AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021111.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021111.01.T01 MTR_4g066170 90.164 122 12 0 1 122 3 124 8.52e-73 233
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 30 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTCTTAACTTTGGAATTT+AGG 0.193046 4:-53621702 MsG0480021111.01.T01:CDS
ACAACCTCCTTTGTTTCTTT+TGG 0.222665 4:+53621947 None:intergenic
AGAAACAAAGGAGGTTGTTT+AGG 0.305076 4:-53621942 MsG0480021111.01.T01:CDS
TTGTTCAAGATGAGACAATT+TGG 0.358818 4:+53622042 None:intergenic
CAAAGTTAAGAGTGACTTAC+CGG 0.370974 4:+53621711 None:intergenic
ATCCAACATGAAAACATGTT+TGG 0.381038 4:+53621992 None:intergenic
TGATGACATAACAGATTTGT+TGG 0.393027 4:-53621776 MsG0480021111.01.T01:CDS
CTTAACTTTGGAATTTAGGT+TGG 0.397284 4:-53621698 MsG0480021111.01.T01:CDS
GTTGGGTAAATCAATTCTCT+TGG 0.421329 4:-53621758 MsG0480021111.01.T01:CDS
TTAACTTTGGAATTTAGGTT+GGG 0.425917 4:-53621697 MsG0480021111.01.T01:CDS
GATGACATAACAGATTTGTT+GGG 0.448090 4:-53621775 MsG0480021111.01.T01:CDS
AAGCCATGTCTCCATGGTAT+TGG 0.449477 4:-53622021 MsG0480021111.01.T01:CDS
CGGTAAGTCACTCTTAACTT+TGG 0.456895 4:-53621710 MsG0480021111.01.T01:CDS
AACAAAGGAGGTTGTTTAGG+AGG 0.475654 4:-53621939 MsG0480021111.01.T01:CDS
GTTAGCTCCGAGCTAGATCC+CGG 0.491907 4:-53621730 MsG0480021111.01.T01:CDS
ATGTTTCAACCAACAGTTGG+AGG 0.526379 4:-53621832 MsG0480021111.01.T01:CDS
GAGAAAAGCCCTCCAACTGT+TGG 0.528736 4:+53621823 None:intergenic
TCAGAAATGGCTATAGAAAG+AGG 0.531378 4:-53621802 MsG0480021111.01.T01:CDS
TTTGGTTACTTCCAATACCA+TGG 0.556225 4:+53622010 None:intergenic
CTTCCAATACCATGGAGACA+TGG 0.561407 4:+53622018 None:intergenic
GTAATGTTTCAACCAACAGT+TGG 0.572656 4:-53621835 MsG0480021111.01.T01:CDS
TGACTTACCGGGATCTAGCT+CGG 0.577812 4:+53621723 None:intergenic
CAGAAATGGCTATAGAAAGA+GGG 0.580132 4:-53621801 MsG0480021111.01.T01:CDS
TTGAACAAGCCATGTCTCCA+TGG 0.586444 4:-53622027 MsG0480021111.01.T01:CDS
TGTTTCAACCAACAGTTGGA+GGG 0.612746 4:-53621831 MsG0480021111.01.T01:CDS
TCATAAACCAAAAGAAACAA+AGG 0.629237 4:-53621954 MsG0480021111.01.T01:CDS
AAAGTTAAGAGTGACTTACC+GGG 0.662982 4:+53621712 None:intergenic
TAAACCAAAAGAAACAAAGG+AGG 0.689233 4:-53621951 MsG0480021111.01.T01:CDS
TGTTGGTTGAAACATTACTG+TGG 0.705092 4:+53621840 None:intergenic
GTGCAGCAAAATCAAAGCTG+TGG 0.713390 4:-53621911 MsG0480021111.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TCATAAACCAAAAGAAACAA+AGG - Chr4:53621793-53621812 MsG0480021111.01.T01:CDS 25.0%
!!! TTAACTTTGGAATTTAGGTT+GGG - Chr4:53622050-53622069 MsG0480021111.01.T01:CDS 25.0%
!!! TTCTTTTGGTTTATGAACAA+AGG + Chr4:53621789-53621808 None:intergenic 25.0%
ATCCAACATGAAAACATGTT+TGG + Chr4:53621758-53621777 None:intergenic 30.0%
TAAACCAAAAGAAACAAAGG+AGG - Chr4:53621796-53621815 MsG0480021111.01.T01:CDS 30.0%
TGATGACATAACAGATTTGT+TGG - Chr4:53621971-53621990 MsG0480021111.01.T01:CDS 30.0%
TTGTTCAAGATGAGACAATT+TGG + Chr4:53621708-53621727 None:intergenic 30.0%
! AACCAAACATGTTTTCATGT+TGG - Chr4:53621753-53621772 MsG0480021111.01.T01:CDS 30.0%
! GATGACATAACAGATTTGTT+GGG - Chr4:53621972-53621991 MsG0480021111.01.T01:CDS 30.0%
!! CACTCTTAACTTTGGAATTT+AGG - Chr4:53622045-53622064 MsG0480021111.01.T01:CDS 30.0%
!! CTTAACTTTGGAATTTAGGT+TGG - Chr4:53622049-53622068 MsG0480021111.01.T01:CDS 30.0%
AAAGTTAAGAGTGACTTACC+GGG + Chr4:53622038-53622057 None:intergenic 35.0%
AGAAACAAAGGAGGTTGTTT+AGG - Chr4:53621805-53621824 MsG0480021111.01.T01:CDS 35.0%
CAAAGTTAAGAGTGACTTAC+CGG + Chr4:53622039-53622058 None:intergenic 35.0%
CAGAAATGGCTATAGAAAGA+GGG - Chr4:53621946-53621965 MsG0480021111.01.T01:CDS 35.0%
GTAATGTTTCAACCAACAGT+TGG - Chr4:53621912-53621931 MsG0480021111.01.T01:CDS 35.0%
GTTGGGTAAATCAATTCTCT+TGG - Chr4:53621989-53622008 MsG0480021111.01.T01:CDS 35.0%
TCAGAAATGGCTATAGAAAG+AGG - Chr4:53621945-53621964 MsG0480021111.01.T01:CDS 35.0%
TTTGGTTACTTCCAATACCA+TGG + Chr4:53621740-53621759 None:intergenic 35.0%
! TGTTGGTTGAAACATTACTG+TGG + Chr4:53621910-53621929 None:intergenic 35.0%
!! ACAACCTCCTTTGTTTCTTT+TGG + Chr4:53621803-53621822 None:intergenic 35.0%
AACAAAGGAGGTTGTTTAGG+AGG - Chr4:53621808-53621827 MsG0480021111.01.T01:CDS 40.0%
ATGTTTCAACCAACAGTTGG+AGG - Chr4:53621915-53621934 MsG0480021111.01.T01:CDS 40.0%
CGGTAAGTCACTCTTAACTT+TGG - Chr4:53622037-53622056 MsG0480021111.01.T01:CDS 40.0%
TGTTTCAACCAACAGTTGGA+GGG - Chr4:53621916-53621935 MsG0480021111.01.T01:CDS 40.0%
! GTTTTCATGTTGGATCGTCA+AGG - Chr4:53621763-53621782 MsG0480021111.01.T01:CDS 40.0%
AAGCCATGTCTCCATGGTAT+TGG - Chr4:53621726-53621745 MsG0480021111.01.T01:CDS 45.0%
CTTCCAATACCATGGAGACA+TGG + Chr4:53621732-53621751 None:intergenic 45.0%
GTGCAGCAAAATCAAAGCTG+TGG - Chr4:53621836-53621855 MsG0480021111.01.T01:CDS 45.0%
TTGAACAAGCCATGTCTCCA+TGG - Chr4:53621720-53621739 MsG0480021111.01.T01:CDS 45.0%
! TGGAGGGCTTTTCTCAGAAA+TGG - Chr4:53621932-53621951 MsG0480021111.01.T01:CDS 45.0%
GAGAAAAGCCCTCCAACTGT+TGG + Chr4:53621927-53621946 None:intergenic 50.0%
TGACTTACCGGGATCTAGCT+CGG + Chr4:53622027-53622046 None:intergenic 50.0%
GTTAGCTCCGAGCTAGATCC+CGG - Chr4:53622017-53622036 MsG0480021111.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 53621684 53622085 53621684 ID=MsG0480021111.01;Name=MsG0480021111.01
Chr4 mRNA 53621684 53622085 53621684 ID=MsG0480021111.01.T01;Parent=MsG0480021111.01;Name=MsG0480021111.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|133
Chr4 exon 53621684 53622085 53621684 ID=MsG0480021111.01.T01:exon:28700;Parent=MsG0480021111.01.T01
Chr4 CDS 53621684 53622085 53621684 ID=MsG0480021111.01.T01:cds;Parent=MsG0480021111.01.T01
Gene Sequence

