Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67382.t1 | XP_003606593.1 | 95.3 | 464 | 22 | 0 | 1 | 464 | 1 | 464 | 2.30E-261 | 911 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67382.t1 | Q9LQP8 | 69.3 | 475 | 132 | 4 | 1 | 461 | 1 | 475 | 1.9e-195 | 683.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67382.t1 | G7JSP5 | 95.3 | 464 | 22 | 0 | 1 | 464 | 1 | 464 | 1.6e-261 | 911.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049346 | MS.gene67382 | 0.824996 | 5.96E-54 | -1.69E-46 |
| MS.gene049491 | MS.gene67382 | 0.821448 | 4.02E-53 | -1.69E-46 |
| MS.gene049681 | MS.gene67382 | 0.814234 | 1.71E-51 | -1.69E-46 |
| MS.gene049758 | MS.gene67382 | 0.852975 | 3.24E-61 | -1.69E-46 |
| MS.gene049910 | MS.gene67382 | 0.808912 | 2.45E-50 | -1.69E-46 |
| MS.gene050128 | MS.gene67382 | 0.855519 | 5.97E-62 | -1.69E-46 |
| MS.gene050301 | MS.gene67382 | 0.827879 | 1.23E-54 | -1.69E-46 |
| MS.gene050397 | MS.gene67382 | 0.840494 | 8.41E-58 | -1.69E-46 |
| MS.gene050464 | MS.gene67382 | 0.812588 | 3.93E-51 | -1.69E-46 |
| MS.gene050529 | MS.gene67382 | 0.806407 | 8.35E-50 | -1.69E-46 |
| MS.gene050670 | MS.gene67382 | 0.80998 | 1.45E-50 | -1.69E-46 |
| MS.gene050762 | MS.gene67382 | 0.802684 | 4.99E-49 | -1.69E-46 |
| MS.gene050763 | MS.gene67382 | 0.806827 | 6.81E-50 | -1.69E-46 |
| MS.gene050971 | MS.gene67382 | 0.842725 | 2.17E-58 | -1.69E-46 |
| MS.gene05101 | MS.gene67382 | 0.80072 | 1.26E-48 | -1.69E-46 |
| MS.gene051471 | MS.gene67382 | 0.810417 | 1.16E-50 | -1.69E-46 |
| MS.gene051663 | MS.gene67382 | 0.805423 | 1.34E-49 | -1.69E-46 |
| MS.gene051860 | MS.gene67382 | 0.836443 | 9.33E-57 | -1.69E-46 |
| MS.gene052077 | MS.gene67382 | 0.816422 | 5.58E-52 | -1.69E-46 |
| MS.gene052078 | MS.gene67382 | 0.814022 | 1.90E-51 | -1.69E-46 |
| MS.gene052178 | MS.gene67382 | 0.848312 | 6.64E-60 | -1.69E-46 |
| MS.gene052188 | MS.gene67382 | 0.838279 | 3.16E-57 | -1.69E-46 |
| MS.gene052253 | MS.gene67382 | 0.82013 | 8.07E-53 | -1.69E-46 |
| MS.gene052310 | MS.gene67382 | 0.833012 | 6.81E-56 | -1.69E-46 |
| MS.gene052326 | MS.gene67382 | 0.80026 | 1.57E-48 | -1.69E-46 |
| MS.gene052340 | MS.gene67382 | 0.866333 | 3.08E-65 | -1.69E-46 |
| MS.gene052485 | MS.gene67382 | 0.830448 | 2.92E-55 | -1.69E-46 |
| MS.gene053073 | MS.gene67382 | 0.821132 | 4.75E-53 | -1.69E-46 |
| MS.gene05316 | MS.gene67382 | 0.835992 | 1.21E-56 | -1.69E-46 |
| MS.gene053557 | MS.gene67382 | 0.829837 | 4.12E-55 | -1.69E-46 |
| MS.gene053813 | MS.gene67382 | 0.843811 | 1.11E-58 | -1.69E-46 |
| MS.gene053836 | MS.gene67382 | 0.865884 | 4.27E-65 | -1.69E-46 |
| MS.gene053928 | MS.gene67382 | 0.810621 | 1.05E-50 | -1.69E-46 |
| MS.gene054010 | MS.gene67382 | 0.812755 | 3.61E-51 | -1.69E-46 |
| MS.gene054011 | MS.gene67382 | 0.832962 | 7.01E-56 | -1.69E-46 |
| MS.gene054012 | MS.gene67382 | 0.828324 | 9.58E-55 | -1.69E-46 |
| MS.gene054013 | MS.gene67382 | 0.82969 | 4.47E-55 | -1.69E-46 |
| MS.gene054123 | MS.gene67382 | 0.800561 | 1.36E-48 | -1.69E-46 |
| MS.gene054214 | MS.gene67382 | 0.80151 | 8.70E-49 | -1.69E-46 |
| MS.gene054349 | MS.gene67382 | 0.82098 | 5.15E-53 | -1.69E-46 |
| MS.gene054473 | MS.gene67382 | 0.804215 | 2.40E-49 | -1.69E-46 |
| MS.gene054542 | MS.gene67382 | 0.801651 | 8.14E-49 | -1.69E-46 |
| MS.gene055116 | MS.gene67382 | 0.834688 | 2.59E-56 | -1.69E-46 |
| MS.gene055277 | MS.gene67382 | 0.856941 | 2.29E-62 | -1.69E-46 |
| MS.gene055422 | MS.gene67382 | 0.841902 | 3.58E-58 | -1.69E-46 |
| MS.gene055428 | MS.gene67382 | 0.827156 | 1.83E-54 | -1.69E-46 |
| MS.gene055604 | MS.gene67382 | 0.811891 | 5.58E-51 | -1.69E-46 |
