AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene91581


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91581.t1 MTR_7g015010 97.721 351 8 0 1 351 1 351 0.0 699
MS.gene91581.t1 MTR_6g015350 43.243 370 153 15 1 351 2 333 4.90e-63 204
MS.gene91581.t1 MTR_4g109660 61.345 119 43 2 1 118 1 117 4.35e-45 157
MS.gene91581.t1 MTR_3g026050 44.348 230 102 9 1 209 1 225 1.98e-44 157
MS.gene91581.t1 MTR_8g463380 61.842 76 29 0 51 126 48 123 1.67e-24 103
MS.gene91581.t1 MTR_4g079580 59.740 77 31 0 51 127 16 92 4.57e-24 101
MS.gene91581.t1 MTR_2g078200 63.014 73 27 0 51 123 14 86 6.55e-24 100
MS.gene91581.t1 MTR_2g090960 62.162 74 28 0 51 124 18 91 1.90e-23 99.4
MS.gene91581.t1 MTR_4g111935 29.856 278 155 11 18 264 90 358 1.43e-15 77.4
MS.gene91581.t1 MTR_6g017055 40.566 106 57 3 58 158 127 231 6.26e-14 72.8
MS.gene91581.t1 MTR_7g018500 52.632 57 27 0 59 115 122 178 2.86e-13 70.5
MS.gene91581.t1 MTR_1g103380 50.000 60 30 0 56 115 200 259 6.73e-12 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene91581.t1 AT3G02150 76.000 100 24 0 30 129 47 146 9.97e-46 157
MS.gene91581.t1 AT3G02150 76.000 100 24 0 30 129 47 146 2.30e-45 159
MS.gene91581.t1 AT5G08070 70.707 99 25 2 25 123 4 98 4.90e-42 147
MS.gene91581.t1 AT5G08070 70.707 99 25 2 25 123 4 98 4.90e-42 147
MS.gene91581.t1 AT5G60970 82.500 80 13 1 43 122 49 127 1.06e-41 149
MS.gene91581.t1 AT1G30210 73.611 72 19 0 47 118 40 111 1.44e-29 116
MS.gene91581.t1 AT1G30210 73.611 72 19 0 47 118 40 111 1.44e-29 116
MS.gene91581.t1 AT4G18390 35.385 260 111 8 47 298 32 242 1.87e-27 111
MS.gene91581.t1 AT4G18390 35.385 260 111 8 47 298 32 242 1.87e-27 111
MS.gene91581.t1 AT1G53230 63.158 76 28 0 51 126 43 118 1.39e-25 106
MS.gene91581.t1 AT1G53230 63.158 76 28 0 51 126 43 118 1.39e-25 106
MS.gene91581.t1 AT3G15030 62.162 74 28 0 51 124 39 112 7.11e-24 102
MS.gene91581.t1 AT3G15030 62.162 74 28 0 51 124 39 112 7.11e-24 102
MS.gene91581.t1 AT3G15030 62.162 74 28 0 51 124 39 112 7.11e-24 102
MS.gene91581.t1 AT3G15030 62.162 74 28 0 51 124 39 112 7.11e-24 102
MS.gene91581.t1 AT2G31070 66.154 65 22 0 51 115 23 87 3.48e-21 93.6
MS.gene91581.t1 AT1G67260 43.421 76 43 0 46 121 76 151 4.20e-15 75.9
MS.gene91581.t1 AT1G67260 43.421 76 43 0 46 121 76 151 4.20e-15 75.9
MS.gene91581.t1 AT1G67260 43.421 76 43 0 46 121 76 151 4.39e-15 75.9
