AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280009234.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009234.01.T01 MTR_2g055990 95.785 261 8 2 1 260 1 259 0.0 516
MsG0280009234.01.T01 MTR_4g112370 75.000 256 49 5 8 260 17 260 8.11e-130 368
MsG0280009234.01.T01 MTR_2g054380 68.852 183 55 2 78 260 90 270 1.34e-84 254
MsG0280009234.01.T01 MTR_1g053130 58.382 173 67 2 93 260 112 284 5.21e-59 189
MsG0280009234.01.T01 MTR_3g094830 44.643 168 88 4 94 260 96 259 1.35e-47 159
MsG0280009234.01.T01 MTR_8g040690 43.787 169 87 4 94 260 85 247 2.34e-46 155
MsG0280009234.01.T01 MTR_8g040680 43.195 169 88 4 94 260 95 257 2.24e-44 150
MsG0280009234.01.T01 MTR_3g101300 45.000 180 89 5 86 259 60 235 3.23e-44 149
MsG0280009234.01.T01 MTR_7g064810 45.732 164 82 4 97 259 65 222 5.69e-39 135
MsG0280009234.01.T01 MTR_3g086740 36.723 177 103 5 87 259 71 242 6.87e-32 117
MsG0280009234.01.T01 MTR_8g089865 37.576 165 96 5 99 259 59 220 1.07e-29 111
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009234.01.T01 AT5G23100 55.282 284 92 6 4 260 2 277 4.73e-93 275
MsG0280009234.01.T01 AT3G25640 67.797 177 47 3 92 260 93 267 3.25e-79 240
MsG0280009234.01.T01 AT3G25640 67.797 177 47 3 92 260 119 293 3.34e-79 241
MsG0280009234.01.T01 AT2G41660 53.409 176 71 4 93 260 125 297 3.88e-59 190
MsG0280009234.01.T01 AT4G39610 44.186 172 88 2 95 260 95 264 3.21e-51 168
MsG0280009234.01.T01 AT5G06990 47.727 176 79 4 94 260 90 261 7.17e-50 165
MsG0280009234.01.T01 AT2G21990 45.509 167 87 2 94 260 90 252 1.26e-49 164
MsG0280009234.01.T01 AT2G37880 46.988 166 83 3 97 259 81 244 3.57e-45 152
MsG0280009234.01.T01 AT5G42680 47.239 163 79 4 99 259 79 236 3.90e-43 147
MsG0280009234.01.T01 AT5G42680 47.239 163 79 4 99 259 79 236 3.90e-43 147
MsG0280009234.01.T01 AT1G21050 42.945 163 84 3 99 259 89 244 3.26e-39 137
MsG0280009234.01.T01 AT1G21050 42.945 163 84 3 99 259 125 280 6.66e-39 137
MsG0280009234.01.T01 AT5G65340 42.012 169 86 4 99 259 85 249 4.13e-34 124
MsG0280009234.01.T01 AT1G76610 44.048 168 77 4 94 259 73 225 4.41e-34 123
MsG0280009234.01.T01 AT2G22460 40.491 163 90 3 97 258 85 241 4.79e-30 113

Find 65 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGTTCGCGAAATGGCATC+TGG 0.291635 2:-48447543 MsG0280009234.01.T01:CDS
CAGTGGTTTGGTTCGCGAAA+TGG 0.341965 2:-48447550 MsG0280009234.01.T01:CDS
