AlfalfaGEDB | MS.gene99553

M. sativa cultivar XinJiangDaYe / MS.gene99553

KEGG: -
GO: GO:0006950 GO:0006952 GO:0008150 GO:0050896
PFAM: PF00407.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99553.t1	XP_013445246.1	95.2	62	3	0	1	62	1	62	2.60E-27	130.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99553.t1	I3SJ62	95.2	62	3	0	1	62	1	62	1.9e-27	130.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04921	MS.gene99553	0.827124	1.86E-54	-1.69E-46
MS.gene04932	MS.gene99553	0.82049	6.67E-53	-1.69E-46
MS.gene050082	MS.gene99553	0.831541	1.57E-55	-1.69E-46
MS.gene050170	MS.gene99553	-0.823175	1.60E-53	-1.69E-46
MS.gene050323	MS.gene99553	-0.807099	5.96E-50	-1.69E-46
MS.gene050338	MS.gene99553	0.803666	3.13E-49	-1.69E-46
MS.gene050496	MS.gene99553	-0.816783	4.63E-52	-1.69E-46
MS.gene050577	MS.gene99553	-0.808221	3.45E-50	-1.69E-46
MS.gene050868	MS.gene99553	-0.80037	1.49E-48	-1.69E-46
MS.gene050883	MS.gene99553	0.80689	6.60E-50	-1.69E-46
MS.gene051127	MS.gene99553	0.813026	3.15E-51	-1.69E-46
MS.gene051138	MS.gene99553	0.833797	4.34E-56	-1.69E-46
MS.gene051364	MS.gene99553	0.833684	4.63E-56	-1.69E-46
MS.gene051366	MS.gene99553	0.826277	2.96E-54	-1.69E-46
MS.gene051367	MS.gene99553	0.815499	8.96E-52	-1.69E-46
MS.gene051422	MS.gene99553	0.844752	6.22E-59	-1.69E-46
MS.gene051651	MS.gene99553	0.812268	4.62E-51	-1.69E-46
MS.gene052211	MS.gene99553	0.814744	1.32E-51	-1.69E-46
MS.gene05240	MS.gene99553	0.804408	2.19E-49	-1.69E-46
MS.gene052486	MS.gene99553	-0.801853	7.40E-49	-1.69E-46
MS.gene052494	MS.gene99553	0.803803	2.93E-49	-1.69E-46
MS.gene052606	MS.gene99553	0.84833	6.56E-60	-1.69E-46
MS.gene052702	MS.gene99553	0.802109	6.56E-49	-1.69E-46
MS.gene053097	MS.gene99553	0.815068	1.12E-51	-1.69E-46
MS.gene053420	MS.gene99553	0.841226	5.40E-58	-1.69E-46
MS.gene054706	MS.gene99553	0.812628	3.85E-51	-1.69E-46
MS.gene05470	MS.gene99553	-0.814799	1.28E-51	-1.69E-46
MS.gene055154	MS.gene99553	0.80928	2.05E-50	-1.69E-46
MS.gene055158	MS.gene99553	0.823605	1.27E-53	-1.69E-46
MS.gene055202	MS.gene99553	0.80584	1.10E-49	-1.69E-46
MS.gene055502	MS.gene99553	-0.80739	5.17E-50	-1.69E-46
MS.gene05589	MS.gene99553	-0.843099	1.73E-58	-1.69E-46
MS.gene055942	MS.gene99553	0.802007	6.88E-49	-1.69E-46
MS.gene055977	MS.gene99553	0.821264	4.43E-53	-1.69E-46
MS.gene056218	MS.gene99553	0.812523	4.06E-51	-1.69E-46
MS.gene056504	MS.gene99553	-0.835105	2.04E-56	-1.69E-46
MS.gene056590	MS.gene99553	0.819195	1.32E-52	-1.69E-46
MS.gene056591	MS.gene99553	0.820071	8.33E-53	-1.69E-46