MS.gene91581.t1 AT1G68800 47.761 67 35 0 49 115 104 170 6.03e-15 75.5
MS.gene91581.t1 AT1G68800 47.761 67 35 0 49 115 104 170 6.69e-15 75.5
MS.gene91581.t1 AT3G18550 44.048 84 41 2 32 115 129 206 1.34e-13 72.0
MS.gene91581.t1 AT3G18550 44.048 84 41 2 32 115 129 206 1.45e-13 71.6
MS.gene91581.t1 AT3G18550 44.048 84 41 2 32 115 129 206 1.45e-13 71.6
MS.gene91581.t1 AT3G18550 44.048 84 41 2 32 115 129 206 1.84e-13 71.6

Find 61 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAAATCACCCTTCATTTAT+TGG 0.099357 7.4:-84460546 MS.gene91581:CDS
TGTAACATATTGTTATTATT+TGG 0.154767 7.4:+84460573 None:intergenic
CTTCAAGTCAAAATTCTTCT+TGG 0.156785 7.4:-84460728 MS.gene91581:CDS
AGTGAACTCTTGTTCTTTAT+TGG 0.160335 7.4:+84460173 None:intergenic
AATTCTCTTGAAACTTCTTT+AGG 0.204373 7.4:+84460696 None:intergenic
GTTCCTACAGGTGGAAAATT+AGG 0.209351 7.4:+84460843 None:intergenic
CTTGATACTCTGACAATTCT+TGG 0.239929 7.4:+84461062 None:intergenic
GTACTATTCATCAAGTAATT+TGG 0.245121 7.4:+84460660 None:intergenic
ATTCTCTTGAAACTTCTTTA+GGG 0.288069 7.4:+84460697 None:intergenic
CCAATAAATGAAGGGTGATT+TGG 0.289695 7.4:+84460546 None:intergenic
ATTCTTGGATCTTTCAATCT+TGG 0.296306 7.4:+84461077 None:intergenic
TGCCGAAAATTCTTCTCAAT+TGG 0.305771 7.4:-84460466 MS.gene91581:CDS
GTCAGAGTATCAAGAGCTTT+TGG 0.318336 7.4:-84461053 MS.gene91581:CDS
TAAAGAAGTTTCAAGAGAAT+TGG 0.320601 7.4:-84460694 MS.gene91581:CDS
AATTCTTCTCAAATGATGTT+AGG 0.334825 7.4:-84460390 MS.gene91581:CDS
TCAATAGGAACAATATTCAT+TGG 0.339202 7.4:-84460761 MS.gene91581:CDS
TATGATCTTCAAGATAGGTT+AGG 0.344793 7.4:-84460942 MS.gene91581:CDS
CCAATGTTGTCTTTATCAAC+TGG 0.374206 7.4:-84460414 MS.gene91581:CDS
TGATGAACCTCTTGTTTCAA+TGG 0.375029 7.4:+84461170 None:intergenic
AACAATTGTTCAATTTCAAT+AGG 0.377158 7.4:-84460776 MS.gene91581:CDS
AACCTAGCAAAGTTGTTGAT+TGG 0.387978 7.4:-84460911 MS.gene91581:CDS
CAACAATCCATCCTTCAGTT+TGG 0.407175 7.4:-84460229 MS.gene91581:CDS
GCCGAAAATTCTTCTCAATT+GGG 0.413979 7.4:-84460465 MS.gene91581:CDS
GTAGGAACAAATTTCAACTT+TGG 0.419771 7.4:-84460828 MS.gene91581:CDS
AACTTTATGATCTTCAAGAT+AGG 0.421843 7.4:-84460947 MS.gene91581:CDS
ATGATCTTCAAGATAGGTTA+GGG 0.433727 7.4:-84460941 MS.gene91581:CDS
AGCAAAGTATGCACAATAAG+AGG 0.437085 7.4:-84461014 MS.gene91581:CDS
TTTAATAAACCAATAAATGA+AGG 0.455410 7.4:+84460537 None:intergenic
