Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068022.t1 | XP_003608396.1 | 98.8 | 85 | 1 | 0 | 1 | 85 | 1 | 85 | 6.90E-39 | 169.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068022.t1 | A2XKU9 | 81.2 | 85 | 16 | 0 | 1 | 85 | 1 | 85 | 3.2e-32 | 138.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068022.t1 | G7JV45 | 98.8 | 85 | 1 | 0 | 1 | 85 | 1 | 85 | 5.0e-39 | 169.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene068022 | 0.820256 | 7.55E-53 | -1.69E-46 |
| MS.gene049341 | MS.gene068022 | 0.814615 | 1.41E-51 | -1.69E-46 |
| MS.gene049350 | MS.gene068022 | 0.847309 | 1.25E-59 | -1.69E-46 |
| MS.gene049711 | MS.gene068022 | 0.812071 | 5.10E-51 | -1.69E-46 |
| MS.gene049807 | MS.gene068022 | 0.843888 | 1.06E-58 | -1.69E-46 |
| MS.gene049821 | MS.gene068022 | 0.818576 | 1.82E-52 | -1.69E-46 |
| MS.gene049850 | MS.gene068022 | 0.803826 | 2.90E-49 | -1.69E-46 |
| MS.gene049887 | MS.gene068022 | 0.863444 | 2.48E-64 | -1.69E-46 |
| MS.gene050020 | MS.gene068022 | 0.803165 | 3.97E-49 | -1.69E-46 |
| MS.gene050689 | MS.gene068022 | 0.819238 | 1.29E-52 | -1.69E-46 |
| MS.gene050807 | MS.gene068022 | 0.857726 | 1.34E-62 | -1.69E-46 |
| MS.gene050808 | MS.gene068022 | 0.821012 | 5.06E-53 | -1.69E-46 |
| MS.gene050838 | MS.gene068022 | 0.853484 | 2.32E-61 | -1.69E-46 |
| MS.gene050901 | MS.gene068022 | 0.851237 | 1.01E-60 | -1.69E-46 |
| MS.gene051029 | MS.gene068022 | 0.812956 | 3.27E-51 | -1.69E-46 |
| MS.gene051102 | MS.gene068022 | 0.820336 | 7.24E-53 | -1.69E-46 |
| MS.gene051234 | MS.gene068022 | 0.822693 | 2.07E-53 | -1.69E-46 |
| MS.gene051247 | MS.gene068022 | 0.80576 | 1.14E-49 | -1.69E-46 |
| MS.gene051328 | MS.gene068022 | 0.832178 | 1.10E-55 | -1.69E-46 |
| MS.gene05157 | MS.gene068022 | 0.848222 | 7.03E-60 | -1.69E-46 |
| MS.gene051700 | MS.gene068022 | 0.801194 | 1.01E-48 | -1.69E-46 |
| MS.gene051719 | MS.gene068022 | 0.864326 | 1.32E-64 | -1.69E-46 |
| MS.gene051720 | MS.gene068022 | 0.863307 | 2.73E-64 | -1.69E-46 |
| MS.gene051885 | MS.gene068022 | 0.830003 | 3.75E-55 | -1.69E-46 |
| MS.gene052133 | MS.gene068022 | 0.870316 | 1.60E-66 | -1.69E-46 |
| MS.gene052307 | MS.gene068022 | 0.805955 | 1.04E-49 | -1.69E-46 |
| MS.gene052335 | MS.gene068022 | 0.865578 | 5.33E-65 | -1.69E-46 |
| MS.gene052516 | MS.gene068022 | 0.839414 | 1.61E-57 | -1.69E-46 |
| MS.gene052609 | MS.gene068022 | 0.810435 | 1.15E-50 | -1.69E-46 |
| MS.gene052665 | MS.gene068022 | 0.833231 | 6.01E-56 | -1.69E-46 |