MS.gene056694	MS.gene99553	0.848782	4.92E-60	-1.69E-46
MS.gene056697	MS.gene99553	0.82143	4.05E-53	-1.69E-46
MS.gene056748	MS.gene99553	0.821	5.10E-53	-1.69E-46
MS.gene056856	MS.gene99553	0.856134	3.95E-62	-1.69E-46
MS.gene057261	MS.gene99553	0.814567	1.44E-51	-1.69E-46
MS.gene057548	MS.gene99553	0.805228	1.48E-49	-1.69E-46
MS.gene057549	MS.gene99553	0.830348	3.09E-55	-1.69E-46
MS.gene057768	MS.gene99553	0.852177	5.48E-61	-1.69E-46
MS.gene058068	MS.gene99553	0.843113	1.71E-58	-1.69E-46
MS.gene058099	MS.gene99553	0.838535	2.72E-57	-1.69E-46
MS.gene058109	MS.gene99553	0.809835	1.56E-50	-1.69E-46
MS.gene058111	MS.gene99553	0.84699	1.53E-59	-1.69E-46
MS.gene058171	MS.gene99553	0.824588	7.44E-54	-1.69E-46
MS.gene058173	MS.gene99553	0.818034	2.42E-52	-1.69E-46
MS.gene058175	MS.gene99553	0.827331	1.66E-54	-1.69E-46
MS.gene058268	MS.gene99553	0.830835	2.35E-55	-1.69E-46
MS.gene058269	MS.gene99553	0.826654	2.41E-54	-1.69E-46
MS.gene058334	MS.gene99553	0.839562	1.47E-57	-1.69E-46
MS.gene058382	MS.gene99553	0.840336	9.25E-58	-1.69E-46
MS.gene058603	MS.gene99553	0.81569	8.12E-52	-1.69E-46
MS.gene059045	MS.gene99553	0.81193	5.47E-51	-1.69E-46
MS.gene059685	MS.gene99553	0.818798	1.62E-52	-1.69E-46
MS.gene059865	MS.gene99553	-0.82108	4.88E-53	-1.69E-46
MS.gene059953	MS.gene99553	0.80359	3.24E-49	-1.69E-46
MS.gene059955	MS.gene99553	0.822166	2.74E-53	-1.69E-46
MS.gene06000	MS.gene99553	-0.804558	2.04E-49	-1.69E-46
MS.gene060097	MS.gene99553	0.805392	1.36E-49	-1.69E-46
MS.gene060593	MS.gene99553	0.853872	1.79E-61	-1.69E-46
MS.gene060595	MS.gene99553	0.829933	3.90E-55	-1.69E-46
MS.gene060762	MS.gene99553	-0.800031	1.74E-48	-1.69E-46
MS.gene06077	MS.gene99553	-0.813291	2.76E-51	-1.69E-46
MS.gene060823	MS.gene99553	0.818365	2.04E-52	-1.69E-46
MS.gene060982	MS.gene99553	-0.821379	4.17E-53	-1.69E-46
MS.gene061044	MS.gene99553	0.854439	1.23E-61	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99553.t1	MTR_8g045695	95.161	62	3	0	1	62	1	62	3.58e-39	126
MS.gene99553.t1	MTR_8g045520	90.323	62	6	0	1	62	1	62	3.00e-36	119
MS.gene99553.t1	MTR_8g045490	87.097	62	8	0	1	62	1	62	7.62e-36	117
MS.gene99553.t1	MTR_8g045665	87.097	62	8	0	1	62	1	62	3.79e-35	116
MS.gene99553.t1	MTR_8g045735	83.871	62	10	0	1	62	1	62	7.85e-35	115
MS.gene99553.t1	MTR_8g045400	87.097	62	8	0	1	62	1	62	1.66e-34	114
MS.gene99553.t1	MTR_8g045640	83.871	62	10	0	1	62	1	62	8.07e-34	112
MS.gene99553.t1	MTR_8g045560	82.258	62	11	0	1	62	1	62	2.54e-32	108
