Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024583.01.T01 | XP_003611388.2 | 81.818 | 297 | 9 | 2 | 1 | 297 | 1 | 252 | 1.12E-171 | 485 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024583.01.T01 | O23547 | 52.465 | 284 | 87 | 5 | 12 | 294 | 11 | 247 | 6.06E-101 | 298 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024583.01.T01 | G7JYL2 | 81.818 | 297 | 9 | 2 | 1 | 297 | 1 | 252 | 5.33e-172 | 485 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048058.01 | MsG0580024583.01 | 0.804568 | 2.029803e-49 | 1.050067e-46 |
| MsG0180000235.01 | MsG0580024583.01 | 0.812882 | 3.389339e-51 | 2.180332e-48 |
| MsG0180001294.01 | MsG0580024583.01 | 0.817765 | 2.783168e-52 | 2.045152e-49 |
| MsG0180001840.01 | MsG0580024583.01 | 0.807809 | 4.216169e-50 | 2.371111e-47 |
| MsG0180002283.01 | MsG0580024583.01 | 0.810242 | 1.270556e-50 | 7.619261e-48 |
| MsG0180003190.01 | MsG0580024583.01 | 0.831735 | 1.410413e-55 | 1.544278e-52 |
| MsG0180003744.01 | MsG0580024583.01 | 0.807804 | 4.226583e-50 | 2.376659e-47 |
| MsG0180003860.01 | MsG0580024583.01 | 0.808997 | 2.352124e-50 | 1.364794e-47 |
| MsG0180004645.01 | MsG0580024583.01 | 0.805809 | 1.115925e-49 | 5.960494e-47 |
| MsG0180004730.01 | MsG0580024583.01 | 0.808660 | 2.776762e-50 | 1.596851e-47 |
| MsG0180005125.01 | MsG0580024583.01 | 0.849142 | 3.908992e-60 | 7.341380e-57 |
| MsG0180005398.01 | MsG0580024583.01 | 0.806521 | 7.901178e-50 | 4.297601e-47 |
| MsG0180005765.01 | MsG0580024583.01 | 0.810217 | 1.286553e-50 | 7.709758e-48 |
| MsG0180006016.01 | MsG0580024583.01 | 0.803078 | 4.139179e-49 | 2.060787e-46 |
| MsG0280006356.01 | MsG0580024583.01 | 0.805925 | 1.054726e-49 | 5.650456e-47 |
| MsG0280010614.01 | MsG0580024583.01 | 0.802571 | 5.266405e-49 | 2.588423e-46 |
| MsG0280010784.01 | MsG0580024583.01 | 0.804352 | 2.250969e-49 | 1.158051e-46 |
| MsG0280010901.01 | MsG0580024583.01 | 0.804033 | 2.622533e-49 | 1.338237e-46 |
| MsG0280011235.01 | MsG0580024583.01 | 0.805790 | 1.125832e-49 | 6.010621e-47 |
| MsG0380011729.01 | MsG0580024583.01 | 0.836764 | 7.728705e-57 | 9.836095e-54 |
| MsG0380012124.01 | MsG0580024583.01 | 0.805838 | 1.100053e-49 | 5.880163e-47 |
| MsG0380014365.01 | MsG0580024583.01 | 0.823413 | 1.404163e-53 | 1.208034e-50 |
| MsG0380014541.01 | MsG0580024583.01 | 0.819912 | 9.050277e-53 | 7.057546e-50 |
| MsG0380014885.01 | MsG0580024583.01 | 0.808072 | 3.705921e-50 | 2.098618e-47 |
| MsG0480018462.01 | MsG0580024583.01 | 0.812494 | 4.122601e-51 | 2.625167e-48 |
| MsG0480019404.01 | MsG0580024583.01 | 0.814358 | 1.604848e-51 | 1.074480e-48 |
| MsG0480020065.01 | MsG0580024583.01 | 0.812737 | 3.646236e-51 | 2.336729e-48 |
| MsG0480020372.01 | MsG0580024583.01 | 0.813364 | 2.657778e-51 | 1.732182e-48 |
| MsG0480020416.01 | MsG0580024583.01 | 0.825221 | 5.274844e-54 | 4.778579e-51 |
| MsG0480020445.01 | MsG0580024583.01 | 0.818178 | 2.244513e-52 | 1.668119e-49 |
| MsG0480020791.01 | MsG0580024583.01 | 0.826537 | 2.568790e-54 | 2.415795e-51 |
| MsG0480020843.01 | MsG0580024583.01 | 0.804436 | 2.162265e-49 | 1.114791e-46 |
| MsG0480021240.01 | MsG0580024583.01 | 0.810504 | 1.115407e-50 | 6.735282e-48 |
| MsG0480021695.01 | MsG0580024583.01 | 0.823678 | 1.217174e-53 | 1.055283e-50 |
| MsG0480022104.01 | MsG0580024583.01 | 0.809407 | 1.921144e-50 | 1.127077e-47 |
| MsG0480022108.01 | MsG0580024583.01 | 0.812328 | 4.480916e-51 | 2.840737e-48 |
| MsG0480023224.01 | MsG0580024583.01 | 0.808638 | 2.807043e-50 | 1.613321e-47 |
| MsG0480023245.01 | MsG0580024583.01 | 0.832150 | 1.113946e-55 | 1.234417e-52 |
| MsG0480023447.01 | MsG0580024583.01 | 0.836273 | 1.030539e-56 | 1.292473e-53 |