| MS.gene056191 | MS.gene67382 | 0.874769 | 5.21E-68 | -1.69E-46 |
| MS.gene056407 | MS.gene67382 | 0.821522 | 3.86E-53 | -1.69E-46 |
| MS.gene056410 | MS.gene67382 | 0.807023 | 6.19E-50 | -1.69E-46 |
| MS.gene056612 | MS.gene67382 | 0.808519 | 2.98E-50 | -1.69E-46 |
| MS.gene056899 | MS.gene67382 | -0.800032 | 1.74E-48 | -1.69E-46 |
| MS.gene05702 | MS.gene67382 | 0.844119 | 9.21E-59 | -1.69E-46 |
| MS.gene05733 | MS.gene67382 | 0.802798 | 4.73E-49 | -1.69E-46 |
| MS.gene057448 | MS.gene67382 | 0.813251 | 2.81E-51 | -1.69E-46 |
| MS.gene057449 | MS.gene67382 | 0.811353 | 7.31E-51 | -1.69E-46 |
| MS.gene057450 | MS.gene67382 | 0.819861 | 9.30E-53 | -1.69E-46 |
| MS.gene057749 | MS.gene67382 | 0.814782 | 1.29E-51 | -1.69E-46 |
| MS.gene057779 | MS.gene67382 | 0.83454 | 2.83E-56 | -1.69E-46 |
| MS.gene057782 | MS.gene67382 | 0.818294 | 2.11E-52 | -1.69E-46 |
| MS.gene057796 | MS.gene67382 | 0.827095 | 1.89E-54 | -1.69E-46 |
| MS.gene057799 | MS.gene67382 | 0.812658 | 3.79E-51 | -1.69E-46 |
| MS.gene057823 | MS.gene67382 | 0.811659 | 6.27E-51 | -1.69E-46 |
| MS.gene058002 | MS.gene67382 | 0.82578 | 3.89E-54 | -1.69E-46 |
| MS.gene05811 | MS.gene67382 | 0.839513 | 1.51E-57 | -1.69E-46 |
| MS.gene058660 | MS.gene67382 | 0.829263 | 5.68E-55 | -1.69E-46 |
| MS.gene058928 | MS.gene67382 | 0.840197 | 1.01E-57 | -1.69E-46 |
| MS.gene058995 | MS.gene67382 | 0.821048 | 4.97E-53 | -1.69E-46 |
| MS.gene05907 | MS.gene67382 | 0.870905 | 1.02E-66 | -1.69E-46 |
| MS.gene059082 | MS.gene67382 | 0.806351 | 8.58E-50 | -1.69E-46 |
| MS.gene059285 | MS.gene67382 | 0.800951 | 1.13E-48 | -1.69E-46 |
| MS.gene059294 | MS.gene67382 | 0.871888 | 4.85E-67 | -1.69E-46 |
| MS.gene059420 | MS.gene67382 | 0.812412 | 4.30E-51 | -1.69E-46 |
| MS.gene059462 | MS.gene67382 | 0.802251 | 6.13E-49 | -1.69E-46 |
| MS.gene059518 | MS.gene67382 | 0.819566 | 1.09E-52 | -1.69E-46 |
| MS.gene059597 | MS.gene67382 | 0.834815 | 2.41E-56 | -1.69E-46 |
| MS.gene059676 | MS.gene67382 | 0.809974 | 1.45E-50 | -1.69E-46 |
| MS.gene059688 | MS.gene67382 | 0.838881 | 2.21E-57 | -1.69E-46 |
| MS.gene059692 | MS.gene67382 | 0.834774 | 2.47E-56 | -1.69E-46 |
| MS.gene059695 | MS.gene67382 | 0.830673 | 2.57E-55 | -1.69E-46 |
| MS.gene059807 | MS.gene67382 | 0.839847 | 1.24E-57 | -1.69E-46 |
| MS.gene05984 | MS.gene67382 | 0.85396 | 1.69E-61 | -1.69E-46 |
| MS.gene059894 | MS.gene67382 | 0.844751 | 6.23E-59 | -1.69E-46 |
| MS.gene059974 | MS.gene67382 | 0.825064 | 5.75E-54 | -1.69E-46 |
| MS.gene060005 | MS.gene67382 | 0.844001 | 9.90E-59 | -1.69E-46 |
| MS.gene06037 | MS.gene67382 | 0.850855 | 1.30E-60 | -1.69E-46 |
| MS.gene060403 | MS.gene67382 | 0.803474 | 3.43E-49 | -1.69E-46 |
| MS.gene060495 | MS.gene67382 | 0.804525 | 2.07E-49 | -1.69E-46 |
| MS.gene060986 | MS.gene67382 | 0.813954 | 1.97E-51 | -1.69E-46 |
| MS.gene061049 | MS.gene67382 | 0.875247 | 3.58E-68 | -1.69E-46 |
| MS.gene061051 | MS.gene67382 | -0.823036 | 1.72E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67382.t1 | MTR_4g062270 | 95.259 | 464 | 22 | 0 | 1 | 464 | 1 | 464 | 0.0 | 925 |
| MS.gene67382.t1 | MTR_4g062170 | 89.407 | 472 | 42 | 1 | 1 | 464 | 1 | 472 | 0.0 | 871 |
| MS.gene67382.t1 | MTR_4g062190 | 83.510 | 473 | 69 | 1 | 1 | 464 | 1 | 473 | 0.0 | 818 |
| MS.gene67382.t1 | MTR_2g096210 | 69.427 | 471 | 131 | 3 | 1 | 459 | 1 | 470 | 0.0 | 707 |
| MS.gene67382.t1 | MTR_1g115560 | 52.991 | 468 | 202 | 2 | 1 | 450 | 1 | 468 | 0.0 | 536 |
| MS.gene67382.t1 | MTR_7g118170 | 42.791 | 430 | 230 | 5 | 12 | 433 | 104 | 525 | 2.47e-122 | 367 |
