AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073894


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073894.t1 MTR_1g045140 96.850 127 4 0 1 127 1 127 3.94e-89 254
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073894.t1 AT2G05620 75.940 133 23 4 2 127 3 133 8.71e-66 196
MS.gene073894.t1 AT2G05620 75.940 133 23 4 2 127 3 133 8.71e-66 196

Find 29 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACGTTTCAATCAGCTTAG+AGG 0.319386 1.3:-26742516 MS.gene073894:CDS
TCATTAACATTCTTCATCAT+AGG 0.355579 1.3:+26742594 None:intergenic
ACTGAATTCTGCAAATCAAT+AGG 0.397917 1.3:-26741340 MS.gene073894:CDS
AACAGAGACAAGGGCTGATT+AGG 0.411490 1.3:-26741306 MS.gene073894:CDS
TGTGTTGGTTCATCATTCTA+TGG 0.416628 1.3:-26742720 MS.gene073894:CDS
GCCACTTCAATCTCTGCAAC+AGG 0.418962 1.3:-26742744 MS.gene073894:CDS
ATCTCTGCAACAGGTTGTGT+TGG 0.422671 1.3:-26742735 MS.gene073894:CDS
TTACGAGTAACCACAACAAT+TGG 0.430918 1.3:+26742552 None:intergenic
AGCCATTGCTTTACACTCTC+AGG 0.432190 1.3:-26742490 MS.gene073894:intron
GTTCATCATTCTATGGAAGC+TGG 0.445752 1.3:-26742713 MS.gene073894:CDS
GTTTCTTCGCAAGTTCGTAT+TGG 0.470806 1.3:-26742645 MS.gene073894:CDS
GTGGTTACTCGTAATATTGT+TGG 0.474864 1.3:-26742543 MS.gene073894:CDS
ATCATAGGTTGCAACCTCAC+TGG 0.491055 1.3:+26742609 None:intergenic
TTCATCATTCTATGGAAGCT+GGG 0.491238 1.3:-26742712 MS.gene073894:CDS
ATTAGGTTGGCTAAGAAGAA+TGG 0.501313 1.3:-26741289 MS.gene073894:CDS
ATGATGAAGAATGTTAATGA+AGG 0.502442 1.3:-26742591 MS.gene073894:CDS
AGCTGGGGAACATCTATAGT+TGG 0.521344 1.3:-26742696 MS.gene073894:CDS
AAGAAGAATGGAGAATGGCT+TGG 0.545873 1.3:-26741277 MS.gene073894:CDS
AAGAATGTTAATGAAGGGAA+AGG 0.566696 1.3:-26742585 MS.gene073894:CDS
GAGACAAGGGCTGATTAGGT+TGG 0.587941 1.3:-26741302 MS.gene073894:CDS
TGGTGAAGATTACAGCATGT+TGG 0.600796 1.3:-26742676 MS.gene073894:CDS
TGGCTAAGAAGAATGGAGAA+TGG 0.601122 1.3:-26741282 MS.gene073894:CDS
TGATGAAGAATGTTAATGAA+GGG 0.601637 1.3:-26742590 MS.gene073894:CDS
CACCTGAGAGTGTAAAGCAA+TGG 0.607087 1.3:+26742488 None:intergenic
ACCTGTTGCAGAGATTGAAG+TGG 0.616422 1.3:+26742743 None:intergenic
TCATCATTCTATGGAAGCTG+GGG 0.629743 1.3:-26742711 MS.gene073894:CDS
GCAGATGCAAAACAGAGACA+AGG 0.641927 1.3:-26741316 MS.gene073894:CDS
CAGATGCAAAACAGAGACAA+GGG 0.659781 1.3:-26741315 MS.gene073894:CDS
