Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067108.t1 | KEH21484.1 | 98.8 | 323 | 4 | 0 | 2 | 324 | 12 | 334 | 6.00E-184 | 653.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067108.t1 | Q3UDW8 | 27.8 | 338 | 185 | 12 | 3 | 317 | 274 | 575 | 1.8e-19 | 98.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067108.t1 | A0A072TVF5 | 98.8 | 323 | 4 | 0 | 2 | 324 | 12 | 334 | 4.3e-184 | 653.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene067108 | 0.802882 | 4.54E-49 | -1.69E-46 |
| MS.gene049171 | MS.gene067108 | 0.834978 | 2.19E-56 | -1.69E-46 |
| MS.gene049222 | MS.gene067108 | 0.807477 | 4.96E-50 | -1.69E-46 |
| MS.gene049609 | MS.gene067108 | 0.800982 | 1.12E-48 | -1.69E-46 |
| MS.gene049633 | MS.gene067108 | 0.83748 | 5.07E-57 | -1.69E-46 |
| MS.gene050170 | MS.gene067108 | 0.810003 | 1.43E-50 | -1.69E-46 |
| MS.gene050338 | MS.gene067108 | -0.831229 | 1.88E-55 | -1.69E-46 |
| MS.gene050444 | MS.gene067108 | 0.826867 | 2.14E-54 | -1.69E-46 |
| MS.gene050577 | MS.gene067108 | 0.820351 | 7.18E-53 | -1.69E-46 |
| MS.gene050612 | MS.gene067108 | 0.811612 | 6.42E-51 | -1.69E-46 |
| MS.gene050747 | MS.gene067108 | 0.803637 | 3.17E-49 | -1.69E-46 |
| MS.gene05080 | MS.gene067108 | 0.817918 | 2.57E-52 | -1.69E-46 |
| MS.gene05081 | MS.gene067108 | 0.858924 | 5.90E-63 | -1.69E-46 |
| MS.gene050868 | MS.gene067108 | 0.805851 | 1.09E-49 | -1.69E-46 |
| MS.gene050871 | MS.gene067108 | 0.800829 | 1.20E-48 | -1.69E-46 |
| MS.gene051317 | MS.gene067108 | 0.813115 | 3.01E-51 | -1.69E-46 |
| MS.gene051439 | MS.gene067108 | -0.804498 | 2.10E-49 | -1.69E-46 |
| MS.gene051838 | MS.gene067108 | 0.858853 | 6.19E-63 | -1.69E-46 |
| MS.gene052129 | MS.gene067108 | -0.805423 | 1.34E-49 | -1.69E-46 |
| MS.gene052134 | MS.gene067108 | 0.802103 | 6.57E-49 | -1.69E-46 |
| MS.gene052501 | MS.gene067108 | 0.812283 | 4.58E-51 | -1.69E-46 |
| MS.gene052503 | MS.gene067108 | 0.8434 | 1.43E-58 | -1.69E-46 |
| MS.gene052505 | MS.gene067108 | 0.811204 | 7.87E-51 | -1.69E-46 |
| MS.gene052606 | MS.gene067108 | -0.800303 | 1.54E-48 | -1.69E-46 |
| MS.gene052823 | MS.gene067108 | 0.839246 | 1.78E-57 | -1.69E-46 |
| MS.gene052825 | MS.gene067108 | 0.836047 | 1.18E-56 | -1.69E-46 |
| MS.gene052870 | MS.gene067108 | 0.810856 | 9.36E-51 | -1.69E-46 |
| MS.gene052872 | MS.gene067108 | 0.820207 | 7.75E-53 | -1.69E-46 |
| MS.gene053203 | MS.gene067108 | 0.811686 | 6.18E-51 | -1.69E-46 |
| MS.gene053479 | MS.gene067108 | 0.806237 | 9.07E-50 | -1.69E-46 |
| MS.gene053480 | MS.gene067108 | 0.829971 | 3.82E-55 | -1.69E-46 |
| MS.gene053579 | MS.gene067108 | 0.800062 | 1.72E-48 | -1.69E-46 |
| MS.gene053824 | MS.gene067108 | 0.814158 | 1.78E-51 | -1.69E-46 |
| MS.gene053916 | MS.gene067108 | 0.800631 | 1.32E-48 | -1.69E-46 |
| MS.gene054343 | MS.gene067108 | 0.828941 | 6.79E-55 | -1.69E-46 |
| MS.gene054344 | MS.gene067108 | 0.81901 | 1.45E-52 | -1.69E-46 |
| MS.gene054600 | MS.gene067108 | 0.800346 | 1.50E-48 | -1.69E-46 |
| MS.gene054706 | MS.gene067108 | -0.805031 | 1.62E-49 | -1.69E-46 |
| MS.gene05470 | MS.gene067108 | 0.836104 | 1.14E-56 | -1.69E-46 |
| MS.gene054884 | MS.gene067108 | -0.85616 | 3.88E-62 | -1.69E-46 |
| MS.gene055206 | MS.gene067108 | 0.823635 | 1.25E-53 | -1.69E-46 |
| MS.gene055289 | MS.gene067108 | 0.825114 | 5.59E-54 | -1.69E-46 |
| MS.gene055370 | MS.gene067108 | 0.805334 | 1.40E-49 | -1.69E-46 |
| MS.gene055502 | MS.gene067108 | 0.823244 | 1.54E-53 | -1.69E-46 |
| MS.gene055731 | MS.gene067108 | -0.80498 | 1.66E-49 | -1.69E-46 |
| MS.gene055748 | MS.gene067108 | 0.841637 | 4.21E-58 | -1.69E-46 |
| MS.gene055750 | MS.gene067108 | 0.800591 | 1.34E-48 | -1.69E-46 |
| MS.gene055751 | MS.gene067108 | 0.830038 | 3.68E-55 | -1.69E-46 |
| MS.gene05589 | MS.gene067108 | 0.862051 | 6.66E-64 | -1.69E-46 |
