Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0945630 | XP_003630536.2 | 81.522 | 644 | 55 | 5 | 1 | 580 | 1 | 644 | 0.0 | 1037 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0945630 | sp|C0LGH8|Y1634_ARATH | 54.559 | 658 | 206 | 14 | 3 | 580 | 6 | 650 | 0.0 | 661 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000050 | Msa0945630 | 0.820204 | 7.760101e-53 | -8.615850e-47 |
| Msa0006110 | Msa0945630 | 0.805828 | 1.105427e-49 | -8.615850e-47 |
| Msa0010110 | Msa0945630 | 0.809641 | 1.711419e-50 | -8.615850e-47 |
| Msa0024400 | Msa0945630 | 0.800673 | 1.290505e-48 | -8.615850e-47 |
| Msa0026910 | Msa0945630 | 0.802127 | 6.502087e-49 | -8.615850e-47 |
| Msa0032470 | Msa0945630 | 0.814677 | 1.363826e-51 | -8.615850e-47 |
| Msa0034810 | Msa0945630 | 0.812589 | 3.928620e-51 | -8.615850e-47 |
| Msa0038290 | Msa0945630 | -0.825753 | 3.947416e-54 | -8.615850e-47 |
| Msa0045790 | Msa0945630 | 0.807137 | 5.854781e-50 | -8.615850e-47 |
| Msa0046160 | Msa0945630 | 0.807081 | 6.015095e-50 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0945630 | MtrunA17_Chr8g0386631 | 81.522 | 644 | 55 | 5 | 1 | 580 | 1 | 644 | 0.0 | 1037 |
| Msa0945630 | MtrunA17_Chr5g0397651 | 62.963 | 648 | 172 | 8 | 1 | 580 | 1 | 648 | 0.0 | 783 |
| Msa0945630 | MtrunA17_Chr2g0283991 | 38.742 | 302 | 159 | 7 | 290 | 568 | 116 | 414 | 7.15e-59 | 206 |
| Msa0945630 | MtrunA17_Chr1g0193121 | 35.277 | 343 | 169 | 11 | 263 | 568 | 273 | 599 | 3.49e-55 | 200 |
| Msa0945630 | MtrunA17_Chr8g0379101 | 32.481 | 391 | 218 | 10 | 216 | 575 | 308 | 683 | 1.22e-54 | 200 |
| Msa0945630 | MtrunA17_Chr3g0113951 | 33.234 | 337 | 202 | 8 | 263 | 580 | 403 | 735 | 1.04e-51 | 192 |
| Msa0945630 | MtrunA17_Chr3g0105841 | 35.556 | 315 | 185 | 7 | 274 | 573 | 196 | 507 | 9.37e-47 | 174 |
| Msa0945630 | MtrunA17_Chr5g0413291 | 27.242 | 591 | 333 | 23 | 28 | 569 | 34 | 576 | 1.95e-39 | 154 |
| Msa0945630 | MtrunA17_Chr6g0456231 | 27.141 | 619 | 356 | 24 | 1 | 569 | 1 | 574 | 1.21e-36 | 146 |
| Msa0945630 | MtrunA17_Chr4g0073471 | 26.701 | 588 | 334 | 24 | 28 | 569 | 29 | 565 | 3.10e-36 | 145 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0945630 | AT1G63430.3 | 54.559 | 658 | 206 | 14 | 3 | 580 | 6 | 650 | 0.0 | 661 |
| Msa0945630 | AT1G63430.1 | 54.559 | 658 | 206 | 14 | 3 | 580 | 6 | 650 | 0.0 | 661 |
| Msa0945630 | AT1G63430.2 | 52.639 | 682 | 206 | 14 | 3 | 580 | 6 | 674 | 0.0 | 652 |
| Msa0945630 | AT5G41180.1 | 47.496 | 659 | 239 | 16 | 1 | 580 | 9 | 639 | 4.68e-180 | 530 |
