AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65317

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65317.t1 MTR_5g024020 98.675 755 10 0 1 755 1 755 0.0 1546
MS.gene65317.t1 MTR_5g024020 94.040 755 9 1 1 755 1 719 0.0 1461
MS.gene65317.t1 MTR_8g018730 83.179 755 124 2 1 755 1 752 0.0 1328
MS.gene65317.t1 MTR_8g018735 83.974 755 119 2 1 755 1 753 0.0 1325
MS.gene65317.t1 MTR_8g018730 79.470 755 122 3 1 755 1 722 0.0 1256
MS.gene65317.t1 MTR_2g099560 71.788 755 201 7 3 755 7 751 0.0 1139
MS.gene65317.t1 MTR_2g099570 69.272 755 218 6 3 755 7 749 0.0 1082
MS.gene65317.t1 MTR_8g018420 68.499 746 222 5 12 755 9 743 0.0 1078
MS.gene65317.t1 MTR_3g081130 67.852 759 227 9 1 755 1 746 0.0 1063
MS.gene65317.t1 MTR_8g018735 82.496 617 106 2 1 617 1 615 0.0 1052
MS.gene65317.t1 MTR_8g018570 64.069 757 262 7 3 755 7 757 0.0 1030
MS.gene65317.t1 MTR_8g018550 65.873 756 248 7 3 755 7 755 0.0 1024
MS.gene65317.t1 MTR_8g018430 64.486 749 256 6 9 755 8 748 0.0 1023
MS.gene65317.t1 MTR_8g018690 63.576 755 264 5 3 755 7 752 0.0 1016
MS.gene65317.t1 MTR_8g018510 64.619 749 256 7 11 755 13 756 0.0 1013
MS.gene65317.t1 MTR_8g020990 63.841 755 265 6 3 755 7 755 0.0 1009
MS.gene65317.t1 MTR_8g018520 63.757 756 264 7 3 755 7 755 0.0 1001
MS.gene65317.t1 MTR_3g081130 69.617 678 197 6 82 755 2 674 0.0 996
MS.gene65317.t1 MTR_8g018690 61.457 755 260 6 3 755 7 732 0.0 969
MS.gene65317.t1 MTR_8g018450 59.121 751 258 11 10 755 9 715 0.0 903
MS.gene65317.t1 MTR_1g083020 56.955 762 289 10 6 755 12 746 0.0 878
MS.gene65317.t1 MTR_8g018730 86.624 471 63 0 285 755 2 472 0.0 864
MS.gene65317.t1 MTR_1g104650 55.425 765 301 12 5 755 1 739 0.0 852
MS.gene65317.t1 MTR_7g113410 55.297 774 301 13 1 755 8 755 0.0 851
MS.gene65317.t1 MTR_8g018650 54.618 747 275 12 12 755 9 694 0.0 799
MS.gene65317.t1 MTR_8g018620 54.473 749 273 14 12 755 9 694 0.0 791
MS.gene65317.t1 MTR_8g018590 55.571 709 264 13 65 755 35 710 0.0 768
MS.gene65317.t1 MTR_8g020970 47.034 708 354 12 57 752 113 811 0.0 642
MS.gene65317.t1 MTR_4g066170 43.553 760 386 18 4 745 48 782 0.0 592
MS.gene65317.t1 MTR_3g479460 42.550 745 388 15 18 745 58 779 0.0 588
MS.gene65317.t1 MTR_8g011340 43.508 724 370 17 56 755 46 754 0.0 578
MS.gene65317.t1 MTR_8g080230 43.269 728 374 18 50 755 92 802 0.0 574
MS.gene65317.t1 MTR_3g079450 40.595 739 400 14 24 745 62 778 0.0 560
MS.gene65317.t1 MTR_3g079420 45.286 647 329 11 113 745 106 741 0.0 543
MS.gene65317.t1 MTR_4g088810 43.333 660 332 14 104 752 151 779 5.88e-180 539
MS.gene65317.t1 MTR_8g020970 44.852 573 295 12 57 617 113 676 5.54e-154 465
MS.gene65317.t1 MTR_8g021020 65.636 291 96 1 469 755 28 318 1.53e-136 411
MS.gene65317.t1 MTR_3g079450 37.643 611 342 14 24 617 62 650 4.21e-128 397
MS.gene65317.t1 MTR_6g452500 42.969 512 264 13 254 754 14 508 2.88e-127 394
MS.gene65317.t1 MTR_7g012030 60.684 117 45 1 522 637 87 203 1.03e-40 149
MS.gene65317.t1 MTR_6g046330 54.688 128 46 2 522 637 77 204 1.60e-35 137
MS.gene65317.t1 MTR_8g463810 37.611 226 74 5 430 653 197 357 1.96e-34 137
MS.gene65317.t1 MTR_5g023990 66.176 68 10 1 653 720 145 199 2.99e-20 92.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65317.t1 AT1G55020 56.447 760 287 12 10 755 18 747 0.0 858