CAATAGGAACAATATTCATT+GGG 0.458388 7.4:-84460760 MS.gene91581:CDS
TTGAAATTTGTTCCTACAGG+TGG 0.465228 7.4:+84460834 None:intergenic
TTGTTGAGAGAATTAGTCAT+TGG 0.470696 7.4:+84460246 None:intergenic
AAGTTGAAATTTGTTCCTAC+AGG 0.474911 7.4:+84460831 None:intergenic
TTTGATGATAATGATGGTTG+TGG 0.483862 7.4:+84460298 None:intergenic
ACTTGGTACTAATTACCAAT+GGG 0.492610 7.4:-84460505 MS.gene91581:CDS
TTCTTCAAATGTTAATGCAA+TGG 0.492790 7.4:-84460349 MS.gene91581:CDS
TAGAAATTTGGCCAAACTGA+AGG 0.496121 7.4:+84460218 None:intergenic
TTGTTGAGTTCATTAGAAGA+TGG 0.499012 7.4:+84460801 None:intergenic
GATTGAGAGATAGAAGAGTA+AGG 0.499155 7.4:-84460992 MS.gene91581:CDS
TCTTCTCAATTGGGAAACAA+TGG 0.501500 7.4:-84460456 MS.gene91581:CDS
TTATTAAATCCTATGCCACT+TGG 0.521662 7.4:-84460522 MS.gene91581:CDS
AAATGTTAATGCAATGGAGA+TGG 0.523566 7.4:-84460343 MS.gene91581:CDS
ACAGGTGGAAAATTAGGTAA+TGG 0.536982 7.4:+84460849 None:intergenic
CCAGTTGATAAAGACAACAT+TGG 0.545201 7.4:+84460414 None:intergenic
TCAACATTTGATGATAATGA+TGG 0.545561 7.4:+84460292 None:intergenic
AACCAATCAACAACTTTGCT+AGG 0.547433 7.4:+84460909 None:intergenic
TAATTAGTACCAAGTGGCAT+AGG 0.547722 7.4:+84460513 None:intergenic
GATGAAAATATTAACAAGCA+AGG 0.549563 7.4:-84460630 MS.gene91581:CDS
CACTTGGTACTAATTACCAA+TGG 0.556420 7.4:-84460506 MS.gene91581:CDS
CATTGGAAGTACCTCCCCAT+TGG 0.558728 7.4:+84460490 None:intergenic
AATTTGGCCAAACTGAAGGA+TGG 0.562622 7.4:+84460222 None:intergenic
TGGCCATTGTGATGAAGCTA+AGG 0.563573 7.4:+84461097 None:intergenic
AGGAACAATATTCATTGGGA+AGG 0.563907 7.4:-84460756 MS.gene91581:CDS
GGTGGAAAATTAGGTAATGG+TGG 0.564196 7.4:+84460852 None:intergenic
GTTGATAAAGACAACATTGG+TGG 0.570845 7.4:+84460417 None:intergenic
TCATAAAGTTGAATAGCTGT+TGG 0.592296 7.4:+84460960 None:intergenic
TTAATAAACCAATAAATGAA+GGG 0.612685 7.4:+84460538 None:intergenic
GAAGCTAAGGAGCTTGATGA+TGG 0.616225 7.4:+84461110 None:intergenic
GCTCCTTAGCTTCATCACAA+TGG 0.647279 7.4:-84461100 MS.gene91581:CDS
GGTACTAATTACCAATGGGG+AGG 0.679474 7.4:-84460501 MS.gene91581:CDS
CATTGGTAATTAGTACCAAG+TGG 0.701178 7.4:+84460507 None:intergenic
CTTGGTACTAATTACCAATG+GGG 0.737610 7.4:-84460504 MS.gene91581:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATAAACCAATAAATGAA+GGG + chr7.4:84460826-84460845 None:intergenic 15.0%
!! TTTAATAAACCAATAAATGA+AGG + chr7.4:84460827-84460846 None:intergenic 15.0%
