Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48438.t1 | XP_004501619.1 | 82.6 | 259 | 28 | 3 | 2 | 249 | 19 | 271 | 8.90E-63 | 250.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48438.t1 | Q9SB61 | 48.6 | 245 | 87 | 2 | 5 | 249 | 15 | 220 | 6.1e-44 | 179.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48438.t1 | A0A1S2YAX3 | 82.6 | 259 | 28 | 3 | 2 | 249 | 19 | 271 | 6.4e-63 | 250.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene48438.t1 | TF | zf-HD |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049260 | MS.gene48438 | 0.817944 | 2.54E-52 | -1.69E-46 |
| MS.gene049351 | MS.gene48438 | 0.803844 | 2.87E-49 | -1.69E-46 |
| MS.gene049491 | MS.gene48438 | 0.811328 | 7.40E-51 | -1.69E-46 |
| MS.gene049661 | MS.gene48438 | 0.807096 | 5.97E-50 | -1.69E-46 |
| MS.gene049662 | MS.gene48438 | 0.808847 | 2.53E-50 | -1.69E-46 |
| MS.gene049758 | MS.gene48438 | 0.859387 | 4.29E-63 | -1.69E-46 |
| MS.gene050076 | MS.gene48438 | 0.806208 | 9.20E-50 | -1.69E-46 |
| MS.gene050099 | MS.gene48438 | 0.817101 | 3.93E-52 | -1.69E-46 |
| MS.gene050128 | MS.gene48438 | 0.895705 | 7.44E-76 | -1.69E-46 |
| MS.gene050301 | MS.gene48438 | 0.854211 | 1.43E-61 | -1.69E-46 |
| MS.gene050397 | MS.gene48438 | 0.823559 | 1.30E-53 | -1.69E-46 |
| MS.gene050404 | MS.gene48438 | 0.836681 | 8.11E-57 | -1.69E-46 |
| MS.gene050406 | MS.gene48438 | 0.829407 | 5.24E-55 | -1.69E-46 |
| MS.gene050464 | MS.gene48438 | 0.811059 | 8.46E-51 | -1.69E-46 |
| MS.gene050529 | MS.gene48438 | 0.83587 | 1.30E-56 | -1.69E-46 |
| MS.gene050767 | MS.gene48438 | 0.814289 | 1.66E-51 | -1.69E-46 |
| MS.gene050770 | MS.gene48438 | 0.81467 | 1.37E-51 | -1.69E-46 |
| MS.gene05101 | MS.gene48438 | 0.828735 | 7.62E-55 | -1.69E-46 |
| MS.gene051471 | MS.gene48438 | 0.859167 | 4.99E-63 | -1.69E-46 |
| MS.gene051662 | MS.gene48438 | 0.801808 | 7.56E-49 | -1.69E-46 |
| MS.gene051663 | MS.gene48438 | 0.801994 | 6.92E-49 | -1.69E-46 |
| MS.gene051860 | MS.gene48438 | 0.800454 | 1.43E-48 | -1.69E-46 |
| MS.gene052078 | MS.gene48438 | 0.883791 | 3.33E-71 | -1.69E-46 |
| MS.gene052178 | MS.gene48438 | 0.937122 | 5.78E-98 | -1.69E-46 |
| MS.gene052188 | MS.gene48438 | 0.8218 | 3.33E-53 | -1.69E-46 |
| MS.gene052290 | MS.gene48438 | 0.831345 | 1.76E-55 | -1.69E-46 |
| MS.gene052310 | MS.gene48438 | 0.847986 | 8.17E-60 | -1.69E-46 |
| MS.gene052326 | MS.gene48438 | 0.827675 | 1.37E-54 | -1.69E-46 |
| MS.gene052340 | MS.gene48438 | 0.846782 | 1.75E-59 | -1.69E-46 |
| MS.gene053073 | MS.gene48438 | 0.821767 | 3.39E-53 | -1.69E-46 |
| MS.gene05316 | MS.gene48438 | 0.806993 | 6.28E-50 | -1.69E-46 |
| MS.gene053209 | MS.gene48438 | 0.814847 | 1.25E-51 | -1.69E-46 |
| MS.gene053557 | MS.gene48438 | 0.879712 | 9.96E-70 | -1.69E-46 |
| MS.gene053836 | MS.gene48438 | 0.832688 | 8.20E-56 | -1.69E-46 |
| MS.gene053928 | MS.gene48438 | 0.804337 | 2.27E-49 | -1.69E-46 |
| MS.gene054011 | MS.gene48438 | 0.813255 | 2.81E-51 | -1.69E-46 |
| MS.gene054012 | MS.gene48438 | 0.807506 | 4.89E-50 | -1.69E-46 |
| MS.gene054013 | MS.gene48438 | 0.807008 | 6.23E-50 | -1.69E-46 |
| MS.gene054123 | MS.gene48438 | 0.831069 | 2.06E-55 | -1.69E-46 |
| MS.gene054214 | MS.gene48438 | 0.851979 | 6.23E-61 | -1.69E-46 |
