AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280008970.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008970.01.T01 MTR_2g450210 89.785 186 9 2 1 186 830 1005 8.70e-103 320
MsG0280008970.01.T01 MTR_2g450210 89.785 186 9 2 1 186 830 1005 3.34e-102 321
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008970.01.T01 AT5G58040 37.079 178 80 2 3 171 716 870 6.73e-19 84.7
MsG0280008970.01.T01 AT5G58040 37.079 178 80 2 3 171 716 870 7.06e-19 84.7

Find 64 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCATCATCTTTCTGCCTAT+TGG 0.245832 2:-42682112 None:intergenic
AGAGAGGAAGGAAGGAGTTC+TGG 0.259470 2:+42682227 MsG0280008970.01.T01:CDS
TTAGCCCTATCCCTCTTTCT+AGG 0.260995 2:-42681742 None:intergenic
CGTCCTTTCTCCTCTTGATA+TGG 0.283488 2:-42681933 None:intergenic
GAAAGGACGAGGAGTATCTT+AGG 0.317908 2:+42681947 MsG0280008970.01.T01:CDS
CTATTGGTGTATTGATCATC+AGG 0.362396 2:-42682096 None:intergenic
GGGGCGATAGGCAAAGAGAT+AGG 0.369351 2:+42682151 MsG0280008970.01.T01:CDS
CTCTCTTTCACGCTTAGGTA+GGG 0.388519 2:-42682210 None:intergenic
GAGATGAGGGCGTGAGGGTT+AGG 0.399629 2:+42681890 MsG0280008970.01.T01:CDS
CCTTCCTCTCTTTCACGCTT+AGG 0.410977 2:-42682215 None:intergenic
CTGATGATCAATACACCAAT+AGG 0.417569 2:+42682097 MsG0280008970.01.T01:CDS
CCTCTCTTTCACGCTTAGGT+AGG 0.432633 2:-42682211 None:intergenic
TCTAGAAAGCAGTTGGATAA+TGG 0.436918 2:+42681808 MsG0280008970.01.T01:CDS
ATTCCTTATGAAAAGGATGT+TGG 0.444364 2:+42681841 MsG0280008970.01.T01:CDS
GTATCTTAGGAGGGAACATA+TGG 0.456696 2:+42681960 MsG0280008970.01.T01:CDS
ATGAACCTAGAAAGAGGGAT+AGG 0.457731 2:+42681737 MsG0280008970.01.T01:CDS
TTCCTTATGAAAAGGATGTT+GGG 0.467414 2:+42681842 MsG0280008970.01.T01:CDS
GGGACAGTTCTGACATGGAT+TGG 0.471287 2:+42681671 None:intergenic
CTACAGGATTCCTTATGAAA+AGG 0.478323 2:+42681834 MsG0280008970.01.T01:CDS
CTTTCACTCTAGAAAGCAGT+TGG 0.489381 2:+42681801 MsG0280008970.01.T01:CDS
GCCCCTCTCCTGCATTAGCC+AGG 0.492727 2:-42682133 None:intergenic
GGACAGTTCTGACATGGATT+GGG 0.498840 2:+42681672 None:intergenic
GGGCGATAGGCAAAGAGATA+GGG 0.512814 2:+42682152 MsG0280008970.01.T01:CDS
TCTCTTTCACGCTTAGGTAG+GGG 0.513727 2:-42682209 None:intergenic
ACGAGAAAGAGATGAAGTAT+TGG 0.528916 2:+42682032 MsG0280008970.01.T01:CDS
GAACTAATGAACCTAGAAAG+AGG 0.530482 2:+42681731 MsG0280008970.01.T01:CDS
