AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38274


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38274.t1 MTR_5g096150 98.195 277 5 0 1 277 43 319 0.0 569
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38274.t1 AT4G37000 53.571 280 125 2 1 277 41 318 3.63e-102 301

Find 50 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTACGACCTTGCTTCTTC+TGG 0.137652 5.3:+77762510 None:intergenic
CTCGAAAAGACCTTGTTCTT+TGG 0.238806 5.3:-77761172 MS.gene38274:CDS
CAAAGCAAGTGTTGGGGATA+TGG 0.247429 5.3:-77760938 MS.gene38274:CDS
ATCAGCTCGAACACAAAATT+TGG 0.277458 5.3:+77761239 None:intergenic
CCCAACACTTGCTTTGAAAT+CGG 0.292400 5.3:+77760945 None:intergenic
AGAACAAGAATTAGCATTGT+TGG 0.348949 5.3:+77761206 None:intergenic
TCAAGCTTTCCAAGGTTGTT+TGG 0.358482 5.3:-77760807 MS.gene38274:CDS
AACAGTAAAATACTCCTTGA+TGG 0.363131 5.3:+77760755 None:intergenic
GCTACGACCTTGCTTCTTCT+GGG 0.370091 5.3:+77762511 None:intergenic
AAGACTATTGAGGTTGATCT+TGG 0.379733 5.3:-77760831 MS.gene38274:CDS
AGAAGCTGCAAAACAGATAT+TGG 0.387915 5.3:-77760781 MS.gene38274:CDS
CTCTTATAGTATTGAACATC+AGG 0.390571 5.3:+77762377 None:intergenic
TCCTACTTCTCTCTCGGAAC+AGG 0.405515 5.3:+77760902 None:intergenic
CCGATTTCAAAGCAAGTGTT+GGG 0.413297 5.3:-77760945 MS.gene38274:CDS
CCAACACTTGCTTTGAAATC+GGG 0.426823 5.3:+77760946 None:intergenic
TTAGCATTGTTGGACTGCTT+CGG 0.427662 5.3:+77761216 None:intergenic
CCCGATTTCAAAGCAAGTGT+TGG 0.432798 5.3:-77760946 MS.gene38274:CDS
CGATACACATAGGCAGGCTT+TGG 0.432959 5.3:-77761111 MS.gene38274:CDS
GTGACATAAGGTTGAACCTC+TGG 0.461727 5.3:+77761080 None:intergenic
ATCATTTGAACTGTTAAGAT+AGG 0.468826 5.3:+77761265 None:intergenic
CAATACTATAAGAGCAAGAA+TGG 0.480003 5.3:-77762368 MS.gene38274:CDS
ATCAAGCATGAGATTCTTGT+GGG 0.484852 5.3:+77762457 None:intergenic
TTCCTCTCCTACTTCTCTCT+CGG 0.487400 5.3:+77760896 None:intergenic
ACAGATATTGGAAGCCATCA+AGG 0.487475 5.3:-77760769 MS.gene38274:CDS
ATCTTGGTTCAAGCTTTCCA+AGG 0.509846 5.3:-77760815 MS.gene38274:CDS
GTGCATGCTGAGCTACCAAC+AGG 0.516588 5.3:-77761317 MS.gene38274:CDS
TTGTGGGGTGCTGAAACAAA+TGG 0.519655 5.3:+77762473 None:intergenic
ATATCTAGTGATCCTCCTGT+TGG 0.539776 5.3:+77761302 None:intergenic
CATTAGAAACAAGACTATTG+AGG 0.540436 5.3:-77760841 MS.gene38274:CDS
ACAGGGGAATCAGTGTTGCT+TGG 0.543335 5.3:+77762317 None:intergenic