| MS.gene052732 | MS.gene068022 | 0.865094 | 7.57E-65 | -1.69E-46 |
| MS.gene052879 | MS.gene068022 | 0.815293 | 9.96E-52 | -1.69E-46 |
| MS.gene052947 | MS.gene068022 | 0.827649 | 1.39E-54 | -1.69E-46 |
| MS.gene053143 | MS.gene068022 | 0.841898 | 3.59E-58 | -1.69E-46 |
| MS.gene053144 | MS.gene068022 | 0.882725 | 8.19E-71 | -1.69E-46 |
| MS.gene053171 | MS.gene068022 | 0.85277 | 3.71E-61 | -1.69E-46 |
| MS.gene053174 | MS.gene068022 | 0.83303 | 6.74E-56 | -1.69E-46 |
| MS.gene053563 | MS.gene068022 | 0.802389 | 5.74E-49 | -1.69E-46 |
| MS.gene053581 | MS.gene068022 | 0.990723 | 5.22E-184 | -1.69E-46 |
| MS.gene053599 | MS.gene068022 | 0.814578 | 1.43E-51 | -1.69E-46 |
| MS.gene053678 | MS.gene068022 | 0.812259 | 4.64E-51 | -1.69E-46 |
| MS.gene054250 | MS.gene068022 | 0.806697 | 7.25E-50 | -1.69E-46 |
| MS.gene054550 | MS.gene068022 | 0.822701 | 2.06E-53 | -1.69E-46 |
| MS.gene054554 | MS.gene068022 | 0.84217 | 3.05E-58 | -1.69E-46 |
| MS.gene054584 | MS.gene068022 | 0.876709 | 1.13E-68 | -1.69E-46 |
| MS.gene05462 | MS.gene068022 | 0.800055 | 1.72E-48 | -1.69E-46 |
| MS.gene055144 | MS.gene068022 | 0.831432 | 1.67E-55 | -1.69E-46 |
| MS.gene055465 | MS.gene068022 | 0.861258 | 1.16E-63 | -1.69E-46 |
| MS.gene055466 | MS.gene068022 | 0.871237 | 7.96E-67 | -1.69E-46 |
| MS.gene055467 | MS.gene068022 | 0.812815 | 3.51E-51 | -1.69E-46 |
| MS.gene055468 | MS.gene068022 | 0.872525 | 2.98E-67 | -1.69E-46 |
| MS.gene055469 | MS.gene068022 | 0.847927 | 8.48E-60 | -1.69E-46 |
| MS.gene055513 | MS.gene068022 | 0.810018 | 1.42E-50 | -1.69E-46 |
| MS.gene055537 | MS.gene068022 | 0.850298 | 1.86E-60 | -1.69E-46 |
| MS.gene055580 | MS.gene068022 | -0.855204 | 7.38E-62 | -1.69E-46 |
| MS.gene055764 | MS.gene068022 | 0.803361 | 3.62E-49 | -1.69E-46 |
| MS.gene055890 | MS.gene068022 | 0.80354 | 3.32E-49 | -1.69E-46 |
| MS.gene055891 | MS.gene068022 | 0.838539 | 2.71E-57 | -1.69E-46 |
| MS.gene055979 | MS.gene068022 | 0.800852 | 1.19E-48 | -1.69E-46 |
| MS.gene056005 | MS.gene068022 | 0.842961 | 1.88E-58 | -1.69E-46 |
| MS.gene056043 | MS.gene068022 | -0.849607 | 2.90E-60 | -1.69E-46 |
| MS.gene056075 | MS.gene068022 | 0.81034 | 1.21E-50 | -1.69E-46 |
| MS.gene05608 | MS.gene068022 | 0.819712 | 1.01E-52 | -1.69E-46 |
| MS.gene056308 | MS.gene068022 | 0.851444 | 8.84E-61 | -1.69E-46 |
| MS.gene057294 | MS.gene068022 | 0.807035 | 6.15E-50 | -1.69E-46 |
| MS.gene057446 | MS.gene068022 | 0.809003 | 2.35E-50 | -1.69E-46 |
| MS.gene057705 | MS.gene068022 | 0.815273 | 1.01E-51 | -1.69E-46 |