| Msa0945630 | AT5G41180.2 | 54.134 | 508 | 163 | 11 | 120 | 580 | 45 | 529 | 1.77e-176 | 517 |
| Msa0945630 | AT4G18640.1 | 36.544 | 353 | 193 | 10 | 247 | 575 | 309 | 654 | 8.15e-56 | 203 |
| Msa0945630 | AT5G58540.3 | 35.826 | 321 | 174 | 9 | 265 | 568 | 5 | 310 | 5.60e-54 | 190 |
| Msa0945630 | AT5G58540.1 | 34.954 | 329 | 182 | 9 | 257 | 568 | 140 | 453 | 2.83e-53 | 192 |
| Msa0945630 | AT5G58540.2 | 34.954 | 329 | 182 | 9 | 257 | 568 | 137 | 450 | 2.85e-53 | 192 |
| Msa0945630 | AT2G40270.2 | 30.500 | 400 | 214 | 13 | 219 | 568 | 68 | 453 | 2.53e-50 | 184 |
Find 195 sgRNAs with CRISPR-Local
Find 675 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACCAGCATCCTTGTAAA+TGG | 0.279164 | 6_3:+24420299 | None:intergenic |
| AAAAGCATAAATGTTTCCTT+CGG | 0.492864 | 6_3:+24419752 | None:intergenic |
| AAAATCGGTGTGTTCGATGG+CGG | 0.606392 | 6_3:+24417996 | None:intergenic |
| AAACAGAAAATCGTAGAATT+TGG | 0.291235 | 6_3:+24423018 | None:intergenic |
| AAAGAGAGTTTATCCAAGAT+TGG | 0.355862 | 6_3:+24423294 | None:intergenic |
| AAATTATTATAGGCATTGCG+CGG | 0.628597 | 6_3:-24420105 | Msa0945630:CDS |
| AAATTGTTATGAAGAGATGA+GGG | 0.626191 | 6_3:-24412642 | Msa0945630:CDS |
| AAATTTGAAGTGCTAGCAGC+AGG | 0.541164 | 6_3:+24421693 | None:intergenic |
| AACTCTGATATAGTAAAAGG+AGG | 0.580646 | 6_3:+24420052 | None:intergenic |
| AAGAAGGGTTCCGGCTATCT+TGG | 0.374192 | 6_3:-24420141 | Msa0945630:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGAGAGTTTATCCAAGAT+TGG | + | chr6_3:24412522-24412541 | None:intergenic | 30.0% | |
| TTGAAGTAGCTGAATTAACA+AGG | - | chr6_3:24412854-24412873 | Msa0945630:intron | 30.0% | |
| TTATGTTTCTTGTGTGAACT+TGG | - | chr6_3:24413167-24413186 | Msa0945630:intron | 30.0% | |
| ATAATTCCCAATTTCCATTC+TGG | + | chr6_3:24413251-24413270 | None:intergenic | 30.0% | |
| AGAATGGAAATTGGGAATTA+TGG | - | chr6_3:24413250-24413269 | Msa0945630:intron | 30.0% | |
| TTCATAAACAGCTTCTTTGA+AGG | + | chr6_3:24413369-24413388 | None:intergenic | 30.0% | |
| TGTTTATGAAGATCCACATA+TGG | - | chr6_3:24413379-24413398 | Msa0945630:intron | 30.0% | |
| CAATTTGACAAAACCATATG+TGG | + | chr6_3:24413395-24413414 | None:intergenic | 30.0% | |
| GGATGTAATTAGACTTTCTT+TGG | - | chr6_3:24413527-24413546 | Msa0945630:intron | 30.0% | |
| ACAGTTTATTTAAGCGTTCA+TGG | - | chr6_3:24413554-24413573 | Msa0945630:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_3 | gene | 24412504 | 24423331 | 24412504 | ID=Msa0945630;Name=Msa0945630 |