GGCCTTGGTTCCCGTGTAGT+TGG 0.378975 2:-48447669 MsG0280009234.01.T01:CDS
GTTATACCAGGGACTGATAA+TGG 0.382078 2:-48447330 MsG0280009234.01.T01:CDS
TCGAACACACCTTTCGGCCT+TGG 0.390231 2:-48447684 MsG0280009234.01.T01:CDS
TGTTGCAAGCTCAATCAAAA+AGG 0.391243 2:+48447578 None:intergenic
TCAGGAACACCATTGCTATC+AGG 0.394368 2:+48447210 None:intergenic
GCAAAGTTTGAGAGAATTGT+TGG 0.396020 2:-48447267 MsG0280009234.01.T01:CDS
CCTCCTTGTTGCATGTGCAC+TGG 0.403489 2:+48447770 None:intergenic
CGCTCAGCTCTCACCGTGTT+CGG 0.408847 2:-48447717 MsG0280009234.01.T01:CDS
CGTGTAGTTGGAACACTCTT+CGG 0.416184 2:-48447657 MsG0280009234.01.T01:CDS
AACCGGTCGAACACACCTTT+CGG 0.418706 2:-48447690 MsG0280009234.01.T01:CDS
TGCAACACCGATCAGTGGTT+TGG 0.422361 2:-48447562 MsG0280009234.01.T01:CDS
TGAGAGCAGTGGCTATTTCA+AGG 0.439057 2:-48447743 MsG0280009234.01.T01:CDS
GGCCGAAAGGTGTGTTCGAC+CGG 0.450421 2:+48447688 None:intergenic
CCGAAGAGAATGCGGGCAAA+AGG 0.452366 2:-48447400 MsG0280009234.01.T01:CDS
ACACCAGCACCCATTGAAAT+CGG 0.454573 2:+48447351 None:intergenic
TGGGTCAGTAAGAATTGCTC+TGG 0.468506 2:-48447523 MsG0280009234.01.T01:CDS
TCACCGTGTTCGGCAAGAAC+CGG 0.473449 2:-48447707 MsG0280009234.01.T01:CDS
AGCAAATAAATACTAAGCTC+AGG 0.482261 2:+48447192 None:intergenic
AGCTGTGGAGCCGATTTCAA+TGG 0.488272 2:-48447361 MsG0280009234.01.T01:CDS
CAAAGTTTGAGAGAATTGTT+GGG 0.491568 2:-48447266 MsG0280009234.01.T01:CDS
ATGGGTGCTGGTGTTATACC+AGG 0.505030 2:-48447342 MsG0280009234.01.T01:CDS
AACAAGGTTGGAACTGAAGA+TGG 0.506695 2:-48447894 MsG0280009234.01.T01:CDS
GAGAATGCGGGCAAAAGGAG+TGG 0.513741 2:-48447395 MsG0280009234.01.T01:CDS
TTTCATTGGACAAACAAGGT+TGG 0.516802 2:-48447906 MsG0280009234.01.T01:CDS
TCTGCTCCATTATCAGTCCC+TGG 0.519108 2:+48447324 None:intergenic
AAGCAGAGAAGAAAAGCTTA+AGG 0.521495 2:-48447482 MsG0280009234.01.T01:CDS
ACTGATAATGGAGCAGAAGC+AGG 0.532733 2:-48447318 MsG0280009234.01.T01:CDS
GCTGTGGAGCCGATTTCAAT+GGG 0.538795 2:-48447360 MsG0280009234.01.T01:CDS
TGGTTCGCGAAATGGCATCT+GGG 0.541090 2:-48447542 MsG0280009234.01.T01:CDS
CGACCGGTTCTTGCCGAACA+CGG 0.544927 2:+48447704 None:intergenic
TGGAGCAGAAGCAGGACAAG+AGG 0.545912 2:-48447310 MsG0280009234.01.T01:CDS
GAGCCGATTTCAATGGGTGC+TGG 0.554131 2:-48447354 MsG0280009234.01.T01:CDS
CGAAGAGTGTTCCAACTACA+CGG 0.554132 2:+48447658 None:intergenic
GGATAAGAAAGAGGCTGACA+AGG 0.563529 2:-48447826 MsG0280009234.01.T01:CDS
TGACAAGGTTGTTGATCCTG+AGG 0.564550 2:-48447811 MsG0280009234.01.T01:CDS
