Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0154180 | sp|Q9FMX6|DJC76_ARATH | 33.990 | 203 | 117 | 5 | 1 | 192 | 263 | 459 | 2.36e-23 | 99.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0154180 | G7IBD4 | 92.268 | 194 | 14 | 1 | 1 | 194 | 267 | 459 | 1.46e-125 | 371 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0014410 | Msa0154180 | 0.859453 | 4.093757e-63 | -8.615850e-47 |
| Msa0020630 | Msa0154180 | 0.802009 | 6.873136e-49 | -8.615850e-47 |
| Msa0063760 | Msa0154180 | 0.934585 | 3.216326e-96 | -8.615850e-47 |
| Msa0064550 | Msa0154180 | 0.835240 | 1.882897e-56 | -8.615850e-47 |
| Msa0109490 | Msa0154180 | 0.839864 | 1.227707e-57 | -8.615850e-47 |
| Msa0154180 | Msa0154200 | 0.943018 | 2.558421e-102 | -8.615850e-47 |
| Msa0154180 | Msa0154220 | 0.870346 | 1.564298e-66 | -8.615850e-47 |
| Msa0154180 | Msa0154240 | 0.845994 | 2.868819e-59 | -8.615850e-47 |
| Msa0154180 | Msa0154270 | 0.868338 | 7.034847e-66 | -8.615850e-47 |
| Msa0154180 | Msa0155610 | 0.833803 | 4.323198e-56 | -8.615850e-47 |
| Msa0154180 | Msa0224370 | 0.806323 | 8.696628e-50 | -8.615850e-47 |
| Msa0154180 | Msa0260190 | 0.825582 | 4.333388e-54 | -8.615850e-47 |
| Msa0154180 | Msa0265580 | 0.810334 | 1.214081e-50 | -8.615850e-47 |
| Msa0154180 | Msa0303440 | 0.811481 | 6.853371e-51 | -8.615850e-47 |
| Msa0154180 | Msa0303500 | 0.804333 | 2.272319e-49 | -8.615850e-47 |
| Msa0154180 | Msa0344680 | 0.804147 | 2.484023e-49 | -8.615850e-47 |
| Msa0154180 | Msa0354390 | 0.835361 | 1.754417e-56 | -8.615850e-47 |
| Msa0154180 | Msa0377280 | 0.801441 | 8.991781e-49 | -8.615850e-47 |
| Msa0154180 | Msa0392050 | 0.808264 | 3.373999e-50 | -8.615850e-47 |
| Msa0154180 | Msa0401340 | 0.840762 | 7.150440e-58 | -8.615850e-47 |
| Msa0154180 | Msa0418430 | 0.814328 | 1.629236e-51 | -8.615850e-47 |
| Msa0154180 | Msa0448120 | 0.833278 | 5.846289e-56 | -8.615850e-47 |
| Msa0154180 | Msa0462590 | 0.808282 | 3.344021e-50 | -8.615850e-47 |
| Msa0154180 | Msa0469950 | 0.809377 | 1.950516e-50 | -8.615850e-47 |
| Msa0154180 | Msa0525270 | 0.801608 | 8.309980e-49 | -8.615850e-47 |
| Msa0154180 | Msa0530950 | 0.817259 | 3.618643e-52 | -8.615850e-47 |
| Msa0154180 | Msa0584280 | 0.830564 | 2.736245e-55 | -8.615850e-47 |
| Msa0154180 | Msa0600820 | 0.815631 | 8.375195e-52 | -8.615850e-47 |
| Msa0154180 | Msa0627050 | 0.825276 | 5.119140e-54 | -8.615850e-47 |
| Msa0154180 | Msa0628100 | 0.810442 | 1.150431e-50 | -8.615850e-47 |
| Msa0154180 | Msa0646190 | 0.812184 | 4.817517e-51 | -8.615850e-47 |