| MS.gene67382.t1 | MTR_3g105550 | 38.584 | 438 | 257 | 3 | 6 | 433 | 98 | 533 | 8.52e-112 | 340 |
| MS.gene67382.t1 | MTR_1g076430 | 40.995 | 422 | 240 | 4 | 15 | 433 | 88 | 503 | 1.04e-110 | 337 |
| MS.gene67382.t1 | MTR_4g070270 | 40.455 | 440 | 245 | 8 | 3 | 433 | 68 | 499 | 3.19e-110 | 335 |
| MS.gene67382.t1 | MTR_2g054940 | 39.815 | 432 | 252 | 3 | 5 | 433 | 76 | 502 | 2.82e-109 | 333 |
| MS.gene67382.t1 | MTR_1g041085 | 40.758 | 422 | 242 | 3 | 15 | 433 | 93 | 509 | 2.42e-108 | 331 |
| MS.gene67382.t1 | MTR_5g056510 | 38.889 | 432 | 251 | 6 | 7 | 433 | 64 | 487 | 2.77e-108 | 330 |
| MS.gene67382.t1 | MTR_1g110930 | 37.731 | 432 | 256 | 6 | 7 | 433 | 64 | 487 | 1.95e-107 | 328 |
| MS.gene67382.t1 | MTR_4g113280 | 38.889 | 432 | 245 | 4 | 5 | 433 | 65 | 480 | 1.30e-103 | 318 |
| MS.gene67382.t1 | MTR_7g118160 | 42.015 | 407 | 184 | 10 | 32 | 433 | 57 | 416 | 1.37e-103 | 315 |
| MS.gene67382.t1 | MTR_4g054415 | 37.709 | 419 | 252 | 5 | 19 | 433 | 9 | 422 | 3.08e-100 | 307 |
| MS.gene67382.t1 | MTR_4g054630 | 37.709 | 419 | 252 | 5 | 19 | 433 | 50 | 463 | 3.92e-100 | 308 |
| MS.gene67382.t1 | MTR_2g007020 | 40.146 | 411 | 235 | 6 | 27 | 433 | 45 | 448 | 1.23e-98 | 304 |
| MS.gene67382.t1 | MTR_7g081695 | 38.426 | 432 | 246 | 4 | 5 | 433 | 76 | 490 | 4.22e-98 | 311 |
| MS.gene67382.t1 | MTR_6g074880 | 37.947 | 419 | 251 | 5 | 19 | 433 | 52 | 465 | 3.05e-97 | 300 |
| MS.gene67382.t1 | MTR_6g032965 | 39.141 | 396 | 230 | 6 | 55 | 444 | 2 | 392 | 3.37e-93 | 288 |
| MS.gene67382.t1 | MTR_2g013740 | 37.500 | 392 | 235 | 3 | 29 | 419 | 110 | 492 | 3.20e-92 | 289 |
| MS.gene67382.t1 | MTR_2g436480 | 36.473 | 414 | 249 | 7 | 26 | 433 | 43 | 448 | 1.20e-90 | 283 |
| MS.gene67382.t1 | MTR_1g102290 | 36.430 | 409 | 245 | 8 | 32 | 433 | 83 | 483 | 2.56e-80 | 257 |
| MS.gene67382.t1 | MTR_3g072940 | 33.744 | 406 | 259 | 4 | 32 | 433 | 83 | 482 | 1.75e-77 | 250 |
| MS.gene67382.t1 | MTR_3g072980 | 33.744 | 406 | 259 | 4 | 32 | 433 | 83 | 482 | 1.85e-77 | 250 |
| MS.gene67382.t1 | MTR_6g051440 | 33.990 | 406 | 258 | 4 | 32 | 433 | 82 | 481 | 1.29e-71 | 234 |
| MS.gene67382.t1 | MTR_4g054260 | 39.044 | 251 | 128 | 4 | 187 | 433 | 93 | 322 | 4.14e-51 | 176 |
| MS.gene67382.t1 | MTR_4g053905 | 42.405 | 158 | 84 | 3 | 279 | 433 | 3 | 156 | 5.92e-37 | 133 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67382.t1 | AT1G07720 | 70.563 | 462 | 122 | 4 | 14 | 461 | 57 | 518 | 0.0 | 684 |
| MS.gene67382.t1 | AT1G07720 | 70.563 | 462 | 122 | 4 | 14 | 461 | 14 | 475 | 0.0 | 682 |
| MS.gene67382.t1 | AT2G28630 | 68.860 | 456 | 128 | 4 | 19 | 461 | 19 | 473 | 0.0 | 674 |
| MS.gene67382.t1 | AT2G28630 | 68.860 | 456 | 128 | 4 | 19 | 461 | 19 | 473 | 0.0 | 674 |
| MS.gene67382.t1 | AT5G04530 | 53.881 | 438 | 198 | 3 | 17 | 450 | 18 | 455 | 0.0 | 521 |
| MS.gene67382.t1 | AT1G01120 | 40.793 | 429 | 240 | 5 | 13 | 435 | 105 | 525 | 1.13e-118 | 358 |
| MS.gene67382.t1 | AT2G46720 | 38.952 | 439 | 254 | 4 | 2 | 433 | 31 | 462 | 5.66e-113 | 341 |
| MS.gene67382.t1 | AT1G25450 | 39.723 | 433 | 248 | 6 | 6 | 433 | 59 | 483 | 4.57e-111 | 337 |
| MS.gene67382.t1 | AT2G16280 | 40.625 | 416 | 240 | 3 | 22 | 435 | 99 | 509 | 1.75e-110 | 336 |
| MS.gene67382.t1 | AT4G34510 | 40.670 | 418 | 238 | 4 | 22 | 435 | 73 | 484 | 8.80e-110 | 333 |
| MS.gene67382.t1 | AT1G19440 | 39.773 | 440 | 248 | 7 | 3 | 433 | 80 | 511 | 2.43e-109 | 333 |
| MS.gene67382.t1 | AT2G15090 | 39.770 | 435 | 239 | 7 | 13 | 433 | 51 | 476 | 3.87e-109 | 332 |
| MS.gene67382.t1 | AT1G68530 | 38.799 | 433 | 252 | 6 | 6 | 433 | 64 | 488 | 5.04e-109 | 332 |
