AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070694


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070694.t1 MTR_1g032560 94.186 172 10 0 1 172 1 172 4.45e-112 316
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070694.t1 AT2G01755 58.475 118 49 0 54 171 56 173 1.58e-42 140
MS.gene070694.t1 AT2G01755 58.475 118 49 0 54 171 62 179 1.97e-42 140

Find 46 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGTCTATAGATAGAATAAA+AGG 0.213144 1.4:+17086543 MS.gene070694:CDS
ATCAAAGAGGTATAAGCTTT+TGG 0.314010 1.4:+17086032 MS.gene070694:CDS
TAAGTACTTCTACTTCAATT+TGG 0.331886 1.4:-17086198 None:intergenic
GGAAACTTGTCAATTGATAA+TGG 0.335882 1.4:-17085959 None:intergenic
CCATGGCATCATTGTCCATT+TGG 0.363669 1.4:-17085921 None:intergenic
AGAATGCTTCTTGTTGGGTC+AGG 0.383377 1.4:-17086501 None:intergenic
GAAGATAGAGATGATGAGTA+TGG 0.389990 1.4:+17086094 MS.gene070694:CDS
GCTGGAAGAATGCTTCTTGT+TGG 0.394629 1.4:-17086507 None:intergenic
CTTCTGCAGATACTCAATAT+TGG 0.406034 1.4:-17086580 None:intergenic
CTGGAAGAATGCTTCTTGTT+GGG 0.406470 1.4:-17086506 None:intergenic
GACTTTATCACTGCCCTGGC+TGG 0.412026 1.4:-17086525 None:intergenic
TTGTCAATTGATAATGGTAT+AGG 0.420243 1.4:-17085953 None:intergenic
AGTAGAAGTACTTATCTTCA+AGG 0.426944 1.4:+17086207 MS.gene070694:CDS
TGTCCATTTGGCCATACCCT+TGG 0.430122 1.4:-17085909 None:intergenic
TCAATATTGGCAGGGTCAAA+AGG 0.439860 1.4:-17086567 None:intergenic
GTCCATTTGGCCATACCCTT+GGG 0.442034 1.4:-17085908 None:intergenic
CAATGAGGAAATTTCAAAGT+TGG 0.445765 1.4:+17086120 MS.gene070694:CDS
CTCTATCTTCATCCTCATCT+TGG 0.448503 1.4:-17086081 None:intergenic
TGCAGATACTCAATATTGGC+AGG 0.450870 1.4:-17086576 None:intergenic
TTCCCAAGGGTATGGCCAAA+TGG 0.469349 1.4:+17085906 MS.gene070694:CDS
ATACCCTTGGGAAGTGTAGT+AGG 0.478990 1.4:-17085896 None:intergenic
GGACAATGATGCCATGGCCT+AGG 0.481592 1.4:+17085927 MS.gene070694:CDS
CAAGAAGCATTCTTCCAGCC+AGG 0.483504 1.4:+17086511 MS.gene070694:CDS
GAATGCTTCTTGTTGGGTCA+GGG 0.497042 1.4:-17086500 None:intergenic
CACAAGCTTACATTGTGAGT+TGG 0.506810 1.4:-17085980 None:intergenic
AGTCTATAGATAGAATAAAA+GGG 0.523947 1.4:+17086544 MS.gene070694:CDS
AATGCTTCTTGTTGGGTCAG+GGG 0.544161 1.4:-17086499 None:intergenic
TACTACACTTCCCAAGGGTA+TGG 0.545556 1.4:+17085898 MS.gene070694:CDS
TTTGTCTTGAATTCTTCCCA+TGG 0.554335 1.4:-17086609 None:intergenic
ATCTGCAGAAGGATGTTCCA+TGG 0.555118 1.4:+17086592 MS.gene070694:CDS
GATAATGGTATAGGTAGCCT+AGG 0.559854 1.4:-17085944 None:intergenic
TATAGACTTTATCACTGCCC+TGG 0.571801 1.4:-17086529 None:intergenic
ACTCATTGTTCATGCACTTG+TGG 0.576626 1.4:+17086174 MS.gene070694:CDS
GCATCCTACTACACTTCCCA+AGG 0.594031 1.4:+17085892 MS.gene070694:CDS
AAGAAGCATTCTTCCAGCCA+GGG 0.602074 1.4:+17086512 MS.gene070694:CDS
TTCTATAGTCAATCAGCAAG+AGG 0.607447 1.4:+17086145 MS.gene070694:CDS
AGGGGAAGTACCATAGCTCA+AGG 0.608247 1.4:-17086481 None:intergenic
TCTGCAGAAGGATGTTCCAT+GGG 0.613447 1.4:+17086593 MS.gene070694:CDS
