Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21644.t1 | XP_003611029.1 | 99 | 304 | 3 | 0 | 1 | 304 | 1 | 304 | 2.10E-175 | 624.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21644.t1 | Q40784 | 66.3 | 291 | 96 | 1 | 16 | 304 | 25 | 315 | 1.2e-118 | 427.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21644.t1 | G7KAP1 | 99.0 | 304 | 3 | 0 | 1 | 304 | 1 | 304 | 1.5e-175 | 624.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene21644 | 0.867966 | 9.26E-66 | -1.69E-46 |
| MS.gene049222 | MS.gene21644 | 0.831372 | 1.73E-55 | -1.69E-46 |
| MS.gene049285 | MS.gene21644 | 0.808632 | 2.82E-50 | -1.69E-46 |
| MS.gene049609 | MS.gene21644 | 0.849504 | 3.10E-60 | -1.69E-46 |
| MS.gene049805 | MS.gene21644 | 0.849966 | 2.30E-60 | -1.69E-46 |
| MS.gene050593 | MS.gene21644 | 0.817547 | 3.12E-52 | -1.69E-46 |
| MS.gene050747 | MS.gene21644 | 0.853061 | 3.06E-61 | -1.69E-46 |
| MS.gene050772 | MS.gene21644 | 0.839645 | 1.40E-57 | -1.69E-46 |
| MS.gene050950 | MS.gene21644 | 0.845496 | 3.92E-59 | -1.69E-46 |
| MS.gene05105 | MS.gene21644 | 0.824366 | 8.39E-54 | -1.69E-46 |
| MS.gene051167 | MS.gene21644 | -0.80027 | 1.56E-48 | -1.69E-46 |
| MS.gene051387 | MS.gene21644 | 0.829843 | 4.10E-55 | -1.69E-46 |
| MS.gene051666 | MS.gene21644 | 0.81183 | 5.75E-51 | -1.69E-46 |
| MS.gene051694 | MS.gene21644 | 0.818436 | 1.96E-52 | -1.69E-46 |
| MS.gene051858 | MS.gene21644 | 0.813698 | 2.24E-51 | -1.69E-46 |
| MS.gene052421 | MS.gene21644 | 0.860623 | 1.82E-63 | -1.69E-46 |
| MS.gene052501 | MS.gene21644 | 0.849801 | 2.56E-60 | -1.69E-46 |
| MS.gene052503 | MS.gene21644 | 0.823154 | 1.61E-53 | -1.69E-46 |
| MS.gene052505 | MS.gene21644 | 0.856595 | 2.89E-62 | -1.69E-46 |
| MS.gene052615 | MS.gene21644 | 0.83414 | 3.56E-56 | -1.69E-46 |
| MS.gene052823 | MS.gene21644 | 0.825462 | 4.63E-54 | -1.69E-46 |
| MS.gene052825 | MS.gene21644 | 0.825392 | 4.81E-54 | -1.69E-46 |
| MS.gene052982 | MS.gene21644 | 0.800303 | 1.54E-48 | -1.69E-46 |
| MS.gene053117 | MS.gene21644 | 0.804118 | 2.52E-49 | -1.69E-46 |
| MS.gene053213 | MS.gene21644 | 0.841219 | 5.43E-58 | -1.69E-46 |
| MS.gene053286 | MS.gene21644 | 0.830035 | 3.68E-55 | -1.69E-46 |
| MS.gene053421 | MS.gene21644 | -0.808656 | 2.78E-50 | -1.69E-46 |
| MS.gene053436 | MS.gene21644 | 0.802 | 6.90E-49 | -1.69E-46 |
| MS.gene053480 | MS.gene21644 | 0.806439 | 8.22E-50 | -1.69E-46 |
| MS.gene053574 | MS.gene21644 | 0.840655 | 7.63E-58 | -1.69E-46 |
| MS.gene053579 | MS.gene21644 | 0.81641 | 5.61E-52 | -1.69E-46 |
| MS.gene053626 | MS.gene21644 | 0.801519 | 8.67E-49 | -1.69E-46 |
| MS.gene053824 | MS.gene21644 | 0.813463 | 2.53E-51 | -1.69E-46 |
| MS.gene053873 | MS.gene21644 | 0.825535 | 4.45E-54 | -1.69E-46 |
| MS.gene054408 | MS.gene21644 | 0.83095 | 2.20E-55 | -1.69E-46 |
| MS.gene05505 | MS.gene21644 | 0.848693 | 5.21E-60 | -1.69E-46 |
| MS.gene055289 | MS.gene21644 | 0.809785 | 1.59E-50 | -1.69E-46 |
| MS.gene055502 | MS.gene21644 | 0.805238 | 1.47E-49 | -1.69E-46 |
| MS.gene055941 | MS.gene21644 | 0.815528 | 8.83E-52 | -1.69E-46 |
| MS.gene056511 | MS.gene21644 | 0.812042 | 5.17E-51 | -1.69E-46 |
| MS.gene056692 | MS.gene21644 | 0.804885 | 1.74E-49 | -1.69E-46 |
| MS.gene057048 | MS.gene21644 | 0.804447 | 2.15E-49 | -1.69E-46 |
| MS.gene057076 | MS.gene21644 | 0.813457 | 2.54E-51 | -1.69E-46 |
| MS.gene057146 | MS.gene21644 | 0.824041 | 1.00E-53 | -1.69E-46 |
| MS.gene057416 | MS.gene21644 | 0.845977 | 2.90E-59 | -1.69E-46 |
| MS.gene057658 | MS.gene21644 | 0.80013 | 1.66E-48 | -1.69E-46 |
| MS.gene057831 | MS.gene21644 | 0.806261 | 8.96E-50 | -1.69E-46 |
| MS.gene058007 | MS.gene21644 | 0.804181 | 2.44E-49 | -1.69E-46 |
