AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0128020


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0128020 MtrunA17_Chr8g0345501 65.169 89 31 0 24 112 408 496 7.13e-34 123
Msa0128020 MtrunA17_Chr2g0313001 72.500 40 11 0 7 46 1 40 2.43e-13 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 33 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACTATCTCGAGCCAAATT+TGG 0.138676 1_3:-57670927 None:intergenic
TTACCTCTTTATCCCAAATT+TGG 0.184345 1_3:+57670914 Msa0128020:three_prime_UTR
CCATCACCATCTGGAATTAT+TGG 0.275118 1_3:-57669785 None:intergenic
AAGAAATTGCTCACATTATA+TGG 0.286562 1_3:+57670322 Msa0128020:CDS
TGATTTCTTTGACTCATTCA+TGG 0.333269 1_3:+57670401 Msa0128020:CDS
CCGTCATGGAAGCAATTATC+AGG 0.356730 1_3:+57670366 Msa0128020:CDS
AACTATCTCGAGCCAAATTT+GGG 0.363047 1_3:-57670926 None:intergenic
GTAGATTGAGTCAAAGTTGA+AGG 0.423072 1_3:+57670626 Msa0128020:CDS
ACTTGTCATGATGCATGTTA+TGG 0.428964 1_3:-57670724 None:intergenic
AATGTGTACATTTCAAGTTA+TGG 0.444448 1_3:+57669703 Msa0128020:exon
CAAACTCACCCATCACCATC+TGG 0.453372 1_3:-57669794 None:intergenic
CTCAGTTGATGCAAGAAGTT+CGG 0.458850 1_3:+57670599 Msa0128020:CDS
TACAAGGCTTAAGCAACAAT+TGG 0.471347 1_3:+57670656 Msa0128020:three_prime_UTR
TACATGGCAACCAGAGCATA+AGG 0.485592 1_3:+57670531 Msa0128020:CDS
CTAACATGGCTAGCTGAAGA+TGG 0.497267 1_3:-57669737 None:intergenic
TGCACATAGTAGTTCAGTTG+TGG 0.523431 1_3:+57669660 Msa0128020:five_prime_UTR
CCAATAATTCCAGATGGTGA+TGG 0.531511 1_3:+57669785 Msa0128020:CDS
GAGCCAAATTTGGGATAAAG+AGG 0.548288 1_3:-57670917 None:intergenic
CAATAATTCCAGATGGTGAT+GGG 0.552654 1_3:+57669786 Msa0128020:CDS
CAACGGTGTCTCTACTAACA+TGG 0.557176 1_3:-57669751 None:intergenic
CCTGATAATTGCTTCCATGA+CGG 0.572248 1_3:-57670366 None:intergenic
CATAGTAGTTCAGTTGTGGT+TGG 0.585520 1_3:+57669664 Msa0128020:five_prime_UTR
TAGAAGAGGGAAAACCGTCA+TGG 0.601838 1_3:+57670352 Msa0128020:CDS
TATATGGTCTCAATTAGAAG+AGG 0.602911 1_3:+57670338 Msa0128020:CDS
TTTCTTTAACTACCAATGTG+TGG 0.607987 1_3:-57670305 None:intergenic
AAAATACCAATAATTCCAGA+TGG 0.610541 1_3:+57669779 Msa0128020:CDS
AGTTGAAGGAGGCAACTACA+AGG 0.610652 1_3:+57670640 Msa0128020:exon
ATATGGTCTCAATTAGAAGA+GGG 0.627134 1_3:+57670339 Msa0128020:CDS
CTTGAAATGTACACATTGCG+AGG 0.634227 1_3:-57669697 None:intergenic
CAGGTTTGATCACCACACAT+TGG 0.634316 1_3:+57670293 Msa0128020:intron
GATTGAGTCAAAGTTGAAGG+AGG 0.637853 1_3:+57670629 Msa0128020:exon
ATGAGTCAAAGAAATCATCT+CGG 0.652827 1_3:-57670395 None:intergenic
CATGTTAGTAGAGACACCGT+TGG 0.707519 1_3:+57669752 Msa0128020:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CCAAATAATCATTATTATAT+GGG - chr1_3:57669825-57669844 None:intergenic 15.0%
