AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0594710


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0594710 MtrunA17_Chr1g0182201 80.435 92 2 1 40 115 1 92 8.49e-45 140
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0594710 AT1G33500.2 46.296 108 42 2 18 109 147 254 1.34e-22 89.0
Msa0594710 AT1G33500.3 46.296 108 42 2 18 109 147 254 1.34e-22 89.0
Msa0594710 AT1G33500.1 44.944 89 33 2 18 90 147 235 2.87e-14 67.0

Find 17 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCCATAGACTGCAAGTATT+CGG 0.336412 4_2:+22914349 None:intergenic
GTAAAATACCAGGTTATCTA+TGG 0.364353 4_2:+22915254 None:intergenic
AACCGAATACTTGCAGTCTA+TGG 0.365243 4_2:-22914351 Msa0594710:CDS
GAACAACTTAATCACTGAGT+TGG 0.468913 4_2:-22915157 Msa0594710:CDS
TATGAAATCCAAGGTTCTCA+TGG 0.508723 4_2:-22915100 Msa0594710:CDS
GTTAATGTCCATGAGAACCT+TGG 0.514632 4_2:+22915092 None:intergenic
CAGTCTATGGAGAAACAAGT+AGG 0.519458 4_2:-22914338 Msa0594710:CDS
TGATATAACCGCTCTTGAAG+AGG 0.585407 4_2:-22914408 Msa0594710:CDS
TAACTGTGCCATAGATAACC+TGG 0.597968 4_2:-22915262 Msa0594710:intron
AGTCTATGGAGAAACAAGTA+GGG 0.599025 4_2:-22914337 Msa0594710:CDS
GATTCTTGTTATGAAATCCA+AGG 0.599940 4_2:-22915109 Msa0594710:CDS
AGTTGGATTCAGCTAAAGCA+AGG 0.606877 4_2:-22915140 Msa0594710:CDS
ATTATATTCCTCTTCAAGAG+CGG 0.617481 4_2:+22914400 None:intergenic
CAACTTATAAAATGTGCTTG+TGG 0.646559 4_2:-22914091 Msa0594710:CDS
TATAAAATGTGCTTGTGGTG+AGG 0.649465 4_2:-22914086 Msa0594710:CDS
GAAACAAGTAGGGAAACTCA+AGG 0.667054 4_2:-22914327 Msa0594710:intron
GGTTCTCATGGACATTAACA+AGG 0.678444 4_2:-22915088 Msa0594710:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCAATTTAGTTTCATAAAA+CGG + chr4_2:22914087-22914106 None:intergenic 15.0%
!!! TATGTTAATATTGTTGTTTA+TGG - chr4_2:22914585-22914604 Msa0594710:intron 15.0%
!! AAAGTAATTATCAACAAAAT+AGG - chr4_2:22915159-22915178 Msa0594710:CDS 15.0%
!!! ATCAATTTAGTTTCATAAAA+CGG + chr4_2:22914087-22914106 None:intergenic 15.0%
!!! TATGTTAATATTGTTGTTTA+TGG - chr4_2:22914585-22914604 Msa0594710:intron 15.0%
!! AAAGTAATTATCAACAAAAT+AGG - chr4_2:22915159-22915178 Msa0594710:CDS 15.0%
!! AAGTTTATTATACGAATGTT+TGG + chr4_2:22914112-22914131 None:intergenic 20.0%
!! TATAATAAACTTATCTTGCA+GGG - chr4_2:22914120-22914139 Msa0594710:intron 20.0%
!! TAATTTATCTTTGTCTGATA+GGG - chr4_2:22914230-22914249 Msa0594710:intron 20.0%
!! TTATTCATTACTCAAGTTAT+TGG - chr4_2:22914387-22914406 Msa0594710:CDS 20.0%
!! TAAAATAATTGTCTTCAGAA+TGG - chr4_2:22914537-22914556 Msa0594710:intron 20.0%
