AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580028397.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028397.01.T01 MTR_5g070210 96.599 147 5 0 13 159 164 310 2.43e-103 298
MsG0580028397.01.T01 MTR_5g070220 80.142 141 28 0 19 159 1 141 1.68e-84 244
MsG0580028397.01.T01 MTR_5g070250 77.241 145 33 0 13 157 79 223 1.50e-81 240
MsG0580028397.01.T01 MTR_3g074950 62.921 89 32 1 71 158 2 90 1.99e-35 119
MsG0580028397.01.T01 MTR_8g073540 43.284 67 38 0 86 152 284 350 7.36e-12 62.4
MsG0580028397.01.T01 MTR_1g095500 50.000 54 27 0 99 152 297 350 8.94e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028397.01.T01 AT5G58770 70.139 144 43 0 15 158 165 308 4.06e-74 224
MsG0580028397.01.T01 AT5G58780 58.904 146 60 0 13 158 156 301 4.94e-60 188
MsG0580028397.01.T01 AT5G58782 57.639 144 61 0 15 158 145 288 1.62e-59 186
MsG0580028397.01.T01 AT5G58782 57.639 144 61 0 15 158 163 306 2.33e-59 186
MsG0580028397.01.T01 AT2G23410 56.944 144 62 0 15 158 159 302 9.67e-58 182
MsG0580028397.01.T01 AT5G58784 61.429 140 52 1 9 148 154 291 5.24e-56 178
MsG0580028397.01.T01 AT2G23400 49.306 144 58 2 15 158 158 286 1.77e-42 143
MsG0580028397.01.T01 AT2G17570 29.193 161 82 2 14 149 129 282 2.05e-16 74.7
MsG0580028397.01.T01 AT5G60500 30.556 144 88 3 14 152 125 261 1.58e-15 72.0
MsG0580028397.01.T01 AT5G60510 29.861 144 89 3 14 152 126 262 1.56e-14 69.3

Find 30 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAAACTTTGGCCAGATTT+TGG 0.177145 5:-79954879 MsG0580028397.01.T01:CDS
TGCCAAAATAAGATGGAATT+TGG 0.186602 5:+79955133 None:intergenic
TACCAAATTCCATCTTATTT+TGG 0.187683 5:-79955135 MsG0580028397.01.T01:CDS
AATGCCATCTTGCACTTTCT+TGG 0.210412 5:+79955061 None:intergenic
ATCGGAACGGCGACAACAAC+CGG 0.308340 5:+79955502 None:intergenic
TAATTTCTTGTTATGGCAAT+TGG 0.324656 5:-79954925 MsG0580028397.01.T01:CDS
GGGTTAGTAATTTCTTGTTA+TGG 0.341933 5:-79954932 MsG0580028397.01.T01:CDS
GCAAGATGGCATTATTCATT+TGG 0.346742 5:-79955051 MsG0580028397.01.T01:CDS
AACTCATCCTTTCCAAAATC+TGG 0.362218 5:+79954867 None:intergenic
CTGGTCCGTATTAGTAAATC+CGG 0.368742 5:+79954966 None:intergenic
CAAAATAGACAGAGACGTTA+TGG 0.385865 5:-79954822 MsG0580028397.01.T01:CDS
CTTACCCGGATTTACTAATA+CGG 0.419675 5:-79954971 MsG0580028397.01.T01:CDS
CAATGAGAGATGGCGGTTGC+CGG 0.428024 5:-79955521 None:intergenic
AAATGAAAATATTATTGAAA+AGG 0.447113 5:-79955021 MsG0580028397.01.T01:CDS