| MS.gene058019 | MS.gene21644 | 0.818584 | 1.82E-52 | -1.69E-46 |
| MS.gene058376 | MS.gene21644 | 0.807651 | 4.56E-50 | -1.69E-46 |
| MS.gene05837 | MS.gene21644 | 0.817707 | 2.87E-52 | -1.69E-46 |
| MS.gene058419 | MS.gene21644 | 0.823845 | 1.11E-53 | -1.69E-46 |
| MS.gene058544 | MS.gene21644 | 0.803352 | 3.63E-49 | -1.69E-46 |
| MS.gene058700 | MS.gene21644 | 0.811796 | 5.85E-51 | -1.69E-46 |
| MS.gene058838 | MS.gene21644 | 0.805549 | 1.27E-49 | -1.69E-46 |
| MS.gene05883 | MS.gene21644 | 0.81341 | 2.60E-51 | -1.69E-46 |
| MS.gene059023 | MS.gene21644 | 0.811699 | 6.14E-51 | -1.69E-46 |
| MS.gene05934 | MS.gene21644 | 0.844844 | 5.88E-59 | -1.69E-46 |
| MS.gene059455 | MS.gene21644 | 0.811519 | 6.72E-51 | -1.69E-46 |
| MS.gene059677 | MS.gene21644 | 0.808155 | 3.56E-50 | -1.69E-46 |
| MS.gene059718 | MS.gene21644 | 0.809982 | 1.45E-50 | -1.69E-46 |
| MS.gene059902 | MS.gene21644 | 0.806391 | 8.41E-50 | -1.69E-46 |
| MS.gene06077 | MS.gene21644 | 0.81404 | 1.89E-51 | -1.69E-46 |
| MS.gene060835 | MS.gene21644 | 0.803635 | 3.17E-49 | -1.69E-46 |
| MS.gene06092 | MS.gene21644 | 0.829619 | 4.65E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene73340 | MS.gene21644 | PPI |
| MS.gene21644 | MS.gene82807 | PPI |
| MS.gene21644 | MS.gene024472 | PPI |
| MS.gene060400 | MS.gene21644 | PPI |
| MS.gene21644 | MS.gene40102 | PPI |
| MS.gene21644 | MS.gene025937 | PPI |
| MS.gene21644 | MS.gene037013 | PPI |
| MS.gene21644 | MS.gene053627 | PPI |
| MS.gene21644 | MS.gene90270 | PPI |
| MS.gene21644 | MS.gene053078 | PPI |
| MS.gene22823 | MS.gene21644 | PPI |
| MS.gene21644 | MS.gene050620 | PPI |
| MS.gene21644 | MS.gene032655 | PPI |
| MS.gene21644 | MS.gene57695 | PPI |
| MS.gene028526 | MS.gene21644 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21644.t1 | MTR_5g009670 | 99.013 | 304 | 3 | 0 | 1 | 304 | 1 | 304 | 0.0 | 625 |
| MS.gene21644.t1 | MTR_5g009670 | 99.194 | 248 | 2 | 0 | 1 | 248 | 1 | 248 | 0.0 | 511 |
| MS.gene21644.t1 | MTR_4g092780 | 71.803 | 305 | 84 | 2 | 1 | 304 | 1 | 304 | 1.89e-165 | 462 |
| MS.gene21644.t1 | MTR_7g116910 | 69.836 | 305 | 90 | 2 | 1 | 304 | 1 | 304 | 3.15e-165 | 462 |
| MS.gene21644.t1 | MTR_2g036810 | 67.241 | 290 | 93 | 1 | 17 | 304 | 14 | 303 | 1.18e-152 | 430 |
| MS.gene21644.t1 | MTR_4g005740 | 66.667 | 285 | 93 | 1 | 22 | 304 | 15 | 299 | 5.05e-146 | 413 |
| MS.gene21644.t1 | MTR_6g004520 | 54.138 | 290 | 130 | 3 | 15 | 304 | 3 | 289 | 4.01e-115 | 334 |
| MS.gene21644.t1 | MTR_6g004520 | 54.138 | 290 | 130 | 3 | 15 | 304 | 61 | 347 | 9.04e-115 | 335 |
| MS.gene21644.t1 | MTR_6g004520 | 54.138 | 290 | 130 | 3 | 15 | 304 | 50 | 336 | 1.75e-114 | 334 |
| MS.gene21644.t1 | MTR_5g020640 | 33.218 | 289 | 174 | 7 | 15 | 301 | 41 | 312 | 5.82e-43 | 150 |
| MS.gene21644.t1 | MTR_5g020640 | 36.464 | 181 | 107 | 4 | 15 | 195 | 41 | 213 | 4.99e-30 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21644.t1 | AT4G23730 | 69.608 | 306 | 91 | 2 | 1 | 304 | 1 | 306 | 1.13e-165 | 462 |
| MS.gene21644.t1 | AT3G61610 | 74.483 | 290 | 73 | 1 | 16 | 304 | 15 | 304 | 6.52e-165 | 461 |
| MS.gene21644.t1 | AT3G61610 | 74.483 | 290 | 73 | 1 | 16 | 304 | 9 | 298 | 7.22e-165 | 461 |
| MS.gene21644.t1 | AT4G23730 | 68.269 | 312 | 91 | 3 | 1 | 304 | 1 | 312 | 5.78e-163 | 456 |
| MS.gene21644.t1 | AT4G23730 | 73.881 | 268 | 68 | 2 | 39 | 304 | 4 | 271 | 1.60e-154 | 433 |
| MS.gene21644.t1 | AT5G57330 | 67.698 | 291 | 92 | 1 | 16 | 304 | 9 | 299 | 2.79e-154 | 434 |
| MS.gene21644.t1 | AT3G01590 | 60.000 | 285 | 112 | 2 | 20 | 304 | 8 | 290 | 6.11e-130 | 372 |
| MS.gene21644.t1 | AT3G01590 | 60.000 | 285 | 112 | 2 | 20 | 304 | 8 | 290 | 6.11e-130 | 372 |