!!! TGTAACATATTGTTATTATT+TGG + chr7.4:84460791-84460810 None:intergenic 15.0%
!! AACAATTGTTCAATTTCAAT+AGG - chr7.4:84460585-84460604 MS.gene91581:CDS 20.0%
! AAACGATCATGAAAAAGAAA+AGG - chr7.4:84460220-84460239 MS.gene91581:CDS 25.0%
! AACTTTATGATCTTCAAGAT+AGG - chr7.4:84460414-84460433 MS.gene91581:CDS 25.0%
! AATTCTCTTGAAACTTCTTT+AGG + chr7.4:84460668-84460687 None:intergenic 25.0%
! AATTCTTCTCAAATGATGTT+AGG - chr7.4:84460971-84460990 MS.gene91581:CDS 25.0%
! ATTCTCTTGAAACTTCTTTA+GGG + chr7.4:84460667-84460686 None:intergenic 25.0%
! CAATAGGAACAATATTCATT+GGG - chr7.4:84460601-84460620 MS.gene91581:CDS 25.0%
! GATGAAAATATTAACAAGCA+AGG - chr7.4:84460731-84460750 MS.gene91581:CDS 25.0%
! GTACTATTCATCAAGTAATT+TGG + chr7.4:84460704-84460723 None:intergenic 25.0%
! TAAAGAAGTTTCAAGAGAAT+TGG - chr7.4:84460667-84460686 MS.gene91581:CDS 25.0%
! TCAATAGGAACAATATTCAT+TGG - chr7.4:84460600-84460619 MS.gene91581:CDS 25.0%
! TTCTTCAAATGTTAATGCAA+TGG - chr7.4:84461012-84461031 MS.gene91581:CDS 25.0%
!! TCAACATTTGATGATAATGA+TGG + chr7.4:84461072-84461091 None:intergenic 25.0%
AAATGTTAATGCAATGGAGA+TGG - chr7.4:84461018-84461037 MS.gene91581:CDS 30.0%
ACTTGGTACTAATTACCAAT+GGG - chr7.4:84460856-84460875 MS.gene91581:CDS 30.0%
AGTGAACTCTTGTTCTTTAT+TGG + chr7.4:84461191-84461210 None:intergenic 30.0%
ATGATCTTCAAGATAGGTTA+GGG - chr7.4:84460420-84460439 MS.gene91581:CDS 30.0%
ATTCTTGGATCTTTCAATCT+TGG + chr7.4:84460287-84460306 None:intergenic 30.0%
CTTCAAGTCAAAATTCTTCT+TGG - chr7.4:84460633-84460652 MS.gene91581:CDS 30.0%
GTAGGAACAAATTTCAACTT+TGG - chr7.4:84460533-84460552 MS.gene91581:CDS 30.0%
TATGATCTTCAAGATAGGTT+AGG - chr7.4:84460419-84460438 MS.gene91581:CDS 30.0%
TCATAAAGTTGAATAGCTGT+TGG + chr7.4:84460404-84460423 None:intergenic 30.0%
TTATTAAATCCTATGCCACT+TGG - chr7.4:84460839-84460858 MS.gene91581:CDS 30.0%
TTGTTGAGAGAATTAGTCAT+TGG + chr7.4:84461118-84461137 None:intergenic 30.0%
TTGTTGAGTTCATTAGAAGA+TGG + chr7.4:84460563-84460582 None:intergenic 30.0%
TTTGGAAAAACATTTGCAGT+AGG + chr7.4:84460773-84460792 None:intergenic 30.0%
! AAGTTGAAATTTGTTCCTAC+AGG + chr7.4:84460533-84460552 None:intergenic 30.0%
! AGATTTTCCATTGAAACAAG+AGG - chr7.4:84460184-84460203 MS.gene91581:CDS 30.0%
! TCCCAATTGAGAAGAATTTT+CGG + chr7.4:84460900-84460919 None:intergenic 30.0%
