AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1461490


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1461490 MtrunA17_Chr8g0344731 99.038 104 1 0 1 104 1 104 7.92e-72 213
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1461490 AT1G17350.3 63.810 105 37 1 1 104 1 105 3.21e-45 146
Msa1461490 AT1G17350.5 63.810 105 37 1 1 104 1 105 3.21e-45 146
Msa1461490 AT1G17350.1 63.810 105 37 1 1 104 1 105 3.21e-45 146
Msa1461490 AT1G17350.4 63.810 105 37 1 1 104 1 105 3.21e-45 146
Msa1461490 AT1G17350.2 63.810 105 37 1 1 104 1 105 4.95e-45 146
Msa1461490 AT1G72420.3 60.952 105 40 1 1 104 1 105 6.95e-42 135
Msa1461490 AT1G72420.6 60.952 105 40 1 1 104 1 105 6.95e-42 135
Msa1461490 AT1G72420.5 60.952 105 40 1 1 104 1 105 1.53e-41 135
Msa1461490 AT1G72420.1 60.952 105 40 1 1 104 1 105 1.04e-40 134
Msa1461490 AT1G72420.2 60.952 105 40 1 1 104 1 105 1.04e-40 134
Msa1461490 AT1G72420.4 59.091 88 35 1 18 104 7 94 1.10e-31 111