TAAACTCGATACACATAGGC+AGG 0.546034 5.3:-77761117 MS.gene38274:CDS
ATTCAGACAGAAAATGATGA+AGG 0.546561 5.3:-77761005 MS.gene38274:CDS
AAAAGCGAAGAAGTGACATA+AGG 0.548015 5.3:+77761068 None:intergenic
GCTTCTGGACCAAACAACCT+TGG 0.551283 5.3:+77760798 None:intergenic
GCAAGTGTTGGGGATATGGT+TGG 0.554687 5.3:-77760934 MS.gene38274:CDS
ATGCGAACAAATATAGCGGT+TGG 0.559048 5.3:+77761026 None:intergenic
ACATCAGGTGGAAGTGAACA+AGG 0.566315 5.3:+77762392 None:intergenic
GATCAAGCATGAGATTCTTG+TGG 0.577920 5.3:+77762456 None:intergenic
GCCTGTTCCGAGAGAGAAGT+AGG 0.581979 5.3:-77760903 MS.gene38274:CDS
GGTAATCTGGCCAAAGAACA+AGG 0.591389 5.3:+77761162 None:intergenic
TTCCGAGAGAGAAGTAGGAG+AGG 0.601529 5.3:-77760898 MS.gene38274:CDS
TTATAGTATTGAACATCAGG+TGG 0.606078 5.3:+77762380 None:intergenic
ATGGATACCCAGAAGAAGCA+AGG 0.631772 5.3:-77762518 MS.gene38274:CDS
CGATTTCAAAGCAAGTGTTG+GGG 0.632172 5.3:-77760944 MS.gene38274:CDS
ACACTAAACTCGATACACAT+AGG 0.635620 5.3:-77761121 MS.gene38274:CDS
CATGCTGAGCTACCAACAGG+AGG 0.671949 5.3:-77761314 MS.gene38274:CDS
CTGAATGCGAACAAATATAG+CGG 0.692980 5.3:+77761022 None:intergenic
TCAAGCATGAGATTCTTGTG+GGG 0.732179 5.3:+77762458 None:intergenic
TTACCTTAAAACTTTCTACG+AGG 0.733422 5.3:-77761144 MS.gene38274:CDS
CGGTTGGAGATGCAACACTG+CGG 0.782235 5.3:+77761042 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTGTTATGTTATTTTATT+AGG - chr5.3:77761058-77761077 MS.gene38274:CDS 10.0%
!! ATTATGTTTCTTCAACATAA+GGG + chr5.3:77761581-77761600 None:intergenic 20.0%
!! TATACTCTAACATGAAAATA+TGG + chr5.3:77761010-77761029 None:intergenic 20.0%
!! TCATATAACAAAACAATCAA+TGG + chr5.3:77761794-77761813 None:intergenic 20.0%
!! TTCATGTTAGAGTATATATT+TGG - chr5.3:77761014-77761033 MS.gene38274:CDS 20.0%
!! TTTAAAAGCAAAAAGTAGAA+AGG + chr5.3:77760984-77761003 None:intergenic 20.0%
! AAAAGTAGAAAGGAAATTAC+AGG + chr5.3:77760974-77760993 None:intergenic 25.0%
! AAAGTAGAAAGGAAATTACA+GGG + chr5.3:77760973-77760992 None:intergenic 25.0%
! ATCATTTGAACTGTTAAGAT+AGG + chr5.3:77762008-77762027 None:intergenic 25.0%
! ATGCAAATAACCAAATTAAG+TGG + chr5.3:77761230-77761249 None:intergenic 25.0%
! CATTATGTTTCTTCAACATA+AGG + chr5.3:77761582-77761601 None:intergenic 25.0%
! CTATCTAACTATCTACAAAT+AGG - chr5.3:77761505-77761524 MS.gene38274:intron 25.0%
! TATATATTTGGTTGAGAACT+AGG - chr5.3:77761026-77761045 MS.gene38274:CDS 25.0%