GAAGAGTTAGTAAGCCAGTG+AGG 0.661265 1.3:-26742623 MS.gene073894:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACTTATTTAAAAAATTTGT+GGG - chr1.3:26741790-26741809 MS.gene073894:intron 10.0%
!! TTACTTATTTAAAAAATTTG+TGG - chr1.3:26741789-26741808 MS.gene073894:intron 10.0%
!! ATTCAAAAAAACACATTTCT+AGG - chr1.3:26741932-26741951 MS.gene073894:intron 20.0%
!! ATTTGTGTGAATAAAATTGA+TGG - chr1.3:26742589-26742608 MS.gene073894:CDS 20.0%
!! TAAAACTAAAGAATTTGAGA+TGG - chr1.3:26741974-26741993 MS.gene073894:intron 20.0%
!! TCAAGTACAATATATCAAAA+GGG - chr1.3:26741748-26741767 MS.gene073894:intron 20.0%
!! TTCAAAAAAACACATTTCTA+GGG - chr1.3:26741933-26741952 MS.gene073894:intron 20.0%
!! TTCAAGTACAATATATCAAA+AGG - chr1.3:26741747-26741766 MS.gene073894:intron 20.0%
!! TTGTTAATTAAAGTGATACT+AGG - chr1.3:26741826-26741845 MS.gene073894:intron 20.0%
!!! AAACTAAATTTTTTCTCTCA+AGG - chr1.3:26741850-26741869 MS.gene073894:intron 20.0%
!!! TAGTTTATAGTTGATATTTG+CGG - chr1.3:26742033-26742052 MS.gene073894:intron 20.0%
!!! TTATATGATTCTCTAGAATT+AGG - chr1.3:26741654-26741673 MS.gene073894:intron 20.0%
!!! TTTCATATTTTGAAATCTGT+AGG - chr1.3:26742643-26742662 MS.gene073894:CDS 20.0%
! ATGATGAAGAATGTTAATGA+AGG - chr1.3:26741420-26741439 MS.gene073894:intron 25.0%
! ATGTGGTTTATATGTTTCAA+TGG - chr1.3:26741682-26741701 MS.gene073894:intron 25.0%
! CTAACATATGATAAGAGATT+GGG - chr1.3:26742401-26742420 MS.gene073894:intron 25.0%
! GAAATTTATTGTCACAACTT+AGG - chr1.3:26741620-26741639 MS.gene073894:intron 25.0%
! GAATAAAATTGATGGAAGAA+TGG - chr1.3:26742597-26742616 MS.gene073894:CDS 25.0%
! TAACATATGATAAGAGATTG+GGG - chr1.3:26742402-26742421 MS.gene073894:intron 25.0%
! TCATTAACATTCTTCATCAT+AGG + chr1.3:26741420-26741439 None:intergenic 25.0%
! TCTAACATATGATAAGAGAT+TGG - chr1.3:26742400-26742419 MS.gene073894:intron 25.0%
! TGATGAAATATGTTGAAAAG+AGG - chr1.3:26741724-26741743 MS.gene073894:intron 25.0%
! TGATGAAGAATGTTAATGAA+GGG - chr1.3:26741421-26741440 MS.gene073894:intron 25.0%
!! TCAAAAAAACACATTTCTAG+GGG - chr1.3:26741934-26741953 MS.gene073894:intron 25.0%
!! TCTAGAATTAGGATTATATG+TGG - chr1.3:26741665-26741684 MS.gene073894:intron 25.0%
!!! ATAATTAGTGCTTTCTAGTT+GGG + chr1.3:26742381-26742400 None:intergenic 25.0%
!!! GCATAGCTTTTTTTTTTTTG+AGG - chr1.3:26741592-26741611 MS.gene073894:intron 25.0%
!!! TTTTTTCTCTCAAGGTTATA+AGG - chr1.3:26741858-26741877 MS.gene073894:intron 25.0%
AAGAATGTTAATGAAGGGAA+AGG - chr1.3:26741426-26741445 MS.gene073894:intron 30.0%