| MS.gene054376 | MS.gene48438 | 0.823388 | 1.42E-53 | -1.69E-46 |
| MS.gene055133 | MS.gene48438 | 0.831307 | 1.80E-55 | -1.69E-46 |
| MS.gene055254 | MS.gene48438 | 0.835879 | 1.30E-56 | -1.69E-46 |
| MS.gene055277 | MS.gene48438 | 0.877601 | 5.51E-69 | -1.69E-46 |
| MS.gene055390 | MS.gene48438 | 0.833971 | 3.93E-56 | -1.69E-46 |
| MS.gene055422 | MS.gene48438 | 0.866172 | 3.46E-65 | -1.69E-46 |
| MS.gene055428 | MS.gene48438 | 0.83092 | 2.24E-55 | -1.69E-46 |
| MS.gene055497 | MS.gene48438 | -0.818519 | 1.88E-52 | -1.69E-46 |
| MS.gene055604 | MS.gene48438 | 0.833156 | 6.27E-56 | -1.69E-46 |
| MS.gene055896 | MS.gene48438 | 0.801881 | 7.30E-49 | -1.69E-46 |
| MS.gene056191 | MS.gene48438 | 0.821374 | 4.18E-53 | -1.69E-46 |
| MS.gene056314 | MS.gene48438 | 0.812821 | 3.50E-51 | -1.69E-46 |
| MS.gene056410 | MS.gene48438 | 0.825598 | 4.30E-54 | -1.69E-46 |
| MS.gene056609 | MS.gene48438 | 0.843224 | 1.60E-58 | -1.69E-46 |
| MS.gene056612 | MS.gene48438 | 0.860493 | 1.99E-63 | -1.69E-46 |
| MS.gene056772 | MS.gene48438 | 0.805006 | 1.64E-49 | -1.69E-46 |
| MS.gene05702 | MS.gene48438 | 0.837592 | 4.74E-57 | -1.69E-46 |
| MS.gene057295 | MS.gene48438 | 0.825758 | 3.94E-54 | -1.69E-46 |
| MS.gene057439 | MS.gene48438 | 0.821493 | 3.92E-53 | -1.69E-46 |
| MS.gene057449 | MS.gene48438 | 0.801322 | 9.51E-49 | -1.69E-46 |
| MS.gene057450 | MS.gene48438 | 0.813307 | 2.73E-51 | -1.69E-46 |
| MS.gene057794 | MS.gene48438 | 0.823583 | 1.28E-53 | -1.69E-46 |
| MS.gene057796 | MS.gene48438 | 0.826125 | 3.22E-54 | -1.69E-46 |
| MS.gene058002 | MS.gene48438 | 0.826909 | 2.09E-54 | -1.69E-46 |
| MS.gene058082 | MS.gene48438 | 0.813799 | 2.13E-51 | -1.69E-46 |
| MS.gene05811 | MS.gene48438 | 0.849159 | 3.87E-60 | -1.69E-46 |
| MS.gene058276 | MS.gene48438 | 0.807952 | 3.93E-50 | -1.69E-46 |
| MS.gene058293 | MS.gene48438 | 0.813224 | 2.85E-51 | -1.69E-46 |
| MS.gene058660 | MS.gene48438 | 0.835869 | 1.31E-56 | -1.69E-46 |
| MS.gene058845 | MS.gene48438 | 0.811298 | 7.51E-51 | -1.69E-46 |
| MS.gene058928 | MS.gene48438 | 0.835747 | 1.40E-56 | -1.69E-46 |
| MS.gene05907 | MS.gene48438 | 0.826307 | 2.92E-54 | -1.69E-46 |
| MS.gene059228 | MS.gene48438 | 0.852975 | 3.24E-61 | -1.69E-46 |
| MS.gene059294 | MS.gene48438 | 0.858441 | 8.22E-63 | -1.69E-46 |
| MS.gene059326 | MS.gene48438 | 0.819266 | 1.27E-52 | -1.69E-46 |
| MS.gene059518 | MS.gene48438 | 0.856579 | 2.92E-62 | -1.69E-46 |
| MS.gene059597 | MS.gene48438 | 0.825489 | 4.56E-54 | -1.69E-46 |
| MS.gene05959 | MS.gene48438 | 0.805672 | 1.19E-49 | -1.69E-46 |
| MS.gene059676 | MS.gene48438 | 0.808067 | 3.72E-50 | -1.69E-46 |
| MS.gene059692 | MS.gene48438 | 0.825631 | 4.22E-54 | -1.69E-46 |
| MS.gene059695 | MS.gene48438 | 0.816715 | 4.79E-52 | -1.69E-46 |
| MS.gene059708 | MS.gene48438 | 0.839991 | 1.14E-57 | -1.69E-46 |
| MS.gene05984 | MS.gene48438 | 0.843095 | 1.73E-58 | -1.69E-46 |
| MS.gene059894 | MS.gene48438 | 0.893293 | 7.19E-75 | -1.69E-46 |
| MS.gene059974 | MS.gene48438 | 0.862129 | 6.31E-64 | -1.69E-46 |
| MS.gene060005 | MS.gene48438 | 0.867336 | 1.48E-65 | -1.69E-46 |
| MS.gene06037 | MS.gene48438 | 0.868095 | 8.42E-66 | -1.69E-46 |
| MS.gene060476 | MS.gene48438 | 0.829586 | 4.74E-55 | -1.69E-46 |
| MS.gene060495 | MS.gene48438 | 0.802986 | 4.32E-49 | -1.69E-46 |
