AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74869


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74869.t1 MTR_1g079270 80.000 50 10 0 34 83 213 262 9.74e-21 87.4
MS.gene74869.t1 MTR_1g079270 84.444 45 7 0 80 124 328 372 1.37e-19 84.3
MS.gene74869.t1 MTR_6g016785 57.692 52 22 0 32 83 458 509 1.41e-13 67.8
MS.gene74869.t1 MTR_7g013170 58.000 50 21 0 34 83 394 443 2.02e-13 67.4
MS.gene74869.t1 MTR_6g016795 57.692 52 22 0 32 83 394 445 2.35e-13 67.0
MS.gene74869.t1 MTR_6g016795 50.000 58 21 1 80 137 511 560 4.78e-11 60.5
MS.gene74869.t1 MTR_6g016800 56.000 50 22 0 34 83 396 445 2.60e-13 67.0
MS.gene74869.t1 MTR_7g013810 39.773 88 35 1 34 121 391 460 4.85e-13 66.2
MS.gene74869.t1 MTR_7g028445 58.824 51 20 1 34 83 359 409 6.51e-13 65.9
MS.gene74869.t1 MTR_7g028445 48.276 58 22 1 80 137 475 524 6.32e-11 60.1
MS.gene74869.t1 MTR_4g036905 58.824 51 20 1 34 83 359 409 6.51e-13 65.9
MS.gene74869.t1 MTR_4g036905 48.276 58 22 1 80 137 475 524 6.32e-11 60.1
MS.gene74869.t1 MTR_6g016845 52.000 50 24 0 34 83 409 458 8.98e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74869.t1 AT5G15870 54.000 50 23 0 34 83 440 489 5.80e-11 60.5

Find 40 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGGTTGGGACTGCTTGGTT+TGG 0.247623 1.1:+58927628 MS.gene74869:CDS
GGGTTGGGACTGCTTGGTTT+GGG 0.255874 1.1:+58927629 MS.gene74869:CDS
TGTAAACATTGATCCATATT+GGG 0.256708 1.1:+58927255 MS.gene74869:CDS
TTGTAAACATTGATCCATAT+TGG 0.286933 1.1:+58927254 MS.gene74869:CDS
CACTCGAGATATTTCCTTGA+TGG 0.293905 1.1:+58927220 MS.gene74869:CDS
CGCTTGATAGGTTTAGAATA+TGG 0.329607 1.1:+58927377 MS.gene74869:intron
TTATACTTCTTACCCCAATA+TGG 0.333060 1.1:-58927268 None:intergenic
AATACTTTCCAAAGCTGTTA+AGG 0.338125 1.1:-58927429 None:intergenic
AAACCAGGGTTTGCTTATCT+TGG 0.353049 1.1:+58927509 MS.gene74869:CDS
TGGAGCAACCTTAACAGCTT+TGG 0.376313 1.1:+58927421 MS.gene74869:CDS
GGGACTGCTTGGTTTGGGGA+AGG 0.383767 1.1:+58927634 MS.gene74869:CDS
AGGAAATATCTCGAGTGATA+TGG 0.399023 1.1:-58927214 None:intergenic
TTGTGATAAGGAAGGTGCTT+TGG 0.403486 1.1:+58927690 MS.gene74869:CDS
TCTAGTGGTGAAGCTGTGTT+TGG 0.405663 1.1:+58926642 MS.gene74869:CDS
GTGAAGGAACTTGTCGGGTT+GGG 0.438392 1.1:+58927614 MS.gene74869:intron
CTTGTCGGGTTGGGACTGCT+TGG 0.449692 1.1:+58927623 MS.gene74869:CDS
GAGTAGACCTTGGAGAAGAA+AGG 0.453767 1.1:+58926668 MS.gene74869:CDS
TCTGACTCTAGTTATGACTT+TGG 0.479169 1.1:+58927169 MS.gene74869:CDS