TATTGCAATGGCAAGAAATG+TGG 0.567073 2:-48447432 MsG0280009234.01.T01:CDS
TCAGAAGGAGGATAAGAAAG+AGG 0.581589 2:-48447835 MsG0280009234.01.T01:CDS
GGAGCAGAAGCAGGACAAGA+GGG 0.589548 2:-48447309 MsG0280009234.01.T01:CDS
TTGCTAACCGAAGAGAATGC+GGG 0.590108 2:-48447407 MsG0280009234.01.T01:CDS
GTAGCAACTGATGAAACCTC+AGG 0.592839 2:+48447795 None:intergenic
TTCGACGCTCATTCAGAAGG+AGG 0.595692 2:-48447847 MsG0280009234.01.T01:CDS
ACACTCTTCGGATACAGACG+TGG 0.614842 2:-48447645 MsG0280009234.01.T01:CDS
AGGGTGAACTTATGTACATG+AGG 0.615478 2:-48447290 MsG0280009234.01.T01:CDS
GAGCTTGCAACACCGATCAG+TGG 0.615629 2:-48447567 MsG0280009234.01.T01:CDS
CAGCCAGTGCACATGCAACA+AGG 0.617424 2:-48447773 MsG0280009234.01.T01:CDS
AAGGAGGAAGCTGAGAGCAG+TGG 0.617498 2:-48447754 MsG0280009234.01.T01:CDS
AGAATGCGGGCAAAAGGAGT+GGG 0.620572 2:-48447394 MsG0280009234.01.T01:CDS
GTGGGATATTCTCAAAGCTG+TGG 0.621077 2:-48447376 MsG0280009234.01.T01:CDS
TTTGCTAACCGAAGAGAATG+CGG 0.625223 2:-48447408 MsG0280009234.01.T01:CDS
TTCCAACTACACGGGAACCA+AGG 0.626208 2:+48447667 None:intergenic
ACACGGGAACCAAGGCCGAA+AGG 0.631113 2:+48447675 None:intergenic
TCGCGAACCAAACCACTGAT+CGG 0.634720 2:+48447555 None:intergenic
GGTTGCTTGAAGAGCCTCAG+TGG 0.639061 2:-48447461 MsG0280009234.01.T01:CDS
TGCTTGAAGAGCCTCAGTGG+AGG 0.649149 2:-48447458 MsG0280009234.01.T01:CDS
CAGTGGAGGACATATTGCAA+TGG 0.652311 2:-48447444 MsG0280009234.01.T01:CDS
AATTGCTCTGGAATGTGACA+AGG 0.655325 2:-48447511 MsG0280009234.01.T01:CDS
CACTCTTCGGATACAGACGT+GGG 0.655753 2:-48447644 MsG0280009234.01.T01:CDS
GGGTGAACTTATGTACATGA+GGG 0.663670 2:-48447289 MsG0280009234.01.T01:CDS
GAAGAGTGTTCCAACTACAC+GGG 0.678484 2:+48447659 None:intergenic
TGGGTGCTGGTGTTATACCA+GGG 0.691210 2:-48447341 MsG0280009234.01.T01:CDS
TTGCAATATGTCCTCCACTG+AGG 0.726059 2:+48447447 None:intergenic
CCAGTGCACATGCAACAAGG+AGG 0.734108 2:-48447770 MsG0280009234.01.T01:CDS
ATGATGAATCCTGATAGCAA+TGG 0.740095 2:-48447219 MsG0280009234.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTACATTTTGCATTTCAAA+AGG - Chr2:48447505-48447524 MsG0280009234.01.T01:CDS 25.0%
AGCAAATAAATACTAAGCTC+AGG + Chr2:48447935-48447954 None:intergenic 30.0%
CAAAGTTTGAGAGAATTGTT+GGG - Chr2:48447858-48447877 MsG0280009234.01.T01:CDS 30.0%
! ACATTTTCATTGGACAAACA+AGG - Chr2:48447214-48447233 MsG0280009234.01.T01:CDS 30.0%