| MS.gene060800 | MS.gene48438 | 0.817858 | 2.65E-52 | -1.69E-46 |
| MS.gene061049 | MS.gene48438 | 0.873972 | 9.71E-68 | -1.69E-46 |
| MS.gene061051 | MS.gene48438 | -0.835325 | 1.79E-56 | -1.69E-46 |
| MS.gene061112 | MS.gene48438 | 0.851714 | 7.41E-61 | -1.69E-46 |
| MS.gene061265 | MS.gene48438 | 0.843866 | 1.08E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48438.t1 | MTR_3g100650 | 95.219 | 251 | 5 | 2 | 1 | 249 | 29 | 274 | 4.27e-177 | 488 |
| MS.gene48438.t1 | MTR_5g065440 | 65.625 | 256 | 69 | 5 | 6 | 249 | 20 | 268 | 1.84e-99 | 291 |
| MS.gene48438.t1 | MTR_5g032600 | 54.167 | 216 | 70 | 7 | 46 | 249 | 74 | 272 | 5.94e-62 | 196 |
| MS.gene48438.t1 | MTR_4g134580 | 43.515 | 239 | 92 | 6 | 49 | 248 | 112 | 346 | 1.53e-55 | 182 |
| MS.gene48438.t1 | MTR_1g077020 | 44.554 | 202 | 91 | 3 | 47 | 248 | 6 | 186 | 4.75e-53 | 170 |
| MS.gene48438.t1 | MTR_5g020510 | 43.333 | 210 | 105 | 3 | 45 | 248 | 94 | 295 | 5.09e-48 | 160 |
| MS.gene48438.t1 | MTR_2g076780 | 43.541 | 209 | 99 | 5 | 50 | 248 | 37 | 236 | 7.76e-48 | 158 |
| MS.gene48438.t1 | MTR_8g069080 | 42.788 | 208 | 103 | 3 | 49 | 248 | 68 | 267 | 5.93e-47 | 157 |
| MS.gene48438.t1 | MTR_7g010300 | 41.121 | 214 | 98 | 5 | 47 | 248 | 85 | 282 | 4.22e-46 | 157 |
| MS.gene48438.t1 | MTR_6g012280 | 41.463 | 205 | 103 | 5 | 50 | 248 | 91 | 284 | 6.32e-39 | 138 |
| MS.gene48438.t1 | MTR_8g069380 | 51.163 | 86 | 41 | 1 | 164 | 249 | 31 | 115 | 1.58e-21 | 86.7 |
| MS.gene48438.t1 | MTR_3g039810 | 61.290 | 62 | 24 | 0 | 48 | 109 | 18 | 79 | 6.70e-21 | 84.3 |
| MS.gene48438.t1 | MTR_8g051480 | 54.667 | 75 | 33 | 1 | 174 | 248 | 55 | 128 | 7.43e-21 | 85.5 |
| MS.gene48438.t1 | MTR_8g442330 | 34.756 | 164 | 68 | 5 | 60 | 223 | 15 | 139 | 2.63e-20 | 84.3 |
| MS.gene48438.t1 | MTR_7g070643 | 69.492 | 59 | 15 | 1 | 30 | 88 | 65 | 120 | 1.28e-19 | 84.0 |
| MS.gene48438.t1 | MTR_8g467340 | 53.968 | 63 | 29 | 0 | 186 | 248 | 271 | 333 | 1.78e-18 | 84.0 |
| MS.gene48438.t1 | MTR_8g467340 | 56.364 | 55 | 22 | 1 | 47 | 99 | 61 | 115 | 4.74e-13 | 68.2 |
| MS.gene48438.t1 | MTR_6g080300 | 80.952 | 42 | 8 | 0 | 47 | 88 | 51 | 92 | 6.52e-18 | 76.6 |
| MS.gene48438.t1 | MTR_6g073013 | 55.000 | 60 | 26 | 1 | 186 | 244 | 101 | 160 | 6.18e-17 | 76.6 |
| MS.gene48438.t1 | MTR_6g073013 | 52.000 | 50 | 23 | 1 | 50 | 98 | 19 | 68 | 8.33e-11 | 59.7 |
| MS.gene48438.t1 | MTR_6g012220 | 55.769 | 52 | 19 | 1 | 47 | 98 | 18 | 65 | 4.08e-13 | 63.5 |
| MS.gene48438.t1 | MTR_2g007810 | 93.103 | 29 | 2 | 0 | 60 | 88 | 19 | 47 | 1.96e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48438.t1 | AT4G24660 | 53.202 | 203 | 65 | 5 | 48 | 249 | 47 | 220 | 2.76e-66 | 205 |
| MS.gene48438.t1 | AT4G24660 | 52.261 | 199 | 65 | 5 | 48 | 245 | 47 | 216 | 4.67e-63 | 197 |
| MS.gene48438.t1 | AT2G02540 | 38.314 | 261 | 130 | 6 | 7 | 248 | 36 | 284 | 5.39e-60 | 192 |
| MS.gene48438.t1 | AT2G02540 | 38.314 | 261 | 130 | 6 | 7 | 248 | 36 | 284 | 5.39e-60 | 192 |
| MS.gene48438.t1 | AT1G14440 | 45.366 | 205 | 96 | 4 | 48 | 248 | 88 | 280 | 1.96e-54 | 177 |
| MS.gene48438.t1 | AT1G14440 | 45.366 | 205 | 96 | 4 | 48 | 248 | 88 | 280 | 1.96e-54 | 177 |
| MS.gene48438.t1 | AT5G65410 | 45.536 | 224 | 74 | 5 | 40 | 249 | 65 | 254 | 6.51e-51 | 167 |