| MS.gene056387 | MS.gene067108 | 0.815791 | 7.72E-52 | -1.69E-46 |
| MS.gene056504 | MS.gene067108 | 0.829706 | 4.43E-55 | -1.69E-46 |
| MS.gene056590 | MS.gene067108 | -0.834464 | 2.95E-56 | -1.69E-46 |
| MS.gene056591 | MS.gene067108 | -0.835006 | 2.16E-56 | -1.69E-46 |
| MS.gene056633 | MS.gene067108 | 0.800265 | 1.56E-48 | -1.69E-46 |
| MS.gene056692 | MS.gene067108 | 0.80602 | 1.01E-49 | -1.69E-46 |
| MS.gene056694 | MS.gene067108 | -0.835408 | 1.71E-56 | -1.69E-46 |
| MS.gene056697 | MS.gene067108 | -0.845217 | 4.66E-59 | -1.69E-46 |
| MS.gene056856 | MS.gene067108 | -0.803198 | 3.91E-49 | -1.69E-46 |
| MS.gene057332 | MS.gene067108 | 0.832506 | 9.09E-56 | -1.69E-46 |
| MS.gene057658 | MS.gene067108 | 0.849751 | 2.64E-60 | -1.69E-46 |
| MS.gene057831 | MS.gene067108 | 0.804661 | 1.94E-49 | -1.69E-46 |
| MS.gene058334 | MS.gene067108 | -0.82832 | 9.60E-55 | -1.69E-46 |
| MS.gene058338 | MS.gene067108 | 0.819207 | 1.31E-52 | -1.69E-46 |
| MS.gene058419 | MS.gene067108 | 0.827871 | 1.23E-54 | -1.69E-46 |
| MS.gene058544 | MS.gene067108 | 0.835133 | 2.00E-56 | -1.69E-46 |
| MS.gene058838 | MS.gene067108 | 0.871938 | 4.66E-67 | -1.69E-46 |
| MS.gene059023 | MS.gene067108 | 0.846391 | 2.24E-59 | -1.69E-46 |
| MS.gene05914 | MS.gene067108 | 0.857488 | 1.58E-62 | -1.69E-46 |
| MS.gene05926 | MS.gene067108 | -0.806623 | 7.52E-50 | -1.69E-46 |
| MS.gene05934 | MS.gene067108 | 0.806296 | 8.81E-50 | -1.69E-46 |
| MS.gene059512 | MS.gene067108 | 0.805085 | 1.58E-49 | -1.69E-46 |
| MS.gene05960 | MS.gene067108 | -0.809602 | 1.75E-50 | -1.69E-46 |
| MS.gene059667 | MS.gene067108 | 0.812762 | 3.60E-51 | -1.69E-46 |
| MS.gene059677 | MS.gene067108 | 0.815934 | 7.17E-52 | -1.69E-46 |
| MS.gene060087 | MS.gene067108 | -0.800029 | 1.75E-48 | -1.69E-46 |
| MS.gene060405 | MS.gene067108 | -0.80383 | 2.89E-49 | -1.69E-46 |
| MS.gene060762 | MS.gene067108 | 0.835126 | 2.01E-56 | -1.69E-46 |
| MS.gene06092 | MS.gene067108 | 0.807268 | 5.49E-50 | -1.69E-46 |
| MS.gene060982 | MS.gene067108 | 0.809983 | 1.44E-50 | -1.69E-46 |
| MS.gene061044 | MS.gene067108 | -0.833312 | 5.73E-56 | -1.69E-46 |
| MS.gene061150 | MS.gene067108 | 0.80552 | 1.28E-49 | -1.69E-46 |
| MS.gene061231 | MS.gene067108 | 0.833414 | 5.41E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067108.t1 | MTR_7g406840 | 98.762 | 323 | 4 | 0 | 2 | 324 | 12 | 334 | 0.0 | 654 |
| MS.gene067108.t1 | MTR_1g087750 | 51.393 | 323 | 154 | 2 | 3 | 322 | 58 | 380 | 3.25e-115 | 342 |
| MS.gene067108.t1 | MTR_1g087750 | 45.699 | 372 | 150 | 4 | 3 | 322 | 58 | 429 | 8.64e-109 | 327 |
| MS.gene067108.t1 | MTR_7g102840 | 48.466 | 326 | 163 | 4 | 3 | 324 | 75 | 399 | 1.28e-103 | 313 |
| MS.gene067108.t1 | MTR_1g062970 | 49.235 | 327 | 158 | 6 | 3 | 324 | 78 | 401 | 9.81e-102 | 306 |
| MS.gene067108.t1 | MTR_3g117990 | 44.377 | 329 | 172 | 4 | 3 | 324 | 33 | 357 | 9.85e-102 | 305 |
| MS.gene067108.t1 | MTR_7g102870 | 47.095 | 327 | 167 | 5 | 3 | 324 | 76 | 401 | 2.22e-101 | 307 |
| MS.gene067108.t1 | MTR_7g112720 | 47.289 | 332 | 165 | 4 | 1 | 324 | 42 | 371 | 2.38e-98 | 298 |
| MS.gene067108.t1 | MTR_3g117990 | 45.000 | 300 | 154 | 4 | 3 | 295 | 33 | 328 | 6.06e-93 | 281 |
| MS.gene067108.t1 | MTR_3g117990 | 46.520 | 273 | 135 | 4 | 3 | 268 | 33 | 301 | 3.53e-89 | 269 |
| MS.gene067108.t1 | MTR_7g112720 | 43.388 | 242 | 127 | 4 | 91 | 324 | 1 | 240 | 3.20e-64 | 206 |
| MS.gene067108.t1 | MTR_6g045583 | 49.412 | 85 | 20 | 2 | 52 | 130 | 22 | 89 | 4.44e-12 | 65.5 |
| MS.gene067108.t1 | MTR_5g049870 | 49.412 | 85 | 20 | 2 | 52 | 130 | 22 | 89 | 4.85e-12 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene067108.t1 | AT5G47900 | 63.804 | 326 | 114 | 1 | 3 | 324 | 59 | 384 | 7.64e-150 | 427 |
