AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene42162


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42162.t1 MTR_7g075370 76.000 250 39 1 30 279 128 356 1.62e-124 359
MS.gene42162.t1 MTR_7g075470 73.600 250 24 1 30 279 104 311 2.35e-118 342
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 61 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAATCAAACGATGGAAAT+AGG 0.270784 7.3:-30387588 None:intergenic
GTGCAGGAGGTGAAGGAGTC+TGG 0.303169 7.3:+30386902 MS.gene42162:intron
GCAATCTGCTGCGAAGGATA+TGG 0.335315 7.3:+30387363 MS.gene42162:CDS
GAAATCTGCTGCGAAGGATA+TGG 0.341602 7.3:+30387237 MS.gene42162:CDS
GCAGTCTGCTGCGAAGGATA+TGG 0.355180 7.3:+30387300 MS.gene42162:CDS
GCAATCCGCAGCGAAGGATA+TGG 0.359699 7.3:+30387489 MS.gene42162:CDS
AGCCTTCGCTCTGCTTTACT+TGG 0.361216 7.3:+30386959 MS.gene42162:CDS
ATTTCCATCGTTTGATTTGT+CGG 0.381246 7.3:+30387591 MS.gene42162:CDS
TTTACTTGGGAAGGTGATTA+CGG 0.400782 7.3:+30386973 MS.gene42162:CDS
TAGGATGTGAACTTGTTCTA+TGG 0.402680 7.3:-30387569 None:intergenic
GCAATCCGCAACAAAAGATA+TGG 0.403051 7.3:+30387426 MS.gene42162:CDS
CATATCTCCAAGAGTATCAT+TGG 0.418313 7.3:-30387074 None:intergenic
AAAATAGCCAATGATACTCT+TGG 0.432979 7.3:+30387067 MS.gene42162:CDS
GTCCCAGTACAGCTCTGAAT+TGG 0.440833 7.3:-30386997 None:intergenic
ATTGAAGGTAAATAAGAGAT+TGG 0.447043 7.3:+30387108 MS.gene42162:CDS
TATGGAAATTGAGGATTTGA+AGG 0.449937 7.3:+30387507 MS.gene42162:CDS
TATGAAGAAACAAATATTGA+AGG 0.453822 7.3:+30387093 MS.gene42162:CDS
GGACCAATTCAGAGCTGTAC+TGG 0.459816 7.3:+30386994 MS.gene42162:CDS
AACAAAAGATATGGAAATTG+AGG 0.463508 7.3:+30387435 MS.gene42162:CDS
TCGCTCTGCTTTACTTGGGA+AGG 0.491109 7.3:+30386964 MS.gene42162:CDS
GCTATGAAGTCTGTGGTTGA+TGG 0.503376 7.3:+30387622 MS.gene42162:CDS
AGCGAAGGATATGGAAATTG+AGG 0.506894 7.3:+30387498 MS.gene42162:CDS
GCCTTCGCTCTGCTTTACTT+GGG 0.510431 7.3:+30386960 MS.gene42162:CDS
TGCGAAGGATATGGAAATTG+AGG 0.517984 7.3:+30387246 MS.gene42162:CDS
TGCGAAGGATATGGAAATTG+AGG 0.517984 7.3:+30387309 MS.gene42162:CDS
TGCGAAGGATATGGAAATTG+AGG 0.517984 7.3:+30387372 MS.gene42162:CDS
CAAGGGGCAGTCTGCTGCGA+AGG 0.521712 7.3:+30387294 MS.gene42162:CDS
GCTGCCTTTCGCTATCAAGA+TGG 0.529136 7.3:+30387535 MS.gene42162:CDS
GAAGGTAAATAAGAGATTGG+AGG 0.529817 7.3:+30387111 MS.gene42162:CDS
AAAAGTATATATACGCTCAA+GGG 0.531845 7.3:+30387340 MS.gene42162:CDS
GAAAGATGTGCAGGAGGTGA+AGG 0.535532 7.3:+30386895 MS.gene42162:intron
GAAAAGTATATATACGCTCA+AGG 0.538571 7.3:+30387339 MS.gene42162:CDS
TGGTGCGAAATTATCGAAAG+AGG 0.548189 7.3:+30386728 MS.gene42162:CDS