ACTGAATTCTGCAAATCAAT+AGG - chr1.3:26742671-26742690 MS.gene073894:CDS 30.0%
GATTTGAACTTCATCTCTTA+AGG + chr1.3:26742507-26742526 None:intergenic 30.0%
GTTTAAAGATTGATGGAAGA+TGG - chr1.3:26742140-26742159 MS.gene073894:intron 30.0%
TGTGGTTGTTTAAAGATTGA+TGG - chr1.3:26742133-26742152 MS.gene073894:intron 30.0%
TTGATATTTGCGGAGAATTA+TGG - chr1.3:26742043-26742062 MS.gene073894:intron 30.0%
! GATAATTAGTGCTTTCTAGT+TGG + chr1.3:26742382-26742401 None:intergenic 30.0%
!! TAATTAGTGCTTTCTAGTTG+GGG + chr1.3:26742380-26742399 None:intergenic 30.0%
!! TATTTTTGCTCCAATTGTTG+TGG - chr1.3:26741449-26741468 MS.gene073894:intron 30.0%
!! TCAATGGTTTGTTAGATTGA+AGG - chr1.3:26741698-26741717 MS.gene073894:intron 30.0%
!!! TTTGAGATGGTTTTGAATCA+AGG - chr1.3:26741987-26742006 MS.gene073894:intron 30.0%
AAACGTTTCAATCAGCTTAG+AGG - chr1.3:26741495-26741514 MS.gene073894:intron 35.0%
AGTTGGGGAAATACTTGATT+GGG + chr1.3:26742365-26742384 None:intergenic 35.0%
ATGTTCGGTAATTATCCACA+AGG - chr1.3:26742448-26742467 MS.gene073894:intron 35.0%
GTGGTTACTCGTAATATTGT+TGG - chr1.3:26741468-26741487 MS.gene073894:intron 35.0%
TAGTTGGGGAAATACTTGAT+TGG + chr1.3:26742366-26742385 None:intergenic 35.0%
TCGGGATGTTTCAAAATGTT+CGG - chr1.3:26742433-26742452 MS.gene073894:intron 35.0%
TCTATGAGATGTCATCTCAA+TGG + chr1.3:26741895-26741914 None:intergenic 35.0%
TTACGAGTAACCACAACAAT+TGG + chr1.3:26741462-26741481 None:intergenic 35.0%
TTCATCATTCTATGGAAGCT+GGG - chr1.3:26741299-26741318 MS.gene073894:CDS 35.0%
TTGAGATGACATCTCATAGA+TGG - chr1.3:26741895-26741914 MS.gene073894:intron 35.0%
!! ATTAGGTTGGCTAAGAAGAA+TGG - chr1.3:26742722-26742741 MS.gene073894:CDS 35.0%
!! TGTGTTGGTTCATCATTCTA+TGG - chr1.3:26741291-26741310 MS.gene073894:CDS 35.0%
AAGAAGAATGGAGAATGGCT+TGG - chr1.3:26742734-26742753 MS.gene073894:CDS 40.0%
ACCACATACAAGAGACATCA+AGG + chr1.3:26742119-26742138 None:intergenic 40.0%
ACCTTGATGTCTCTTGTATG+TGG - chr1.3:26742115-26742134 MS.gene073894:intron 40.0%
AGATGACATCTCATAGATGG+AGG - chr1.3:26741898-26741917 MS.gene073894:intron 40.0%
AGTTCAAATCACCTCAGAGA+TGG - chr1.3:26742517-26742536 MS.gene073894:CDS 40.0%
CAAGGACGTGGTAATATTCA+TGG - chr1.3:26742005-26742024 MS.gene073894:intron 40.0%
CAGATGCAAAACAGAGACAA+GGG - chr1.3:26742696-26742715 MS.gene073894:CDS 40.0%
CTAGAAGAGAAAGTCTTGCT+TGG - chr1.3:26742204-26742223 MS.gene073894:intron 40.0%
