AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003302.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003302.01.T01 MTR_1g057860 99.333 150 1 0 4 153 719 868 2.23e-101 309
MsG0180003302.01.T01 MTR_5g091840 53.659 123 35 3 53 153 1 123 1.15e-28 102
MsG0180003302.01.T01 MTR_1g057880 95.833 48 2 0 5 52 74 121 5.47e-26 95.9
MsG0180003302.01.T01 MTR_1g022010 62.687 67 17 2 5 65 165 229 7.17e-18 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003302.01.T01 AT3G02760 58.278 151 60 2 4 151 733 883 1.23e-50 173
MsG0180003302.01.T01 AT3G02760 58.278 151 60 2 4 151 733 883 1.23e-50 173

Find 53 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGTCCTAGTGAGCATATT+AGG 0.192256 1:-60235617 MsG0180003302.01.T01:CDS
GCTACAAACACCGGGACTTA+TGG 0.237268 1:+60235338 None:intergenic
CTTCTGTTGTTGCTCCATTA+TGG 0.279186 1:+60236104 None:intergenic
CTACAAACACCGGGACTTAT+GGG 0.333119 1:+60235339 None:intergenic
TCCAGCAGTTGGTGTAAGTC+TGG 0.342849 1:-60236145 MsG0180003302.01.T01:CDS
TGGAGTTAAAGCAGAATTCT+TGG 0.351175 1:-60235546 MsG0180003302.01.T01:CDS
CATCCTACTTGGCATGTGTT+TGG 0.353353 1:-60235316 MsG0180003302.01.T01:three_prime_UTR
CGAGAGAGTATTTGCCATAA+TGG 0.385031 1:-60236118 MsG0180003302.01.T01:CDS
TTCGCATAGTCAAAGTGCTT+TGG 0.397277 1:+60235509 None:intergenic
GTTTGTCGTACTCTAGTTGA+AGG 0.399143 1:-60235221 MsG0180003302.01.T01:three_prime_UTR
GGTCGATACGATAACCTGAT+AGG 0.399560 1:-60236189 MsG0180003302.01.T01:CDS
TCGAACCAAACATCCCTATC+AGG 0.415605 1:+60236175 None:intergenic
GCTGGTGAACTGTGGGATGC+TGG 0.422884 1:-60235566 MsG0180003302.01.T01:CDS
AAGTCCTAGTGAGCATATTA+GGG 0.434249 1:-60235616 MsG0180003302.01.T01:CDS
GAAATTATCTCACGTAACTT+AGG 0.442396 1:+60235262 None:intergenic
GATAACCTGATAGGGATGTT+TGG 0.446654 1:-60236180 MsG0180003302.01.T01:CDS
GGGAATGATGTAACACTAGC+TGG 0.463967 1:-60235596 MsG0180003302.01.T01:CDS
GCTCACTAGGACTTCTGTCT+TGG 0.464076 1:+60235625 None:intergenic
GGTGTTTGTAGCATCCTACT+TGG 0.470236 1:-60235327 MsG0180003302.01.T01:three_prime_UTR
CCCAGACTTACACCAACTGC+TGG 0.472667 1:+60236144 None:intergenic
GGAGTAGACTAATTGAACTT+TGG 0.486506 1:-60235087 MsG0180003302.01.T01:three_prime_UTR
CATTCCCTAATATGCTCACT+AGG 0.506109 1:+60235612 None:intergenic
CGAAAGAATCAAGAATCCCT+TGG 0.512150 1:-60235490 MsG0180003302.01.T01:CDS
GATGATACTTGTTGGTGAGC+AGG 0.515279 1:-60235468 MsG0180003302.01.T01:CDS
CAACTGAAAAGCCTCGAGTC+AGG 0.521052 1:-60235425 MsG0180003302.01.T01:CDS
CTAAAATAACTGTTCCGTTG+TGG 0.525861 1:-60235057 MsG0180003302.01.T01:three_prime_UTR
ACTAGGACTTCTGTCTTGGT+TGG 0.529009 1:+60235629 None:intergenic
CAGGTTGGTTCAATTGCTGC+TGG 0.530641 1:-60236213 MsG0180003302.01.T01:CDS