!! AAAATAATTGTCTTCAGAAT+GGG - chr4_2:22914538-22914557 Msa0594710:intron 20.0%
!!! TAACTTGTAAAATTTTCCAT+TGG + chr4_2:22915136-22915155 None:intergenic 20.0%
!! AAGTTTATTATACGAATGTT+TGG + chr4_2:22914112-22914131 None:intergenic 20.0%
!! TATAATAAACTTATCTTGCA+GGG - chr4_2:22914120-22914139 Msa0594710:intron 20.0%
!! TAATTTATCTTTGTCTGATA+GGG - chr4_2:22914230-22914249 Msa0594710:intron 20.0%
!! TTATTCATTACTCAAGTTAT+TGG - chr4_2:22914387-22914406 Msa0594710:CDS 20.0%
!! TAAAATAATTGTCTTCAGAA+TGG - chr4_2:22914537-22914556 Msa0594710:intron 20.0%
!! AAAATAATTGTCTTCAGAAT+GGG - chr4_2:22914538-22914557 Msa0594710:intron 20.0%
!!! TAACTTGTAAAATTTTCCAT+TGG + chr4_2:22915136-22915155 None:intergenic 20.0%
! GTATAATAAACTTATCTTGC+AGG - chr4_2:22914119-22914138 Msa0594710:intron 25.0%
! GTAATTTATCTTTGTCTGAT+AGG - chr4_2:22914229-22914248 Msa0594710:intron 25.0%
! AATTTATCTTTGTCTGATAG+GGG - chr4_2:22914231-22914250 Msa0594710:intron 25.0%
! GAATAATTGACAGTCTAAAA+AGG + chr4_2:22914373-22914392 None:intergenic 25.0%
! TTCAGAAAATACAGATGAAA+TGG + chr4_2:22914431-22914450 None:intergenic 25.0%
! AAATAATTGTCTTCAGAATG+GGG - chr4_2:22914539-22914558 Msa0594710:intron 25.0%
!! ATATATGCTTCTGATTTTAC+AGG - chr4_2:22915300-22915319 Msa0594710:intron 25.0%
! GTATAATAAACTTATCTTGC+AGG - chr4_2:22914119-22914138 Msa0594710:intron 25.0%
! GTAATTTATCTTTGTCTGAT+AGG - chr4_2:22914229-22914248 Msa0594710:intron 25.0%
! AATTTATCTTTGTCTGATAG+GGG - chr4_2:22914231-22914250 Msa0594710:intron 25.0%
! GAATAATTGACAGTCTAAAA+AGG + chr4_2:22914373-22914392 None:intergenic 25.0%
! TTCAGAAAATACAGATGAAA+TGG + chr4_2:22914431-22914450 None:intergenic 25.0%
! AAATAATTGTCTTCAGAATG+GGG - chr4_2:22914539-22914558 Msa0594710:intron 25.0%
!! ATATATGCTTCTGATTTTAC+AGG - chr4_2:22915300-22915319 Msa0594710:intron 25.0%
GTAAAATACCAGGTTATCTA+TGG + chr4_2:22914171-22914190 None:intergenic 30.0%
GATGAAAAAGGTAAAATACC+AGG + chr4_2:22914181-22914200 None:intergenic 30.0%
GAAGAACAGATTGATGAAAA+AGG + chr4_2:22914193-22914212 None:intergenic 30.0%
! GATTCTTGTTATGAAATCCA+AGG - chr4_2:22914313-22914332 Msa0594710:intron 30.0%
! GTATTTTCTGAATTACATGC+TGG - chr4_2:22914439-22914458 Msa0594710:intron 30.0%
!! CTAGATAAAACTTGCTATTG+AGG - chr4_2:22914651-22914670 Msa0594710:intron 30.0%
!!! GATTTAGCTTTTCTCTTAAC+TGG - chr4_2:22914888-22914907 Msa0594710:intron 30.0%
ATTATATTCCTCTTCAAGAG+CGG + chr4_2:22915025-22915044 None:intergenic 30.0%
GTAAAATAGGTTTAGTCCAA+TGG - chr4_2:22915117-22915136 Msa0594710:CDS 30.0%
