AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0733890

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0733890 MtrunA17_Chr5g0429501 100.000 161 0 0 1 161 1 161 1.27e-118 331
Msa0733890 MtrunA17_Chr3g0104001 46.012 163 70 5 4 160 4 154 2.30e-41 135
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0733890 AT5G58920.1 63.636 165 52 2 1 160 1 162 2.33e-74 220

Find 68 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGGAGGAGTTTATTAAAA+AGG 0.222603 5_1:-47415265 Msa0733890:CDS
TTTAACAAATGTACCTGTTT+TGG 0.232762 5_1:+47415636 None:intergenic
GCTTCCTCGTCTCCTGGATA+TGG 0.282324 5_1:+47415288 None:intergenic
AGTGCTTCTGCAAAGGAAAA+TGG 0.305320 5_1:+47415939 None:intergenic
TGAAGTTATTGGTGAGCTTC+AGG 0.305838 5_1:-47415109 Msa0733890:CDS
TCATTCTCCACCATCCCTTT+AGG 0.312626 5_1:+47415216 None:intergenic
GTGAAGTGAAGTAATGAATA+TGG 0.338877 5_1:+47415700 None:intergenic
AGGGATGGTGGAGAATGATA+TGG 0.349759 5_1:-47415211 Msa0733890:CDS
TTCCAGAAGTAGTAGTTATT+CGG 0.356863 5_1:+47415459 None:intergenic
GACCGAATAACTACTACTTC+TGG 0.360457 5_1:-47415461 Msa0733890:CDS
TGATTATAAGAAGGAGCTTA+AGG 0.365177 5_1:-47415181 Msa0733890:CDS
TCCAGCTCTTTCTCTTCCTT+CGG 0.366817 5_1:+47415336 None:intergenic
AGCAAGAGGCTCAGAAGCTT+TGG 0.366979 5_1:-47415146 Msa0733890:CDS
TATGTGTATATCTAAGTAAT+GGG 0.370700 5_1:-47414848 Msa0733890:three_prime_UTR
AGGCTCAGAAGCTTTGGTTT+AGG 0.390308 5_1:-47415140 Msa0733890:CDS
CTACTACTTCTGGAAACAAA+TGG 0.394820 5_1:-47415451 Msa0733890:CDS
TACTACTTCTGGAAACAAAT+GGG 0.395334 5_1:-47415450 Msa0733890:CDS
TGTTAAGCGCACCTGGGATT+GGG 0.396887 5_1:+47415870 None:intergenic
GGGATGATTGGAACAACTAT+TGG 0.398836 5_1:-47415240 Msa0733890:CDS
TCCATAGCTTCCTCGTCTCC+TGG 0.401637 5_1:+47415282 None:intergenic
TCTTTCTCTTCCTTCGGCAT+TGG 0.403880 5_1:+47415342 None:intergenic
AGGATGAGGAATGAAGTTAT+TGG 0.417045 5_1:-47415120 Msa0733890:CDS
TAAGTAATGGGATTGTTTAG+AGG 0.419513 5_1:-47414836 Msa0733890:three_prime_UTR
GAAACAAATGGGGAAGGCTT+TGG 0.427981 5_1:-47415439 Msa0733890:CDS
TTGAATCGAATCCTTAAAGT+TGG 0.431687 5_1:-47415055 Msa0733890:three_prime_UTR
TTTCTAAAGAAAATAGGGTT+GGG 0.438410 5_1:-47415483 Msa0733890:CDS
TGAATCGAATCCTTAAAGTT+GGG 0.445885 5_1:-47415054 Msa0733890:three_prime_UTR
TTGTTAAGCGCACCTGGGAT+TGG 0.454395 5_1:+47415869 None:intergenic
TTATGTGTATATCTAAGTAA+TGG 0.455248 5_1:-47414849 Msa0733890:three_prime_UTR
GAAACACCGCAACCCAAAAC+AGG 0.459747 5_1:-47415649 Msa0733890:intron
AGAAGCTTTGGTTTAGGATG+AGG 0.464134 5_1:-47415134 Msa0733890:CDS
GGAACAACTATTGGACCTAA+AGG 0.464559 5_1:-47415231 Msa0733890:CDS
TTCAACGAGACGTCGCCATT+GGG 0.472078 5_1:-47415381 Msa0733890:CDS
TTTCACTCATTCCCAATCCC+AGG 0.473777 5_1:-47415881 Msa0733890:intron
ATTCAACGAGACGTCGCCAT+TGG 0.476874 5_1:-47415382 Msa0733890:CDS