TACTCTAGTTGAAGGAGTAT+AGG 0.534874 1:-60235213 MsG0180003302.01.T01:three_prime_UTR
ATTTATCAAATTTAGTTGAG+AGG 0.542507 1:-60235162 MsG0180003302.01.T01:three_prime_UTR
TTGTTGAGGAACTTCGGAAA+CGG 0.545775 1:-60235367 MsG0180003302.01.T01:CDS
ATCCCTTGGATGATACTTGT+TGG 0.551773 1:-60235476 MsG0180003302.01.T01:CDS
TCGAAGCAAGTTCCAGCAGT+TGG 0.553760 1:-60236156 MsG0180003302.01.T01:CDS
CCAGCAGTTGGTGTAAGTCT+GGG 0.556025 1:-60236144 MsG0180003302.01.T01:CDS
ACACTAGCTGGAGAGCTAGC+TGG 0.561864 1:-60235584 MsG0180003302.01.T01:CDS
GTCGATACGATAACCTGATA+GGG 0.570054 1:-60236188 MsG0180003302.01.T01:CDS
CATAATGGAGCAACAACAGA+AGG 0.581880 1:-60236103 MsG0180003302.01.T01:CDS
ACGGTTAAACCCATAAGTCC+CGG 0.584928 1:-60235348 MsG0180003302.01.T01:exon
ACTCCAAACACATGCCAAGT+AGG 0.591877 1:+60235313 None:intergenic
GGTGAGCAGGAAATAAAAGA+AGG 0.593607 1:-60235455 MsG0180003302.01.T01:CDS
GAGAGCTAGCTGGTGAACTG+TGG 0.602920 1:-60235574 MsG0180003302.01.T01:CDS
AAGTAGGATGCTACAAACAC+CGG 0.606252 1:+60235329 None:intergenic
AGAGCTAGCTGGTGAACTGT+GGG 0.614291 1:-60235573 MsG0180003302.01.T01:CDS
TCACCAACAAGTATCATCCA+AGG 0.624639 1:+60235473 None:intergenic
TTACATCATTACCTGACTCG+AGG 0.629762 1:+60235414 None:intergenic
GTTGGTTCAATTGCTGCTGG+TGG 0.636788 1:-60236210 MsG0180003302.01.T01:CDS
CACCAACAAGTATCATCCAA+GGG 0.637651 1:+60235474 None:intergenic
AGAGTACGACAAACAATGAA+CGG 0.639223 1:+60235230 None:intergenic
ACAACAGAAGGATCAGAACC+AGG 0.647677 1:-60236091 MsG0180003302.01.T01:intron
AGTAGGATGCTACAAACACC+GGG 0.663476 1:+60235330 None:intergenic
GCAGGAAATAAAAGAAGGCA+CGG 0.665098 1:-60235450 MsG0180003302.01.T01:CDS
TTTACACAGATAAACCACAA+CGG 0.743849 1:+60235043 None:intergenic
GTACGACAAACAATGAACGG+TGG 0.753783 1:+60235233 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGAAGAAAAAAAGAAAAT+AGG + Chr1:60235443-60235462 None:intergenic 15.0%
!!! AAAATAACTTGTGTTAAAAT+GGG + Chr1:60235261-60235280 None:intergenic 15.0%
!! AAAAACAGCAGATTATAAAA+AGG + Chr1:60235579-60235598 None:intergenic 20.0%
!! AAATATTCCTTGATTATTCT+GGG - Chr1:60235226-60235245 MsG0180003302.01.T01:three_prime_UTR 20.0%
!! ATTTATCAAATTTAGTTGAG+AGG - Chr1:60236080-60236099 MsG0180003302.01.T01:intron 20.0%
!!! AGTTGTTTCCTTATTAAATT+GGG - Chr1:60235507-60235526 MsG0180003302.01.T01:CDS 20.0%
!!! GAAAATAACTTGTGTTAAAA+TGG + Chr1:60235262-60235281 None:intergenic 20.0%
!!! TAGTTGTTTCCTTATTAAAT+TGG - Chr1:60235506-60235525 MsG0180003302.01.T01:CDS 20.0%
!!! TTTTTTTCTTCTTTTTGTTG+TGG - Chr1:60235449-60235468 MsG0180003302.01.T01:CDS 20.0%
! GAAATATTCCTTGATTATTC+TGG - Chr1:60235225-60235244 MsG0180003302.01.T01:three_prime_UTR 25.0%
! TGACACAATTATGTAATGTT+TGG + Chr1:60235289-60235308 None:intergenic 25.0%
