Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81322.t1 | RHN58005.1 | 100 | 73 | 0 | 0 | 1 | 73 | 121 | 193 | 2.60E-31 | 144.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81322.t1 | Q9FM67 | 80.8 | 73 | 14 | 0 | 1 | 73 | 137 | 209 | 2.2e-26 | 119.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81322.t1 | G7KEF3 | 100.0 | 73 | 0 | 0 | 1 | 73 | 143 | 215 | 1.9e-31 | 144.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene81322 | 0.809799 | 1.58E-50 | -1.69E-46 |
| MS.gene049153 | MS.gene81322 | 0.805321 | 1.41E-49 | -1.69E-46 |
| MS.gene049230 | MS.gene81322 | 0.800012 | 1.76E-48 | -1.69E-46 |
| MS.gene049260 | MS.gene81322 | 0.817935 | 2.55E-52 | -1.69E-46 |
| MS.gene049297 | MS.gene81322 | 0.811854 | 5.68E-51 | -1.69E-46 |
| MS.gene049378 | MS.gene81322 | 0.823523 | 1.32E-53 | -1.69E-46 |
| MS.gene049389 | MS.gene81322 | 0.802288 | 6.02E-49 | -1.69E-46 |
| MS.gene049599 | MS.gene81322 | -0.810778 | 9.73E-51 | -1.69E-46 |
| MS.gene049758 | MS.gene81322 | 0.88558 | 7.19E-72 | -1.69E-46 |
| MS.gene049910 | MS.gene81322 | 0.850559 | 1.57E-60 | -1.69E-46 |
| MS.gene049915 | MS.gene81322 | 0.832344 | 9.98E-56 | -1.69E-46 |
| MS.gene050128 | MS.gene81322 | 0.83138 | 1.72E-55 | -1.69E-46 |
| MS.gene050226 | MS.gene81322 | 0.823141 | 1.62E-53 | -1.69E-46 |
| MS.gene050227 | MS.gene81322 | 0.817698 | 2.88E-52 | -1.69E-46 |
| MS.gene050228 | MS.gene81322 | 0.819469 | 1.14E-52 | -1.69E-46 |
| MS.gene050301 | MS.gene81322 | 0.846854 | 1.67E-59 | -1.69E-46 |
| MS.gene050330 | MS.gene81322 | -0.800977 | 1.12E-48 | -1.69E-46 |
| MS.gene050406 | MS.gene81322 | 0.809641 | 1.71E-50 | -1.69E-46 |
| MS.gene050464 | MS.gene81322 | 0.834621 | 2.70E-56 | -1.69E-46 |
| MS.gene050529 | MS.gene81322 | 0.836627 | 8.37E-57 | -1.69E-46 |
| MS.gene050558 | MS.gene81322 | 0.801898 | 7.24E-49 | -1.69E-46 |
| MS.gene050623 | MS.gene81322 | -0.858835 | 6.27E-63 | -1.69E-46 |
| MS.gene050642 | MS.gene81322 | 0.879986 | 7.96E-70 | -1.69E-46 |
| MS.gene050670 | MS.gene81322 | 0.802103 | 6.58E-49 | -1.69E-46 |
| MS.gene05119 | MS.gene81322 | -0.819897 | 9.12E-53 | -1.69E-46 |
| MS.gene051471 | MS.gene81322 | 0.817724 | 2.84E-52 | -1.69E-46 |
| MS.gene051535 | MS.gene81322 | 0.807601 | 4.67E-50 | -1.69E-46 |
| MS.gene051662 | MS.gene81322 | 0.8314 | 1.71E-55 | -1.69E-46 |
| MS.gene051663 | MS.gene81322 | 0.821507 | 3.89E-53 | -1.69E-46 |
| MS.gene051860 | MS.gene81322 | 0.835304 | 1.81E-56 | -1.69E-46 |
| MS.gene051983 | MS.gene81322 | 0.87468 | 5.59E-68 | -1.69E-46 |
| MS.gene052077 | MS.gene81322 | 0.856644 | 2.80E-62 | -1.69E-46 |
| MS.gene052078 | MS.gene81322 | 0.834892 | 2.30E-56 | -1.69E-46 |
| MS.gene052176 | MS.gene81322 | 0.818388 | 2.01E-52 | -1.69E-46 |
