
ATCTACCCCAACATTTAGCC+TGG + chr4_2:73869112-73869131 Msa0621650:CDS 45.0%
! GTAGCTGATAATGCATCTGG+GGG - chr4_2:73869218-73869237 None:intergenic 45.0%
! TGATTCTGAAGATGGTTCCG+AGG + chr4_2:73869387-73869406 Msa0621650:CDS 45.0%
! GATTCTGAAGATGGTTCCGA+GGG + chr4_2:73869388-73869407 Msa0621650:CDS 45.0%
GACAGAGTGGGCAAATCAAA+AGG + chr4_2:73869442-73869461 Msa0621650:CDS 45.0%
GGCAAATCAAAAGGTGATGC+AGG + chr4_2:73869451-73869470 Msa0621650:CDS 45.0%
AGGCTGAGCTTAAGACACTA+AGG + chr4_2:73869498-73869517 Msa0621650:CDS 45.0%
GAAAATGCCTTGGGTAAAGC+AGG + chr4_2:73869602-73869621 Msa0621650:CDS 45.0%
AGATGGAGAATGCTGCATTG+AGG + chr4_2:73869666-73869685 Msa0621650:CDS 45.0%
TGCTGCATTGAGGAAAGAGA+TGG + chr4_2:73869676-73869695 Msa0621650:CDS 45.0%
TGCATTGAGGAAAGAGATGG+AGG + chr4_2:73869679-73869698 Msa0621650:CDS 45.0%
! AGCTGTTGTTTGTTCTCACG+GGG + chr4_2:73870091-73870110 Msa0621650:intron 45.0%
CATTACCCACCCATTCAACA+AGG - chr4_2:73870279-73870298 None:intergenic 45.0%
CAATTTGATCCTCTCCTCCT+TGG - chr4_2:73870527-73870546 None:intergenic 45.0%
CAAGGAGGAGAGGATCAAAT+TGG + chr4_2:73870525-73870544 Msa0621650:CDS 45.0%
GGAGAGGATCAAATTGGAAG+CGG + chr4_2:73870531-73870550 Msa0621650:CDS 45.0%
GATCAAATTGGAAGCGGAGA+AGG + chr4_2:73870537-73870556 Msa0621650:CDS 45.0%
TTTCGTGTCCTTGAAGCTAC+CGG - chr4_2:73870680-73870699 None:intergenic 45.0%
AAGGACACGAAAAAGGGTTC+TGG + chr4_2:73870688-73870707 Msa0621650:CDS 45.0%
!! TTCTGAAATAGACGCGGTGT+GGG - chr4_2:73870725-73870744 None:intergenic 45.0%
AACTTCTCTATGAACGGAGG+AGG + chr4_2:73870763-73870782 Msa0621650:CDS 45.0%
CAACAGACATCTCTTCCGAA+AGG - chr4_2:73870819-73870838 None:intergenic 45.0%
AATGAGCTCCATGAGAAGCA+GGG + chr4_2:73870880-73870899 Msa0621650:CDS 45.0%
TGCAAGATTGTCCTTCTTGC+AGG + chr4_2:73870905-73870924 Msa0621650:CDS 45.0%
ACAGAAATTCGCTCCTGGAT+GGG - chr4_2:73870934-73870953 None:intergenic 45.0%
ATTATTCTATGGGAGTCCCG+AGG - chr4_2:73871020-73871039 None:intergenic 45.0%
! ATTTGCTAGTGCGTATGCCG+TGG + chr4_2:73867403-73867422 Msa0621650:intron 50.0%
TTTGCTAGTGCGTATGCCGT+GGG + chr4_2:73867404-73867423 Msa0621650:intron 50.0%