CAAGGGGCAATCTGCTGCGA+AGG 0.550064 7.3:+30387357 MS.gene42162:CDS
ACAGACTGCTCTGACTCAGT+TGG 0.550586 7.3:+30387138 MS.gene42162:CDS
ATTGCTCCAACATCTGCTAC+AGG 0.552341 7.3:+30386687 MS.gene42162:CDS
TGCAGGAGGTGAAGGAGTCT+GGG 0.554625 7.3:+30386903 MS.gene42162:intron
AAAGTGTAGATACACTCAAA+GGG 0.554726 7.3:+30387215 MS.gene42162:CDS
GGAGGTGAAGGAGTCTGGGT+TGG 0.555934 7.3:+30386907 MS.gene42162:intron
AAAAGTGTAGATACACTCAA+AGG 0.556290 7.3:+30387214 MS.gene42162:CDS
CAAGGGGCAATCCGCAGCGA+AGG 0.556340 7.3:+30387483 MS.gene42162:CDS
CAAAGGGAAATCTGCTGCGA+AGG 0.558516 7.3:+30387231 MS.gene42162:CDS
GATTGTCCTGTAGCAGATGT+TGG 0.562417 7.3:-30386693 None:intergenic
TGTGGTGTTCAGTCTGAAGC+TGG 0.566521 7.3:+30386651 MS.gene42162:intron
GAAAAGTATAGATACGCTCA+AGG 0.571373 7.3:+30387276 MS.gene42162:CDS
AAAACCATCTTGATAGCGAA+AGG 0.577594 7.3:-30387539 None:intergenic
GACCAATTCAGAGCTGTACT+GGG 0.593790 7.3:+30386995 MS.gene42162:CDS
TGGTTGAATTCAGTTCATCT+CGG 0.603175 7.3:-30387033 None:intergenic
AAAAGTATAGATACGCTCAA+GGG 0.604119 7.3:+30387277 MS.gene42162:CDS
AAAGCGTAGATATGCTCAAG+AGG 0.605173 7.3:+30387404 MS.gene42162:CDS
GCAAAGTATAGATACGCTCA+AGG 0.611265 7.3:+30387465 MS.gene42162:CDS
CAAAGTATAGATACGCTCAA+GGG 0.614983 7.3:+30387466 MS.gene42162:CDS
AAAGTATATATACGCTCAAG+GGG 0.615609 7.3:+30387341 MS.gene42162:CDS
TCCATCGTTTGATTTGTCGG+AGG 0.626060 7.3:+30387594 MS.gene42162:CDS
AAAGTATAGATACGCTCAAG+GGG 0.633488 7.3:+30387278 MS.gene42162:CDS
TCCCAAGTAAAGCAGAGCGA+AGG 0.640241 7.3:-30386961 None:intergenic
AAAGTATAGATACGCTCAAG+GGG 0.641841 7.3:+30387467 MS.gene42162:CDS
AAAAGCTAGTGAGGTGCTTG+TGG 0.653025 7.3:+30386931 MS.gene42162:CDS
GGCTGACGCTATGAAGTCTG+TGG 0.664603 7.3:+30387615 MS.gene42162:CDS
GCCTCCGACAAATCAAACGA+TGG 0.667550 7.3:-30387595 None:intergenic
AATTTCCATATCCTTCGCTG+CGG 0.705001 7.3:-30387494 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATATAAACATTGCTATTT+TGG + chr7.3:30386776-30386795 MS.gene42162:intron 15.0%
!! TATGAAGAAACAAATATTGA+AGG + chr7.3:30387093-30387112 MS.gene42162:CDS 20.0%
! AACAAAAGATATGGAAATTG+AGG + chr7.3:30387435-30387454 MS.gene42162:CDS 25.0%
! ATTGAAGGTAAATAAGAGAT+TGG + chr7.3:30387108-30387127 MS.gene42162:CDS 25.0%
!! AAAAGTATATATACGCTCAA+GGG + chr7.3:30387340-30387359 MS.gene42162:CDS 25.0%
!! AATTTCCATATCTTTTGTTG+CGG - chr7.3:30387434-30387453 None:intergenic 25.0%
!!! GCATATAAACATTGCTATTT+TGG + chr7.3:30386805-30386824 MS.gene42162:intron 25.0%
!!! GGCTATTTTCAATTTTTCTT+TGG - chr7.3:30387056-30387075 None:intergenic 25.0%