GGAATTCAAATATCTGTGAT+TGG 0.460356 5:-79955227 MsG0580028397.01.T01:CDS
ACGGACCAGTGGAGAACTTA+GGG 0.473431 5:-79954952 MsG0580028397.01.T01:CDS
GGTTGTTGTCGCCGTTCCGA+TGG 0.485209 5:-79955500 MsG0580028397.01.T01:CDS
AGCTTACTGCCAAAATAAGA+TGG 0.496898 5:+79955126 None:intergenic
CGTATTAGTAAATCCGGGTA+AGG 0.505626 5:+79954972 None:intergenic
TACGGACCAGTGGAGAACTT+AGG 0.513930 5:-79954953 MsG0580028397.01.T01:CDS
GTTGTTGTCGCCGTTCCGAT+GGG 0.532247 5:-79955499 MsG0580028397.01.T01:intron
TTGTACAGAATTTCCTTACC+CGG 0.560525 5:-79954985 MsG0580028397.01.T01:CDS
ACTAACCCTAAGTTCTCCAC+TGG 0.570013 5:+79954947 None:intergenic
ATTTCTCCCACCCATCGGAA+CGG 0.625418 5:+79955489 None:intergenic
TAGTGCTGAGGAGAGTACAA+AGG 0.639099 5:-79955162 MsG0580028397.01.T01:CDS
GTAGCCAAGAAAGTGCAAGA+TGG 0.652351 5:-79955065 MsG0580028397.01.T01:CDS
AATAGACAGAGACGTTATGG+TGG 0.652580 5:-79954819 MsG0580028397.01.T01:CDS
TGGTCCGTATTAGTAAATCC+GGG 0.652721 5:+79954967 None:intergenic
AAGTATGATAGCTAGTGCTG+AGG 0.671510 5:-79955174 MsG0580028397.01.T01:CDS
GATTTACTAATACGGACCAG+TGG 0.708808 5:-79954963 MsG0580028397.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATGAAAATATTATTGAAA+AGG - Chr5:79955300-79955319 MsG0580028397.01.T01:intron 10.0%
!! TGGTATGAAAAAAAAAAAAT+CGG + Chr5:79954905-79954924 None:intergenic 15.0%
!!! TTTTCTTTAATAGAAAACTT+TGG - Chr5:79955431-79955450 MsG0580028397.01.T01:intron 15.0%
!!! TCTATTTTGAAATGAAGTTA+AGG + Chr5:79955488-79955507 None:intergenic 20.0%
!! ATCATACTTTTCAATGACTT+GGG + Chr5:79955136-79955155 None:intergenic 25.0%
!! TAATTTCTTGTTATGGCAAT+TGG - Chr5:79955396-79955415 MsG0580028397.01.T01:intron 25.0%
!! TATCATACTTTTCAATGACT+TGG + Chr5:79955137-79955156 None:intergenic 25.0%
!!! TACCAAATTCCATCTTATTT+TGG - Chr5:79955186-79955205 MsG0580028397.01.T01:CDS 25.0%
AACACCATTACTTTGTTACA+GGG - Chr5:79955069-79955088 MsG0580028397.01.T01:CDS 30.0%
ATACCACTCCATATATATAG+GGG - Chr5:79954919-79954938 MsG0580028397.01.T01:CDS 30.0%
CATACCACTCCATATATATA+GGG - Chr5:79954918-79954937 MsG0580028397.01.T01:CDS 30.0%
GGAATTCAAATATCTGTGAT+TGG - Chr5:79955094-79955113 MsG0580028397.01.T01:CDS 30.0%
TAACACCATTACTTTGTTAC+AGG - Chr5:79955068-79955087 MsG0580028397.01.T01:CDS 30.0%
TCATACCACTCCATATATAT+AGG - Chr5:79954917-79954936 MsG0580028397.01.T01:CDS 30.0%
TGCCAAAATAAGATGGAATT+TGG + Chr5:79955191-79955210 None:intergenic 30.0%
! ATGCTTTTCCCCTATATATA+TGG + Chr5:79954930-79954949 None:intergenic 30.0%