TTTGTAGAGAATTTGTGATA+AGG 0.486578 1.1:+58927678 MS.gene74869:CDS
AGAAGGAATGAATATGTATG+AGG 0.491603 1.1:+58927478 MS.gene74869:CDS
AGTAGACCTTGGAGAAGAAA+GGG 0.502514 1.1:+58926669 MS.gene74869:CDS
TAGAGAATTTGTGATAAGGA+AGG 0.511186 1.1:+58927682 MS.gene74869:CDS
GACGCCAATGTTGTTACTGT+TGG 0.529324 1.1:+58927401 MS.gene74869:CDS
ACCGAACCCTTTCTTCTCCA+AGG 0.541202 1.1:-58926675 None:intergenic
TGTGAAGGAACTTGTCGGGT+TGG 0.546197 1.1:+58927613 MS.gene74869:intron
TTTATATGATCAGGAATATG+GGG 0.551445 1.1:+58927121 MS.gene74869:intron
GTTTGTTACACTGTGTCTAG+TGG 0.556639 1.1:+58926627 MS.gene74869:CDS
AATATGTGAAGGAACTTGTC+GGG 0.559686 1.1:+58927609 MS.gene74869:intron
GGTTGGGACTGCTTGGTTTG+GGG 0.562711 1.1:+58927630 MS.gene74869:CDS
GTAAACATTGATCCATATTG+GGG 0.577879 1.1:+58927256 MS.gene74869:CDS
GAATATGTGAAGGAACTTGT+CGG 0.578775 1.1:+58927608 MS.gene74869:intron
CAAGAACAGCAATACCATCA+AGG 0.580016 1.1:-58927234 None:intergenic
TGTTTGGATTGAGTAGACCT+TGG 0.601321 1.1:+58926658 MS.gene74869:CDS
ATGTGGTGGCATGTAAGAGA+AGG 0.604058 1.1:+58927461 MS.gene74869:CDS
GGAGGAGTTGTTACAAAACA+AGG 0.618888 1.1:+58927142 MS.gene74869:CDS
ATATGATCAGGAATATGGGG+AGG 0.640187 1.1:+58927124 MS.gene74869:intron
TGCTCCAACAGTAACAACAT+TGG 0.651685 1.1:-58927405 None:intergenic
AAAGTATTAGAGCTCAAATG+TGG 0.658452 1.1:+58927444 MS.gene74869:CDS
GTATTAGAGCTCAAATGTGG+TGG 0.662975 1.1:+58927447 MS.gene74869:CDS
GCACCAAGATAAGCAAACCC+TGG 0.666993 1.1:-58927512 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATATTATGAAATTGTTT+CGG + chr1.1:58926902-58926921 MS.gene74869:intron 10.0%
!!! AAAACAAACACTTTTGAAAT+AGG - chr1.1:58927580-58927599 None:intergenic 20.0%
!!! AAATGGATTTTTATATGATC+AGG + chr1.1:58927112-58927131 MS.gene74869:intron 20.0%
!!! TTTTATATGATCAGGAATAT+GGG + chr1.1:58927120-58927139 MS.gene74869:intron 20.0%
!!! TTTTTATATGATCAGGAATA+TGG + chr1.1:58927119-58927138 MS.gene74869:intron 20.0%
! TGTAAACATTGATCCATATT+GGG + chr1.1:58927255-58927274 MS.gene74869:CDS 25.0%
! TTGTAAACATTGATCCATAT+TGG + chr1.1:58927254-58927273 MS.gene74869:CDS 25.0%
! TTTATATGATCAGGAATATG+GGG + chr1.1:58927121-58927140 MS.gene74869:intron 25.0%
! TTTATGGATAATTGATTGCA+AGG + chr1.1:58926985-58927004 MS.gene74869:intron 25.0%
! TTTGTAGAGAATTTGTGATA+AGG + chr1.1:58927678-58927697 MS.gene74869:CDS 25.0%
!! TAAATTCATCTGAGATAACA+AGG + chr1.1:58926949-58926968 MS.gene74869:intron 25.0%