| MsG0480023517.01 | MsG0580024583.01 | 0.819869 | 9.260899e-53 | 7.212716e-50 |
| MsG0580024583.01 | MsG0580025134.01 | 0.814406 | 1.566243e-51 | 1.050080e-48 |
| MsG0580024583.01 | MsG0580025989.01 | 0.830812 | 2.377786e-55 | 2.532971e-52 |
| MsG0580024583.01 | MsG0580029658.01 | 0.806488 | 8.029057e-50 | 4.363491e-47 |
| MsG0580024583.01 | MsG0680030436.01 | 0.821416 | 4.086066e-53 | 3.322171e-50 |
| MsG0580024583.01 | MsG0680030684.01 | 0.826244 | 3.017608e-54 | 2.814404e-51 |
| MsG0580024583.01 | MsG0680030685.01 | 0.820544 | 6.486690e-53 | 5.147588e-50 |
| MsG0580024583.01 | MsG0680030841.01 | 0.833981 | 3.901707e-56 | 4.567616e-53 |
| MsG0580024583.01 | MsG0680031133.01 | 0.802084 | 6.635270e-49 | 3.221107e-46 |
| MsG0580024583.01 | MsG0680031651.01 | 0.816068 | 6.692053e-52 | 4.694258e-49 |
| MsG0580024583.01 | MsG0680034061.01 | 0.805513 | 1.287243e-49 | 6.823112e-47 |
| MsG0580024583.01 | MsG0780037415.01 | 0.801156 | 1.028592e-48 | 4.877451e-46 |
| MsG0580024583.01 | MsG0780037503.01 | 0.833893 | 4.106119e-56 | 4.793962e-53 |
| MsG0580024583.01 | MsG0780039452.01 | 0.835213 | 1.912330e-56 | 2.322961e-53 |
| MsG0580024583.01 | MsG0780039570.01 | 0.821215 | 4.544862e-53 | 3.674881e-50 |
| MsG0580024583.01 | MsG0780039587.01 | 0.805409 | 1.353906e-49 | 7.157506e-47 |
| MsG0580024583.01 | MsG0780039950.01 | 0.818021 | 2.436397e-52 | 1.802838e-49 |
| MsG0580024583.01 | MsG0780040845.01 | 0.801046 | 1.083074e-48 | 5.121801e-46 |
| MsG0580024583.01 | MsG0780040861.01 | 0.826717 | 2.327517e-54 | 2.200131e-51 |
| MsG0580024583.01 | MsG0780040929.01 | 0.802046 | 6.755892e-49 | 3.276428e-46 |
| MsG0580024583.01 | MsG0780041065.01 | 0.807408 | 5.130032e-50 | 2.855204e-47 |
| MsG0580024583.01 | MsG0780041255.01 | 0.810820 | 9.531295e-51 | 5.804363e-48 |
| MsG0580024583.01 | MsG0780041376.01 | 0.807115 | 5.917708e-50 | 3.268389e-47 |
| MsG0580024583.01 | MsG0780041469.01 | 0.830131 | 3.489353e-55 | 3.643913e-52 |
| MsG0580024583.01 | MsG0880042151.01 | 0.804417 | 2.181703e-49 | 1.124258e-46 |
| MsG0580024583.01 | MsG0880042206.01 | 0.808314 | 3.292199e-50 | 1.876322e-47 |
| MsG0580024583.01 | MsG0880042811.01 | 0.828361 | 9.385331e-55 | 9.307315e-52 |
| MsG0580024583.01 | MsG0880043082.01 | 0.811243 | 7.717445e-51 | 4.752590e-48 |
| MsG0580024583.01 | MsG0880043532.01 | 0.809860 | 1.535430e-50 | 9.116265e-48 |
| MsG0580024583.01 | MsG0880044075.01 | 0.837255 | 5.790734e-57 | 7.478847e-54 |
| MsG0580024583.01 | MsG0880045106.01 | 0.824400 | 8.241279e-54 | 7.291336e-51 |
| MsG0580024583.01 | MsG0880045631.01 | 0.829279 | 5.624627e-55 | 5.731028e-52 |
| MsG0580024583.01 | MsG0880045914.01 | 0.800195 | 1.615183e-48 | 7.474317e-46 |
| MsG0580024583.01 | MsG0880046047.01 | 0.827827 | 1.261718e-54 | 1.231869e-51 |
| MsG0580024583.01 | MsG0880046113.01 | 0.849411 | 3.289843e-60 | 6.230883e-57 |
| MsG0580024583.01 | MsG0880046317.01 | 0.847282 | 1.275966e-59 | 2.256510e-56 |
| MsG0580024583.01 | MsG0880046684.01 | 0.813304 | 2.738592e-51 | 1.781901e-48 |
| MsG0580024583.01 | MsG0880046762.01 | 0.841859 | 3.680317e-58 | 5.479253e-55 |
| MsG0580024583.01 | MsG0880047016.01 | 0.816915 | 4.322136e-52 | 3.102880e-49 |
| MsG0580024583.01 | MsG0880047191.01 | 0.801064 | 1.073681e-48 | 5.079652e-46 |
| MsG0580024583.01 | MsG0880047391.01 | 0.804304 | 2.303703e-49 | 1.183706e-46 |
| MsG0580024583.01 | MsG0880047590.01 | 0.854169 | 1.471615e-61 | 3.258224e-58 |
| MsG0580024583.01 | MsG0880047607.01 | 0.801120 | 1.045824e-48 | 4.954754e-46 |
| MsG0580024583.01 | MsG0880047742.01 | 0.812379 | 4.366198e-51 | 2.771773e-48 |