! GGGTTAGTAATTTCTTGTTA+TGG - Chr5:79955389-79955408 MsG0580028397.01.T01:intron 30.0%
! TTTTACCATTTTACCCTTGT+GGG + Chr5:79954977-79954996 None:intergenic 30.0%
! TTTTTACCATTTTACCCTTG+TGG + Chr5:79954978-79954997 None:intergenic 30.0%
AACTCATCCTTTCCAAAATC+TGG + Chr5:79955457-79955476 None:intergenic 35.0%
AGAAAAATGAGAGAGACGAT+GGG - Chr5:79954859-79954878 MsG0580028397.01.T01:CDS 35.0%
AGCTTACTGCCAAAATAAGA+TGG + Chr5:79955198-79955217 None:intergenic 35.0%
CAAAATAGACAGAGACGTTA+TGG - Chr5:79955499-79955518 MsG0580028397.01.T01:intron 35.0%
CTTACCCGGATTTACTAATA+CGG - Chr5:79955350-79955369 MsG0580028397.01.T01:intron 35.0%
GCAAGATGGCATTATTCATT+TGG - Chr5:79955270-79955289 MsG0580028397.01.T01:intron 35.0%
TTGTACAGAATTTCCTTACC+CGG - Chr5:79955336-79955355 MsG0580028397.01.T01:intron 35.0%
TTTCCCCTATATATATGGAG+TGG + Chr5:79954925-79954944 None:intergenic 35.0%
! AGAAAACTTTGGCCAGATTT+TGG - Chr5:79955442-79955461 MsG0580028397.01.T01:intron 35.0%
! TTTACCATTTTACCCTTGTG+GGG + Chr5:79954976-79954995 None:intergenic 35.0%
!! ATTTTGGCAGTAAGCTATAG+TGG - Chr5:79955202-79955221 MsG0580028397.01.T01:CDS 35.0%
AAGTATGATAGCTAGTGCTG+AGG - Chr5:79955147-79955166 MsG0580028397.01.T01:CDS 40.0%
AATAGACAGAGACGTTATGG+TGG - Chr5:79955502-79955521 MsG0580028397.01.T01:CDS 40.0%
AATGCCATCTTGCACTTTCT+TGG + Chr5:79955263-79955282 None:intergenic 40.0%
CCATTACTTTGTTACAGGGA+AGG - Chr5:79955073-79955092 MsG0580028397.01.T01:CDS 40.0%
CCTTCCCTGTAACAAAGTAA+TGG + Chr5:79955076-79955095 None:intergenic 40.0%
CGTATTAGTAAATCCGGGTA+AGG + Chr5:79955352-79955371 None:intergenic 40.0%
CTGGTCCGTATTAGTAAATC+CGG + Chr5:79955358-79955377 None:intergenic 40.0%
GAGAAAAATGAGAGAGACGA+TGG - Chr5:79954858-79954877 MsG0580028397.01.T01:CDS 40.0%
GATTTACTAATACGGACCAG+TGG - Chr5:79955358-79955377 MsG0580028397.01.T01:intron 40.0%
TGGTCCGTATTAGTAAATCC+GGG + Chr5:79955357-79955376 None:intergenic 40.0%
TTACCAAAATACCCCCACAA+GGG - Chr5:79954961-79954980 MsG0580028397.01.T01:CDS 40.0%
TTTACCAAAATACCCCCACA+AGG - Chr5:79954960-79954979 MsG0580028397.01.T01:CDS 40.0%
! TTACCATTTTACCCTTGTGG+GGG + Chr5:79954975-79954994 None:intergenic 40.0%
!! ACTTTGGCCAGATTTTGGAA+AGG - Chr5:79955447-79955466 MsG0580028397.01.T01:intron 40.0%
ACTAACCCTAAGTTCTCCAC+TGG + Chr5:79955377-79955396 None:intergenic 45.0%
ATACCCCCACAAGGGTAAAA+TGG - Chr5:79954969-79954988 MsG0580028397.01.T01:CDS 45.0%