!! TTCAAATACTTCCTAACTTT+TGG - chr1.1:58927053-58927072 None:intergenic 25.0%
!!! AGGACTTCATCTTATTTTTA+TGG + chr1.1:58926969-58926988 MS.gene74869:intron 25.0%
AATACTTTCCAAAGCTGTTA+AGG - chr1.1:58927432-58927451 None:intergenic 30.0%
AGAAGGAATGAATATGTATG+AGG + chr1.1:58927478-58927497 MS.gene74869:CDS 30.0%
AGTATGCATTAACAACTTCA+CGG - chr1.1:58927349-58927368 None:intergenic 30.0%
GATGGAACTTTCAAAGAAAA+TGG + chr1.1:58927095-58927114 MS.gene74869:intron 30.0%
GTAAACATTGATCCATATTG+GGG + chr1.1:58927256-58927275 MS.gene74869:CDS 30.0%
TAGAGAATTTGTGATAAGGA+AGG + chr1.1:58927682-58927701 MS.gene74869:CDS 30.0%
TTATACTTCTTACCCCAATA+TGG - chr1.1:58927271-58927290 None:intergenic 30.0%
TTCTGATGTTGAATATGTGA+AGG + chr1.1:58927598-58927617 MS.gene74869:intron 30.0%
TTGATGTGATTCCAAAAGTT+AGG + chr1.1:58927039-58927058 MS.gene74869:intron 30.0%
! AAAGTATTAGAGCTCAAATG+TGG + chr1.1:58927444-58927463 MS.gene74869:CDS 30.0%
! ATGAGGAAGATTTTAAACCA+GGG + chr1.1:58927495-58927514 MS.gene74869:CDS 30.0%
! TATGAGGAAGATTTTAAACC+AGG + chr1.1:58927494-58927513 MS.gene74869:CDS 30.0%
!!! ACTTTTGAAATAGGCAACAA+TGG - chr1.1:58927571-58927590 None:intergenic 30.0%
AATATGTGAAGGAACTTGTC+GGG + chr1.1:58927609-58927628 MS.gene74869:intron 35.0%
AGGAAATATCTCGAGTGATA+TGG - chr1.1:58927217-58927236 None:intergenic 35.0%
CCAAGTTACAAAAACAGGAT+CGG - chr1.1:58926856-58926875 None:intergenic 35.0%
GAATATGTGAAGGAACTTGT+CGG + chr1.1:58927608-58927627 MS.gene74869:intron 35.0%
GTATGCATTAACAACTTCAC+GGG - chr1.1:58927348-58927367 None:intergenic 35.0%
TCTGACTCTAGTTATGACTT+TGG + chr1.1:58927169-58927188 MS.gene74869:CDS 35.0%
TGAAAGAGATCATTGAGCTT+TGG + chr1.1:58927069-58927088 MS.gene74869:intron 35.0%
TGGATAATTGATTGCAAGGT+TGG + chr1.1:58926989-58927008 MS.gene74869:intron 35.0%
! CGCTTGATAGGTTTAGAATA+TGG + chr1.1:58927377-58927396 MS.gene74869:intron 35.0%
!! AGCATTGATAGTGAACTTGT+TGG + chr1.1:58926801-58926820 MS.gene74869:intron 35.0%
AGTAGACCTTGGAGAAGAAA+GGG + chr1.1:58926669-58926688 MS.gene74869:CDS 40.0%
ATATGATCAGGAATATGGGG+AGG + chr1.1:58927124-58927143 MS.gene74869:intron 40.0%
CAAGAACAGCAATACCATCA+AGG - chr1.1:58927237-58927256 None:intergenic 40.0%
CACTCGAGATATTTCCTTGA+TGG + chr1.1:58927220-58927239 MS.gene74869:CDS 40.0%
GAGTGCCAAGTTACAAAAAC+AGG - chr1.1:58926861-58926880 None:intergenic 40.0%
GGAGGAGTTGTTACAAAACA+AGG + chr1.1:58927142-58927161 MS.gene74869:CDS 40.0%