| MS.gene067108.t1 | AT5G47900 | 64.000 | 325 | 113 | 1 | 4 | 324 | 1 | 325 | 2.74e-149 | 425 |
| MS.gene067108.t1 | AT5G47900 | 63.804 | 326 | 114 | 1 | 3 | 324 | 59 | 384 | 9.21e-149 | 426 |
| MS.gene067108.t1 | AT5G47900 | 63.804 | 326 | 114 | 1 | 3 | 324 | 59 | 384 | 9.21e-149 | 426 |
| MS.gene067108.t1 | AT5G47900 | 57.300 | 363 | 114 | 2 | 3 | 324 | 59 | 421 | 5.26e-142 | 408 |
| MS.gene067108.t1 | AT5G47900 | 66.171 | 269 | 87 | 1 | 3 | 267 | 59 | 327 | 2.04e-128 | 371 |
| MS.gene067108.t1 | AT5G47900 | 66.171 | 269 | 87 | 1 | 3 | 267 | 53 | 321 | 2.73e-127 | 371 |
| MS.gene067108.t1 | AT5G47900 | 62.637 | 273 | 98 | 1 | 56 | 324 | 12 | 284 | 5.76e-118 | 343 |
| MS.gene067108.t1 | AT5G27730 | 48.930 | 327 | 162 | 3 | 1 | 322 | 48 | 374 | 5.66e-106 | 315 |
| MS.gene067108.t1 | AT5G27730 | 49.231 | 325 | 160 | 3 | 3 | 322 | 50 | 374 | 4.52e-105 | 315 |
| MS.gene067108.t1 | AT5G27730 | 50.178 | 281 | 136 | 2 | 1 | 277 | 48 | 328 | 3.32e-97 | 291 |
| MS.gene067108.t1 | AT5G47900 | 62.441 | 213 | 79 | 1 | 3 | 214 | 59 | 271 | 1.58e-90 | 272 |
| MS.gene067108.t1 | AT5G47900 | 62.000 | 200 | 72 | 1 | 129 | 324 | 1 | 200 | 3.89e-90 | 270 |
| MS.gene067108.t1 | AT5G47900 | 61.111 | 216 | 80 | 1 | 3 | 214 | 59 | 274 | 9.73e-90 | 270 |
| MS.gene067108.t1 | AT5G27730 | 45.263 | 285 | 151 | 3 | 43 | 322 | 9 | 293 | 1.58e-81 | 249 |
| MS.gene067108.t1 | AT5G27730 | 45.263 | 285 | 151 | 3 | 43 | 322 | 9 | 293 | 1.93e-80 | 249 |
Find 76 sgRNAs with CRISPR-Local
Find 141 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCATTGCCTTGGATTTATT+TGG | 0.173138 | 7.3:-91313047 | MS.gene067108:intron |
| TGCCTCCTGATGCTCCTTCT+TGG | 0.195149 | 7.3:-91313645 | MS.gene067108:CDS |
| TTACAGCTCTCTGTGAAATT+TGG | 0.205026 | 7.3:-91314533 | MS.gene067108:CDS |
| GCTGCTGGCATTCTCTTTGC+TGG | 0.289082 | 7.3:-91312889 | MS.gene067108:CDS |
| CGTGAAGATATCGTTAATTC+TGG | 0.293626 | 7.3:-91314504 | MS.gene067108:CDS |
| CGCGTTAGTGATTTGACGTT+TGG | 0.319191 | 7.3:-91314715 | MS.gene067108:CDS |
| ATTTATCTTTGCTTGCTGTA+TGG | 0.319433 | 7.3:-91314210 | MS.gene067108:CDS |
| CTCAATGCAGCTTATGATAT+TGG | 0.322014 | 7.3:-91315523 | None:intergenic |
| GCGCTTAAGCTTCTTGCATT+AGG | 0.338601 | 7.3:-91315145 | MS.gene067108:CDS |
| ACCTCTTGTCTTGTAGTATC+TGG | 0.341845 | 7.3:-91313068 | MS.gene067108:CDS |
| TGCTTGGTTGGCTTGCATTA+TGG | 0.344938 | 7.3:-91313363 | MS.gene067108:CDS |
| CTTTGCTGGAATATATTTGA+TGG | 0.349023 | 7.3:-91312875 | None:intergenic |
| TGTCTCACTGCTGGTGCTGC+TGG | 0.374086 | 7.3:-91312904 | MS.gene067108:CDS |
| TTTATCTTTGCTTGCTGTAT+GGG | 0.383070 | 7.3:-91314209 | MS.gene067108:CDS |
| TGTTGGGAATTACCTTGAAA+TGG | 0.386259 | 7.3:+91313328 | None:intergenic |
| GATATCGATACTTTCTCAAA+AGG | 0.392446 | 7.3:+91314475 | None:intergenic |
| AGTCACCTTAAGAGTCCTTC+AGG | 0.392641 | 7.3:+91313604 | None:intergenic |
| ACAGGCGCCCGTGCATATAT+TGG | 0.393241 | 7.3:+91313899 | None:intergenic |
| TTTGAAGCAGATAAGGTTGA+TGG | 0.407438 | 7.3:-91314686 | MS.gene067108:CDS |
| TTGATCCTGAAGGACTCTTA+AGG | 0.410225 | 7.3:-91313609 | MS.gene067108:intron |
| TGTAGTATCTGGCATTGCCT+TGG | 0.412434 | 7.3:-91313057 | MS.gene067108:CDS |
| ACACTCACAGAAAATTTCAT+AGG | 0.413861 | 7.3:+91314135 | None:intergenic |
| AGTATAAATTCTCCTGACTA+TGG | 0.416712 | 7.3:-91313673 | MS.gene067108:CDS |
| GCAATTGTTACCTGCTTGGT+TGG | 0.430215 | 7.3:-91313375 | MS.gene067108:CDS |
| GATCATTCCAACGACATTGC+AGG | 0.438809 | 7.3:+91313961 | None:intergenic |
| GAGTGGATTTGAAGCAGATA+AGG | 0.447061 | 7.3:-91314693 | MS.gene067108:CDS |
| ATGATCGATCGTAGCTTGTT+GGG | 0.449187 | 7.3:-91313944 | MS.gene067108:CDS |
| TGGGATGTACGTTCCCGATT+GGG | 0.453793 | 7.3:-91314190 | MS.gene067108:CDS |
| AGTTATACTTGTCTCACTGC+TGG | 0.456351 | 7.3:-91312913 | MS.gene067108:CDS |
| AATGATCGATCGTAGCTTGT+TGG | 0.458059 | 7.3:-91313945 | MS.gene067108:CDS |