MS.gene65317.t1 AT3G22400 53.796 764 309 12 10 755 32 769 0.0 822
MS.gene65317.t1 AT1G17420 45.827 707 361 12 57 752 108 803 0.0 617
MS.gene65317.t1 AT1G72520 45.545 707 364 11 57 752 114 810 0.0 615
MS.gene65317.t1 AT3G45140 44.932 730 361 20 30 745 70 772 0.0 585
MS.gene65317.t1 AT3G45140 48.287 642 303 16 118 745 4 630 0.0 578
MS.gene65317.t1 AT1G67560 44.648 710 354 15 64 753 111 801 0.0 562

Find 178 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCATGCAGCTGTTAATTT+TGG 0.118332 5.2:+14912950 MS.gene65317:CDS
TTTGATTGGGATGCTGATTT+TGG 0.190953 5.2:+14909373 MS.gene65317:CDS
TTGAATTTGTTTGCAATTCA+TGG 0.215771 5.2:+14909491 MS.gene65317:CDS
TTCACCATCTCATCATAATT+TGG 0.225540 5.2:-14913046 None:intergenic
CCACAGTGCCCTTGATCTTT+TGG 0.235465 5.2:-14906119 None:intergenic
CATTTACCATTCACTTTGAT+TGG 0.244250 5.2:+14909359 MS.gene65317:CDS
TTACAAGCCTTTCAAAAGTT+TGG 0.259729 5.2:+14913220 MS.gene65317:CDS
TGGTCGAAAACCAACTAAAA+AGG 0.263615 5.2:+14909932 MS.gene65317:CDS
TCTTGAATATTCTGCATCTT+AGG 0.264727 5.2:-14912884 None:intergenic
GGATCTAGCAACTTGTTAAT+AGG 0.291816 5.2:-14912294 None:intergenic
GAGAGAGGGATGTTAAGTTT+TGG 0.326208 5.2:+14913179 MS.gene65317:intron
ATTTATTGTGTCACGGAAAT+GGG 0.326994 5.2:-14912316 None:intergenic
AGATGTTTGGCATATTTGAT+AGG 0.330603 5.2:+14906088 None:intergenic
CTTATGGAACAGGCACTTAA+TGG 0.333183 5.2:+14911744 MS.gene65317:intron
GGATTTGGAGCTTACATCAT+TGG 0.341118 5.2:-14909557 None:intergenic
TATTTATTGTGTCACGGAAA+TGG 0.341127 5.2:-14912317 None:intergenic
CAGTTGACGGACTAGAGATA+TGG 0.343179 5.2:+14912717 MS.gene65317:CDS
AATTCAACGGATTCACGATT+AGG 0.343202 5.2:-14909475 None:intergenic
TCTTGTCTGTAGAAGACTAA+TGG 0.350358 5.2:-14909741 None:intergenic
CATCAGCAGCATACAAGAAC+TGG 0.362677 5.2:+14912427 MS.gene65317:CDS
GTTCAATACCACCTTCAAAT+AGG 0.363395 5.2:-14910261 None:intergenic
ATTTGAAACTTACTTGTAGA+AGG 0.370231 5.2:-14911251 None:intergenic
CATCCAAACGGTGTTAAGTA+TGG 0.373347 5.2:+14911918 MS.gene65317:CDS
ACGTTAGATATTGCTGTCTA+TGG 0.376114 5.2:+14911554 MS.gene65317:CDS
TGATAGTACAATTTGGCTAT+TGG 0.379351 5.2:+14911980 MS.gene65317:CDS
TGGATGACTGACGAAGAATT+TGG 0.388201 5.2:+14911189 MS.gene65317:CDS
TCTAGTCCGTCAACTGCATA+AGG 0.389360 5.2:-14912710 None:intergenic
TCGAAGCTATCAAACTCATT+TGG 0.389705 5.2:-14910226 None:intergenic
GCACCATACTTAACACCGTT+TGG 0.397039 5.2:-14911921 None:intergenic
CAGTCCCTTACCAGTCCTTA+AGG 0.399613 5.2:+14910309 MS.gene65317:CDS
GATCTAGCAACTTGTTAATA+GGG 0.403902 5.2:-14912293 None:intergenic
GACGGTGCTACTGCCTTCTT+AGG 0.411023 5.2:+14906261 MS.gene65317:CDS
TCATCTGACTTCCTTACATA+TGG 0.412674 5.2:+14910136 MS.gene65317:CDS
GATGACATCTCAACAGAATT+CGG 0.416030 5.2:-14912408 None:intergenic
GGCAGCATCAAGAATGTTCC+CGG 0.417139 5.2:-14906214 None:intergenic
TGGAAGGAAGTTGTAGAAAA+AGG 0.421210 5.2:+14912830 MS.gene65317:CDS
ATGTGGTAAACTCAACTCTA+TGG 0.424161 5.2:-14911898 None:intergenic
TAATTTGGAGTTCCTTTCTC+CGG 0.424665 5.2:-14913031 None:intergenic
TGTCCTAGTGGCATAAGCTT+TGG 0.431901 5.2:-14911835 None:intergenic
AAGGTGTTGATAGTACAATT+TGG 0.435084 5.2:+14911973 MS.gene65317:CDS
CTGTTTGTTGCTATGATGAA+TGG 0.436847 5.2:-14912252 None:intergenic