| MsG0380015607.01 | MsG0580024583.01 | 0.818024 | 2.432547e-52 | 1.800146e-49 |
| MsG0380016055.01 | MsG0580024583.01 | 0.851552 | 8.237090e-61 | 1.673420e-57 |
| MsG0380016227.01 | MsG0580024583.01 | 0.807678 | 4.495342e-50 | 2.519458e-47 |
| MsG0380016985.01 | MsG0580024583.01 | 0.809596 | 1.749983e-50 | 1.031836e-47 |
| MsG0380017226.01 | MsG0580024583.01 | 0.805009 | 1.641714e-49 | 8.590191e-47 |
| MsG0280006501.01 | MsG0580024583.01 | 0.826453 | 2.690618e-54 | 2.524394e-51 |
| MsG0280006728.01 | MsG0580024583.01 | 0.809846 | 1.546646e-50 | 9.179437e-48 |
| MsG0280007549.01 | MsG0580024583.01 | 0.816773 | 4.651949e-52 | 3.326692e-49 |
| MsG0280007648.01 | MsG0580024583.01 | 0.823443 | 1.381492e-53 | 1.189628e-50 |
| MsG0280008286.01 | MsG0580024583.01 | 0.851348 | 9.412091e-61 | 1.899680e-57 |
| MsG0280008502.01 | MsG0580024583.01 | 0.806549 | 7.794209e-50 | 4.242565e-47 |
| MsG0280008885.01 | MsG0580024583.01 | 0.803386 | 3.573933e-49 | 1.793536e-46 |
| MsG0280008907.01 | MsG0580024583.01 | 0.847291 | 1.269379e-59 | 2.245365e-56 |
| MsG0280008970.01 | MsG0580024583.01 | 0.827079 | 1.907525e-54 | 1.822357e-51 |
| MsG0280010389.01 | MsG0580024583.01 | 0.804644 | 1.956813e-49 | 1.014304e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0580024583.01 | MsG0480021761.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024583.01.T01 | MTR_5g013440 | 81.818 | 297 | 9 | 2 | 1 | 297 | 1 | 252 | 1.35e-175 | 485 |
| MsG0580024583.01.T01 | MTR_4g099370 | 63.176 | 296 | 64 | 2 | 2 | 297 | 1 | 251 | 1.03e-137 | 389 |
| MsG0580024583.01.T01 | MTR_4g099400 | 51.010 | 198 | 68 | 1 | 98 | 295 | 10 | 178 | 1.00e-69 | 214 |
| MsG0580024583.01.T01 | MTR_4g098930 | 35.473 | 296 | 138 | 6 | 10 | 297 | 3 | 253 | 2.91e-54 | 177 |
| MsG0580024583.01.T01 | MTR_4g075790 | 36.709 | 237 | 119 | 3 | 61 | 296 | 37 | 243 | 6.24e-50 | 166 |
| MsG0580024583.01.T01 | MTR_4g075790 | 33.668 | 199 | 102 | 2 | 98 | 296 | 9 | 177 | 1.40e-36 | 129 |
| MsG0580024583.01.T01 | MTR_0809s0010 | 32.692 | 208 | 108 | 5 | 10 | 203 | 16 | 205 | 6.76e-25 | 101 |
| MsG0580024583.01.T01 | MTR_2g090265 | 27.686 | 242 | 131 | 8 | 64 | 297 | 52 | 257 | 9.38e-21 | 89.7 |
| MsG0580024583.01.T01 | MTR_0118s0070 | 28.782 | 271 | 137 | 9 | 34 | 296 | 53 | 275 | 1.53e-20 | 89.4 |
| MsG0580024583.01.T01 | MTR_4g028640 | 25.725 | 276 | 150 | 8 | 26 | 296 | 39 | 264 | 2.41e-20 | 88.6 |
| MsG0580024583.01.T01 | MTR_2g090270 | 26.971 | 241 | 132 | 8 | 64 | 296 | 54 | 258 | 2.57e-20 | 88.6 |
| MsG0580024583.01.T01 | MTR_0122s0020 | 27.801 | 241 | 130 | 8 | 64 | 296 | 60 | 264 | 2.14e-17 | 80.5 |
| MsG0580024583.01.T01 | MTR_0122s0020 | 32.468 | 154 | 89 | 6 | 64 | 208 | 60 | 207 | 6.26e-17 | 78.2 |
| MsG0580024583.01.T01 | MTR_4g099380 | 47.368 | 76 | 39 | 1 | 129 | 203 | 1 | 76 | 1.67e-14 | 72.8 |
| MsG0580024583.01.T01 | MTR_4g124340 | 26.337 | 243 | 128 | 10 | 61 | 290 | 75 | 279 | 1.63e-13 | 69.7 |
| MsG0580024583.01.T01 | MTR_4g102450 | 23.361 | 244 | 141 | 7 | 64 | 297 | 55 | 262 | 2.38e-13 | 68.9 |
| MsG0580024583.01.T01 | MTR_5g011210 | 23.868 | 243 | 141 | 7 | 64 | 297 | 56 | 263 | 3.25e-13 | 68.6 |
| MsG0580024583.01.T01 | MTR_1g085460 | 24.797 | 246 | 135 | 10 | 64 | 296 | 49 | 257 | 1.33e-12 | 66.6 |
| MsG0580024583.01.T01 | MTR_7g111010 | 22.549 | 306 | 160 | 14 | 12 | 296 | 5 | 254 | 2.23e-12 | 66.2 |
| MsG0580024583.01.T01 | MTR_8g464350 | 23.200 | 250 | 132 | 10 | 63 | 296 | 49 | 254 | 1.65e-11 | 63.5 |
| MsG0580024583.01.T01 | MTR_7g110990 | 22.711 | 273 | 146 | 12 | 49 | 296 | 7 | 239 | 2.94e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024583.01.T01 | AT4G17030 | 52.465 | 284 | 87 | 5 | 12 | 294 | 11 | 247 | 6.18e-102 | 298 |
