AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017372.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017372.01.T01 MTR_3g106140 97.436 156 4 0 1 156 197 352 2.97e-104 307
MsG0380017372.01.T01 MTR_1g014420 76.282 156 37 0 1 156 216 371 1.38e-74 231
MsG0380017372.01.T01 MTR_8g080280 36.170 141 80 3 20 156 533 667 1.64e-16 75.9
MsG0380017372.01.T01 MTR_5g079550 32.886 149 89 3 12 156 113 254 1.31e-14 70.1
MsG0380017372.01.T01 MTR_3g085610 34.375 160 93 5 1 156 514 665 3.28e-12 63.5
MsG0380017372.01.T01 MTR_4g485520 36.620 142 78 5 17 155 304 436 7.80e-12 62.4
MsG0380017372.01.T01 MTR_4g063800 34.532 139 81 4 17 153 452 582 7.92e-12 62.4
MsG0380017372.01.T01 MTR_4g485520 36.620 142 78 5 17 155 472 604 7.97e-12 62.4
MsG0380017372.01.T01 MTR_2g015870 39.362 94 57 0 63 156 227 320 9.30e-12 62.0
MsG0380017372.01.T01 MTR_5g069340 37.008 127 70 2 14 136 131 251 1.11e-11 61.6
MsG0380017372.01.T01 MTR_3g067685 34.862 109 61 2 32 136 154 256 1.23e-11 61.6
MsG0380017372.01.T01 MTR_7g059405 32.353 136 82 3 1 136 447 572 5.42e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017372.01.T01 AT4G31890 72.436 156 41 1 1 156 233 386 2.40e-75 233
MsG0380017372.01.T01 AT4G31890 72.436 156 41 1 1 156 233 386 2.40e-75 233
MsG0380017372.01.T01 AT4G31890 72.436 156 41 1 1 156 233 386 2.40e-75 233
MsG0380017372.01.T01 AT2G25130 68.590 156 47 1 1 156 200 353 4.56e-69 216
MsG0380017372.01.T01 AT1G67530 37.681 138 75 4 23 156 534 664 1.07e-15 73.6
MsG0380017372.01.T01 AT1G67530 37.681 138 75 4 23 156 534 664 1.07e-15 73.6
MsG0380017372.01.T01 AT1G67530 37.681 138 75 4 23 156 534 664 1.07e-15 73.6
MsG0380017372.01.T01 AT1G24330 36.765 136 78 3 23 156 534 663 7.76e-14 68.2
MsG0380017372.01.T01 AT1G24330 36.765 136 78 3 23 156 534 663 7.76e-14 68.2
MsG0380017372.01.T01 AT2G28830 35.252 139 80 4 17 153 386 516 1.76e-13 67.4
MsG0380017372.01.T01 AT2G23140 33.103 145 88 4 13 156 449 585 7.09e-13 65.5
MsG0380017372.01.T01 AT2G23140 33.103 145 88 4 13 156 449 585 7.09e-13 65.5
MsG0380017372.01.T01 AT2G23140 33.103 145 88 4 13 156 641 777 7.24e-13 65.5
MsG0380017372.01.T01 AT2G23140 33.103 145 88 4 13 156 638 774 7.30e-13 65.5
MsG0380017372.01.T01 AT2G23140 33.103 145 88 4 13 156 638 774 7.30e-13 65.5
MsG0380017372.01.T01 AT2G27430 31.293 147 97 1 10 156 172 314 1.73e-11 61.2