! TTCCTTAATTGTGATTGTTT+GGG - chr5.3:77761422-77761441 MS.gene38274:intron 25.0%
! TTTAGAGAACTGAATCAAAT+TGG - chr5.3:77761314-77761333 MS.gene38274:CDS 25.0%
!! TCTCAGATAGATTTTGTAAT+GGG - chr5.3:77761923-77761942 MS.gene38274:intron 25.0%
!!! TAGAAAGTTTTAAGGTAATC+TGG + chr5.3:77762124-77762143 None:intergenic 25.0%
!!! TATTTTTCCATTAGTTCTCT+AGG - chr5.3:77761721-77761740 MS.gene38274:intron 25.0%
AACAGTAAAATACTCCTTGA+TGG + chr5.3:77762518-77762537 None:intergenic 30.0%
AAGTAGAAAGGAAATTACAG+GGG + chr5.3:77760972-77760991 None:intergenic 30.0%
AGAACAAGAATTAGCATTGT+TGG + chr5.3:77762067-77762086 None:intergenic 30.0%
ATTCAGACAGAAAATGATGA+AGG - chr5.3:77762265-77762284 MS.gene38274:intron 30.0%
CAATACTATAAGAGCAAGAA+TGG - chr5.3:77760902-77760921 MS.gene38274:CDS 30.0%
CATTAGAAACAAGACTATTG+AGG - chr5.3:77762429-77762448 MS.gene38274:CDS 30.0%
GAAGAAACATAATGTTCATG+AGG - chr5.3:77761588-77761607 MS.gene38274:intron 30.0%
GCTTATCTGATAAAAAGAGA+TGG - chr5.3:77762403-77762422 MS.gene38274:CDS 30.0%
GTGATTTCAACCACTTAATT+TGG - chr5.3:77761217-77761236 MS.gene38274:CDS 30.0%
GTTCCTTAATTGTGATTGTT+TGG - chr5.3:77761421-77761440 MS.gene38274:intron 30.0%
TACCCAAACAATCACAATTA+AGG + chr5.3:77761427-77761446 None:intergenic 30.0%
TCAAATAAGCTAATCCAAAC+AGG + chr5.3:77761614-77761633 None:intergenic 30.0%
TTACCTTAAAACTTTCTACG+AGG - chr5.3:77762126-77762145 MS.gene38274:intron 30.0%
! CTCTTATAGTATTGAACATC+AGG + chr5.3:77760896-77760915 None:intergenic 30.0%
! GTCTCAGATAGATTTTGTAA+TGG - chr5.3:77761922-77761941 MS.gene38274:intron 30.0%
! TTATAGTATTGAACATCAGG+TGG + chr5.3:77760893-77760912 None:intergenic 30.0%
AAAAGCGAAGAAGTGACATA+AGG + chr5.3:77762205-77762224 None:intergenic 35.0%
AAGCTATCCTAGAGAACTAA+TGG + chr5.3:77761731-77761750 None:intergenic 35.0%
ACACTAAACTCGATACACAT+AGG - chr5.3:77762149-77762168 MS.gene38274:intron 35.0%
AGAAGCTGCAAAACAGATAT+TGG - chr5.3:77762489-77762508 MS.gene38274:CDS 35.0%
ATCAAGCATGAGATTCTTGT+GGG + chr5.3:77760816-77760835 None:intergenic 35.0%
ATCAGCTCGAACACAAAATT+TGG + chr5.3:77762034-77762053 None:intergenic 35.0%
CAAGTTGTTTATCCAACACA+TGG - chr5.3:77761841-77761860 MS.gene38274:intron 35.0%
CATGTGTTGGATAAACAACT+TGG + chr5.3:77761843-77761862 None:intergenic 35.0%
CTGAATGCGAACAAATATAG+CGG + chr5.3:77762251-77762270 None:intergenic 35.0%