AGCATATATCCATGATTGAA+GGG + chr4_2:22915289-22915308 None:intergenic 30.0%
AAGCATATATCCATGATTGA+AGG + chr4_2:22915290-22915309 None:intergenic 30.0%
CAACTTATAAAATGTGCTTG+TGG - chr4_2:22915331-22915350 Msa0594710:intron 30.0%
GTAAAATACCAGGTTATCTA+TGG + chr4_2:22914171-22914190 None:intergenic 30.0%
GATGAAAAAGGTAAAATACC+AGG + chr4_2:22914181-22914200 None:intergenic 30.0%
GAAGAACAGATTGATGAAAA+AGG + chr4_2:22914193-22914212 None:intergenic 30.0%
! GATTCTTGTTATGAAATCCA+AGG - chr4_2:22914313-22914332 Msa0594710:intron 30.0%
! GTATTTTCTGAATTACATGC+TGG - chr4_2:22914439-22914458 Msa0594710:intron 30.0%
!! CTAGATAAAACTTGCTATTG+AGG - chr4_2:22914651-22914670 Msa0594710:intron 30.0%
!!! GATTTAGCTTTTCTCTTAAC+TGG - chr4_2:22914888-22914907 Msa0594710:intron 30.0%
ATTATATTCCTCTTCAAGAG+CGG + chr4_2:22915025-22915044 None:intergenic 30.0%
GTAAAATAGGTTTAGTCCAA+TGG - chr4_2:22915117-22915136 Msa0594710:CDS 30.0%
AGCATATATCCATGATTGAA+GGG + chr4_2:22915289-22915308 None:intergenic 30.0%
AAGCATATATCCATGATTGA+AGG + chr4_2:22915290-22915309 None:intergenic 30.0%
CAACTTATAAAATGTGCTTG+TGG - chr4_2:22915331-22915350 Msa0594710:intron 30.0%
AATAAACTTATCTTGCAGGG+AGG - chr4_2:22914123-22914142 Msa0594710:intron 35.0%
CAATCTGTTCTTCACAAAGT+TGG - chr4_2:22914200-22914219 Msa0594710:intron 35.0%
TTCTTCACAAAGTTGGAATC+TGG - chr4_2:22914207-22914226 Msa0594710:intron 35.0%
GAACAACTTAATCACTGAGT+TGG - chr4_2:22914265-22914284 Msa0594710:intron 35.0%
TATGAAATCCAAGGTTCTCA+TGG - chr4_2:22914322-22914341 Msa0594710:intron 35.0%
CATCGTATTTACCATGATCA+AGG - chr4_2:22914477-22914496 Msa0594710:intron 35.0%
GTGCAGAGAAAATGAGTTTA+AGG - chr4_2:22914681-22914700 Msa0594710:intron 35.0%
AGTCTATGGAGAAACAAGTA+GGG - chr4_2:22915085-22915104 Msa0594710:intron 35.0%
AGGGAAACTCAAGGTAAAAT+AGG - chr4_2:22915104-22915123 Msa0594710:CDS 35.0%
! CATCTGTTCGACTTTTACAT+AGG + chr4_2:22915254-22915273 None:intergenic 35.0%
ATATATCCATGATTGAAGGG+GGG + chr4_2:22915286-22915305 None:intergenic 35.0%
CATATATCCATGATTGAAGG+GGG + chr4_2:22915287-22915306 None:intergenic 35.0%
GCATATATCCATGATTGAAG+GGG + chr4_2:22915288-22915307 None:intergenic 35.0%
TATAAAATGTGCTTGTGGTG+AGG - chr4_2:22915336-22915355 Msa0594710:intron 35.0%
AATAAACTTATCTTGCAGGG+AGG - chr4_2:22914123-22914142 Msa0594710:intron 35.0%
CAATCTGTTCTTCACAAAGT+TGG - chr4_2:22914200-22914219 Msa0594710:intron 35.0%
TTCTTCACAAAGTTGGAATC+TGG - chr4_2:22914207-22914226 Msa0594710:intron 35.0%
GAACAACTTAATCACTGAGT+TGG - chr4_2:22914265-22914284 Msa0594710:intron 35.0%
TATGAAATCCAAGGTTCTCA+TGG - chr4_2:22914322-22914341 Msa0594710:intron 35.0%