| MS.gene67382.t1 | AT1G04220 | 39.597 | 447 | 246 | 8 | 1 | 433 | 76 | 512 | 5.66e-109 | 333 |
| MS.gene67382.t1 | AT2G26640 | 39.352 | 432 | 254 | 3 | 5 | 433 | 74 | 500 | 7.93e-109 | 332 |
| MS.gene67382.t1 | AT5G43760 | 41.509 | 424 | 226 | 7 | 23 | 433 | 103 | 517 | 2.63e-108 | 331 |
| MS.gene67382.t1 | AT3G10280 | 37.813 | 439 | 252 | 5 | 2 | 433 | 31 | 455 | 3.28e-106 | 323 |
| MS.gene67382.t1 | AT4G34250 | 40.426 | 423 | 233 | 7 | 22 | 433 | 70 | 484 | 8.14e-106 | 323 |
| MS.gene67382.t1 | AT2G26250 | 35.927 | 437 | 254 | 3 | 22 | 433 | 112 | 547 | 1.61e-102 | 317 |
| MS.gene67382.t1 | AT4G34520 | 38.053 | 452 | 256 | 8 | 1 | 435 | 45 | 489 | 2.47e-102 | 315 |
| MS.gene67382.t1 | AT5G49070 | 36.721 | 433 | 265 | 6 | 4 | 433 | 26 | 452 | 2.37e-93 | 290 |
| MS.gene67382.t1 | AT1G71160 | 37.192 | 406 | 243 | 5 | 32 | 433 | 48 | 445 | 1.85e-92 | 288 |
| MS.gene67382.t1 | AT3G52160 | 32.840 | 405 | 236 | 3 | 16 | 420 | 73 | 441 | 1.82e-78 | 251 |
| MS.gene67382.t1 | AT3G52160 | 35.530 | 349 | 189 | 3 | 72 | 420 | 16 | 328 | 7.80e-75 | 238 |
| MS.gene67382.t1 | AT1G68530 | 34.932 | 292 | 183 | 4 | 6 | 294 | 64 | 351 | 1.43e-58 | 197 |
| MS.gene67382.t1 | AT5G49070 | 29.655 | 290 | 201 | 2 | 4 | 293 | 26 | 312 | 2.07e-37 | 139 |
Find 91 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCTTTGTTGGCAATATTT+TGG | 0.090289 | 4.1:+37592198 | None:intergenic |
| AAGTATACTTCTGTTCTTTA+AGG | 0.095484 | 4.1:+37592384 | None:intergenic |
| AATCAGAAGAACAGAAAATT+TGG | 0.156705 | 4.1:-37592911 | MS.gene67382:CDS |
| TGTACCTATGTTTCCTTATC+TGG | 0.174264 | 4.1:-37593038 | MS.gene67382:CDS |
| CGGCCTTCAAAGACGTTTCT+TGG | 0.212496 | 4.1:+37592820 | None:intergenic |
| TCAAATGCAATAGTTGCTTA+TGG | 0.231406 | 4.1:-37591859 | MS.gene67382:CDS |
| GAGAAACAAGTTGGCACTTT+TGG | 0.238283 | 4.1:-37592521 | MS.gene67382:CDS |
| TCATTGCCAGTATACCAATT+TGG | 0.269157 | 4.1:+37592478 | None:intergenic |
| AACCTAAGTATTTCCTTTGT+TGG | 0.270627 | 4.1:+37592187 | None:intergenic |
| TAATTATCCACCAAAGTCTT+TGG | 0.310010 | 4.1:-37591786 | MS.gene67382:CDS |
| GGCCTTCAAAGACGTTTCTT+GGG | 0.322434 | 4.1:+37592821 | None:intergenic |
| CGATGTAGTGTCATTCTTGC+AGG | 0.330569 | 4.1:+37591992 | None:intergenic |
| TCAGGAAGTTCATAATTGTT+AGG | 0.335455 | 4.1:+37591716 | None:intergenic |
| GTCTTTGGTCAATCCTTTCA+TGG | 0.341262 | 4.1:-37591771 | MS.gene67382:CDS |
| CAGAGTCTCTCTGTCCAAAT+TGG | 0.352442 | 4.1:-37592492 | MS.gene67382:CDS |
| AGTAGCTTGTGGTATGTGTT+AGG | 0.359473 | 4.1:-37591950 | MS.gene67382:CDS |
| AGATCCCGTCGTTCAGTGTT+GGG | 0.365852 | 4.1:+37592788 | None:intergenic |
| CAAATGCAATAGTTGCTTAT+GGG | 0.367150 | 4.1:-37591858 | MS.gene67382:CDS |
| AAAGCAGTGATTGATGGAAT+TGG | 0.371201 | 4.1:-37592049 | MS.gene67382:CDS |
| CAGCCTCACTTAAGAAAATC+AGG | 0.372574 | 4.1:+37591698 | None:intergenic |
| GACGACAAGAACATCAATTT+CGG | 0.379107 | 4.1:+37592699 | None:intergenic |
| CTCTGTCCAAATTGGTATAC+TGG | 0.380545 | 4.1:-37592484 | MS.gene67382:CDS |
| TCACCATTGAATTTCAAAAC+TGG | 0.383289 | 4.1:-37592103 | MS.gene67382:CDS |
| AATCCTTTCATGGAGAAGTA+TGG | 0.384841 | 4.1:-37591761 | MS.gene67382:CDS |
| AGAATGACACTACATCGTTT+TGG | 0.403512 | 4.1:-37591986 | MS.gene67382:CDS |
| GGTAGGCTTGGTGTTCACTT+AGG | 0.408733 | 4.1:-37592280 | MS.gene67382:CDS |
| GGAAACTATACGATCAAAAG+AGG | 0.421293 | 4.1:-37593017 | MS.gene67382:CDS |
| GGAGGAAAAGCAGTGATTGA+TGG | 0.421764 | 4.1:-37592055 | MS.gene67382:CDS |
| GTAGGCTTGGTGTTCACTTA+GGG | 0.439135 | 4.1:-37592279 | MS.gene67382:CDS |
| CACAACTTACAATGGCTTTG+AGG | 0.439424 | 4.1:+37592866 | None:intergenic |
| ATCAGAAGAACAGAAAATTT+GGG | 0.439708 | 4.1:-37592910 | MS.gene67382:CDS |
