AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene46355


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46355.t1 MTR_4g098710 93.827 81 5 0 1 81 1 81 2.42e-40 127
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 14 sgRNAs with CRISPR-Local

Find 13 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAATTCAGAATCTTATC+TGG 0.320719 8.4:-24315577 None:intergenic
GTGATCAGGATGAACAGTTT+AGG 0.424887 8.4:+24315647 MS.gene46355:CDS
TGTTCATCCTGATCACAGTT+TGG 0.447960 8.4:-24315640 None:intergenic
TGTGTTGCCAAACTGTGATC+AGG 0.505518 8.4:+24315633 MS.gene46355:CDS
TCACTAGACTCCTTCTTGCT+CGG 0.520514 8.4:-24315511 None:intergenic
AAGATCGGATCGATTGCAAT+GGG 0.546672 8.4:+24315712 MS.gene46355:CDS
TCTCAGAGCAAGAAATCAGA+TGG 0.548303 8.4:+24315607 MS.gene46355:CDS
ATCGATTGCAATGGGAGTCT+AGG 0.564890 8.4:+24315720 MS.gene46355:CDS
CAAGATCGGATCGATTGCAA+TGG 0.580943 8.4:+24315711 MS.gene46355:CDS
GAATCAACTGATGTCAAGAT+CGG 0.588286 8.4:+24315697 MS.gene46355:CDS
CAAGAAGGAGTCTAGTGAAG+TGG 0.591426 8.4:+24315516 MS.gene46355:CDS
TAGCAAGTCACCGAGCAAGA+AGG 0.600583 8.4:+24315501 MS.gene46355:CDS
CTCAGAGCAAGAAATCAGAT+GGG 0.605996 8.4:+24315608 MS.gene46355:CDS
TCAGAGCAAGAAATCAGATG+GGG 0.702066 8.4:+24315609 MS.gene46355:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTTGAATTCAGAATCTTATC+TGG - chr8.4:24315580-24315599 None:intergenic 25.0%
GAATCAACTGATGTCAAGAT+CGG + chr8.4:24315697-24315716 MS.gene46355:CDS 35.0%
AAGATCGGATCGATTGCAAT+GGG + chr8.4:24315712-24315731 MS.gene46355:CDS 40.0%
CTCAGAGCAAGAAATCAGAT+GGG + chr8.4:24315608-24315627 MS.gene46355:CDS 40.0%
GTGATCAGGATGAACAGTTT+AGG + chr8.4:24315647-24315666 MS.gene46355:CDS 40.0%
TCAGAGCAAGAAATCAGATG+GGG + chr8.4:24315609-24315628 MS.gene46355:CDS 40.0%
TCTCAGAGCAAGAAATCAGA+TGG + chr8.4:24315607-24315626 MS.gene46355:CDS 40.0%
TGTTCATCCTGATCACAGTT+TGG - chr8.4:24315643-24315662 None:intergenic 40.0%
CAAGAAGGAGTCTAGTGAAG+TGG + chr8.4:24315516-24315535 MS.gene46355:CDS 45.0%
CAAGATCGGATCGATTGCAA+TGG + chr8.4:24315711-24315730 MS.gene46355:CDS 45.0%
TCACTAGACTCCTTCTTGCT+CGG - chr8.4:24315514-24315533 None:intergenic 45.0%
TGTGTTGCCAAACTGTGATC+AGG + chr8.4:24315633-24315652 MS.gene46355:CDS 45.0%
TAGCAAGTCACCGAGCAAGA+AGG + chr8.4:24315501-24315520 MS.gene46355:CDS 50.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 24315496 24315741 24315496 ID=MS.gene46355
chr8.4 mRNA 24315496 24315741 24315496 ID=MS.gene46355.t1;Parent=MS.gene46355
chr8.4 exon 24315496 24315741 24315496 ID=MS.gene46355.t1.exon1;Parent=MS.gene46355.t1
chr8.4 CDS 24315496 24315741 24315496 ID=cds.MS.gene46355.t1;Parent=MS.gene46355.t1
Gene Sequence

>MS.gene46355

ATGGATAGCAAGTCACCGAGCAAGAAGGAGTCTAGTGAAGTGGCAGCACGCGAGTCGCTTATTGCTATTTCTAACTCTTCACCAGATAAGATTCTGAATTCAAATTCTGTTTCTCAGAGCAAGAAATCAGATGGGGCTGTGTTGCCAAACTGTGATCAGGATGAACAGTTTAGGTCTGAACTGATTTCAATTTCGTATGCTGAATCAACTGATGTCAAGATCGGATCGATTGCAATGGGAGTCTAG

Protein sequence

>MS.gene46355.t1

MDSKSPSKKESSEVAARESLIAISNSSPDKILNSNSVSQSKKSDGAVLPNCDQDEQFRSELISISYAESTDVKIGSIAMGV