| TGACACCAAGAAGGAGCATC+AGG | 0.462233 | 7.3:+91313640 | None:intergenic |
| GGAGGCAATGGTCCATAGTC+AGG | 0.466457 | 7.3:+91313661 | None:intergenic |
| AATTTCATAGGTTCTGAAGA+AGG | 0.469705 | 7.3:+91314147 | None:intergenic |
| GTCGCATCAACTTTGCAAGA+TGG | 0.475648 | 7.3:+91315187 | None:intergenic |
| TGAGAAAGTATCGATATCAA+TGG | 0.477114 | 7.3:-91314470 | MS.gene067108:intron |
| TCATATTATTCTCCATTTCA+AGG | 0.480946 | 7.3:-91313340 | MS.gene067108:intron |
| GATGGCAATTGTTACCTGCT+TGG | 0.491107 | 7.3:-91313379 | MS.gene067108:CDS |
| ATGATATTGGTGGATGATGC+TGG | 0.492588 | 7.3:-91315510 | MS.gene067108:CDS |
| ATATCGATACTTTCTCAAAA+GGG | 0.495414 | 7.3:+91314476 | None:intergenic |
| AAGAGTCCTTCAGGATCAAA+AGG | 0.496523 | 7.3:+91313613 | None:intergenic |
| ATGGGATGTACGTTCCCGAT+TGG | 0.499827 | 7.3:-91314191 | MS.gene067108:CDS |
| GGTTCTGAAGAAGGTTCTGT+TGG | 0.504371 | 7.3:+91314156 | None:intergenic |
| TGGAACTTCATACTCCCAAT+CGG | 0.507436 | 7.3:+91314176 | None:intergenic |
| TGGGTTTACAACATCTATAC+CGG | 0.509722 | 7.3:-91313925 | MS.gene067108:CDS |
| CAAAGTTGATGCGACTAGAA+AGG | 0.510229 | 7.3:-91315179 | MS.gene067108:CDS |
| GCTATTGTTAAACCATTCCA+TGG | 0.518565 | 7.3:+91315459 | None:intergenic |
| CTCAATCATTCTCCATGGAA+TGG | 0.518820 | 7.3:-91315471 | MS.gene067108:CDS |
| TGCACTAGCATTCACATACA+AGG | 0.532824 | 7.3:-91315397 | MS.gene067108:intron |
| CATGGAGAATGATTGAGTGC+TGG | 0.551745 | 7.3:+91315477 | None:intergenic |
| AAGGTTGATGGGAATACTGC+AGG | 0.554486 | 7.3:-91314674 | MS.gene067108:intron |
| GGACCAGCCTGCAATGTCGT+TGG | 0.555369 | 7.3:-91313968 | MS.gene067108:CDS |
| TTGAAGCAGATAAGGTTGAT+GGG | 0.558495 | 7.3:-91314685 | MS.gene067108:CDS |
| TAGTGATTTGACGTTTGGAG+TGG | 0.558670 | 7.3:-91314710 | MS.gene067108:CDS |
| TGTGGAGTAAGAGGCGACAC+TGG | 0.561390 | 7.3:-91313989 | MS.gene067108:CDS |
| TAGGTTAAATGTGGAGTAAG+AGG | 0.567721 | 7.3:-91313998 | MS.gene067108:CDS |
| GAAGGAGCATCAGGAGGCAA+TGG | 0.569145 | 7.3:+91313649 | None:intergenic |
| GGAACTTCATACTCCCAATC+GGG | 0.571048 | 7.3:+91314177 | None:intergenic |
| ATGGAATTATAGTTCAGTGA+TGG | 0.571322 | 7.3:-91313397 | MS.gene067108:intron |
| AATGTAAGATTCTAATTCTG+TGG | 0.580044 | 7.3:+91313102 | None:intergenic |
| ATGGTTACCAAATAAATCCA+AGG | 0.585044 | 7.3:+91313040 | None:intergenic |
| CAGCACTCAATCATTCTCCA+TGG | 0.586892 | 7.3:-91315476 | MS.gene067108:CDS |
| CCCGTGCATATATTGGTCTC+CGG | 0.589463 | 7.3:+91313906 | None:intergenic |
| ACAGAATTAGAATCTTACAT+TGG | 0.594630 | 7.3:-91313100 | MS.gene067108:CDS |
| AATGCAGCTTATGATATTGG+TGG | 0.594795 | 7.3:-91315520 | MS.gene067108:CDS |
| ATTCCAACGACATTGCAGGC+TGG | 0.596770 | 7.3:+91313965 | None:intergenic |
| ATAATGCAAGCCAACCAAGC+AGG | 0.596844 | 7.3:+91313365 | None:intergenic |
| GCCAGATACTACAAGACAAG+AGG | 0.600740 | 7.3:+91313067 | None:intergenic |
| GATACTACAAGACAAGAGGT+TGG | 0.600915 | 7.3:+91313071 | None:intergenic |
| AAAGGAGCTTGACACCAAGA+AGG | 0.617752 | 7.3:+91313631 | None:intergenic |
| ACCGGAGACCAATATATGCA+CGG | 0.621670 | 7.3:-91313907 | MS.gene067108:CDS |
| CCGGAGACCAATATATGCAC+GGG | 0.629013 | 7.3:-91313906 | MS.gene067108:CDS |
| AACATGTGTTAGGTTAAATG+TGG | 0.637640 | 7.3:-91314007 | MS.gene067108:intron |
| TCATTGGATGCAGAGAATTG+CGG | 0.648931 | 7.3:-91314568 | MS.gene067108:intron |
| CACCAAGAAGGAGCATCAGG+AGG | 0.654110 | 7.3:+91313643 | None:intergenic |
| AGTTGATGCGACTAGAAAGG+CGG | 0.689224 | 7.3:-91315176 | MS.gene067108:CDS |
| ATATTGGTGGATGATGCTGG+TGG | 0.716550 | 7.3:-91315507 | MS.gene067108:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTTTTTGAATATAACA+GGG | - | chr7.3:91314139-91314158 | MS.gene067108:intron | 10.0% |
| !!! | TATTTTTTTTTGAATATAAC+AGG | - | chr7.3:91314138-91314157 | MS.gene067108:intron | 10.0% |