| CACTCAAAAGGAAGATGAGC+AGG | 0.441650 | 4.1:-37592302 | MS.gene67382:CDS |
| TGTTGAAGATGCAAATGTTT+GGG | 0.442098 | 4.1:-37591822 | MS.gene67382:CDS |
| CCTAGCATTCTATCTTGTGT+TGG | 0.442413 | 4.1:+37592955 | None:intergenic |
| GCCCCAAGAAACGTCTTTGA+AGG | 0.450479 | 4.1:-37592823 | MS.gene67382:CDS |
| GATCGTATAGTTTCCAGATA+AGG | 0.450987 | 4.1:+37593025 | None:intergenic |
| CAAATCACAGAGAAACAAGT+TGG | 0.460220 | 4.1:-37592530 | MS.gene67382:CDS |
| GTAGCTTGTGGTATGTGTTA+GGG | 0.465874 | 4.1:-37591949 | MS.gene67382:CDS |
| TGCCAACAAAGGAAATACTT+AGG | 0.467344 | 4.1:-37592189 | MS.gene67382:CDS |
| TTACACTTGCTGCACTCAAA+AGG | 0.470098 | 4.1:-37592314 | MS.gene67382:CDS |
| ATGTTGAAGATGCAAATGTT+TGG | 0.488174 | 4.1:-37591823 | MS.gene67382:CDS |
| CTTGTGTTGGCTTGTAACAT+TGG | 0.492229 | 4.1:+37592968 | None:intergenic |
| GAGATCCCGTCGTTCAGTGT+TGG | 0.492490 | 4.1:+37592787 | None:intergenic |
| GCCATTGTAAGTTGTGGCAT+TGG | 0.492812 | 4.1:-37592859 | MS.gene67382:CDS |
| GAAGATGAGCAGGGTAGGCT+TGG | 0.501327 | 4.1:-37592292 | MS.gene67382:CDS |
| GTGTACAATCTCACCGCAAT+GGG | 0.504188 | 4.1:-37592598 | MS.gene67382:CDS |
| TTTCATGGAGAAGTATGGTT+GGG | 0.519323 | 4.1:-37591756 | MS.gene67382:CDS |
| AAAGGATTGACCAAAGACTT+TGG | 0.522713 | 4.1:+37591776 | None:intergenic |
| TTTATGATAAGCTTTGGTGC+TGG | 0.524017 | 4.1:-37591884 | MS.gene67382:CDS |
| GCAAGTGTAAGAGTCATCTC+TGG | 0.525576 | 4.1:+37592327 | None:intergenic |
| TCAGTGTTGGGGATCCTTCG+CGG | 0.526616 | 4.1:+37592800 | None:intergenic |
| AAGAAACTTAAGAAAACTAG+TGG | 0.529244 | 4.1:-37592145 | MS.gene67382:CDS |
| AGGAAACATAGGTACAATGC+TGG | 0.529366 | 4.1:+37593045 | None:intergenic |
| GTGGTATGTGTTAGGGTACA+TGG | 0.531641 | 4.1:-37591942 | MS.gene67382:CDS |
| AATCTAATATGTAACACTCT+TGG | 0.533209 | 4.1:+37592992 | None:intergenic |
| AACGTCTTTGAAGGCCGCGA+AGG | 0.538270 | 4.1:-37592814 | MS.gene67382:CDS |
| AGAAGGAAGAACGGAGAGCA+TGG | 0.542193 | 4.1:+37592669 | None:intergenic |
| CAACCATACTTCTCCATGAA+AGG | 0.543653 | 4.1:+37591758 | None:intergenic |
| CTCAAAGCCATTGTAAGTTG+TGG | 0.544372 | 4.1:-37592865 | MS.gene67382:CDS |
| GATCCCCAACACTGAACGAC+GGG | 0.545206 | 4.1:-37592792 | MS.gene67382:CDS |
| AAGAACATCAATTTCGGAAG+GGG | 0.550157 | 4.1:+37592705 | None:intergenic |
| CCAACACAAGATAGAATGCT+AGG | 0.550403 | 4.1:-37592955 | MS.gene67382:CDS |
| GCCTTCAAAGACGTTTCTTG+GGG | 0.551870 | 4.1:+37592822 | None:intergenic |
| ATGATTCGCGACGATAAAGA+AGG | 0.561187 | 4.1:+37592652 | None:intergenic |
| ACCCAAAATATTGCCAACAA+AGG | 0.563268 | 4.1:-37592200 | MS.gene67382:CDS |
| GCATTGCAAAACTTCTATCA+AGG | 0.565531 | 4.1:-37592738 | MS.gene67382:CDS |
| GTTTCCAGATAAGGAAACAT+AGG | 0.568958 | 4.1:+37593034 | None:intergenic |
| CTTTCATGGAGAAGTATGGT+TGG | 0.572414 | 4.1:-37591757 | MS.gene67382:CDS |
| GCCAATGCCACAACTTACAA+TGG | 0.575890 | 4.1:+37592858 | None:intergenic |
| GATCCCGTCGTTCAGTGTTG+GGG | 0.581474 | 4.1:+37592789 | None:intergenic |
| AGTGTACAATCTCACCGCAA+TGG | 0.584548 | 4.1:-37592599 | MS.gene67382:CDS |
| AAAAGGAAGATGAGCAGGGT+AGG | 0.586228 | 4.1:-37592297 | MS.gene67382:CDS |
| TGGTTGGGTCAATGAAGTTG+AGG | 0.602405 | 4.1:-37591741 | MS.gene67382:CDS |
| TCCAGAACGGAAAAGACAGT+TGG | 0.602463 | 4.1:+37592438 | None:intergenic |
| CAAGAACATCAATTTCGGAA+GGG | 0.610099 | 4.1:+37592704 | None:intergenic |
| ACAAGAACATCAATTTCGGA+AGG | 0.613595 | 4.1:+37592703 | None:intergenic |
| GGATTGACCAAAGACTTTGG+TGG | 0.615272 | 4.1:+37591779 | None:intergenic |
| ACACTTCTGCTAGTAGCTTG+TGG | 0.618863 | 4.1:-37591961 | MS.gene67382:CDS |