! ATGGCATCACTGGTTGTCAG+TGG + chr4_2:73867805-73867824 Msa0621650:CDS 50.0%
TTCAGGGCTGATCCACCTTT+AGG + chr4_2:73867889-73867908 Msa0621650:CDS 50.0%
TCAGGGCTGATCCACCTTTA+GGG + chr4_2:73867890-73867909 Msa0621650:CDS 50.0%
! GCTGTGACTTGCCCAAAACA+TGG - chr4_2:73867994-73868013 None:intergenic 50.0%
GACTTGTGCACTGTTAGCCA+CGG + chr4_2:73868018-73868037 Msa0621650:CDS 50.0%
GTTAGCCACGGTTGTTCTGA+TGG + chr4_2:73868030-73868049 Msa0621650:CDS 50.0%
TCAAGCCATCAGAACAACCG+TGG - chr4_2:73868038-73868057 None:intergenic 50.0%
CTGCAGGGCTAGATAATCCA+AGG - chr4_2:73868068-73868087 None:intergenic 50.0%
TAGCCCTGCAGTTTCATCTG+AGG + chr4_2:73868077-73868096 Msa0621650:CDS 50.0%
CTGACCTCAGATGAAACTGC+AGG - chr4_2:73868084-73868103 None:intergenic 50.0%
! CTGCAGTTTCATCTGAGGTC+AGG + chr4_2:73868082-73868101 Msa0621650:CDS 50.0%
ATCACTCCAATCAGCGCCAT+GGG - chr4_2:73868145-73868164 None:intergenic 50.0%
CGTGTCACATGCTTGTGCAA+TGG + chr4_2:73868515-73868534 Msa0621650:CDS 50.0%
TTCTCAGATTGAGTGCCTGG+GGG - chr4_2:73868672-73868691 None:intergenic 50.0%
! TCAAATTCGCGGTGGACTCT+TGG + chr4_2:73868713-73868732 Msa0621650:CDS 50.0%
CATGGGAGATTGAGAGGTTC+CGG - chr4_2:73868793-73868812 None:intergenic 50.0%
TCCATTCGAGTAGCACCAAG+AGG + chr4_2:73868844-73868863 Msa0621650:CDS 50.0%
CCATTCGAGTAGCACCAAGA+GGG + chr4_2:73868845-73868864 Msa0621650:CDS 50.0%
TCGTCAGAAGTCAATCCACG+TGG + chr4_2:73868878-73868897 Msa0621650:CDS 50.0%
GGGTCTAAAGGGTCTTCAAG+AGG + chr4_2:73868921-73868940 Msa0621650:CDS 50.0%
TCAAGAGGAGGAAAGCTGAG+TGG + chr4_2:73868936-73868955 Msa0621650:CDS 50.0%
GGAAAGCTGAGTGGTTTGAG+TGG + chr4_2:73868945-73868964 Msa0621650:CDS 50.0%
! GGACTACTGACTGCTGGTAT+AGG - chr4_2:73869197-73869216 None:intergenic 50.0%
TTGGGGCAACCATTGTCTCA+TGG - chr4_2:73869351-73869370 None:intergenic 50.0%
TGAAGATGGTTCCGAGGGTT+CGG + chr4_2:73869393-73869412 Msa0621650:CDS 50.0%
! TCGTCGACTGAGTAAGGACA+AGG + chr4_2:73869478-73869497 Msa0621650:CDS 50.0%
AATGCCTTGGGTAAAGCAGG+TGG + chr4_2:73869605-73869624 Msa0621650:CDS 50.0%
TTGTCCACCTGCTTTACCCA+AGG - chr4_2:73869612-73869631 None:intergenic 50.0%