| Msa0154180 | Msa0676100 | 0.810122 | 1.348420e-50 | -8.615850e-47 |
| Msa0154180 | Msa0775580 | 0.800995 | 1.109575e-48 | -8.615850e-47 |
| Msa0154180 | Msa0806720 | 0.803567 | 3.277244e-49 | -8.615850e-47 |
| Msa0154180 | Msa0806760 | 0.810060 | 1.390865e-50 | -8.615850e-47 |
| Msa0154180 | Msa0828570 | 0.805697 | 1.177702e-49 | -8.615850e-47 |
| Msa0154180 | Msa0899700 | 0.819190 | 1.322714e-52 | -8.615850e-47 |
| Msa0154180 | Msa0945960 | 0.802997 | 4.301236e-49 | -8.615850e-47 |
| Msa0154180 | Msa0964680 | 0.804876 | 1.749996e-49 | -8.615850e-47 |
| Msa0154180 | Msa0968780 | 0.808962 | 2.392913e-50 | -8.615850e-47 |
| Msa0154180 | Msa0984000 | 0.847380 | 1.199580e-59 | -8.615850e-47 |
| Msa0154180 | Msa1004210 | 0.810533 | 1.099271e-50 | -8.615850e-47 |
| Msa0154180 | Msa1046610 | 0.816886 | 4.389437e-52 | -8.615850e-47 |
| Msa0154180 | Msa1144730 | 0.806617 | 7.541775e-50 | -8.615850e-47 |
| Msa0154180 | Msa1182830 | 0.828556 | 8.418891e-55 | -8.615850e-47 |
| Msa0154180 | Msa1241820 | 0.806173 | 9.354506e-50 | -8.615850e-47 |
| Msa0154180 | Msa1272950 | 0.804333 | 2.271717e-49 | -8.615850e-47 |
| Msa0154180 | Msa1306190 | 0.820943 | 5.250563e-53 | -8.615850e-47 |
| Msa0154180 | Msa1306200 | 0.833676 | 4.652102e-56 | -8.615850e-47 |
| Msa0154180 | Msa1317030 | 0.805017 | 1.635416e-49 | -8.615850e-47 |
| Msa0154180 | Msa1349810 | 0.801307 | 9.578865e-49 | -8.615850e-47 |
| Msa0154180 | Msa1378470 | 0.806772 | 6.994241e-50 | -8.615850e-47 |
| Msa0154180 | Msa1394900 | 0.815442 | 9.227890e-52 | -8.615850e-47 |
| Msa0154180 | Msa1421870 | 0.810725 | 9.993121e-51 | -8.615850e-47 |
| Msa0154180 | Msa1459540 | 0.812755 | 3.614849e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0154180 | MtrunA17_Chr1g0164721 | 92.268 | 194 | 14 | 1 | 1 | 194 | 285 | 477 | 4.18e-129 | 371 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0154180 | AT5G23240.1 | 33.990 | 203 | 117 | 5 | 1 | 192 | 263 | 459 | 2.40e-24 | 99.4 |
Find 43 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCAGGATGAATGGAATTTA+TGG | 0.158588 | 1_4:+18523011 | None:intergenic |
| CAATTTCTAATTGGTTATAT+TGG | 0.226095 | 1_4:-18523173 | Msa0154180:CDS |
| CTCTTTGCAGCTGCATCTCT+TGG | 0.264299 | 1_4:+18523072 | None:intergenic |
| TCTCTTGGCTTACTAAGATT+TGG | 0.268758 | 1_4:+18523087 | None:intergenic |
| CCATTCATCCTGAGGAATAT+TGG | 0.276497 | 1_4:-18523002 | Msa0154180:CDS |
| ACTGGCAATCCCCATCTAAT+TGG | 0.277395 | 1_4:+18522844 | None:intergenic |