| MS.gene21644.t1 | AT5G14500 | 57.586 | 290 | 121 | 2 | 15 | 304 | 3 | 290 | 8.30e-130 | 372 |
| MS.gene21644.t1 | AT5G14500 | 53.016 | 315 | 121 | 3 | 15 | 304 | 3 | 315 | 1.36e-123 | 357 |
| MS.gene21644.t1 | AT4G25900 | 51.042 | 288 | 136 | 2 | 16 | 303 | 32 | 314 | 4.60e-112 | 327 |
| MS.gene21644.t1 | AT5G66530 | 34.021 | 291 | 171 | 9 | 13 | 300 | 6 | 278 | 1.37e-44 | 153 |
| MS.gene21644.t1 | AT5G66530 | 34.021 | 291 | 171 | 9 | 13 | 300 | 34 | 306 | 2.97e-44 | 153 |
| MS.gene21644.t1 | AT5G66530 | 34.021 | 291 | 171 | 9 | 13 | 300 | 34 | 306 | 2.97e-44 | 153 |
| MS.gene21644.t1 | AT5G66530 | 34.021 | 291 | 171 | 9 | 13 | 300 | 34 | 306 | 2.97e-44 | 153 |
Find 75 sgRNAs with CRISPR-Local
Find 152 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAAAGTCAATAGTGGATTT+TGG | 0.129984 | 5.2:-3267781 | MS.gene21644:CDS |
| ATTCCTCCTCGTACTGGTTT+TGG | 0.222742 | 5.2:+3269176 | None:intergenic |
| GACAGTGAAGTGAGGGTAGA+AGG | 0.250795 | 5.2:-3268264 | MS.gene21644:intron |
| GATTTGCAAGGAATAAGATA+TGG | 0.355587 | 5.2:-3269006 | MS.gene21644:CDS |
| TTACACATTGAATAGTTGTT+TGG | 0.364398 | 5.2:-3267822 | MS.gene21644:intron |
| ACGGAGGGCAAGTTCTTTCA+TGG | 0.380303 | 5.2:-3269486 | MS.gene21644:CDS |
| AAGGCTTGCCATTGACATTC+CGG | 0.382320 | 5.2:+3268516 | None:intergenic |
| TACGGACCTGATATCTGATA+TGG | 0.394110 | 5.2:+3268460 | None:intergenic |
| TGAAGCGTTCCTTCTGGTAT+AGG | 0.403091 | 5.2:+3268214 | None:intergenic |
| CTGAAGACGATATGAAGATA+TGG | 0.407969 | 5.2:-3268907 | MS.gene21644:CDS |
| CGGCCGGTCCATTCTTCTCC+CGG | 0.408566 | 5.2:+3267691 | None:intergenic |
| GTTCTGTGAAGCGTTCCTTC+TGG | 0.411774 | 5.2:+3268208 | None:intergenic |
| TTATCTCGATTCTAGCAGTA+TGG | 0.412644 | 5.2:-3268037 | MS.gene21644:CDS |
| TGAAGTGAGGGTAGAAGGGT+TGG | 0.418375 | 5.2:-3268259 | MS.gene21644:CDS |
| ATTACCTGTGGAAAACAGAT+TGG | 0.421320 | 5.2:+3269152 | None:intergenic |
| GAAAGACACTTACCAACATC+TGG | 0.431173 | 5.2:+3267958 | None:intergenic |
| ATTTCTCCATATCAGATATC+AGG | 0.439981 | 5.2:-3268466 | MS.gene21644:intron |
| GTTTCGCCTAAGGGTCTGTT+TGG | 0.445507 | 5.2:-3268573 | MS.gene21644:CDS |
| GAATAGTTGTTTGGAATCCC+TGG | 0.459977 | 5.2:-3267813 | MS.gene21644:intron |
| CCATTTGAATCCGTGGGCAG+TGG | 0.460101 | 5.2:+3268962 | None:intergenic |
| AGAAGAATGGACCGGCCGCT+TGG | 0.461091 | 5.2:-3267686 | MS.gene21644:CDS |
| GTTGTTCTTCGAAACGTTCG+AGG | 0.461265 | 5.2:-3269628 | MS.gene21644:CDS |
| AAATCCGATAACCTTAAAAC+CGG | 0.461715 | 5.2:-3267710 | MS.gene21644:CDS |
| CCTTAAAACCGGGAGAAGAA+TGG | 0.474210 | 5.2:-3267699 | MS.gene21644:CDS |
| TTACCTGTGGAAAACAGATT+GGG | 0.476013 | 5.2:+3269153 | None:intergenic |
| ATTGGGATTCCTCCTCGTAC+TGG | 0.478898 | 5.2:+3269170 | None:intergenic |
| AACATCAGTTGAAGTTATCA+AGG | 0.479030 | 5.2:-3269671 | MS.gene21644:CDS |
| GAATCCGTGGGCAGTGGTGG+AGG | 0.481987 | 5.2:+3268968 | None:intergenic |
| TTGAAGTCAAGTCAAGTAGA+AGG | 0.484710 | 5.2:+3267649 | None:intergenic |
| CAGCTTCGAGTTTCGCCTAA+GGG | 0.487911 | 5.2:-3268582 | MS.gene21644:CDS |
| TCAGCTTCGAGTTTCGCCTA+AGG | 0.489943 | 5.2:-3268583 | MS.gene21644:intron |
| ACAGTGAAGTGAGGGTAGAA+GGG | 0.492013 | 5.2:-3268263 | MS.gene21644:intron |
| CCTTCTGGTATAGGTTATCC+AGG | 0.493007 | 5.2:+3268223 | None:intergenic |
| TGCTTTAACATTTGAATCTG+AGG | 0.495159 | 5.2:-3268178 | MS.gene21644:intron |
| GTTATTGTTCACTAGTACCA+AGG | 0.506527 | 5.2:-3269443 | MS.gene21644:intron |
| TGAATATGATATCTCGCATC+CGG | 0.512657 | 5.2:-3268535 | MS.gene21644:CDS |
| ATTTGCAAGGAATAAGATAT+GGG | 0.514716 | 5.2:-3269005 | MS.gene21644:CDS |
| TCTCGCATCCGGAATGTCAA+TGG | 0.518066 | 5.2:-3268524 | MS.gene21644:CDS |