AAGCTTGCTCGTCCAGTCCT+TGG 0.441085 5.2:+14909870 MS.gene65317:CDS
AAGAAGACCACCTTGTTTGA+CGG 0.441487 5.2:-14906184 None:intergenic
ATATGATAATGGAACATGAT+TGG 0.444515 5.2:-14912913 None:intergenic
GTTCCAAGAGATGAAAACTT+TGG 0.447882 5.2:+14910103 MS.gene65317:CDS
ATTTACCATTCACTTTGATT+GGG 0.449296 5.2:+14909360 MS.gene65317:CDS
CAGTGCGGAAAATTTCCTTA+AGG 0.449942 5.2:-14910324 None:intergenic
CTATCAACACCTTGGTCTGC+AGG 0.450678 5.2:-14911963 None:intergenic
CTGCCGGCAGCTTGGTCGGC+GGG 0.451570 5.2:+14906232 MS.gene65317:CDS
GCAAATGGCAAGGGATTAGT+TGG 0.452072 5.2:+14909274 MS.gene65317:intron
AATCATGTTCCATTATCATA+TGG 0.452609 5.2:+14912915 MS.gene65317:CDS
GCTCCCAAGGTTGGTAGAGT+GGG 0.452917 5.2:-14909325 None:intergenic
TTCCTTATGTAAAATGCTCC+TGG 0.457180 5.2:-14909400 None:intergenic
TCTTGATGCTGCCGGCAGCT+TGG 0.457862 5.2:+14906224 MS.gene65317:CDS
GAGCAGCTGGAGATTAATCT+AGG 0.461032 5.2:+14911602 MS.gene65317:CDS
TGCATCTTGTCTTGCTCCCA+AGG 0.467682 5.2:-14909338 None:intergenic
GCAAGCTTGACATCTTCATC+TGG 0.469750 5.2:-14909855 None:intergenic
TTTGGAAGAGAGATGGTTGC+TGG 0.470580 5.2:+14911207 MS.gene65317:CDS
CTTGCTCGTCCAGTCCTTGG+AGG 0.472483 5.2:+14909873 MS.gene65317:CDS
GGTGGTCTTCTTGATGCTGC+CGG 0.474897 5.2:+14906195 MS.gene65317:CDS
CTTCTAACCCTTCGAGGGTA+AGG 0.480584 5.2:-14909909 None:intergenic
GATGTTTGGCATATTTGATA+GGG 0.480663 5.2:+14906089 None:intergenic
AATTTCCTTAAGGACTGGTA+AGG 0.482665 5.2:-14910314 None:intergenic
TCTTGTCTTGCTCCCAAGGT+TGG 0.483250 5.2:-14909334 None:intergenic
AGAAGCAGTCCATATGATAA+TGG 0.483627 5.2:-14912924 None:intergenic
TCAAAAGTTTGGAAGTAAGC+TGG 0.485045 5.2:+14913231 MS.gene65317:CDS
CGACCACTTCTAACCCTTCG+AGG 0.485068 5.2:-14909915 None:intergenic
CGAAGATGGATCCCGGAGAA+AGG 0.485557 5.2:+14913019 MS.gene65317:CDS
GGGAACATTCTTGATGCTGC+CGG 0.486249 5.2:+14906216 MS.gene65317:CDS
ACCAGTGGCTCATGAGTTGA+TGG 0.487070 5.2:-14912037 None:intergenic
TACTGGACAACGAAAAGAAT+GGG 0.494054 5.2:+14909791 MS.gene65317:CDS
TACAATTTGGCTATTGGCCA+AGG 0.495798 5.2:+14911986 MS.gene65317:CDS
TGCTCCCAAGGTTGGTAGAG+TGG 0.496348 5.2:-14909326 None:intergenic
CGGAAAATTTCCTTAAGGAC+TGG 0.497528 5.2:-14910319 None:intergenic
GTGGTCTTCTTGATGCTGCC+GGG 0.498077 5.2:+14906196 MS.gene65317:CDS
AATGCTCTAATTAATGCTGA+TGG 0.498741 5.2:+14912357 MS.gene65317:CDS
TATGATGTCTACAATGATCT+TGG 0.499215 5.2:+14909828 MS.gene65317:CDS
GTCCCCACTCTACCAACCTT+GGG 0.499236 5.2:+14909322 MS.gene65317:CDS
AGGTGGGCTCACAGTAGAAA+AGG 0.500073 5.2:+14911622 MS.gene65317:CDS
AATCTAAGAGGAGATGGTAC+TGG 0.504050 5.2:+14909774 MS.gene65317:CDS
GGATCATAGCCAAAAGATCA+AGG 0.507959 5.2:+14906110 MS.gene65317:CDS
GAAGAATTGCAAAATCTAAG+AGG 0.508305 5.2:+14909762 MS.gene65317:CDS
GTGCTAGACCCTCCAAGGAC+TGG 0.510390 5.2:-14909882 None:intergenic
TACTTAACACCGTTTGGATG+TGG 0.510600 5.2:-14911915 None:intergenic
AATTTGGAGTTCCTTTCTCC+GGG 0.512509 5.2:-14913030 None:intergenic
CGTTGATATTTATTGTGTCA+CGG 0.512648 5.2:-14912323 None:intergenic
AGTAAGCTGGCCAAAATTGA+AGG 0.513964 5.2:+14913244 MS.gene65317:CDS
ATTTACAATTACATGAGCCT+TGG 0.514004 5.2:-14912003 None:intergenic
AGCATCCCAATCAAAGTGAA+TGG 0.517285 5.2:-14909365 None:intergenic