| CAATTAGATCAATTTCTAAT+TGG | 0.293169 | 1_4:-18523182 | Msa0154180:CDS |
| CCAGTGGTTACTTCATTGTT+TGG | 0.318448 | 1_4:-18522826 | Msa0154180:CDS |
| TTGGTGCGAATTGCACTATT+TGG | 0.322832 | 1_4:+18523147 | None:intergenic |
| ATGAAAATAGTCCAATTAGA+TGG | 0.341134 | 1_4:-18522855 | Msa0154180:CDS |
| TGGAAATTGTTAACAGCTCT+TGG | 0.381002 | 1_4:-18522726 | Msa0154180:CDS |
| TCAGGATGAATGGAATTTAT+GGG | 0.397759 | 1_4:+18523012 | None:intergenic |
| GAAGGAAGAACAGTAGTTGA+TGG | 0.402925 | 1_4:+18522976 | None:intergenic |
| AGTTGAGTTGAAAGAGCATT+GGG | 0.412413 | 1_4:-18522764 | Msa0154180:CDS |
| TCGATTTCTATTTCCAATGT+TGG | 0.441877 | 1_4:+18522639 | None:intergenic |
| GGTTACTTCATTGTTTGGTA+TGG | 0.442830 | 1_4:-18522821 | Msa0154180:CDS |
| CCAATATTCCTCAGGATGAA+TGG | 0.445444 | 1_4:+18523002 | None:intergenic |
| TTGGGGAGGTTCTCTAGCCT+TGG | 0.460364 | 1_4:-18522746 | Msa0154180:CDS |
| TGAAAATAGTCCAATTAGAT+GGG | 0.463058 | 1_4:-18522854 | Msa0154180:CDS |
| GAAAATGTAGGCTTCTCAAT+TGG | 0.464770 | 1_4:+18522925 | None:intergenic |
| GCAGCTACATGGTATGTGAT+TGG | 0.477504 | 1_4:-18522685 | Msa0154180:CDS |
| AAGGGTTGCTGTAGAATTTG+TGG | 0.483426 | 1_4:-18522662 | Msa0154180:CDS |
| GGCTTACTAAGATTTGGATC+AGG | 0.486859 | 1_4:+18523093 | None:intergenic |
| TGTTTGGTATGGCTGCAGTT+AGG | 0.496837 | 1_4:-18522810 | Msa0154180:CDS |
| CAGTTGAGTTGAAAGAGCAT+TGG | 0.510120 | 1_4:-18522765 | Msa0154180:CDS |
| GTAGTAGTACTAGTTGAAGA+AGG | 0.518499 | 1_4:+18522958 | None:intergenic |
| CTCTTGGTTGCAATATATGT+TGG | 0.521916 | 1_4:-18522710 | Msa0154180:CDS |
| TTCTTCCCTTGAGAAAATGT+AGG | 0.528643 | 1_4:+18522913 | None:intergenic |
| GATGGAGTCCAATATTCCTC+AGG | 0.534720 | 1_4:+18522994 | None:intergenic |
| CTACATGGTATGTGATTGGA+AGG | 0.538431 | 1_4:-18522681 | Msa0154180:CDS |
| GTAGAATTTGTGGCCAACAT+TGG | 0.550242 | 1_4:-18522652 | Msa0154180:CDS |
| CAGGATGAATGGAATTTATG+GGG | 0.557366 | 1_4:+18523013 | None:intergenic |
| ATATGTTGGTAGCAGCTACA+TGG | 0.578806 | 1_4:-18522696 | Msa0154180:CDS |
| AGAGCTGTTAACAATTTCCA+AGG | 0.595776 | 1_4:+18522729 | None:intergenic |
| GCAGTTAGGTTACATGAAGT+AGG | 0.596129 | 1_4:-18522796 | Msa0154180:CDS |
| AATTAGATGGGGATTGCCAG+TGG | 0.600248 | 1_4:-18522842 | Msa0154180:CDS |
| CATAAATTCCATTCATCCTG+AGG | 0.607786 | 1_4:-18523010 | Msa0154180:CDS |
| CCAAACAATGAAGTAACCAC+TGG | 0.612811 | 1_4:+18522826 | None:intergenic |
| AGGATGAATGGAATTTATGG+GGG | 0.638502 | 1_4:+18523014 | None:intergenic |
| GAGTTGAAAGAGCATTGGGG+AGG | 0.639356 | 1_4:-18522760 | Msa0154180:CDS |