| MS.gene48438.t1 | AT2G18350 | 47.291 | 203 | 87 | 4 | 46 | 248 | 78 | 260 | 2.51e-50 | 166 |
| MS.gene48438.t1 | AT3G50890 | 43.216 | 199 | 98 | 3 | 49 | 247 | 59 | 242 | 1.92e-46 | 155 |
| MS.gene48438.t1 | AT1G75240 | 41.126 | 231 | 108 | 2 | 47 | 249 | 73 | 303 | 4.63e-46 | 156 |
| MS.gene48438.t1 | AT5G15210 | 38.863 | 211 | 100 | 5 | 40 | 242 | 46 | 235 | 5.49e-38 | 134 |
| MS.gene48438.t1 | AT1G69600 | 40.291 | 206 | 96 | 7 | 48 | 245 | 29 | 215 | 7.36e-37 | 130 |
| MS.gene48438.t1 | AT3G28920 | 33.333 | 231 | 92 | 4 | 48 | 245 | 50 | 251 | 4.61e-34 | 125 |
| MS.gene48438.t1 | AT5G60480 | 37.931 | 203 | 88 | 4 | 47 | 243 | 34 | 204 | 1.32e-32 | 119 |
| MS.gene48438.t1 | AT5G42780 | 31.553 | 206 | 100 | 5 | 50 | 245 | 64 | 238 | 1.36e-24 | 98.6 |
| MS.gene48438.t1 | AT1G74660 | 69.355 | 62 | 19 | 0 | 48 | 109 | 37 | 98 | 1.36e-24 | 94.4 |
| MS.gene48438.t1 | AT1G74660 | 72.414 | 58 | 16 | 0 | 48 | 105 | 37 | 94 | 2.50e-24 | 93.6 |
| MS.gene48438.t1 | AT3G28917 | 66.667 | 60 | 19 | 1 | 47 | 105 | 30 | 89 | 7.90e-22 | 87.0 |
| MS.gene48438.t1 | AT1G18835 | 60.317 | 63 | 22 | 1 | 47 | 109 | 23 | 82 | 1.16e-19 | 81.3 |
| MS.gene48438.t1 | AT5G39760 | 53.846 | 65 | 30 | 0 | 184 | 248 | 198 | 262 | 2.67e-19 | 85.9 |
| MS.gene48438.t1 | AT1G14687 | 26.866 | 201 | 104 | 7 | 46 | 242 | 3 | 164 | 5.14e-14 | 68.6 |
Find 82 sgRNAs with CRISPR-Local
Find 91 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGCATTTGCGGAGAAAAT+TGG | 0.226300 | 3.1:-80006061 | MS.gene48438:CDS |
| TAACATGCCACCGGCGTAAC+AGG | 0.280829 | 3.1:+80006655 | None:intergenic |
| TTTCTTTGCGGTGAAAGTTC+CGG | 0.342082 | 3.1:+80006389 | None:intergenic |
| GGTGGTAGTGGAGGAGGAAG+TGG | 0.392563 | 3.1:-80006136 | MS.gene48438:CDS |
| TATCAAGAGACATGTGCTTA+AGG | 0.397983 | 3.1:-80005981 | MS.gene48438:CDS |
| AGAGACATGTGCTTAAGGTT+TGG | 0.408486 | 3.1:-80005976 | MS.gene48438:CDS |
| GGATAAGATGCTTGCATTTG+CGG | 0.410009 | 3.1:-80006071 | MS.gene48438:CDS |
| CCACCACCACCGCTGCTGCT+TGG | 0.417998 | 3.1:+80006157 | None:intergenic |
| AGCAGCAGCGGTGGTGGTGG+TGG | 0.434714 | 3.1:-80006154 | MS.gene48438:CDS |
| AAAATTCACACAGGAACAAA+AGG | 0.449497 | 3.1:-80006092 | MS.gene48438:CDS |
| AACGGACGATGTTGTGATAT+CGG | 0.449782 | 3.1:+80006241 | None:intergenic |
| TTGTATCTGACCGTTCCTGC+TGG | 0.450197 | 3.1:+80006535 | None:intergenic |
| CACAATAACAAGCACACTCT+TGG | 0.453753 | 3.1:-80005950 | MS.gene48438:CDS |
| ATTGATGGTGGTGGTGTTGA+GGG | 0.453905 | 3.1:+80006332 | None:intergenic |
| GGTGGTGGTGGTAGTGGAGG+AGG | 0.457454 | 3.1:-80006142 | MS.gene48438:CDS |
| TTGTGATATCGGTGGAGTTA+CGG | 0.462629 | 3.1:+80006252 | None:intergenic |
| ATTCACCGTCGATTTCTTTG+CGG | 0.470970 | 3.1:+80006377 | None:intergenic |
| CCAAGCAGCAGCGGTGGTGG+TGG | 0.471348 | 3.1:-80006157 | MS.gene48438:CDS |
| TTGCATTTGCGGAGAAAATT+GGG | 0.474261 | 3.1:-80006060 | MS.gene48438:CDS |
| GTTGAGGGTGATGAGACCGC+TGG | 0.475704 | 3.1:+80006347 | None:intergenic |
| TGGTGGAAACCAGGCGGAGG+AGG | 0.482300 | 3.1:+80006280 | None:intergenic |
| CGCTTGGAAACTCAGGAGTT+AGG | 0.485005 | 3.1:-80006627 | MS.gene48438:CDS |
| AATTGATGGTGGTGGTGTTG+AGG | 0.486125 | 3.1:+80006331 | None:intergenic |
| AACGCTGCACGGTGGTAGTA+AGG | 0.486813 | 3.1:+80006304 | None:intergenic |