CTGCAATTATTGTCGTGTTG+CGG - chr1.3:26742076-26742095 MS.gene073894:intron 40.0%
GCAAGACTTTCTCTTCTAGT+TGG + chr1.3:26742203-26742222 None:intergenic 40.0%
GTTCATCATTCTATGGAAGC+TGG - chr1.3:26741298-26741317 MS.gene073894:CDS 40.0%
GTTTCTTCGCAAGTTCGTAT+TGG - chr1.3:26741366-26741385 MS.gene073894:intron 40.0%
TCATCATTCTATGGAAGCTG+GGG - chr1.3:26741300-26741319 MS.gene073894:CDS 40.0%
TGGCTAAGAAGAATGGAGAA+TGG - chr1.3:26742729-26742748 MS.gene073894:CDS 40.0%
TGGTGAAGATTACAGCATGT+TGG - chr1.3:26741335-26741354 MS.gene073894:CDS 40.0%
TTTGTGTGTGAATGCCTAAG+TGG + chr1.3:26742249-26742268 None:intergenic 40.0%
!! ACTTTTTGAGCACACCACTT+AGG - chr1.3:26742232-26742251 MS.gene073894:intron 40.0%
!!! ATGGTTTTGAATCAAGGACG+TGG - chr1.3:26741993-26742012 MS.gene073894:intron 40.0%
AACAGAGACAAGGGCTGATT+AGG - chr1.3:26742705-26742724 MS.gene073894:CDS 45.0%
ACCTGTTGCAGAGATTGAAG+TGG + chr1.3:26741271-26741290 None:intergenic 45.0%
AGCCATTGCTTTACACTCTC+AGG - chr1.3:26741521-26741540 MS.gene073894:intron 45.0%
AGCTGGGGAACATCTATAGT+TGG - chr1.3:26741315-26741334 MS.gene073894:CDS 45.0%
ATCATAGGTTGCAACCTCAC+TGG + chr1.3:26741405-26741424 None:intergenic 45.0%
ATCTCTGCAACAGGTTGTGT+TGG - chr1.3:26741276-26741295 MS.gene073894:CDS 45.0%
GAAGAGTTAGTAAGCCAGTG+AGG - chr1.3:26741388-26741407 MS.gene073894:intron 45.0%
GACATCTCATAGATGGAGGA+GGG - chr1.3:26741902-26741921 MS.gene073894:intron 45.0%
GATAAGAGATTGGGGATGTG+TGG - chr1.3:26742410-26742429 MS.gene073894:intron 45.0%
GCAGATGCAAAACAGAGACA+AGG - chr1.3:26742695-26742714 MS.gene073894:CDS 45.0%
TGACATCTCATAGATGGAGG+AGG - chr1.3:26741901-26741920 MS.gene073894:intron 45.0%
! CACCTGAGAGTGTAAAGCAA+TGG + chr1.3:26741526-26741545 None:intergenic 45.0%
!! TTTCTAGGGGTGTTGAGACA+TGG - chr1.3:26741947-26741966 MS.gene073894:intron 45.0%
AGAGATTGGGGATGTGTGGT+CGG - chr1.3:26742414-26742433 MS.gene073894:intron 50.0%
CCATCATACGACCATCTCTG+AGG + chr1.3:26742531-26742550 None:intergenic 50.0%
CCTCAGAGATGGTCGTATGA+TGG - chr1.3:26742528-26742547 MS.gene073894:CDS 50.0%
GAGACAAGGGCTGATTAGGT+TGG - chr1.3:26742709-26742728 MS.gene073894:CDS 50.0%
GCCACTTCAATCTCTGCAAC+AGG - chr1.3:26741267-26741286 MS.gene073894:CDS 50.0%
ACCACTGAGCTGACACCTTG+TGG + chr1.3:26742466-26742485 None:intergenic 55.0%
GAGATTGGGGATGTGTGGTC+GGG - chr1.3:26742415-26742434 MS.gene073894:intron 55.0%