! TTTGATGATAATGATGGTTG+TGG + chr7.4:84461066-84461085 None:intergenic 30.0%
!!! TCTGCTGTTTTGTAGAAATT+TGG + chr7.4:84461158-84461177 None:intergenic 30.0%
AACCAATCAACAACTTTGCT+AGG + chr7.4:84460455-84460474 None:intergenic 35.0%
AACCTAGCAAAGTTGTTGAT+TGG - chr7.4:84460450-84460469 MS.gene91581:CDS 35.0%
ACAGGTGGAAAATTAGGTAA+TGG + chr7.4:84460515-84460534 None:intergenic 35.0%
AGCAAAGTATGCACAATAAG+AGG - chr7.4:84460347-84460366 MS.gene91581:CDS 35.0%
AGGAACAATATTCATTGGGA+AGG - chr7.4:84460605-84460624 MS.gene91581:CDS 35.0%
CACTTGGTACTAATTACCAA+TGG - chr7.4:84460855-84460874 MS.gene91581:CDS 35.0%
CCAAATCACCCTTCATTTAT+TGG - chr7.4:84460815-84460834 MS.gene91581:CDS 35.0%
CCAATAAATGAAGGGTGATT+TGG + chr7.4:84460818-84460837 None:intergenic 35.0%
CCAATGTTGTCTTTATCAAC+TGG - chr7.4:84460947-84460966 MS.gene91581:CDS 35.0%
CTTGATACTCTGACAATTCT+TGG + chr7.4:84460302-84460321 None:intergenic 35.0%
CTTGGTACTAATTACCAATG+GGG - chr7.4:84460857-84460876 MS.gene91581:CDS 35.0%
GATTGAGAGATAGAAGAGTA+AGG - chr7.4:84460369-84460388 MS.gene91581:CDS 35.0%
GCCGAAAATTCTTCTCAATT+GGG - chr7.4:84460896-84460915 MS.gene91581:CDS 35.0%
TAATTAGTACCAAGTGGCAT+AGG + chr7.4:84460851-84460870 None:intergenic 35.0%
TAGAAATTTGGCCAAACTGA+AGG + chr7.4:84461146-84461165 None:intergenic 35.0%
TCTTCTCAATTGGGAAACAA+TGG - chr7.4:84460905-84460924 MS.gene91581:CDS 35.0%
TGCCGAAAATTCTTCTCAAT+TGG - chr7.4:84460895-84460914 MS.gene91581:CDS 35.0%
! AGAAGAATTTTCGGCATCAT+TGG + chr7.4:84460891-84460910 None:intergenic 35.0%
! CATTGGTAATTAGTACCAAG+TGG + chr7.4:84460857-84460876 None:intergenic 35.0%
! GTTGATAAAGACAACATTGG+TGG + chr7.4:84460947-84460966 None:intergenic 35.0%
! TGATGAACCTCTTGTTTCAA+TGG + chr7.4:84460194-84460213 None:intergenic 35.0%
! TTACCTAATTTTCCACCTGT+AGG - chr7.4:84460515-84460534 MS.gene91581:CDS 35.0%
! TTGAAATTTGTTCCTACAGG+TGG + chr7.4:84460530-84460549 None:intergenic 35.0%
!! CCAGTTGATAAAGACAACAT+TGG + chr7.4:84460950-84460969 None:intergenic 35.0%
AATTTGGCCAAACTGAAGGA+TGG + chr7.4:84461142-84461161 None:intergenic 40.0%
CAACAATCCATCCTTCAGTT+TGG - chr7.4:84461132-84461151 MS.gene91581:CDS 40.0%
GTTCCTACAGGTGGAAAATT+AGG + chr7.4:84460521-84460540 None:intergenic 40.0%
! GGTGGAAAATTAGGTAATGG+TGG + chr7.4:84460512-84460531 None:intergenic 40.0%