| !! | ATTATAACTGTAAATTCAAA+AGG | + | chr7.3:91314655-91314674 | None:intergenic | 15.0% |
| !! | TAATAAAAAACCAAAATGTT+GGG | + | chr7.3:91315086-91315105 | None:intergenic | 15.0% |
| !! | TTTGAATTTACAGTTATAAT+TGG | - | chr7.3:91314655-91314674 | MS.gene067108:intron | 15.0% |
| !!! | CAATTTAACTTCTTATATAT+TGG | - | chr7.3:91314337-91314356 | MS.gene067108:intron | 15.0% |
| !!! | GAATTTTAGTTTCTTAATAT+AGG | - | chr7.3:91313967-91313986 | MS.gene067108:CDS | 15.0% |
| !!! | TCATTCTTTATTTATTTCAT+TGG | - | chr7.3:91313811-91313830 | MS.gene067108:intron | 15.0% |
| !! | AATTTATATAGCATGGTTTA+TGG | - | chr7.3:91314938-91314957 | MS.gene067108:intron | 20.0% |
| !! | ATATAGATCAAAGAATTCAA+AGG | + | chr7.3:91313090-91313109 | None:intergenic | 20.0% |
| !! | ATGAATTTAAACTTCCAAAT+AGG | + | chr7.3:91314308-91314327 | None:intergenic | 20.0% |
| !! | CCAACAATAAAAAGAAAAAA+CGG | + | chr7.3:91312975-91312994 | None:intergenic | 20.0% |
| !! | GTAATAAAAAACCAAAATGT+TGG | + | chr7.3:91315087-91315106 | None:intergenic | 20.0% |
| !! | TATCACAAAATTTAAGCAAA+AGG | + | chr7.3:91313302-91313321 | None:intergenic | 20.0% |
| !! | TCATTGAAATTTATATAGCA+TGG | - | chr7.3:91314931-91314950 | MS.gene067108:intron | 20.0% |
| !!! | GGTTTTTTATTACTTTCTTT+AGG | - | chr7.3:91315094-91315113 | MS.gene067108:intron | 20.0% |
| ! | AAGAAAGAGTTAAGAGATAA+TGG | + | chr7.3:91315377-91315396 | None:intergenic | 25.0% |
| ! | AATGCAATACATTACATACA+TGG | + | chr7.3:91313633-91313652 | None:intergenic | 25.0% |
| ! | AATGTAAGATTCTAATTCTG+TGG | + | chr7.3:91315296-91315315 | None:intergenic | 25.0% |
| ! | ACAGAATTAGAATCTTACAT+TGG | - | chr7.3:91315295-91315314 | MS.gene067108:intron | 25.0% |
| ! | AGACTTTCAATATAAAGTGT+TGG | - | chr7.3:91315208-91315227 | MS.gene067108:intron | 25.0% |
| ! | ATATCGATACTTTCTCAAAA+GGG | + | chr7.3:91313922-91313941 | None:intergenic | 25.0% |
| ! | CAAAATTTAAGCAAAAGGTT+CGG | + | chr7.3:91313297-91313316 | None:intergenic | 25.0% |
| ! | TCATATTATTCTCCATTTCA+AGG | - | chr7.3:91315055-91315074 | MS.gene067108:intron | 25.0% |
| !! | AAGGTGTTAAAGTAAAATGT+TGG | - | chr7.3:91313378-91313397 | MS.gene067108:CDS | 25.0% |
| !! | CTCTAATTTTGTGTCTATTA+TGG | - | chr7.3:91314979-91314998 | MS.gene067108:intron | 25.0% |
| !! | GAATTGCATTCTAAAAACAA+AGG | + | chr7.3:91314831-91314850 | None:intergenic | 25.0% |
| !! | GTGTAACTTTTTATTACAAC+AGG | - | chr7.3:91314694-91314713 | MS.gene067108:CDS | 25.0% |
| !! | TTAAAGTAAAATGTTGGTGA+TGG | - | chr7.3:91313384-91313403 | MS.gene067108:CDS | 25.0% |
| !! | TTTACATTTTCACACCTATT+TGG | - | chr7.3:91314291-91314310 | MS.gene067108:intron | 25.0% |
| !! | TTTTTAATAATCAGACGACT+AGG | + | chr7.3:91314611-91314630 | None:intergenic | 25.0% |
| !!! | CCGTTTTTTCTTTTTATTGT+TGG | - | chr7.3:91312972-91312991 | MS.gene067108:intron | 25.0% |
| !!! | TCTTTTTTTGATGAATGTCA+AGG | - | chr7.3:91313359-91313378 | MS.gene067108:CDS | 25.0% |
| !!! | TGTTATTTCACAACATATAC+AGG | + | chr7.3:91313427-91313446 | None:intergenic | 25.0% |
| AAAAGCAACATCACATAACT+AGG | + | chr7.3:91314070-91314089 | None:intergenic | 30.0% | |
| AACATGTGTTAGGTTAAATG+TGG | - | chr7.3:91314388-91314407 | MS.gene067108:intron | 30.0% | |
| AATTGGTTGTAAGTTGTTGA+CGG | - | chr7.3:91314672-91314691 | MS.gene067108:intron | 30.0% | |
| ACACTCACAGAAAATTTCAT+AGG | + | chr7.3:91314263-91314282 | None:intergenic | 30.0% | |
| AGTATAAATTCTCCTGACTA+TGG | - | chr7.3:91314722-91314741 | MS.gene067108:CDS | 30.0% | |
| ATAGTGTATCACAAAACAAC+AGG | + | chr7.3:91313494-91313513 | None:intergenic | 30.0% | |
| ATGGAATTATAGTTCAGTGA+TGG | - | chr7.3:91314998-91315017 | MS.gene067108:intron | 30.0% | |
| ATGGTTACCAAATAAATCCA+AGG | + | chr7.3:91315358-91315377 | None:intergenic | 30.0% | |
| GATATCGATACTTTCTCAAA+AGG | + | chr7.3:91313923-91313942 | None:intergenic | 30.0% | |