| MsG0580024583.01.T01 | AT3G45970 | 34.000 | 250 | 129 | 4 | 49 | 296 | 6 | 221 | 6.83e-46 | 155 |
| MsG0580024583.01.T01 | AT3G45970 | 34.199 | 231 | 122 | 3 | 68 | 296 | 46 | 248 | 8.73e-43 | 148 |
| MsG0580024583.01.T01 | AT4G38400 | 35.780 | 218 | 110 | 2 | 80 | 296 | 60 | 248 | 1.38e-41 | 145 |
| MsG0580024583.01.T01 | AT3G45960 | 33.028 | 218 | 116 | 2 | 80 | 296 | 11 | 199 | 1.86e-40 | 140 |
| MsG0580024583.01.T01 | AT3G45960 | 32.468 | 231 | 125 | 3 | 68 | 296 | 46 | 247 | 2.48e-40 | 141 |
| MsG0580024583.01.T01 | AT1G65680 | 27.113 | 284 | 144 | 9 | 24 | 296 | 42 | 273 | 1.57e-24 | 100 |
| MsG0580024583.01.T01 | AT4G28250 | 26.059 | 307 | 163 | 11 | 6 | 297 | 4 | 261 | 2.46e-19 | 85.9 |
| MsG0580024583.01.T01 | AT2G20750 | 26.749 | 243 | 136 | 8 | 64 | 297 | 59 | 268 | 1.52e-17 | 81.3 |
| MsG0580024583.01.T01 | AT2G20750 | 31.875 | 160 | 99 | 5 | 64 | 215 | 59 | 216 | 1.85e-17 | 79.7 |
| MsG0580024583.01.T01 | AT3G60570 | 27.869 | 244 | 126 | 10 | 64 | 296 | 5 | 209 | 2.54e-17 | 79.3 |
| MsG0580024583.01.T01 | AT4G28250 | 27.632 | 228 | 127 | 8 | 6 | 219 | 4 | 207 | 3.74e-15 | 73.2 |
| MsG0580024583.01.T01 | AT2G45110 | 33.793 | 145 | 87 | 4 | 64 | 201 | 52 | 194 | 1.01e-14 | 72.8 |
| MsG0580024583.01.T01 | AT4G28250 | 25.081 | 307 | 160 | 12 | 6 | 297 | 4 | 255 | 1.22e-14 | 72.8 |
| MsG0580024583.01.T01 | AT5G39310 | 33.103 | 145 | 87 | 5 | 63 | 201 | 108 | 248 | 1.73e-14 | 72.8 |
| MsG0580024583.01.T01 | AT2G40610 | 25.084 | 299 | 164 | 11 | 1 | 290 | 1 | 248 | 2.71e-14 | 71.6 |
| MsG0580024583.01.T01 | AT5G05290 | 25.424 | 236 | 133 | 8 | 64 | 291 | 51 | 251 | 3.28e-14 | 71.2 |
| MsG0580024583.01.T01 | AT4G28250 | 28.302 | 212 | 114 | 8 | 6 | 203 | 4 | 191 | 1.14e-12 | 66.2 |
| MsG0580024583.01.T01 | AT5G39280 | 24.727 | 275 | 149 | 11 | 28 | 296 | 53 | 275 | 1.42e-11 | 63.9 |
| MsG0580024583.01.T01 | AT5G39300 | 24.727 | 275 | 149 | 11 | 28 | 296 | 54 | 276 | 2.41e-11 | 63.5 |
| MsG0580024583.01.T01 | AT5G56320 | 25.871 | 201 | 117 | 7 | 13 | 201 | 9 | 189 | 3.48e-11 | 62.8 |
| MsG0580024583.01.T01 | AT5G56320 | 25.871 | 201 | 117 | 7 | 13 | 201 | 9 | 189 | 3.48e-11 | 62.8 |
| MsG0580024583.01.T01 | AT5G56320 | 25.871 | 201 | 117 | 7 | 13 | 201 | 9 | 189 | 4.04e-11 | 63.2 |
Find 73 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAATTTGGTGCTTAAAATT+AGG | 0.152890 | 5:+6587772 | None:intergenic |
| TCCCCGTGGCTATTCAAATT+TGG | 0.158342 | 5:-6586870 | MsG0580024583.01.T01:CDS |
| TGGCTATAATATCTTGTTTA+AGG | 0.174105 | 5:-6586765 | MsG0580024583.01.T01:CDS |
| TGCTCTAGAGCAACCTATTA+TGG | 0.246885 | 5:-6587691 | MsG0580024583.01.T01:CDS |
| CTTGGATTTGCATAACAATC+TGG | 0.272291 | 5:+6587664 | None:intergenic |
| TACATTTCTTGAAATTGAAT+TGG | 0.284610 | 5:-6586612 | MsG0580024583.01.T01:CDS |
| ACAATCTGGGCTACCATAAT+AGG | 0.288543 | 5:+6587678 | None:intergenic |
| TGAATTGGGTTTACTTGAAA+TGG | 0.289685 | 5:-6586597 | MsG0580024583.01.T01:CDS |
| GCTTGTGGCTATGGTGATTA+TGG | 0.309081 | 5:-6587257 | MsG0580024583.01.T01:CDS |
| TACGCATTGGCCTCCATTCT+TGG | 0.310288 | 5:+6586555 | None:intergenic |
| ATCTCTAGTGATTGGGAAGC+TGG | 0.319429 | 5:-6586422 | MsG0580024583.01.T01:CDS |
| TGACACAAACAAAACCAATT+TGG | 0.321541 | 5:+6587758 | None:intergenic |
| GTGGTAGTGCTGGGCTGTAT+TGG | 0.321854 | 5:-6586463 | MsG0580024583.01.T01:CDS |
| CCATTCTCGTCACACAATTC+TGG | 0.326522 | 5:+6586950 | None:intergenic |
| TGGTAGTGCTGGGCTGTATT+GGG | 0.329472 | 5:-6586462 | MsG0580024583.01.T01:CDS |
| CCCCGTGGCTATTCAAATTT+GGG | 0.333249 | 5:-6586869 | MsG0580024583.01.T01:CDS |