| CGACGATAAAGAAGGAAGAA+CGG | 0.620250 | 4.1:+37592660 | None:intergenic |
| TTTCCGTTCTGGAGGATGCG+CGG | 0.621547 | 4.1:-37592428 | MS.gene67382:CDS |
| GAAGTACATAAATTAAAGAG+AGG | 0.630019 | 4.1:+37593067 | None:intergenic |
| AAACTTAAGAAAACTAGTGG+AGG | 0.638676 | 4.1:-37592142 | MS.gene67382:CDS |
| GAAACTATACGATCAAAAGA+GGG | 0.639622 | 4.1:-37593016 | MS.gene67382:CDS |
| ACTAGCACTGCAACCCATTG+CGG | 0.642619 | 4.1:+37592585 | None:intergenic |
| CAACACAAGATAGAATGCTA+GGG | 0.647129 | 4.1:-37592954 | MS.gene67382:CDS |
| GAACCGCGCATCCTCCAGAA+CGG | 0.647525 | 4.1:+37592425 | None:intergenic |
| AAGAGTCATCTCTGGCGCCG+TGG | 0.648958 | 4.1:+37592335 | None:intergenic |
| ACTCAAAAGGAAGATGAGCA+GGG | 0.674433 | 4.1:-37592301 | MS.gene67382:CDS |
| GGATCCCCAACACTGAACGA+CGG | 0.688356 | 4.1:-37592793 | MS.gene67382:CDS |
| ATGCTAGGGACAGAGTTCTG+TGG | 0.705770 | 4.1:-37592940 | MS.gene67382:CDS |
| TGTTTAGTACGAACGCACCA+CGG | 0.710347 | 4.1:-37592352 | MS.gene67382:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGCTTTTGTTGATAATTTA+AGG | - | chr4.1:37592547-37592566 | MS.gene67382:CDS | 20.0% |
| !!! | AGCTTTTGTTGATAATTTAA+GGG | - | chr4.1:37592548-37592567 | MS.gene67382:CDS | 20.0% |
| ! | AAGAAACTTAAGAAAACTAG+TGG | - | chr4.1:37592633-37592652 | MS.gene67382:CDS | 25.0% |
| ! | AAGTATACTTCTGTTCTTTA+AGG | + | chr4.1:37592397-37592416 | None:intergenic | 25.0% |
| ! | AATCAGAAGAACAGAAAATT+TGG | - | chr4.1:37591867-37591886 | MS.gene67382:CDS | 25.0% |
| ! | AATCTAATATGTAACACTCT+TGG | + | chr4.1:37591789-37591808 | None:intergenic | 25.0% |
| ! | ATCAGAAGAACAGAAAATTT+GGG | - | chr4.1:37591868-37591887 | MS.gene67382:CDS | 25.0% |
| ! | GAAGTACATAAATTAAAGAG+AGG | + | chr4.1:37591714-37591733 | None:intergenic | 25.0% |
| !! | AGAGTTTTTATGATAAGCTT+TGG | - | chr4.1:37592888-37592907 | MS.gene67382:CDS | 25.0% |
| AAACTTAAGAAAACTAGTGG+AGG | - | chr4.1:37592636-37592655 | MS.gene67382:CDS | 30.0% | |
| AACCTAAGTATTTCCTTTGT+TGG | + | chr4.1:37592594-37592613 | None:intergenic | 30.0% | |
| ATGTTGAAGATGCAAATGTT+TGG | - | chr4.1:37592955-37592974 | MS.gene67382:CDS | 30.0% | |
| CAAATGCAATAGTTGCTTAT+GGG | - | chr4.1:37592920-37592939 | MS.gene67382:CDS | 30.0% | |
| GAAACTATACGATCAAAAGA+GGG | - | chr4.1:37591762-37591781 | MS.gene67382:CDS | 30.0% | |
| GCAAAAAAGAGGCTAAAAAA+AGG | - | chr4.1:37592861-37592880 | MS.gene67382:CDS | 30.0% | |
| TAATTATCCACCAAAGTCTT+TGG | - | chr4.1:37592992-37593011 | MS.gene67382:CDS | 30.0% | |
| TCAAATGCAATAGTTGCTTA+TGG | - | chr4.1:37592919-37592938 | MS.gene67382:CDS | 30.0% | |
| TCACCATTGAATTTCAAAAC+TGG | - | chr4.1:37592675-37592694 | MS.gene67382:CDS | 30.0% | |
| TCAGGAAGTTCATAATTGTT+AGG | + | chr4.1:37593065-37593084 | None:intergenic | 30.0% | |
| TGTTGAAGATGCAAATGTTT+GGG | - | chr4.1:37592956-37592975 | MS.gene67382:CDS | 30.0% | |
| ! | TCCTTTGTTGGCAATATTTT+GGG | + | chr4.1:37592582-37592601 | None:intergenic | 30.0% |
| ! | TGTCATGTCTCATTTTGTAA+CGG | + | chr4.1:37592155-37592174 | None:intergenic | 30.0% |
| ! | TTCCTTTGTTGGCAATATTT+TGG | + | chr4.1:37592583-37592602 | None:intergenic | 30.0% |
| !!! | ACACCAGTTTTGAAATTCAA+TGG | + | chr4.1:37592681-37592700 | None:intergenic | 30.0% |
| AAAGCAGTGATTGATGGAAT+TGG | - | chr4.1:37592729-37592748 | MS.gene67382:CDS | 35.0% | |
| AAAGGATTGACCAAAGACTT+TGG | + | chr4.1:37593005-37593024 | None:intergenic | 35.0% | |
| AAGAACATCAATTTCGGAAG+GGG | + | chr4.1:37592076-37592095 | None:intergenic | 35.0% | |
| AATCCTTTCATGGAGAAGTA+TGG | - | chr4.1:37593017-37593036 | MS.gene67382:CDS | 35.0% | |
| ACAAGAACATCAATTTCGGA+AGG | + | chr4.1:37592078-37592097 | None:intergenic | 35.0% | |