!! TTATTTGGCTAATATAAATA+TGG + chr1_3:57669837-57669856 Msa0128020:intron 15.0%
!!! ATTTTGAATTAATATCTCTA+AGG + chr1_3:57670152-57670171 Msa0128020:intron 15.0%
!! CCAAATAATCATTATTATAT+GGG - chr1_3:57669825-57669844 None:intergenic 15.0%
!! TTATTTGGCTAATATAAATA+TGG + chr1_3:57669837-57669856 Msa0128020:intron 15.0%
!!! ATTTTGAATTAATATCTCTA+AGG + chr1_3:57670152-57670171 Msa0128020:intron 15.0%
!! CCCATATAATAATGATTATT+TGG + chr1_3:57669822-57669841 Msa0128020:intron 20.0%
!! GCCAAATAATCATTATTATA+TGG - chr1_3:57669826-57669845 None:intergenic 20.0%
!! TATTACAGAAAAAGTTTACA+TGG + chr1_3:57670515-57670534 Msa0128020:intron 20.0%
!! CCCATATAATAATGATTATT+TGG + chr1_3:57669822-57669841 Msa0128020:intron 20.0%
!! GCCAAATAATCATTATTATA+TGG - chr1_3:57669826-57669845 None:intergenic 20.0%
!! TATTACAGAAAAAGTTTACA+TGG + chr1_3:57670515-57670534 Msa0128020:intron 20.0%
!!! AATGTGTACATTTCAAGTTA+TGG + chr1_3:57669703-57669722 Msa0128020:exon 25.0%
!!! TATTGGTATTTTAGTACCAA+CGG - chr1_3:57669771-57669790 None:intergenic 25.0%
! AAAATACCAATAATTCCAGA+TGG + chr1_3:57669779-57669798 Msa0128020:CDS 25.0%
!! TTGTGGTATTATGATTTTAG+TGG + chr1_3:57669920-57669939 Msa0128020:intron 25.0%
!! TTTTCGTGTACATTATATTC+TGG + chr1_3:57669969-57669988 Msa0128020:intron 25.0%
!!! AATTAATATCTCTAAGGCTT+TGG + chr1_3:57670158-57670177 Msa0128020:intron 25.0%
!!! GTTTTTTCCTGAGAAAATAT+TGG + chr1_3:57670196-57670215 Msa0128020:intron 25.0%
!!! TTGTTTGAAACACATTTTTC+AGG + chr1_3:57670274-57670293 Msa0128020:intron 25.0%
! AAGAAATTGCTCACATTATA+TGG + chr1_3:57670322-57670341 Msa0128020:CDS 25.0%
!!! TTACTTAATCTGTTCTAAAG+AGG - chr1_3:57670465-57670484 None:intergenic 25.0%
!!! TGCGAAAAAACTTTGAAATA+AGG + chr1_3:57670560-57670579 Msa0128020:CDS 25.0%
!!! GCGAAAAAACTTTGAAATAA+GGG + chr1_3:57670561-57670580 Msa0128020:CDS 25.0%
!!! CGAAAAAACTTTGAAATAAG+GGG + chr1_3:57670562-57670581 Msa0128020:CDS 25.0%
! AGCATATGCACAAAAAAAAA+TGG - chr1_3:57670881-57670900 None:intergenic 25.0%
!!! TTTTGTGCATATGCTATTTT+TGG + chr1_3:57670886-57670905 Msa0128020:three_prime_UTR 25.0%
!!! AATGTGTACATTTCAAGTTA+TGG + chr1_3:57669703-57669722 Msa0128020:exon 25.0%
!!! TATTGGTATTTTAGTACCAA+CGG - chr1_3:57669771-57669790 None:intergenic 25.0%
! AAAATACCAATAATTCCAGA+TGG + chr1_3:57669779-57669798 Msa0128020:CDS 25.0%
!! TTGTGGTATTATGATTTTAG+TGG + chr1_3:57669920-57669939 Msa0128020:intron 25.0%
!! TTTTCGTGTACATTATATTC+TGG + chr1_3:57669969-57669988 Msa0128020:intron 25.0%
!!! AATTAATATCTCTAAGGCTT+TGG + chr1_3:57670158-57670177 Msa0128020:intron 25.0%
!!! GTTTTTTCCTGAGAAAATAT+TGG + chr1_3:57670196-57670215 Msa0128020:intron 25.0%