AGTTGGATAATGGTAGCTAC+AGG 0.535479 2:+42681818 MsG0280008970.01.T01:CDS
AGATGATGCCTGGCTAATGC+AGG 0.538158 2:+42682125 MsG0280008970.01.T01:CDS
ATAGGCAGAAAGATGATGCC+TGG 0.540728 2:+42682115 MsG0280008970.01.T01:CDS
TGAACCTAGAAAGAGGGATA+GGG 0.543735 2:+42681738 MsG0280008970.01.T01:CDS
TGCCTGGCTAATGCAGGAGA+GGG 0.551125 2:+42682131 MsG0280008970.01.T01:CDS
AGGACGAGGAGTATCTTAGG+AGG 0.553205 2:+42681950 MsG0280008970.01.T01:CDS
TCGGTTGAAACTGTCCATGA+TGG 0.570881 2:+42682185 MsG0280008970.01.T01:CDS
CCTAAGCGTGAAAGAGAGGA+AGG 0.574352 2:+42682215 MsG0280008970.01.T01:CDS
AGCGTGAAAGAGAGGAAGGA+AGG 0.578147 2:+42682219 MsG0280008970.01.T01:CDS
GAGATAGGGAGGAGTGGCAT+CGG 0.578382 2:+42682166 MsG0280008970.01.T01:CDS
GAGGGTTAGGTACGAAGCTG+TGG 0.581406 2:+42681903 MsG0280008970.01.T01:CDS
AACTAATGAACCTAGAAAGA+GGG 0.582955 2:+42681732 MsG0280008970.01.T01:CDS
GGGAAAGAGATGAGGGCGTG+AGG 0.583983 2:+42681884 MsG0280008970.01.T01:CDS
GCTTAGGTAGGGGTCCATCA+TGG 0.585286 2:-42682199 None:intergenic
TAATGCAGGAGAGGGGCGAT+AGG 0.589485 2:+42682139 MsG0280008970.01.T01:CDS
CGATAGGCAAAGAGATAGGG+AGG 0.594330 2:+42682155 MsG0280008970.01.T01:CDS
TTACCATATCAAGAGGAGAA+AGG 0.596554 2:+42681930 MsG0280008970.01.T01:CDS
TATCAAGAGGAGAAAGGACG+AGG 0.599347 2:+42681936 MsG0280008970.01.T01:CDS
GGAAAGAGATGAGGGCGTGA+GGG 0.599691 2:+42681885 MsG0280008970.01.T01:CDS
CCTACCTAAGCGTGAAAGAG+AGG 0.603911 2:+42682211 MsG0280008970.01.T01:CDS
GGCAAAGAGATAGGGAGGAG+TGG 0.608356 2:+42682160 MsG0280008970.01.T01:CDS
ATGCCTGGCTAATGCAGGAG+AGG 0.618822 2:+42682130 MsG0280008970.01.T01:CDS
AGACACAGGGAAAGAGATGA+GGG 0.621392 2:+42681877 MsG0280008970.01.T01:CDS
GTTCTGACATGGATTGGGCA+AGG 0.623646 2:+42681677 None:intergenic
GCCTGGCTAATGCAGGAGAG+GGG 0.624556 2:+42682132 MsG0280008970.01.T01:CDS
AAGACACAGGGAAAGAGATG+AGG 0.626916 2:+42681876 MsG0280008970.01.T01:CDS
GGTTAGGTACGAAGCTGTGG+AGG 0.632492 2:+42681906 MsG0280008970.01.T01:CDS
TGGAGGATTACCATATCAAG+AGG 0.635598 2:+42681923 MsG0280008970.01.T01:CDS
GTTCATCTTTAGCACTAACA+CGG 0.644200 2:-42682288 None:intergenic
GGTCAAGAGACTCAAGACAC+AGG 0.647038 2:+42681863 MsG0280008970.01.T01:CDS
TTCTGGAAGAAGTGTACGAG+GGG 0.649843 2:+42682244 MsG0280008970.01.T01:CDS
GGACGAGGAGTATCTTAGGA+GGG 0.661767 2:+42681951 MsG0280008970.01.T01:CDS
GTCAAGAGACTCAAGACACA+GGG 0.666678 2:+42681864 MsG0280008970.01.T01:CDS