Find 21 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTATTCTGATTCTGAATT+TGG 0.164131 tig0041568:-1314 Msa1461490:CDS
GAAAACAACTTATAGCTTAT+AGG 0.244490 tig0041568:+823 None:intergenic
ATTACTAGTGTTGTCAAATA+TGG 0.280574 tig0041568:-961 Msa1461490:three_prime_UTR
CAGATACCTGAATCTGAAAA+TGG 0.295053 tig0041568:-1170 Msa1461490:CDS
TCAAAGACTTTAGTGTTGTT+TGG 0.324228 tig0041568:-1030 Msa1461490:exon
CAATGTTAATATATTATTGA+TGG 0.329432 tig0041568:+912 None:intergenic
TTGATTTCAACAGCTATATC+TGG 0.331646 tig0041568:-1416 Msa1461490:intron
AAGCTATCTTGTAGAGCTTA+TGG 0.342086 tig0041568:-798 Msa1461490:three_prime_UTR
TTGTGGAATGCGCTCCAAAA+AGG 0.350095 tig0041568:-1066 Msa1461490:CDS
CCAAGCAAGAATTATCAAAA+TGG 0.360136 tig0041568:-1340 Msa1461490:CDS
TCTGAAAATGGTAAAACTAC+TGG 0.430983 tig0041568:-1158 Msa1461490:CDS
CTCTGGGAATCTTTCACTTG+AGG 0.435126 tig0041568:-1129 Msa1461490:CDS
TATTCTGATTCTGAATTTGG+AGG 0.441704 tig0041568:-1311 Msa1461490:intron
TCAATGGATGCTACTAAGAA+AGG 0.500994 tig0041568:-1498 Msa1461490:intron
TAGTAGCATCCATTGATGCT+TGG 0.514719 tig0041568:+1505 None:intergenic
CTTTCACTTGAGGTTACTCA+AGG 0.540859 tig0041568:-1119 Msa1461490:CDS
AAGACTGTTCCAAGCATCAA+TGG 0.543179 tig0041568:-1514 Msa1461490:CDS
AAGTGGAATATTAGTAGAGG+TGG 0.553563 tig0041568:-1092 Msa1461490:CDS
GCTAAGTGGAATATTAGTAG+AGG 0.567692 tig0041568:-1095 Msa1461490:CDS
AGGTTACTCAAGGTGCTAAG+TGG 0.593770 tig0041568:-1109 Msa1461490:CDS
TGTCAAATATGGCGCCCTGA+CGG 0.601840 tig0041568:-950 Msa1461490:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTTGATATAGAAAT+AGG + tig0041568:1474-1493 None:intergenic 10.0%
!!! TTATTTTTTGATATAGAAAT+AGG + tig0041568:1474-1493 None:intergenic 10.0%
!!! AAGTTGAATATATATTTTTC+AGG + tig0041568:971-990 None:intergenic 15.0%
!! CAATGTTAATATATTATTGA+TGG + tig0041568:1427-1446 None:intergenic 15.0%
!!! AAGTTGAATATATATTTTTC+AGG + tig0041568:971-990 None:intergenic 15.0%
!! CAATGTTAATATATTATTGA+TGG + tig0041568:1427-1446 None:intergenic 15.0%
!!! TTGAATATATATTTTTCAGG+TGG + tig0041568:968-987 None:intergenic 20.0%
!!! ACTTTGGTTAATTTAATTTC+TGG - tig0041568:1078-1097 Msa1461490:CDS 20.0%
!!! CTTTGGTTAATTTAATTTCT+GGG - tig0041568:1079-1098 Msa1461490:CDS 20.0%
!!! TTGAATATATATTTTTCAGG+TGG + tig0041568:968-987 None:intergenic 20.0%
!!! ACTTTGGTTAATTTAATTTC+TGG - tig0041568:1078-1097 Msa1461490:CDS 20.0%
!!! CTTTGGTTAATTTAATTTCT+GGG - tig0041568:1079-1098 Msa1461490:CDS 20.0%
!!! AATATATATTTTTCAGGTGG+TGG + tig0041568:965-984 None:intergenic 25.0%
! TTGTATTCTGATTCTGAATT+TGG - tig0041568:1022-1041 Msa1461490:exon 25.0%
! TCAAATTGTTAATGTTGTTC+AGG - tig0041568:1124-1143 Msa1461490:CDS 25.0%
! ATTACTAGTGTTGTCAAATA+TGG - tig0041568:1375-1394 Msa1461490:CDS 25.0%
! GAAAACAACTTATAGCTTAT+AGG + tig0041568:1516-1535 None:intergenic 25.0%
!!! AATATATATTTTTCAGGTGG+TGG + tig0041568:965-984 None:intergenic 25.0%
! TTGTATTCTGATTCTGAATT+TGG - tig0041568:1022-1041 Msa1461490:exon 25.0%