GTAACTTGGCACTCTATGAA+TGG + chr1.1:58926867-58926886 MS.gene74869:intron 40.0%
GTATTAGAGCTCAAATGTGG+TGG + chr1.1:58927447-58927466 MS.gene74869:CDS 40.0%
GTTCCTTTGAACGATGTTGA+GGG + chr1.1:58926924-58926943 MS.gene74869:intron 40.0%
TACTATTCTGCTCGCTTGAT+AGG + chr1.1:58927365-58927384 MS.gene74869:intron 40.0%
TGCTCCAACAGTAACAACAT+TGG - chr1.1:58927408-58927427 None:intergenic 40.0%
TGTTTGGATTGAGTAGACCT+TGG + chr1.1:58926658-58926677 MS.gene74869:CDS 40.0%
! AAACCAGGGTTTGCTTATCT+TGG + chr1.1:58927509-58927528 MS.gene74869:CDS 40.0%
! GCGTCAAACTTGTTCACTTA+TGG + chr1.1:58927291-58927310 MS.gene74869:intron 40.0%
! GTTTGTTACACTGTGTCTAG+TGG + chr1.1:58926627-58926646 MS.gene74869:CDS 40.0%
!! AGTGAACTTGTTGGTGTTGT+TGG + chr1.1:58926810-58926829 MS.gene74869:intron 40.0%
!! ATCATTGAGCTTTGGCTTGA+TGG + chr1.1:58927077-58927096 MS.gene74869:intron 40.0%
!! TTGGTGCTAGAGACAATGTA+TGG + chr1.1:58927528-58927547 MS.gene74869:intron 40.0%
!! TTGTGATAAGGAAGGTGCTT+TGG + chr1.1:58927690-58927709 MS.gene74869:CDS 40.0%
!!! CCGATCCTGTTTTTGTAACT+TGG + chr1.1:58926853-58926872 MS.gene74869:intron 40.0%
ACCTTGGAGAAGAAAGGGTT+CGG + chr1.1:58926674-58926693 MS.gene74869:intron 45.0%
AGTCCCTCAACATCGTTCAA+AGG - chr1.1:58926930-58926949 None:intergenic 45.0%
ATGTGGTGGCATGTAAGAGA+AGG + chr1.1:58927461-58927480 MS.gene74869:CDS 45.0%
GACGCCAATGTTGTTACTGT+TGG + chr1.1:58927401-58927420 MS.gene74869:CDS 45.0%
GAGTAGACCTTGGAGAAGAA+AGG + chr1.1:58926668-58926687 MS.gene74869:CDS 45.0%
GGTTCCTTTGAACGATGTTG+AGG + chr1.1:58926923-58926942 MS.gene74869:intron 45.0%
TCTAGTGGTGAAGCTGTGTT+TGG + chr1.1:58926642-58926661 MS.gene74869:CDS 45.0%
TGGAGCAACCTTAACAGCTT+TGG + chr1.1:58927421-58927440 MS.gene74869:CDS 45.0%
!! GGATCGGTTTTCAAAAGCCA+TGG - chr1.1:58926840-58926859 None:intergenic 45.0%
!! TTGGTGTTGTTGGTGATCCA+TGG + chr1.1:58926820-58926839 MS.gene74869:intron 45.0%
ACCGAACCCTTTCTTCTCCA+AGG - chr1.1:58926678-58926697 None:intergenic 50.0%
GCACCAAGATAAGCAAACCC+TGG - chr1.1:58927515-58927534 None:intergenic 50.0%
GTGAAGGAACTTGTCGGGTT+GGG + chr1.1:58927614-58927633 MS.gene74869:intron 50.0%
TGTGAAGGAACTTGTCGGGT+TGG + chr1.1:58927613-58927632 MS.gene74869:intron 50.0%
GGGTTGGGACTGCTTGGTTT+GGG + chr1.1:58927629-58927648 MS.gene74869:CDS 55.0%
! GGTTGGGACTGCTTGGTTTG+GGG + chr1.1:58927630-58927649 MS.gene74869:CDS 55.0%
! CGGGTTGGGACTGCTTGGTT+TGG + chr1.1:58927628-58927647 MS.gene74869:CDS 60.0%