!!! TTGTTTGAAACACATTTTTC+AGG + chr1_3:57670274-57670293 Msa0128020:intron 25.0%
! AAGAAATTGCTCACATTATA+TGG + chr1_3:57670322-57670341 Msa0128020:CDS 25.0%
!!! TTACTTAATCTGTTCTAAAG+AGG - chr1_3:57670465-57670484 None:intergenic 25.0%
!!! TGCGAAAAAACTTTGAAATA+AGG + chr1_3:57670560-57670579 Msa0128020:CDS 25.0%
!!! GCGAAAAAACTTTGAAATAA+GGG + chr1_3:57670561-57670580 Msa0128020:CDS 25.0%
!!! CGAAAAAACTTTGAAATAAG+GGG + chr1_3:57670562-57670581 Msa0128020:CDS 25.0%
! AGCATATGCACAAAAAAAAA+TGG - chr1_3:57670881-57670900 None:intergenic 25.0%
!!! TTTTGTGCATATGCTATTTT+TGG + chr1_3:57670886-57670905 Msa0128020:three_prime_UTR 25.0%
! GATTCTGTTAACTTTCCTTT+TGG + chr1_3:57669869-57669888 Msa0128020:intron 30.0%
! TTTTCCTGAGAAAATATTGG+TGG + chr1_3:57670199-57670218 Msa0128020:intron 30.0%
CAAGTAAAGTCTTAACTCAA+AGG - chr1_3:57670257-57670276 None:intergenic 30.0%
TTTCTTTAACTACCAATGTG+TGG - chr1_3:57670308-57670327 None:intergenic 30.0%
TATATGGTCTCAATTAGAAG+AGG + chr1_3:57670338-57670357 Msa0128020:CDS 30.0%
ATATGGTCTCAATTAGAAGA+GGG + chr1_3:57670339-57670358 Msa0128020:CDS 30.0%
ATGAGTCAAAGAAATCATCT+CGG - chr1_3:57670398-57670417 None:intergenic 30.0%
TGATTTCTTTGACTCATTCA+TGG + chr1_3:57670401-57670420 Msa0128020:CDS 30.0%
! GATTCTGTTAACTTTCCTTT+TGG + chr1_3:57669869-57669888 Msa0128020:intron 30.0%
! TTTTCCTGAGAAAATATTGG+TGG + chr1_3:57670199-57670218 Msa0128020:intron 30.0%
CAAGTAAAGTCTTAACTCAA+AGG - chr1_3:57670257-57670276 None:intergenic 30.0%
TTTCTTTAACTACCAATGTG+TGG - chr1_3:57670308-57670327 None:intergenic 30.0%
TATATGGTCTCAATTAGAAG+AGG + chr1_3:57670338-57670357 Msa0128020:CDS 30.0%
ATATGGTCTCAATTAGAAGA+GGG + chr1_3:57670339-57670358 Msa0128020:CDS 30.0%
ATGAGTCAAAGAAATCATCT+CGG - chr1_3:57670398-57670417 None:intergenic 30.0%
TGATTTCTTTGACTCATTCA+TGG + chr1_3:57670401-57670420 Msa0128020:CDS 30.0%
CAATAATTCCAGATGGTGAT+GGG + chr1_3:57669786-57669805 Msa0128020:CDS 35.0%
! GCATTGTTACTTGATCCAAA+AGG - chr1_3:57669887-57669906 None:intergenic 35.0%
GTTGCTGAACATGCTATATA+TGG + chr1_3:57670055-57670074 Msa0128020:intron 35.0%
GTAGATTGAGTCAAAGTTGA+AGG + chr1_3:57670626-57670645 Msa0128020:CDS 35.0%
TACAAGGCTTAAGCAACAAT+TGG + chr1_3:57670656-57670675 Msa0128020:three_prime_UTR 35.0%
ACTTGTCATGATGCATGTTA+TGG - chr1_3:57670727-57670746 None:intergenic 35.0%
CAATAATTCCAGATGGTGAT+GGG + chr1_3:57669786-57669805 Msa0128020:CDS 35.0%
! GCATTGTTACTTGATCCAAA+AGG - chr1_3:57669887-57669906 None:intergenic 35.0%
GTTGCTGAACATGCTATATA+TGG + chr1_3:57670055-57670074 Msa0128020:intron 35.0%
GTAGATTGAGTCAAAGTTGA+AGG + chr1_3:57670626-57670645 Msa0128020:CDS 35.0%