! TCAAATTGTTAATGTTGTTC+AGG - tig0041568:1124-1143 Msa1461490:CDS 25.0%
! ATTACTAGTGTTGTCAAATA+TGG - tig0041568:1375-1394 Msa1461490:CDS 25.0%
! GAAAACAACTTATAGCTTAT+AGG + tig0041568:1516-1535 None:intergenic 25.0%
TTGATTTCAACAGCTATATC+TGG - tig0041568:920-939 Msa1461490:three_prime_UTR 30.0%
!! CCATTTTGATAATTCTTGCT+TGG + tig0041568:999-1018 None:intergenic 30.0%
CCAAGCAAGAATTATCAAAA+TGG - tig0041568:996-1015 Msa1461490:three_prime_UTR 30.0%
TATTCTGATTCTGAATTTGG+AGG - tig0041568:1025-1044 Msa1461490:exon 30.0%
! GTTGCTAAAGTTTGAAACTT+TGG - tig0041568:1062-1081 Msa1461490:CDS 30.0%
! GTTTTACCATTTTCAGATTC+AGG + tig0041568:1175-1194 None:intergenic 30.0%
TCTGAAAATGGTAAAACTAC+TGG - tig0041568:1178-1197 Msa1461490:CDS 30.0%
! AAAACTACTGGAATTTTCTC+TGG - tig0041568:1190-1209 Msa1461490:CDS 30.0%
! AAACTACTGGAATTTTCTCT+GGG - tig0041568:1191-1210 Msa1461490:CDS 30.0%
! AGTTAAAAAAGCCACCTTTT+TGG + tig0041568:1287-1306 None:intergenic 30.0%
! TCAAAGACTTTAGTGTTGTT+TGG - tig0041568:1306-1325 Msa1461490:intron 30.0%
TTGATTTCAACAGCTATATC+TGG - tig0041568:920-939 Msa1461490:three_prime_UTR 30.0%
!! CCATTTTGATAATTCTTGCT+TGG + tig0041568:999-1018 None:intergenic 30.0%
CCAAGCAAGAATTATCAAAA+TGG - tig0041568:996-1015 Msa1461490:three_prime_UTR 30.0%
TATTCTGATTCTGAATTTGG+AGG - tig0041568:1025-1044 Msa1461490:exon 30.0%
! GTTGCTAAAGTTTGAAACTT+TGG - tig0041568:1062-1081 Msa1461490:CDS 30.0%
! GTTTTACCATTTTCAGATTC+AGG + tig0041568:1175-1194 None:intergenic 30.0%
TCTGAAAATGGTAAAACTAC+TGG - tig0041568:1178-1197 Msa1461490:CDS 30.0%
! AAAACTACTGGAATTTTCTC+TGG - tig0041568:1190-1209 Msa1461490:CDS 30.0%
! AAACTACTGGAATTTTCTCT+GGG - tig0041568:1191-1210 Msa1461490:CDS 30.0%
! AGTTAAAAAAGCCACCTTTT+TGG + tig0041568:1287-1306 None:intergenic 30.0%
! TCAAAGACTTTAGTGTTGTT+TGG - tig0041568:1306-1325 Msa1461490:intron 30.0%
TCAATGGATGCTACTAAGAA+AGG - tig0041568:838-857 Msa1461490:three_prime_UTR 35.0%
CAGATACCTGAATCTGAAAA+TGG - tig0041568:1166-1185 Msa1461490:CDS 35.0%
GCTAAGTGGAATATTAGTAG+AGG - tig0041568:1241-1260 Msa1461490:intron 35.0%
AAGTGGAATATTAGTAGAGG+TGG - tig0041568:1244-1263 Msa1461490:intron 35.0%
!! ATTAGTAGAGGTGGATTTTG+TGG - tig0041568:1253-1272 Msa1461490:intron 35.0%
AAGCTATCTTGTAGAGCTTA+TGG - tig0041568:1538-1557 Msa1461490:exon 35.0%
TCAATGGATGCTACTAAGAA+AGG - tig0041568:838-857 Msa1461490:three_prime_UTR 35.0%
CAGATACCTGAATCTGAAAA+TGG - tig0041568:1166-1185 Msa1461490:CDS 35.0%
GCTAAGTGGAATATTAGTAG+AGG - tig0041568:1241-1260 Msa1461490:intron 35.0%
AAGTGGAATATTAGTAGAGG+TGG - tig0041568:1244-1263 Msa1461490:intron 35.0%
!! ATTAGTAGAGGTGGATTTTG+TGG - tig0041568:1253-1272 Msa1461490:intron 35.0%
AAGCTATCTTGTAGAGCTTA+TGG - tig0041568:1538-1557 Msa1461490:exon 35.0%
AAGACTGTTCCAAGCATCAA+TGG - tig0041568:822-841 Msa1461490:three_prime_UTR 40.0%
TAGTAGCATCCATTGATGCT+TGG + tig0041568:834-853 None:intergenic 40.0%