CATCGTATTTACCATGATCA+AGG - chr4_2:22914477-22914496 Msa0594710:intron 35.0%
GTGCAGAGAAAATGAGTTTA+AGG - chr4_2:22914681-22914700 Msa0594710:intron 35.0%
AGTCTATGGAGAAACAAGTA+GGG - chr4_2:22915085-22915104 Msa0594710:intron 35.0%
AGGGAAACTCAAGGTAAAAT+AGG - chr4_2:22915104-22915123 Msa0594710:CDS 35.0%
! CATCTGTTCGACTTTTACAT+AGG + chr4_2:22915254-22915273 None:intergenic 35.0%
ATATATCCATGATTGAAGGG+GGG + chr4_2:22915286-22915305 None:intergenic 35.0%
CATATATCCATGATTGAAGG+GGG + chr4_2:22915287-22915306 None:intergenic 35.0%
GCATATATCCATGATTGAAG+GGG + chr4_2:22915288-22915307 None:intergenic 35.0%
TATAAAATGTGCTTGTGGTG+AGG - chr4_2:22915336-22915355 Msa0594710:intron 35.0%
TAACTGTGCCATAGATAACC+TGG - chr4_2:22914160-22914179 Msa0594710:intron 40.0%
AGTTGGATTCAGCTAAAGCA+AGG - chr4_2:22914282-22914301 Msa0594710:intron 40.0%
GTTAATGTCCATGAGAACCT+TGG + chr4_2:22914333-22914352 None:intergenic 40.0%
GGTTCTCATGGACATTAACA+AGG - chr4_2:22914334-22914353 Msa0594710:CDS 40.0%
CATGCACGTATATAGAGAGA+TGG - chr4_2:22914750-22914769 Msa0594710:intron 40.0%
CTCAGGCTAGATCATTCATT+TGG + chr4_2:22914983-22915002 None:intergenic 40.0%
!!! ATATCAGCTGCTTTTAGCTC+AGG + chr4_2:22915000-22915019 None:intergenic 40.0%
TGATATAACCGCTCTTGAAG+AGG - chr4_2:22915014-22915033 Msa0594710:intron 40.0%
AACCGAATACTTGCAGTCTA+TGG - chr4_2:22915071-22915090 Msa0594710:intron 40.0%
CTCCATAGACTGCAAGTATT+CGG + chr4_2:22915076-22915095 None:intergenic 40.0%
CAGTCTATGGAGAAACAAGT+AGG - chr4_2:22915084-22915103 Msa0594710:intron 40.0%
GAAACAAGTAGGGAAACTCA+AGG - chr4_2:22915095-22915114 Msa0594710:CDS 40.0%
TAACTGTGCCATAGATAACC+TGG - chr4_2:22914160-22914179 Msa0594710:intron 40.0%
AGTTGGATTCAGCTAAAGCA+AGG - chr4_2:22914282-22914301 Msa0594710:intron 40.0%
GTTAATGTCCATGAGAACCT+TGG + chr4_2:22914333-22914352 None:intergenic 40.0%
GGTTCTCATGGACATTAACA+AGG - chr4_2:22914334-22914353 Msa0594710:CDS 40.0%
CATGCACGTATATAGAGAGA+TGG - chr4_2:22914750-22914769 Msa0594710:intron 40.0%
CTCAGGCTAGATCATTCATT+TGG + chr4_2:22914983-22915002 None:intergenic 40.0%
!!! ATATCAGCTGCTTTTAGCTC+AGG + chr4_2:22915000-22915019 None:intergenic 40.0%
TGATATAACCGCTCTTGAAG+AGG - chr4_2:22915014-22915033 Msa0594710:intron 40.0%
AACCGAATACTTGCAGTCTA+TGG - chr4_2:22915071-22915090 Msa0594710:intron 40.0%
CTCCATAGACTGCAAGTATT+CGG + chr4_2:22915076-22915095 None:intergenic 40.0%
CAGTCTATGGAGAAACAAGT+AGG - chr4_2:22915084-22915103 Msa0594710:intron 40.0%
GAAACAAGTAGGGAAACTCA+AGG - chr4_2:22915095-22915114 Msa0594710:CDS 40.0%