MS.gene99553.t1	MTR_8g045300	73.770	61	16	0	1	61	1	61	7.34e-30	102
MS.gene99553.t1	MTR_8g045570	70.968	62	18	0	1	62	1	62	9.28e-29	99.8
MS.gene99553.t1	MTR_8g045555	72.581	62	17	0	1	62	1	62	2.01e-28	99.0
MS.gene99553.t1	MTR_1g030810	45.161	62	33	1	1	62	1	61	6.43e-13	59.3
MS.gene99553.t1	MTR_1g030820	37.097	62	38	1	1	62	1	61	1.69e-12	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 10 sgRNAs with CRISPR-Local

Find 9 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTAAACTTATTACTGAACT+TGG	0.415223	8.1:+59732744	MS.gene99553:CDS
AAACACTGGACTTATGTCAT+AGG	0.470664	8.1:+59732897	MS.gene99553:CDS
AGCTTCATGAGGGTGAAGAT+TGG	0.493681	8.1:+59732856	MS.gene99553:CDS
AGATTGGCATCATGCTGATT+CGG	0.494195	8.1:+59732872	MS.gene99553:CDS
ATCTTCACCCTCATGAAGCT+TGG	0.519812	8.1:-59732853	None:intergenic
GTAAACTTATTACTGAACTT+GGG	0.542207	8.1:+59732745	MS.gene99553:CDS
AGTTTGTAGAACTTGTCAGC+TGG	0.569465	8.1:-59732777	None:intergenic
ATGCTGATTCGGTTAAACAC+TGG	0.602503	8.1:+59732883	MS.gene99553:CDS
TCATCATACCAAGCTTCATG+AGG	0.610699	8.1:+59732845	MS.gene99553:CDS
CATCATACCAAGCTTCATGA+GGG	0.642119	8.1:+59732846	MS.gene99553:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GTAAACTTATTACTGAACTT+GGG	+	chr8.1:59732745-59732764	MS.gene99553:CDS	25.0%
	GGTAAACTTATTACTGAACT+TGG	+	chr8.1:59732744-59732763	MS.gene99553:CDS	30.0%
	AGTTTGTAGAACTTGTCAGC+TGG	-	chr8.1:59732780-59732799	None:intergenic	40.0%
	CATCATACCAAGCTTCATGA+GGG	+	chr8.1:59732846-59732865	MS.gene99553:CDS	40.0%
	TCATCATACCAAGCTTCATG+AGG	+	chr8.1:59732845-59732864	MS.gene99553:CDS	40.0%
!	AGATTGGCATCATGCTGATT+CGG	+	chr8.1:59732872-59732891	MS.gene99553:CDS	40.0%
!!	ATGCTGATTCGGTTAAACAC+TGG	+	chr8.1:59732883-59732902	MS.gene99553:CDS	40.0%
	AGCTTCATGAGGGTGAAGAT+TGG	+	chr8.1:59732856-59732875	MS.gene99553:CDS	45.0%
	ATCTTCACCCTCATGAAGCT+TGG	-	chr8.1:59732856-59732875	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	59732732	59732918	59732732	ID=MS.gene99553
chr8.1	mRNA	59732732	59732918	59732732	ID=MS.gene99553.t1;Parent=MS.gene99553
chr8.1	exon	59732732	59732918	59732732	ID=MS.gene99553.t1.exon1;Parent=MS.gene99553.t1
chr8.1	CDS	59732732	59732918	59732732	ID=cds.MS.gene99553.t1;Parent=MS.gene99553.t1

Gene Sequence	>MS.gene99553 ATGGTGCTTGCTGGTAAACTTATTACTGAACTTGGGATAAAAACACCAGCTGACAAGTTCTACAAACTATTTGCATCAGAACTTCACGAAGTGCAAAATGTTTGTGAAAGAGTTCATCATACCAAGCTTCATGAGGGTGAAGATTGGCATCATGCTGATTCGGTTAAACACTGGACTTATGTCATAG
Protein sequence	>MS.gene99553.t1 MVLAGKLITELGIKTPADKFYKLFASELHEVQNVCERVHHTKLHEGEDWHHADSVKHWTYVI