GATGCTGCCGGCAGCTTGGT+CGG 0.520219 5.2:+14906228 MS.gene65317:CDS
CTAGTCCGTCAACTGCATAA+GGG 0.521062 5.2:-14912709 None:intergenic
ATCTCAACAGAATTCGGCCC+AGG 0.524407 5.2:-14912402 None:intergenic
AATGGAGCTGGTGTTTGACT+TGG 0.524882 5.2:-14909723 None:intergenic
TGTCCCCACTCTACCAACCT+TGG 0.528094 5.2:+14909321 MS.gene65317:CDS
CTCATCAGTTGGATAATACA+AGG 0.529376 5.2:-14912774 None:intergenic
GTCCGACACTTAGTCGAAGA+TGG 0.531477 5.2:+14913005 MS.gene65317:CDS
TTCAGAATATAACCTCCATA+AGG 0.532169 5.2:-14912980 None:intergenic
GACTACCCTTATGCAGTTGA+CGG 0.533613 5.2:+14912704 MS.gene65317:CDS
CGAAGAATTTGGAAGAGAGA+TGG 0.533979 5.2:+14911200 MS.gene65317:CDS
GTGCTACTGCCTTCTTAGGC+CGG 0.535421 5.2:+14906265 MS.gene65317:CDS
AGATACGTTCCGGCCTAAGA+AGG 0.535886 5.2:-14906274 None:intergenic
ACCATCAACTCATGAGCCAC+TGG 0.540362 5.2:+14912036 MS.gene65317:CDS
AGTTGACGGACTAGAGATAT+GGG 0.546670 5.2:+14912718 MS.gene65317:CDS
TAAGAAAAGGATTGTCCTAG+TGG 0.548685 5.2:-14911847 None:intergenic
GACCACTTCTAACCCTTCGA+GGG 0.549674 5.2:-14909914 None:intergenic
TATCCCGCCGACCAAGCTGC+CGG 0.549835 5.2:-14906235 None:intergenic
TTGCAAAATCTAAGAGGAGA+TGG 0.549962 5.2:+14909768 MS.gene65317:CDS
ACACTGAATTACAAACTTGG+TGG 0.550213 5.2:+14912810 MS.gene65317:CDS
TACCTCTTGATAAGATCAGT+TGG 0.553053 5.2:-14912471 None:intergenic
AACTCCAAATTATGATGAGA+TGG 0.555053 5.2:+14913042 MS.gene65317:CDS
TTGGTCGGCGGGATAATTGA+CGG 0.557397 5.2:+14906243 MS.gene65317:CDS
ATGACACTGAATTACAAACT+TGG 0.557584 5.2:+14912807 MS.gene65317:CDS
TGAATTGCAAACAAATTCAA+CGG 0.559629 5.2:-14909488 None:intergenic
ATGGGATGCTATTAAGTCAT+GGG 0.562373 5.2:+14912736 MS.gene65317:CDS
AGCTGGAGATTAATCTAGGT+GGG 0.562396 5.2:+14911606 MS.gene65317:CDS
TGGCCTTCGTCTTGTGATCG+AGG 0.568239 5.2:+14912682 MS.gene65317:CDS
TTGCTCGTCCAGTCCTTGGA+GGG 0.571582 5.2:+14909874 MS.gene65317:CDS
AGATGACAAACCATCCGTCT+TGG 0.572064 5.2:-14906316 None:intergenic
ATTTCCTTAAGGACTGGTAA+GGG 0.573930 5.2:-14910313 None:intergenic
ATGCCAAAGCTTATGCCACT+AGG 0.574343 5.2:+14911832 MS.gene65317:CDS
AAGATTACAGATTTAAACAA+AGG 0.575473 5.2:-14910187 None:intergenic
GCTGCCGGCAGCTTGGTCGG+CGG 0.581708 5.2:+14906231 MS.gene65317:CDS
AAACTCAACTCTATGGCCAC+TGG 0.582518 5.2:-14911891 None:intergenic
TCAACCGCATAGATACGTTC+CGG 0.583360 5.2:-14906284 None:intergenic
TTGAGTTTACCACATCCAAA+CGG 0.584875 5.2:+14911906 MS.gene65317:CDS
ATCAGTGCTACCAAGACGGA+TGG 0.585905 5.2:+14906306 MS.gene65317:CDS
GCACTCATCCTTACCCTCGA+AGG 0.587455 5.2:+14909901 MS.gene65317:CDS
GCTGTAACGTCCGTCAAACA+AGG 0.587930 5.2:+14906174 MS.gene65317:CDS
TTCTGCATCTTAGGCCACCA+TGG 0.587934 5.2:-14912875 None:intergenic
TATGGGATGCTATTAAGTCA+TGG 0.588056 5.2:+14912735 MS.gene65317:CDS
ATCATCTTGCCTGCAGACCA+AGG 0.588962 5.2:+14911954 MS.gene65317:CDS
TTTGGACAATATCCTTATGG+AGG 0.593205 5.2:+14912968 MS.gene65317:CDS
CTGTAGAAGACTAATGGAGC+TGG 0.594388 5.2:-14909735 None:intergenic
CGAAGGCCATGTGGAGACGA+TGG 0.595336 5.2:-14912668 None:intergenic
CCTCTGATTACATGTGGTGG+TGG 0.595383 5.2:-14910370 None:intergenic
TAATGCTGATGGAATCATAG+AGG 0.596258 5.2:+14912368 MS.gene65317:CDS
CACTCATCCTTACCCTCGAA+GGG 0.596982 5.2:+14909902 MS.gene65317:CDS
GTACTGGACAACGAAAAGAA+TGG 0.597403 5.2:+14909790 MS.gene65317:CDS