AAAGCAAGCCGAAGTGCAAG+CGG + chr4_2:73869865-73869884 Msa0621650:CDS 50.0%
GAATTGCTCCGCTTGCACTT+CGG - chr4_2:73869876-73869895 None:intergenic 50.0%
! GAACCGCATGGCAAAGCATT+TGG - chr4_2:73869924-73869943 None:intergenic 50.0%
TCTGGTGAAGATGAACCGCA+TGG - chr4_2:73869936-73869955 None:intergenic 50.0%
! GCTGTTGTTTGTTCTCACGG+GGG + chr4_2:73870092-73870111 Msa0621650:intron 50.0%
! CTGTTGTTTGTTCTCACGGG+GGG + chr4_2:73870093-73870112 Msa0621650:intron 50.0%
! ATACGTGTCACTGCGTGTGT+CGG - chr4_2:73870217-73870236 None:intergenic 50.0%
GGAATCGATGGAAGCTATGC+CGG + chr4_2:73870658-73870677 Msa0621650:CDS 50.0%
AAGCTATGCCGGTAGCTTCA+AGG + chr4_2:73870669-73870688 Msa0621650:CDS 50.0%
! TCTGAAATAGACGCGGTGTG+GGG - chr4_2:73870724-73870743 None:intergenic 50.0%
!! GTTCTGAAATAGACGCGGTG+TGG - chr4_2:73870726-73870745 None:intergenic 50.0%
CACCGCGTCTATTTCAGAAC+TGG + chr4_2:73870726-73870745 Msa0621650:CDS 50.0%
! TGAACGGAGGAGGTGTGAAA+CGG + chr4_2:73870773-73870792 Msa0621650:CDS 50.0%
! AGGAGGTGTGAAACGGGAAA+GGG + chr4_2:73870780-73870799 Msa0621650:CDS 50.0%
GGAGTGTGTGATGTGCCTTT+CGG + chr4_2:73870801-73870820 Msa0621650:CDS 50.0%
CAATGAGCTCCATGAGAAGC+AGG + chr4_2:73870879-73870898 Msa0621650:CDS 50.0%
CTTGCATTCCCTGCTTCTCA+TGG - chr4_2:73870891-73870910 None:intergenic 50.0%
CAGAAATTCGCTCCTGGATG+GGG - chr4_2:73870933-73870952 None:intergenic 50.0%
CACAGAAATTCGCTCCTGGA+TGG - chr4_2:73870935-73870954 None:intergenic 50.0%
AACGCACAGAAATTCGCTCC+TGG - chr4_2:73870939-73870958 None:intergenic 50.0%
GCTATGTGTTGAGCTTGGGG+AGG - chr4_2:73866652-73866671 None:intergenic 55.0%
CAGGGCTGATCCACCTTTAG+GGG + chr4_2:73867891-73867910 Msa0621650:CDS 55.0%
GATGGCTTGAAGCTCGACCT+TGG + chr4_2:73868048-73868067 Msa0621650:CDS 55.0%
!! TTGAGTCCCATGGCGCTGAT+TGG + chr4_2:73868136-73868155 Msa0621650:CDS 55.0%
GATCACTCCAATCAGCGCCA+TGG - chr4_2:73868146-73868165 None:intergenic 55.0%
!! TGCAGCGATGGCATTGGTGA+TGG + chr4_2:73868561-73868580 Msa0621650:CDS 55.0%
!! CCCTCTTGGTGCTACTCGAA+TGG - chr4_2:73868848-73868867 None:intergenic 55.0%
GAAGATGGTTCCGAGGGTTC+GGG + chr4_2:73869394-73869413 Msa0621650:CDS 55.0%