| TTCAGGTTAGCTTGCACGGA+GGG | 0.519016 | 5.2:-3269501 | MS.gene21644:intron |
| TTTGAATCCGTGGGCAGTGG+TGG | 0.520143 | 5.2:+3268965 | None:intergenic |
| AATAGTTGTTTGGAATCCCT+GGG | 0.525696 | 5.2:-3267812 | MS.gene21644:intron |
| CCACTGCCCACGGATTCAAA+TGG | 0.526642 | 5.2:-3268962 | MS.gene21644:CDS |
| CATGTTGCTCTAGTGTTCCA+CGG | 0.532159 | 5.2:+3269029 | None:intergenic |
| TTTCTTCTCATGATCTAGAA+CGG | 0.537918 | 5.2:+3268011 | None:intergenic |
| ATCTTCATATCGTCTTCAGA+TGG | 0.538140 | 5.2:+3268911 | None:intergenic |
| AGGGTCTGTTTGGCAGCAGA+TGG | 0.540406 | 5.2:-3268563 | MS.gene21644:CDS |
| AACAGAAAGCTCCAAGCGGC+CGG | 0.541122 | 5.2:+3267675 | None:intergenic |
| TGCGTGGATGGTGCAGCAGT+TGG | 0.542462 | 5.2:-3267733 | MS.gene21644:CDS |
| GTTGGAGACTCTTGACTACC+TGG | 0.542710 | 5.2:-3268241 | MS.gene21644:CDS |
| AAGCAAATGCTCTGCGTGGA+TGG | 0.557893 | 5.2:-3267745 | MS.gene21644:CDS |
| GAAATATGTATGAAATGCAA+AGG | 0.575295 | 5.2:+3268484 | None:intergenic |
| AAGGAAGGTCTTCCAGATGT+TGG | 0.585878 | 5.2:-3267970 | MS.gene21644:intron |
| CTGCTGCCAAACAGACCCTT+AGG | 0.589405 | 5.2:+3268567 | None:intergenic |
| CGATTATTGACAGTGAAGTG+AGG | 0.590022 | 5.2:-3268272 | MS.gene21644:intron |
| AAATCCTCCACCACTGCCCA+CGG | 0.590093 | 5.2:-3268972 | MS.gene21644:CDS |
| AAGGAACGCTTCACAGAACA+AGG | 0.595906 | 5.2:-3268204 | MS.gene21644:CDS |
| AAACCGGGAGAAGAATGGAC+CGG | 0.609197 | 5.2:-3267694 | MS.gene21644:CDS |
| AATGCAAAGGAGAAACTGAA+AGG | 0.627775 | 5.2:+3268497 | None:intergenic |
| GATTATTGACAGTGAAGTGA+GGG | 0.633522 | 5.2:-3268271 | MS.gene21644:intron |
| ACGAACATTTGTGATACGAA+AGG | 0.635723 | 5.2:-3267989 | MS.gene21644:CDS |
| ATATGAAGATATGGCCACAT+AGG | 0.635993 | 5.2:-3268898 | MS.gene21644:intron |
| CCTGGATAACCTATACCAGA+AGG | 0.640312 | 5.2:-3268223 | MS.gene21644:CDS |
| CGTGGAACACTAGAGCAACA+TGG | 0.643884 | 5.2:-3269028 | MS.gene21644:CDS |
| TAAAGCGCGTCGCACCTATG+TGG | 0.646274 | 5.2:+3268884 | None:intergenic |
| TAGAGCAACATGGATTTGCA+AGG | 0.656362 | 5.2:-3269018 | MS.gene21644:CDS |
| TTTCAGGTTAGCTTGCACGG+AGG | 0.662583 | 5.2:-3269502 | MS.gene21644:intron |
| ACATTTGTGATACGAAAGGA+AGG | 0.663497 | 5.2:-3267985 | MS.gene21644:CDS |
| CGTTCGAGGAGCTTCAGCAA+CGG | 0.664112 | 5.2:-3269614 | MS.gene21644:intron |
| CTTTCATGGAAAACAGAACG+CGG | 0.670837 | 5.2:-3269472 | MS.gene21644:CDS |
| TGCGAGATATCATATTCAGA+CGG | 0.674803 | 5.2:+3268540 | None:intergenic |
| AATCCGATAACCTTAAAACC+GGG | 0.679316 | 5.2:-3267709 | MS.gene21644:CDS |
| GTACAAGCAAATGCTCTGCG+TGG | 0.681422 | 5.2:-3267749 | MS.gene21644:CDS |
| CCTCCAAAACCAGTACGAGG+AGG | 0.735431 | 5.2:-3269179 | MS.gene21644:CDS |
| GTACAACAGAAAGCTCCAAG+CGG | 0.740861 | 5.2:+3267671 | None:intergenic |
| ACTCCTCCAAAACCAGTACG+AGG | 0.754632 | 5.2:-3269182 | MS.gene21644:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATATACTTGTTATTATAT+AGG | + | chr5.2:3268703-3268722 | None:intergenic | 10.0% |
| !!! | AATTTAACTTGATAAATATA+AGG | + | chr5.2:3269246-3269265 | None:intergenic | 10.0% |
| !! | AAAATTTAAAAACGAACAAA+AGG | + | chr5.2:3269022-3269041 | None:intergenic | 15.0% |
| !! | ATATAGATAGATAGATAATA+AGG | - | chr5.2:3268283-3268302 | MS.gene21644:intron | 15.0% |
| !!! | ACATTTTACAAACAAAATTA+AGG | + | chr5.2:3268117-3268136 | None:intergenic | 15.0% |
| !! | CAAAATCTAAGTACTATAAT+TGG | - | chr5.2:3269457-3269476 | MS.gene21644:CDS | 20.0% |
| !! | CAAACAAAATTAAGGTTAAT+TGG | + | chr5.2:3268109-3268128 | None:intergenic | 20.0% |
| !! | GCTATATATAGAACTATTAA+AGG | - | chr5.2:3268589-3268608 | MS.gene21644:intron | 20.0% |
| !! | TATATAGATTTCTGTAATTC+AGG | + | chr5.2:3268268-3268287 | None:intergenic | 20.0% |