| TCCACCAGCTGCATCGAGTA+GGG | 0.488823 | 3.1:-80006212 | MS.gene48438:CDS |
| AATGGGGTTCCCTGTTACGC+CGG | 0.489920 | 3.1:-80006665 | MS.gene48438:CDS |
| GCAGCGTTACCTCCTCCGCC+TGG | 0.493199 | 3.1:-80006289 | MS.gene48438:CDS |
| TTCCAGTGTTCCTTCTTCTC+CGG | 0.499051 | 3.1:+80006441 | None:intergenic |
| GAGGAAGTGGAACGAAGAAA+AGG | 0.501558 | 3.1:-80006123 | MS.gene48438:CDS |
| ACAGGCGGTGGTGTCAAAAG+TGG | 0.503495 | 3.1:-80006559 | MS.gene48438:CDS |
| AATCCAAGCAGCAGCGGTGG+TGG | 0.510288 | 3.1:-80006160 | MS.gene48438:CDS |
| TTGAGGGTGATGAGACCGCT+GGG | 0.515445 | 3.1:+80006348 | None:intergenic |
| GGTGGAATCGGTTCAGCGGA+CGG | 0.518412 | 3.1:-80006592 | MS.gene48438:CDS |
| GGTGTCAAAAGTGGGCCAGC+AGG | 0.527350 | 3.1:-80006550 | MS.gene48438:CDS |
| TGGTGGTGGAAACCAGGCGG+AGG | 0.539701 | 3.1:+80006277 | None:intergenic |
| GGTAGTAAGGTGATAATTGA+TGG | 0.540087 | 3.1:+80006317 | None:intergenic |
| GTGGAGTTACGGAGTGGTGG+TGG | 0.542600 | 3.1:+80006263 | None:intergenic |
| AGAAGAAGGAACACTGGAAG+CGG | 0.543572 | 3.1:-80006437 | MS.gene48438:CDS |
| GTGCTGCTTGTAACTGTCAC+CGG | 0.544839 | 3.1:-80006408 | MS.gene48438:CDS |
| CAAAAGTGGGCCAGCAGGAA+CGG | 0.546267 | 3.1:-80006545 | MS.gene48438:CDS |
| GGGGTTCCCTGTTACGCCGG+TGG | 0.551765 | 3.1:-80006662 | MS.gene48438:CDS |
| GGAGGAGCAGCCACAGGCGG+TGG | 0.554790 | 3.1:-80006571 | MS.gene48438:CDS |
| GTGGCATGTTACGACTCGCT+TGG | 0.557648 | 3.1:-80006643 | MS.gene48438:CDS |
| AAGAATCACGCCGTCAGCAT+AGG | 0.558884 | 3.1:-80006502 | MS.gene48438:CDS |
| ACGGAGTGGTGGTGGAAACC+AGG | 0.562207 | 3.1:+80006271 | None:intergenic |
| AATCCCCTACTCGATGCAGC+TGG | 0.564604 | 3.1:+80006208 | None:intergenic |
| GCGGACGGAGGAGCAGCCAC+AGG | 0.565208 | 3.1:-80006577 | MS.gene48438:CDS |
| TACGACTCGCTTGGAAACTC+AGG | 0.569322 | 3.1:-80006634 | MS.gene48438:CDS |
| AATCACGCCGTCAGCATAGG+TGG | 0.574956 | 3.1:-80006499 | MS.gene48438:CDS |
| ATAGGTGGTCACGCCGTGGA+TGG | 0.576462 | 3.1:-80006484 | MS.gene48438:CDS |
| CCCCTACTCGATGCAGCTGG+TGG | 0.577449 | 3.1:+80006211 | None:intergenic |
| CAGGCGGTGGTGTCAAAAGT+GGG | 0.580393 | 3.1:-80006558 | MS.gene48438:CDS |
| AATTGGGTGGAGAATTCAGA+AGG | 0.584858 | 3.1:-80006044 | MS.gene48438:CDS |
| AACATGCCACCGGCGTAACA+GGG | 0.586523 | 3.1:+80006656 | None:intergenic |
| TCAAATCCAAGCAGCAGCGG+TGG | 0.591349 | 3.1:-80006163 | MS.gene48438:CDS |
| AGCGAGTCGTAACATGCCAC+CGG | 0.591718 | 3.1:+80006646 | None:intergenic |
| AGAATTCAGAAGGAAGATGA+AGG | 0.605493 | 3.1:-80006034 | MS.gene48438:CDS |
| GCAGCTGGTGGAAGAGCCAA+CGG | 0.605589 | 3.1:+80006223 | None:intergenic |
| GACGGAGGAGCAGCCACAGG+CGG | 0.606719 | 3.1:-80006574 | MS.gene48438:CDS |
| CATTTGCGGAGAAAATTGGG+TGG | 0.607411 | 3.1:-80006057 | MS.gene48438:CDS |
| GCGGAGGAGGTAACGCTGCA+CGG | 0.609223 | 3.1:+80006293 | None:intergenic |
| TCGGTGGAGTTACGGAGTGG+TGG | 0.614261 | 3.1:+80006260 | None:intergenic |
| GGCGTGACCACCTATGCTGA+CGG | 0.619665 | 3.1:+80006492 | None:intergenic |
| CCACCAGCTGCATCGAGTAG+GGG | 0.624461 | 3.1:-80006211 | MS.gene48438:CDS |
| TTCCACCAGCTGCATCGAGT+AGG | 0.625338 | 3.1:-80006213 | MS.gene48438:CDS |
| CAGCATAGGTGGTCACGCCG+TGG | 0.632841 | 3.1:-80006488 | MS.gene48438:CDS |
| TGGCGGTGGAATCGGTTCAG+CGG | 0.633285 | 3.1:-80006596 | MS.gene48438:CDS |