| GAAAATAGTCCAATTAGATG+GGG | 0.640775 | 1_4:-18522853 | Msa0154180:CDS |
| GTTGAGTTGAAAGAGCATTG+GGG | 0.697316 | 1_4:-18522763 | Msa0154180:CDS |
| TACATGGTATGTGATTGGAA+GGG | 0.767981 | 1_4:-18522680 | Msa0154180:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAATTAGATCAATTTCTAAT+TGG | - | chr1_4:18522652-18522671 | Msa0154180:CDS | 20.0% |
| !! | CAATTTCTAATTGGTTATAT+TGG | - | chr1_4:18522661-18522680 | Msa0154180:CDS | 20.0% |
| ! | ATGAAAATAGTCCAATTAGA+TGG | - | chr1_4:18522979-18522998 | Msa0154180:CDS | 25.0% |
| ! | TGAAAATAGTCCAATTAGAT+GGG | - | chr1_4:18522980-18522999 | Msa0154180:CDS | 25.0% |
| !! | ATTTATGGGGGTTTTATGTT+TGG | + | chr1_4:18522811-18522830 | None:intergenic | 30.0% |
| TCAGGATGAATGGAATTTAT+GGG | + | chr1_4:18522825-18522844 | None:intergenic | 30.0% | |
| GAAAATAGTCCAATTAGATG+GGG | - | chr1_4:18522981-18523000 | Msa0154180:CDS | 30.0% | |
| TCGATTTCTATTTCCAATGT+TGG | + | chr1_4:18523198-18523217 | None:intergenic | 30.0% | |
| ! | CACAACTTTTGTCATGCTTT+TGG | + | chr1_4:18522709-18522728 | None:intergenic | 35.0% |
| TCTCTTGGCTTACTAAGATT+TGG | + | chr1_4:18522750-18522769 | None:intergenic | 35.0% | |
| AGGATGAATGGAATTTATGG+GGG | + | chr1_4:18522823-18522842 | None:intergenic | 35.0% | |
| CAGGATGAATGGAATTTATG+GGG | + | chr1_4:18522824-18522843 | None:intergenic | 35.0% | |
| CTCAGGATGAATGGAATTTA+TGG | + | chr1_4:18522826-18522845 | None:intergenic | 35.0% | |
| CATAAATTCCATTCATCCTG+AGG | - | chr1_4:18522824-18522843 | Msa0154180:CDS | 35.0% | |
| GTAGTAGTACTAGTTGAAGA+AGG | + | chr1_4:18522879-18522898 | None:intergenic | 35.0% | |
| GAAAATGTAGGCTTCTCAAT+TGG | + | chr1_4:18522912-18522931 | None:intergenic | 35.0% | |
| ! | AGAAGCCTACATTTTCTCAA+GGG | - | chr1_4:18522916-18522935 | Msa0154180:CDS | 35.0% |
| TTCTTCCCTTGAGAAAATGT+AGG | + | chr1_4:18522924-18522943 | None:intergenic | 35.0% | |
| GGTTACTTCATTGTTTGGTA+TGG | - | chr1_4:18523013-18523032 | Msa0154180:CDS | 35.0% | |
| !! | AGTTGAGTTGAAAGAGCATT+GGG | - | chr1_4:18523070-18523089 | Msa0154180:CDS | 35.0% |
| AGAGCTGTTAACAATTTCCA+AGG | + | chr1_4:18523108-18523127 | None:intergenic | 35.0% | |
| TGGAAATTGTTAACAGCTCT+TGG | - | chr1_4:18523108-18523127 | Msa0154180:CDS | 35.0% | |
| CTCTTGGTTGCAATATATGT+TGG | - | chr1_4:18523124-18523143 | Msa0154180:CDS | 35.0% | |
| TACATGGTATGTGATTGGAA+GGG | - | chr1_4:18523154-18523173 | Msa0154180:CDS | 35.0% | |
| TTGGTGCGAATTGCACTATT+TGG | + | chr1_4:18522690-18522709 | None:intergenic | 40.0% | |