! TCCACAAGGTGTCAGCTCAG+TGG - chr1.3:26742462-26742481 MS.gene073894:intron 55.0%
!! CTAGGGGTGTTGAGACATGG+AGG - chr1.3:26741950-26741969 MS.gene073894:intron 55.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 26741264 26742769 26741264 ID=MS.gene073894
chr1.3 mRNA 26741264 26742769 26741264 ID=MS.gene073894.t1;Parent=MS.gene073894
chr1.3 exon 26742491 26742769 26742491 ID=MS.gene073894.t1.exon1;Parent=MS.gene073894.t1
chr1.3 CDS 26742491 26742769 26742491 ID=cds.MS.gene073894.t1;Parent=MS.gene073894.t1
chr1.3 exon 26741264 26741368 26741264 ID=MS.gene073894.t1.exon2;Parent=MS.gene073894.t1
chr1.3 CDS 26741264 26741368 26741264 ID=cds.MS.gene073894.t1;Parent=MS.gene073894.t1
Gene Sequence

>MS.gene073894

ATGGCCACTTCAATCTCTGCAACAGGTTGTGTTGGTTCATCATTCTATGGAAGCTGGGGAACATCTATAGTTGGTGAAGATTACAGCATGTTGGCTAAGTCAGTTTCTTCGCAAGTTCGTATTGGAAGAGTTAGTAAGCCAGTGAGGTTGCAACCTATGATGAAGAATGTTAATGAAGGGAAAGGTATTTTTGCTCCAATTGTTGTGGTTACTCGTAATATTGTTGGAAAGAAACGTTTCAATCAGCTTAGAGGCAAAGCCATTGCTTTACACTCTCAGGTGAATTAAATTCTATGTTCTTATTGTTTGTGTTGTTTACTATTCTTTTGCATAGCTTTTTTTTTTTTGAGGTGTTAGAAATTTATTGTCACAACTTAGGAACTTGTTTTTTTATATGATTCTCTAGAATTAGGATTATATGTGGTTTATATGTTTCAATGGTTTGTTAGATTGAAGGATTTGATGAAATATGTTGAAAAGAGGTTCAAGTACAATATATCAAAAGGGAAATGATTTTTTTTTTTTTTACTTATTTAAAAAATTTGTGGGAAGAGATAAATTATTGTTAATTAAAGTGATACTAGGTAAACTAAATTTTTTCTCTCAAGGTTATAAGGTTCAACTCTTACCATTGAGATGACATCTCATAGATGGAGGAGGGAGAATAGATTCAAAAAAACACATTTCTAGGGGTGTTGAGACATGGAGGATAAAACTAAAGAATTTGAGATGGTTTTGAATCAAGGACGTGGTAATATTCATGGTTGAGTAGTTTATAGTTGATATTTGCGGAGAATTATGGTTAAATGCAGCTGCAATTATTGTCGTGTTGCGGTCATAGAGACATCAAAACCTTGATGTCTCTTGTATGTGGTTGTTTAAAGATTGATGGAAGATGGAATAAACATATTTTTATGTAATACATATAATGAGAGACCAACTAGAAGAGAAAGTCTTGCTTGGACACAACTTTTTGAGCACACCACTTAGGCATTCACACACAAAATACATTTTATTTAATTATTTTCTCTCTCTATCTTTTTCTCAATTATATGTATGTGCGTGAGTGTGTCTAAAGTTTCTGACTAAAAACTTGTGCCCAATCAAGTATTTCCCCAACTAGAAAGCACTAATTATCTAACATATGATAAGAGATTGGGGATGTGTGGTCGGGATGTTTCAAAATGTTCGGTAATTATCCACAAGGTGTCAGCTCAGTGGTAAGAGTTTGATCTTTTAACCTTAAGAGATGAAGTTCAAATCACCTCAGAGATGGTCGTATGATGGTCATTAGTGTCACTTTATGTGAATAAATTTTATGAGACATTTGTGTGAATAAAATTGATGGAAGAATGGAACATAAATTTTCTGTACTTAACTTTCATATTTTGAAATCTGTAGGTAATTACTGAATTCTGCAAATCAATAGGAGCAGATGCAAAACAGAGACAAGGGCTGATTAGGTTGGCTAAGAAGAATGGAGAATGGCTTGGTTTTCTTGCATGA

Protein sequence

>MS.gene073894.t1

MATSISATGCVGSSFYGSWGTSIVGEDYSMLAKSVSSQVRIGRVSKPVRLQPMMKNVNEGKGIFAPIVVVTRNIVGKKRFNQLRGKAIALHSQVITEFCKSIGADAKQRQGLIRLAKKNGEWLGFLA