!! CTTCTAAGAGACATTTTCAT+TGG - Chr2:48447204-48447223 MsG0280009234.01.T01:CDS 30.0%
AAGCAGAGAAGAAAAGCTTA+AGG - Chr2:48447642-48447661 MsG0280009234.01.T01:CDS 35.0%
ATGATGAATCCTGATAGCAA+TGG - Chr2:48447905-48447924 MsG0280009234.01.T01:CDS 35.0%
CTCAATCAAAAAGGCTTCTT+GGG + Chr2:48447540-48447559 None:intergenic 35.0%
GCAAAGTTTGAGAGAATTGT+TGG - Chr2:48447857-48447876 MsG0280009234.01.T01:CDS 35.0%
TATTGCAATGGCAAGAAATG+TGG - Chr2:48447692-48447711 MsG0280009234.01.T01:CDS 35.0%
TGTTGCAAGCTCAATCAAAA+AGG + Chr2:48447549-48447568 None:intergenic 35.0%
TTTCATTGGACAAACAAGGT+TGG - Chr2:48447218-48447237 MsG0280009234.01.T01:CDS 35.0%
AAAAAGGCTTCTTGGGAAGT+TGG + Chr2:48447533-48447552 None:intergenic 40.0%
AACAAGGTTGGAACTGAAGA+TGG - Chr2:48447230-48447249 MsG0280009234.01.T01:CDS 40.0%
AGGGTGAACTTATGTACATG+AGG - Chr2:48447834-48447853 MsG0280009234.01.T01:CDS 40.0%
GCTCAATCAAAAAGGCTTCT+TGG + Chr2:48447541-48447560 None:intergenic 40.0%
GGGTGAACTTATGTACATGA+GGG - Chr2:48447835-48447854 MsG0280009234.01.T01:CDS 40.0%
GTTATACCAGGGACTGATAA+TGG - Chr2:48447794-48447813 MsG0280009234.01.T01:CDS 40.0%
TCAGAAGGAGGATAAGAAAG+AGG - Chr2:48447289-48447308 MsG0280009234.01.T01:CDS 40.0%
TTTGCTAACCGAAGAGAATG+CGG - Chr2:48447716-48447735 MsG0280009234.01.T01:CDS 40.0%
!! AATTGCTCTGGAATGTGACA+AGG - Chr2:48447613-48447632 MsG0280009234.01.T01:CDS 40.0%
ACACCAGCACCCATTGAAAT+CGG + Chr2:48447776-48447795 None:intergenic 45.0%
CAGTGGAGGACATATTGCAA+TGG - Chr2:48447680-48447699 MsG0280009234.01.T01:CDS 45.0%
CGTGTAGTTGGAACACTCTT+CGG - Chr2:48447467-48447486 MsG0280009234.01.T01:CDS 45.0%
GGATAAGAAAGAGGCTGACA+AGG - Chr2:48447298-48447317 MsG0280009234.01.T01:CDS 45.0%
GTAGCAACTGATGAAACCTC+AGG + Chr2:48447332-48447351 None:intergenic 45.0%
GTGGGATATTCTCAAAGCTG+TGG - Chr2:48447748-48447767 MsG0280009234.01.T01:CDS 45.0%
TCAGGAACACCATTGCTATC+AGG + Chr2:48447917-48447936 None:intergenic 45.0%
TGACAAGGTTGTTGATCCTG+AGG - Chr2:48447313-48447332 MsG0280009234.01.T01:CDS 45.0%
TGGGTCAGTAAGAATTGCTC+TGG - Chr2:48447601-48447620 MsG0280009234.01.T01:CDS 45.0%
TTGCAATATGTCCTCCACTG+AGG + Chr2:48447680-48447699 None:intergenic 45.0%
TTGCTAACCGAAGAGAATGC+GGG - Chr2:48447717-48447736 MsG0280009234.01.T01:CDS 45.0%
! GTTTTCGACGCTCATTCAGA+AGG - Chr2:48447274-48447293 MsG0280009234.01.T01:CDS 45.0%
! TGAGAGCAGTGGCTATTTCA+AGG - Chr2:48447381-48447400 MsG0280009234.01.T01:CDS 45.0%