| AGGCCAATGCGTAGGGCATT+TGG | 0.356145 | 5:-6586545 | MsG0580024583.01.T01:CDS |
| TATTAACAATCTCAAAATAA+AGG | 0.362987 | 5:+6586645 | None:intergenic |
| CCGATTTCGGTGTGGGAGAT+AGG | 0.376563 | 5:-6586910 | MsG0580024583.01.T01:CDS |
| CCTATCTCCCACACCGAAAT+CGG | 0.384864 | 5:+6586910 | None:intergenic |
| ACTTGAAATGGTAATGGAAC+AGG | 0.398370 | 5:-6586585 | MsG0580024583.01.T01:CDS |
| AGACTGTTATCTCTAGTGAT+TGG | 0.400920 | 5:-6586430 | MsG0580024583.01.T01:CDS |
| GCCATTCTTATTCTCTATGT+TGG | 0.402224 | 5:-6586710 | MsG0580024583.01.T01:CDS |
| CTCCAAGTGAGTGGTAGTGC+TGG | 0.409157 | 5:-6586473 | MsG0580024583.01.T01:CDS |
| ACATTTCTTGAAATTGAATT+GGG | 0.414085 | 5:-6586611 | MsG0580024583.01.T01:CDS |
| GGGTTTACTTGAAATGGTAA+TGG | 0.423057 | 5:-6586591 | MsG0580024583.01.T01:CDS |
| TTAGAGGGAGCTTGTGGCTA+TGG | 0.433052 | 5:-6587266 | MsG0580024583.01.T01:intron |
| GACTGTTATCTCTAGTGATT+GGG | 0.436426 | 5:-6586429 | MsG0580024583.01.T01:CDS |
| AACTTGATCTCACCTCTTGG+AGG | 0.448456 | 5:+6586500 | None:intergenic |
| AACAGGAAGATTGCCAAGAA+TGG | 0.466268 | 5:-6586568 | MsG0580024583.01.T01:CDS |
| ATTCTTATTCTCTATGTTGG+TGG | 0.472042 | 5:-6586707 | MsG0580024583.01.T01:CDS |
| TCTCTAGTGATTGGGAAGCT+GGG | 0.472781 | 5:-6586421 | MsG0580024583.01.T01:CDS |
| ATTAGTAGAAGACACTTACT+TGG | 0.476087 | 5:+6587646 | None:intergenic |
| TATGTGGTGGTGACCGATTT+CGG | 0.479504 | 5:-6586923 | MsG0580024583.01.T01:CDS |
| TCGCAGGCGTTTCTTCGCTA+TGG | 0.490569 | 5:-6587211 | MsG0580024583.01.T01:CDS |
| TCGCTATGGAAGAATGGATC+TGG | 0.490619 | 5:-6587197 | MsG0580024583.01.T01:CDS |
| CTCAACTTGATCTCACCTCT+TGG | 0.495730 | 5:+6586497 | None:intergenic |
| TTGGATTTGCATAACAATCT+GGG | 0.496289 | 5:+6587665 | None:intergenic |
| CCAGAATTGTGTGACGAGAA+TGG | 0.501363 | 5:-6586950 | MsG0580024583.01.T01:CDS |
| ATCACTAGAGATAACAGTCT+TGG | 0.514327 | 5:+6586433 | None:intergenic |
| CTCTAGTGATTGGGAAGCTG+GGG | 0.520412 | 5:-6586420 | MsG0580024583.01.T01:CDS |
| TTATAGCCATTGTATTTGCA+AGG | 0.532628 | 5:+6586779 | None:intergenic |
| GCCCAGCACTACCACTCACT+TGG | 0.534848 | 5:+6586471 | None:intergenic |
| ACTTGATCTCACCTCTTGGA+GGG | 0.535508 | 5:+6586501 | None:intergenic |
| ACCAACATAGAGAATAAGAA+TGG | 0.537864 | 5:+6586709 | None:intergenic |
| GCCCAAATTTGAATAGCCAC+GGG | 0.544992 | 5:+6586868 | None:intergenic |
| TGCCCAAATTTGAATAGCCA+CGG | 0.546821 | 5:+6586867 | None:intergenic |
| GTTCCAAATGCCCTACGCAT+TGG | 0.550110 | 5:+6586542 | None:intergenic |
| GTAAATGATGGCAATGTCGC+AGG | 0.552094 | 5:-6587227 | MsG0580024583.01.T01:CDS |
| TCCAAGTGAGTGGTAGTGCT+GGG | 0.552324 | 5:-6586472 | MsG0580024583.01.T01:CDS |
| GTTTCTTCGCTATGGAAGAA+TGG | 0.552967 | 5:-6587203 | MsG0580024583.01.T01:CDS |
| AAGAATGGAGGCCAATGCGT+AGG | 0.554025 | 5:-6586553 | MsG0580024583.01.T01:CDS |
| TGGAAGAATGGATCTGGTTG+TGG | 0.555017 | 5:-6587191 | MsG0580024583.01.T01:CDS |
| TCTTGAGAGGAACACAAGAC+AGG | 0.557963 | 5:+6587724 | None:intergenic |
| TATAGCCATTGTATTTGCAA+GGG | 0.561411 | 5:+6586780 | None:intergenic |
| GTGGTGACCGATTTCGGTGT+GGG | 0.566642 | 5:-6586917 | MsG0580024583.01.T01:CDS |
| AACTGCTGTTCAGTTGTGGC+AGG | 0.569473 | 5:-6586672 | MsG0580024583.01.T01:CDS |
| ATAAGAATGGCTAAGTAGTG+AGG | 0.575706 | 5:+6586722 | None:intergenic |
| AGAATGGAGGCCAATGCGTA+GGG | 0.588746 | 5:-6586552 | MsG0580024583.01.T01:CDS |
| TATGGACAGACAGTAAATGA+TGG | 0.603311 | 5:-6587239 | MsG0580024583.01.T01:CDS |
| TAGTGCTGGGCTGTATTGGG+TGG | 0.610771 | 5:-6586459 | MsG0580024583.01.T01:CDS |
| AGGAAGATTGCCAAGAATGG+AGG | 0.614849 | 5:-6586565 | MsG0580024583.01.T01:CDS |
| TGACGAGAATGGAGCATATG+TGG | 0.619740 | 5:-6586939 | MsG0580024583.01.T01:CDS |
