AlfalfaGEDB | MS.gene28854

M. sativa cultivar XinJiangDaYe / MS.gene28854

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28854.t1	XP_003626036.2	97.2	144	3	1	1	144	696	838	1.90E-73	285

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28854.t1	Q9SMX9	52.8	144	47	3	1	144	602	724	4.0e-35	149.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28854.t1	G7KUJ2	97.2	144	3	1	1	144	696	838	1.4e-73	285.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene28854	0.808477	3.04E-50	-1.69E-46
MS.gene049159	MS.gene28854	0.842615	2.32E-58	-1.69E-46
MS.gene049341	MS.gene28854	0.807597	4.68E-50	-1.69E-46
MS.gene049404	MS.gene28854	0.867355	1.45E-65	-1.69E-46
MS.gene049435	MS.gene28854	0.812864	3.42E-51	-1.69E-46
MS.gene049501	MS.gene28854	0.814677	1.36E-51	-1.69E-46
MS.gene049850	MS.gene28854	0.830553	2.75E-55	-1.69E-46
MS.gene049979	MS.gene28854	0.810771	9.76E-51	-1.69E-46
MS.gene050020	MS.gene28854	0.824909	6.25E-54	-1.69E-46
MS.gene05016	MS.gene28854	0.837673	4.52E-57	-1.69E-46
MS.gene050273	MS.gene28854	0.807625	4.61E-50	-1.69E-46
MS.gene050442	MS.gene28854	0.831912	1.28E-55	-1.69E-46
MS.gene050689	MS.gene28854	0.801233	9.92E-49	-1.69E-46
MS.gene050807	MS.gene28854	0.85227	5.15E-61	-1.69E-46
MS.gene050808	MS.gene28854	0.803422	3.51E-49	-1.69E-46
MS.gene050815	MS.gene28854	0.800466	1.42E-48	-1.69E-46
MS.gene051180	MS.gene28854	0.809942	1.47E-50	-1.69E-46
MS.gene051198	MS.gene28854	0.823002	1.75E-53	-1.69E-46
MS.gene051279	MS.gene28854	0.808351	3.23E-50	-1.69E-46
MS.gene051328	MS.gene28854	0.810631	1.05E-50	-1.69E-46
MS.gene051388	MS.gene28854	0.800528	1.38E-48	-1.69E-46
MS.gene05157	MS.gene28854	0.81969	1.02E-52	-1.69E-46
MS.gene051719	MS.gene28854	0.843609	1.26E-58	-1.69E-46
MS.gene051720	MS.gene28854	0.826599	2.48E-54	-1.69E-46
MS.gene051744	MS.gene28854	0.812054	5.14E-51	-1.69E-46
MS.gene051800	MS.gene28854	0.805534	1.27E-49	-1.69E-46
MS.gene052020	MS.gene28854	0.845681	3.49E-59	-1.69E-46
MS.gene052323	MS.gene28854	0.811753	5.98E-51	-1.69E-46
MS.gene052516	MS.gene28854	0.843196	1.63E-58	-1.69E-46
MS.gene052519	MS.gene28854	0.800198	1.61E-48	-1.69E-46
MS.gene052611	MS.gene28854	0.831787	1.37E-55	-1.69E-46
MS.gene052612	MS.gene28854	0.807486	4.94E-50	-1.69E-46
MS.gene052665	MS.gene28854	0.857088	2.07E-62	-1.69E-46
MS.gene052863	MS.gene28854	0.833086	6.52E-56	-1.69E-46
MS.gene052896	MS.gene28854	0.804457	2.14E-49	-1.69E-46
MS.gene053008	MS.gene28854	0.800503	1.40E-48	-1.69E-46
MS.gene053166	MS.gene28854	0.818137	2.29E-52	-1.69E-46
MS.gene053221	MS.gene28854	0.801679	8.04E-49	-1.69E-46
MS.gene053226	MS.gene28854	0.816179	6.32E-52	-1.69E-46
MS.gene053565	MS.gene28854	0.812757	3.61E-51	-1.69E-46
MS.gene053599	MS.gene28854	0.800439	1.44E-48	-1.69E-46
MS.gene053621	MS.gene28854	0.813523	2.45E-51	-1.69E-46
MS.gene054467	MS.gene28854	0.821435	4.04E-53	-1.69E-46
MS.gene05447	MS.gene28854	0.802838	4.64E-49	-1.69E-46
MS.gene054559	MS.gene28854	0.821489	3.93E-53	-1.69E-46
MS.gene054584	MS.gene28854	0.801923	7.16E-49	-1.69E-46
MS.gene054651	MS.gene28854	0.846985	1.54E-59	-1.69E-46
MS.gene054775	MS.gene28854	0.810182	1.31E-50	-1.69E-46
MS.gene055227	MS.gene28854	0.810985	8.78E-51	-1.69E-46
MS.gene055356	MS.gene28854	0.803724	3.04E-49	-1.69E-46