!! ACTGATAATGGAGCAGAAGC+AGG - Chr2:48447806-48447825 MsG0280009234.01.T01:CDS 45.0%
!! CGAAGAGTGTTCCAACTACA+CGG + Chr2:48447469-48447488 None:intergenic 45.0%
!! GAAGAGTGTTCCAACTACAC+GGG + Chr2:48447468-48447487 None:intergenic 45.0%
AACCGGTCGAACACACCTTT+CGG - Chr2:48447434-48447453 MsG0280009234.01.T01:CDS 50.0%
ACACTCTTCGGATACAGACG+TGG - Chr2:48447479-48447498 MsG0280009234.01.T01:CDS 50.0%
AGAATGCGGGCAAAAGGAGT+GGG - Chr2:48447730-48447749 MsG0280009234.01.T01:CDS 50.0%
CACTCTTCGGATACAGACGT+GGG - Chr2:48447480-48447499 MsG0280009234.01.T01:CDS 50.0%
TCGCGAACCAAACCACTGAT+CGG + Chr2:48447572-48447591 None:intergenic 50.0%
TCTGCTCCATTATCAGTCCC+TGG + Chr2:48447803-48447822 None:intergenic 50.0%
TGCAACACCGATCAGTGGTT+TGG - Chr2:48447562-48447581 MsG0280009234.01.T01:CDS 50.0%
TGGTTCGCGAAATGGCATCT+GGG - Chr2:48447582-48447601 MsG0280009234.01.T01:CDS 50.0%
TTCCAACTACACGGGAACCA+AGG + Chr2:48447460-48447479 None:intergenic 50.0%
TTCGACGCTCATTCAGAAGG+AGG - Chr2:48447277-48447296 MsG0280009234.01.T01:CDS 50.0%
! AGCTGTGGAGCCGATTTCAA+TGG - Chr2:48447763-48447782 MsG0280009234.01.T01:CDS 50.0%
! CCTTTTGCCCGCATTCTCTT+CGG + Chr2:48447727-48447746 None:intergenic 50.0%
! GCTGTGGAGCCGATTTCAAT+GGG - Chr2:48447764-48447783 MsG0280009234.01.T01:CDS 50.0%
! TTGGTTCGCGAAATGGCATC+TGG - Chr2:48447581-48447600 MsG0280009234.01.T01:CDS 50.0%
!! ATGGGTGCTGGTGTTATACC+AGG - Chr2:48447782-48447801 MsG0280009234.01.T01:CDS 50.0%
!! CAGTGGTTTGGTTCGCGAAA+TGG - Chr2:48447574-48447593 MsG0280009234.01.T01:CDS 50.0%
!! TGGGTGCTGGTGTTATACCA+GGG - Chr2:48447783-48447802 MsG0280009234.01.T01:CDS 50.0%
AAGGAGGAAGCTGAGAGCAG+TGG - Chr2:48447370-48447389 MsG0280009234.01.T01:CDS 55.0%
CAGCCAGTGCACATGCAACA+AGG - Chr2:48447351-48447370 MsG0280009234.01.T01:CDS 55.0%
CCAGTGCACATGCAACAAGG+AGG - Chr2:48447354-48447373 MsG0280009234.01.T01:CDS 55.0%
CCGAAGAGAATGCGGGCAAA+AGG - Chr2:48447724-48447743 MsG0280009234.01.T01:CDS 55.0%
CCTCCTTGTTGCATGTGCAC+TGG + Chr2:48447357-48447376 None:intergenic 55.0%
GAGAATGCGGGCAAAAGGAG+TGG - Chr2:48447729-48447748 MsG0280009234.01.T01:CDS 55.0%
GAGCTTGCAACACCGATCAG+TGG - Chr2:48447557-48447576 MsG0280009234.01.T01:CDS 55.0%
GGAGCAGAAGCAGGACAAGA+GGG - Chr2:48447815-48447834 MsG0280009234.01.T01:CDS 55.0%
GGTTGCTTGAAGAGCCTCAG+TGG - Chr2:48447663-48447682 MsG0280009234.01.T01:CDS 55.0%