GCTACAGAGAAAATGCTAGT+CGG 0.676581 2:+42682004 MsG0280008970.01.T01:CDS
AGTTCTGGAAGAAGTGTACG+AGG 0.694164 2:+42682242 MsG0280008970.01.T01:CDS
TATTGGTGTATTGATCATCA+GGG 0.694908 2:-42682095 None:intergenic
GTTCTGGAAGAAGTGTACGA+GGG 0.696011 2:+42682243 MsG0280008970.01.T01:CDS
GACAAAGAAGATATTCCACA+TGG 0.711263 2:+42681982 MsG0280008970.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AACTAATGAACCTAGAAAGA+GGG + Chr2:42681732-42681751 MsG0280008970.01.T01:CDS 30.0%
ATTCCTTATGAAAAGGATGT+TGG + Chr2:42681841-42681860 MsG0280008970.01.T01:CDS 30.0%
TTCCTTATGAAAAGGATGTT+GGG + Chr2:42681842-42681861 MsG0280008970.01.T01:CDS 30.0%
! TATTGGTGTATTGATCATCA+GGG - Chr2:42682098-42682117 None:intergenic 30.0%
ACGAGAAAGAGATGAAGTAT+TGG + Chr2:42682032-42682051 MsG0280008970.01.T01:CDS 35.0%
CTACAGGATTCCTTATGAAA+AGG + Chr2:42681834-42681853 MsG0280008970.01.T01:CDS 35.0%
CTGATGATCAATACACCAAT+AGG + Chr2:42682097-42682116 MsG0280008970.01.T01:CDS 35.0%
GAACTAATGAACCTAGAAAG+AGG + Chr2:42681731-42681750 MsG0280008970.01.T01:CDS 35.0%
GACAAAGAAGATATTCCACA+TGG + Chr2:42681982-42682001 MsG0280008970.01.T01:CDS 35.0%
TCTAGAAAGCAGTTGGATAA+TGG + Chr2:42681808-42681827 MsG0280008970.01.T01:CDS 35.0%
TTACCATATCAAGAGGAGAA+AGG + Chr2:42681930-42681949 MsG0280008970.01.T01:CDS 35.0%
! CTATTGGTGTATTGATCATC+AGG - Chr2:42682099-42682118 None:intergenic 35.0%
ATGAACCTAGAAAGAGGGAT+AGG + Chr2:42681737-42681756 MsG0280008970.01.T01:CDS 40.0%
GCTACAGAGAAAATGCTAGT+CGG + Chr2:42682004-42682023 MsG0280008970.01.T01:CDS 40.0%
GTATCTTAGGAGGGAACATA+TGG + Chr2:42681960-42681979 MsG0280008970.01.T01:CDS 40.0%
TGAACCTAGAAAGAGGGATA+GGG + Chr2:42681738-42681757 MsG0280008970.01.T01:CDS 40.0%
TGGAGGATTACCATATCAAG+AGG + Chr2:42681923-42681942 MsG0280008970.01.T01:CDS 40.0%
! AGTTGGATAATGGTAGCTAC+AGG + Chr2:42681818-42681837 MsG0280008970.01.T01:CDS 40.0%
! CTTTCACTCTAGAAAGCAGT+TGG + Chr2:42681801-42681820 MsG0280008970.01.T01:CDS 40.0%
! GACCCAACATCCTTTTCATA+AGG - Chr2:42681847-42681866 None:intergenic 40.0%
AAGACACAGGGAAAGAGATG+AGG + Chr2:42681876-42681895 MsG0280008970.01.T01:CDS 45.0%
AGACACAGGGAAAGAGATGA+GGG + Chr2:42681877-42681896 MsG0280008970.01.T01:CDS 45.0%
ATAGGCAGAAAGATGATGCC+TGG + Chr2:42682115-42682134 MsG0280008970.01.T01:CDS 45.0%
CGTCCTTTCTCCTCTTGATA+TGG - Chr2:42681936-42681955 None:intergenic 45.0%