| MS.gene052178 | MS.gene81322 | 0.831309 | 1.80E-55 | -1.69E-46 |
| MS.gene052188 | MS.gene81322 | 0.855872 | 4.71E-62 | -1.69E-46 |
| MS.gene052302 | MS.gene81322 | 0.802399 | 5.72E-49 | -1.69E-46 |
| MS.gene052309 | MS.gene81322 | -0.850393 | 1.75E-60 | -1.69E-46 |
| MS.gene052310 | MS.gene81322 | 0.804669 | 1.93E-49 | -1.69E-46 |
| MS.gene052340 | MS.gene81322 | 0.853346 | 2.54E-61 | -1.69E-46 |
| MS.gene052376 | MS.gene81322 | 0.809549 | 1.79E-50 | -1.69E-46 |
| MS.gene053073 | MS.gene81322 | 0.812541 | 4.03E-51 | -1.69E-46 |
| MS.gene05316 | MS.gene81322 | 0.80713 | 5.87E-50 | -1.69E-46 |
| MS.gene053557 | MS.gene81322 | 0.837795 | 4.21E-57 | -1.69E-46 |
| MS.gene053559 | MS.gene81322 | 0.845222 | 4.65E-59 | -1.69E-46 |
| MS.gene053836 | MS.gene81322 | 0.893608 | 5.36E-75 | -1.69E-46 |
| MS.gene053907 | MS.gene81322 | 0.839025 | 2.03E-57 | -1.69E-46 |
| MS.gene054349 | MS.gene81322 | 0.819501 | 1.12E-52 | -1.69E-46 |
| MS.gene054473 | MS.gene81322 | 0.817795 | 2.74E-52 | -1.69E-46 |
| MS.gene054542 | MS.gene81322 | 0.803079 | 4.14E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene81322 | 0.879713 | 9.95E-70 | -1.69E-46 |
| MS.gene054942 | MS.gene81322 | 0.819615 | 1.06E-52 | -1.69E-46 |
| MS.gene054983 | MS.gene81322 | 0.843159 | 1.66E-58 | -1.69E-46 |
| MS.gene055116 | MS.gene81322 | 0.801372 | 9.29E-49 | -1.69E-46 |
| MS.gene055422 | MS.gene81322 | 0.868518 | 6.15E-66 | -1.69E-46 |
| MS.gene055428 | MS.gene81322 | 0.835581 | 1.54E-56 | -1.69E-46 |
| MS.gene05553 | MS.gene81322 | 0.820602 | 6.29E-53 | -1.69E-46 |
| MS.gene055712 | MS.gene81322 | 0.836962 | 6.88E-57 | -1.69E-46 |
| MS.gene056069 | MS.gene81322 | -0.801062 | 1.08E-48 | -1.69E-46 |
| MS.gene056407 | MS.gene81322 | 0.801916 | 7.18E-49 | -1.69E-46 |
| MS.gene056612 | MS.gene81322 | 0.847146 | 1.39E-59 | -1.69E-46 |
| MS.gene056659 | MS.gene81322 | 0.813243 | 2.82E-51 | -1.69E-46 |
| MS.gene056899 | MS.gene81322 | -0.80728 | 5.46E-50 | -1.69E-46 |
| MS.gene056979 | MS.gene81322 | 0.873856 | 1.06E-67 | -1.69E-46 |
| MS.gene05702 | MS.gene81322 | 0.816682 | 4.88E-52 | -1.69E-46 |
| MS.gene057121 | MS.gene81322 | 0.826296 | 2.93E-54 | -1.69E-46 |
| MS.gene057439 | MS.gene81322 | 0.854509 | 1.17E-61 | -1.69E-46 |
| MS.gene057448 | MS.gene81322 | 0.819806 | 9.57E-53 | -1.69E-46 |
| MS.gene057449 | MS.gene81322 | 0.800801 | 1.22E-48 | -1.69E-46 |
| MS.gene057450 | MS.gene81322 | 0.829165 | 6.00E-55 | -1.69E-46 |
| MS.gene057754 | MS.gene81322 | 0.824889 | 6.32E-54 | -1.69E-46 |
| MS.gene058002 | MS.gene81322 | 0.858507 | 7.86E-63 | -1.69E-46 |
| MS.gene058082 | MS.gene81322 | 0.822068 | 2.89E-53 | -1.69E-46 |