| AGAATCCCTTGCAAATACAA+TGG | 0.620915 | 5:-6586785 | MsG0580024583.01.T01:CDS |
| GCAAACAAAAGAGTCTTGAG+AGG | 0.624968 | 5:+6587711 | None:intergenic |
| ATGTAACTGCTGTTCAGTTG+TGG | 0.651186 | 5:-6586676 | MsG0580024583.01.T01:CDS |
| GGTGGTGACCGATTTCGGTG+TGG | 0.653687 | 5:-6586918 | MsG0580024583.01.T01:CDS |
| AAGTTGAGGCTCCAAGTGAG+TGG | 0.655665 | 5:-6586482 | MsG0580024583.01.T01:CDS |
| GACTTCATCATGAGTCCCCG+TGG | 0.688519 | 5:-6586884 | MsG0580024583.01.T01:CDS |
| GATGCAGAGAACCCTCCAAG+AGG | 0.690257 | 5:-6586512 | MsG0580024583.01.T01:CDS |
| CCCAAATTTGAATAGCCACG+GGG | 0.693150 | 5:+6586869 | None:intergenic |
| CAAGAGGTGAGATCAAGTTG+AGG | 0.697595 | 5:-6586496 | MsG0580024583.01.T01:CDS |
| CGAGAATGGAGCATATGTGG+TGG | 0.757236 | 5:-6586936 | MsG0580024583.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAATAAATAATGATGAAAT+AGG | - | Chr5:6587182-6587201 | MsG0580024583.01.T01:CDS | 10.0% |
| !! | TGAAGTAATATATATTATAA+AGG | - | Chr5:6587115-6587134 | MsG0580024583.01.T01:intron | 10.0% |
| !! | TATTAACAATCTCAAAATAA+AGG | + | Chr5:6587527-6587546 | None:intergenic | 15.0% |
| !!! | ATTCAATTTTTATCTCAAAT+TGG | - | Chr5:6586681-6586700 | MsG0580024583.01.T01:CDS | 15.0% |
| !! | ACACATTTATAAAAATTGAC+AGG | + | Chr5:6586721-6586740 | None:intergenic | 20.0% |
| !! | ACATTTCTTGAAATTGAATT+GGG | - | Chr5:6587558-6587577 | MsG0580024583.01.T01:intron | 20.0% |
| !! | CACATTTATAAAAATTGACA+GGG | + | Chr5:6586720-6586739 | None:intergenic | 20.0% |
| !! | TACATTTCTTGAAATTGAAT+TGG | - | Chr5:6587557-6587576 | MsG0580024583.01.T01:intron | 20.0% |
| ! | AAATAATGATGAAATAGGTG+AGG | - | Chr5:6587187-6587206 | MsG0580024583.01.T01:CDS | 25.0% |
| ! | TATTCAAATTTGGGCAAAAA+TGG | - | Chr5:6587309-6587328 | MsG0580024583.01.T01:intron | 25.0% |
| !! | AACTAATTGTTAGTTGATGT+TGG | + | Chr5:6587151-6587170 | None:intergenic | 25.0% |
| !! | AGAACTTTTTAAATATGGTG+TGG | - | Chr5:6587344-6587363 | MsG0580024583.01.T01:intron | 25.0% |
| !! | CATTGAATCGGTTTATAAAT+CGG | + | Chr5:6586788-6586807 | None:intergenic | 25.0% |
| !! | TCTGCAGAACTTTTTAAATA+TGG | - | Chr5:6587339-6587358 | MsG0580024583.01.T01:intron | 25.0% |
| !! | TGGCTATAATATCTTGTTTA+AGG | - | Chr5:6587404-6587423 | MsG0580024583.01.T01:intron | 25.0% |
| !!! | CAGTATACAACATTTTTTTC+CGG | - | Chr5:6586811-6586830 | MsG0580024583.01.T01:CDS | 25.0% |
| ACCAACATAGAGAATAAGAA+TGG | + | Chr5:6587463-6587482 | None:intergenic | 30.0% | |
| ATTAGTAGAAGACACTTACT+TGG | + | Chr5:6586526-6586545 | None:intergenic | 30.0% | |
| ATTCTTATTCTCTATGTTGG+TGG | - | Chr5:6587462-6587481 | MsG0580024583.01.T01:intron | 30.0% | |
| TATAGCCATTGTATTTGCAA+GGG | + | Chr5:6587392-6587411 | None:intergenic | 30.0% | |
| TGAATTGGGTTTACTTGAAA+TGG | - | Chr5:6587572-6587591 | MsG0580024583.01.T01:intron | 30.0% | |
| TGACACAAACAAAACCAATT+TGG | + | Chr5:6586414-6586433 | None:intergenic | 30.0% | |
| TGTATACTGTCTCATTGAAT+CGG | + | Chr5:6586800-6586819 | None:intergenic | 30.0% | |
| TTATAGCCATTGTATTTGCA+AGG | + | Chr5:6587393-6587412 | None:intergenic | 30.0% | |
| TTGGATTTGCATAACAATCT+GGG | + | Chr5:6586507-6586526 | None:intergenic | 30.0% | |
| ! | ACTTTTTAAATATGGTGTGG+TGG | - | Chr5:6587347-6587366 | MsG0580024583.01.T01:intron | 30.0% |
| ! | CCTAATTTTAAGCACCAAAT+TGG | - | Chr5:6586397-6586416 | MsG0580024583.01.T01:CDS | 30.0% |
| !! | AGCTATTAACCTGTTTTAGA+GGG | - | Chr5:6586888-6586907 | MsG0580024583.01.T01:CDS | 30.0% |
| !! | CCAATTTGGTGCTTAAAATT+AGG | + | Chr5:6586400-6586419 | None:intergenic | 30.0% |
| !! | TAGCTATTAACCTGTTTTAG+AGG | - | Chr5:6586887-6586906 | MsG0580024583.01.T01:CDS | 30.0% |