TACAAGGCTTAAGCAACAAT+TGG + chr1_3:57670656-57670675 Msa0128020:three_prime_UTR 35.0%
ACTTGTCATGATGCATGTTA+TGG - chr1_3:57670727-57670746 None:intergenic 35.0%
TGCACATAGTAGTTCAGTTG+TGG + chr1_3:57669660-57669679 Msa0128020:five_prime_UTR 40.0%
CATAGTAGTTCAGTTGTGGT+TGG + chr1_3:57669664-57669683 Msa0128020:five_prime_UTR 40.0%
CTTGAAATGTACACATTGCG+AGG - chr1_3:57669700-57669719 None:intergenic 40.0%
! CCATCACCATCTGGAATTAT+TGG - chr1_3:57669788-57669807 None:intergenic 40.0%
CCAATAATTCCAGATGGTGA+TGG + chr1_3:57669785-57669804 Msa0128020:CDS 40.0%
!! ATCTCTAAGGCTTTGGAACA+TGG + chr1_3:57670165-57670184 Msa0128020:intron 40.0%
! TGCACCACCAATATTTTCTC+AGG - chr1_3:57670206-57670225 None:intergenic 40.0%
GTGCAAGTAGTTAGTTGTGT+TGG + chr1_3:57670221-57670240 Msa0128020:intron 40.0%
CCTGATAATTGCTTCCATGA+CGG - chr1_3:57670369-57670388 None:intergenic 40.0%
! CTCAGTTGATGCAAGAAGTT+CGG + chr1_3:57670599-57670618 Msa0128020:CDS 40.0%
GATTGAGTCAAAGTTGAAGG+AGG + chr1_3:57670629-57670648 Msa0128020:exon 40.0%
TGCACATAGTAGTTCAGTTG+TGG + chr1_3:57669660-57669679 Msa0128020:five_prime_UTR 40.0%
CATAGTAGTTCAGTTGTGGT+TGG + chr1_3:57669664-57669683 Msa0128020:five_prime_UTR 40.0%
CTTGAAATGTACACATTGCG+AGG - chr1_3:57669700-57669719 None:intergenic 40.0%
! CCATCACCATCTGGAATTAT+TGG - chr1_3:57669788-57669807 None:intergenic 40.0%
CCAATAATTCCAGATGGTGA+TGG + chr1_3:57669785-57669804 Msa0128020:CDS 40.0%
!! ATCTCTAAGGCTTTGGAACA+TGG + chr1_3:57670165-57670184 Msa0128020:intron 40.0%
! TGCACCACCAATATTTTCTC+AGG - chr1_3:57670206-57670225 None:intergenic 40.0%
GTGCAAGTAGTTAGTTGTGT+TGG + chr1_3:57670221-57670240 Msa0128020:intron 40.0%
CCTGATAATTGCTTCCATGA+CGG - chr1_3:57670369-57670388 None:intergenic 40.0%
! CTCAGTTGATGCAAGAAGTT+CGG + chr1_3:57670599-57670618 Msa0128020:CDS 40.0%
GATTGAGTCAAAGTTGAAGG+AGG + chr1_3:57670629-57670648 Msa0128020:exon 40.0%
! CTAACATGGCTAGCTGAAGA+TGG - chr1_3:57669740-57669759 None:intergenic 45.0%
CAACGGTGTCTCTACTAACA+TGG - chr1_3:57669754-57669773 None:intergenic 45.0%
CATGTTAGTAGAGACACCGT+TGG + chr1_3:57669752-57669771 Msa0128020:CDS 45.0%
ATGCAAGACTGTTGTGCTTG+TGG + chr1_3:57669903-57669922 Msa0128020:intron 45.0%
!! CTAAGGCTTTGGAACATGGT+TGG + chr1_3:57670169-57670188 Msa0128020:intron 45.0%
CAGGTTTGATCACCACACAT+TGG + chr1_3:57670293-57670312 Msa0128020:intron 45.0%
TAGAAGAGGGAAAACCGTCA+TGG + chr1_3:57670352-57670371 Msa0128020:CDS 45.0%
CCGTCATGGAAGCAATTATC+AGG + chr1_3:57670366-57670385 Msa0128020:CDS 45.0%
TACATGGCAACCAGAGCATA+AGG + chr1_3:57670531-57670550 Msa0128020:CDS 45.0%
! CGCACTTTTACCTTATGCTC+TGG - chr1_3:57670544-57670563 None:intergenic 45.0%