CTCTCTTTCACGCTTAGGTA+GGG - Chr2:42682213-42682232 None:intergenic 45.0%
GGCATCATCTTTCTGCCTAT+TGG - Chr2:42682115-42682134 None:intergenic 45.0%
GTCAAGAGACTCAAGACACA+GGG + Chr2:42681864-42681883 MsG0280008970.01.T01:CDS 45.0%
TATCAAGAGGAGAAAGGACG+AGG + Chr2:42681936-42681955 MsG0280008970.01.T01:CDS 45.0%
TCGGTTGAAACTGTCCATGA+TGG + Chr2:42682185-42682204 MsG0280008970.01.T01:CDS 45.0%
TCTCTTTCACGCTTAGGTAG+GGG - Chr2:42682212-42682231 None:intergenic 45.0%
TTAGCCCTATCCCTCTTTCT+AGG - Chr2:42681745-42681764 None:intergenic 45.0%
! AGGGGCTGAAGAAAAAGCAT+GGG + Chr2:42682262-42682281 MsG0280008970.01.T01:CDS 45.0%
! GCATTTTCTCTGTAGCCATG+TGG - Chr2:42682000-42682019 None:intergenic 45.0%
! TTCTGGAAGAAGTGTACGAG+GGG + Chr2:42682244-42682263 MsG0280008970.01.T01:CDS 45.0%
!! AGTTCTGGAAGAAGTGTACG+AGG + Chr2:42682242-42682261 MsG0280008970.01.T01:CDS 45.0%
!! GAAAGGACGAGGAGTATCTT+AGG + Chr2:42681947-42681966 MsG0280008970.01.T01:CDS 45.0%
!! GTTCTGGAAGAAGTGTACGA+GGG + Chr2:42682243-42682262 MsG0280008970.01.T01:CDS 45.0%
AGAGAGGAAGGAAGGAGTTC+TGG + Chr2:42682227-42682246 MsG0280008970.01.T01:CDS 50.0%
AGATGATGCCTGGCTAATGC+AGG + Chr2:42682125-42682144 MsG0280008970.01.T01:CDS 50.0%
AGCGTGAAAGAGAGGAAGGA+AGG + Chr2:42682219-42682238 MsG0280008970.01.T01:CDS 50.0%
CCTAAGCGTGAAAGAGAGGA+AGG + Chr2:42682215-42682234 MsG0280008970.01.T01:CDS 50.0%
CCTACCTAAGCGTGAAAGAG+AGG + Chr2:42682211-42682230 MsG0280008970.01.T01:CDS 50.0%
CCTCTCTTTCACGCTTAGGT+AGG - Chr2:42682214-42682233 None:intergenic 50.0%
CCTTCCTCTCTTTCACGCTT+AGG - Chr2:42682218-42682237 None:intergenic 50.0%
CGATAGGCAAAGAGATAGGG+AGG + Chr2:42682155-42682174 MsG0280008970.01.T01:CDS 50.0%
GAGGGGCTGAAGAAAAAGCA+TGG + Chr2:42682261-42682280 MsG0280008970.01.T01:CDS 50.0%
GGGCGATAGGCAAAGAGATA+GGG + Chr2:42682152-42682171 MsG0280008970.01.T01:CDS 50.0%
GGTCAAGAGACTCAAGACAC+AGG + Chr2:42681863-42681882 MsG0280008970.01.T01:CDS 50.0%
! GGCTGAAGAAAAAGCATGGG+TGG + Chr2:42682265-42682284 MsG0280008970.01.T01:CDS 50.0%
!! AGGACGAGGAGTATCTTAGG+AGG + Chr2:42681950-42681969 MsG0280008970.01.T01:CDS 50.0%
!! GGACGAGGAGTATCTTAGGA+GGG + Chr2:42681951-42681970 MsG0280008970.01.T01:CDS 50.0%
ATGCCTGGCTAATGCAGGAG+AGG + Chr2:42682130-42682149 MsG0280008970.01.T01:CDS 55.0%
GAGATAGGGAGGAGTGGCAT+CGG + Chr2:42682166-42682185 MsG0280008970.01.T01:CDS 55.0%