AATACAAGGAGACATAGTCT+TGG 0.597621 5.2:-14912760 None:intergenic
CTTGGAACATACATCACACC+TGG 0.601033 5.2:-14910088 None:intergenic
ATATAGAGGATTAGCAGTTG+AGG 0.604087 5.2:+14912643 MS.gene65317:intron
GATGAGTGCTAGACCCTCCA+AGG 0.604496 5.2:-14909887 None:intergenic
TGAATTACAAACTTGGTGGA+AGG 0.608098 5.2:+14912814 MS.gene65317:CDS
ACTTAGTCGAAGATGGATCC+CGG 0.609977 5.2:+14913012 MS.gene65317:CDS
GATCATAGCCAAAAGATCAA+GGG 0.615451 5.2:+14906111 MS.gene65317:CDS
TTTATTGTGTCACGGAAATG+GGG 0.616340 5.2:-14912315 None:intergenic
TGTAGAAGGCGAATCACACA+AGG 0.616729 5.2:-14911237 None:intergenic
CAGCTGGAGATTAATCTAGG+TGG 0.616897 5.2:+14911605 MS.gene65317:CDS
CGTGACACAATAAATATCAA+CGG 0.620852 5.2:+14912324 MS.gene65317:CDS
TTATAGTTAGCAAATCTGCA+TGG 0.622128 5.2:+14911169 MS.gene65317:intron
TACCAACTGATCTTATCAAG+AGG 0.624556 5.2:+14912469 MS.gene65317:CDS
CTTGTATTATCCAACTGATG+AGG 0.626923 5.2:+14912775 MS.gene65317:CDS
ATCAGCAGCATACAAGAACT+GGG 0.628225 5.2:+14912428 MS.gene65317:CDS
ATCCATCTTCGACTAAGTGT+CGG 0.630524 5.2:-14913007 None:intergenic
AAATTGTACTATCAACACCT+TGG 0.634160 5.2:-14911971 None:intergenic
TACCCTCGAAGGGTTAGAAG+TGG 0.635885 5.2:+14909912 MS.gene65317:CDS
CTCCCAAGGTTGGTAGAGTG+GGG 0.636848 5.2:-14909324 None:intergenic
GTTGATCAGTGCTACCAAGA+CGG 0.640155 5.2:+14906302 MS.gene65317:CDS
TGATGGAACTTTGAAACCAG+TGG 0.644367 5.2:+14911875 MS.gene65317:CDS
TAGGTTGAATACTCATGCAG+TGG 0.652665 5.2:+14912224 MS.gene65317:intron
ATCACAAGACGAAGGCCATG+TGG 0.656555 5.2:-14912677 None:intergenic
GAGGATCCATCGTCTCCACA+TGG 0.659512 5.2:+14912662 MS.gene65317:CDS
CCACCACCACATGTAATCAG+AGG 0.659952 5.2:+14910370 MS.gene65317:CDS
GAAGTGCGTGACCTATTTGA+AGG 0.661942 5.2:+14910250 MS.gene65317:CDS
ATCTTCGACTAAGTGTCGGA+CGG 0.684688 5.2:-14913003 None:intergenic
GTGACTTGAAAGACAAACCA+TGG 0.687567 5.2:+14912858 MS.gene65317:CDS
CCAAAAGATCAAGGGCACTG+TGG 0.697540 5.2:+14906119 MS.gene65317:CDS
GATCCTAAAAGTGAGAGACC+AGG 0.704866 5.2:+14910070 MS.gene65317:intron
GTACCTCTGATTACATGTGG+TGG 0.712492 5.2:-14910373 None:intergenic
AGTCCTCGATCACAAGACGA+AGG 0.713341 5.2:-14912685 None:intergenic
GTAACGTCCGTCAAACAAGG+TGG 0.717170 5.2:+14906177 MS.gene65317:CDS
AATGTACCTCTGATTACATG+TGG 0.724970 5.2:-14910376 None:intergenic
GTGCGTGACCTATTTGAAGG+TGG 0.729776 5.2:+14910253 MS.gene65317:CDS
GGACTTGTTCACCATCAGTG+CGG 0.739041 5.2:-14910339 None:intergenic
TAGGCCGGAACGTATCTATG+CGG 0.743680 5.2:+14906280 MS.gene65317:CDS
ACTTGAAAGACAAACCATGG+TGG 0.743773 5.2:+14912861 MS.gene65317:CDS
GTAGAAGGCGAATCACACAA+GGG 0.752151 5.2:-14911236 None:intergenic
CATGTGGTGGTGGAAACTTG+AGG 0.782802 5.2:-14910360 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr5.2 gene 14906090 14913301 14906090 ID=MS.gene65317
chr5.2 mRNA 14906090 14913301 14906090 ID=MS.gene65317.t1;Parent=MS.gene65317
chr5.2 exon 14906090 14906327 14906090 ID=MS.gene65317.t1.exon1;Parent=MS.gene65317.t1
chr5.2 CDS 14906090 14906327 14906090 ID=cds.MS.gene65317.t1;Parent=MS.gene65317.t1
chr5.2 exon 14909275 14909564 14909275 ID=MS.gene65317.t1.exon2;Parent=MS.gene65317.t1
chr5.2 CDS 14909275 14909564 14909275 ID=cds.MS.gene65317.t1;Parent=MS.gene65317.t1