| GATATCACAACATCGTCCGT+TGG | 0.638979 | 3.1:-80006239 | MS.gene48438:CDS |
| TGCCGGAGAAGAAGGAACAC+TGG | 0.644421 | 3.1:-80006443 | MS.gene48438:CDS |
| GGACGATGTTGTGATATCGG+TGG | 0.659157 | 3.1:+80006244 | None:intergenic |
| AGTAAGGTGATAATTGATGG+TGG | 0.668303 | 3.1:+80006320 | None:intergenic |
| AAGGTGATAATTGATGGTGG+TGG | 0.673505 | 3.1:+80006323 | None:intergenic |
| GAGTGGTGGTGGAAACCAGG+CGG | 0.674377 | 3.1:+80006274 | None:intergenic |
| GGAATCGGTTCAGCGGACGG+AGG | 0.678169 | 3.1:-80006589 | MS.gene48438:CDS |
| GTTTAGAACAAAATTCACAC+AGG | 0.682835 | 3.1:-80006101 | MS.gene48438:CDS |
| AAACTCGCAACATCCATCCA+CGG | 0.687116 | 3.1:+80006471 | None:intergenic |
| GTGTCAAATCCAAGCAGCAG+CGG | 0.696717 | 3.1:-80006166 | MS.gene48438:CDS |
| TTTCACCGCAAAGAAATCGA+CGG | 0.699703 | 3.1:-80006382 | MS.gene48438:CDS |
| ATATCGGTGGAGTTACGGAG+TGG | 0.773672 | 3.1:+80006257 | None:intergenic |
| ACGGTGAATTCACTCCCCAG+CGG | 0.785774 | 3.1:-80006363 | MS.gene48438:CDS |
| GAGGAGGTAACGCTGCACGG+TGG | 0.795380 | 3.1:+80006296 | None:intergenic |
| TGAGGGTGATGAGACCGCTG+GGG | 0.809094 | 3.1:+80006349 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATTCACACAGGAACAAA+AGG | - | chr3.1:80006509-80006528 | MS.gene48438:CDS | 30.0% | |
| ! | GTTTAGAACAAAATTCACAC+AGG | - | chr3.1:80006500-80006519 | MS.gene48438:CDS | 30.0% |
| AGAATTCAGAAGGAAGATGA+AGG | - | chr3.1:80006567-80006586 | MS.gene48438:CDS | 35.0% | |
| AGTAAGGTGATAATTGATGG+TGG | + | chr3.1:80006284-80006303 | None:intergenic | 35.0% | |
| GGTAGTAAGGTGATAATTGA+TGG | + | chr3.1:80006287-80006306 | None:intergenic | 35.0% | |
| TATCAAGAGACATGTGCTTA+AGG | - | chr3.1:80006620-80006639 | MS.gene48438:CDS | 35.0% | |
| TTGCATTTGCGGAGAAAATT+GGG | - | chr3.1:80006541-80006560 | MS.gene48438:CDS | 35.0% | |
| AACGGACGATGTTGTGATAT+CGG | + | chr3.1:80006363-80006382 | None:intergenic | 40.0% | |
| AATTGGGTGGAGAATTCAGA+AGG | - | chr3.1:80006557-80006576 | MS.gene48438:CDS | 40.0% | |
| AGAGACATGTGCTTAAGGTT+TGG | - | chr3.1:80006625-80006644 | MS.gene48438:CDS | 40.0% | |
| CACAATAACAAGCACACTCT+TGG | - | chr3.1:80006651-80006670 | MS.gene48438:CDS | 40.0% | |
| CTTGCATTTGCGGAGAAAAT+TGG | - | chr3.1:80006540-80006559 | MS.gene48438:CDS | 40.0% | |
| GGATAAGATGCTTGCATTTG+CGG | - | chr3.1:80006530-80006549 | MS.gene48438:CDS | 40.0% | |
| TCAGGAGTTAGGTCCAAAAA+TGG | - | chr3.1:80005985-80006004 | MS.gene48438:CDS | 40.0% | |
| TTGTGATATCGGTGGAGTTA+CGG | + | chr3.1:80006352-80006371 | None:intergenic | 40.0% | |
| TTTCACCGCAAAGAAATCGA+CGG | - | chr3.1:80006219-80006238 | MS.gene48438:CDS | 40.0% | |
| TTTCTTTGCGGTGAAAGTTC+CGG | + | chr3.1:80006215-80006234 | None:intergenic | 40.0% | |
| !! | AAGGTGATAATTGATGGTGG+TGG | + | chr3.1:80006281-80006300 | None:intergenic | 40.0% |
| !! | ATTCACCGTCGATTTCTTTG+CGG | + | chr3.1:80006227-80006246 | None:intergenic | 40.0% |
| AAACTCGCAACATCCATCCA+CGG | + | chr3.1:80006133-80006152 | None:intergenic | 45.0% | |
| AGAAGAAGGAACACTGGAAG+CGG | - | chr3.1:80006164-80006183 | MS.gene48438:CDS | 45.0% | |
| CATTTGCGGAGAAAATTGGG+TGG | - | chr3.1:80006544-80006563 | MS.gene48438:CDS | 45.0% | |
| GAGGAAGTGGAACGAAGAAA+AGG | - | chr3.1:80006478-80006497 | MS.gene48438:CDS | 45.0% | |
| GATATCACAACATCGTCCGT+TGG | - | chr3.1:80006362-80006381 | MS.gene48438:CDS | 45.0% | |