| MS.gene058087 | MS.gene068022 | 0.804591 | 2.01E-49 | -1.69E-46 |
| MS.gene058339 | MS.gene068022 | 0.82976 | 4.30E-55 | -1.69E-46 |
| MS.gene058492 | MS.gene068022 | 0.801537 | 8.59E-49 | -1.69E-46 |
| MS.gene058852 | MS.gene068022 | 0.859057 | 5.38E-63 | -1.69E-46 |
| MS.gene058945 | MS.gene068022 | 0.850987 | 1.19E-60 | -1.69E-46 |
| MS.gene059171 | MS.gene068022 | 0.838202 | 3.31E-57 | -1.69E-46 |
| MS.gene059227 | MS.gene068022 | 0.825353 | 4.91E-54 | -1.69E-46 |
| MS.gene06001 | MS.gene068022 | 0.80318 | 3.94E-49 | -1.69E-46 |
| MS.gene060194 | MS.gene068022 | 0.818568 | 1.83E-52 | -1.69E-46 |
| MS.gene060429 | MS.gene068022 | 0.80808 | 3.69E-50 | -1.69E-46 |
| MS.gene060519 | MS.gene068022 | 0.840135 | 1.04E-57 | -1.69E-46 |
| MS.gene060587 | MS.gene068022 | 0.856516 | 3.05E-62 | -1.69E-46 |
| MS.gene060884 | MS.gene068022 | 0.833158 | 6.26E-56 | -1.69E-46 |
| MS.gene060886 | MS.gene068022 | 0.819851 | 9.35E-53 | -1.69E-46 |
| MS.gene061006 | MS.gene068022 | 0.85165 | 7.73E-61 | -1.69E-46 |
| MS.gene061100 | MS.gene068022 | 0.827699 | 1.35E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068022.t1 | MTR_4g093660 | 98.824 | 85 | 1 | 0 | 1 | 85 | 1 | 85 | 4.01e-58 | 172 |
| MS.gene068022.t1 | MTR_2g009660 | 85.882 | 85 | 12 | 0 | 1 | 85 | 63 | 147 | 2.55e-50 | 155 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068022.t1 | AT4G33640 | 80.769 | 78 | 15 | 0 | 1 | 78 | 1 | 78 | 1.09e-41 | 132 |
| MS.gene068022.t1 | AT4G33640 | 80.769 | 78 | 15 | 0 | 1 | 78 | 67 | 144 | 3.34e-41 | 132 |
Find 17 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCATTGTGAAGGTCACATT+TGG | 0.398089 | 4.3:-20897256 | MS.gene068022:intron |
| GAGTGATTTCCACATTATCA+TGG | 0.446001 | 4.3:+20897116 | None:intergenic |
| GAACACTCTTTCACGATGAT+CGG | 0.491735 | 4.3:-20897234 | MS.gene068022:CDS |
| AAGAGGAGGTTGAAAAGCTG+AGG | 0.496528 | 4.3:-20898003 | MS.gene068022:CDS |
| CCGAATCAGTTAGCAGCAGC+AGG | 0.502169 | 4.3:+20897082 | None:intergenic |
| CAGTTAGCAGCAGCAGGAGC+AGG | 0.504867 | 4.3:+20897088 | None:intergenic |
| CTTGGAAATGTTCAAGCAGA+TGG | 0.516658 | 4.3:-20897965 | MS.gene068022:CDS |
| AGGGAAGAAATCAAGAGGCT+TGG | 0.527446 | 4.3:-20897983 | MS.gene068022:CDS |
| TGTGAAGATGAACGTAGAAG+AGG | 0.563453 | 4.3:-20898020 | None:intergenic |
| GAAGATGAACGTAGAAGAGG+AGG | 0.565552 | 4.3:-20898017 | None:intergenic |
| AGAGGAGGTTGAAAAGCTGA+GGG | 0.572997 | 4.3:-20898002 | MS.gene068022:CDS |