GAGGGTTAGGTACGAAGCTG+TGG + Chr2:42681903-42681922 MsG0280008970.01.T01:CDS 55.0%
GCTTAGGTAGGGGTCCATCA+TGG - Chr2:42682202-42682221 None:intergenic 55.0%
GGCAAAGAGATAGGGAGGAG+TGG + Chr2:42682160-42682179 MsG0280008970.01.T01:CDS 55.0%
GGGGCGATAGGCAAAGAGAT+AGG + Chr2:42682151-42682170 MsG0280008970.01.T01:CDS 55.0%
GGTTAGGTACGAAGCTGTGG+AGG + Chr2:42681906-42681925 MsG0280008970.01.T01:CDS 55.0%
TAATGCAGGAGAGGGGCGAT+AGG + Chr2:42682139-42682158 MsG0280008970.01.T01:CDS 55.0%
TGCCTGGCTAATGCAGGAGA+GGG + Chr2:42682131-42682150 MsG0280008970.01.T01:CDS 55.0%
! GGAAAGAGATGAGGGCGTGA+GGG + Chr2:42681885-42681904 MsG0280008970.01.T01:CDS 55.0%
GCCTGGCTAATGCAGGAGAG+GGG + Chr2:42682132-42682151 MsG0280008970.01.T01:CDS 60.0%
! GAGATGAGGGCGTGAGGGTT+AGG + Chr2:42681890-42681909 MsG0280008970.01.T01:CDS 60.0%
! GGGAAAGAGATGAGGGCGTG+AGG + Chr2:42681884-42681903 MsG0280008970.01.T01:CDS 60.0%
GCCCCTCTCCTGCATTAGCC+AGG - Chr2:42682136-42682155 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 42681685 42682296 42681685 ID=MsG0280008970.01;Name=MsG0280008970.01
Chr2 mRNA 42681685 42682296 42681685 ID=MsG0280008970.01.T01;Parent=MsG0280008970.01;Name=MsG0280008970.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|203
Chr2 exon 42681685 42682296 42681685 ID=MsG0280008970.01.T01:exon:27224;Parent=MsG0280008970.01.T01
Chr2 CDS 42681685 42682296 42681685 ID=MsG0280008970.01.T01:cds;Parent=MsG0280008970.01.T01
Gene Sequence

>MsG0280008970.01.T01

ATGGATTGGGCAAGGAGAGATGATGACGTCTATAACAGAAAGATTAGAACTAATGAACCTAGAAAGAGGGATAGGGCTAAGCTTCGAGAAAATGAGAGAAACGACAAAGAAGATAGCTTTCACTCTAGAAAGCAGTTGGATAATGGTAGCTACAGGATTCCTTATGAAAAGGATGTTGGGTCAAGAGACTCAAGACACAGGGAAAGAGATGAGGGCGTGAGGGTTAGGTACGAAGCTGTGGAGGATTACCATATCAAGAGGAGAAAGGACGAGGAGTATCTTAGGAGGGAACATATGGACAAAGAAGATATTCCACATGGCTACAGAGAAAATGCTAGTCGGCGTAGACGAGAAAGAGATGAAGTATTGGATCAACGTAAGAGAGATGAAATGCAAAGAAGTAGAGATTACCCTGATGATCAATACACCAATAGGCAGAAAGATGATGCCTGGCTAATGCAGGAGAGGGGCGATAGGCAAAGAGATAGGGAGGAGTGGCATCGGTTGAAACTGTCCATGATGGACCCCTACCTAAGCGTGAAAGAGAGGAAGGAAGGAGTTCTGGAAGAAGTGTACGAGGGGCTGAAGAAAAAGCATGGGTGGCCGTGTTAG

Protein sequence

>MsG0280008970.01.T01

MDWARRDDDVYNRKIRTNEPRKRDRAKLRENERNDKEDSFHSRKQLDNGSYRIPYEKDVGSRDSRHRERDEGVRVRYEAVEDYHIKRRKDEEYLRREHMDKEDIPHGYRENASRRRRERDEVLDQRKRDEMQRSRDYPDDQYTNRQKDDAWLMQERGDRQRDREEWHRLKLSMMDPYLSVKERKEGVLEEVYEGLKKKHGWPC*