| GGAGTTAGGTCCAAAAATGG+CGG | - | chr3.1:80005988-80006007 | MS.gene48438:CDS | 45.0% | |
| TCCAAAAATGGCGGTGGAAT+CGG | - | chr3.1:80005997-80006016 | MS.gene48438:CDS | 45.0% | |
| TTCCAGTGTTCCTTCTTCTC+CGG | + | chr3.1:80006163-80006182 | None:intergenic | 45.0% | |
| !! | AATTGATGGTGGTGGTGTTG+AGG | + | chr3.1:80006273-80006292 | None:intergenic | 45.0% |
| !! | ATTGATGGTGGTGGTGTTGA+GGG | + | chr3.1:80006272-80006291 | None:intergenic | 45.0% |
| !!! | TGTTGCGAGTTTTTAGCTGC+CGG | - | chr3.1:80006141-80006160 | MS.gene48438:CDS | 45.0% |
| !!! | TTTTTAGCTGCCGGAGAAGA+AGG | - | chr3.1:80006150-80006169 | MS.gene48438:CDS | 45.0% |
| AAGAATCACGCCGTCAGCAT+AGG | - | chr3.1:80006099-80006118 | MS.gene48438:CDS | 50.0% | |
| ATATCGGTGGAGTTACGGAG+TGG | + | chr3.1:80006347-80006366 | None:intergenic | 50.0% | |
| CGCTTGGAAACTCAGGAGTT+AGG | - | chr3.1:80005974-80005993 | MS.gene48438:CDS | 50.0% | |
| GGACGATGTTGTGATATCGG+TGG | + | chr3.1:80006360-80006379 | None:intergenic | 50.0% | |
| GTGTCAAATCCAAGCAGCAG+CGG | - | chr3.1:80006435-80006454 | MS.gene48438:CDS | 50.0% | |
| GTTAGGTCCAAAAATGGCGG+TGG | - | chr3.1:80005991-80006010 | MS.gene48438:CDS | 50.0% | |
| TACGACTCGCTTGGAAACTC+AGG | - | chr3.1:80005967-80005986 | MS.gene48438:CDS | 50.0% | |
| TTGTATCTGACCGTTCCTGC+TGG | + | chr3.1:80006069-80006088 | None:intergenic | 50.0% | |
| !! | ACCGATTCCACCGCCATTTT+TGG | + | chr3.1:80006001-80006020 | None:intergenic | 50.0% |
| !! | GTGCTGCTTGTAACTGTCAC+CGG | - | chr3.1:80006193-80006212 | MS.gene48438:CDS | 50.0% |
| AACATGCCACCGGCGTAACA+GGG | + | chr3.1:80005948-80005967 | None:intergenic | 55.0% | |
| AACGCTGCACGGTGGTAGTA+AGG | + | chr3.1:80006300-80006319 | None:intergenic | 55.0% | |
| AATCACGCCGTCAGCATAGG+TGG | - | chr3.1:80006102-80006121 | MS.gene48438:CDS | 55.0% | |
| AATCCCCTACTCGATGCAGC+TGG | + | chr3.1:80006396-80006415 | None:intergenic | 55.0% | |
| ACGGTGAATTCACTCCCCAG+CGG | - | chr3.1:80006238-80006257 | MS.gene48438:CDS | 55.0% | |
| AGCGAGTCGTAACATGCCAC+CGG | + | chr3.1:80005958-80005977 | None:intergenic | 55.0% | |
| CAAAAGTGGGCCAGCAGGAA+CGG | - | chr3.1:80006056-80006075 | MS.gene48438:CDS | 55.0% | |
| GTGGCATGTTACGACTCGCT+TGG | - | chr3.1:80005958-80005977 | MS.gene48438:CDS | 55.0% | |
| TAACATGCCACCGGCGTAAC+AGG | + | chr3.1:80005949-80005968 | None:intergenic | 55.0% | |
| TCAAATCCAAGCAGCAGCGG+TGG | - | chr3.1:80006438-80006457 | MS.gene48438:CDS | 55.0% | |
| TCCACCAGCTGCATCGAGTA+GGG | - | chr3.1:80006389-80006408 | MS.gene48438:CDS | 55.0% | |
| TGCCGGAGAAGAAGGAACAC+TGG | - | chr3.1:80006158-80006177 | MS.gene48438:CDS | 55.0% | |
| TTCCACCAGCTGCATCGAGT+AGG | - | chr3.1:80006388-80006407 | MS.gene48438:CDS | 55.0% | |
| TTGAGGGTGATGAGACCGCT+GGG | + | chr3.1:80006256-80006275 | None:intergenic | 55.0% | |
| ! | ACAGGCGGTGGTGTCAAAAG+TGG | - | chr3.1:80006042-80006061 | MS.gene48438:CDS | 55.0% |
| ! | ACTTTTGACACCACCGCCTG+TGG | + | chr3.1:80006043-80006062 | None:intergenic | 55.0% |
| ! | CAGGCGGTGGTGTCAAAAGT+GGG | - | chr3.1:80006043-80006062 | MS.gene48438:CDS | 55.0% |
| AATCCAAGCAGCAGCGGTGG+TGG | - | chr3.1:80006441-80006460 | MS.gene48438:CDS | 60.0% | |
| ATAGGTGGTCACGCCGTGGA+TGG | - | chr3.1:80006117-80006136 | MS.gene48438:CDS | 60.0% | |