!!! TTTGTTGCTTTGACAAATAT+TGG + chr7.3:30386627-30386646 MS.gene42162:intron 25.0%
AAAATAGCCAATGATACTCT+TGG + chr7.3:30387067-30387086 MS.gene42162:CDS 30.0%
AAAGTATATATACGCTCAAG+GGG + chr7.3:30387341-30387360 MS.gene42162:CDS 30.0%
AAAGTGTAGATACACTCAAA+GGG + chr7.3:30387215-30387234 MS.gene42162:CDS 30.0%
ACAAATCAAACGATGGAAAT+AGG - chr7.3:30387591-30387610 None:intergenic 30.0%
GAAAAAGACTGAAAATAACG+AGG + chr7.3:30387162-30387181 MS.gene42162:CDS 30.0%
TATGGAAATTGAGGATTTGA+AGG + chr7.3:30387255-30387274 MS.gene42162:CDS 30.0%
! AAAAGTATAGATACGCTCAA+GGG + chr7.3:30387277-30387296 MS.gene42162:CDS 30.0%
! AAAAGTGTAGATACACTCAA+AGG + chr7.3:30387214-30387233 MS.gene42162:CDS 30.0%
! CAGACGTTATCTTTTTATTC+TGG + chr7.3:30386853-30386872 MS.gene42162:intron 30.0%
! GAAAAGTATATATACGCTCA+AGG + chr7.3:30387339-30387358 MS.gene42162:CDS 30.0%
! TGAAAATAACGAGGTTTTTG+AGG + chr7.3:30387171-30387190 MS.gene42162:CDS 30.0%
!! ATTTCCATCGTTTGATTTGT+CGG + chr7.3:30387591-30387610 MS.gene42162:CDS 30.0%
!!! AACATTGCTATTTTGGTGTT+TGG + chr7.3:30386783-30386802 MS.gene42162:intron 30.0%
!!! CTATCAAGATGGTTTTGATA+AGG + chr7.3:30387546-30387565 MS.gene42162:CDS 30.0%
AAAGTATAGATACGCTCAAG+GGG + chr7.3:30387278-30387297 MS.gene42162:CDS 35.0%
CAAAGTATAGATACGCTCAA+GGG + chr7.3:30387466-30387485 MS.gene42162:CDS 35.0%
GAAGGTAAATAAGAGATTGG+AGG + chr7.3:30387111-30387130 MS.gene42162:CDS 35.0%
GCTTTGACAAATATTGGATG+TGG + chr7.3:30386633-30386652 MS.gene42162:intron 35.0%
GGTTAATAGCGATTATTTGC+TGG + chr7.3:30386749-30386768 MS.gene42162:intron 35.0%
TGGTTGAATTCAGTTCATCT+CGG - chr7.3:30387036-30387055 None:intergenic 35.0%
TTTACTTGGGAAGGTGATTA+CGG + chr7.3:30386973-30386992 MS.gene42162:CDS 35.0%
! GAAAAGTATAGATACGCTCA+AGG + chr7.3:30387276-30387295 MS.gene42162:CDS 35.0%
! TAGGATGTGAACTTGTTCTA+TGG - chr7.3:30387572-30387591 None:intergenic 35.0%
!! AAAACCATCTTGATAGCGAA+AGG - chr7.3:30387542-30387561 None:intergenic 35.0%
!! AAATAACGAGGTTTTTGAGG+AGG + chr7.3:30387174-30387193 MS.gene42162:CDS 35.0%
!! CATATCTCCAAGAGTATCAT+TGG - chr7.3:30387077-30387096 None:intergenic 35.0%
!! GATAATTTCGCACCACTTTT+TGG - chr7.3:30386723-30386742 None:intergenic 35.0%
AAAGCGTAGATATGCTCAAG+AGG + chr7.3:30387404-30387423 MS.gene42162:CDS 40.0%
AATTTCCATATCCTTCGCTG+CGG - chr7.3:30387497-30387516 None:intergenic 40.0%
AGCGAAGGATATGGAAATTG+AGG + chr7.3:30387498-30387517 MS.gene42162:CDS 40.0%
GCAAAGTATAGATACGCTCA+AGG + chr7.3:30387465-30387484 MS.gene42162:CDS 40.0%