MS.gene055357	MS.gene28854	0.831688	1.45E-55	-1.69E-46
MS.gene055358	MS.gene28854	0.802254	6.12E-49	-1.69E-46
MS.gene055465	MS.gene28854	0.818711	1.70E-52	-1.69E-46
MS.gene055466	MS.gene28854	0.8355	1.62E-56	-1.69E-46
MS.gene055468	MS.gene28854	0.815939	7.15E-52	-1.69E-46
MS.gene055512	MS.gene28854	0.828724	7.67E-55	-1.69E-46
MS.gene055818	MS.gene28854	0.82947	5.06E-55	-1.69E-46
MS.gene055890	MS.gene28854	0.814823	1.27E-51	-1.69E-46
MS.gene055891	MS.gene28854	0.802122	6.52E-49	-1.69E-46
MS.gene055926	MS.gene28854	0.80865	2.79E-50	-1.69E-46
MS.gene05604	MS.gene28854	0.812415	4.29E-51	-1.69E-46
MS.gene056173	MS.gene28854	0.839408	1.61E-57	-1.69E-46
MS.gene056308	MS.gene28854	0.801979	6.97E-49	-1.69E-46
MS.gene056366	MS.gene28854	0.851069	1.13E-60	-1.69E-46
MS.gene056507	MS.gene28854	0.801052	1.08E-48	-1.69E-46
MS.gene056553	MS.gene28854	0.81528	1.00E-51	-1.69E-46
MS.gene056595	MS.gene28854	0.801687	8.01E-49	-1.69E-46
MS.gene056643	MS.gene28854	0.808264	3.37E-50	-1.69E-46
MS.gene057060	MS.gene28854	0.806023	1.01E-49	-1.69E-46
MS.gene057444	MS.gene28854	0.823441	1.38E-53	-1.69E-46
MS.gene057869	MS.gene28854	0.851726	7.35E-61	-1.69E-46
MS.gene057915	MS.gene28854	0.806179	9.33E-50	-1.69E-46
MS.gene058009	MS.gene28854	0.807259	5.52E-50	-1.69E-46
MS.gene058010	MS.gene28854	0.840839	6.83E-58	-1.69E-46
MS.gene058339	MS.gene28854	0.80071	1.27E-48	-1.69E-46
MS.gene058625	MS.gene28854	0.834537	2.83E-56	-1.69E-46
MS.gene058636	MS.gene28854	0.824766	6.76E-54	-1.69E-46
MS.gene058829	MS.gene28854	0.809655	1.70E-50	-1.69E-46
MS.gene058979	MS.gene28854	0.800653	1.30E-48	-1.69E-46
MS.gene058982	MS.gene28854	0.801842	7.44E-49	-1.69E-46
MS.gene059015	MS.gene28854	0.802323	5.93E-49	-1.69E-46
MS.gene059128	MS.gene28854	0.813262	2.80E-51	-1.69E-46
MS.gene059607	MS.gene28854	0.81562	8.42E-52	-1.69E-46
MS.gene059842	MS.gene28854	0.819402	1.18E-52	-1.69E-46
MS.gene059937	MS.gene28854	0.829864	4.06E-55	-1.69E-46
MS.gene060310	MS.gene28854	0.801658	8.12E-49	-1.69E-46
MS.gene060312	MS.gene28854	0.804544	2.05E-49	-1.69E-46
MS.gene060322	MS.gene28854	0.824088	9.76E-54	-1.69E-46
MS.gene06052	MS.gene28854	0.801827	7.49E-49	-1.69E-46
MS.gene060587	MS.gene28854	0.818101	2.34E-52	-1.69E-46
MS.gene060677	MS.gene28854	0.81105	8.50E-51	-1.69E-46
MS.gene060803	MS.gene28854	0.826243	3.02E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28854.t1	MTR_7g110320	97.222	144	3	1	1	144	696	838	2.45e-93	288
MS.gene28854.t1	MTR_7g110320	97.222	144	3	1	1	144	696	838	1.71e-92	288
MS.gene28854.t1	MTR_1g086250	79.861	144	29	0	1	144	505	648	6.02e-75	238
MS.gene28854.t1	MTR_1g086250	79.861	144	29	0	1	144	697	840	7.31e-74	238
MS.gene28854.t1	MTR_2g046550	57.746	142	59	1	1	142	480	620	1.97e-48	166
MS.gene28854.t1	MTR_2g046550	57.746	142	59	1	1	142	715	855	4.49e-48	166
MS.gene28854.t1	MTR_1g035010	33.566	143	77	5	1	142	718	843	2.94e-18	80.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28854.t1	AT2G47070	52.778	144	47	3	1	144	473	595	5.65e-43	150
MS.gene28854.t1	AT2G47070	52.778	144	47	3	1	144	602	724	9.52e-43	150
MS.gene28854.t1	AT3G60030	54.795	146	47	4	1	144	640	768	3.15e-41	146
MS.gene28854.t1	AT1G20980	34.752	141	75	5	3	142	754	878	1.16e-18	82.0
MS.gene28854.t1	AT1G76580	30.556	144	76	5	3	143	746	868	6.85e-16	73.9