chr5.2 exon 14909714 14909954 14909714 ID=MS.gene65317.t1.exon3;Parent=MS.gene65317.t1
chr5.2 CDS 14909714 14909954 14909714 ID=cds.MS.gene65317.t1;Parent=MS.gene65317.t1
chr5.2 exon 14910071 14910391 14910071 ID=MS.gene65317.t1.exon4;Parent=MS.gene65317.t1
chr5.2 CDS 14910071 14910391 14910071 ID=cds.MS.gene65317.t1;Parent=MS.gene65317.t1
chr5.2 exon 14911175 14911260 14911175 ID=MS.gene65317.t1.exon5;Parent=MS.gene65317.t1
chr5.2 CDS 14911175 14911260 14911175 ID=cds.MS.gene65317.t1;Parent=MS.gene65317.t1
chr5.2 exon 14911536 14911643 14911536 ID=MS.gene65317.t1.exon6;Parent=MS.gene65317.t1
chr5.2 CDS 14911536 14911643 14911536 ID=cds.MS.gene65317.t1;Parent=MS.gene65317.t1
chr5.2 exon 14911756 14912057 14911756 ID=MS.gene65317.t1.exon7;Parent=MS.gene65317.t1
chr5.2 CDS 14911756 14912057 14911756 ID=cds.MS.gene65317.t1;Parent=MS.gene65317.t1
chr5.2 exon 14912227 14912490 14912227 ID=MS.gene65317.t1.exon8;Parent=MS.gene65317.t1
chr5.2 CDS 14912227 14912490 14912227 ID=cds.MS.gene65317.t1;Parent=MS.gene65317.t1
chr5.2 exon 14912649 14913063 14912649 ID=MS.gene65317.t1.exon9;Parent=MS.gene65317.t1
chr5.2 CDS 14912649 14913063 14912649 ID=cds.MS.gene65317.t1;Parent=MS.gene65317.t1
chr5.2 exon 14913182 14913301 14913182 ID=MS.gene65317.t1.exon10;Parent=MS.gene65317.t1
chr5.2 CDS 14913182 14913301 14913182 ID=cds.MS.gene65317.t1;Parent=MS.gene65317.t1
Gene Sequence

>MS.gene65317

ATGTTTGGCATATTTGATAGGGATCATAGCCAAAAGATCAAGGGCACTGTGGTGTTGATGCCCAAAAATGTTTTGGACTTCAACGCTGTAACGTCCGTCAAACAAGGTGGTCTTCTTGATGCTGCCGGGAACATTCTTGATGCTGCCGGCAGCTTGGTCGGCGGGATAATTGACGGTGCTACTGCCTTCTTAGGCCGGAACGTATCTATGCGGTTGATCAGTGCTACCAAGACGGATGGTTTGTCATCTCTACCTAGTTATTTACGTTTATTCAACTCATTCTTTCCATATATATTTCAAAAGAGACAAATATTTTATGATTTTGGAATTTTCACAAAATTATTAGTATGCAAGCCAACTCACCACATTTTTATATTAAATATTAAGTGTGATCAATAATAGATTGATCCAAGTAGTAAGAAATTTGGAACTTTTAAGTAAGTGGTTGGGGAGTCGATCTTTAACTTTTAAGTATGGAGAAATTTCGATTGAGAGAAGAGAACCACTCTTGTGTGCTCAACAAATTTTTTCACGGTGATTAGTTAACGTTAAAAGACAGAGAAAACTTTGTAATTATAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATAGAGACAGGATCCGTTGACACCAGGTGTAAGTCTAATGACTTACACCAAATCTCAACCATTAATTCGAATCAATCAAGTGGCTAATAATTCACAATATATCCAATTAAAAAAAACCCAAAAACTGTGGCACATGTGATCCGTCTCCTTCATCTTCTCTAATTTTATTCGCAGCCTACCAAACTTGTTACCTTGAATAAATATTTTCCAGAACAACATCAGCTCACCGTCTTATAAGTCAATGGTAGAAATTCAATTTGAATTTATCTTATGATGATGCCAAATTAAAAAGAATTATCCCCAATTTTGGAAGAAGAAGAAAATCAAGATTTGAGGGTTATTTGTATCATCGTAACAAGAAATTGCAAGTCTCAGGTGTAATCAAACAAAAGGAATTTATTTCCATTAGTGTTGAGATTCAGAAATTAACACCTTAATCTATTTATAATTTTATCTCAATGATTGATGATTGATGGTTCAACACTAGTAATTGAGATTCAATAAACCACAAAAATTGCAGATCGTAAAAATAAATGAATTTAGGTAGAAGGTCTTTGAAAAAATAGCATGTAAAGAAAAACTATCTAAATGATCACATAAAATAAAGGGAATGGCTGACATCGATCAATGAACGATAACCTGAAAGAGATGAAGGAGACAGATTGTATGTGCCACTGTTTTTGGGGTTTTTTTAATTGGATATATTGTGAGTTATTAGCCACTTGATTGATTGGAATTAATGGTTGAGATTTGGTGTAAGTCATTAGACTTACACCTGGTGTCAATGGATCCTGCCTCATATATATATATATATATATATATATGAGATTTGCTAGAACACACCCACTAGTTTTTATGTGGGAGTGTTTTAGCAAGCTACACCTTAAGGTGTATTTAACTACTTTTTAGTTATAACTTGCTAAAACACACCTTCTAAAAAATAAGTGGGTGTATCCTAGCAAATGCCTATAGGAGTTGCTAACATACACTCACTTATTTTTTAGAAGGTGTGTTTTAGCAACATTCACCTTAAGGTGTATTTAACAACTTTTTAGTTATATCTTTGCTAAAACACACCTTAATAAAAACTAGTGGGTGTATCCTAGCAAACCCCTATATGTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATCAAGTCCATGATGCTACAATATATAGTATACATATAAATAATTTTTTAGAACTTCAATTAATGAGGTCGGATCGTTGAAGAATAACTGAAGATTGAATCTTAACGGATACAATCTTTATTCAAATTTTACGTACCTTTTAATTAAACTTTAAATCATTAAAGTCTCTTTCTTTAGAAACTAGAGGTTTGAAGATCCAAAAAATATAATAATTTTTTAGTAAATTAATGGTGACGCACAATGGTATAAGTTATATAGTAAACAAGAATTTTGTGTTGTTCCCTATTGGAAAGAAAAAAATTTGTGTTGTTAGTAAGGTCTAGCCAACTCAACTAATAATATTGACAGCGTTAGATTAAACACTTTCGTCTGAATTTCAATCTAAAACTCACAATGATATATGTAAGTTTTAGGTCGTGTTTACCATCCATTCTTAAAAAAAATAGGTGTCTTGTGTTTGATATTCACTCTAATAATAATTTGATAGAAACTAGTGGCCGATACATGTGTGACCTACTTGAATTTGTTTATTCAACTAATGAATATTTACACAAGTGGTCCTTGTTTATTCTAAAGTAATCCTTTTTTTAAACGGTCATTCATAGAATGAGTCAAAAAATGATTATTCACACGAACCCCTTTTTTAATGTTTAGGGTTTTGAATATTATATACACTAGTGACTCTTTCTTAATGATTATGGAGGATGAATTGGGTGACTTTACTATTTGATAATTTTGGTGATTTTAGCGTATCTAAATGATAAATGAATATTAAAATAAGAGTTTTTCTATCCAATGTAATATTTGGATAGTTTAGGATAGCTAAAAATTATAGTTTTGTTATTGAAAAAAACAATTATTTATTACTTCATGCGTTTCTAAATATATGTCGTACTTAACAACTTCAATAGACAATTTTGACCATTATTATTTTAATTATCTATCATAAAAAATTATAAAAAAATATATTTTGAAAATATTTATTGAAACAAATCAAACAACATCTTACATGTTAACATTTGTATTATATATTAGTTAAACAATATATTCAAAGTAAGTTATATGAATAGTATAAAACTGCGATATGTATTTAGAAACGGACGGAATACAAAATTATATATTTAATATATATAAAGTGTAAAGTTTTATTGACTTCTTATGCTATGCAAATTTACACATAATTAAATGAGAAAGAATGATTGATTGAAAGAGATAAAGATAAAGTTACCAAAGTTTTAAATGTAGTCTATTTTGATCATGATTATGACTAATATTTGATCACGAATAAATAGCATTTTCCTTTATTTGTTGGAATATGTTATTTTTGTAGCAAATGGCAAGGGATTAGTTGGAAAAGAAGTATTTTTGGAGAAACATGTCCCCACTCTACCAACCTTGGGAGCAAGACAAGATGCATTTACCATTCACTTTGATTGGGATGCTGATTTTGGAATTCCAGGAGCATTTTACATAAGGAACTACATGCAAGCTCACGAGTTCTTTCTTGTTAGTGTCACTTTTGATGACATTCCTAATCGTGAATCCGTTGAATTTGTTTGCAATTCATGGATTTACAATTTCAAAAACTACAAAAAGGATCGCATCTTCTTTACCAATGATGTAAGCTCCAAATCCTTAATTAAAAAAGTTAACTATGTTTACGATTTCTAAAAAATTGACAAAAATTGTTCCATTTTAATTTTTGGCTCTATAATTTTTTATGCATTTTTATCTCCTAAAATATTAAATATTAATTGAATTTGTTATAGACATATCTTCCAAGTCAAACACCAGCTCCATTAGTCTTCTACAGACAAGAAGAATTGCAAAATCTAAGAGGAGATGGTACTGGACAACGAAAAGAATGGGAAAGAGTTTATGATTATGATGTCTACAATGATCTTGGTGATCCAGATGAAGATGTCAAGCTTGCTCGTCCAGTCCTTGGAGGGTCTAGCACTCATCCTTACCCTCGAAGGGTTAGAAGTGGTCGAAAACCAACTAAAAAGGGTAATTTTTTTTATTTTCATATACTTTAAAGACTAAATATGAGAGTCAATAATGTGGTTCTAATGTTGTCTTATTTTTTTTATTTCTTATTCTAATGATATTTTTATTGTTGACAGATCCTAAAAGTGAGAGACCAGGTGTGATGTATGTTCCAAGAGATGAAAACTTTGGACACTTGAAGTCATCTGACTTCCTTACATATGGAATAAAATCATTGTCTCAAGATGTGTTACCTTTGTTTAAATCTGTAATCTTTGATTTGAATTTCACTCCAAATGAGTTTGATAGCTTCGATGAAGTGCGTGACCTATTTGAAGGTGGTATTGAACTTCCTACACACATATTGAGCAAAATCAGTCCCTTACCAGTCCTTAAGGAAATTTTCCGCACTGATGGTGAACAAGTCCTCAAGTTTCCACCACCACATGTAATCAGAGGTACATTATTATTCATCCTTATTCAATCATATGCCTCTATAAATATAGGGTCATGCTAACATATGCCCTAAAAGCAGTGGCGGAGCCAGACAAAAAAATTTGGGGTGGCCGCCATCAAAATAAACTAAAATATATAAATCATATTGCATATAACACATTTAAGTTGTAATAAACCAAAAATCGATCATTTAAAATTTAAACTACATAATATATAAAGTCGATCATTAAATACTTAAAGAGACGATTTACGTTTTCCGAGTGACTTGAAATCATCAATAATTGACTCGAAACTAATACATGCACTAATTTCCCTTTCAATATAAACTGGCATGCTACCGGCTAGAAACTCAGATTCCATCTTGTTTCTCAATTTAACAATATGTAATGAACAAGAATGATTGAAATTGGCAAAGGAGACAAAGTTAAGTGTACTAGACAAGAAAAATGGGTTTATTAATTCCTCAAAAAAAAAAATGGGTTTATTAAAGTATCATTTACAATTCTGCCAAATTAATTATGCAAATTCTAGGGTATAAAAAAAATTATTTTTTTTAAGATGGGGTGGCCAAGGCTCCCCCTTGCCACCCCCTGGTTCCGCCAGTGCCTAAAAGCACATGTTAAAAACAATTGGCATATTTTCCAATGCAATATTTATATCTTTGATACTTTAACATGTGCCCTAACCCTTAGGGTAAATTTTACCATTATACTAGTAGCGATAAAAGAAACATGTGCATTTATTGAATTAGTCCAATTTAATATATATTTCTTATTTTTTTATAGTTAGCAAATCTGCATGGATGACTGACGAAGAATTTGGAAGAGAGATGGTTGCTGGTGTAAACCCTTGTGTGATTCGCCTTCTACAAGTAAGTTTCAAATGTATTTTTTATCATCAAAAGTCATAGATATTATTTACAATTTTTTTTTGGTACATTCAATTTGTTTATTTTATAACCACTAATATATTTTTTTATCTATCATATCTATATCCATGAGGGTAAGAGTTTGATCTTTTAGTCTTTTAGAGATATTGGAATTGGTTGTAAAATGACCAATGCTGCCAATTTAATGAATAATGCTCCACATTCCACAATTTAATATTTTTAACTCATATCATTGTATTAAATTATTTGTTTGGCAGGAGTTCCCACCGAAAAGCACGTTAGATATTGCTGTCTATGGTGATCAAACTAGTATACTAAAAAAAGAGCAGCTGGAGATTAATCTAGGTGGGCTCACAGTAGAAAAGGTAATGATATAAATAGTATAAAATATGTTTCACATAAAAGTCATTATTAATTGGCTTATAAATTATTTAAATTTATGAAAACTAAGTGATTGAAATTATACTTATGGAACAGGCACTTAATGGTCAAAGATTATTCATCTTAGATTATCATGATGCATTCATGCCATTTTTGGAGAAGATAAACAAAAATGCCAAAGCTTATGCCACTAGGACAATCCTTTTCTTAAAAGATGATGGAACTTTGAAACCAGTGGCCATAGAGTTGAGTTTACCACATCCAAACGGTGTTAAGTATGGTGCTGAAAGCAAAATCATCTTGCCTGCAGACCAAGGTGTTGATAGTACAATTTGGCTATTGGCCAAGGCTCATGTAATTGTAAATGACTCATGCTACCATCAACTCATGAGCCACTGGTATGGCCCCTACTAACTAAATCTACCACTCACTCTCTCATTTTTTATTCATTTGTAGTTTTTTCTTAATCTGTGTGAAATGACCTAATATGACGGTTTTTGTAGAACGGAGTGAGTAATAAGTTTAGTATAAGCAATGTATAATACTTTTTTTCAATGTGTATACTAGGTTGAATACTCATGCAGTGGTTGAGCCATTCATCATAGCAACAAACAGACATCTTAGTGTGCTTCACCCTATTAACAAGTTGCTAGATCCCCATTTCCGTGACACAATAAATATCAACGGACTTGCTCGAAATGCTCTAATTAATGCTGATGGAATCATAGAGGAAACATTTTTACCTGGGCCGAATTCTGTTGAGATGTCATCAGCAGCATACAAGAACTGGGTTTTCACTGATCAAGCATTACCAACTGATCTTATCAAGAGGTAATTTGCCACCATCTAATTTGGATGCATATTTCAATTATGCTAGTGTTGTAAATAAATTTTTCTCTACCAATCTAGCGGCCAATTTAAAAAATTGGTCGCTAAATTGATAACAAATTGTTAACTTTTATTGATAAGAAATTGTTTGCTTGATATAGAGGATTAGCAGTTGAGGATCCATCGTCTCCACATGGCCTTCGTCTTGTGATCGAGGACTACCCTTATGCAGTTGACGGACTAGAGATATGGGATGCTATTAAGTCATGGGTCCAAGACTATGTCTCCTTGTATTATCCAACTGATGAGGCAGTACAAAATGACACTGAATTACAAACTTGGTGGAAGGAAGTTGTAGAAAAAGGTCACGGTGACTTGAAAGACAAACCATGGTGGCCTAAGATGCAGAATATTCAAGATTTAATCCAATCATGTTCCATTATCATATGGACTGCTTCTGCTCTTCATGCAGCTGTTAATTTTGGACAATATCCTTATGGAGGTTATATTCTGAACCGTCCGACACTTAGTCGAAGATGGATCCCGGAGAAAGGAACTCCAAATTATGATGAGATGGTGAAAAACCCTCAAAAAGCATATTTGAGAACAATTACACCAAAATATCAAACCCTTATTGATCTTTCTGTGATTGAGATATTGTCAAGACATGCTTCTGATGAAATCTACCTTGGAGAGAGGGATGTTAAGTTTTGGACATCTGATTCAAGAGCATTACAAGCCTTTCAAAAGTTTGGAAGTAAGCTGGCCAAAATTGAAGGAAAAATCACAGAAAGAAACAATGATTCCGATCTGA
Protein sequence

>MS.gene65317.t1

MFGIFDRDHSQKIKGTVVLMPKNVLDFNAVTSVKQGGLLDAAGNILDAAGSLVGGIIDGATAFLGRNVSMRLISATKTDANGKGLVGKEVFLEKHVPTLPTLGARQDAFTIHFDWDADFGIPGAFYIRNYMQAHEFFLVSVTFDDIPNRESVEFVCNSWIYNFKNYKKDRIFFTNDTYLPSQTPAPLVFYRQEELQNLRGDGTGQRKEWERVYDYDVYNDLGDPDEDVKLARPVLGGSSTHPYPRRVRSGRKPTKKDPKSERPGVMYVPRDENFGHLKSSDFLTYGIKSLSQDVLPLFKSVIFDLNFTPNEFDSFDEVRDLFEGGIELPTHILSKISPLPVLKEIFRTDGEQVLKFPPPHVIRVSKSAWMTDEEFGREMVAGVNPCVIRLLQEFPPKSTLDIAVYGDQTSILKKEQLEINLGGLTVEKALNGQRLFILDYHDAFMPFLEKINKNAKAYATRTILFLKDDGTLKPVAIELSLPHPNGVKYGAESKIILPADQGVDSTIWLLAKAHVIVNDSCYHQLMSHWLNTHAVVEPFIIATNRHLSVLHPINKLLDPHFRDTININGLARNALINADGIIEETFLPGPNSVEMSSAAYKNWVFTDQALPTDLIKRGLAVEDPSSPHGLRLVIEDYPYAVDGLEIWDAIKSWVQDYVSLYYPTDEAVQNDTELQTWWKEVVEKGHGDLKDKPWWPKMQNIQDLIQSCSIIIWTASALHAAVNFGQYPYGGYILNRPTLSRRWIPEKGTPNYDEMRGMLSFGHLIQEHYKPFKSLEVSWPKLKEKSQKETMIPI