GCTTATTTGAGCTTACATAC+TGG - chr5.3:77761624-77761643 MS.gene38274:intron 35.0%
TTACTAACTACTCACCTTAG+AGG + chr5.3:77761177-77761196 None:intergenic 35.0%
! AAGACTATTGAGGTTGATCT+TGG - chr5.3:77762439-77762458 MS.gene38274:CDS 35.0%
! AATATCTGTTTTGCAGCTTC+TGG + chr5.3:77762490-77762509 None:intergenic 35.0%
! TAGAGTGTCTAAAGTGAGTT+TGG + chr5.3:77761140-77761159 None:intergenic 35.0%
! TGTCCTCGTAGAAAGTTTTA+AGG + chr5.3:77762132-77762151 None:intergenic 35.0%
!! AGATTTTGTAATGGGAAGCT+GGG - chr5.3:77761931-77761950 MS.gene38274:intron 35.0%
!! TAGATTTTGTAATGGGAAGC+TGG - chr5.3:77761930-77761949 MS.gene38274:intron 35.0%
ACAGATATTGGAAGCCATCA+AGG - chr5.3:77762501-77762520 MS.gene38274:CDS 40.0%
ACCATAACTAGACCATGTGT+TGG + chr5.3:77761856-77761875 None:intergenic 40.0%
ATATCTAGTGATCCTCCTGT+TGG + chr5.3:77761971-77761990 None:intergenic 40.0%
ATCTACCAACCAGTGAATCA+AGG + chr5.3:77761395-77761414 None:intergenic 40.0%
ATCTTGGTTCAAGCTTTCCA+AGG - chr5.3:77762455-77762474 MS.gene38274:CDS 40.0%
ATGCGAACAAATATAGCGGT+TGG + chr5.3:77762247-77762266 None:intergenic 40.0%
CCGATTTCAAAGCAAGTGTT+GGG - chr5.3:77762325-77762344 MS.gene38274:CDS 40.0%
CTCGAAAAGACCTTGTTCTT+TGG - chr5.3:77762098-77762117 MS.gene38274:intron 40.0%
GATCAAGCATGAGATTCTTG+TGG + chr5.3:77760817-77760836 None:intergenic 40.0%
TAAACTCGATACACATAGGC+AGG - chr5.3:77762153-77762172 MS.gene38274:intron 40.0%
TAATGTTCATGAGGCCTGTT+TGG - chr5.3:77761597-77761616 MS.gene38274:intron 40.0%
TCAACCATGTTTCTCCTCTA+AGG - chr5.3:77761160-77761179 MS.gene38274:CDS 40.0%
TCAAGCATGAGATTCTTGTG+GGG + chr5.3:77760815-77760834 None:intergenic 40.0%
TCAAGCTTTCCAAGGTTGTT+TGG - chr5.3:77762463-77762482 MS.gene38274:CDS 40.0%
TCCAACACATGGTCTAGTTA+TGG - chr5.3:77761852-77761871 MS.gene38274:intron 40.0%
TTAGCATTGTTGGACTGCTT+CGG + chr5.3:77762057-77762076 None:intergenic 40.0%
! CAAAGAACAAGGTCTTTTCG+AGG + chr5.3:77762100-77762119 None:intergenic 40.0%
! CGATTTCAAAGCAAGTGTTG+GGG - chr5.3:77762326-77762345 MS.gene38274:CDS 40.0%
! GCAAGGTCGTAGCAAATTTT+TGG - chr5.3:77760769-77760788 MS.gene38274:CDS 40.0%
!! CCAACACTTGCTTTGAAATC+GGG + chr5.3:77762327-77762346 None:intergenic 40.0%
!! CCCAACACTTGCTTTGAAAT+CGG + chr5.3:77762328-77762347 None:intergenic 40.0%
!! GGCTTTGGAAAAAATTCCAG+AGG - chr5.3:77762174-77762193 MS.gene38274:intron 40.0%