AGTTGAAGGAGGCAACTACA+AGG + chr1_3:57670640-57670659 Msa0128020:exon 45.0%
! CTAACATGGCTAGCTGAAGA+TGG - chr1_3:57669740-57669759 None:intergenic 45.0%
CAACGGTGTCTCTACTAACA+TGG - chr1_3:57669754-57669773 None:intergenic 45.0%
CATGTTAGTAGAGACACCGT+TGG + chr1_3:57669752-57669771 Msa0128020:CDS 45.0%
ATGCAAGACTGTTGTGCTTG+TGG + chr1_3:57669903-57669922 Msa0128020:intron 45.0%
!! CTAAGGCTTTGGAACATGGT+TGG + chr1_3:57670169-57670188 Msa0128020:intron 45.0%
CAGGTTTGATCACCACACAT+TGG + chr1_3:57670293-57670312 Msa0128020:intron 45.0%
TAGAAGAGGGAAAACCGTCA+TGG + chr1_3:57670352-57670371 Msa0128020:CDS 45.0%
CCGTCATGGAAGCAATTATC+AGG + chr1_3:57670366-57670385 Msa0128020:CDS 45.0%
TACATGGCAACCAGAGCATA+AGG + chr1_3:57670531-57670550 Msa0128020:CDS 45.0%
! CGCACTTTTACCTTATGCTC+TGG - chr1_3:57670544-57670563 None:intergenic 45.0%
AGTTGAAGGAGGCAACTACA+AGG + chr1_3:57670640-57670659 Msa0128020:exon 45.0%
CAAACTCACCCATCACCATC+TGG - chr1_3:57669797-57669816 None:intergenic 50.0%
CAAACTCACCCATCACCATC+TGG - chr1_3:57669797-57669816 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 57669598 57670934 57669598 ID=Msa0128020;Name=Msa0128020
chr1_3 mRNA 57669598 57670934 57669598 ID=Msa0128020-mRNA-1;Parent=Msa0128020;Name=Msa0128020-mRNA-1;_AED=0.28;_eAED=0.28;_QI=106|1|1|1|1|1|3|289|117
chr1_3 exon 57669598 57669807 57669598 ID=Msa0128020-mRNA-1:exon:18337;Parent=Msa0128020-mRNA-1
chr1_3 exon 57670296 57670422 57670296 ID=Msa0128020-mRNA-1:exon:18338;Parent=Msa0128020-mRNA-1
chr1_3 exon 57670523 57670934 57670523 ID=Msa0128020-mRNA-1:exon:18339;Parent=Msa0128020-mRNA-1
chr1_3 five_prime_UTR 57669598 57669703 57669598 ID=Msa0128020-mRNA-1:five_prime_utr;Parent=Msa0128020-mRNA-1
chr1_3 CDS 57669704 57669807 57669704 ID=Msa0128020-mRNA-1:cds;Parent=Msa0128020-mRNA-1
chr1_3 CDS 57670296 57670422 57670296 ID=Msa0128020-mRNA-1:cds;Parent=Msa0128020-mRNA-1
chr1_3 CDS 57670523 57670645 57670523 ID=Msa0128020-mRNA-1:cds;Parent=Msa0128020-mRNA-1
chr1_3 three_prime_UTR 57670646 57670934 57670646 ID=Msa0128020-mRNA-1:three_prime_utr;Parent=Msa0128020-mRNA-1
Gene Sequence

>Msa0128020

ATGTGTACATTTCAAGTTATGGTCAATAGAGCTCCATCTTCAGCTAGCCATGTTAGTAGAGACACCGTTGGTACTAAAATACCAATAATTCCAGATGGTGATGGGTTTGATCACCACACATTGGTAGTTAAAGAAATTGCTCACATTATATGGTCTCAATTAGAAGAGGGAAAACCGTCATGGAAGCAATTATCAGGAAATCACCGAGATGATTTCTTTGACTCATTCATGAAAAAGTTTACATGGCAACCAGAGCATAAGGTAAAAGTGCGAAAAAACTTTGAAATAAGGGGTGCAGCTAAGATGTCTCAGTTGATGCAAGAAGTTCGGAAAAGTAGATTGAGTCAAAGTTGA

Protein sequence

>Msa0128020

MCTFQVMVNRAPSSASHVSRDTVGTKIPIIPDGDGFDHHTLVVKEIAHIIWSQLEEGKPSWKQLSGNHRDDFFDSFMKKFTWQPEHKVKVRKNFEIRGAAKMSQLMQEVRKSRLSQS*