| !!! | AGCATTTTACTTATGAATTT+GGG | - | chr5.2:3268533-3268552 | MS.gene21644:CDS | 20.0% |
| !!! | CAAGATCAATTATTTTATCA+AGG | - | chr5.2:3268957-3268976 | MS.gene21644:CDS | 20.0% |
| ! | AATAACTGAGATAAATTGAG+AGG | - | chr5.2:3268567-3268586 | MS.gene21644:CDS | 25.0% |
| ! | AGATAGATAGATAATAAGGT+TGG | - | chr5.2:3268287-3268306 | MS.gene21644:intron | 25.0% |
| ! | ATTCAATGTGTAAATTGACA+TGG | + | chr5.2:3269537-3269556 | None:intergenic | 25.0% |
| ! | ATTTGCAAGGAATAAGATAT+GGG | - | chr5.2:3268361-3268380 | MS.gene21644:intron | 25.0% |
| ! | CAAATTCATAAGTAAAATGC+TGG | + | chr5.2:3268534-3268553 | None:intergenic | 25.0% |
| ! | GAAAAAATCAAAGTCAATAG+TGG | - | chr5.2:3269578-3269597 | MS.gene21644:intron | 25.0% |
| ! | GAAATATGTATGAAATGCAA+AGG | + | chr5.2:3268885-3268904 | None:intergenic | 25.0% |
| ! | TAGAACTATTAAAGGACTAT+TGG | - | chr5.2:3268597-3268616 | MS.gene21644:intron | 25.0% |
| ! | TTACACATTGAATAGTTGTT+TGG | - | chr5.2:3269544-3269563 | MS.gene21644:intron | 25.0% |
| !! | CAGCATTTTACTTATGAATT+TGG | - | chr5.2:3268532-3268551 | MS.gene21644:CDS | 25.0% |
| !! | CATTTTACTTATGAATTTGG+GGG | - | chr5.2:3268535-3268554 | MS.gene21644:CDS | 25.0% |
| !! | GCATTTTACTTATGAATTTG+GGG | - | chr5.2:3268534-3268553 | MS.gene21644:CDS | 25.0% |
| !! | TTGTTATTATATAGGAACTG+GGG | + | chr5.2:3268695-3268714 | None:intergenic | 25.0% |
| !!! | ACTTGTTATTATATAGGAAC+TGG | + | chr5.2:3268697-3268716 | None:intergenic | 25.0% |
| !!! | ATAGTACTTAGATTTTGAGT+CGG | + | chr5.2:3269454-3269473 | None:intergenic | 25.0% |
| !!! | CTTGTTATTATATAGGAACT+GGG | + | chr5.2:3268696-3268715 | None:intergenic | 25.0% |
| !!! | TGGTGTGATTTATTTTAGTT+TGG | - | chr5.2:3268311-3268330 | MS.gene21644:intron | 25.0% |
| AAATCCGATAACCTTAAAAC+CGG | - | chr5.2:3269656-3269675 | MS.gene21644:CDS | 30.0% | |
| AACATCAGTTGAAGTTATCA+AGG | - | chr5.2:3267695-3267714 | MS.gene21644:CDS | 30.0% | |
| AATGTGTAAATTGACATGGA+TGG | + | chr5.2:3269533-3269552 | None:intergenic | 30.0% | |
| AGCACCAATCAAATTCAAAA+GGG | + | chr5.2:3267825-3267844 | None:intergenic | 30.0% | |
| ATCTTGAGAATTGAGATTGT+TGG | - | chr5.2:3268728-3268747 | MS.gene21644:intron | 30.0% | |
| ATTTCTCCATATCAGATATC+AGG | - | chr5.2:3268900-3268919 | MS.gene21644:CDS | 30.0% | |
| ATTTCTCTCTCTTTCCAATT+AGG | - | chr5.2:3268153-3268172 | MS.gene21644:intron | 30.0% | |
| CAAAAAAACCTCATTTACCT+TGG | + | chr5.2:3267943-3267962 | None:intergenic | 30.0% | |
| GAAGTTATCAAGGACAAAAA+TGG | - | chr5.2:3267705-3267724 | MS.gene21644:CDS | 30.0% | |
| GATTTGCAAGGAATAAGATA+TGG | - | chr5.2:3268360-3268379 | MS.gene21644:intron | 30.0% | |
| TAGCACCAATCAAATTCAAA+AGG | + | chr5.2:3267826-3267845 | None:intergenic | 30.0% | |
| TCAATAATCGGCAAAAGTTA+AGG | + | chr5.2:3269084-3269103 | None:intergenic | 30.0% | |
| TGCTTTAACATTTGAATCTG+AGG | - | chr5.2:3269188-3269207 | MS.gene21644:CDS | 30.0% | |
| TTTCTTCTCATGATCTAGAA+CGG | + | chr5.2:3269358-3269377 | None:intergenic | 30.0% | |
| ! | AGATAGATAATAAGGTTGGT+TGG | - | chr5.2:3268291-3268310 | MS.gene21644:intron | 30.0% |
| ! | AGCATGTTGGTAAAATTTCA+AGG | + | chr5.2:3269279-3269298 | None:intergenic | 30.0% |
| ! | CTACTATCTAAAATTACCTG+TGG | + | chr5.2:3268229-3268248 | None:intergenic | 30.0% |
| ! | GAAATTTTACCAACATGCTA+AGG | - | chr5.2:3269280-3269299 | MS.gene21644:intron | 30.0% |
| ! | GCATGTTGGTAAAATTTCAA+GGG | + | chr5.2:3269278-3269297 | None:intergenic | 30.0% |
| ! | TATATGTGATAGTGATTCGT+AGG | - | chr5.2:3268064-3268083 | MS.gene21644:intron | 30.0% |
| ! | TCTACCCTTTTGAATTTGAT+TGG | - | chr5.2:3267818-3267837 | MS.gene21644:intron | 30.0% |
| ! | TGATCTTATCTTGAAAGTGT+TGG | + | chr5.2:3268939-3268958 | None:intergenic | 30.0% |