| AATATATTTGAAGCACTCGT+CGG | 0.601303 | 4.3:-20897205 | MS.gene068022:CDS |
| TCAAGCAGATGGTTCTTGCA+AGG | 0.604584 | 4.3:-20897954 | MS.gene068022:intron |
| AGCTGAGGGAAGAAATCAAG+AGG | 0.626324 | 4.3:-20897988 | MS.gene068022:CDS |
| GACGGTGAACTACTGTTGCA+AGG | 0.632747 | 4.3:-20897142 | MS.gene068022:CDS |
| GCAAGGAGTCCATGATAATG+TGG | 0.676630 | 4.3:-20897125 | MS.gene068022:CDS |
| CGTAAAGTAGTTGCATACGA+CGG | 0.749247 | 4.3:-20897160 | MS.gene068022:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATAAACAAATTAATGTAT+AGG | + | chr4.3:20897458-20897477 | None:intergenic | 10.0% |
| !! | ATAATTGTAATTAAATGTGT+TGG | - | chr4.3:20897630-20897649 | MS.gene068022:intron | 15.0% |
| !! | TATTATTGATATGATGTTTA+GGG | + | chr4.3:20897592-20897611 | None:intergenic | 15.0% |
| !!! | AATTTGTTTATTATAAACCA+TGG | - | chr4.3:20897465-20897484 | MS.gene068022:intron | 15.0% |
| !! | GAATCAAGTTCTTAATTAAA+AGG | + | chr4.3:20897252-20897271 | None:intergenic | 20.0% |
| !! | GTATTATTGATATGATGTTT+AGG | + | chr4.3:20897593-20897612 | None:intergenic | 20.0% |
| !! | TTTCATATGATATTTCGTAA+TGG | + | chr4.3:20897385-20897404 | None:intergenic | 20.0% |
| ! | AAATATGTAATGCATTGTGA+AGG | - | chr4.3:20897834-20897853 | MS.gene068022:intron | 25.0% |
| ! | ATGCATCATGAAAATGTATA+TGG | + | chr4.3:20897214-20897233 | None:intergenic | 25.0% |
| ! | CATTCCATATAAAACTGATA+TGG | - | chr4.3:20897511-20897530 | MS.gene068022:intron | 25.0% |
| ! | TAATGTATAGGTAGATACAT+TGG | + | chr4.3:20897446-20897465 | None:intergenic | 25.0% |
| ! | TCAGATCTAGATATATTGAT+GGG | - | chr4.3:20897270-20897289 | MS.gene068022:intron | 25.0% |
| ! | TTCAGATCTAGATATATTGA+TGG | - | chr4.3:20897269-20897288 | MS.gene068022:intron | 25.0% |
| ! | TTGTTTATTATAAACCATGG+AGG | - | chr4.3:20897468-20897487 | MS.gene068022:intron | 25.0% |
| !! | GAAAATGTATATGGTTTGTA+AGG | + | chr4.3:20897205-20897224 | None:intergenic | 25.0% |
| !!! | AATTTGCTGAAATAGTACTA+TGG | - | chr4.3:20897690-20897709 | MS.gene068022:intron | 25.0% |
| !!! | CATATCAGTTTTATATGGAA+TGG | + | chr4.3:20897513-20897532 | None:intergenic | 25.0% |
| TATAGTGAGTGATGGTTAAT+TGG | - | chr4.3:20897740-20897759 | MS.gene068022:intron | 30.0% | |
| TTCTTGCAAGGTATAACAAA+AGG | - | chr4.3:20897158-20897177 | MS.gene068022:CDS | 30.0% | |
| ! | AATATATTTGAAGCACTCGT+CGG | - | chr4.3:20897895-20897914 | MS.gene068022:intron | 30.0% |
| ! | CTATATCTTCAGTTGATTCA+TGG | + | chr4.3:20897725-20897744 | None:intergenic | 30.0% |
| ! | TGATGGGTATTTATTGAATG+TGG | - | chr4.3:20897286-20897305 | MS.gene068022:intron | 30.0% |