| CCACCAGCTGCATCGAGTAG+GGG | - | chr3.1:80006390-80006409 | MS.gene48438:CDS | 60.0% | |
| GCAGCTGGTGGAAGAGCCAA+CGG | + | chr3.1:80006381-80006400 | None:intergenic | 60.0% | |
| GGCGTGACCACCTATGCTGA+CGG | + | chr3.1:80006112-80006131 | None:intergenic | 60.0% | |
| GGTGTCAAAAGTGGGCCAGC+AGG | - | chr3.1:80006051-80006070 | MS.gene48438:CDS | 60.0% | |
| GTGGAGTTACGGAGTGGTGG+TGG | + | chr3.1:80006341-80006360 | None:intergenic | 60.0% | |
| GTTGAGGGTGATGAGACCGC+TGG | + | chr3.1:80006257-80006276 | None:intergenic | 60.0% | |
| TCGGTGGAGTTACGGAGTGG+TGG | + | chr3.1:80006344-80006363 | None:intergenic | 60.0% | |
| TGAGGGTGATGAGACCGCTG+GGG | + | chr3.1:80006255-80006274 | None:intergenic | 60.0% | |
| ! | GGTGGAATCGGTTCAGCGGA+CGG | - | chr3.1:80006009-80006028 | MS.gene48438:CDS | 60.0% |
| ! | TGGCGGTGGAATCGGTTCAG+CGG | - | chr3.1:80006005-80006024 | MS.gene48438:CDS | 60.0% |
| !! | ACGGAGTGGTGGTGGAAACC+AGG | + | chr3.1:80006333-80006352 | None:intergenic | 60.0% |
| !! | GAGTGGTGGTGGAAACCAGG+CGG | + | chr3.1:80006330-80006349 | None:intergenic | 60.0% |
| !! | GGTGGTAGTGGAGGAGGAAG+TGG | - | chr3.1:80006465-80006484 | MS.gene48438:CDS | 60.0% |
| CAGCATAGGTGGTCACGCCG+TGG | - | chr3.1:80006113-80006132 | MS.gene48438:CDS | 65.0% | |
| CCCCTACTCGATGCAGCTGG+TGG | + | chr3.1:80006393-80006412 | None:intergenic | 65.0% | |
| GAGGAGGTAACGCTGCACGG+TGG | + | chr3.1:80006308-80006327 | None:intergenic | 65.0% | |
| GCGGAGGAGGTAACGCTGCA+CGG | + | chr3.1:80006311-80006330 | None:intergenic | 65.0% | |
| TGGTGGAAACCAGGCGGAGG+AGG | + | chr3.1:80006324-80006343 | None:intergenic | 65.0% | |
| ! | GGAATCGGTTCAGCGGACGG+AGG | - | chr3.1:80006012-80006031 | MS.gene48438:CDS | 65.0% |
| !! | AGCGGTGGTGGTGGTGGTAG+TGG | - | chr3.1:80006453-80006472 | MS.gene48438:CDS | 65.0% |
| !! | GGTGGTGGTGGTAGTGGAGG+AGG | - | chr3.1:80006459-80006478 | MS.gene48438:CDS | 65.0% |
| !! | GGTGGTGGTGGTGGTAGTGG+AGG | - | chr3.1:80006456-80006475 | MS.gene48438:CDS | 65.0% |
| !! | TGGTGGTGGAAACCAGGCGG+AGG | + | chr3.1:80006327-80006346 | None:intergenic | 65.0% |
| CCACCACCACCGCTGCTGCT+TGG | + | chr3.1:80006447-80006466 | None:intergenic | 70.0% | |
| GACGGAGGAGCAGCCACAGG+CGG | - | chr3.1:80006027-80006046 | MS.gene48438:CDS | 70.0% | |
| GCAGCGTTACCTCCTCCGCC+TGG | - | chr3.1:80006312-80006331 | MS.gene48438:CDS | 70.0% | |
| GGGGTTCCCTGTTACGCCGG+TGG | - | chr3.1:80005939-80005958 | MS.gene48438:CDS | 70.0% | |
| ! | CCAAGCAGCAGCGGTGGTGG+TGG | - | chr3.1:80006444-80006463 | MS.gene48438:CDS | 70.0% |
| !! | AGCAGCAGCGGTGGTGGTGG+TGG | - | chr3.1:80006447-80006466 | MS.gene48438:CDS | 70.0% |
| GCGGACGGAGGAGCAGCCAC+AGG | - | chr3.1:80006024-80006043 | MS.gene48438:CDS | 75.0% | |
| GGAGGAGCAGCCACAGGCGG+TGG | - | chr3.1:80006030-80006049 | MS.gene48438:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 80005937 | 80006686 | 80005937 | ID=MS.gene48438 |
| chr3.1 | mRNA | 80005937 | 80006686 | 80005937 | ID=MS.gene48438.t1;Parent=MS.gene48438 |
| chr3.1 | exon | 80005937 | 80006686 | 80005937 | ID=MS.gene48438.t1.exon1;Parent=MS.gene48438.t1 |
| chr3.1 | CDS | 80005937 | 80006686 | 80005937 | ID=cds.MS.gene48438.t1;Parent=MS.gene48438.t1 |
| Gene Sequence |
| Protein sequence |