>MsG0480021111.01.T01

ATGCAACAAATAAGTTTAAAGCCAAATTGTCTCATCTTGAACAAGCCATGTCTCCATGGTATTGGAAGTAACCAAACATGTTTTCATGTTGGATCGTCAAGGCCTTTGTTCATAAACCAAAAGAAACAAAGGAGGTTGTTTAGGAGGAATGAGTGCAGCAAAATCAAAGCTGTGGCTGTGAGTGATGAAGAACTTATAGAACAGCAATCAGTAAAAGTAAAAGCCACAGTAATGTTTCAACCAACAGTTGGAGGGCTTTTCTCAGAAATGGCTATAGAAAGAGGGCTTGATGACATAACAGATTTGTTGGGTAAATCAATTCTCTTGGAGTTTGTTAGCTCCGAGCTAGATCCCGGTAAGTCACTCTTAACTTTGGAATTTAGGTTGGGTGCAATAAGATAG

Protein sequence

>MsG0480021111.01.T01

MQQISLKPNCLILNKPCLHGIGSNQTCFHVGSSRPLFINQKKQRRLFRRNECSKIKAVAVSDEELIEQQSVKVKATVMFQPTVGGLFSEMAIERGLDDITDLLGKSILLEFVSSELDPGKSLLTLEFRLGAIR*