AlfalfaGEDB | MS.gene91973

M. sativa cultivar XinJiangDaYe / MS.gene91973

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0055114
PFAM: PF00248.24

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91973.t1	AAB41556.1	99.4	312	2	0	1	312	1	312	3.40E-176	627.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91973.t1	P26690	84.8	310	47	0	3	312	4	313	1.1e-154	547.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91973.t1	Q40310	99.4	312	2	0	1	312	1	312	2.4e-176	627.5

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049222	MS.gene91973	-0.811535	6.67E-51	-1.69E-46
MS.gene049232	MS.gene91973	-0.834665	2.63E-56	-1.69E-46
MS.gene049320	MS.gene91973	-0.811944	5.43E-51	-1.69E-46
MS.gene049483	MS.gene91973	0.808595	2.87E-50	-1.69E-46
MS.gene049611	MS.gene91973	0.812042	5.17E-51	-1.69E-46
MS.gene049634	MS.gene91973	0.802649	5.08E-49	-1.69E-46
MS.gene050095	MS.gene91973	0.805237	1.47E-49	-1.69E-46
MS.gene05010	MS.gene91973	-0.809986	1.44E-50	-1.69E-46
MS.gene050129	MS.gene91973	0.800159	1.64E-48	-1.69E-46
MS.gene050216	MS.gene91973	-0.836144	1.11E-56	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene040151	MS.gene91973	PPI
MS.gene91973	MS.gene42873	PPI
MS.gene91973	MS.gene87111	PPI
MS.gene91973	MS.gene62620	PPI
MS.gene91973	MS.gene97980	PPI
MS.gene91973	MS.gene97981	PPI
MS.gene91973	MS.gene056487	PPI
MS.gene91973	MS.gene062932	PPI
MS.gene91973	MS.gene040151	PPI
MS.gene91973	MS.gene50700	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91973.t1	MTR_5g097900	98.397	312	5	0	1	312	1	312	0.0	637
MS.gene91973.t1	MTR_5g097910	98.077	312	6	0	1	312	1	312	0.0	636
MS.gene91973.t1	MTR_5g097940	98.077	312	6	0	1	312	1	312	0.0	636
MS.gene91973.t1	MTR_3g083130	68.182	308	97	1	6	312	7	314	7.27e-162	453
MS.gene91973.t1	MTR_0374s0050	62.951	305	110	3	1	302	1	305	1.55e-137	392
MS.gene91973.t1	MTR_4g072320	52.508	299	132	4	1	297	1	291	3.76e-101	300
MS.gene91973.t1	MTR_4g072350	51.155	303	136	5	20	311	17	318	3.72e-99	295
MS.gene91973.t1	MTR_4g072360	50.000	300	144	4	1	297	1	297	8.33e-99	294
MS.gene91973.t1	MTR_6g073110	50.165	303	145	4	1	300	1	300	1.04e-97	291
MS.gene91973.t1	MTR_8g070095	42.958	284	159	2	19	300	15	297	3.07e-75	233

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91973.t1	AT1G59960	53.571	308	132	6	1	300	1	305	6.85e-112	327
MS.gene91973.t1	AT1G59950	55.903	288	117	5	20	300	15	299	1.20e-104	308
MS.gene91973.t1	AT2G37770	44.755	286	147	4	19	300	15	293	1.13e-77	239
MS.gene91973.t1	AT2G37790	43.357	286	151	4	19	300	15	293	2.10e-76	236
MS.gene91973.t1	AT5G62420	43.662	284	154	4	20	300	14	294	1.09e-73	229
MS.gene91973.t1	AT5G01670	42.254	284	155	6	19	300	23	299	1.10e-72	227
MS.gene91973.t1	AT2G37760	43.662	284	147	4	19	299	15	288	4.11e-72	224
MS.gene91973.t1	AT2G37760	43.662	284	147	4	19	299	15	288	4.11e-72	224
MS.gene91973.t1	AT2G37760	44.128	281	144	4	19	296	15	285	2.94e-71	223
MS.gene91973.t1	AT2G37760	43.617	282	146	4	19	297	15	286	2.34e-70	221