GCAATCCGCAACAAAAGATA+TGG + chr7.3:30387426-30387445 MS.gene42162:CDS 40.0%
GGACAATCTCAACCAAAAAG+TGG + chr7.3:30386708-30386727 MS.gene42162:CDS 40.0%
TGCGAAGGATATGGAAATTG+AGG + chr7.3:30387246-30387265 MS.gene42162:CDS 40.0%
TGGTGCGAAATTATCGAAAG+AGG + chr7.3:30386728-30386747 MS.gene42162:CDS 40.0%
AAGCTTCACGAAAGATGTGC+AGG + chr7.3:30386886-30386905 MS.gene42162:intron 45.0%
ATTGCTCCAACATCTGCTAC+AGG + chr7.3:30386687-30386706 MS.gene42162:CDS 45.0%
GAAATCTGCTGCGAAGGATA+TGG + chr7.3:30387237-30387256 MS.gene42162:CDS 45.0%
GACCAATTCAGAGCTGTACT+GGG + chr7.3:30386995-30387014 MS.gene42162:CDS 45.0%
GATTGTCCTGTAGCAGATGT+TGG - chr7.3:30386696-30386715 None:intergenic 45.0%
GCTATGAAGTCTGTGGTTGA+TGG + chr7.3:30387622-30387641 MS.gene42162:CDS 45.0%
! AAAAGCTAGTGAGGTGCTTG+TGG + chr7.3:30386931-30386950 MS.gene42162:CDS 45.0%
! TGGGTTGGAAAAAGCTAGTG+AGG + chr7.3:30386922-30386941 MS.gene42162:CDS 45.0%
!! GTTTTTGAGGAGGAGGTTGT+TGG + chr7.3:30387184-30387203 MS.gene42162:CDS 45.0%
!! TAACGAGGTTTTTGAGGAGG+AGG + chr7.3:30387177-30387196 MS.gene42162:CDS 45.0%
!! TCCATCGTTTGATTTGTCGG+AGG + chr7.3:30387594-30387613 MS.gene42162:CDS 45.0%
ACAGACTGCTCTGACTCAGT+TGG + chr7.3:30387138-30387157 MS.gene42162:CDS 50.0%
AGCCTTCGCTCTGCTTTACT+TGG + chr7.3:30386959-30386978 MS.gene42162:CDS 50.0%
CAAAGGGAAATCTGCTGCGA+AGG + chr7.3:30387231-30387250 MS.gene42162:CDS 50.0%
CTTCACGAAAGATGTGCAGG+AGG + chr7.3:30386889-30386908 MS.gene42162:intron 50.0%
GAAAGATGTGCAGGAGGTGA+AGG + chr7.3:30386895-30386914 MS.gene42162:intron 50.0%
GCAATCTGCTGCGAAGGATA+TGG + chr7.3:30387363-30387382 MS.gene42162:CDS 50.0%
GCCTCCGACAAATCAAACGA+TGG - chr7.3:30387598-30387617 None:intergenic 50.0%
GCCTTCGCTCTGCTTTACTT+GGG + chr7.3:30386960-30386979 MS.gene42162:CDS 50.0%
GCTGCCTTTCGCTATCAAGA+TGG + chr7.3:30387535-30387554 MS.gene42162:CDS 50.0%
GGACCAATTCAGAGCTGTAC+TGG + chr7.3:30386994-30387013 MS.gene42162:CDS 50.0%
TCCCAAGTAAAGCAGAGCGA+AGG - chr7.3:30386964-30386983 None:intergenic 50.0%
TCGCTCTGCTTTACTTGGGA+AGG + chr7.3:30386964-30386983 MS.gene42162:CDS 50.0%
! TGTGGTGTTCAGTCTGAAGC+TGG + chr7.3:30386651-30386670 MS.gene42162:intron 50.0%
!! GTCCCAGTACAGCTCTGAAT+TGG - chr7.3:30387000-30387019 None:intergenic 50.0%
GCAATCCGCAGCGAAGGATA+TGG + chr7.3:30387489-30387508 MS.gene42162:CDS 55.0%
GCAGTCTGCTGCGAAGGATA+TGG + chr7.3:30387300-30387319 MS.gene42162:CDS 55.0%
GGCTGACGCTATGAAGTCTG+TGG + chr7.3:30387615-30387634 MS.gene42162:CDS 55.0%
TGCAGGAGGTGAAGGAGTCT+GGG + chr7.3:30386903-30386922 MS.gene42162:intron 55.0%