| chr6_3 | mRNA | 24412504 | 24423331 | 24412504 | ID=Msa0945630-mRNA-1;Parent=Msa0945630;Name=Msa0945630-mRNA-1;_AED=0.22;_eAED=0.22;_QI=404|0.5|0.57|1|0.66|0.68|19|124|745 |
| chr6_3 | exon | 24412504 | 24412669 | 24412504 | ID=Msa0945630-mRNA-1:exon:7038;Parent=Msa0945630-mRNA-1 |
| chr6_3 | exon | 24414237 | 24414303 | 24414237 | ID=Msa0945630-mRNA-1:exon:7037;Parent=Msa0945630-mRNA-1 |
| chr6_3 | exon | 24414489 | 24414550 | 24414489 | ID=Msa0945630-mRNA-1:exon:7036;Parent=Msa0945630-mRNA-1 |
| chr6_3 | exon | 24416961 | 24417020 | 24416961 | ID=Msa0945630-mRNA-1:exon:7035;Parent=Msa0945630-mRNA-1 |
| chr6_3 | exon | 24417134 | 24417196 | 24417134 | ID=Msa0945630-mRNA-1:exon:7034;Parent=Msa0945630-mRNA-1 |
| chr6_3 | exon | 24417988 | 24418182 | 24417988 | ID=Msa0945630-mRNA-1:exon:7033;Parent=Msa0945630-mRNA-1 |
| chr6_3 | exon | 24418646 | 24418652 | 24418646 | ID=Msa0945630-mRNA-1:exon:7032;Parent=Msa0945630-mRNA-1 |
| chr6_3 | exon | 24418964 | 24419166 | 24418964 | ID=Msa0945630-mRNA-1:exon:7031;Parent=Msa0945630-mRNA-1 |
| chr6_3 | exon | 24419749 | 24419886 | 24419749 | ID=Msa0945630-mRNA-1:exon:7030;Parent=Msa0945630-mRNA-1 |
| chr6_3 | exon | 24420009 | 24420166 | 24420009 | ID=Msa0945630-mRNA-1:exon:7029;Parent=Msa0945630-mRNA-1 |
| chr6_3 | exon | 24420255 | 24420390 | 24420255 | ID=Msa0945630-mRNA-1:exon:7028;Parent=Msa0945630-mRNA-1 |
| chr6_3 | exon | 24420486 | 24420691 | 24420486 | ID=Msa0945630-mRNA-1:exon:7027;Parent=Msa0945630-mRNA-1 |
| chr6_3 | exon | 24420818 | 24421042 | 24420818 | ID=Msa0945630-mRNA-1:exon:7026;Parent=Msa0945630-mRNA-1 |
| chr6_3 | exon | 24421221 | 24421285 | 24421221 | ID=Msa0945630-mRNA-1:exon:7025;Parent=Msa0945630-mRNA-1 |
| chr6_3 | exon | 24421366 | 24421479 | 24421366 | ID=Msa0945630-mRNA-1:exon:7024;Parent=Msa0945630-mRNA-1 |
| chr6_3 | exon | 24421672 | 24421811 | 24421672 | ID=Msa0945630-mRNA-1:exon:7023;Parent=Msa0945630-mRNA-1 |
| chr6_3 | exon | 24421904 | 24422047 | 24421904 | ID=Msa0945630-mRNA-1:exon:7022;Parent=Msa0945630-mRNA-1 |
| chr6_3 | exon | 24422357 | 24422484 | 24422357 | ID=Msa0945630-mRNA-1:exon:7021;Parent=Msa0945630-mRNA-1 |
| chr6_3 | exon | 24422843 | 24423331 | 24422843 | ID=Msa0945630-mRNA-1:exon:7020;Parent=Msa0945630-mRNA-1 |
| chr6_3 | five_prime_UTR | 24422928 | 24423331 | 24422928 | ID=Msa0945630-mRNA-1:five_prime_utr;Parent=Msa0945630-mRNA-1 |
| chr6_3 | CDS | 24422843 | 24422927 | 24422843 | ID=Msa0945630-mRNA-1:cds;Parent=Msa0945630-mRNA-1 |