| AAAAATTGACAGGGTTGAGT+AGG | + | Chr5:6586711-6586730 | None:intergenic | 35.0% | |
| ACCTAGACATTGCATTACTT+TGG | - | Chr5:6586837-6586856 | MsG0580024583.01.T01:CDS | 35.0% | |
| AGAATCCCTTGCAAATACAA+TGG | - | Chr5:6587384-6587403 | MsG0580024583.01.T01:intron | 35.0% | |
| AGACTGTTATCTCTAGTGAT+TGG | - | Chr5:6587739-6587758 | MsG0580024583.01.T01:CDS | 35.0% | |
| ATAAGAATGGCTAAGTAGTG+AGG | + | Chr5:6587450-6587469 | None:intergenic | 35.0% | |
| ATCACTAGAGATAACAGTCT+TGG | + | Chr5:6587739-6587758 | None:intergenic | 35.0% | |
| CTTGGATTTGCATAACAATC+TGG | + | Chr5:6586508-6586527 | None:intergenic | 35.0% | |
| GACTGTTATCTCTAGTGATT+GGG | - | Chr5:6587740-6587759 | MsG0580024583.01.T01:CDS | 35.0% | |
| GCCATTCTTATTCTCTATGT+TGG | - | Chr5:6587459-6587478 | MsG0580024583.01.T01:intron | 35.0% | |
| GGGTTTACTTGAAATGGTAA+TGG | - | Chr5:6587578-6587597 | MsG0580024583.01.T01:intron | 35.0% | |
| TATGGACAGACAGTAAATGA+TGG | - | Chr5:6586930-6586949 | MsG0580024583.01.T01:CDS | 35.0% | |
| !! | ACTTGAAATGGTAATGGAAC+AGG | - | Chr5:6587584-6587603 | MsG0580024583.01.T01:intron | 35.0% |
| !!! | GAGTGTTGTGCACATTTTTT+AGG | - | Chr5:6586744-6586763 | MsG0580024583.01.T01:CDS | 35.0% |
| AACAGGAAGATTGCCAAGAA+TGG | - | Chr5:6587601-6587620 | MsG0580024583.01.T01:intron | 40.0% | |
| AATGCAATGTCTAGGTTCAC+CGG | + | Chr5:6586833-6586852 | None:intergenic | 40.0% | |
| ACAATCTGGGCTACCATAAT+AGG | + | Chr5:6586494-6586513 | None:intergenic | 40.0% | |
| ATGTAACTGCTGTTCAGTTG+TGG | - | Chr5:6587493-6587512 | MsG0580024583.01.T01:intron | 40.0% | |
| GCAAACAAAAGAGTCTTGAG+AGG | + | Chr5:6586461-6586480 | None:intergenic | 40.0% | |
| GCCAAAGTAATGCAATGTCT+AGG | + | Chr5:6586841-6586860 | None:intergenic | 40.0% | |
| GTTTCTTCGCTATGGAAGAA+TGG | - | Chr5:6586966-6586985 | MsG0580024583.01.T01:CDS | 40.0% | |
| TGCCCAAATTTGAATAGCCA+CGG | + | Chr5:6587305-6587324 | None:intergenic | 40.0% | |
| !! | TGCTCTAGAGCAACCTATTA+TGG | - | Chr5:6586478-6586497 | MsG0580024583.01.T01:CDS | 40.0% |
| AACTTGATCTCACCTCTTGG+AGG | + | Chr5:6587672-6587691 | None:intergenic | 45.0% | |
| ACTTGATCTCACCTCTTGGA+GGG | + | Chr5:6587671-6587690 | None:intergenic | 45.0% | |
| AGGAAGATTGCCAAGAATGG+AGG | - | Chr5:6587604-6587623 | MsG0580024583.01.T01:intron | 45.0% | |
| ATCTCTAGTGATTGGGAAGC+TGG | - | Chr5:6587747-6587766 | MsG0580024583.01.T01:CDS | 45.0% | |
| CCAGAATTGTGTGACGAGAA+TGG | - | Chr5:6587219-6587238 | MsG0580024583.01.T01:CDS | 45.0% | |
| CCATTCTCGTCACACAATTC+TGG | + | Chr5:6587222-6587241 | None:intergenic | 45.0% | |
| CCCAAATTTGAATAGCCACG+GGG | + | Chr5:6587303-6587322 | None:intergenic | 45.0% | |
| CCCCGTGGCTATTCAAATTT+GGG | - | Chr5:6587300-6587319 | MsG0580024583.01.T01:intron | 45.0% | |
| CTCAACTTGATCTCACCTCT+TGG | + | Chr5:6587675-6587694 | None:intergenic | 45.0% | |
| GCCCAAATTTGAATAGCCAC+GGG | + | Chr5:6587304-6587323 | None:intergenic | 45.0% | |
| GCTTGTGGCTATGGTGATTA+TGG | - | Chr5:6586912-6586931 | MsG0580024583.01.T01:CDS | 45.0% | |
| GTAAATGATGGCAATGTCGC+AGG | - | Chr5:6586942-6586961 | MsG0580024583.01.T01:CDS | 45.0% | |
| TCCCCGTGGCTATTCAAATT+TGG | - | Chr5:6587299-6587318 | MsG0580024583.01.T01:intron | 45.0% | |
| TCGCTATGGAAGAATGGATC+TGG | - | Chr5:6586972-6586991 | MsG0580024583.01.T01:intron | 45.0% | |
| TCTCTAGTGATTGGGAAGCT+GGG | - | Chr5:6587748-6587767 | MsG0580024583.01.T01:CDS | 45.0% | |
| TCTTGAGAGGAACACAAGAC+AGG | + | Chr5:6586448-6586467 | None:intergenic | 45.0% | |
| TGACGAGAATGGAGCATATG+TGG | - | Chr5:6587230-6587249 | MsG0580024583.01.T01:CDS | 45.0% | |
| TGGAAGAATGGATCTGGTTG+TGG | - | Chr5:6586978-6586997 | MsG0580024583.01.T01:intron | 45.0% | |