GAACAACTATTGGACCTAAA+GGG 0.484376 5_1:-47415230 Msa0733890:CDS
CGGCGTGAGTTTCCATATCC+AGG 0.484424 5_1:-47415300 Msa0733890:CDS
GGATAAGAAGTTTGAGCAAG+AGG 0.490071 5_1:-47415160 Msa0733890:CDS
TTAAAATACTACATTTGTGT+TGG 0.495464 5_1:-47415029 Msa0733890:three_prime_UTR
TATTGGTGAGCTTCAGGAGA+AGG 0.502673 5_1:-47415103 Msa0733890:CDS
TCCAGGAGACGAGGAAGCTA+TGG 0.505414 5_1:-47415283 Msa0733890:CDS
GGCGTGCACTTATGCAGTGA+TGG 0.519711 5_1:-47415418 Msa0733890:CDS
AAGTAATGGGATTGTTTAGA+GGG 0.520693 5_1:-47414835 Msa0733890:three_prime_UTR
AAGCACAAAGAAGCCAAATC+CGG 0.521029 5_1:+47414874 None:intergenic
AGACGTCGCCATTGGGTTGG+TGG 0.529186 5_1:-47415374 Msa0733890:CDS
GAATCGAATCCTTAAAGTTG+GGG 0.531721 5_1:-47415053 Msa0733890:three_prime_UTR
GGATGAGAGTGATTATAAGA+AGG 0.532632 5_1:-47415190 Msa0733890:CDS
GCCGAAGGAAGAGAAAGAGC+TGG 0.546418 5_1:-47415337 Msa0733890:CDS
ATTGGTGAGCTTCAGGAGAA+GGG 0.553628 5_1:-47415102 Msa0733890:CDS
CAAAGAAGCCAAATCCGGTA+AGG 0.566719 5_1:+47414879 None:intergenic
CTCGACAAGTGCTTCTGCAA+AGG 0.569134 5_1:+47415932 None:intergenic
AGGAGACGAGGAAGCTATGG+AGG 0.571758 5_1:-47415280 Msa0733890:CDS
GCGTGCACTTATGCAGTGAT+GGG 0.574227 5_1:-47415417 Msa0733890:CDS
ACGAGACGTCGCCATTGGGT+TGG 0.576055 5_1:-47415377 Msa0733890:CDS
AGCTGGAACATTTGTATCAG+CGG 0.579371 5_1:-47415320 Msa0733890:CDS
GACGTTGAAGCCAATGCCGA+AGG 0.592265 5_1:-47415352 Msa0733890:CDS
GTTTCGAAGAAGCGTTTACG+AGG 0.608927 5_1:+47415667 None:intergenic
AACTATTGGACCTAAAGGGA+TGG 0.616902 5_1:-47415226 Msa0733890:CDS
CACTTATGCAGTGATGGGTG+CGG 0.618073 5_1:-47415412 Msa0733890:CDS
TAATGAATATGGAACGATGA+TGG 0.624807 5_1:+47415711 None:intergenic
TATTGGACCTAAAGGGATGG+TGG 0.625420 5_1:-47415223 Msa0733890:CDS
TTCAACGTCCACCAACCCAA+TGG 0.629360 5_1:+47415366 None:intergenic
CTTCTGGAAACAAATGGGGA+AGG 0.632900 5_1:-47415445 Msa0733890:CDS
ACTACTTCTGGAAACAAATG+GGG 0.649071 5_1:-47415449 Msa0733890:CDS
GTTTCCATATCCAGGAGACG+AGG 0.689791 5_1:-47415292 Msa0733890:CDS
AACAGCATGTGCGATCCTCG+TGG 0.694988 5_1:-47415983 Msa0733890:five_prime_UTR
ATGCAGTGATGGGTGCGGCG+TGG 0.699663 5_1:-47415407 Msa0733890:CDS
ATAATATGAAGATTGCCACG+AGG 0.795185 5_1:+47415968 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATAAAATGTAAAAAATTAT+TGG + chr5_1:47415260-47415279 None:intergenic 10.0%
!!! TATTTTCTTTAGAAAATATT+GGG + chr5_1:47415319-47415338 None:intergenic 10.0%
!!! AATATTTTCTAAAGAAAATA+GGG - chr5_1:47415319-47415338 Msa0733890:CDS 10.0%
!! CATAAAATGTAAAAAATTAT+TGG + chr5_1:47415260-47415279 None:intergenic 10.0%
!!! TATTTTCTTTAGAAAATATT+GGG + chr5_1:47415319-47415338 None:intergenic 10.0%
!!! AATATTTTCTAAAGAAAATA+GGG - chr5_1:47415319-47415338 Msa0733890:CDS 10.0%