! TTTTGCAGCTCCCGAACCCT+CGG - chr4_2:73869407-73869426 None:intergenic 55.0%
GGGTAAAGCAGGTGGACAAG+TGG + chr4_2:73869613-73869632 Msa0621650:CDS 55.0%
TGCAGTACGGAAGCTCGAGA+TGG + chr4_2:73869649-73869668 Msa0621650:CDS 55.0%
GGAGGCTGCTAAGTTACGTG+CGG + chr4_2:73869697-73869716 Msa0621650:CDS 55.0%
AGTGCAAGCGGAGCAATTCG+AGG + chr4_2:73869877-73869896 Msa0621650:CDS 55.0%
TTTGTTCTCACGGGGGGCTT+CGG + chr4_2:73870099-73870118 Msa0621650:intron 55.0%
TGAGGAGTCGGCTAGAACCA+AGG + chr4_2:73870507-73870526 Msa0621650:CDS 55.0%
! GAACGGAGGAGGTGTGAAAC+GGG + chr4_2:73870774-73870793 Msa0621650:CDS 55.0%
! GAGGAGGTGTGAAACGGGAA+AGG + chr4_2:73870779-73870798 Msa0621650:CDS 55.0%
TGCGTATGCCGTGGGATGCA+CGG + chr4_2:73867412-73867431 Msa0621650:intron 60.0%
GGTCAGGCTTAGCCAGCCTA+AGG + chr4_2:73868098-73868117 Msa0621650:CDS 60.0%
CAGGCTTAGCCAGCCTAAGG+AGG + chr4_2:73868101-73868120 Msa0621650:CDS 60.0%
! TCTGGGGGACTACTGACTGC+TGG - chr4_2:73869203-73869222 None:intergenic 60.0%
! GGCTGCTCGTCGACTGAGTA+AGG + chr4_2:73869472-73869491 Msa0621650:CDS 60.0%
ATCACAGGCTAAACGGCGGC+AGG + chr4_2:73870420-73870439 Msa0621650:intron 60.0%
GGAGTCGGCTAGAACCAAGG+AGG + chr4_2:73870510-73870529 Msa0621650:CDS 60.0%
CGGCTAGAACCAAGGAGGAG+AGG + chr4_2:73870515-73870534 Msa0621650:CDS 60.0%
TGGATGGGGCTCCTGCAAGA+AGG - chr4_2:73870919-73870938 None:intergenic 60.0%
TTCTTGCAGGAGCCCCATCC+AGG + chr4_2:73870918-73870937 Msa0621650:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 73866623 73871114 73866623 ID=Msa0621650;Name=Msa0621650
chr4_2 mRNA 73866623 73871114 73866623 ID=Msa0621650-mRNA-1;Parent=Msa0621650;Name=Msa0621650-mRNA-1;_AED=0.07;_eAED=0.07;_QI=234|1|1|1|0.33|0.25|4|142|879
chr4_2 exon 73866623 73866813 73866623 ID=Msa0621650-mRNA-1:exon:22166;Parent=Msa0621650-mRNA-1
chr4_2 exon 73867092 73867130 73867092 ID=Msa0621650-mRNA-1:exon:22167;Parent=Msa0621650-mRNA-1
chr4_2 exon 73867801 73869898 73867801 ID=Msa0621650-mRNA-1:exon:22168;Parent=Msa0621650-mRNA-1
chr4_2 exon 73870427 73871114 73870427 ID=Msa0621650-mRNA-1:exon:22169;Parent=Msa0621650-mRNA-1