!! AAAATTTTGTTGAGGAACTT+CGG - Chr1:60235869-60235888 MsG0180003302.01.T01:intron 25.0%
!! TCAAAAAAGATATTTTGCTG+TGG - Chr1:60236134-60236153 MsG0180003302.01.T01:CDS 25.0%
GAAATTATCTCACGTAACTT+AGG + Chr1:60235983-60236002 None:intergenic 30.0%
GTCTAGGCAATAATATAACA+AGG + Chr1:60235538-60235557 None:intergenic 30.0%
TTCAATACCCAGAATAATCA+AGG + Chr1:60235236-60235255 None:intergenic 30.0%
TTTACACAGATAAACCACAA+CGG + Chr1:60236202-60236221 None:intergenic 30.0%
! CCTCAACAAAATTTTCTCTA+GGG + Chr1:60235864-60235883 None:intergenic 30.0%
! TCCTCAACAAAATTTTCTCT+AGG + Chr1:60235865-60235884 None:intergenic 30.0%
!! TTCTTTTTGTTGTGGTGTTT+TGG - Chr1:60235457-60235476 MsG0180003302.01.T01:CDS 30.0%
!!! AATCTGCTGTTTTTGTTAGA+TGG - Chr1:60235585-60235604 MsG0180003302.01.T01:CDS 30.0%
AACTGTTAGTTCATCTGTCT+AGG + Chr1:60235554-60235573 None:intergenic 35.0%
AAGTCCTAGTGAGCATATTA+GGG - Chr1:60235626-60235645 MsG0180003302.01.T01:CDS 35.0%
AGAGTACGACAAACAATGAA+CGG + Chr1:60236015-60236034 None:intergenic 35.0%
AGGACGTTCCCAATTTAATA+AGG + Chr1:60235518-60235537 None:intergenic 35.0%
CTAAAATAACTGTTCCGTTG+TGG - Chr1:60236185-60236204 MsG0180003302.01.T01:CDS 35.0%
GGAGTAGACTAATTGAACTT+TGG - Chr1:60236155-60236174 MsG0180003302.01.T01:CDS 35.0%
TATCAAGCATTATGATCACC+TGG + Chr1:60235172-60235191 None:intergenic 35.0%
TATCTATCTGATGGAGGATA+TGG + Chr1:60235328-60235347 None:intergenic 35.0%
TGGAGTTAAAGCAGAATTCT+TGG - Chr1:60235696-60235715 MsG0180003302.01.T01:intron 35.0%
! CCCTAGAGAAAATTTTGTTG+AGG - Chr1:60235861-60235880 MsG0180003302.01.T01:intron 35.0%
! TACTCTAGTTGAAGGAGTAT+AGG - Chr1:60236029-60236048 MsG0180003302.01.T01:intron 35.0%
!! CACAACGGAACAGTTATTTT+AGG + Chr1:60236187-60236206 None:intergenic 35.0%
!! GCGTTTTCACAATGATAATG+CGG - Chr1:60235954-60235973 MsG0180003302.01.T01:intron 35.0%
!!! ACGGAACAGTTATTTTAGGT+TGG + Chr1:60236183-60236202 None:intergenic 35.0%
AAGTAGGATGCTACAAACAC+CGG + Chr1:60235916-60235935 None:intergenic 40.0%
ATCCCTTGGATGATACTTGT+TGG - Chr1:60235766-60235785 MsG0180003302.01.T01:intron 40.0%
ATGACTTGATTCATCACCAC+GGG - Chr1:60235377-60235396 MsG0180003302.01.T01:CDS 40.0%
CACCAACAAGTATCATCCAA+GGG + Chr1:60235771-60235790 None:intergenic 40.0%
CAGACAGTATAAGTAACAGC+AGG + Chr1:60235353-60235372 None:intergenic 40.0%
CATTCCCTAATATGCTCACT+AGG + Chr1:60235633-60235652 None:intergenic 40.0%
CGAAAGAATCAAGAATCCCT+TGG - Chr1:60235752-60235771 MsG0180003302.01.T01:intron 40.0%
CTTCTGTTGTTGCTCCATTA+TGG + Chr1:60235141-60235160 None:intergenic 40.0%
GAAGTCCTAGTGAGCATATT+AGG - Chr1:60235625-60235644 MsG0180003302.01.T01:CDS 40.0%
GATAACCTGATAGGGATGTT+TGG - Chr1:60235062-60235081 MsG0180003302.01.T01:three_prime_UTR 40.0%