Find 0 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CAAAATATTTCAGATAAACT+AGG	-	51384:7903-7922	MS.gene28854:CDS	20.0%
!!!	AGTTTATCTGAAATATTTTG+AGG	+	51384:7903-7922	MS.gene28854:intergenic	20.0%
!	ACAGAAAGCAAATAAAATCT+AGG	-	51384:7634-7653	MS.gene28854:CDS	25.0%
!	ATGGATTTCATTCATGAAAT+GGG	-	51384:7600-7619	MS.gene28854:CDS	25.0%
!	CAGAAAGCAAATAAAATCTA+GGG	-	51384:7635-7654	MS.gene28854:CDS	25.0%
!!!	TAGATTTTATTTGCTTTCTG+TGG	+	51384:7635-7654	MS.gene28854:intergenic	25.0%
	AAAGCAAATAAAATCTAGGG+TGG	-	51384:7638-7657	MS.gene28854:CDS	30.0%
	ATTTCATTCATGAAATGGGT+TGG	-	51384:7604-7623	MS.gene28854:CDS	30.0%
	GGTTCGTTTGAATTCTAGTA+TGG	-	51384:7659-7678	MS.gene28854:CDS	35.0%
	TATCTTGCACTTTCAGAGTT+GGG	-	51384:7813-7832	MS.gene28854:CDS	35.0%
!	TTCCATTAGATCGATTCAAG+TGG	-	51384:7688-7707	MS.gene28854:CDS	35.0%
	AGCCACTTGAATCGATCTAA+TGG	+	51384:7693-7712	MS.gene28854:intergenic	40.0%
	CTATTGAACCTCATGCTTGA+TGG	-	51384:7759-7778	MS.gene28854:CDS	40.0%
	GTATCTTGCACTTTCAGAGT+TGG	-	51384:7812-7831	MS.gene28854:CDS	40.0%
	TAAACTAGGACCTGAAGACA+AGG	-	51384:7917-7936	MS.gene28854:CDS	40.0%
	TTGTTTAGACCTGATGCTGT+CGG	-	51384:7969-7988	MS.gene28854:CDS	40.0%
!	ACTCTGAAAGTGCAAGATAC+AGG	+	51384:7812-7831	MS.gene28854:intergenic	40.0%
!	CTCTGAAAGTGCAAGATACA+GGG	+	51384:7811-7830	MS.gene28854:intergenic	40.0%
	AGTTCTCTGTGGACCATGAT+TGG	-	51384:7718-7737	MS.gene28854:CDS	45.0%
	CTTGATGGAACAGTGAGTAC+AGG	-	51384:7774-7793	MS.gene28854:CDS	45.0%
	TAACTACCGCACACCAATCA+TGG	+	51384:7734-7753	MS.gene28854:intergenic	45.0%
	TCACTGTTCCATCAAGCATG+AGG	+	51384:7770-7789	MS.gene28854:intergenic	45.0%
	TTAACCAGTGCCTTGTCTTC+AGG	+	51384:7930-7949	MS.gene28854:intergenic	45.0%
!!	GAAGACAAGGCACTGGTTAA+TGG	-	51384:7930-7949	MS.gene28854:CDS	45.0%
	ACACCACTCCATATAGCAGC+CGG	-	51384:8005-8024	MS.gene28854:CDS	50.0%
	CCATATAGCAGCCGGAAAGA+TGG	-	51384:8013-8032	MS.gene28854:CDS	50.0%
	CCATCTTTCCGGCTGCTATA+TGG	+	51384:8016-8035	MS.gene28854:intergenic	50.0%
	GAGAAATAGCAGGCAGTTGG+TGG	-	51384:7857-7876	MS.gene28854:CDS	50.0%
	GAGCTGTGAGGAGAAATAGC+AGG	-	51384:7847-7866	MS.gene28854:CDS	50.0%
	GAGGAGAAATAGCAGGCAGT+TGG	-	51384:7854-7873	MS.gene28854:CDS	50.0%
	GTGCAAGATACAGGGATGTG+TGG	+	51384:7803-7822	MS.gene28854:intergenic	50.0%
	TGTGGACCATGATTGGTGTG+CGG	-	51384:7725-7744	MS.gene28854:CDS	50.0%
	TTCTCCTCACAGCTCTGTGA+AGG	+	51384:7842-7861	MS.gene28854:intergenic	50.0%
	TTTCCGGCTGCTATATGGAG+TGG	+	51384:8011-8030	MS.gene28854:intergenic	50.0%
!	AGATCGATTCAAGTGGCTCG+TGG	-	51384:7695-7714	MS.gene28854:CDS	50.0%
	AGGACCTGAAGACAAGGCAC+TGG	-	51384:7923-7942	MS.gene28854:CDS	55.0%
	GTCTCCTTCACAGAGCTGTG+AGG	-	51384:7835-7854	MS.gene28854:CDS	55.0%
!	CAGCCGGAAAGATGGTTCTG+AGG	-	51384:8021-8040	MS.gene28854:CDS	55.0%
!	TGGAGTGGTGTCAAACCAGC+AGG	+	51384:7996-8015	MS.gene28854:intergenic	55.0%
	GTGGCTCGTGGAGTTCTCTG+TGG	-	51384:7707-7726	MS.gene28854:CDS	60.0%
	CCAGCAGGACCGACAGCATC+AGG	+	51384:7981-8000	MS.gene28854:intergenic	65.0%
	CCTGATGCTGTCGGTCCTGC+TGG	-	51384:7978-7997	MS.gene28854:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
51384	gene	7600	8045	7600	ID=MS.gene28854
51384	mRNA	7600	8045	7600	ID=MS.gene28854.t1;Parent=MS.gene28854
51384	exon	7600	8045	7600	ID=MS.gene28854.t1.exon1;Parent=MS.gene28854.t1
51384	CDS	7600	8045	7600	ID=cds.MS.gene28854.t1;Parent=MS.gene28854.t1