!!! CTATTTTCTTTAGAAAATAT+TGG + chr5_1:47415320-47415339 None:intergenic 15.0%
!!! CAATATTTTCTAAAGAAAAT+AGG - chr5_1:47415318-47415337 Msa0733890:CDS 15.0%
!!! GTTATGTTTTTAATAAATCA+TGG - chr5_1:47415820-47415839 Msa0733890:intron 15.0%
!!! CTATTTTCTTTAGAAAATAT+TGG + chr5_1:47415320-47415339 None:intergenic 15.0%
!!! CAATATTTTCTAAAGAAAAT+AGG - chr5_1:47415318-47415337 Msa0733890:CDS 15.0%
!!! GTTATGTTTTTAATAAATCA+TGG - chr5_1:47415820-47415839 Msa0733890:intron 15.0%
!!! TAAGATTCAATTTGAAGTTA+GGG + chr5_1:47415058-47415077 None:intergenic 20.0%
!!! ATAAGATTCAATTTGAAGTT+AGG + chr5_1:47415059-47415078 None:intergenic 20.0%
!! TTAAAATACTACATTTGTGT+TGG - chr5_1:47415778-47415797 Msa0733890:intron 20.0%
!! TTATGTGTATATCTAAGTAA+TGG - chr5_1:47415958-47415977 Msa0733890:five_prime_UTR 20.0%
!! TATGTGTATATCTAAGTAAT+GGG - chr5_1:47415959-47415978 Msa0733890:five_prime_UTR 20.0%
!!! TAAGATTCAATTTGAAGTTA+GGG + chr5_1:47415058-47415077 None:intergenic 20.0%
!!! ATAAGATTCAATTTGAAGTT+AGG + chr5_1:47415059-47415078 None:intergenic 20.0%
!! TTAAAATACTACATTTGTGT+TGG - chr5_1:47415778-47415797 Msa0733890:intron 20.0%
!! TTATGTGTATATCTAAGTAA+TGG - chr5_1:47415958-47415977 Msa0733890:five_prime_UTR 20.0%
!! TATGTGTATATCTAAGTAAT+GGG - chr5_1:47415959-47415978 Msa0733890:five_prime_UTR 20.0%
!! TTAACAAATGTACCTGTTTT+GGG + chr5_1:47415173-47415192 None:intergenic 25.0%
!! TTTAACAAATGTACCTGTTT+TGG + chr5_1:47415174-47415193 None:intergenic 25.0%
!! AATTTTATGCATCTTAATGC+AGG - chr5_1:47415282-47415301 Msa0733890:CDS 25.0%
!!! TTTTCTAAAGAAAATAGGGT+TGG - chr5_1:47415323-47415342 Msa0733890:CDS 25.0%
!! TTTCTAAAGAAAATAGGGTT+GGG - chr5_1:47415324-47415343 Msa0733890:CDS 25.0%
! TATGGAGGAGTTTATTAAAA+AGG - chr5_1:47415542-47415561 Msa0733890:intron 25.0%
! ATGGAGGAGTTTATTAAAAA+GGG - chr5_1:47415543-47415562 Msa0733890:intron 25.0%
!!! TATTTTAATCCCCAACTTTA+AGG + chr5_1:47415766-47415785 None:intergenic 25.0%
!!! ATCACATTTTTTTTCCTTAC+CGG - chr5_1:47415914-47415933 Msa0733890:five_prime_UTR 25.0%
!! TTAACAAATGTACCTGTTTT+GGG + chr5_1:47415173-47415192 None:intergenic 25.0%
!! TTTAACAAATGTACCTGTTT+TGG + chr5_1:47415174-47415193 None:intergenic 25.0%
!! AATTTTATGCATCTTAATGC+AGG - chr5_1:47415282-47415301 Msa0733890:CDS 25.0%
!!! TTTTCTAAAGAAAATAGGGT+TGG - chr5_1:47415323-47415342 Msa0733890:CDS 25.0%
!! TTTCTAAAGAAAATAGGGTT+GGG - chr5_1:47415324-47415343 Msa0733890:CDS 25.0%
! TATGGAGGAGTTTATTAAAA+AGG - chr5_1:47415542-47415561 Msa0733890:intron 25.0%
! ATGGAGGAGTTTATTAAAAA+GGG - chr5_1:47415543-47415562 Msa0733890:intron 25.0%
!!! TATTTTAATCCCCAACTTTA+AGG + chr5_1:47415766-47415785 None:intergenic 25.0%
!!! ATCACATTTTTTTTCCTTAC+CGG - chr5_1:47415914-47415933 Msa0733890:five_prime_UTR 25.0%