CAATATTGAGTATCTGCAGA+AGG 0.617485 1.4:+17086581 MS.gene070694:CDS
CATCCTACTACACTTCCCAA+GGG 0.625588 1.4:+17085893 MS.gene070694:CDS
CCAAATGGACAATGATGCCA+TGG 0.626699 1.4:+17085921 MS.gene070694:CDS
GGTATAGGTAGCCTAGGCCA+TGG 0.632885 1.4:-17085938 None:intergenic
GCAAATTAGATACCAAGATG+AGG 0.635629 1.4:+17086069 MS.gene070694:CDS
TTAGATTTGAGCAATCAAAG+AGG 0.645074 1.4:+17086019 MS.gene070694:CDS
GCAGATACTCAATATTGGCA+GGG 0.648369 1.4:-17086575 None:intergenic
TGATGAGTATGGACACAATG+AGG 0.651393 1.4:+17086105 MS.gene070694:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATAATTTGTTTGTTTTAT+TGG - chr1.4:17086286-17086305 None:intergenic 10.0%
!!! TTTTTTTTCTAATTAGAAAA+AGG + chr1.4:17086327-17086346 MS.gene070694:intron 10.0%
!! AAGTCTATAGATAGAATAAA+AGG + chr1.4:17086543-17086562 MS.gene070694:CDS 20.0%
!! AGTCTATAGATAGAATAAAA+GGG + chr1.4:17086544-17086563 MS.gene070694:CDS 20.0%
!!! TTGTTTGTTTTATTGGTTAT+TGG - chr1.4:17086279-17086298 None:intergenic 20.0%
! CCATTTGCAAAACAAAAATT+TGG - chr1.4:17086384-17086403 None:intergenic 25.0%
! TAAGTACTTCTACTTCAATT+TGG - chr1.4:17086201-17086220 None:intergenic 25.0%
!!! CCAAATTTTTGTTTTGCAAA+TGG + chr1.4:17086381-17086400 MS.gene070694:intron 25.0%
!!! TTGTCAATTGATAATGGTAT+AGG - chr1.4:17085956-17085975 None:intergenic 25.0%
ACTTATCTTCAAGGTAAGTT+TGG + chr1.4:17086216-17086235 MS.gene070694:intron 30.0%
CAATGAGGAAATTTCAAAGT+TGG + chr1.4:17086120-17086139 MS.gene070694:CDS 30.0%
GGAAACTTGTCAATTGATAA+TGG - chr1.4:17085962-17085981 None:intergenic 30.0%
TGACAAACCATTTCATTTCA+GGG + chr1.4:17086447-17086466 MS.gene070694:intron 30.0%
TTAGATTTGAGCAATCAAAG+AGG + chr1.4:17086019-17086038 MS.gene070694:CDS 30.0%
!! AGTAGAAGTACTTATCTTCA+AGG + chr1.4:17086207-17086226 MS.gene070694:CDS 30.0%
!! ATCAAAGAGGTATAAGCTTT+TGG + chr1.4:17086032-17086051 MS.gene070694:CDS 30.0%
AGAAAACCCCTGAAATGAAA+TGG - chr1.4:17086457-17086476 None:intergenic 35.0%
CAATATTGAGTATCTGCAGA+AGG + chr1.4:17086581-17086600 MS.gene070694:CDS 35.0%
CTTCTGCAGATACTCAATAT+TGG - chr1.4:17086583-17086602 None:intergenic 35.0%
GAAGATAGAGATGATGAGTA+TGG + chr1.4:17086094-17086113 MS.gene070694:CDS 35.0%
GACAAACCATTTCATTTCAG+GGG + chr1.4:17086448-17086467 MS.gene070694:intron 35.0%
GCAAATTAGATACCAAGATG+AGG + chr1.4:17086069-17086088 MS.gene070694:CDS 35.0%
GTGACAAACCATTTCATTTC+AGG + chr1.4:17086446-17086465 MS.gene070694:intron 35.0%
TTCTATAGTCAATCAGCAAG+AGG + chr1.4:17086145-17086164 MS.gene070694:CDS 35.0%
TTTGTCTTGAATTCTTCCCA+TGG - chr1.4:17086612-17086631 None:intergenic 35.0%
! TTTTCTTCATCCTTGAGCTA+TGG + chr1.4:17086471-17086490 MS.gene070694:CDS 35.0%
ACTCATTGTTCATGCACTTG+TGG + chr1.4:17086174-17086193 MS.gene070694:CDS 40.0%
CACAAGCTTACATTGTGAGT+TGG - chr1.4:17085983-17086002 None:intergenic 40.0%
CTCTATCTTCATCCTCATCT+TGG - chr1.4:17086084-17086103 None:intergenic 40.0%