| MS.gene05811 | MS.gene81322 | 0.837831 | 4.12E-57 | -1.69E-46 |
| MS.gene058142 | MS.gene81322 | 0.860354 | 2.19E-63 | -1.69E-46 |
| MS.gene058276 | MS.gene81322 | 0.821503 | 3.90E-53 | -1.69E-46 |
| MS.gene058433 | MS.gene81322 | 0.802369 | 5.80E-49 | -1.69E-46 |
| MS.gene058435 | MS.gene81322 | 0.823462 | 1.37E-53 | -1.69E-46 |
| MS.gene058661 | MS.gene81322 | -0.810364 | 1.20E-50 | -1.69E-46 |
| MS.gene058710 | MS.gene81322 | 0.823234 | 1.55E-53 | -1.69E-46 |
| MS.gene058749 | MS.gene81322 | 0.837048 | 6.54E-57 | -1.69E-46 |
| MS.gene058833 | MS.gene81322 | 0.810088 | 1.37E-50 | -1.69E-46 |
| MS.gene058845 | MS.gene81322 | 0.83571 | 1.43E-56 | -1.69E-46 |
| MS.gene05907 | MS.gene81322 | 0.840974 | 6.29E-58 | -1.69E-46 |
| MS.gene059285 | MS.gene81322 | 0.86631 | 3.13E-65 | -1.69E-46 |
| MS.gene059518 | MS.gene81322 | 0.844519 | 7.19E-59 | -1.69E-46 |
| MS.gene059597 | MS.gene81322 | 0.843526 | 1.33E-58 | -1.69E-46 |
| MS.gene059676 | MS.gene81322 | 0.808408 | 3.14E-50 | -1.69E-46 |
| MS.gene059692 | MS.gene81322 | 0.815916 | 7.24E-52 | -1.69E-46 |
| MS.gene059695 | MS.gene81322 | 0.808629 | 2.82E-50 | -1.69E-46 |
| MS.gene05984 | MS.gene81322 | 0.872913 | 2.21E-67 | -1.69E-46 |
| MS.gene059894 | MS.gene81322 | 0.869071 | 4.07E-66 | -1.69E-46 |
| MS.gene059899 | MS.gene81322 | 0.884712 | 1.52E-71 | -1.69E-46 |
| MS.gene060407 | MS.gene81322 | 0.811477 | 6.86E-51 | -1.69E-46 |
| MS.gene060476 | MS.gene81322 | 0.807736 | 4.37E-50 | -1.69E-46 |
| MS.gene060495 | MS.gene81322 | 0.81322 | 2.86E-51 | -1.69E-46 |
| MS.gene061049 | MS.gene81322 | 0.882692 | 8.41E-71 | -1.69E-46 |
| MS.gene061159 | MS.gene81322 | 0.805247 | 1.46E-49 | -1.69E-46 |
| MS.gene061265 | MS.gene81322 | 0.802169 | 6.37E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81322.t1 | MTR_5g095770 | 100.000 | 73 | 0 | 0 | 1 | 73 | 143 | 215 | 5.77e-46 | 145 |
| MS.gene81322.t1 | MTR_7g080300 | 47.945 | 73 | 37 | 1 | 1 | 72 | 137 | 209 | 2.33e-17 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81322.t1 | AT5G55710 | 80.822 | 73 | 14 | 0 | 1 | 73 | 137 | 209 | 3.01e-35 | 118 |
| MS.gene81322.t1 | AT2G47840 | 48.649 | 74 | 36 | 1 | 1 | 72 | 135 | 208 | 8.18e-16 | 68.6 |
Find 13 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGCTCTTCTTCTTGCATTT+TGG | 0.091593 | 5.2:+80509813 | MS.gene81322:CDS |
| TTGTTGGTGTGTTTGATTTA+TGG | 0.141836 | 5.2:+80509793 | MS.gene81322:CDS |
| ATTGGATTTGTTGATGAGTT+TGG | 0.285770 | 5.2:+80509750 | MS.gene81322:CDS |
| TTTAATCCAAGAGGTGGGTT+TGG | 0.383345 | 5.2:+80509727 | MS.gene81322:CDS |
| AACCCTCTTTCCAAAAGATC+AGG | 0.429515 | 5.2:-80509706 | None:intergenic |
| TCCAATCCAAACCCACCTCT+TGG | 0.441020 | 5.2:-80509733 | None:intergenic |