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Find 67 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTTGTGTATCTTTGAT+TGG	0.361005	5.2:+82463620	MS.gene91973:CDS
AAAGAGATTGCAGATGCTCA+TGG	0.540234	5.2:+82463508	MS.gene91973:CDS
AAAGTTTGGAAGTAACAAAA+AGG	0.467615	5.2:-82462724	None:intergenic
AAGAGATCTGCCACATCAAT+TGG	0.378807	5.2:-82463109	None:intergenic
AAGAGCTTGTTCTGAACCAT+AGG	0.526693	5.2:-82462651	None:intergenic
AAGATGCCTGTGGTTGGAAT+GGG	0.555328	5.2:+82462536	MS.gene91973:CDS
AAGCAAAATCGTTTGATCCC+TGG	0.455139	5.2:+82463682	MS.gene91973:CDS
AAGTTTCAAGCTTTCCTCCA+TGG	0.483539	5.2:-82463161	None:intergenic
AATCAAAGATACACAAATTT+TGG	0.147232	5.2:-82463618	None:intergenic
AGAGCAGGAATAACAAGATG+AGG	0.624425	5.2:-82462764	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTGGAAATGAAAACTTTCC+AGG	-	chr5.2:82463094-82463113	None:intergenic	30.0%
CTTTCTTACATGTGAAATCA+GGG	-	chr5.2:82462568-82462587	None:intergenic	30.0%
TCTTTCTTACATGTGAAATC+AGG	-	chr5.2:82462569-82462588	None:intergenic	30.0%
TTTCTTACATGTGAAATCAG+GGG	-	chr5.2:82462567-82462586	None:intergenic	30.0%
ATCATTGAAGCCATCAAACA+AGG	+	chr5.2:82462602-82462621	MS.gene91973:CDS	35.0%
CAAACACCTTTCACATCAAA+TGG	-	chr5.2:82463136-82463155	None:intergenic	35.0%
CTAAGATGGTTATACGAACA+AGG	+	chr5.2:82463553-82463572	MS.gene91973:CDS	35.0%
CTCTTACCATTTGATGTGAA+AGG	+	chr5.2:82463127-82463146	MS.gene91973:CDS	35.0%
GTGTTTGTAAGAACCTTTGT+TGG	-	chr5.2:82462503-82462522	None:intergenic	35.0%
TCATTCTCCATAACCTCATT+AGG	-	chr5.2:82463481-82463500	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	82462482	82463744	82462482	ID=MS.gene91973
chr5.2	mRNA	82462482	82463744	82462482	ID=MS.gene91973.t1;Parent=MS.gene91973
chr5.2	exon	82462482	82462801	82462482	ID=MS.gene91973.t1.exon1;Parent=MS.gene91973.t1
chr5.2	CDS	82462482	82462801	82462482	ID=cds.MS.gene91973.t1;Parent=MS.gene91973.t1
chr5.2	exon	82463033	82463276	82463033	ID=MS.gene91973.t1.exon2;Parent=MS.gene91973.t1
chr5.2	CDS	82463033	82463276	82463033	ID=cds.MS.gene91973.t1;Parent=MS.gene91973.t1
chr5.2	exon	82463370	82463744	82463370	ID=MS.gene91973.t1.exon3;Parent=MS.gene91973.t1
chr5.2	CDS	82463370	82463744	82463370	ID=cds.MS.gene91973.t1;Parent=MS.gene91973.t1

Gene Sequence

>MS.gene91973

ATGGGTAGTGTTGAAATTCCAACAAAGGTTCTTACAAACACCTCTAGTCAATTGAAGATGCCTGTGGTTGGAATGGGATCAGCCCCTGATTTCACATGTAAGAAAGACACAAAAGATGCAATCATTGAAGCCATCAAACAAGGTTATAGACACTTTGATACTGCTGCTGCCTATGGTTCAGAACAAGCTCTTGGAGAGGCTTTGAAAGAGGCAATTGAACTTGGTCTTGTCACTAGAGAAGACCTTTTTGTTACTTCCAAACTTTGGGTCACTGAAAACCATCCTCATCTTGTTATTCCTGCTCTTCAAAAATCTCTCAAGTAAGCAAATTTTAATGTTACACTCATTTAGAGTATATTTAGATGACATTTGGTTCTATAGTGACGAAATTAATTTTTAATCAATTGATTTTGTAAAATATATTTGATTAAAAGTAAGATCGTTAAATTGTTTTTTATTTATATATATTCACGTAAATATCCATTGAAGAACAAAAATGAATCAAAACATACACTTATTGAGATTAAACATGATTTGTTGATTTCGTGCAGGACTCTTCAACTAGACTACTTGGACTTGTACTTGATTCATTGGCCACTTAGTTCTCAACCTGGAAAGTTTTCATTTCCAATTGATGTGGCAGATCTCTTACCATTTGATGTGAAAGGTGTTTGGGAATCCATGGAGGAAAGCTTGAAACTTGGACTCACTAAAGCTATTGGAGTTAGTAACTTCTCTGTCAAGAAACTTGAAAATCTTCTCTCTGTTGCCACTGTTCTTCCTGCAGTCAATCAAGTAAGTCCTCAATTAAACCTAAGTTTAGATTAACTTCTTGTAAACTTCTTAATTGTTAATATGTACTTATACAATGAACTCATATATTTATAGGTGGAGATGAACCTTGCATGGCAACAAAAGAAGCTTAGAGAGTTTTGCAACGCACACGGAATAGTGTTAACCGCATTTTCCCCGTTGAGGAAAGGTGCAAGCAGAGGACCTAATGAGGTTATGGAGAATGATATGCTTAAAGAGATTGCAGATGCTCATGGAAAGTCTGTTGCACAAATATCTCTAAGATGGTTATACGAACAAGGAGTCACTTTTGTTCCCAAGAGCTACGATAAGGAGAGAATGAACCAAAATTTGTGTATCTTTGATTGGTCATTGACAAAGGAGGATCATGAGAAGATTGATCAAATTAAGCAAAATCGTTTGATCCCTGGACCAACCAAGCCCGGACTCAATGACCTCTATGATGACTAA

Protein sequence

>MS.gene91973.t1

MGSVEIPTKVLTNTSSQLKMPVVGMGSAPDFTCKKDTKDAIIEAIKQGYRHFDTAAAYGSEQALGEALKEAIELGLVTREDLFVTSKLWVTENHPHLVIPALQKSLKTLQLDYLDLYLIHWPLSSQPGKFSFPIDVADLLPFDVKGVWESMEESLKLGLTKAIGVSNFSVKKLENLLSVATVLPAVNQVEMNLAWQQKKLREFCNAHGIVLTAFSPLRKGASRGPNEVMENDMLKEIADAHGKSVAQISLRWLYEQGVTFVPKSYDKERMNQNLCIFDWSLTKEDHEKIDQIKQNRLIPGPTKPGLNDLYDD