| GGCTTACTAAGATTTGGATC+AGG | + | chr1_4:18522744-18522763 | None:intergenic | 40.0% | |
| CCAATATTCCTCAGGATGAA+TGG | + | chr1_4:18522835-18522854 | None:intergenic | 40.0% | |
| CCATTCATCCTGAGGAATAT+TGG | - | chr1_4:18522832-18522851 | Msa0154180:CDS | 40.0% | |
| GAAGGAAGAACAGTAGTTGA+TGG | + | chr1_4:18522861-18522880 | None:intergenic | 40.0% | |
| ! | GAGAAGCCTACATTTTCTCA+AGG | - | chr1_4:18522915-18522934 | Msa0154180:CDS | 40.0% |
| CCAAACAATGAAGTAACCAC+TGG | + | chr1_4:18523011-18523030 | None:intergenic | 40.0% | |
| CCAGTGGTTACTTCATTGTT+TGG | - | chr1_4:18523008-18523027 | Msa0154180:CDS | 40.0% | |
| GCAGTTAGGTTACATGAAGT+AGG | - | chr1_4:18523038-18523057 | Msa0154180:CDS | 40.0% | |
| !! | CAGTTGAGTTGAAAGAGCAT+TGG | - | chr1_4:18523069-18523088 | Msa0154180:CDS | 40.0% |
| !! | GTTGAGTTGAAAGAGCATTG+GGG | - | chr1_4:18523071-18523090 | Msa0154180:CDS | 40.0% |
| !! | ATATGTTGGTAGCAGCTACA+TGG | - | chr1_4:18523138-18523157 | Msa0154180:CDS | 40.0% |
| CTACATGGTATGTGATTGGA+AGG | - | chr1_4:18523153-18523172 | Msa0154180:CDS | 40.0% | |
| AAGGGTTGCTGTAGAATTTG+TGG | - | chr1_4:18523172-18523191 | Msa0154180:CDS | 40.0% | |
| GTAGAATTTGTGGCCAACAT+TGG | - | chr1_4:18523182-18523201 | Msa0154180:CDS | 40.0% | |
| GATGGAGTCCAATATTCCTC+AGG | + | chr1_4:18522843-18522862 | None:intergenic | 45.0% | |
| ACTGGCAATCCCCATCTAAT+TGG | + | chr1_4:18522993-18523012 | None:intergenic | 45.0% | |
| AATTAGATGGGGATTGCCAG+TGG | - | chr1_4:18522992-18523011 | Msa0154180:CDS | 45.0% | |
| TGTTTGGTATGGCTGCAGTT+AGG | - | chr1_4:18523024-18523043 | Msa0154180:CDS | 45.0% | |
| GCAGCTACATGGTATGTGAT+TGG | - | chr1_4:18523149-18523168 | Msa0154180:CDS | 45.0% | |
| CTCTTTGCAGCTGCATCTCT+TGG | + | chr1_4:18522765-18522784 | None:intergenic | 50.0% | |
| !! | GAGTTGAAAGAGCATTGGGG+AGG | - | chr1_4:18523074-18523093 | Msa0154180:CDS | 50.0% |
| TTGGGGAGGTTCTCTAGCCT+TGG | - | chr1_4:18523088-18523107 | Msa0154180:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_4 | gene | 18522636 | 18523220 | 18522636 | ID=Msa0154180;Name=Msa0154180 |
| chr1_4 | mRNA | 18522636 | 18523220 | 18522636 | ID=Msa0154180-mRNA-1;Parent=Msa0154180;Name=Msa0154180-mRNA-1;_AED=0.23;_eAED=0.23;_QI=0|-1|0|1|-1|1|1|0|194 |
| chr1_4 | exon | 18522636 | 18523220 | 18522636 | ID=Msa0154180-mRNA-1:exon:6846;Parent=Msa0154180-mRNA-1 |
| chr1_4 | CDS | 18522636 | 18523220 | 18522636 | ID=Msa0154180-mRNA-1:cds;Parent=Msa0154180-mRNA-1 |
| Gene Sequence |
| Protein sequence |