chr4_2 five_prime_UTR 73866623 73866813 73866623 ID=Msa0621650-mRNA-1:five_prime_utr;Parent=Msa0621650-mRNA-1
chr4_2 five_prime_UTR 73867092 73867130 73867092 ID=Msa0621650-mRNA-1:five_prime_utr;Parent=Msa0621650-mRNA-1
chr4_2 five_prime_UTR 73867801 73867804 73867801 ID=Msa0621650-mRNA-1:five_prime_utr;Parent=Msa0621650-mRNA-1
chr4_2 CDS 73867805 73869898 73867805 ID=Msa0621650-mRNA-1:cds;Parent=Msa0621650-mRNA-1
chr4_2 CDS 73870427 73870972 73870427 ID=Msa0621650-mRNA-1:cds;Parent=Msa0621650-mRNA-1
chr4_2 three_prime_UTR 73870973 73871114 73870973 ID=Msa0621650-mRNA-1:three_prime_utr;Parent=Msa0621650-mRNA-1
Gene Sequence

>Msa0621650

ATGGCATCACTGGTTGTCAGTGGTAACAGTCAAATGTCTTCTTCGGTTTCTGTTCAAGAAAAAGGAAGTAGAAATAAGAGAAAATTCAGGGCTGATCCACCTTTAGGGGAGTCAAGTAAGAGTATATCTTCACTTCAACATGAGAGCCTAAGTTATGAATTCTCTGCTGAAAAAGTCGAAATTACCCCATGTTTTGGGCAAGTCACAGCTTCTGACTTGTGCACTGTTAGCCACGGTTGTTCTGATGGCTTGAAGCTCGACCTTGGATTATCTAGCCCTGCAGTTTCATCTGAGGTCAGGCTTAGCCAGCCTAAGGAGGAATTAGAAGTGGTTGAGTCCCATGGCGCTGATTGGAGTGATCACACAGAAACTCAGTTGCAAGAACTTGTCTTGAGCAATTTGCAAACCATTTTCAAGAGTGCAATAAAAAAAATTGTTGCTTGTGGCTACACTGAAGATGTTGCTACAAAGGCTATGTTAAGACCTGGCATCTGTTATGGATGCAAAGACACTGTGTCTAATATAGTTGACAACACTTTAGCTCTTTTGAGAAACGGTCAAGAGTTTGATCCTTCACGAGAGCACTATTTTAAAGATTTAGCCGAGCTTCAGAATTACATCTTGGCCGAATTGGTTTGCGTTCTTCAAGAGGTTAGGCCATTTTTCAGTTTCGGAGATGCAATGTGGTGTTTGTTAATCAGTGACATGAACGTGTCACATGCTTGTGCAATGGATGGTGACCCTTTAAGTAGTTTATGCAGCGATGGCATTGGTGATGGCTCTTCTTCTGTCCAGACTGAATCACAATCAAAAGCTGAAATAAAAAGTTCGGAATTGAGTCTTCCAAGTCCTTGTAATTCAATTCCCCCAGGCACTCAATCTGAGAAATCCGTTGTGGCAGAAAATTCTCAAATTCGCGGTGGACTCTTGGAAAAACAGAGTGCTAATTCTGGATGTCATTCTGTTGACAAGTCATCTAATGCTTCCGGAACCTCTCAATCTCCCATGCTACAAGAAAAATGCGGGATTGTTAGAAAGGTCCATTCGAGTAGCACCAAGAGGGAGTACATTTTTCGTCAGAAGTCAATCCACGTGGAAAAAAGTTATCGGACATACGGGTCTAAAGGGTCTTCAAGAGGAGGAAAGCTGAGTGGTTTGAGTGGTTTAATCCTGGATAAAAAACTTAAATCAGTATCTGAATCTACTGCCATAAACTTAAAGAGTGCTTCTATAAACATAAGCAAGGCAGTGGGAATTGATGTCACTCAAAACAATCATAACACCCATTTCTCATCTAATAATGGACCATCTACCCCAACATTTAGCCTGGATTCTAGTGATACTATTTCTCGTGCAGCTGATTCTTCATCTTCAGAACATGAAGCAAATCCTATACCAGCAGTCAGTAGTCCCCCAGATGCATTATCAGCTACAGACACTGACCTTTCTCTTTCATTGTCTTCAAAAGGCAATTCTTCTATAGCCCCTATCTGCTGTAATAACAAGTCACATAGTAGTTGTGTGGGGATACCATATGACAAGTCCATGAGACAATGGTTGCCCCAAGATAGAAAAGATGAGTTGATTCTGAAGATGGTTCCGAGGGTTCGGGAGCTGCAAAATGAACTTCAAGAATGGACAGAGTGGGCAAATCAAAAGGTGATGCAGGCTGCTCGTCGACTGAGTAAGGACAAGGCTGAGCTTAAGACACTAAGGCAAGAGAAAGAAGAAGTTGAACGACTTAAAAAGGAGAAACAATGCCTTGAAGAAAACACCATGAAAAAGCTTTCTGAGATGGAAAATGCCTTGGGTAAAGCAGGTGGACAAGTGGAACGAGCTAATACTGCAGTACGGAAGCTCGAGATGGAGAATGCTGCATTGAGGAAAGAGATGGAGGCTGCTAAGTTACGTGCGGAAGAATCAGCAACAAACTTTCAAGAGGTCTCTAAGAGAGAAAAGAAGACCCAAATGAAGTTTCAATCATGGGAGAATCAGAAATCGTTGTTACAGGAAGAGCTAATGACTGAAAAAAACAAATTGGCACAGATATCGAAGGAATCAAAGCAAGCCGAAGTGCAAGCGGAGCAATTCGAGGCTAAACGGCGGCAGGCAGCAAAGAAAACAGAAGAACTTCTCTCCATGGTCAGTTCTATACGAAAAGAAAGAGAACAAATTGAGGAGTCGGCTAGAACCAAGGAGGAGAGGATCAAATTGGAAGCGGAGAAGGAGCTGCGAAGATACAAAGACGACATCCAGAAACTTGAAAAAGAAATTGCACAAATAAGACAGAAGTCCGACTCTTCAAAAATTGCTGCACTAAAAAGAGGAATCGATGGAAGCTATGCCGGTAGCTTCAAGGACACGAAAAAGGGTTCTGGTTTTGAAGAACCCCACACCGCGTCTATTTCAGAACTGGTTCAAAAGTTAAATAACTTCTCTATGAACGGAGGAGGTGTGAAACGGGAAAGGGAGTGTGTGATGTGCCTTTCGGAAGAGATGTCTGTTGTATTTCTTCCATGTGCACATCAAGTTGTTTGCACAAAGTGCAATGAGCTCCATGAGAAGCAGGGAATGCAAGATTGTCCTTCTTGCAGGAGCCCCATCCAGGAGCGAATTTCTGTGCGTTATGCGCGTACATAA
Protein sequence

>Msa0621650

MASLVVSGNSQMSSSVSVQEKGSRNKRKFRADPPLGESSKSISSLQHESLSYEFSAEKVEITPCFGQVTASDLCTVSHGCSDGLKLDLGLSSPAVSSEVRLSQPKEELEVVESHGADWSDHTETQLQELVLSNLQTIFKSAIKKIVACGYTEDVATKAMLRPGICYGCKDTVSNIVDNTLALLRNGQEFDPSREHYFKDLAELQNYILAELVCVLQEVRPFFSFGDAMWCLLISDMNVSHACAMDGDPLSSLCSDGIGDGSSSVQTESQSKAEIKSSELSLPSPCNSIPPGTQSEKSVVAENSQIRGGLLEKQSANSGCHSVDKSSNASGTSQSPMLQEKCGIVRKVHSSSTKREYIFRQKSIHVEKSYRTYGSKGSSRGGKLSGLSGLILDKKLKSVSESTAINLKSASINISKAVGIDVTQNNHNTHFSSNNGPSTPTFSLDSSDTISRAADSSSSEHEANPIPAVSSPPDALSATDTDLSLSLSSKGNSSIAPICCNNKSHSSCVGIPYDKSMRQWLPQDRKDELILKMVPRVRELQNELQEWTEWANQKVMQAARRLSKDKAELKTLRQEKEEVERLKKEKQCLEENTMKKLSEMENALGKAGGQVERANTAVRKLEMENAALRKEMEAAKLRAEESATNFQEVSKREKKTQMKFQSWENQKSLLQEELMTEKNKLAQISKESKQAEVQAEQFEAKRRQAAKKTEELLSMVSSIRKEREQIEESARTKEERIKLEAEKELRRYKDDIQKLEKEIAQIRQKSDSSKIAALKRGIDGSYAGSFKDTKKGSGFEEPHTASISELVQKLNNFSMNGGGVKRERECVMCLSEEMSVVFLPCAHQVVCTKCNELHEKQGMQDCPSCRSPIQERISVRYART*