ACATCAGGTGGAAGTGAACA+AGG + chr5.3:77760881-77760900 None:intergenic 45.0%
ATGGATACCCAGAAGAAGCA+AGG - chr5.3:77760752-77760771 MS.gene38274:CDS 45.0%
CAACCAGTGAATCAAGGCTT+AGG + chr5.3:77761389-77761408 None:intergenic 45.0%
CCCGATTTCAAAGCAAGTGT+TGG - chr5.3:77762324-77762343 MS.gene38274:CDS 45.0%
CTAAGCCTTGATTCACTGGT+TGG - chr5.3:77761387-77761406 MS.gene38274:intron 45.0%
CTCACCTTAGAGGAGAAACA+TGG + chr5.3:77761167-77761186 None:intergenic 45.0%
GGTAATCTGGCCAAAGAACA+AGG + chr5.3:77762111-77762130 None:intergenic 45.0%
GTGACATAAGGTTGAACCTC+TGG + chr5.3:77762193-77762212 None:intergenic 45.0%
TAGCCTAAGCCTTGATTCAC+TGG - chr5.3:77761383-77761402 MS.gene38274:intron 45.0%
TTCCTCTCCTACTTCTCTCT+CGG + chr5.3:77762377-77762396 None:intergenic 45.0%
!! AGAACAAGGTCTTTTCGAGG+TGG + chr5.3:77762097-77762116 None:intergenic 45.0%
!! CAAAGCAAGTGTTGGGGATA+TGG - chr5.3:77762332-77762351 MS.gene38274:CDS 45.0%
!! TTGTGGGGTGCTGAAACAAA+TGG + chr5.3:77760800-77760819 None:intergenic 45.0%
!!! ATTATTTTAATTACTTATTA+AGG - chr5.3:77761467-77761486 MS.gene38274:intron 5.0%
CGATACACATAGGCAGGCTT+TGG - chr5.3:77762159-77762178 MS.gene38274:intron 50.0%
GCTACGACCTTGCTTCTTCT+GGG + chr5.3:77760762-77760781 None:intergenic 50.0%
GCTTCTGGACCAAACAACCT+TGG + chr5.3:77762475-77762494 None:intergenic 50.0%
TCCTACTTCTCTCTCGGAAC+AGG + chr5.3:77762371-77762390 None:intergenic 50.0%
TGCTACGACCTTGCTTCTTC+TGG + chr5.3:77760763-77760782 None:intergenic 50.0%
TTCCGAGAGAGAAGTAGGAG+AGG - chr5.3:77762372-77762391 MS.gene38274:CDS 50.0%
! ACAGGGGAATCAGTGTTGCT+TGG + chr5.3:77760956-77760975 None:intergenic 50.0%
!! GCAAGTGTTGGGGATATGGT+TGG - chr5.3:77762336-77762355 MS.gene38274:CDS 50.0%
CATGCTGAGCTACCAACAGG+AGG - chr5.3:77761956-77761975 MS.gene38274:intron 55.0%
CGGTTGGAGATGCAACACTG+CGG + chr5.3:77762231-77762250 None:intergenic 55.0%
GCCTGTTCCGAGAGAGAAGT+AGG - chr5.3:77762367-77762386 MS.gene38274:CDS 55.0%
GTGCATGCTGAGCTACCAAC+AGG - chr5.3:77761953-77761972 MS.gene38274:intron 55.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 77760752 77762540 77760752 ID=MS.gene38274
chr5.3 mRNA 77760752 77762540 77760752 ID=MS.gene38274.t1;Parent=MS.gene38274
chr5.3 exon 77762319 77762540 77762319 ID=MS.gene38274.t1.exon1;Parent=MS.gene38274.t1
chr5.3 CDS 77762319 77762540 77762319 ID=cds.MS.gene38274.t1;Parent=MS.gene38274.t1