Find 24 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGGATGCAATCACAATTT+TGG 0.171187 3:-95456119 MsG0380017372.01.T01:CDS
GATTCTAACAAACCAATAAT+TGG 0.187713 3:-95456406 MsG0380017372.01.T01:CDS
GACGAATATTTCATTTGTTT+TGG 0.222706 3:-95456263 MsG0380017372.01.T01:CDS
GCACCAGAGGATCCAATTAT+TGG 0.223709 3:+95456394 None:intergenic
GAAGCCTTCTCTTGACATTC+AGG 0.257804 3:+95456070 None:intergenic
CCTTTCAATTCTGAGCAATT+TGG 0.260209 3:-95456179 MsG0380017372.01.T01:CDS
AAAAGCTATTAGTGCTGTTA+AGG 0.376170 3:-95456137 MsG0380017372.01.T01:CDS
TTTCCTTGGATTAAGTGCTT+TGG 0.382622 3:-95456428 MsG0380017372.01.T01:CDS
AAGTTAAACAAGATGCTCTT+CGG 0.386930 3:-95456312 MsG0380017372.01.T01:CDS
GCAATTGTTGCAAATTTCCT+TGG 0.402106 3:-95456442 MsG0380017372.01.T01:CDS
AGTTAAACAAGATGCTCTTC+GGG 0.453105 3:-95456311 MsG0380017372.01.T01:CDS
ACCTTGTGTTGTTGATTCAT+CGG 0.459932 3:-95456473 MsG0380017372.01.T01:CDS
AAACCAATAATTGGATCCTC+TGG 0.464190 3:-95456397 MsG0380017372.01.T01:CDS
TTTCAGAGTTCTAACTAGGA+AGG 0.499120 3:+95456366 None:intergenic
TTCAGAGTTCTAACTAGGAA+GGG 0.526621 3:+95456367 None:intergenic
CCAAATTGCTCAGAATTGAA+AGG 0.568365 3:+95456179 None:intergenic
TGTTGATTCATCGGTTTCTG+AGG 0.570602 3:-95456464 MsG0380017372.01.T01:CDS
AATCCAAAGCACTTAATCCA+AGG 0.592090 3:+95456425 None:intergenic
TTCACCTGAATGTCAAGAGA+AGG 0.606603 3:-95456074 MsG0380017372.01.T01:CDS
CAATTTGGTGTCGAGTCCCG+AGG 0.611390 3:-95456164 MsG0380017372.01.T01:CDS
TAGGAAGGGTATTGCACCAG+AGG 0.641343 3:+95456381 None:intergenic
GATAAACTCGATAGAAGACA+TGG 0.646492 3:-95456218 MsG0380017372.01.T01:CDS
ACCGATGAATCAACAACACA+AGG 0.656484 3:+95456472 None:intergenic
AATTTGGTGTCGAGTCCCGA+GGG 0.656713 3:-95456163 MsG0380017372.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCATACATTTTGATGATTA+TGG - Chr3:95456488-95456507 MsG0380017372.01.T01:CDS 20.0%
! GATTCTAACAAACCAATAAT+TGG - Chr3:95456132-95456151 MsG0380017372.01.T01:CDS 25.0%
!! GACGAATATTTCATTTGTTT+TGG - Chr3:95456275-95456294 MsG0380017372.01.T01:CDS 25.0%
!!! AATTTTTCAGAGTTCTAACT+AGG + Chr3:95456179-95456198 None:intergenic 25.0%
!!! ATTTGTTTTGGAAACTGATA+TGG - Chr3:95456287-95456306 MsG0380017372.01.T01:CDS 25.0%
!!! TTTTGGTTGATGTATTGAAT+TGG - Chr3:95456436-95456455 MsG0380017372.01.T01:CDS 25.0%
AAGTTAAACAAGATGCTCTT+CGG - Chr3:95456226-95456245 MsG0380017372.01.T01:CDS 30.0%
! AAAAGCTATTAGTGCTGTTA+AGG - Chr3:95456401-95456420 MsG0380017372.01.T01:CDS 30.0%
! TAAGGATGCAATCACAATTT+TGG - Chr3:95456419-95456438 MsG0380017372.01.T01:CDS 30.0%
AAACCAATAATTGGATCCTC+TGG - Chr3:95456141-95456160 MsG0380017372.01.T01:CDS 35.0%
AGTTAAACAAGATGCTCTTC+GGG - Chr3:95456227-95456246 MsG0380017372.01.T01:CDS 35.0%
CCAAATTGCTCAGAATTGAA+AGG + Chr3:95456362-95456381 None:intergenic 35.0%
CCTTTCAATTCTGAGCAATT+TGG - Chr3:95456359-95456378 MsG0380017372.01.T01:CDS 35.0%
GATAAACTCGATAGAAGACA+TGG - Chr3:95456320-95456339 MsG0380017372.01.T01:CDS 35.0%
GCAATTGTTGCAAATTTCCT+TGG - Chr3:95456096-95456115 MsG0380017372.01.T01:CDS 35.0%
TTTCCTTGGATTAAGTGCTT+TGG - Chr3:95456110-95456129 MsG0380017372.01.T01:CDS 35.0%
! AATCCAAAGCACTTAATCCA+AGG + Chr3:95456116-95456135 None:intergenic 35.0%
! ACTAATAGCTTTTCTACCCT+CGG + Chr3:95456394-95456413 None:intergenic 35.0%
! TTCAGAGTTCTAACTAGGAA+GGG + Chr3:95456174-95456193 None:intergenic 35.0%
! TTTCAGAGTTCTAACTAGGA+AGG + Chr3:95456175-95456194 None:intergenic 35.0%
!! ACCTTGTGTTGTTGATTCAT+CGG - Chr3:95456065-95456084 MsG0380017372.01.T01:CDS 35.0%
ACCGATGAATCAACAACACA+AGG + Chr3:95456069-95456088 None:intergenic 40.0%
TTCACCTGAATGTCAAGAGA+AGG - Chr3:95456464-95456483 MsG0380017372.01.T01:CDS 40.0%
! CTAATAGCTTTTCTACCCTC+GGG + Chr3:95456393-95456412 None:intergenic 40.0%
!! TGTTGATTCATCGGTTTCTG+AGG - Chr3:95456074-95456093 MsG0380017372.01.T01:CDS 40.0%
GAAGCCTTCTCTTGACATTC+AGG + Chr3:95456471-95456490 None:intergenic 45.0%
GCACCAGAGGATCCAATTAT+TGG + Chr3:95456147-95456166 None:intergenic 45.0%
!! AATTTGGTGTCGAGTCCCGA+GGG - Chr3:95456375-95456394 MsG0380017372.01.T01:CDS 50.0%
!! TAGGAAGGGTATTGCACCAG+AGG + Chr3:95456160-95456179 None:intergenic 50.0%
!! CAATTTGGTGTCGAGTCCCG+AGG - Chr3:95456374-95456393 MsG0380017372.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr3 gene 95456045 95456515 95456045 ID=MsG0380017372.01;Name=MsG0380017372.01
Chr3 mRNA 95456045 95456515 95456045 ID=MsG0380017372.01.T01;Parent=MsG0380017372.01;Name=MsG0380017372.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|156
Chr3 exon 95456045 95456515 95456045 ID=MsG0380017372.01.T01:exon:2152;Parent=MsG0380017372.01.T01
Chr3 CDS 95456045 95456515 95456045 ID=MsG0380017372.01.T01:cds;Parent=MsG0380017372.01.T01
Gene Sequence