TTGGGAATGAGTGAAAAAAA+AGG + chr5_1:47414922-47414941 None:intergenic 30.0%
TAATGAATATGGAACGATGA+TGG + chr5_1:47415099-47415118 None:intergenic 30.0%
GTGAAGTGAAGTAATGAATA+TGG + chr5_1:47415110-47415129 None:intergenic 30.0%
TGTGTAATAGTGAGTGTTAA+TGG + chr5_1:47415197-47415216 None:intergenic 30.0%
TTCCAGAAGTAGTAGTTATT+CGG + chr5_1:47415351-47415370 None:intergenic 30.0%
! TACTACTTCTGGAAACAAAT+GGG - chr5_1:47415357-47415376 Msa0733890:CDS 30.0%
TGGAGGAGTTTATTAAAAAG+GGG - chr5_1:47415544-47415563 Msa0733890:intron 30.0%
TGATTATAAGAAGGAGCTTA+AGG - chr5_1:47415626-47415645 Msa0733890:intron 30.0%
TTGAATCGAATCCTTAAAGT+TGG - chr5_1:47415752-47415771 Msa0733890:intron 30.0%
TGAATCGAATCCTTAAAGTT+GGG - chr5_1:47415753-47415772 Msa0733890:intron 30.0%
!!! TTTTTTTTCCTTACCGGATT+TGG - chr5_1:47415920-47415939 Msa0733890:five_prime_UTR 30.0%
! TAAGTAATGGGATTGTTTAG+AGG - chr5_1:47415971-47415990 Msa0733890:five_prime_UTR 30.0%
! AAGTAATGGGATTGTTTAGA+GGG - chr5_1:47415972-47415991 Msa0733890:five_prime_UTR 30.0%
TTGGGAATGAGTGAAAAAAA+AGG + chr5_1:47414922-47414941 None:intergenic 30.0%
TAATGAATATGGAACGATGA+TGG + chr5_1:47415099-47415118 None:intergenic 30.0%
GTGAAGTGAAGTAATGAATA+TGG + chr5_1:47415110-47415129 None:intergenic 30.0%
TGTGTAATAGTGAGTGTTAA+TGG + chr5_1:47415197-47415216 None:intergenic 30.0%
TTCCAGAAGTAGTAGTTATT+CGG + chr5_1:47415351-47415370 None:intergenic 30.0%
! TACTACTTCTGGAAACAAAT+GGG - chr5_1:47415357-47415376 Msa0733890:CDS 30.0%
TGGAGGAGTTTATTAAAAAG+GGG - chr5_1:47415544-47415563 Msa0733890:intron 30.0%
TGATTATAAGAAGGAGCTTA+AGG - chr5_1:47415626-47415645 Msa0733890:intron 30.0%
TTGAATCGAATCCTTAAAGT+TGG - chr5_1:47415752-47415771 Msa0733890:intron 30.0%
TGAATCGAATCCTTAAAGTT+GGG - chr5_1:47415753-47415772 Msa0733890:intron 30.0%
!!! TTTTTTTTCCTTACCGGATT+TGG - chr5_1:47415920-47415939 Msa0733890:five_prime_UTR 30.0%
! TAAGTAATGGGATTGTTTAG+AGG - chr5_1:47415971-47415990 Msa0733890:five_prime_UTR 30.0%
! AAGTAATGGGATTGTTTAGA+GGG - chr5_1:47415972-47415991 Msa0733890:five_prime_UTR 30.0%
ATAATATGAAGATTGCCACG+AGG + chr5_1:47414842-47414861 None:intergenic 35.0%
AAAAAGGCGAGAAAGAAAGT+TGG + chr5_1:47414906-47414925 None:intergenic 35.0%
ACAATCTAACATTGCACACA+AGG - chr5_1:47415214-47415233 Msa0733890:CDS 35.0%
! CTACTACTTCTGGAAACAAA+TGG - chr5_1:47415356-47415375 Msa0733890:CDS 35.0%
! ACTACTTCTGGAAACAAATG+GGG - chr5_1:47415358-47415377 Msa0733890:CDS 35.0%
GGAGGAGTTTATTAAAAAGG+GGG - chr5_1:47415545-47415564 Msa0733890:intron 35.0%
GAGGAGTTTATTAAAAAGGG+GGG - chr5_1:47415546-47415565 Msa0733890:intron 35.0%
AGGAGTTTATTAAAAAGGGG+GGG - chr5_1:47415547-47415566 Msa0733890:intron 35.0%
GAACAACTATTGGACCTAAA+GGG - chr5_1:47415577-47415596 Msa0733890:intron 35.0%