| TAGTGTATCACAAAACAACA+GGG | + | chr7.3:91313493-91313512 | None:intergenic | 30.0% | |
| TATCTATCTGAACATGTGTT+AGG | - | chr7.3:91314378-91314397 | MS.gene067108:intron | 30.0% | |
| TTCCTTATATTGCTAAATGC+AGG | - | chr7.3:91315269-91315288 | MS.gene067108:intron | 30.0% | |
| TTCTCGATATGAAGGAAAAT+TGG | - | chr7.3:91314848-91314867 | MS.gene067108:intron | 30.0% | |
| TTCTGTTCAAGAAACAGATA+AGG | + | chr7.3:91313187-91313206 | None:intergenic | 30.0% | |
| TTGTGAAATAACAGCTATAG+CGG | - | chr7.3:91313434-91313453 | MS.gene067108:intron | 30.0% | |
| ! | AACGAAGTTCTAACTTCATA+GGG | + | chr7.3:91313759-91313778 | None:intergenic | 30.0% |
| ! | ATAAGCTCTTGTTGTAATGT+AGG | - | chr7.3:91315428-91315447 | MS.gene067108:CDS | 30.0% |
| ! | TTTTGAATATAACAGGGCTT+TGG | - | chr7.3:91314145-91314164 | MS.gene067108:CDS | 30.0% |
| !! | AATTTCATAGGTTCTGAAGA+AGG | + | chr7.3:91314251-91314270 | None:intergenic | 30.0% |
| !! | ATTTATCTTTGCTTGCTGTA+TGG | - | chr7.3:91314185-91314204 | MS.gene067108:CDS | 30.0% |
| !! | TGAGAAAGTATCGATATCAA+TGG | - | chr7.3:91313925-91313944 | MS.gene067108:CDS | 30.0% |
| !! | TTTATCTTTGCTTGCTGTAT+GGG | - | chr7.3:91314186-91314205 | MS.gene067108:CDS | 30.0% |
| CGATCTACGATTGACAAAAT+TGG | - | chr7.3:91313599-91313618 | MS.gene067108:intron | 35.0% | |
| CGTGAAGATATCGTTAATTC+TGG | - | chr7.3:91313891-91313910 | MS.gene067108:intron | 35.0% | |
| GAATGCAATTCTCGATATGA+AGG | - | chr7.3:91314840-91314859 | MS.gene067108:intron | 35.0% | |
| GACTTGAGACACAAGTTAAA+GGG | + | chr7.3:91314093-91314112 | None:intergenic | 35.0% | |
| GCTATTGTTAAACCATTCCA+TGG | + | chr7.3:91312939-91312958 | None:intergenic | 35.0% | |
| GTCCTGCATTTAGCAATATA+AGG | + | chr7.3:91315274-91315293 | None:intergenic | 35.0% | |
| TAGGTTAAATGTGGAGTAAG+AGG | - | chr7.3:91314397-91314416 | MS.gene067108:intron | 35.0% | |
| TGACTTGAGACACAAGTTAA+AGG | + | chr7.3:91314094-91314113 | None:intergenic | 35.0% | |
| TGGGTTTACAACATCTATAC+CGG | - | chr7.3:91314470-91314489 | MS.gene067108:intron | 35.0% | |
| TGTAATCACATGTATCGTGT+CGG | - | chr7.3:91315170-91315189 | MS.gene067108:CDS | 35.0% | |
| TGTTGGGAATTACCTTGAAA+TGG | + | chr7.3:91315070-91315089 | None:intergenic | 35.0% | |
| TTACAGCTCTCTGTGAAATT+TGG | - | chr7.3:91313862-91313881 | MS.gene067108:intron | 35.0% | |
| ! | AAGAGTATAGAGGATCATAC+AGG | + | chr7.3:91314517-91314536 | None:intergenic | 35.0% |
| ! | ATTTAAGCAAAAGGTTCGGT+TGG | + | chr7.3:91313293-91313312 | None:intergenic | 35.0% |
| ! | CAACGAAGTTCTAACTTCAT+AGG | + | chr7.3:91313760-91313779 | None:intergenic | 35.0% |
| ! | CAAGGTAATTCCCAACATTT+TGG | - | chr7.3:91315073-91315092 | MS.gene067108:intron | 35.0% |
| ! | CAGAACAACTAAGAGTATAG+AGG | + | chr7.3:91314527-91314546 | None:intergenic | 35.0% |
| ! | GCATTAGGCATTTTTCTTCA+AGG | - | chr7.3:91313265-91313284 | MS.gene067108:intron | 35.0% |
| ! | TTGAAGCAGATAAGGTTGAT+GGG | - | chr7.3:91313710-91313729 | MS.gene067108:intron | 35.0% |
| ! | TTGACGGTTTGTTCAAATTC+CGG | + | chr7.3:91313573-91313592 | None:intergenic | 35.0% |
| ! | TTTGAAGCAGATAAGGTTGA+TGG | - | chr7.3:91313709-91313728 | MS.gene067108:intron | 35.0% |
| !! | TATTGCATTTTGTCGTTTGC+AGG | - | chr7.3:91313644-91313663 | MS.gene067108:CDS | 35.0% |
| !! | TTAACTTGTGTCTCAAGTCA+TGG | - | chr7.3:91314094-91314113 | MS.gene067108:intron | 35.0% |
| AAGAGTCCTTCAGGATCAAA+AGG | + | chr7.3:91314785-91314804 | None:intergenic | 40.0% | |
| ACCTCTTGTCTTGTAGTATC+TGG | - | chr7.3:91315327-91315346 | MS.gene067108:intron | 40.0% | |
| AGTTATACTTGTCTCACTGC+TGG | - | chr7.3:91315482-91315501 | MS.gene067108:CDS | 40.0% | |
| CTAGCATTCACATACAAGGT+TGG | - | chr7.3:91313002-91313021 | MS.gene067108:intron | 40.0% | |
| CTCAATCATTCTCCATGGAA+TGG | - | chr7.3:91312924-91312943 | MS.gene067108:CDS | 40.0% | |