GATGACTTGATTCATCACCA+CGG - Chr1:60235376-60235395 MsG0180003302.01.T01:CDS 40.0%
GCAGGAAATAAAAGAAGGCA+CGG - Chr1:60235792-60235811 MsG0180003302.01.T01:intron 40.0%
GGGATGTATCTAATCACTAG+GGG - Chr1:60235398-60235417 MsG0180003302.01.T01:CDS 40.0%
GGGGATGTATCTAATCACTA+GGG - Chr1:60235397-60235416 MsG0180003302.01.T01:CDS 40.0%
GGTGAGCAGGAAATAAAAGA+AGG - Chr1:60235787-60235806 MsG0180003302.01.T01:intron 40.0%
GTCGATACGATAACCTGATA+GGG - Chr1:60235054-60235073 MsG0180003302.01.T01:three_prime_UTR 40.0%
GTTTGTCGTACTCTAGTTGA+AGG - Chr1:60236021-60236040 MsG0180003302.01.T01:intron 40.0%
TCACCAACAAGTATCATCCA+AGG + Chr1:60235772-60235791 None:intergenic 40.0%
TCTGATGGAGGATATGGTTT+AGG + Chr1:60235322-60235341 None:intergenic 40.0%
TTGTTGAGGAACTTCGGAAA+CGG - Chr1:60235875-60235894 MsG0180003302.01.T01:intron 40.0%
! CATAATGGAGCAACAACAGA+AGG - Chr1:60235139-60235158 MsG0180003302.01.T01:three_prime_UTR 40.0%
! CTGCTGTTACTTATACTGTC+TGG - Chr1:60235351-60235370 MsG0180003302.01.T01:exon 40.0%
! TTACATCATTACCTGACTCG+AGG + Chr1:60235831-60235850 None:intergenic 40.0%
! TTCGCATAGTCAAAGTGCTT+TGG + Chr1:60235736-60235755 None:intergenic 40.0%
!! CGAGAGAGTATTTGCCATAA+TGG - Chr1:60235124-60235143 MsG0180003302.01.T01:three_prime_UTR 40.0%
ACAACAGAAGGATCAGAACC+AGG - Chr1:60235151-60235170 MsG0180003302.01.T01:three_prime_UTR 45.0%
ACGGTTAAACCCATAAGTCC+CGG - Chr1:60235894-60235913 MsG0180003302.01.T01:intron 45.0%
ACTAGGACTTCTGTCTTGGT+TGG + Chr1:60235616-60235635 None:intergenic 45.0%
ACTCCAAACACATGCCAAGT+AGG + Chr1:60235932-60235951 None:intergenic 45.0%
AGTAGGATGCTACAAACACC+GGG + Chr1:60235915-60235934 None:intergenic 45.0%
AGTGATTAGATACATCCCCG+TGG + Chr1:60235396-60235415 None:intergenic 45.0%
CATCCTACTTGGCATGTGTT+TGG - Chr1:60235926-60235945 MsG0180003302.01.T01:intron 45.0%
CGGGGATGTATCTAATCACT+AGG - Chr1:60235396-60235415 MsG0180003302.01.T01:CDS 45.0%
CTACAAACACCGGGACTTAT+GGG + Chr1:60235906-60235925 None:intergenic 45.0%
GGGAATGATGTAACACTAGC+TGG - Chr1:60235646-60235665 MsG0180003302.01.T01:intron 45.0%
GGTCGATACGATAACCTGAT+AGG - Chr1:60235053-60235072 MsG0180003302.01.T01:three_prime_UTR 45.0%
GGTGTTTGTAGCATCCTACT+TGG - Chr1:60235915-60235934 MsG0180003302.01.T01:intron 45.0%
GTACGACAAACAATGAACGG+TGG + Chr1:60236012-60236031 None:intergenic 45.0%
TCGAACCAAACATCCCTATC+AGG + Chr1:60235070-60235089 None:intergenic 45.0%
TGACTTGATTCATCACCACG+GGG - Chr1:60235378-60235397 MsG0180003302.01.T01:CDS 45.0%
! GATGATACTTGTTGGTGAGC+AGG - Chr1:60235774-60235793 MsG0180003302.01.T01:intron 45.0%
!! CAGCAGGAGTATCTATCTGA+TGG + Chr1:60235337-60235356 None:intergenic 45.0%