TCACCGTGTTCGGCAAGAAC+CGG - Chr2:48447417-48447436 MsG0280009234.01.T01:CDS 55.0%
TCGAACACACCTTTCGGCCT+TGG - Chr2:48447440-48447459 MsG0280009234.01.T01:CDS 55.0%
TGCTTGAAGAGCCTCAGTGG+AGG - Chr2:48447666-48447685 MsG0280009234.01.T01:CDS 55.0%
TGGAGCAGAAGCAGGACAAG+AGG - Chr2:48447814-48447833 MsG0280009234.01.T01:CDS 55.0%
! GAGCCGATTTCAATGGGTGC+TGG - Chr2:48447770-48447789 MsG0280009234.01.T01:CDS 55.0%
ACACGGGAACCAAGGCCGAA+AGG + Chr2:48447452-48447471 None:intergenic 60.0%
CGCTCAGCTCTCACCGTGTT+CGG - Chr2:48447407-48447426 MsG0280009234.01.T01:CDS 60.0%
GGCCTTGGTTCCCGTGTAGT+TGG - Chr2:48447455-48447474 MsG0280009234.01.T01:CDS 60.0%
! CGACCGGTTCTTGCCGAACA+CGG + Chr2:48447423-48447442 None:intergenic 60.0%
! GGCCGAAAGGTGTGTTCGAC+CGG + Chr2:48447439-48447458 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 48447182 48447964 48447182 ID=MsG0280009234.01;Name=MsG0280009234.01
Chr2 mRNA 48447182 48447964 48447182 ID=MsG0280009234.01.T01;Parent=MsG0280009234.01;Name=MsG0280009234.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|260
Chr2 exon 48447182 48447964 48447182 ID=MsG0280009234.01.T01:exon:44814;Parent=MsG0280009234.01.T01
Chr2 CDS 48447182 48447964 48447182 ID=MsG0280009234.01.T01:cds;Parent=MsG0280009234.01.T01
Gene Sequence

>MsG0280009234.01.T01

ATGGCTAGAAGCTCAAACATGTCTTCTAAGAGACATTTTCATTGGACAAACAAGGTTGGAACTGAAGATGGTGAAGTTCACACTTCAGAATCGTTTTCGACGCTCATTCAGAAGGAGGATAAGAAAGAGGCTGACAAGGTTGTTGATCCTGAGGTTTCATCAGTTGCTACAGCCAGTGCACATGCAACAAGGAGGAAGCTGAGAGCAGTGGCTATTTCAAGGCTTCGCTCAGCTCTCACCGTGTTCGGCAAGAACCGGTCGAACACACCTTTCGGCCTTGGTTCCCGTGTAGTTGGAACACTCTTCGGATACAGACGTGGGCATGTACATTTTGCATTTCAAAAGGATCCAACTTCCCAAGAAGCCTTTTTGATTGAGCTTGCAACACCGATCAGTGGTTTGGTTCGCGAAATGGCATCTGGGTCAGTAAGAATTGCTCTGGAATGTGACAAGGAGAAAGAAGCAGAGAAGAAAAGCTTAAGGTTGCTTGAAGAGCCTCAGTGGAGGACATATTGCAATGGCAAGAAATGTGGATTTGCTAACCGAAGAGAATGCGGGCAAAAGGAGTGGGATATTCTCAAAGCTGTGGAGCCGATTTCAATGGGTGCTGGTGTTATACCAGGGACTGATAATGGAGCAGAAGCAGGACAAGAGGGTGAACTTATGTACATGAGGGCAAAGTTTGAGAGAATTGTTGGGTCTAGAGACTCTGAAGCTTTCTATATGATGAATCCTGATAGCAATGGTGTTCCTGAGCTTAGTATTTATTTGCTTAGAGTCTAG

Protein sequence

>MsG0280009234.01.T01

MARSSNMSSKRHFHWTNKVGTEDGEVHTSESFSTLIQKEDKKEADKVVDPEVSSVATASAHATRRKLRAVAISRLRSALTVFGKNRSNTPFGLGSRVVGTLFGYRRGHVHFAFQKDPTSQEAFLIELATPISGLVREMASGSVRIALECDKEKEAEKKSLRLLEEPQWRTYCNGKKCGFANRRECGQKEWDILKAVEPISMGAGVIPGTDNGAEAGQEGELMYMRAKFERIVGSRDSEAFYMMNPDSNGVPELSIYLLRV*