| GATACTACAAGACAAGAGGT+TGG | + | chr7.3:91315327-91315346 | None:intergenic | 40.0% | |
| GGCATTGCCTTGGATTTATT+TGG | - | chr7.3:91315348-91315367 | MS.gene067108:intron | 40.0% | |
| GTAGGTGTTAATGTCGTGTT+TGG | + | chr7.3:91315131-91315150 | None:intergenic | 40.0% | |
| TAGTGATTTGACGTTTGGAG+TGG | - | chr7.3:91313685-91313704 | MS.gene067108:intron | 40.0% | |
| TCATTGGATGCAGAGAATTG+CGG | - | chr7.3:91313827-91313846 | MS.gene067108:intron | 40.0% | |
| TCGTAGATCGCGTAAATTGA+CGG | + | chr7.3:91313589-91313608 | None:intergenic | 40.0% | |
| TGCACTAGCATTCACATACA+AGG | - | chr7.3:91312998-91313017 | MS.gene067108:intron | 40.0% | |
| TGGAACTTCATACTCCCAAT+CGG | + | chr7.3:91314222-91314241 | None:intergenic | 40.0% | |
| TTGATCCTGAAGGACTCTTA+AGG | - | chr7.3:91314786-91314805 | MS.gene067108:intron | 40.0% | |
| ! | AATGATCGATCGTAGCTTGT+TGG | - | chr7.3:91314450-91314469 | MS.gene067108:intron | 40.0% |
| ! | ATGATATTGGTGGATGATGC+TGG | - | chr7.3:91312885-91312904 | MS.gene067108:CDS | 40.0% |
| ! | GAGTGGATTTGAAGCAGATA+AGG | - | chr7.3:91313702-91313721 | MS.gene067108:intron | 40.0% |
| ! | GATTACATTGAAAGCGTGTC+TGG | + | chr7.3:91315157-91315176 | None:intergenic | 40.0% |
| !! | ATGATCGATCGTAGCTTGTT+GGG | - | chr7.3:91314451-91314470 | MS.gene067108:intron | 40.0% |
| !! | CAAAGTTGATGCGACTAGAA+AGG | - | chr7.3:91313216-91313235 | MS.gene067108:intron | 40.0% |
| AAAGGAGCTTGACACCAAGA+AGG | + | chr7.3:91314767-91314786 | None:intergenic | 45.0% | |
| ACCGGAGACCAATATATGCA+CGG | - | chr7.3:91314488-91314507 | MS.gene067108:CDS | 45.0% | |
| AGTCACCTTAAGAGTCCTTC+AGG | + | chr7.3:91314794-91314813 | None:intergenic | 45.0% | |
| ATAATGCAAGCCAACCAAGC+AGG | + | chr7.3:91315033-91315052 | None:intergenic | 45.0% | |
| CAGCACTCAATCATTCTCCA+TGG | - | chr7.3:91312919-91312938 | MS.gene067108:CDS | 45.0% | |
| CATGGAGAATGATTGAGTGC+TGG | + | chr7.3:91312921-91312940 | None:intergenic | 45.0% | |
| CGCGTTAGTGATTTGACGTT+TGG | - | chr7.3:91313680-91313699 | MS.gene067108:CDS | 45.0% | |
| GATCATTCCAACGACATTGC+AGG | + | chr7.3:91314437-91314456 | None:intergenic | 45.0% | |
| GATGGCAATTGTTACCTGCT+TGG | - | chr7.3:91315016-91315035 | MS.gene067108:intron | 45.0% | |
| GCAATTGTTACCTGCTTGGT+TGG | - | chr7.3:91315020-91315039 | MS.gene067108:intron | 45.0% | |
| GCCAGATACTACAAGACAAG+AGG | + | chr7.3:91315331-91315350 | None:intergenic | 45.0% | |
| GCGCTTAAGCTTCTTGCATT+AGG | - | chr7.3:91313250-91313269 | MS.gene067108:intron | 45.0% | |
| GCTATAGCAGTGCAACATAC+CGG | - | chr7.3:91313551-91313570 | MS.gene067108:intron | 45.0% | |
| GGAACTTCATACTCCCAATC+GGG | + | chr7.3:91314221-91314240 | None:intergenic | 45.0% | |
| GTCGCATCAACTTTGCAAGA+TGG | + | chr7.3:91313211-91313230 | None:intergenic | 45.0% | |
| TGTAGTATCTGGCATTGCCT+TGG | - | chr7.3:91315338-91315357 | MS.gene067108:intron | 45.0% | |
| ! | AGTTGATGCGACTAGAAAGG+CGG | - | chr7.3:91313219-91313238 | MS.gene067108:intron | 45.0% |
| ! | ATATTGGTGGATGATGCTGG+TGG | - | chr7.3:91312888-91312907 | MS.gene067108:CDS | 45.0% |
| ! | CAAGCTCCTTTTGATCCTGA+AGG | - | chr7.3:91314776-91314795 | MS.gene067108:intron | 45.0% |
| ! | CGACTAGAAAGGCGGTTTTA+CGG | - | chr7.3:91313227-91313246 | MS.gene067108:intron | 45.0% |
| ! | GACTAGAAAGGCGGTTTTAC+GGG | - | chr7.3:91313228-91313247 | MS.gene067108:intron | 45.0% |
| !! | AAGGTTGATGGGAATACTGC+AGG | - | chr7.3:91313721-91313740 | MS.gene067108:intron | 45.0% |
| !! | GGTTCTGAAGAAGGTTCTGT+TGG | + | chr7.3:91314242-91314261 | None:intergenic | 45.0% |
| !! | TGCATTTTGTCGTTTGCAGG+AGG | - | chr7.3:91313647-91313666 | MS.gene067108:CDS | 45.0% |
| !! | TGCTTGGTTGGCTTGCATTA+TGG | - | chr7.3:91315032-91315051 | MS.gene067108:intron | 45.0% |
| ATGGGATGTACGTTCCCGAT+TGG | - | chr7.3:91314204-91314223 | MS.gene067108:CDS | 50.0% | |