>MsG0380017372.01.T01

ATGCTGAAGCTGATTGAATCACCTTGTGTTGTTGATTCATCGGTTTCTGAGGCAATTGTTGCAAATTTCCTTGGATTAAGTGCTTTGGATTCTAACAAACCAATAATTGGATCCTCTGGTGCAATACCCTTCCTAGTTAGAACTCTGAAAAATTTAGATAATAGTAGTAAAAGTAGTTCTCAAGTTAAACAAGATGCTCTTCGGGCTCTTTACAATCTATCGATCAATCAGACGAATATTTCATTTGTTTTGGAAACTGATATGGTCGCGTTTTTGATAAACTCGATAGAAGACATGGAAGTGAGTGAGAGAGTCCTTTCAATTCTGAGCAATTTGGTGTCGAGTCCCGAGGGTAGAAAAGCTATTAGTGCTGTTAAGGATGCAATCACAATTTTGGTTGATGTATTGAATTGGACAGATTCACCTGAATGTCAAGAGAAGGCTTCATACATTTTGATGATTATGGCATAA

Protein sequence

>MsG0380017372.01.T01

MLKLIESPCVVDSSVSEAIVANFLGLSALDSNKPIIGSSGAIPFLVRTLKNLDNSSKSSSQVKQDALRALYNLSINQTNISFVLETDMVAFLINSIEDMEVSERVLSILSNLVSSPEGRKAISAVKDAITILVDVLNWTDSPECQEKASYILMIMA*