! GTAGCCAAGAAAGTGCAAGA+TGG - Chr5:79955256-79955275 MsG0580028397.01.T01:intron 45.0%
! TAGTGCTGAGGAGAGTACAA+AGG - Chr5:79955159-79955178 MsG0580028397.01.T01:CDS 45.0%
! TGGCTACACTTTTGCATGCT+TGG + Chr5:79955243-79955262 None:intergenic 45.0%
!!! TTACCCTTGTGGGGGTATTT+TGG + Chr5:79954967-79954986 None:intergenic 45.0%
ACGGACCAGTGGAGAACTTA+GGG - Chr5:79955369-79955388 MsG0580028397.01.T01:intron 50.0%
ATTTCTCCCACCCATCGGAA+CGG + Chr5:79954835-79954854 None:intergenic 50.0%
TACGGACCAGTGGAGAACTT+AGG - Chr5:79955368-79955387 MsG0580028397.01.T01:intron 50.0%
TCTGCATTTCTCCCACCCAT+CGG + Chr5:79954840-79954859 None:intergenic 50.0%
ATCGGAACGGCGACAACAAC+CGG + Chr5:79954822-79954841 None:intergenic 55.0%
GTTGTTGTCGCCGTTCCGAT+GGG - Chr5:79954822-79954841 MsG0580028397.01.T01:CDS 55.0%
GGTTGTTGTCGCCGTTCCGA+TGG - Chr5:79954821-79954840 MsG0580028397.01.T01:CDS 60.0%
TTGTCGCCGTTCCGATGGGT+GGG - Chr5:79954826-79954845 MsG0580028397.01.T01:CDS 60.0%
GTTGTCGCCGTTCCGATGGG+TGG - Chr5:79954825-79954844 MsG0580028397.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 79954809 79955534 79954809 ID=MsG0580028397.01;Name=MsG0580028397.01
Chr5 mRNA 79954809 79955534 79954809 ID=MsG0580028397.01.T01;Parent=MsG0580028397.01;Name=MsG0580028397.01.T01;_AED=0.50;_eAED=0.54;_QI=0|0|0|1|0|0.5|2|0|159
Chr5 exon 79955500 79955534 79955500 ID=MsG0580028397.01.T01:exon:5763;Parent=MsG0580028397.01.T01
Chr5 exon 79954809 79955253 79954809 ID=MsG0580028397.01.T01:exon:5762;Parent=MsG0580028397.01.T01
Chr5 CDS 79955500 79955534 79955500 ID=MsG0580028397.01.T01:cds;Parent=MsG0580028397.01.T01
Chr5 CDS 79954809 79955253 79954809 ID=MsG0580028397.01.T01:cds;Parent=MsG0580028397.01.T01
Gene Sequence

>MsG0580028397.01.T01

ATGGCGGTTGCCGGTTGTTGTCGCCGTTCCGATGGGGAAGGAATTCAAATATCTGTGATTGGAGATACATCAAAGCTACCCAAGTCATTGAAAAGTATGATAGCTAGTGCTGAGGAGAGTACAAAGGATAATACCAAATTCCATCTTATTTTGGCAGTAAGCTATAGTGGAAAATACGATTTAGTCCAAGCATGCAAAAGTGTAGCCAAGAAAGTGCAAGATGGCATTATTCATTTGGAAGACATAAATGAAAATATTATTGAAAAGGAATTAGAAACTAATTGTACAGAATTTCCTTACCCGGATTTACTAATACGGACCAGTGGAGAACTTAGGGTTAGTAATTTCTTGTTATGGCAATTGGCATATACAGAACTTTTCTTTAATAGAAAACTTTGGCCAGATTTTGGAAAGGATGAGTTTGTAGAAGCCTTAACTTCATTTCAAAATAGACAGAGACGTTATGGTGGACGATATTAA

Protein sequence

>MsG0580028397.01.T01

MAVAGCCRRSDGEGIQISVIGDTSKLPKSLKSMIASAEESTKDNTKFHLILAVSYSGKYDLVQACKSVAKKVQDGIIHLEDINENIIEKELETNCTEFPYPDLLIRTSGELRVSNFLLWQLAYTELFFNRKLWPDFGKDEFVEALTSFQNRQRRYGGRY*