Gene Sequence

>MS.gene28854

ATGGATTTCATTCATGAAATGGGTTGGCTTCTCCACAGAAAGCAAATAAAATCTAGGGTGGTTCGTTTGAATTCTAGTATGGATCTGTTTCCATTAGATCGATTCAAGTGGCTCGTGGAGTTCTCTGTGGACCATGATTGGTGTGCGGTAGTTAAAAAGCTATTGAACCTCATGCTTGATGGAACAGTGAGTACAGGAGACCACACATCCCTGTATCTTGCACTTTCAGAGTTGGGTCTCCTTCACAGAGCTGTGAGGAGAAATAGCAGGCAGTTGGTGGAGCTTCTTTTGAGATTTGTTCCTCAAAATATTTCAGATAAACTAGGACCTGAAGACAAGGCACTGGTTAATGGAGAGAATCAAAACTTCTTGTTTAGACCTGATGCTGTCGGTCCTGCTGGTTTGACACCACTCCATATAGCAGCCGGAAAGATGGTTCTGAGGAT

Protein sequence

>MS.gene28854.t1

MDFIHEMGWLLHRKQIKSRVVRLNSSMDLFPLDRFKWLVEFSVDHDWCAVVKKLLNLMLDGTVSTGDHTSLYLALSELGLLHRAVRRNSRQLVELLLRFVPQNISDKLGPEDKALVNGENQNFLFRPDAVGPAGLTPLHIAAGKMVLR