| !! | GCGATTCATTTTTCTTTGTA+AGG | + | chr5.2:3268632-3268651 | None:intergenic | 30.0% |
| !! | TTATCTTGAAAGTGTTGGAA+TGG | + | chr5.2:3268934-3268953 | None:intergenic | 30.0% |
| !!! | AAAGTCAATAGTGGATTTTG+GGG | - | chr5.2:3269587-3269606 | MS.gene21644:intron | 30.0% |
| !!! | CAAAGTCAATAGTGGATTTT+GGG | - | chr5.2:3269586-3269605 | MS.gene21644:intron | 30.0% |
| !!! | TCAAAGTCAATAGTGGATTT+TGG | - | chr5.2:3269585-3269604 | MS.gene21644:intron | 30.0% |
| !!! | TTATTTTAGTTTGGAAACCG+TGG | - | chr5.2:3268320-3268339 | MS.gene21644:intron | 30.0% |
| AATCCGATAACCTTAAAACC+GGG | - | chr5.2:3269657-3269676 | MS.gene21644:CDS | 35.0% | |
| AATGCAAAGGAGAAACTGAA+AGG | + | chr5.2:3268872-3268891 | None:intergenic | 35.0% | |
| ACATTTGTGATACGAAAGGA+AGG | - | chr5.2:3269381-3269400 | MS.gene21644:intron | 35.0% | |
| ACGAACATTTGTGATACGAA+AGG | - | chr5.2:3269377-3269396 | MS.gene21644:intron | 35.0% | |
| AGTGCTACATAGGAAATAAC+TGG | + | chr5.2:3267968-3267987 | None:intergenic | 35.0% | |
| ATATGAAGATATGGCCACAT+AGG | - | chr5.2:3268468-3268487 | MS.gene21644:CDS | 35.0% | |
| ATCTTCATATCGTCTTCAGA+TGG | + | chr5.2:3268458-3268477 | None:intergenic | 35.0% | |
| ATTACCTGTGGAAAACAGAT+TGG | + | chr5.2:3268217-3268236 | None:intergenic | 35.0% | |
| CAAATAAGACCTTAGCATGT+TGG | + | chr5.2:3269292-3269311 | None:intergenic | 35.0% | |
| CAAATTCAAAAGGGTAGATG+CGG | + | chr5.2:3267816-3267835 | None:intergenic | 35.0% | |
| CTCACTTCACTGTCAATAAT+CGG | + | chr5.2:3269096-3269115 | None:intergenic | 35.0% | |
| CTGAAGACGATATGAAGATA+TGG | - | chr5.2:3268459-3268478 | MS.gene21644:intron | 35.0% | |
| GATTATTGACAGTGAAGTGA+GGG | - | chr5.2:3269095-3269114 | MS.gene21644:intron | 35.0% | |
| GTTATTGTTCACTAGTACCA+AGG | - | chr5.2:3267923-3267942 | MS.gene21644:intron | 35.0% | |
| TGAATATGATATCTCGCATC+CGG | - | chr5.2:3268831-3268850 | MS.gene21644:intron | 35.0% | |
| TGCGAGATATCATATTCAGA+CGG | + | chr5.2:3268829-3268848 | None:intergenic | 35.0% | |
| TTACCTGTGGAAAACAGATT+GGG | + | chr5.2:3268216-3268235 | None:intergenic | 35.0% | |
| TTATCTCGATTCTAGCAGTA+TGG | - | chr5.2:3269329-3269348 | MS.gene21644:intron | 35.0% | |
| ! | AATAGTTGTTTGGAATCCCT+GGG | - | chr5.2:3269554-3269573 | MS.gene21644:intron | 35.0% |
| ! | GTATCTAGCTACATGCTTTT+GGG | + | chr5.2:3268673-3268692 | None:intergenic | 35.0% |
| ! | TAACATTTGAATCTGAGGTG+AGG | - | chr5.2:3269193-3269212 | MS.gene21644:CDS | 35.0% |
| !! | AAGGTCTTATTTGTGTTAGG+TGG | - | chr5.2:3269299-3269318 | MS.gene21644:intron | 35.0% |
| !! | GCTAAGGTCTTATTTGTGTT+AGG | - | chr5.2:3269296-3269315 | MS.gene21644:intron | 35.0% |
| !!! | GACTTTGATTTTTTCTCCCA+GGG | + | chr5.2:3269573-3269592 | None:intergenic | 35.0% |
| !!! | TGACTTTGATTTTTTCTCCC+AGG | + | chr5.2:3269574-3269593 | None:intergenic | 35.0% |
| AAATGAATCGCATCCAAGAG+AGG | - | chr5.2:3268641-3268660 | MS.gene21644:intron | 40.0% | |
| ACACCAATCATAGAAGTGAC+CGG | + | chr5.2:3268014-3268033 | None:intergenic | 40.0% | |
| CACTAGTACCAAGGTAAATG+AGG | - | chr5.2:3267932-3267951 | MS.gene21644:intron | 40.0% | |
| CGATTATTGACAGTGAAGTG+AGG | - | chr5.2:3269094-3269113 | MS.gene21644:intron | 40.0% | |
| CTTTCATGGAAAACAGAACG+CGG | - | chr5.2:3267894-3267913 | MS.gene21644:intron | 40.0% | |
| GAAAGACACTTACCAACATC+TGG | + | chr5.2:3269411-3269430 | None:intergenic | 40.0% | |
| TACGGACCTGATATCTGATA+TGG | + | chr5.2:3268909-3268928 | None:intergenic | 40.0% | |
| TAGAGCAACATGGATTTGCA+AGG | - | chr5.2:3268348-3268367 | MS.gene21644:intron | 40.0% | |
| ! | AATCCCAATCTGTTTTCCAC+AGG | - | chr5.2:3268210-3268229 | MS.gene21644:CDS | 40.0% |