| ACCCAAAATATTGCCAACAA+AGG | - | chr4.1:37592578-37592597 | MS.gene67382:CDS | 35.0% | |
| CAAATCACAGAGAAACAAGT+TGG | - | chr4.1:37592248-37592267 | MS.gene67382:CDS | 35.0% | |
| CAACACAAGATAGAATGCTA+GGG | - | chr4.1:37591824-37591843 | MS.gene67382:CDS | 35.0% | |
| CAAGAACATCAATTTCGGAA+GGG | + | chr4.1:37592077-37592096 | None:intergenic | 35.0% | |
| GACGACAAGAACATCAATTT+CGG | + | chr4.1:37592082-37592101 | None:intergenic | 35.0% | |
| GATCGTATAGTTTCCAGATA+AGG | + | chr4.1:37591756-37591775 | None:intergenic | 35.0% | |
| GCATTGCAAAACTTCTATCA+AGG | - | chr4.1:37592040-37592059 | MS.gene67382:CDS | 35.0% | |
| GGAAACTATACGATCAAAAG+AGG | - | chr4.1:37591761-37591780 | MS.gene67382:CDS | 35.0% | |
| GTTTCCAGATAAGGAAACAT+AGG | + | chr4.1:37591747-37591766 | None:intergenic | 35.0% | |
| TCATTGCCAGTATACCAATT+TGG | + | chr4.1:37592303-37592322 | None:intergenic | 35.0% | |
| TGCCAACAAAGGAAATACTT+AGG | - | chr4.1:37592589-37592608 | MS.gene67382:CDS | 35.0% | |
| TGTACCTATGTTTCCTTATC+TGG | - | chr4.1:37591740-37591759 | MS.gene67382:CDS | 35.0% | |
| ! | AGAATGACACTACATCGTTT+TGG | - | chr4.1:37592792-37592811 | MS.gene67382:CDS | 35.0% |
| ! | TTTATGATAAGCTTTGGTGC+TGG | - | chr4.1:37592894-37592913 | MS.gene67382:CDS | 35.0% |
| ! | TTTCATGGAGAAGTATGGTT+GGG | - | chr4.1:37593022-37593041 | MS.gene67382:CDS | 35.0% |
| !!! | AAAGCTTTTGTTGCTGCTTT+TGG | + | chr4.1:37592534-37592553 | None:intergenic | 35.0% |
| ACTCAAAAGGAAGATGAGCA+GGG | - | chr4.1:37592477-37592496 | MS.gene67382:CDS | 40.0% | |
| AGGAAACATAGGTACAATGC+TGG | + | chr4.1:37591736-37591755 | None:intergenic | 40.0% | |
| ATGATTCGCGACGATAAAGA+AGG | + | chr4.1:37592129-37592148 | None:intergenic | 40.0% | |
| CAACCATACTTCTCCATGAA+AGG | + | chr4.1:37593023-37593042 | None:intergenic | 40.0% | |
| CCAACACAAGATAGAATGCT+AGG | - | chr4.1:37591823-37591842 | MS.gene67382:CDS | 40.0% | |
| CCTAGCATTCTATCTTGTGT+TGG | + | chr4.1:37591826-37591845 | None:intergenic | 40.0% | |
| CGACGATAAAGAAGGAAGAA+CGG | + | chr4.1:37592121-37592140 | None:intergenic | 40.0% | |
| CTCAAAGCCATTGTAAGTTG+TGG | - | chr4.1:37591913-37591932 | MS.gene67382:CDS | 40.0% | |
| CTCTGTCCAAATTGGTATAC+TGG | - | chr4.1:37592294-37592313 | MS.gene67382:CDS | 40.0% | |
| GGTACATGGAAGCAAAAAAG+AGG | - | chr4.1:37592850-37592869 | MS.gene67382:CDS | 40.0% | |
| GTCTTTGGTCAATCCTTTCA+TGG | - | chr4.1:37593007-37593026 | MS.gene67382:CDS | 40.0% | |
| ! | AGTAGCTTGTGGTATGTGTT+AGG | - | chr4.1:37592828-37592847 | MS.gene67382:CDS | 40.0% |
| ! | CACAACTTACAATGGCTTTG+AGG | + | chr4.1:37591915-37591934 | None:intergenic | 40.0% |
| ! | CTTTCATGGAGAAGTATGGT+TGG | - | chr4.1:37593021-37593040 | MS.gene67382:CDS | 40.0% |
| ! | GTAGCTTGTGGTATGTGTTA+GGG | - | chr4.1:37592829-37592848 | MS.gene67382:CDS | 40.0% |
| ! | TTACACTTGCTGCACTCAAA+AGG | - | chr4.1:37592464-37592483 | MS.gene67382:CDS | 40.0% |
| !! | CTTGTGTTGGCTTGTAACAT+TGG | + | chr4.1:37591813-37591832 | None:intergenic | 40.0% |
| !! | GAGAAACAAGTTGGCACTTT+TGG | - | chr4.1:37592257-37592276 | MS.gene67382:CDS | 40.0% |
| !! | GATCATTTTTGTCTCCACAC+AGG | - | chr4.1:37592702-37592721 | MS.gene67382:CDS | 40.0% |
| AAAAGGAAGATGAGCAGGGT+AGG | - | chr4.1:37592481-37592500 | MS.gene67382:CDS | 45.0% | |
| ACACTTCTGCTAGTAGCTTG+TGG | - | chr4.1:37592817-37592836 | MS.gene67382:CDS | 45.0% | |
| AGTGTACAATCTCACCGCAA+TGG | - | chr4.1:37592179-37592198 | MS.gene67382:CDS | 45.0% | |
| CACTCAAAAGGAAGATGAGC+AGG | - | chr4.1:37592476-37592495 | MS.gene67382:CDS | 45.0% | |
| CAGAGTCTCTCTGTCCAAAT+TGG | - | chr4.1:37592286-37592305 | MS.gene67382:CDS | 45.0% | |