CTGGAAGAATGCTTCTTGTT+GGG - chr1.4:17086509-17086528 None:intergenic 40.0%
GCAGATACTCAATATTGGCA+GGG - chr1.4:17086578-17086597 None:intergenic 40.0%
TATAGACTTTATCACTGCCC+TGG - chr1.4:17086532-17086551 None:intergenic 40.0%
TCAATATTGGCAGGGTCAAA+AGG - chr1.4:17086570-17086589 None:intergenic 40.0%
TGATGAGTATGGACACAATG+AGG + chr1.4:17086105-17086124 MS.gene070694:CDS 40.0%
TGCAGATACTCAATATTGGC+AGG - chr1.4:17086579-17086598 None:intergenic 40.0%
! GATAATGGTATAGGTAGCCT+AGG - chr1.4:17085947-17085966 None:intergenic 40.0%
!! AGAGGTATAAGCTTTTGGTG+TGG + chr1.4:17086037-17086056 MS.gene070694:CDS 40.0%
AATGCTTCTTGTTGGGTCAG+GGG - chr1.4:17086502-17086521 None:intergenic 45.0%
AGAATGCTTCTTGTTGGGTC+AGG - chr1.4:17086504-17086523 None:intergenic 45.0%
ATCTGCAGAAGGATGTTCCA+TGG + chr1.4:17086592-17086611 MS.gene070694:CDS 45.0%
CATCCTACTACACTTCCCAA+GGG + chr1.4:17085893-17085912 MS.gene070694:CDS 45.0%
CCAAATGGACAATGATGCCA+TGG + chr1.4:17085921-17085940 MS.gene070694:CDS 45.0%
CCATGGCATCATTGTCCATT+TGG - chr1.4:17085924-17085943 None:intergenic 45.0%
GAATGCTTCTTGTTGGGTCA+GGG - chr1.4:17086503-17086522 None:intergenic 45.0%
GCTGGAAGAATGCTTCTTGT+TGG - chr1.4:17086510-17086529 None:intergenic 45.0%
TACTACACTTCCCAAGGGTA+TGG + chr1.4:17085898-17085917 MS.gene070694:CDS 45.0%
TCTGCAGAAGGATGTTCCAT+GGG + chr1.4:17086593-17086612 MS.gene070694:CDS 45.0%
! ATACCCTTGGGAAGTGTAGT+AGG - chr1.4:17085899-17085918 None:intergenic 45.0%
!! AAGAAGCATTCTTCCAGCCA+GGG + chr1.4:17086512-17086531 MS.gene070694:CDS 45.0%
!! TAAGCTTTTGGTGTGGAGCA+AGG + chr1.4:17086044-17086063 MS.gene070694:CDS 45.0%
GCATCCTACTACACTTCCCA+AGG + chr1.4:17085892-17085911 MS.gene070694:CDS 50.0%
GTCCATTTGGCCATACCCTT+GGG - chr1.4:17085911-17085930 None:intergenic 50.0%
TGTCCATTTGGCCATACCCT+TGG - chr1.4:17085912-17085931 None:intergenic 50.0%
! TTCCCAAGGGTATGGCCAAA+TGG + chr1.4:17085906-17085925 MS.gene070694:CDS 50.0%
!! AGGGGAAGTACCATAGCTCA+AGG - chr1.4:17086484-17086503 None:intergenic 50.0%
!! CAAGAAGCATTCTTCCAGCC+AGG + chr1.4:17086511-17086530 MS.gene070694:CDS 50.0%
GACTTTATCACTGCCCTGGC+TGG - chr1.4:17086528-17086547 None:intergenic 55.0%
GGACAATGATGCCATGGCCT+AGG + chr1.4:17085927-17085946 MS.gene070694:CDS 55.0%
GGTATAGGTAGCCTAGGCCA+TGG - chr1.4:17085941-17085960 None:intergenic 55.0%
!! GCTTTTGGTGTGGAGCAAGG+AGG + chr1.4:17086047-17086066 MS.gene070694:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 17085890 17086647 17085890 ID=MS.gene070694
chr1.4 mRNA 17085890 17086647 17085890 ID=MS.gene070694.t1;Parent=MS.gene070694
chr1.4 exon 17085890 17086228 17085890 ID=MS.gene070694.t1.exon1;Parent=MS.gene070694.t1
chr1.4 CDS 17085890 17086228 17085890 ID=cds.MS.gene070694.t1;Parent=MS.gene070694.t1
chr1.4 exon 17086468 17086647 17086468 ID=MS.gene070694.t1.exon2;Parent=MS.gene070694.t1
chr1.4 CDS 17086468 17086647 17086468 ID=cds.MS.gene070694.t1;Parent=MS.gene070694.t1
Gene Sequence