| ! | AGCTTTTCCATTTGAATCCG+TGG | + | chr5.2:3268414-3268433 | None:intergenic | 40.0% |
| ! | CTTCGAATTAAAGGCATGTC+CGG | - | chr5.2:3267992-3268011 | MS.gene21644:CDS | 40.0% |
| ! | GAATAGTTGTTTGGAATCCC+TGG | - | chr5.2:3269553-3269572 | MS.gene21644:intron | 40.0% |
| ! | GCACTGACACTTCGAATTAA+AGG | - | chr5.2:3267983-3268002 | MS.gene21644:CDS | 40.0% |
| ! | GCTTTTCCATTTGAATCCGT+GGG | + | chr5.2:3268413-3268432 | None:intergenic | 40.0% |
| ! | GGTATCTAGCTACATGCTTT+TGG | + | chr5.2:3268674-3268693 | None:intergenic | 40.0% |
| !! | GAAAGTGTTGGAATGGCATA+CGG | + | chr5.2:3268927-3268946 | None:intergenic | 40.0% |
| !! | GTTGTGTGTGTGACTTTTTC+AGG | - | chr5.2:3267848-3267867 | MS.gene21644:intron | 40.0% |
| !!! | AATAGTGGATTTTGGGGATG+AGG | - | chr5.2:3269593-3269612 | MS.gene21644:intron | 40.0% |
| !!! | CTTTTTCAGGTTAGCTTGCA+CGG | - | chr5.2:3267861-3267880 | MS.gene21644:intron | 40.0% |
| !!! | TCTCCCGGTTTTAAGGTTAT+CGG | + | chr5.2:3269663-3269682 | None:intergenic | 40.0% |
| !!! | TGGTTTTGGAGGAGTATAGA+CGG | + | chr5.2:3268179-3268198 | None:intergenic | 40.0% |
| !!! | TTTTGGGATTTCTCCTCTCT+TGG | + | chr5.2:3268657-3268676 | None:intergenic | 40.0% |
| AAGGAACGCTTCACAGAACA+AGG | - | chr5.2:3269162-3269181 | MS.gene21644:CDS | 45.0% | |
| AAGGAAGGTCTTCCAGATGT+TGG | - | chr5.2:3269396-3269415 | MS.gene21644:intron | 45.0% | |
| AAGGCTTGCCATTGACATTC+CGG | + | chr5.2:3268853-3268872 | None:intergenic | 45.0% | |
| ACAGTGAAGTGAGGGTAGAA+GGG | - | chr5.2:3269103-3269122 | MS.gene21644:intron | 45.0% | |
| CATGTTGCTCTAGTGTTCCA+CGG | + | chr5.2:3268340-3268359 | None:intergenic | 45.0% | |
| CCTGGATAACCTATACCAGA+AGG | - | chr5.2:3269143-3269162 | MS.gene21644:intron | 45.0% | |
| CCTTAAAACCGGGAGAAGAA+TGG | - | chr5.2:3269667-3269686 | MS.gene21644:CDS | 45.0% | |
| CCTTCTGGTATAGGTTATCC+AGG | + | chr5.2:3269146-3269165 | None:intergenic | 45.0% | |
| GTACAACAGAAAGCTCCAAG+CGG | + | chr5.2:3269698-3269717 | None:intergenic | 45.0% | |
| GTTGTTCTTCGAAACGTTCG+AGG | - | chr5.2:3267738-3267757 | MS.gene21644:CDS | 45.0% | |
| TGTCCGGTCACTTCTATGAT+TGG | - | chr5.2:3268008-3268027 | MS.gene21644:CDS | 45.0% | |
| ! | AGGAGTATAGACGGCCTAAT+TGG | + | chr5.2:3268170-3268189 | None:intergenic | 45.0% |
| ! | CCATTCTTCTCCCGGTTTTA+AGG | + | chr5.2:3269670-3269689 | None:intergenic | 45.0% |
| ! | TCGAAGTGTCAGTGCTACAT+AGG | + | chr5.2:3267978-3267997 | None:intergenic | 45.0% |
| ! | TGAAGCGTTCCTTCTGGTAT+AGG | + | chr5.2:3269155-3269174 | None:intergenic | 45.0% |
| !! | ATCAGACACAGCACTGATAC+TGG | + | chr5.2:3268045-3268064 | None:intergenic | 45.0% |
| !!! | ATTCCTCCTCGTACTGGTTT+TGG | + | chr5.2:3268193-3268212 | None:intergenic | 45.0% |
| AAACCGGGAGAAGAATGGAC+CGG | - | chr5.2:3269672-3269691 | MS.gene21644:CDS | 50.0% | |
| AAGCAAATGCTCTGCGTGGA+TGG | - | chr5.2:3269621-3269640 | MS.gene21644:CDS | 50.0% | |
| ACGGAGGGCAAGTTCTTTCA+TGG | - | chr5.2:3267880-3267899 | MS.gene21644:intron | 50.0% | |
| ACTCCTCCAAAACCAGTACG+AGG | - | chr5.2:3268184-3268203 | MS.gene21644:CDS | 50.0% | |
| ATTGGGATTCCTCCTCGTAC+TGG | + | chr5.2:3268199-3268218 | None:intergenic | 50.0% | |
| CGTGGAACACTAGAGCAACA+TGG | - | chr5.2:3268338-3268357 | MS.gene21644:intron | 50.0% | |
| GACAGTGAAGTGAGGGTAGA+AGG | - | chr5.2:3269102-3269121 | MS.gene21644:intron | 50.0% | |
| GTACAAGCAAATGCTCTGCG+TGG | - | chr5.2:3269617-3269636 | MS.gene21644:CDS | 50.0% | |
| GTTGGAGACTCTTGACTACC+TGG | - | chr5.2:3269125-3269144 | MS.gene21644:intron | 50.0% | |
| GTTTCGCCTAAGGGTCTGTT+TGG | - | chr5.2:3268793-3268812 | MS.gene21644:intron | 50.0% | |
| TCTCGCATCCGGAATGTCAA+TGG | - | chr5.2:3268842-3268861 | MS.gene21644:intron | 50.0% | |