!! AGAGTATCAAGAGCTTTTGG+TGG - chr7.4:84460311-84460330 MS.gene91581:CDS 40.0%
!! GTCAGAGTATCAAGAGCTTT+TGG - chr7.4:84460308-84460327 MS.gene91581:CDS 40.0%
GAAGCTAAGGAGCTTGATGA+TGG + chr7.4:84460254-84460273 None:intergenic 45.0%
GCTCCTTAGCTTCATCACAA+TGG - chr7.4:84460261-84460280 MS.gene91581:CDS 45.0%
GGTACTAATTACCAATGGGG+AGG - chr7.4:84460860-84460879 MS.gene91581:CDS 45.0%
TGGCCATTGTGATGAAGCTA+AGG + chr7.4:84460267-84460286 None:intergenic 45.0%
!! CATTGGAAGTACCTCCCCAT+TGG + chr7.4:84460874-84460893 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 84460164 84461219 84460164 ID=MS.gene91581
chr7.4 mRNA 84460164 84461219 84460164 ID=MS.gene91581.t1;Parent=MS.gene91581
chr7.4 exon 84460164 84461219 84460164 ID=MS.gene91581.t1.exon1;Parent=MS.gene91581.t1
chr7.4 CDS 84460164 84461219 84460164 ID=cds.MS.gene91581.t1;Parent=MS.gene91581.t1
Gene Sequence

>MS.gene91581

ATGAATAAGAATTCAAAAGAAGATTTTCCATTGAAACAAGAGGTTCATCAAAATTCAAACGATCATGAAAAAGAAAAGGCTAATTCACCATCATCAAGCTCCTTAGCTTCATCACAATGGCCAAGATTGAAAGATCCAAGAATTGTCAGAGTATCAAGAGCTTTTGGTGGAAAAGATAGACATAGCAAAGTATGCACAATAAGAGGATTGAGAGATAGAAGAGTAAGGCTTTCAGTACCAACAGCTATTCAACTTTATGATCTTCAAGATAGGTTAGGGCTTAATCAACCTAGCAAAGTTGTTGATTGGTTGCTCAATGCAGCTAAACATGACATTGATCAACTTCCACCATTACCTAATTTTCCACCTGTAGGAACAAATTTCAACTTTGGTTTTCCATCTTCTAATGAACTCAACAATCAACAATTGTTCAATTTCAATAGGAACAATATTCATTGGGAAGGTTCAACTTCAAGTCAAAATTCTTCTTGGAAATCAAACCCTAAAGAAGTTTCAAGAGAATTGGTTAATGATAAACCAAATTACTTGATGAATAGTACTCAAGAAGATGAAAATATTAACAAGCAAGGAAGCAATATTGAAGTTCCTACTGCAAATGTTTTTCCAAATAATAACAATATGTTACAAAGACCAAATCACCCTTCATTTATTGGTTTATTAAATCCTATGCCACTTGGTACTAATTACCAATGGGGAGGTACTTCCAATGATGCCGAAAATTCTTCTCAATTGGGAAACAATGGCTTTATTACATCTTTGCCACCAATGTTGTCTTTATCAACTGGAAATTCTTCTCAAATGATGTTAGGAACAACACAATCATATTTTTCTTCAAATGTTAATGCAATGGAGATGGATCATCATCATCAAAGAGAAATCCACAACCATCATTATCATCAAATGTTGAGTTCATCAAGTTCTTCTCAGAATCCAATGACTAATTCTCTCAACAATCCATCCTTCAGTTTGGCCAAATTTCTACAAAACAGCAGAGAAAATTCATCCAATAAAGAACAAGAGTTCACTACAAAGTGA

Protein sequence

>MS.gene91581.t1

MNKNSKEDFPLKQEVHQNSNDHEKEKANSPSSSSLASSQWPRLKDPRIVRVSRAFGGKDRHSKVCTIRGLRDRRVRLSVPTAIQLYDLQDRLGLNQPSKVVDWLLNAAKHDIDQLPPLPNFPPVGTNFNFGFPSSNELNNQQLFNFNRNNIHWEGSTSSQNSSWKSNPKEVSRELVNDKPNYLMNSTQEDENINKQGSNIEVPTANVFPNNNNMLQRPNHPSFIGLLNPMPLGTNYQWGGTSNDAENSSQLGNNGFITSLPPMLSLSTGNSSQMMLGTTQSYFSSNVNAMEMDHHHQREIHNHHYHQMLSSSSSSQNPMTNSLNNPSFSLAKFLQNSRENSSNKEQEFTTK