chr5.3 exon 77760752 77761363 77760752 ID=MS.gene38274.t1.exon2;Parent=MS.gene38274.t1
chr5.3 CDS 77760752 77761363 77760752 ID=cds.MS.gene38274.t1;Parent=MS.gene38274.t1
Gene Sequence

>MS.gene38274

ATGGATACCCAGAAGAAGCAAGGTCGTAGCAAATTTTTGGAATTTCCATTTGTTTCAGCACCCCACAAGAATCTCATGCTTGATCTTGTTTCAACAGTTGAAGATCGCTTTGAATCTCATCTTCTTCCTTGTTCACTTCCACCTGATGTTCAATACTATAAGAGCAAGAATGGTACTTCTCAAGTTTCATTGCATATTAGACCAAGCAACACTGATTCCCCTGTAATTTCCTTTCTACTTTTTGCTTTTAAAATTCCATATTTTCATGTTAGAGTATATATTTGGTTGAGAACTAGGTACTTAATTTATTGTTATGTTATTTTATTAGGTTAAGTACACTAAATATTTATCATAGATTATGTTAATGTTTTTAATAAATATTTATCCAAACTCACTTTAGACACTCTATCAACCATGTTTCTCCTCTAAGGTGAGTAGTTAGTAAGATTAGAAAAATTAAGAATTGTGATTTCAACCACTTAATTTGGTTATTTGCATAATACATTAGTTTGTTGTTAATAGTTGATATTAGTAACTTTACTCTCTAAAATTAGTTGCTTACTTTAGAGAACTGAATCAAATTGGCAATGTAGATTTTTATTTTCATTTGAATAATTTTCATTTAATATTGTAGCCTAAGCCTTGATTCACTGGTTGGTAGATGTAATAGTTCCTTAATTGTGATTGTTTGGGTATTTTTTTGCATGTTGTTGAGATTATTTTAATTACTTATTAAGGAAATCATTAACTTATCTATCTAACTATCTACAAATAGGCACTGCAGATAGTTATTTTCTTCATAAATGATTTGTTATTTCAGTGAACTCCCTTATGTTGAAGAAACATAATGTTCATGAGGCCTGTTTGGATTAGCTTATTTGAGCTTACATACTGGCATAAGCACTTATGAGACTGTTTGATAGAACTTCTGAAAACAACTTATGACTTGTATATAGCTTGTTTTCAGCTTATTTTTCCATTAGTTCTCTAGGATAGCTTTATGAAAACAGCTCATAGCTTATATAAAAATATTTTGACTCCATTGATTGTTTTGTTATATGATAAGTGCTTATGTTATAAGCGTTTAACCAAGTTGTTTATCCAACACATGGTCTAGTTATGGTTTTATTCTGAATTTGTTGTTCACTGTTCATTTTTTTGTTGTTGTATGTCTCAGATAGATTTTGTAATGGGAAGCTGGGTGCATGCTGAGCTACCAACAGGAGGATCACTAGATATAACATCTCTTTCAGCCTATCTTAACAGTTCAAATGATGCACCAAATTTTGTGTTCGAGCTGATCCGAAGCAGTCCAACAATGCTAATTCTTGTTCTTGATTTGCCACCTCGAAAAGACCTTGTTCTTTGGCCAGATTACCTTAAAACTTTCTACGAGGACACTAAACTCGATACACATAGGCAGGCTTTGGAAAAAATTCCAGAGGTTCAACCTTATGTCACTTCTTCGCTTTTCATCCGCAGTGTTGCATCTCCAACCGCTATATTTGTTCGCATTCAGACAGAAAATGATGAAGGAGAGCGAATAGACGAGATCATCAAGAACAATATTGACCCGATTTCAAAGCAAGTGTTGGGGATATGGTTGGATCATTGCGCCTGTTCCGAGAGAGAAGTAGGAGAGGAAGATAAAGCTTATCTGATAAAAAGAGATGGTGTCATTAGAAACAAGACTATTGAGGTTGATCTTGGTTCAAGCTTTCCAAGGTTGTTTGGTCCAGAAGCTGCAAAACAGATATTGGAAGCCATCAAGGAGTATTTTACTGTTTGA

Protein sequence

>MS.gene38274.t1

MDTQKKQGRSKFLEFPFVSAPHKNLMLDLVSTVEDRFESHLLPCSLPPDVQYYKSKNGTSQVSLHIRPSNTDSPIDFVMGSWVHAELPTGGSLDITSLSAYLNSSNDAPNFVFELIRSSPTMLILVLDLPPRKDLVLWPDYLKTFYEDTKLDTHRQALEKIPEVQPYVTSSLFIRSVASPTAIFVRIQTENDEGERIDEIIKNNIDPISKQVLGIWLDHCACSEREVGEEDKAYLIKRDGVIRNKTIEVDLGSSFPRLFGPEAAKQILEAIKEYFTV