| ! | TGGTCACTTTCTTATTCTTA+TGG | - | chr4.3:20897306-20897325 | MS.gene068022:intron | 30.0% |
| !! | CTAGCCATATCAGTTTTATA+TGG | + | chr4.3:20897518-20897537 | None:intergenic | 30.0% |
| ACTGAAGATATAGTGAGTGA+TGG | - | chr4.3:20897732-20897751 | MS.gene068022:intron | 35.0% | |
| GAGTGATTTCCACATTATCA+TGG | + | chr4.3:20897987-20898006 | None:intergenic | 35.0% | |
| GTTAATGCGCATATGTGAAT+TGG | - | chr4.3:20897770-20897789 | MS.gene068022:intron | 35.0% | |
| TCTTATGGATCTAGATCACA+AGG | - | chr4.3:20897321-20897340 | MS.gene068022:intron | 35.0% | |
| CACAAGGTCATTCACAACAT+TGG | - | chr4.3:20897337-20897356 | MS.gene068022:intron | 40.0% | |
| CGTAAAGTAGTTGCATACGA+CGG | - | chr4.3:20897940-20897959 | MS.gene068022:intron | 40.0% | |
| CTTGGAAATGTTCAAGCAGA+TGG | - | chr4.3:20897135-20897154 | MS.gene068022:CDS | 40.0% | |
| GAACACTCTTTCACGATGAT+CGG | - | chr4.3:20897866-20897885 | MS.gene068022:intron | 40.0% | |
| TGCATTGTGAAGGTCACATT+TGG | - | chr4.3:20897844-20897863 | MS.gene068022:intron | 40.0% | |
| AAGAGGAGGTTGAAAAGCTG+AGG | - | chr4.3:20897097-20897116 | MS.gene068022:CDS | 45.0% | |
| AGAGGAGGTTGAAAAGCTGA+GGG | - | chr4.3:20897098-20897117 | MS.gene068022:CDS | 45.0% | |
| AGCTGAGGGAAGAAATCAAG+AGG | - | chr4.3:20897112-20897131 | MS.gene068022:CDS | 45.0% | |
| AGGGAAGAAATCAAGAGGCT+TGG | - | chr4.3:20897117-20897136 | MS.gene068022:CDS | 45.0% | |
| TCAAGCAGATGGTTCTTGCA+AGG | - | chr4.3:20897146-20897165 | MS.gene068022:CDS | 45.0% | |
| ! | GCAAGGAGTCCATGATAATG+TGG | - | chr4.3:20897975-20897994 | MS.gene068022:CDS | 45.0% |
| CGCATGATATCTCTCCTCCA+TGG | + | chr4.3:20897485-20897504 | None:intergenic | 50.0% | |
| GACGGTGAACTACTGTTGCA+AGG | - | chr4.3:20897958-20897977 | MS.gene068022:CDS | 50.0% | |
| CAGTTAGCAGCAGCAGGAGC+AGG | + | chr4.3:20898015-20898034 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 20897087 | 20898035 | 20897087 | ID=MS.gene068022 |
| chr4.3 | mRNA | 20897087 | 20898035 | 20897087 | ID=MS.gene068022.t1;Parent=MS.gene068022 |
| chr4.3 | exon | 20897955 | 20898035 | 20897955 | ID=MS.gene068022.t1.exon1;Parent=MS.gene068022.t1 |
| chr4.3 | CDS | 20897955 | 20898035 | 20897955 | ID=cds.MS.gene068022.t1;Parent=MS.gene068022.t1 |
| chr4.3 | exon | 20897087 | 20897266 | 20897087 | ID=MS.gene068022.t1.exon2;Parent=MS.gene068022.t1 |
| chr4.3 | CDS | 20897087 | 20897266 | 20897087 | ID=cds.MS.gene068022.t1;Parent=MS.gene068022.t1 |
| Gene Sequence |
| Protein sequence |