ACATGCTGGAATGTGTGCAT+TGG - chr4_2:22914453-22914472 Msa0594710:intron 45.0%
CATGCTGGAATGTGTGCATT+GGG - chr4_2:22914454-22914473 Msa0594710:intron 45.0%
GCAGTGTTCAACCTTGATCA+TGG + chr4_2:22914491-22914510 None:intergenic 45.0%
! GCTCTTTTGTCTGACAAAGC+TGG - chr4_2:22915045-22915064 Msa0594710:intron 45.0%
TAGTTTCCCCCCTTCAATCA+TGG - chr4_2:22915277-22915296 Msa0594710:CDS 45.0%
ACATGCTGGAATGTGTGCAT+TGG - chr4_2:22914453-22914472 Msa0594710:intron 45.0%
CATGCTGGAATGTGTGCATT+GGG - chr4_2:22914454-22914473 Msa0594710:intron 45.0%
GCAGTGTTCAACCTTGATCA+TGG + chr4_2:22914491-22914510 None:intergenic 45.0%
! GCTCTTTTGTCTGACAAAGC+TGG - chr4_2:22915045-22915064 Msa0594710:intron 45.0%
TAGTTTCCCCCCTTCAATCA+TGG - chr4_2:22915277-22915296 Msa0594710:CDS 45.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 22914048 22915396 22914048 ID=Msa0594710;Name=Msa0594710
chr4_2 mRNA 22914048 22915396 22914048 ID=Msa0594710-mRNA-1;Parent=Msa0594710;Name=Msa0594710-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0.25|0.2|0.8|0.75|0.6|5|0|115
chr4_2 exon 22915393 22915396 22915393 ID=Msa0594710-mRNA-1:exon:6241;Parent=Msa0594710-mRNA-1
chr4_2 exon 22915263 22915303 22915263 ID=Msa0594710-mRNA-1:exon:6240;Parent=Msa0594710-mRNA-1
chr4_2 exon 22915089 22915193 22915089 ID=Msa0594710-mRNA-1:exon:6239;Parent=Msa0594710-mRNA-1
chr4_2 exon 22914328 22914447 22914328 ID=Msa0594710-mRNA-1:exon:6238;Parent=Msa0594710-mRNA-1
chr4_2 exon 22914048 22914122 22914048 ID=Msa0594710-mRNA-1:exon:6237;Parent=Msa0594710-mRNA-1
chr4_2 CDS 22915393 22915396 22915393 ID=Msa0594710-mRNA-1:cds;Parent=Msa0594710-mRNA-1
chr4_2 CDS 22915263 22915303 22915263 ID=Msa0594710-mRNA-1:cds;Parent=Msa0594710-mRNA-1
chr4_2 CDS 22915089 22915193 22915089 ID=Msa0594710-mRNA-1:cds;Parent=Msa0594710-mRNA-1
chr4_2 CDS 22914328 22914447 22914328 ID=Msa0594710-mRNA-1:cds;Parent=Msa0594710-mRNA-1
chr4_2 CDS 22914048 22914122 22914048 ID=Msa0594710-mRNA-1:cds;Parent=Msa0594710-mRNA-1
Gene Sequence

>Msa0594710

TCTGGGAGGACTAGTGCTGATGATAACTGTGCCATAGATAACCTGGGGAGTGATGCAAAGAACAACTTAATCACTGAGTTGGATTCAGCTAAAGCAAGGCTTGATGAGATTCTTGTTATGAAATCCAAGGTTCTCATGGACATTAACAAGCCTGAGCTAAAAGCAGCTGATATAACCGCTCTTGAAGAGGAATATAATGCTCTTTTGTCTGACAAAGCTGGAGAAACCGAATACTTGCAGTCTATGGAGAAACAAGTAGGGAAACTCAAGGAGATCAATCAACTTATAAAATGTGCTTGTGGTGAGGAATACAAAGTTGCATTGAATACACAGTTGCAGTGTACT

Protein sequence

>Msa0594710

SGRTSADDNCAIDNLGSDAKNNLITELDSAKARLDEILVMKSKVLMDINKPELKAADITALEEEYNALLSDKAGETEYLQSMEKQVGKLKEINQLIKCACGEEYKVALNTQLQCT