| GCAAGTGTAAGAGTCATCTC+TGG | + | chr4.1:37592454-37592473 | None:intergenic | 45.0% | |
| GCCAATGCCACAACTTACAA+TGG | + | chr4.1:37591923-37591942 | None:intergenic | 45.0% | |
| GCCATTGTAAGTTGTGGCAT+TGG | - | chr4.1:37591919-37591938 | MS.gene67382:CDS | 45.0% | |
| GCCTTCAAAGACGTTTCTTG+GGG | + | chr4.1:37591959-37591978 | None:intergenic | 45.0% | |
| GGAGGAAAAGCAGTGATTGA+TGG | - | chr4.1:37592723-37592742 | MS.gene67382:CDS | 45.0% | |
| GGATTGACCAAAGACTTTGG+TGG | + | chr4.1:37593002-37593021 | None:intergenic | 45.0% | |
| GGCCTTCAAAGACGTTTCTT+GGG | + | chr4.1:37591960-37591979 | None:intergenic | 45.0% | |
| GTGGTATGTGTTAGGGTACA+TGG | - | chr4.1:37592836-37592855 | MS.gene67382:CDS | 45.0% | |
| GTGTACAATCTCACCGCAAT+GGG | - | chr4.1:37592180-37592199 | MS.gene67382:CDS | 45.0% | |
| TCCAGAACGGAAAAGACAGT+TGG | + | chr4.1:37592343-37592362 | None:intergenic | 45.0% | |
| TGGTTGGGTCAATGAAGTTG+AGG | - | chr4.1:37593037-37593056 | MS.gene67382:CDS | 45.0% | |
| TGTTTAGTACGAACGCACCA+CGG | - | chr4.1:37592426-37592445 | MS.gene67382:CDS | 45.0% | |
| ! | AACTGTCTTTTCCGTTCTGG+AGG | - | chr4.1:37592342-37592361 | MS.gene67382:CDS | 45.0% |
| ! | CATTTTTGTCTCCACACAGG+AGG | - | chr4.1:37592705-37592724 | MS.gene67382:CDS | 45.0% |
| ! | CGATGTAGTGTCATTCTTGC+AGG | + | chr4.1:37592789-37592808 | None:intergenic | 45.0% |
| !! | GTAGGCTTGGTGTTCACTTA+GGG | - | chr4.1:37592499-37592518 | MS.gene67382:CDS | 45.0% |
| ACTAGCACTGCAACCCATTG+CGG | + | chr4.1:37592196-37592215 | None:intergenic | 50.0% | |
| AGAAGGAAGAACGGAGAGCA+TGG | + | chr4.1:37592112-37592131 | None:intergenic | 50.0% | |
| AGATCCCGTCGTTCAGTGTT+GGG | + | chr4.1:37591993-37592012 | None:intergenic | 50.0% | |
| ATGCTAGGGACAGAGTTCTG+TGG | - | chr4.1:37591838-37591857 | MS.gene67382:CDS | 50.0% | |
| CGGCCTTCAAAGACGTTTCT+TGG | + | chr4.1:37591961-37591980 | None:intergenic | 50.0% | |
| GCCCCAAGAAACGTCTTTGA+AGG | - | chr4.1:37591955-37591974 | MS.gene67382:CDS | 50.0% | |
| ! | GCCAACTGTCTTTTCCGTTC+TGG | - | chr4.1:37592339-37592358 | MS.gene67382:CDS | 50.0% |
| !! | GGTAGGCTTGGTGTTCACTT+AGG | - | chr4.1:37592498-37592517 | MS.gene67382:CDS | 50.0% |
| !! | TCACTGCTTTTCCTCCTGTG+TGG | + | chr4.1:37592719-37592738 | None:intergenic | 50.0% |
| GAAGATGAGCAGGGTAGGCT+TGG | - | chr4.1:37592486-37592505 | MS.gene67382:CDS | 55.0% | |
| GAGATCCCGTCGTTCAGTGT+TGG | + | chr4.1:37591994-37592013 | None:intergenic | 55.0% | |
| GATCCCCAACACTGAACGAC+GGG | - | chr4.1:37591986-37592005 | MS.gene67382:CDS | 55.0% | |
| GATCCCGTCGTTCAGTGTTG+GGG | + | chr4.1:37591992-37592011 | None:intergenic | 55.0% | |
| GGATCCCCAACACTGAACGA+CGG | - | chr4.1:37591985-37592004 | MS.gene67382:CDS | 55.0% | |
| ! | AACGTCTTTGAAGGCCGCGA+AGG | - | chr4.1:37591964-37591983 | MS.gene67382:CDS | 55.0% |
| ! | TCAGTGTTGGGGATCCTTCG+CGG | + | chr4.1:37591981-37592000 | None:intergenic | 55.0% |
| !! | TTTCCGTTCTGGAGGATGCG+CGG | - | chr4.1:37592350-37592369 | MS.gene67382:CDS | 55.0% |
| AAGAGTCATCTCTGGCGCCG+TGG | + | chr4.1:37592446-37592465 | None:intergenic | 60.0% | |
| GAACCGCGCATCCTCCAGAA+CGG | + | chr4.1:37592356-37592375 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 37591703 | 37593097 | 37591703 | ID=MS.gene67382 |
| chr4.1 | mRNA | 37591703 | 37593097 | 37591703 | ID=MS.gene67382.t1;Parent=MS.gene67382 |
| chr4.1 | exon | 37591703 | 37593097 | 37591703 | ID=MS.gene67382.t1.exon1;Parent=MS.gene67382.t1 |
| chr4.1 | CDS | 37591703 | 37593097 | 37591703 | ID=cds.MS.gene67382.t1;Parent=MS.gene67382.t1 |
| Gene Sequence |
| Protein sequence |