| chr6_3 | CDS | 24422357 | 24422484 | 24422357 | ID=Msa0945630-mRNA-1:cds;Parent=Msa0945630-mRNA-1 |
| chr6_3 | CDS | 24421904 | 24422047 | 24421904 | ID=Msa0945630-mRNA-1:cds;Parent=Msa0945630-mRNA-1 |
| chr6_3 | CDS | 24421672 | 24421811 | 24421672 | ID=Msa0945630-mRNA-1:cds;Parent=Msa0945630-mRNA-1 |
| chr6_3 | CDS | 24421366 | 24421479 | 24421366 | ID=Msa0945630-mRNA-1:cds;Parent=Msa0945630-mRNA-1 |
| chr6_3 | CDS | 24421221 | 24421285 | 24421221 | ID=Msa0945630-mRNA-1:cds;Parent=Msa0945630-mRNA-1 |
| chr6_3 | CDS | 24420818 | 24421042 | 24420818 | ID=Msa0945630-mRNA-1:cds;Parent=Msa0945630-mRNA-1 |
| chr6_3 | CDS | 24420486 | 24420691 | 24420486 | ID=Msa0945630-mRNA-1:cds;Parent=Msa0945630-mRNA-1 |
| chr6_3 | CDS | 24420255 | 24420390 | 24420255 | ID=Msa0945630-mRNA-1:cds;Parent=Msa0945630-mRNA-1 |
| chr6_3 | CDS | 24420009 | 24420166 | 24420009 | ID=Msa0945630-mRNA-1:cds;Parent=Msa0945630-mRNA-1 |
| chr6_3 | CDS | 24419749 | 24419886 | 24419749 | ID=Msa0945630-mRNA-1:cds;Parent=Msa0945630-mRNA-1 |
| chr6_3 | CDS | 24418964 | 24419166 | 24418964 | ID=Msa0945630-mRNA-1:cds;Parent=Msa0945630-mRNA-1 |
| chr6_3 | CDS | 24418646 | 24418652 | 24418646 | ID=Msa0945630-mRNA-1:cds;Parent=Msa0945630-mRNA-1 |
| chr6_3 | CDS | 24417988 | 24418182 | 24417988 | ID=Msa0945630-mRNA-1:cds;Parent=Msa0945630-mRNA-1 |
| chr6_3 | CDS | 24417134 | 24417196 | 24417134 | ID=Msa0945630-mRNA-1:cds;Parent=Msa0945630-mRNA-1 |
| chr6_3 | CDS | 24416961 | 24417020 | 24416961 | ID=Msa0945630-mRNA-1:cds;Parent=Msa0945630-mRNA-1 |
| chr6_3 | CDS | 24414489 | 24414550 | 24414489 | ID=Msa0945630-mRNA-1:cds;Parent=Msa0945630-mRNA-1 |
| chr6_3 | CDS | 24414237 | 24414303 | 24414237 | ID=Msa0945630-mRNA-1:cds;Parent=Msa0945630-mRNA-1 |
| chr6_3 | CDS | 24412628 | 24412669 | 24412628 | ID=Msa0945630-mRNA-1:cds;Parent=Msa0945630-mRNA-1 |
| chr6_3 | three_prime_UTR | 24412504 | 24412627 | 24412504 | ID=Msa0945630-mRNA-1:three_prime_utr;Parent=Msa0945630-mRNA-1 |
| Gene Sequence |
>Msa0945630 ATGAAGTTGTGTACTTCATTGTTGTTTCTGGGTTTTGTTTCTATGCTTTCTTTTGTGGCTTCTGCTATGGTGGCATCAAATGAAGTCGGGGCGCTTACAACCTTCAAAGAAGCTGTTTATGAAGATCCACATATGGTTTTGTCAAATTGGAATACTTTGGATTCAGATCCTTGTGACTGGAATGGAGTTTCATGCACCGGCACTCGAGATCATAATATTGCATGGCAACAATCTCATTGGAACAATACCGAAAGAATTGAGCGCGTTAAAATCTCTGAAGGTGTTAGATTTGGGAAAGAATCAGTTATCTGGACCAATTCCTCGGGAGATTGGAAATTTGACACAAATTGTAAAAATTCCAATGGTTTGACTGGTAGGTTGCCTTCAGAGCTTGCAAATTTGAAATATCTTCAAGAACTTCGGCTTGATAGGAATAAGCTTGAAGGGCCTGTTCCTGCTGCTAGCACTTCAAATTTTCCTTCAAATATGCATGAATTGTATGCCTCAAATGCAAATTCGACTGGTTTATGTCGTTCGTCGCTTAAAGTTGCAGATTTTTCATATAACTTTTTGGTTGGTAGCATACCAAAATGCTTGGAGTATCTTCCAAGAACTAGCTTTCAAGGGAATTGCCTCCGTGTCAAAGTTGTAAAACAGCGGACTAAAGTTCAATGTGCTGGAGCTTCACCTGCTCAAGGTCATCCAGTAGTGAAACCAAAACACCTGTCTAAAGCTGAACATGAATCAAAGCATGAAGGAGGTTCAAAACCTGCTTGGCTTTTGGCTCTAGAAATAGCAACCGGAACCATGGTTGGTTCTCTCTTTCTGATTGGTATTTTCACTGCTATCCAAAGGTTTAACAACAAATCATCCATCATTATTCCTTGGAAGAAATCCTCAAATTCTGAGATGTTGAAAGATGTGACAAGGTATAGCAGACAAGAGCTTGAGGTGGCTTGTGAAGATTTCAGTAACATAATCGGATCTTCACCGGATAGTGTGGTCTACAAAGGTACAATGAAAGGTGGACCTGAGATTGCTGCAATTTCCCTCTGCATCAAGGAAGAGAATTGGACTGGACACCTTGAACTTTATTTTCAGAGAGAGGTGGCAGACTTGGCAAGGATAAATCATGAGAATACAGGAAAGCTACTAGGCTATTGCAGAGAGAGCAGTCCATTTACAAGGATGCTGGTTTTTGATTATGCATCAAATGGCACTCTTTATGAGCACCTACATTGTTATGAAGAAGGGTTCCGGCTATCTTGGACGCGGCGCATGAAAATTATTATAGGCATTGCGCGGGGACTTAAGTATTTGCACAATGAAATTGACCCTCCTTTTACTATATCAGAGTTGAATTCAAATGCTATATACCTTACAGAAGATTTTTCCCCCAAGTTGGTTGATTTTGAAAGTTGGAAGACGATTCTTGAAAGATCAGAAAAAAATTCTGGTTCTGTTAGCAGCCAAGGTGCTGTTCCAAACTCCCTTCAAGCCCGCCATCTTGATACCGAAGGAAACATTTATGCTTTTGCAGCCAAGGATTATCTTGAATTGCCAGAAGTAATGTCATATTTGGTGGATCCTGAGTTGAAACATTTCGGATATGATGAGCTCAAAGTAATATGTGAGGTGATAACACTTTGTATTAGTCCTGACTCCAATGGATGTCCATCAATGCAAGAGTTGTGCAGCATGCTGGAAAGCAGAATCGACACATCGATAAGTGTGGAGCTCAATTTACTTGGAGTCGAGGGGGAGAGAGAGAGGGAGGGAGTACCGGAGAAGAAGTTGCCGGCGGTGAGAGGGAGTAGCGGAGAAGAAGTCGTCGGCGGTGGGAGGGAGTACCGGAGAAGAATTCGCCGGCGGTGGGATTGGATTGAAGATGATCGGAAAAGTCCTCGCCGTCTCCGCCATCGAACACACCGATTTTCATGGGAGAGTAATGAACTCTTTGCACTTTTCTTGGATGAAACTATGACATACTCATGTGCAGTGTTCAAGGATGAAGATGAAGGCTTGAAAGATACACAAATGAGAAAAATATCTCTTCTGATTGAAAGAATGAGGGAAGAAGACGAGGAAATTGAAGCTGATGGTGCCAATTACCAGCTTGAAATGATGAGGTACCAGTCTACATTGCTTGGCTCCTATCAAACTGTGGAGCTGATTGATACCTTTATGAATTACCAGAGTGGAAATTGTTATGAAGAGATGAGGGAAATATGGCGTTGA |
| Protein sequence |
>Msa0945630 MKLCTSLLFLGFVSMLSFVASAMVASNEVGALTTFKEAVYEDPHMVLSNWNTLDSDPCDWNGVSCTGTRDHNIAWQQSHWNNTERIERVKISEGVRFGKESVIWTNSSGDWKFDTNCKNSNGLTGRLPSELANLKYLQELRLDRNKLEGPVPAASTSNFPSNMHELYASNANSTGLCRSSLKVADFSYNFLVGSIPKCLEYLPRTSFQGNCLRVKVVKQRTKVQCAGASPAQGHPVVKPKHLSKAEHESKHEGGSKPAWLLALEIATGTMVGSLFLIGIFTAIQRFNNKSSIIIPWKKSSNSEMLKDVTRYSRQELEVACEDFSNIIGSSPDSVVYKGTMKGGPEIAAISLCIKEENWTGHLELYFQREVADLARINHENTGKLLGYCRESSPFTRMLVFDYASNGTLYEHLHCYEEGFRLSWTRRMKIIIGIARGLKYLHNEIDPPFTISELNSNAIYLTEDFSPKLVDFESWKTILERSEKNSGSVSSQGAVPNSLQARHLDTEGNIYAFAAKDYLELPEVMSYLVDPELKHFGYDELKVICEVITLCISPDSNGCPSMQELCSMLESRIDTSISVELNLLGVEGEREREGVPEKKLPAVRGSSGEEVVGGGREYRRRIRRRWDWIEDDRKSPRRLRHRTHRFSWESNELFALFLDETMTYSCAVFKDEDEGLKDTQMRKISLLIERMREEDEEIEADGANYQLEMMRYQSTLLGSYQTVELIDTFMNYQSGNCYEEMREIWR* |