| ! | CAAGAGGTGAGATCAAGTTG+AGG | - | Chr5:6587673-6587692 | MsG0580024583.01.T01:CDS | 45.0% |
| ! | GAGGATTTTTGCTGCGTTCA+TGG | + | Chr5:6587431-6587450 | None:intergenic | 45.0% |
| ! | TATGTGGTGGTGACCGATTT+CGG | - | Chr5:6587246-6587265 | MsG0580024583.01.T01:CDS | 45.0% |
| AACTGCTGTTCAGTTGTGGC+AGG | - | Chr5:6587497-6587516 | MsG0580024583.01.T01:intron | 50.0% | |
| AAGAATGGAGGCCAATGCGT+AGG | - | Chr5:6587616-6587635 | MsG0580024583.01.T01:intron | 50.0% | |
| AAGTTGAGGCTCCAAGTGAG+TGG | - | Chr5:6587687-6587706 | MsG0580024583.01.T01:CDS | 50.0% | |
| AGAATGGAGGCCAATGCGTA+GGG | - | Chr5:6587617-6587636 | MsG0580024583.01.T01:CDS | 50.0% | |
| CCTATCTCCCACACCGAAAT+CGG | + | Chr5:6587262-6587281 | None:intergenic | 50.0% | |
| CGAGAATGGAGCATATGTGG+TGG | - | Chr5:6587233-6587252 | MsG0580024583.01.T01:CDS | 50.0% | |
| CTCTAGTGATTGGGAAGCTG+GGG | - | Chr5:6587749-6587768 | MsG0580024583.01.T01:CDS | 50.0% | |
| GTTCCAAATGCCCTACGCAT+TGG | + | Chr5:6587630-6587649 | None:intergenic | 50.0% | |
| TTAGAGGGAGCTTGTGGCTA+TGG | - | Chr5:6586903-6586922 | MsG0580024583.01.T01:CDS | 50.0% | |
| ! | TACGCATTGGCCTCCATTCT+TGG | + | Chr5:6587617-6587636 | None:intergenic | 50.0% |
| ! | TCCAAGTGAGTGGTAGTGCT+GGG | - | Chr5:6587697-6587716 | MsG0580024583.01.T01:CDS | 50.0% |
| ! | TGGTAGTGCTGGGCTGTATT+GGG | - | Chr5:6587707-6587726 | MsG0580024583.01.T01:CDS | 50.0% |
| !! | CCACAAGCTCCCTCTAAAAC+AGG | + | Chr5:6586900-6586919 | None:intergenic | 50.0% |
| !!! | CCTGTTTTAGAGGGAGCTTG+TGG | - | Chr5:6586897-6586916 | MsG0580024583.01.T01:CDS | 50.0% |
| GACTTCATCATGAGTCCCCG+TGG | - | Chr5:6587285-6587304 | MsG0580024583.01.T01:intron | 55.0% | |
| GATGCAGAGAACCCTCCAAG+AGG | - | Chr5:6587657-6587676 | MsG0580024583.01.T01:CDS | 55.0% | |
| TCGCAGGCGTTTCTTCGCTA+TGG | - | Chr5:6586958-6586977 | MsG0580024583.01.T01:CDS | 55.0% | |
| ! | AGGCCAATGCGTAGGGCATT+TGG | - | Chr5:6587624-6587643 | MsG0580024583.01.T01:CDS | 55.0% |
| ! | CCGATTTCGGTGTGGGAGAT+AGG | - | Chr5:6587259-6587278 | MsG0580024583.01.T01:CDS | 55.0% |
| ! | CTCCAAGTGAGTGGTAGTGC+TGG | - | Chr5:6587696-6587715 | MsG0580024583.01.T01:CDS | 55.0% |
| ! | GTGGTAGTGCTGGGCTGTAT+TGG | - | Chr5:6587706-6587725 | MsG0580024583.01.T01:CDS | 55.0% |
| ! | GTGGTGACCGATTTCGGTGT+GGG | - | Chr5:6587252-6587271 | MsG0580024583.01.T01:CDS | 55.0% |
| ! | TAGTGCTGGGCTGTATTGGG+TGG | - | Chr5:6587710-6587729 | MsG0580024583.01.T01:CDS | 55.0% |
| GCCCAGCACTACCACTCACT+TGG | + | Chr5:6587701-6587720 | None:intergenic | 60.0% | |
| ! | GGTGGTGACCGATTTCGGTG+TGG | - | Chr5:6587251-6587270 | MsG0580024583.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 6586388 | 6587803 | 6586388 | ID=MsG0580024583.01;Name=MsG0580024583.01 |
| Chr5 | mRNA | 6586388 | 6587803 | 6586388 | ID=MsG0580024583.01.T01;Parent=MsG0580024583.01;Name=MsG0580024583.01.T01;_AED=0.24;_eAED=0.26;_QI=0|0.5|0.33|1|0|0.33|3|0|297 |
| Chr5 | exon | 6587617 | 6587803 | 6587617 | ID=MsG0580024583.01.T01:exon:2530;Parent=MsG0580024583.01.T01 |
| Chr5 | exon | 6587178 | 6587284 | 6587178 | ID=MsG0580024583.01.T01:exon:2529;Parent=MsG0580024583.01.T01 |
| Chr5 | exon | 6586388 | 6586987 | 6586388 | ID=MsG0580024583.01.T01:exon:2528;Parent=MsG0580024583.01.T01 |
| Chr5 | CDS | 6587617 | 6587803 | 6587617 | ID=MsG0580024583.01.T01:cds;Parent=MsG0580024583.01.T01 |
| Chr5 | CDS | 6587178 | 6587284 | 6587178 | ID=MsG0580024583.01.T01:cds;Parent=MsG0580024583.01.T01 |
| Chr5 | CDS | 6586388 | 6586987 | 6586388 | ID=MsG0580024583.01.T01:cds;Parent=MsG0580024583.01.T01 |
| Gene Sequence |
| Protein sequence |