GGATGAGAGTGATTATAAGA+AGG - chr5_1:47415617-47415636 Msa0733890:intron 35.0%
AGGATGAGGAATGAAGTTAT+TGG - chr5_1:47415687-47415706 Msa0733890:exon 35.0%
GAATCGAATCCTTAAAGTTG+GGG - chr5_1:47415754-47415773 Msa0733890:intron 35.0%
ATAATATGAAGATTGCCACG+AGG + chr5_1:47414842-47414861 None:intergenic 35.0%
AAAAAGGCGAGAAAGAAAGT+TGG + chr5_1:47414906-47414925 None:intergenic 35.0%
ACAATCTAACATTGCACACA+AGG - chr5_1:47415214-47415233 Msa0733890:CDS 35.0%
! CTACTACTTCTGGAAACAAA+TGG - chr5_1:47415356-47415375 Msa0733890:CDS 35.0%
! ACTACTTCTGGAAACAAATG+GGG - chr5_1:47415358-47415377 Msa0733890:CDS 35.0%
GGAGGAGTTTATTAAAAAGG+GGG - chr5_1:47415545-47415564 Msa0733890:intron 35.0%
GAGGAGTTTATTAAAAAGGG+GGG - chr5_1:47415546-47415565 Msa0733890:intron 35.0%
AGGAGTTTATTAAAAAGGGG+GGG - chr5_1:47415547-47415566 Msa0733890:intron 35.0%
GAACAACTATTGGACCTAAA+GGG - chr5_1:47415577-47415596 Msa0733890:intron 35.0%
GGATGAGAGTGATTATAAGA+AGG - chr5_1:47415617-47415636 Msa0733890:intron 35.0%
AGGATGAGGAATGAAGTTAT+TGG - chr5_1:47415687-47415706 Msa0733890:exon 35.0%
GAATCGAATCCTTAAAGTTG+GGG - chr5_1:47415754-47415773 Msa0733890:intron 35.0%
AGTGCTTCTGCAAAGGAAAA+TGG + chr5_1:47414871-47414890 None:intergenic 40.0%
!! GATGATGGTGTTTTACTGCA+GGG + chr5_1:47415084-47415103 None:intergenic 40.0%
!!! AATGTACCTGTTTTGGGTTG+CGG + chr5_1:47415167-47415186 None:intergenic 40.0%
GACCGAATAACTACTACTTC+TGG - chr5_1:47415346-47415365 Msa0733890:CDS 40.0%
AGCTGGAACATTTGTATCAG+CGG - chr5_1:47415487-47415506 Msa0733890:CDS 40.0%
ATTAAAAAGGGGGGGATGAT+TGG - chr5_1:47415555-47415574 Msa0733890:intron 40.0%
GGGATGATTGGAACAACTAT+TGG - chr5_1:47415567-47415586 Msa0733890:intron 40.0%
GGAACAACTATTGGACCTAA+AGG - chr5_1:47415576-47415595 Msa0733890:intron 40.0%
AACTATTGGACCTAAAGGGA+TGG - chr5_1:47415581-47415600 Msa0733890:intron 40.0%
GGATAAGAAGTTTGAGCAAG+AGG - chr5_1:47415647-47415666 Msa0733890:intron 40.0%
! AGAAGCTTTGGTTTAGGATG+AGG - chr5_1:47415673-47415692 Msa0733890:CDS 40.0%
TGAAGTTATTGGTGAGCTTC+AGG - chr5_1:47415698-47415717 Msa0733890:five_prime_UTR 40.0%
AAGCACAAAGAAGCCAAATC+CGG + chr5_1:47415936-47415955 None:intergenic 40.0%
AGTGCTTCTGCAAAGGAAAA+TGG + chr5_1:47414871-47414890 None:intergenic 40.0%
!! GATGATGGTGTTTTACTGCA+GGG + chr5_1:47415084-47415103 None:intergenic 40.0%
!!! AATGTACCTGTTTTGGGTTG+CGG + chr5_1:47415167-47415186 None:intergenic 40.0%
GACCGAATAACTACTACTTC+TGG - chr5_1:47415346-47415365 Msa0733890:CDS 40.0%
AGCTGGAACATTTGTATCAG+CGG - chr5_1:47415487-47415506 Msa0733890:CDS 40.0%
ATTAAAAAGGGGGGGATGAT+TGG - chr5_1:47415555-47415574 Msa0733890:intron 40.0%
GGGATGATTGGAACAACTAT+TGG - chr5_1:47415567-47415586 Msa0733890:intron 40.0%
GGAACAACTATTGGACCTAA+AGG - chr5_1:47415576-47415595 Msa0733890:intron 40.0%