| ATTCCAACGACATTGCAGGC+TGG | + | chr7.3:91314433-91314452 | None:intergenic | 50.0% | |
| CCGGAGACCAATATATGCAC+GGG | - | chr7.3:91314489-91314508 | MS.gene067108:CDS | 50.0% | |
| CGGCGCTATAACGTGCATAT+CGG | - | chr7.3:91313454-91313473 | MS.gene067108:intron | 50.0% | |
| TGGGATGTACGTTCCCGATT+GGG | - | chr7.3:91314205-91314224 | MS.gene067108:CDS | 50.0% | |
| ! | CCCGTGCATATATTGGTCTC+CGG | + | chr7.3:91314492-91314511 | None:intergenic | 50.0% |
| ! | TGAAAGCGTGTCTGGTGTGT+AGG | + | chr7.3:91315149-91315168 | None:intergenic | 50.0% |
| !! | TGACACCAAGAAGGAGCATC+AGG | + | chr7.3:91314758-91314777 | None:intergenic | 50.0% |
| ACAGGCGCCCGTGCATATAT+TGG | + | chr7.3:91314499-91314518 | None:intergenic | 55.0% | |
| GCTGCTGGCATTCTCTTTGC+TGG | - | chr7.3:91315506-91315525 | MS.gene067108:CDS | 55.0% | |
| GGAGGCAATGGTCCATAGTC+AGG | + | chr7.3:91314737-91314756 | None:intergenic | 55.0% | |
| TGCCTCCTGATGCTCCTTCT+TGG | - | chr7.3:91314750-91314769 | MS.gene067108:intron | 55.0% | |
| TGTGGAGTAAGAGGCGACAC+TGG | - | chr7.3:91314406-91314425 | MS.gene067108:intron | 55.0% | |
| !! | CACCAAGAAGGAGCATCAGG+AGG | + | chr7.3:91314755-91314774 | None:intergenic | 55.0% |
| !! | GAAGGAGCATCAGGAGGCAA+TGG | + | chr7.3:91314749-91314768 | None:intergenic | 55.0% |
| GGACCAGCCTGCAATGTCGT+TGG | - | chr7.3:91314427-91314446 | MS.gene067108:intron | 60.0% | |
| !! | TGTCTCACTGCTGGTGCTGC+TGG | - | chr7.3:91315491-91315510 | MS.gene067108:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 91312876 | 91315541 | 91312876 | ID=MS.gene067108 |
| chr7.3 | mRNA | 91312876 | 91315541 | 91312876 | ID=MS.gene067108.t1;Parent=MS.gene067108 |
| chr7.3 | exon | 91315398 | 91315541 | 91315398 | ID=MS.gene067108.t1.exon1;Parent=MS.gene067108.t1 |
| chr7.3 | CDS | 91315398 | 91315541 | 91315398 | ID=cds.MS.gene067108.t1;Parent=MS.gene067108.t1 |
| chr7.3 | exon | 91315131 | 91315212 | 91315131 | ID=MS.gene067108.t1.exon2;Parent=MS.gene067108.t1 |
| chr7.3 | CDS | 91315131 | 91315212 | 91315131 | ID=cds.MS.gene067108.t1;Parent=MS.gene067108.t1 |
| chr7.3 | exon | 91314675 | 91314751 | 91314675 | ID=MS.gene067108.t1.exon3;Parent=MS.gene067108.t1 |
| chr7.3 | CDS | 91314675 | 91314751 | 91314675 | ID=cds.MS.gene067108.t1;Parent=MS.gene067108.t1 |
| chr7.3 | exon | 91314471 | 91314577 | 91314471 | ID=MS.gene067108.t1.exon4;Parent=MS.gene067108.t1 |
| chr7.3 | CDS | 91314471 | 91314577 | 91314471 | ID=cds.MS.gene067108.t1;Parent=MS.gene067108.t1 |
| chr7.3 | exon | 91314143 | 91314257 | 91314143 | ID=MS.gene067108.t1.exon5;Parent=MS.gene067108.t1 |
| chr7.3 | CDS | 91314143 | 91314257 | 91314143 | ID=cds.MS.gene067108.t1;Parent=MS.gene067108.t1 |
| chr7.3 | exon | 91313901 | 91314017 | 91313901 | ID=MS.gene067108.t1.exon6;Parent=MS.gene067108.t1 |
| chr7.3 | CDS | 91313901 | 91314017 | 91313901 | ID=cds.MS.gene067108.t1;Parent=MS.gene067108.t1 |
| chr7.3 | exon | 91313610 | 91313701 | 91313610 | ID=MS.gene067108.t1.exon7;Parent=MS.gene067108.t1 |
| chr7.3 | CDS | 91313610 | 91313701 | 91313610 | ID=cds.MS.gene067108.t1;Parent=MS.gene067108.t1 |
| chr7.3 | exon | 91313341 | 91313407 | 91313341 | ID=MS.gene067108.t1.exon8;Parent=MS.gene067108.t1 |
| chr7.3 | CDS | 91313341 | 91313407 | 91313341 | ID=cds.MS.gene067108.t1;Parent=MS.gene067108.t1 |
| chr7.3 | exon | 91313048 | 91313126 | 91313048 | ID=MS.gene067108.t1.exon9;Parent=MS.gene067108.t1 |
| chr7.3 | CDS | 91313048 | 91313126 | 91313048 | ID=cds.MS.gene067108.t1;Parent=MS.gene067108.t1 |
| chr7.3 | exon | 91312876 | 91312967 | 91312876 | ID=MS.gene067108.t1.exon10;Parent=MS.gene067108.t1 |
| chr7.3 | CDS | 91312876 | 91312967 | 91312876 | ID=cds.MS.gene067108.t1;Parent=MS.gene067108.t1 |
| Gene Sequence |
| Protein sequence |