CAAGGGGCAATCTGCTGCGA+AGG + chr7.3:30387357-30387376 MS.gene42162:CDS 60.0%
GGAGGTGAAGGAGTCTGGGT+TGG + chr7.3:30386907-30386926 MS.gene42162:intron 60.0%
GTGCAGGAGGTGAAGGAGTC+TGG + chr7.3:30386902-30386921 MS.gene42162:intron 60.0%
CAAGGGGCAATCCGCAGCGA+AGG + chr7.3:30387483-30387502 MS.gene42162:CDS 65.0%
CAAGGGGCAGTCTGCTGCGA+AGG + chr7.3:30387294-30387313 MS.gene42162:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 30386599 30387657 30386599 ID=MS.gene42162
chr7.3 mRNA 30386599 30387657 30386599 ID=MS.gene42162.t1;Parent=MS.gene42162
chr7.3 exon 30386599 30386601 30386599 ID=MS.gene42162.t1.exon1;Parent=MS.gene42162.t1
chr7.3 CDS 30386599 30386601 30386599 ID=cds.MS.gene42162.t1;Parent=MS.gene42162.t1
chr7.3 exon 30386663 30386749 30386663 ID=MS.gene42162.t1.exon2;Parent=MS.gene42162.t1
chr7.3 CDS 30386663 30386749 30386663 ID=cds.MS.gene42162.t1;Parent=MS.gene42162.t1
chr7.3 exon 30386908 30387657 30386908 ID=MS.gene42162.t1.exon3;Parent=MS.gene42162.t1
chr7.3 CDS 30386908 30387657 30386908 ID=cds.MS.gene42162.t1;Parent=MS.gene42162.t1
Gene Sequence

>MS.gene42162

ATGGTTCTGATGCTTATTGTTTTTGATATTTGTTGCTTTGACAAATATTGGATGTGGTGTTCAGTCTGAAGCTGGTGAAAAACAAGTGATTGCTCCAACATCTGCTACAGGACAATCTCAACCAAAAAGTGGTGCGAAATTATCGAAAGAGGTTAATAGCGATTATTTGCTGGCTGAAAATATAAACATTGCTATTTTGGTGTTTAGCATATAAACATTGCTATTTTGGTGTTTGGCACACAAATAAAATAAAGCAGACGTTATCTTTTTATTCTGGACAGCAATCTAAGCTTCACGAAAGATGTGCAGGAGGTGAAGGAGTCTGGGTTGGAAAAAGCTAGTGAGGTGCTTGTGGCGTACAGCCTTCGCTCTGCTTTACTTGGGAAGGTGATTACGGACCAATTCAGAGCTGTACTGGGACATGAAAGAAACTGCCGAGATGAACTGAATTCAACCAAAGAAAAATTGAAAATAGCCAATGATACTCTTGGAGATATGAAGAAACAAATATTGAAGGTAAATAAGAGATTGGAGGAAGAACAGACTGCTCTGACTCAGTTGGAGAAAAAGACTGAAAATAACGAGGTTTTTGAGGAGGAGGTTGTTGGCTTGAAGAAAAGTGTAGATACACTCAAAGGGAAATCTGCTGCGAAGGATATGGAAATTGAGGATTTGAAGAAAAGTATAGATACGCTCAAGGGGCAGTCTGCTGCGAAGGATATGGAAATTGAGGATTTGAAGAAAAGTATATATACGCTCAAGGGGCAATCTGCTGCGAAGGATATGGAAATTGAGGATTTGAAGAAAAGCGTAGATATGCTCAAGAGGCAATCCGCAACAAAAGATATGGAAATTGAGGATTTGAAGCAAAGTATAGATACGCTCAAGGGGCAATCCGCAGCGAAGGATATGGAAATTGAGGATTTGAAGGTAGACGCTGCCTTTCGCTATCAAGATGGTTTTGATAAGGCCATAGAACAAGTTCACATCCTATTTCCATCGTTTGATTTGTCGGAGGCTGACGCTATGAAGTCTGTGGTTGATGGAAAACTAGTATAA

Protein sequence

>MS.gene42162.t1

MSEAGEKQVIAPTSATGQSQPKSGAKLSKEEVKESGLEKASEVLVAYSLRSALLGKVITDQFRAVLGHERNCRDELNSTKEKLKIANDTLGDMKKQILKVNKRLEEEQTALTQLEKKTENNEVFEEEVVGLKKSVDTLKGKSAAKDMEIEDLKKSIDTLKGQSAAKDMEIEDLKKSIYTLKGQSAAKDMEIEDLKKSVDMLKRQSATKDMEIEDLKQSIDTLKGQSAAKDMEIEDLKVDAAFRYQDGFDKAIEQVHILFPSFDLSEADAMKSVVDGKLV