>MS.gene070694

ATGCATCCTACTACACTTCCCAAGGGTATGGCCAAATGGACAATGATGCCATGGCCTAGGCTACCTATACCATTATCAATTGACAAGTTTCCAACTCACAATGTAAGCTTGTGTTTTGACTTGAAAGAATTAGATTTGAGCAATCAAAGAGGTATAAGCTTTTGGTGTGGAGCAAGGAGGCAAATTAGATACCAAGATGAGGATGAAGATAGAGATGATGAGTATGGACACAATGAGGAAATTTCAAAGTTGGAATTCTATAGTCAATCAGCAAGAGGAGAAGCACTCATTGTTCATGCACTTGTGGACCAAATTGAAGTAGAAGTACTTATCTTCAAGGTAAGTTTGGAATTTATGTCAATAATTGCTTTTAAGAGTCTTGCTATCCAATAACCAATAAAACAAACAAATTATTTAAAAATATATGAATCTACTTCTTTTTTTTCTAATTAGAAAAAGGTAAGAAGCACATTAAAATTCTTGTGATTTTACCAAATTTTTGTTTTGCAAATGGTATGCTGATTACTCTAAAAATATATTTCTTGATAGAAATTTAGTGACAAACCATTTCATTTCAGGGGTTTTCTTCATCCTTGAGCTATGGTACTTCCCCTGACCCAACAAGAAGCATTCTTCCAGCCAGGGCAGTGATAAAGTCTATAGATAGAATAAAAGGGCCTTTTGACCCTGCCAATATTGAGTATCTGCAGAAGGATGTTCCATGGGAAGAATTCAAGACAAATATTCTTTCTAGCTAA

Protein sequence

>MS.gene070694.t1

MHPTTLPKGMAKWTMMPWPRLPIPLSIDKFPTHNVSLCFDLKELDLSNQRGISFWCGARRQIRYQDEDEDRDDEYGHNEEISKLEFYSQSARGEALIVHALVDQIEVEVLIFKGFSSSLSYGTSPDPTRSILPARAVIKSIDRIKGPFDPANIEYLQKDVPWEEFKTNILSS