| TCCAAGAGGTGGGTTTGGAT+TGG | 0.441807 | 5.2:+80509732 | MS.gene81322:CDS |
| TCAGCAGCATCAGCAACAAT+AGG | 0.464175 | 5.2:-80509853 | None:intergenic |
| TTGTTGCTGATGCTGCTGAT+AGG | 0.467577 | 5.2:+80509857 | MS.gene81322:CDS |
| AGAGGGTTTAATCCAAGAGG+TGG | 0.570836 | 5.2:+80509721 | MS.gene81322:CDS |
| GAGGGTTTAATCCAAGAGGT+GGG | 0.602525 | 5.2:+80509722 | MS.gene81322:CDS |
| TCAGCAACAATAGGCAACCT+AGG | 0.619404 | 5.2:-80509844 | None:intergenic |
| GAAAGAGGGTTTAATCCAAG+AGG | 0.654426 | 5.2:+80509718 | MS.gene81322:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTGTTGGTGTGTTTGATTTA+TGG | + | chr5.2:80509793-80509812 | MS.gene81322:CDS | 30.0% |
| !! | ATTGGATTTGTTGATGAGTT+TGG | + | chr5.2:80509750-80509769 | MS.gene81322:CDS | 30.0% |
| !! | GTTGATTTTTCCTGATCTTT+TGG | + | chr5.2:80509696-80509715 | MS.gene81322:CDS | 30.0% |
| !! | CACTGTGTTTTTGTTCTTGT+TGG | + | chr5.2:80509777-80509796 | MS.gene81322:CDS | 35.0% |
| !! | TTCCTGATCTTTTGGAAAGA+GGG | + | chr5.2:80509704-80509723 | MS.gene81322:CDS | 35.0% |
| !! | TTTCCTGATCTTTTGGAAAG+AGG | + | chr5.2:80509703-80509722 | MS.gene81322:CDS | 35.0% |
| AACCCTCTTTCCAAAAGATC+AGG | - | chr5.2:80509709-80509728 | None:intergenic | 40.0% | |
| GAAAGAGGGTTTAATCCAAG+AGG | + | chr5.2:80509718-80509737 | MS.gene81322:CDS | 40.0% | |
| ! | GGCTCTTCTTCTTGCATTTT+GGG | + | chr5.2:80509814-80509833 | MS.gene81322:CDS | 40.0% |
| ! | TGGCTCTTCTTCTTGCATTT+TGG | + | chr5.2:80509813-80509832 | MS.gene81322:CDS | 40.0% |
| ! | TTTAATCCAAGAGGTGGGTT+TGG | + | chr5.2:80509727-80509746 | MS.gene81322:CDS | 40.0% |
| AGAGGGTTTAATCCAAGAGG+TGG | + | chr5.2:80509721-80509740 | MS.gene81322:CDS | 45.0% | |
| GAGGGTTTAATCCAAGAGGT+GGG | + | chr5.2:80509722-80509741 | MS.gene81322:CDS | 45.0% | |
| TCAGCAACAATAGGCAACCT+AGG | - | chr5.2:80509847-80509866 | None:intergenic | 45.0% | |
| TCAGCAGCATCAGCAACAAT+AGG | - | chr5.2:80509856-80509875 | None:intergenic | 45.0% | |
| TTGTTGCTGATGCTGCTGAT+AGG | + | chr5.2:80509857-80509876 | MS.gene81322:CDS | 45.0% | |
| TCCAATCCAAACCCACCTCT+TGG | - | chr5.2:80509736-80509755 | None:intergenic | 50.0% | |
| ! | TCCAAGAGGTGGGTTTGGAT+TGG | + | chr5.2:80509732-80509751 | MS.gene81322:CDS | 50.0% |
| !! | GCATTTTGGGTCAGCTACCT+AGG | + | chr5.2:80509827-80509846 | MS.gene81322:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 80509670 | 80509891 | 80509670 | ID=MS.gene81322 |
| chr5.2 | mRNA | 80509670 | 80509891 | 80509670 | ID=MS.gene81322.t1;Parent=MS.gene81322 |
| chr5.2 | exon | 80509670 | 80509891 | 80509670 | ID=MS.gene81322.t1.exon1;Parent=MS.gene81322.t1 |
| chr5.2 | CDS | 80509670 | 80509891 | 80509670 | ID=cds.MS.gene81322.t1;Parent=MS.gene81322.t1 |
| Gene Sequence |
| Protein sequence |