CTTTCACTTGAGGTTACTCA+AGG - tig0041568:1217-1236 Msa1461490:intron 40.0%
AGAAAGCCAAAAATCCGTCA+GGG + tig0041568:1403-1422 None:intergenic 40.0%
AAGACTGTTCCAAGCATCAA+TGG - tig0041568:822-841 Msa1461490:three_prime_UTR 40.0%
TAGTAGCATCCATTGATGCT+TGG + tig0041568:834-853 None:intergenic 40.0%
CTTTCACTTGAGGTTACTCA+AGG - tig0041568:1217-1236 Msa1461490:intron 40.0%
AGAAAGCCAAAAATCCGTCA+GGG + tig0041568:1403-1422 None:intergenic 40.0%
! CTCTGGGAATCTTTCACTTG+AGG - tig0041568:1207-1226 Msa1461490:intron 45.0%
! AGGTTACTCAAGGTGCTAAG+TGG - tig0041568:1227-1246 Msa1461490:intron 45.0%
TTGTGGAATGCGCTCCAAAA+AGG - tig0041568:1270-1289 Msa1461490:intron 45.0%
CAGAAAGCCAAAAATCCGTC+AGG + tig0041568:1404-1423 None:intergenic 45.0%
! CTCTGGGAATCTTTCACTTG+AGG - tig0041568:1207-1226 Msa1461490:intron 45.0%
! AGGTTACTCAAGGTGCTAAG+TGG - tig0041568:1227-1246 Msa1461490:intron 45.0%
TTGTGGAATGCGCTCCAAAA+AGG - tig0041568:1270-1289 Msa1461490:intron 45.0%
CAGAAAGCCAAAAATCCGTC+AGG + tig0041568:1404-1423 None:intergenic 45.0%
TGGAATGCGCTCCAAAAAGG+TGG - tig0041568:1273-1292 Msa1461490:intron 50.0%
TGTCAAATATGGCGCCCTGA+CGG - tig0041568:1386-1405 Msa1461490:CDS 50.0%
TGGAATGCGCTCCAAAAAGG+TGG - tig0041568:1273-1292 Msa1461490:intron 50.0%
TGTCAAATATGGCGCCCTGA+CGG - tig0041568:1386-1405 Msa1461490:CDS 50.0%
!!! ATGGCGCCCTGACGGATTTT+TGG - tig0041568:1394-1413 Msa1461490:CDS 55.0%
!!! ATGGCGCCCTGACGGATTTT+TGG - tig0041568:1394-1413 Msa1461490:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0041568 gene 785 1573 785 ID=Msa1461490;Name=Msa1461490
tig0041568 mRNA 785 1573 785 ID=Msa1461490-mRNA-1;Parent=Msa1461490;Name=Msa1461490-mRNA-1;_AED=0.03;_eAED=0.03;_QI=23|1|1|1|1|1|3|255|112
tig0041568 exon 785 1212 785 ID=Msa1461490-mRNA-1:exon:23215;Parent=Msa1461490-mRNA-1
tig0041568 exon 1312 1425 1312 ID=Msa1461490-mRNA-1:exon:23214;Parent=Msa1461490-mRNA-1
tig0041568 exon 1499 1573 1499 ID=Msa1461490-mRNA-1:exon:23213;Parent=Msa1461490-mRNA-1
tig0041568 five_prime_UTR 1551 1573 1551 ID=Msa1461490-mRNA-1:five_prime_utr;Parent=Msa1461490-mRNA-1
tig0041568 CDS 1499 1550 1499 ID=Msa1461490-mRNA-1:cds;Parent=Msa1461490-mRNA-1
tig0041568 CDS 1312 1425 1312 ID=Msa1461490-mRNA-1:cds;Parent=Msa1461490-mRNA-1
tig0041568 CDS 1040 1212 1040 ID=Msa1461490-mRNA-1:cds;Parent=Msa1461490-mRNA-1
tig0041568 three_prime_UTR 785 1039 785 ID=Msa1461490-mRNA-1:three_prime_utr;Parent=Msa1461490-mRNA-1
Gene Sequence

>Msa1461490

ATGTCAAGAATACGAAGACTGTTCCAAGCATCAATGGATGCTACTAAGAAAGCTATATCTGGTAGTTTTGATGATTTGATGCCACCACCTGAAAAATATATATTCAACTTCAATTCCAAGCAAGAATTATCAAAATGGCATTTGTATTCTGATTCTGAATTTGGAGGTTTGTCATCTGCATCATTGCAGATACCTGAATCTGAAAATGGTAAAACTACTGGAATTTTCTCTGGGAATCTTTCACTTGAGGTTACTCAAGGTGCTAAGTGGAATATTAGTAGAGGTGGATTTTGTGGAATGCGCTCCAAAAAGGTGGCTTTTTTAACTCAAAGACTTTAG

Protein sequence

>Msa1461490

MSRIRRLFQASMDATKKAISGSFDDLMPPPEKYIFNFNSKQELSKWHLYSDSEFGGLSSASLQIPESENGKTTGIFSGNLSLEVTQGAKWNISRGGFCGMRSKKVAFLTQRL*