AACTATTGGACCTAAAGGGA+TGG - chr5_1:47415581-47415600 Msa0733890:intron 40.0%
GGATAAGAAGTTTGAGCAAG+AGG - chr5_1:47415647-47415666 Msa0733890:intron 40.0%
! AGAAGCTTTGGTTTAGGATG+AGG - chr5_1:47415673-47415692 Msa0733890:CDS 40.0%
TGAAGTTATTGGTGAGCTTC+AGG - chr5_1:47415698-47415717 Msa0733890:five_prime_UTR 40.0%
AAGCACAAAGAAGCCAAATC+CGG + chr5_1:47415936-47415955 None:intergenic 40.0%
TTTCACTCATTCCCAATCCC+AGG - chr5_1:47414926-47414945 Msa0733890:three_prime_UTR 45.0%
! TGTGTTTGTTAAGCGCACCT+GGG + chr5_1:47414946-47414965 None:intergenic 45.0%
! ATGTGTTTGTTAAGCGCACC+TGG + chr5_1:47414947-47414966 None:intergenic 45.0%
!! CGATGATGGTGTTTTACTGC+AGG + chr5_1:47415085-47415104 None:intergenic 45.0%
!! GTTTCGAAGAAGCGTTTACG+AGG + chr5_1:47415143-47415162 None:intergenic 45.0%
! CTTCTGGAAACAAATGGGGA+AGG - chr5_1:47415362-47415381 Msa0733890:CDS 45.0%
GAAACAAATGGGGAAGGCTT+TGG - chr5_1:47415368-47415387 Msa0733890:CDS 45.0%
TCTTTCTCTTCCTTCGGCAT+TGG + chr5_1:47415468-47415487 None:intergenic 45.0%
TCCAGCTCTTTCTCTTCCTT+CGG + chr5_1:47415474-47415493 None:intergenic 45.0%
TATTGGACCTAAAGGGATGG+TGG - chr5_1:47415584-47415603 Msa0733890:intron 45.0%
TCATTCTCCACCATCCCTTT+AGG + chr5_1:47415594-47415613 None:intergenic 45.0%
!! AGGGATGGTGGAGAATGATA+TGG - chr5_1:47415596-47415615 Msa0733890:intron 45.0%
! AGGCTCAGAAGCTTTGGTTT+AGG - chr5_1:47415667-47415686 Msa0733890:CDS 45.0%
TATTGGTGAGCTTCAGGAGA+AGG - chr5_1:47415704-47415723 Msa0733890:five_prime_UTR 45.0%
ATTGGTGAGCTTCAGGAGAA+GGG - chr5_1:47415705-47415724 Msa0733890:five_prime_UTR 45.0%
CAAAGAAGCCAAATCCGGTA+AGG + chr5_1:47415931-47415950 None:intergenic 45.0%
TTTCACTCATTCCCAATCCC+AGG - chr5_1:47414926-47414945 Msa0733890:three_prime_UTR 45.0%
! TGTGTTTGTTAAGCGCACCT+GGG + chr5_1:47414946-47414965 None:intergenic 45.0%
! ATGTGTTTGTTAAGCGCACC+TGG + chr5_1:47414947-47414966 None:intergenic 45.0%
!! CGATGATGGTGTTTTACTGC+AGG + chr5_1:47415085-47415104 None:intergenic 45.0%
!! GTTTCGAAGAAGCGTTTACG+AGG + chr5_1:47415143-47415162 None:intergenic 45.0%
! CTTCTGGAAACAAATGGGGA+AGG - chr5_1:47415362-47415381 Msa0733890:CDS 45.0%
GAAACAAATGGGGAAGGCTT+TGG - chr5_1:47415368-47415387 Msa0733890:CDS 45.0%
TCTTTCTCTTCCTTCGGCAT+TGG + chr5_1:47415468-47415487 None:intergenic 45.0%
TCCAGCTCTTTCTCTTCCTT+CGG + chr5_1:47415474-47415493 None:intergenic 45.0%
TATTGGACCTAAAGGGATGG+TGG - chr5_1:47415584-47415603 Msa0733890:intron 45.0%
TCATTCTCCACCATCCCTTT+AGG + chr5_1:47415594-47415613 None:intergenic 45.0%
!! AGGGATGGTGGAGAATGATA+TGG - chr5_1:47415596-47415615 Msa0733890:intron 45.0%
! AGGCTCAGAAGCTTTGGTTT+AGG - chr5_1:47415667-47415686 Msa0733890:CDS 45.0%
TATTGGTGAGCTTCAGGAGA+AGG - chr5_1:47415704-47415723 Msa0733890:five_prime_UTR 45.0%