| TGAAGTGAGGGTAGAAGGGT+TGG | - | chr5.2:3269107-3269126 | MS.gene21644:intron | 50.0% | |
| ! | CAGCTTCGAGTTTCGCCTAA+GGG | - | chr5.2:3268784-3268803 | MS.gene21644:intron | 50.0% |
| ! | GTTCTGTGAAGCGTTCCTTC+TGG | + | chr5.2:3269161-3269180 | None:intergenic | 50.0% |
| ! | TCAGCTTCGAGTTTCGCCTA+AGG | - | chr5.2:3268783-3268802 | MS.gene21644:intron | 50.0% |
| !! | TTCAGGTTAGCTTGCACGGA+GGG | - | chr5.2:3267865-3267884 | MS.gene21644:intron | 50.0% |
| !! | TTTCAGGTTAGCTTGCACGG+AGG | - | chr5.2:3267864-3267883 | MS.gene21644:intron | 50.0% |
| AAATCCTCCACCACTGCCCA+CGG | - | chr5.2:3268394-3268413 | MS.gene21644:intron | 55.0% | |
| AACAGAAAGCTCCAAGCGGC+CGG | + | chr5.2:3269694-3269713 | None:intergenic | 55.0% | |
| AGGGTCTGTTTGGCAGCAGA+TGG | - | chr5.2:3268803-3268822 | MS.gene21644:intron | 55.0% | |
| CCACTGCCCACGGATTCAAA+TGG | - | chr5.2:3268404-3268423 | MS.gene21644:intron | 55.0% | |
| CCATTTGAATCCGTGGGCAG+TGG | + | chr5.2:3268407-3268426 | None:intergenic | 55.0% | |
| CCTCCAAAACCAGTACGAGG+AGG | - | chr5.2:3268187-3268206 | MS.gene21644:CDS | 55.0% | |
| CGTTCGAGGAGCTTCAGCAA+CGG | - | chr5.2:3267752-3267771 | MS.gene21644:CDS | 55.0% | |
| CTGCTGCCAAACAGACCCTT+AGG | + | chr5.2:3268802-3268821 | None:intergenic | 55.0% | |
| TAAAGCGCGTCGCACCTATG+TGG | + | chr5.2:3268485-3268504 | None:intergenic | 55.0% | |
| TTTGAATCCGTGGGCAGTGG+TGG | + | chr5.2:3268404-3268423 | None:intergenic | 55.0% | |
| !!! | CCTCCTCGTACTGGTTTTGG+AGG | + | chr5.2:3268190-3268209 | None:intergenic | 55.0% |
| AGAAGAATGGACCGGCCGCT+TGG | - | chr5.2:3269680-3269699 | MS.gene21644:CDS | 60.0% | |
| TGCGTGGATGGTGCAGCAGT+TGG | - | chr5.2:3269633-3269652 | MS.gene21644:CDS | 60.0% | |
| CGGCCGGTCCATTCTTCTCC+CGG | + | chr5.2:3269678-3269697 | None:intergenic | 65.0% | |
| GAATCCGTGGGCAGTGGTGG+AGG | + | chr5.2:3268401-3268420 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 3267660 | 3269728 | 3267660 | ID=MS.gene21644 |
| chr5.2 | mRNA | 3267660 | 3269728 | 3267660 | ID=MS.gene21644.t1;Parent=MS.gene21644 |
| chr5.2 | exon | 3269615 | 3269728 | 3269615 | ID=MS.gene21644.t1.exon1;Parent=MS.gene21644.t1 |
| chr5.2 | CDS | 3269615 | 3269728 | 3269615 | ID=cds.MS.gene21644.t1;Parent=MS.gene21644.t1 |
| chr5.2 | exon | 3269444 | 3269518 | 3269444 | ID=MS.gene21644.t1.exon2;Parent=MS.gene21644.t1 |
| chr5.2 | CDS | 3269444 | 3269518 | 3269444 | ID=cds.MS.gene21644.t1;Parent=MS.gene21644.t1 |
| chr5.2 | exon | 3269157 | 3269213 | 3269157 | ID=MS.gene21644.t1.exon3;Parent=MS.gene21644.t1 |
| chr5.2 | CDS | 3269157 | 3269213 | 3269157 | ID=cds.MS.gene21644.t1;Parent=MS.gene21644.t1 |
| chr5.2 | exon | 3268899 | 3269059 | 3268899 | ID=MS.gene21644.t1.exon4;Parent=MS.gene21644.t1 |
| chr5.2 | CDS | 3268899 | 3269059 | 3268899 | ID=cds.MS.gene21644.t1;Parent=MS.gene21644.t1 |
| chr5.2 | exon | 3268467 | 3268601 | 3268467 | ID=MS.gene21644.t1.exon5;Parent=MS.gene21644.t1 |
| chr5.2 | CDS | 3268467 | 3268601 | 3268467 | ID=cds.MS.gene21644.t1;Parent=MS.gene21644.t1 |
| chr5.2 | exon | 3268179 | 3268281 | 3268179 | ID=MS.gene21644.t1.exon6;Parent=MS.gene21644.t1 |
| chr5.2 | CDS | 3268179 | 3268281 | 3268179 | ID=cds.MS.gene21644.t1;Parent=MS.gene21644.t1 |
| chr5.2 | exon | 3267971 | 3268070 | 3267971 | ID=MS.gene21644.t1.exon7;Parent=MS.gene21644.t1 |
| chr5.2 | CDS | 3267971 | 3268070 | 3267971 | ID=cds.MS.gene21644.t1;Parent=MS.gene21644.t1 |
| chr5.2 | exon | 3267660 | 3267829 | 3267660 | ID=MS.gene21644.t1.exon8;Parent=MS.gene21644.t1 |
| chr5.2 | CDS | 3267660 | 3267829 | 3267660 | ID=cds.MS.gene21644.t1;Parent=MS.gene21644.t1 |
| Gene Sequence |
| Protein sequence |