!! CAGGAGTATCTATCTGATGG+AGG + Chr1:60235334-60235353 None:intergenic 45.0%
AGAGCTAGCTGGTGAACTGT+GGG - Chr1:60235669-60235688 MsG0180003302.01.T01:intron 50.0%
CAACTGAAAAGCCTCGAGTC+AGG - Chr1:60235817-60235836 MsG0180003302.01.T01:intron 50.0%
GCTACAAACACCGGGACTTA+TGG + Chr1:60235907-60235926 None:intergenic 50.0%
GCTCACTAGGACTTCTGTCT+TGG + Chr1:60235620-60235639 None:intergenic 50.0%
TCGAAGCAAGTTCCAGCAGT+TGG - Chr1:60235086-60235105 MsG0180003302.01.T01:three_prime_UTR 50.0%
! CCAGCAGTTGGTGTAAGTCT+GGG - Chr1:60235098-60235117 MsG0180003302.01.T01:three_prime_UTR 50.0%
! GTTGGTTCAATTGCTGCTGG+TGG - Chr1:60235032-60235051 MsG0180003302.01.T01:three_prime_UTR 50.0%
! TCCAGCAGTTGGTGTAAGTC+TGG - Chr1:60235097-60235116 MsG0180003302.01.T01:three_prime_UTR 50.0%
!! CAGGTTGGTTCAATTGCTGC+TGG - Chr1:60235029-60235048 MsG0180003302.01.T01:three_prime_UTR 50.0%
ACACTAGCTGGAGAGCTAGC+TGG - Chr1:60235658-60235677 MsG0180003302.01.T01:intron 55.0%
CCCAGACTTACACCAACTGC+TGG + Chr1:60235101-60235120 None:intergenic 55.0%
GAGAGCTAGCTGGTGAACTG+TGG - Chr1:60235668-60235687 MsG0180003302.01.T01:intron 55.0%
GCTGGTGAACTGTGGGATGC+TGG - Chr1:60235676-60235695 MsG0180003302.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 60235017 60236247 60235017 ID=MsG0180003302.01;Name=MsG0180003302.01
Chr1 mRNA 60235017 60236247 60235017 ID=MsG0180003302.01.T01;Parent=MsG0180003302.01;Name=MsG0180003302.01.T01;_AED=0.48;_eAED=0.48;_QI=0|1|0.5|1|1|1|2|338|153
Chr1 exon 60235017 60235660 60235017 ID=MsG0180003302.01.T01:exon:4033;Parent=MsG0180003302.01.T01
Chr1 exon 60236092 60236247 60236092 ID=MsG0180003302.01.T01:exon:4032;Parent=MsG0180003302.01.T01
Chr1 CDS 60236092 60236247 60236092 ID=MsG0180003302.01.T01:cds;Parent=MsG0180003302.01.T01
Chr1 CDS 60235355 60235660 60235355 ID=MsG0180003302.01.T01:cds;Parent=MsG0180003302.01.T01
Chr1 three_prime_UTR 60235017 60235354 60235017 ID=MsG0180003302.01.T01:three_prime_utr;Parent=MsG0180003302.01.T01
Gene Sequence

>MsG0180003302.01.T01

ATGGTTTTTACACAGGTTGGTTCAATTGCTGCTGGTGGTCGATACGATAACCTGATAGGGATGTTTGGTTCGAAGCAAGTTCCAGCAGTTGGTGTAAGTCTGGGAATCGAGAGAGTATTTGCCATAATGGAGCAACAACAGAAGGATCAGAACCAGATGGCTCGACCAACCAAGACAGAAGTCCTAGTGAGCATATTAGGGAATGATGTAACACTAGCTGGAGAGCTAGCTGGTGAACTGTGGGATGCTGGAGTTAAAGCAGAATTCTTGGTCAACAAAAGAAGACCAAAGCACTTTGACTATGCGAAAGAATCAAGAATCCCTTGGATGATACTTGTTGGTGAGCAGGAAATAAAAGAAGGCACGGTGCAACTGAAAAGCCTCGAGTCAGGTAATGATGTAAACATAAACATCCCTAGAGAAAATTTTGTTGAGGAACTTCGGAAACGGTTAAACCCATAA

Protein sequence

>MsG0180003302.01.T01

MVFTQVGSIAAGGRYDNLIGMFGSKQVPAVGVSLGIERVFAIMEQQQKDQNQMARPTKTEVLVSILGNDVTLAGELAGELWDAGVKAEFLVNKRRPKHFDYAKESRIPWMILVGEQEIKEGTVQLKSLESGNDVNINIPRENFVEELRKRLNP*