ATTGGTGAGCTTCAGGAGAA+GGG - chr5_1:47415705-47415724 Msa0733890:five_prime_UTR 45.0%
CAAAGAAGCCAAATCCGGTA+AGG + chr5_1:47415931-47415950 None:intergenic 45.0%
!! ATAAAATGTAAAAAATTATT+GGG + chr5_1:47415259-47415278 None:intergenic 5.0%
!! ATAAAATGTAAAAAATTATT+GGG + chr5_1:47415259-47415278 None:intergenic 5.0%
CTCGACAAGTGCTTCTGCAA+AGG + chr5_1:47414878-47414897 None:intergenic 50.0%
TGTTAAGCGCACCTGGGATT+GGG + chr5_1:47414940-47414959 None:intergenic 50.0%
TTGTTAAGCGCACCTGGGAT+TGG + chr5_1:47414941-47414960 None:intergenic 50.0%
GAAACACCGCAACCCAAAAC+AGG - chr5_1:47415158-47415177 Msa0733890:CDS 50.0%
GCGTGCACTTATGCAGTGAT+GGG - chr5_1:47415390-47415409 Msa0733890:CDS 50.0%
!! CACTTATGCAGTGATGGGTG+CGG - chr5_1:47415395-47415414 Msa0733890:CDS 50.0%
ATTCAACGAGACGTCGCCAT+TGG - chr5_1:47415425-47415444 Msa0733890:CDS 50.0%
TTCAACGAGACGTCGCCATT+GGG - chr5_1:47415426-47415445 Msa0733890:CDS 50.0%
TTCAACGTCCACCAACCCAA+TGG + chr5_1:47415444-47415463 None:intergenic 50.0%
GTTTCCATATCCAGGAGACG+AGG - chr5_1:47415515-47415534 Msa0733890:intron 50.0%
AGCAAGAGGCTCAGAAGCTT+TGG - chr5_1:47415661-47415680 Msa0733890:CDS 50.0%
CTCGACAAGTGCTTCTGCAA+AGG + chr5_1:47414878-47414897 None:intergenic 50.0%
TGTTAAGCGCACCTGGGATT+GGG + chr5_1:47414940-47414959 None:intergenic 50.0%
TTGTTAAGCGCACCTGGGAT+TGG + chr5_1:47414941-47414960 None:intergenic 50.0%
GAAACACCGCAACCCAAAAC+AGG - chr5_1:47415158-47415177 Msa0733890:CDS 50.0%
GCGTGCACTTATGCAGTGAT+GGG - chr5_1:47415390-47415409 Msa0733890:CDS 50.0%
!! CACTTATGCAGTGATGGGTG+CGG - chr5_1:47415395-47415414 Msa0733890:CDS 50.0%
ATTCAACGAGACGTCGCCAT+TGG - chr5_1:47415425-47415444 Msa0733890:CDS 50.0%
TTCAACGAGACGTCGCCATT+GGG - chr5_1:47415426-47415445 Msa0733890:CDS 50.0%
TTCAACGTCCACCAACCCAA+TGG + chr5_1:47415444-47415463 None:intergenic 50.0%
GTTTCCATATCCAGGAGACG+AGG - chr5_1:47415515-47415534 Msa0733890:intron 50.0%
AGCAAGAGGCTCAGAAGCTT+TGG - chr5_1:47415661-47415680 Msa0733890:CDS 50.0%
GGCGTGCACTTATGCAGTGA+TGG - chr5_1:47415389-47415408 Msa0733890:CDS 55.0%
GACGTTGAAGCCAATGCCGA+AGG - chr5_1:47415455-47415474 Msa0733890:CDS 55.0%
GCCGAAGGAAGAGAAAGAGC+TGG - chr5_1:47415470-47415489 Msa0733890:CDS 55.0%
CGGCGTGAGTTTCCATATCC+AGG - chr5_1:47415507-47415526 Msa0733890:intron 55.0%
GCTTCCTCGTCTCCTGGATA+TGG + chr5_1:47415522-47415541 None:intergenic 55.0%
TCCAGGAGACGAGGAAGCTA+TGG - chr5_1:47415524-47415543 Msa0733890:intron 55.0%
TCCATAGCTTCCTCGTCTCC+TGG + chr5_1:47415528-47415547 None:intergenic 55.0%
AGGAGACGAGGAAGCTATGG+AGG - chr5_1:47415527-47415546 Msa0733890:intron 55.0%
GGCGTGCACTTATGCAGTGA+TGG - chr5_1:47415389-47415408 Msa0733890:CDS 55.0%
GACGTTGAAGCCAATGCCGA+AGG - chr5_1:47415455-47415474 Msa0733890:CDS 55.0%