!! CTTGTCGGGTTGGGACTGCT+TGG + chr1.1:58927623-58927642 MS.gene74869:CDS 60.0%
!! GGGACTGCTTGGTTTGGGGA+AGG + chr1.1:58927634-58927653 MS.gene74869:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 58926597 58927721 58926597 ID=MS.gene74869
chr1.1 mRNA 58926597 58927721 58926597 ID=MS.gene74869.t1;Parent=MS.gene74869
chr1.1 exon 58926597 58926690 58926597 ID=MS.gene74869.t1.exon1;Parent=MS.gene74869.t1
chr1.1 CDS 58926597 58926690 58926597 ID=cds.MS.gene74869.t1;Parent=MS.gene74869.t1
chr1.1 exon 58927134 58927277 58927134 ID=MS.gene74869.t1.exon2;Parent=MS.gene74869.t1
chr1.1 CDS 58927134 58927277 58927134 ID=cds.MS.gene74869.t1;Parent=MS.gene74869.t1
chr1.1 exon 58927387 58927544 58927387 ID=MS.gene74869.t1.exon3;Parent=MS.gene74869.t1
chr1.1 CDS 58927387 58927544 58927387 ID=cds.MS.gene74869.t1;Parent=MS.gene74869.t1
chr1.1 exon 58927620 58927721 58927620 ID=MS.gene74869.t1.exon4;Parent=MS.gene74869.t1
chr1.1 CDS 58927620 58927721 58927620 ID=cds.MS.gene74869.t1;Parent=MS.gene74869.t1
Gene Sequence

>MS.gene74869

ATGAGATTGTTCTTGACATGTTCAGTTCTTGTTTGTTACACTGTGTCTAGTGGTGAAGCTGTGTTTGGATTGAGTAGACCTTGGAGAAGAAAGGGTTCGGTGATTTGTTATTGTTAGCAGTAGCACACACGCTCTTCATCTTCAGCTTTTATTTGAGAATGACTGTAATAATGTTTTACTGTTTTGAATGTTTTTATTGATAAAAGCATTGATAGTGAACTTGTTGGTGTTGTTGGTGATCCATGGCTTTTGAAAACCGATCCTGTTTTTGTAACTTGGCACTCTATGAATGGTGTGAAAGAAGAATTATATTATGAAATTGTTTCGGTTCCTTTGAACGATGTTGAGGGACTAAATTCATCTGAGATAACAAGGACTTCATCTTATTTTTATGGATAATTGATTGCAAGGTTGGCTTTGATAGCTGAAGACGTTTGTTTCTTTGATGTGATTCCAAAAGTTAGGAAGTATTTGAAAGAGATCATTGAGCTTTGGCTTGATGGAACTTTCAAAGAAAATGGATTTTTATATGATCAGGAATATGGGGAGGAGTTGTTACAAAACAAGGATGTTCTGACTCTAGTTATGACTTTGGATCTGAATTTTATACTTCTCAACCATATCACTCGAGATATTTCCTTGATGGTATTGCTGTTCTTGTAAACATTGATCCATATTGGGGTAAGAAGTATAAGCGTCAAACTTGTTCACTTATGGAAGAGTTTATGAACTTGAGCACATGTTCAAACCCGTGAAGTTGTTAATGCATACTATTCTGCTCGCTTGATAGGTTTAGAATATGGTGACGCCAATGTTGTTACTGTTGGAGCAACCTTAACAGCTTTGGAAAGTATTAGAGCTCAAATGTGGTGGCATGTAAGAGAAGGAATGAATATGTATGAGGAAGATTTTAAACCAGGGTTTGCTTATCTTGGTGCTAGAGACAATGTATGGTTGACATTCAAGTTTTACCATTGTTGCCTATTTCAAAAGTGTTTGTTTTCTGATGTTGAATATGTGAAGGAACTTGTCGGGTTGGGACTGCTTGGTTTGGGGAAGGCTGAAAAGAGTTTGTGTATGCTTTGTAGAGAATTTGTGATAAGGAAGGTGCTTTGGAGAAGATAA

Protein sequence

>MS.gene74869.t1

MRLFLTCSVLVCYTVSSGEAVFGLSRPWRRKGIWGGVVTKQGCSDSSYDFGSEFYTSQPYHSRYFLDGIAVLVNIDPYWGLEYGDANVVTVGATLTALESIRAQMWWHVREGMNMYEEDFKPGFAYLGARDNELVGLGLLGLGKAEKSLCMLCREFVIRKVLWRR