GCCGAAGGAAGAGAAAGAGC+TGG - chr5_1:47415470-47415489 Msa0733890:CDS 55.0%
CGGCGTGAGTTTCCATATCC+AGG - chr5_1:47415507-47415526 Msa0733890:intron 55.0%
GCTTCCTCGTCTCCTGGATA+TGG + chr5_1:47415522-47415541 None:intergenic 55.0%
TCCAGGAGACGAGGAAGCTA+TGG - chr5_1:47415524-47415543 Msa0733890:intron 55.0%
TCCATAGCTTCCTCGTCTCC+TGG + chr5_1:47415528-47415547 None:intergenic 55.0%
AGGAGACGAGGAAGCTATGG+AGG - chr5_1:47415527-47415546 Msa0733890:intron 55.0%
ACGAGACGTCGCCATTGGGT+TGG - chr5_1:47415430-47415449 Msa0733890:CDS 60.0%
!! AGACGTCGCCATTGGGTTGG+TGG - chr5_1:47415433-47415452 Msa0733890:CDS 60.0%
ACGAGACGTCGCCATTGGGT+TGG - chr5_1:47415430-47415449 Msa0733890:CDS 60.0%
!! AGACGTCGCCATTGGGTTGG+TGG - chr5_1:47415433-47415452 Msa0733890:CDS 60.0%
!! ATGCAGTGATGGGTGCGGCG+TGG - chr5_1:47415400-47415419 Msa0733890:CDS 65.0%
!! ATGCAGTGATGGGTGCGGCG+TGG - chr5_1:47415400-47415419 Msa0733890:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 47414825 47416004 47414825 ID=Msa0733890;Name=Msa0733890
chr5_1 mRNA 47414825 47416004 47414825 ID=Msa0733890-mRNA-1;Parent=Msa0733890;Name=Msa0733890-mRNA-1;_AED=0.00;_eAED=0.00;_QI=171|1|1|1|0.5|0.33|3|258|161
chr5_1 exon 47414825 47415525 47414825 ID=Msa0733890-mRNA-1:exon:13577;Parent=Msa0733890-mRNA-1
chr5_1 exon 47415650 47415740 47415650 ID=Msa0733890-mRNA-1:exon:13576;Parent=Msa0733890-mRNA-1
chr5_1 exon 47415882 47416004 47415882 ID=Msa0733890-mRNA-1:exon:13575;Parent=Msa0733890-mRNA-1
chr5_1 five_prime_UTR 47415882 47416004 47415882 ID=Msa0733890-mRNA-1:five_prime_utr;Parent=Msa0733890-mRNA-1
chr5_1 five_prime_UTR 47415693 47415740 47415693 ID=Msa0733890-mRNA-1:five_prime_utr;Parent=Msa0733890-mRNA-1
chr5_1 CDS 47415650 47415692 47415650 ID=Msa0733890-mRNA-1:cds;Parent=Msa0733890-mRNA-1
chr5_1 CDS 47415083 47415525 47415083 ID=Msa0733890-mRNA-1:cds;Parent=Msa0733890-mRNA-1
chr5_1 three_prime_UTR 47414825 47415082 47414825 ID=Msa0733890-mRNA-1:three_prime_utr;Parent=Msa0733890-mRNA-1
Gene Sequence

>Msa0733890

ATGCCTCGTAAACGCTTCTTCGAAACACCGCAACCCAAAACAGGTTTAGAACATGCCCAATATTTTCTAAAGAAAATAGGGTTGGGACCGAATAACTACTACTTCTGGAAACAAATGGGGAAGGCTTTGGCGTGCACTTATGCAGTGATGGGTGCGGCGTGGCTATTCAACGAGACGTCGCCATTGGGTTGGTGGACGTTGAAGCCAATGCCGAAGGAAGAGAAAGAGCTGGAACATTTGTATCAGCGGCGTGAGTTTCCATATCCAGGAGACGAGGAAGCTATGGAGGAGTTTATTAAAAAGGGGGGGATGATTGGAACAACTATTGGACCTAAAGGGATGGTGGAGAATGATATGGATGAGAGTGATTATAAGAAGGAGCTTAAGGATAAGAAGTTTGAGCAAGAGGCTCAGAAGCTTTGGTTTAGGATGAGGAATGAAGTTATTGGTGAGCTTCAGGAGAAGGGTTTTGATGTAGATCAATGA
Protein sequence

>Msa0733890

MPRKRFFETPQPKTGLEHAQYFLKKIGLGPNNYYFWKQMGKALACTYAVMGAAWLFNETSPLGWWTLKPMPKEEKELEHLYQRREFPYPGDEEAMEEFIKKGGMIGTTIGPKGMVENDMDESDYKKELKDKKFEQEAQKLWFRMRNEVIGELQEKGFDVDQ*